Enhancing visible light-induced 3D bioprinting: alternating extruded support materials for bioink gelation

Takashi Kotani, Takehito Hananouchi, Shinji Sakai

Biomedical Materials　Vol. 20　2025/05/01　Research paper (scientific journal)

Synthesis and application of phenol-grafted rhamnan sulfate for 3D bioprinting

Ryota Goto, Masahiro Terasawa, Masaru Kojima, Koichi Matsuda, Kaoru Nishiura, Shinji Sakai

Journal of Biomaterials Science, Polymer Edition　Vol. 36　No. 6　p. 779-795　2025/04/13　Research paper (scientific journal)

Photo- and Schiff Base-Crosslinkable Chitosan/Oxidized Glucomannan Composite Hydrogel for 3D Bioprinting

Mitsuyuki Hidaka, Shinji Sakai

Polysaccharides　Vol. 6　No. 1　2025/03/04　Research paper (scientific journal)

Development of on-chip cell domes using Ca-alginate hydrogel shells for non-adherent cell studies

Shinji Sakai, Hiroyuki Fujiwara, Ryotaro Kazama, Riki Toita, Satoshi Fujita

Lab on a Chip　Vol. 25　p. 3971-3978　2025　Research paper (scientific journal)

Cell Dome-based transfection array for non-adherent suspension cells

Ryotaro Kazama, Satoshi Fujita, Shinji Sakai

Biochemical Engineering Journal　Vol. 213　2025/01　Research paper (scientific journal)

Development of Hemispherical 3D Models of Human Brain and B Cell Lymphomas Using On-Chip Cell Dome System

Ryotaro Kazama, Rina Ishikawa, Shinji Sakai

Bioengineering　Vol. 11　No. 12　2024/12/23　Research paper (scientific journal)

Enzymatically cross-linkable sulfated bacterial polyglucuronic acid as an affinity-based carrier of FGF-2 for therapeutic angiogenesis

Ryota Goto, Shinji Sakai, Cédric Delattre, Emmanuel Petit, Redouan El Boutachfaiti, Masaki Nakahata

Journal of Bioscience and Bioengineering　Vol. 138　No. 6　p. 541-547　2024/12　Research paper (scientific journal)

Machine learning-based prediction and optimisation framework for as-extruded cell viability in extrusion-based 3D bioprinting

Colin Zhang, Kelum Chamara Manoj Lakmal Elvitigala, Wildan Mubarok, Yasunori Okano, Shinji Sakai

Virtual and Physical Prototyping　Vol. 19　No. 1　2024/09/11　Research paper (scientific journal)

Cell Cycle Modulation through Physical Confinement in Micrometer-Thick Hydrogel Sheaths

Wildan Mubarok, Kelum Chamara Manoj Lakmal Elvitigala, Hiroto Nakaya, Tomoki Hotta, Shinji Sakai

Langmuir　Vol. 40　No. 35　p. 18717-18726　2024/08/21　Research paper (scientific journal)

Hyperconfined bio-inspired Polymers in Integrative Flow-Through Systems for Highly Selective Removal of Heavy Metal Ions

Masaki Nakahata, Ai Sumiya, Yuka Ikemoto, Takashi Nakamura, Anastasia Dudin, Julius Schwieger, Akihisa Yamamoto, Shinji Sakai, Stefan Kaufmann, Motomu Tanaka

Nature Communications　Vol. 15　No. 1　2024/07/11　Research paper (scientific journal)

Phototuning of Hyaluronic‐Acid‐Based Hydrogel Properties to Control Network Formation in Human Vascular Endothelial Cells

Kelum Chamara Manoj Lakmal Elvitigala, Lakshmi Mohan, Wildan Mubarok, Shinji Sakai

Advanced Healthcare Materials　2024/07　Research paper (scientific journal)

Hydrogels with Ultrasound-Treated Hyaluronic Acid Regulate CD44-Mediated Angiogenic Potential of Human Vascular Endothelial Cells In Vitro

Kelum Chamara Manoj Lakmal Elvitigala, Wildan Mubarok, Shinji Sakai

Biomolecules　Vol. 14　No. 5　p. 604-604　2024/05/20　Research paper (scientific journal)

3D Bioprinting of Sugar Beet Pectin through Horseradish Peroxidase-Catalyzed Cross-Linking

Wildan Mubarok, Colin Zhang, Shinji Sakai

ACS Applied Bio Materials　Vol. 7　No. 5　p. 3506-3514　2024/05/02　Research paper (scientific journal)

Characterization of Chitosan Hydrogels Obtained through Phenol and Tripolyphosphate Anionic Crosslinking

Mitsuyuki Hidaka, Masaru Kojima, Shinji Sakai, Cédric Delattre

Polymers　Vol. 16　No. 9　2024/05　Research paper (scientific journal)

Transduction and Genome Editing of the Heart with Adeno-Associated Viral Vectors Loaded onto Electrospun Polydioxanone Nonwoven Fabrics

Kotoko Furuno, Keiichiro Suzuki, Shinji Sakai

Biomolecules　2024/04/22　Research paper (scientific journal)

Enzymatically gellable chitosan inks with enhanced printability by chitosan nanofibers for 3D printing of wound dressings

Shinji Sakai, Shota Yamamoto, Ryo Hirami, Mitsuyuki Hidaka, Kelum Chamara Manoj Lakmal Elvitigala

European Polymer Journal　Vol. 210　p. 112960-112960　2024/04　Research paper (scientific journal)

Effect of cell adhesiveness of Cell Dome shell on enclosed HeLa cells

Ryotaro Kazama, Shinji Sakai

Journal of Bioscience and Bioengineering　2024/04　Research paper (scientific journal)

Micromixer driven by bubble-induced acoustic microstreaming for multi-ink 3D bioprinting

Mitsuyuki Hidaka, Masaru Kojima, Shinji Sakai

Lab on a Chip　2024　Research paper (scientific journal)

Local delivery of adeno-associated viral vectors with electrospun gelatin nanofiber mats.

Kotoko Furuno, Kelum Chamara Manoj Lakmal Elvitigala, Keiichiro Suzuki, Shinji Sakai

Journal of biomedical materials research. Part B, Applied biomaterials　2023/10/30　Research paper (scientific journal)

Horseradish Peroxidase-Mediated Bioprinting via Bioink Gelation by Alternately Extruded Support Material

Takashi Kotani, Wildan Mubarok, Takehito Hananouchi, Shinji Sakai

ACS Biomaterials Science & Engineering　Vol. 9　No. 10　p. 5804-5812　2023/09/22　Research paper (scientific journal)

Visible light photocrosslinking of sugar beet pectin for 3D bioprinting applications.

Wildan Mubarok, Kelum Chamara Manoj Lakmal Elvitigala, Takashi Kotani, Shinji Sakai

Carbohydrate polymers　Vol. 316　p. 121026-121026　2023/09/15　Research paper (scientific journal)

Tuning the crosslinking and degradation of hyaluronic acid/gelatin hydrogels using hydrogen peroxide for muscle cell sheet fabrication.

Kelum Chamara Manoj Lakmal Elvitigala, Wildan Mubarok, Shinji Sakai

Soft Matter　Vol. 19　No. 31　p. 5880-5887　2023/08/09　Research paper (scientific journal)

Fabrication of cell-laden microbeads and microcapsules composed of bacterial polyglucuronic acid.

Ryota Goto, Masaki Nakahata, Cédric Delattre, Emmanuel Petit, Redouan El Boutachfaiti, Shinji Sakai

International journal of biological macromolecules　Vol. 244　p. 125481-125481　2023/07/31　Research paper (scientific journal)

Mammalian-specific decellularized matrices derived bioink for bioengineering of liver tissue analogues: A review

Mir TA, Nakamura M, Sakai S, Iwanaga S, Wani SI, Alzhrani A, Arai K, Mir BA, Kazmi S, Assiri AM, Broering DC

International Journal of Bioprinting　Vol. 9　No. 3　p. 714-714　2023/03　Research paper (scientific journal)

Cell Dome as an Evaluation Platform for Organized HepG2 Cells.

Ryotaro Kazama, Satoshi Fujita, Shinji Sakai

Cells　Vol. 12　No. 1　2022/12/23　Research paper (scientific journal)

Phenol-Grafted Alginate Sulfate Hydrogel as an Injectable FGF-2 Carrier

Ryota Goto, Masaki Nakahata, Shinji Sakai

Gels　Vol. 8　No. 12　2022/12/12　Research paper (scientific journal)

Human Umbilical Vein Endothelial Cells Form a Network on a Hyaluronic Acid/Gelatin Composite Hydrogel Moderately Crosslinked and Degraded by Hydrogen Peroxide.

Kelum Chamara Manoj Lakmal Elvitigala, Wildan Mubarok, Shinji Sakai

Polymers　Vol. 14　No. 22　2022/11/20　Research paper (scientific journal)

Design and Fabrication of Mature Engineered Pre-Cardiac Tissue Utilizing 3D Bioprinting Technology and Enzymatically Crosslinking Hydrogel

Shintaroh Iwanaga, Yuta Hamada, Yoshinari Tsukamoto, Kenichi Arai, Taketoshi Kurooka, Shinji Sakai, Makoto Nakamura

Materials　Vol. 15　No. 22　p. 7928-7928　2022/11/09　Research paper (scientific journal)

Gelatin-Based Electrospun Nanofibers Cross-Linked Using Horseradish Peroxidase for Plasmid DNA Delivery

Kotoko Furuno, Keiichiro Suzuki, Shinji Sakai

Biomolecules　Vol. 12　No. 11　2022/11/04　Research paper (scientific journal)

Interaction of alginate with nano-hydroxyapatite-collagen using strontium provides suitable osteogenic platform.

Ayla Hassani, Çığır Biray Avci, Sajed Nazif Kerdar, Hassan Amini, Meisam Amini, Mahdi Ahmadi, Shinji Sakai, Bakiye Goker Bagca, Neslihan Pınar Ozates, Reza Rahbarghazi, Ali Baradar Khoshfetrat

Journal of nanobiotechnology　Vol. 20　No. 1　p. 310-310　2022/06/28　Research paper (scientific journal)

Tuning Myogenesis by Controlling Gelatin Hydrogel Properties through Hydrogen Peroxide-Mediated Cross-Linking and Degradation.

Wildan Mubarok, Kelum Chamara Manoj Lakmal Elvitigala, Shinji Sakai

Gels (Basel, Switzerland)　Vol. 8　No. 6　2022/06/17　Research paper (scientific journal)

One-Step FRESH Bioprinting of Low-Viscosity Silk Fibroin Inks.

Shinji Sakai, Takahiro Morita

ACS biomaterials science & engineering　Vol. 8　No. 6　p. 2589-2597　2022/06/13　Research paper (scientific journal)

A Bio-synthetic Hybrid Hydrogel Formed under Physiological Conditions Consisting of Mucin and a Synthetic Polymer Carrying Boronic Acid.

Masaki Nakahata, Naoki Tominaga, Keishi Saito, Keita Nishiyama, Yuya Tanino, Kiyoshiro Saiki, Masaru Kojima, Shinji Sakai

Macromolecular bioscience　Vol. 22　No. 6　2022/06　Research paper (scientific journal)

Nematode surface functionalization with hydrogel sheaths tailored in situ.

Wildan Mubarok, Masaki Nakahata, Masaru Kojima, Shinji Sakai

Materials today. Bio　Vol. 15　p. 100328-100328　2022/06　Research paper (scientific journal)

Automated Microhand System for Measuring Cell Stiffness By Using Two Plate End-Effectors

Masahiro Kawakami, Masaru Kojima, Yuma Masuda, Yasushi Mae, Takato Horii, Takayuki Nagai, Masaki Nakahata, Shinji Sakai, Tatsuo Arai

IEEE Robotics and Automation Letters　Vol. 7　No. 2　p. 2385-2390　2022/04　Research paper (scientific journal)

Modulation of Cell-Cycle Progression by Hydrogen Peroxide-Mediated Cross-Linking and Degradation of Cell-Adhesive Hydrogels

Wildan Mubarok, Kelum Chamara Manoj Lakmal Elvitigala, Masaki Nakahata, Masaru Kojima, Shinji Sakai

Cells　Vol. 11　No. 5　2022/03/01　Research paper (scientific journal)

Collagen and nano-hydroxyapatite interactions in alginate-based microcapsule provide an appropriate osteogenic microenvironment for modular bone tissue formation.

Ayla Hassani, Ali Baradar Khoshfetrat, Reza Rahbarghazi, Shinji Sakai

Carbohydrate polymers　Vol. 277　p. 118807-118807　2022/02/01　Research paper (scientific journal)

Development of phenol-grafted polyglucuronic acid and its application to extrusion-based bioprinting inks

Shinji Sakai, Takashi Kotani, Ryohei Harada, Ryota Goto, Takahiro Morita, Soukaina Bouissil, Pascal Dubessay, Guillaume Pierre, Philippe Michaud, Redouan El Boutachfaiti, Masaki Nakahata, Masaru Kojima, Emmanuel Petit, Cédric Delattre

Carbohydrate Polymers　Vol. 277　p. 118820-118820　2022/02/01　Research paper (scientific journal)

Freeform 3D Bioprinting Involving Ink Gelation by Cascade Reaction of Oxidase and Peroxidase: A Feasibility Study Using Hyaluronic Acid-Based Ink.

Shinji Sakai, Ryohei Harada, Takashi Kotani

Biomolecules　Vol. 11　No. 12　2021/12/20　Research paper (scientific journal)

Visible light-curable chitosan ink for extrusion-based and vat polymerization-based 3d bioprintings

Mitsuyuki Hidaka, Masaru Kojima, Masaki Nakahata, Shinji Sakai

Polymers　Vol. 13　No. 9　2021/05/01　Research paper (scientific journal)

Influence of Hydrogen Peroxide-Mediated Cross-Linking and Degradation on Cell-Adhesive Gelatin Hydrogels

Wildan Mubarok, Yanfei Qu, Shinji Sakai

ACS Applied Bio Materials　Vol. 4　No. 5　p. 4184-4190　2021/04/14　Research paper (scientific journal)

Silk fibroin nanofibers: a promising ink additive for extrusion three-dimensional bioprinting

S. Sakai, A. Yoshii, S. Sakurai, K. Horii, O. Nagasuna

Materials Today Bio　Vol. 8　2020/09/01　Research paper (scientific journal)

Gelatin-Based Electrospun Fibers Insolubilized by Horseradish Peroxidase-Catalyzed Cross-Linking for Biomedical Applications.

Kotoko Furuno, Jing Wang, Keiichiro Suzuki, Masaki Nakahata, Shinji Sakai

ACS omega　Vol. 5　No. 33　p. 21254-21259　2020/08/25　Research paper (scientific journal)

Extrusion-Based Bioprinting through Glucose-Mediated Enzymatic Hydrogelation.

Enkhtuul Gantumur, Masaki Nakahata, Masaru Kojima, Shinji Sakai

International journal of bioprinting　Vol. 6　No. 1　p. 250-250　2020　Research paper (scientific journal)

Inkjet micropatterning through horseradish peroxidase-mediated hydrogelation for controlled cell immobilization and microtissue fabrication

Gantumur, Enkhtuul, Kimura, Miyu, Taya, Masahito, Horie, Masanobu, Nakamura, Makoto, Sakai, Shinji

Biofacrication　Vol. 12　No. 1　p. 011001-011001　2019/08　Research paper (scientific journal)

Gelatin/hyaluronic acid content in hydrogels obtained through blue light-induced gelation affects hydrogel properties and adipose stem cell behaviors

Shinji Sakai, Hiromi Ohi, Masahito Taya

Biomolecules　Vol. 9　No. 8　2019/08　Research paper (scientific journal)

Use of Anionic Polysaccharides in the Development of 3D Bioprinting Technology

Chia Tai, Soukaina Bouissil, Enkhtuul Gantumur, Mary Stephanie Carranza, Ayano Yoshii, Shinji Sakai, Guillaume Pierre, Philippe Michaud, Cédric Delattre

Applied Sciences　Vol. 9　No. 13　p. 2596-2596　2019/06/27　Research paper (scientific journal)

Characterization of encapsulated cells within hyaluronic acid and alginate microcapsules produced via horseradish peroxidase-catalyzed crosslinking

Mehdi Khanmohammadi, Shinji Sakai, Masahito Taya

Journal of Biomaterials Science, Polymer Edition　Vol. 30　No. 4　p. 295-307　2019/03/04　Research paper (scientific journal)

Horseradish peroxidase-catalyzed hydrogelation consuming enzyme-produced hydrogen peroxide in the presence of reducing sugars

Enkhtuul Gantumur, Shinji Sakai, Masaki Nakahata, Masahito Taya

Soft Matter　Vol. 15　No. 10　p. 2163-2169　2019　Research paper (scientific journal)

Biofabrication offers future hope for tackling various obstacles and challenges in tissue engineering and regenerative medicine: A Perspective

Tanveer Ahmad Mir, Shintaroh Iwanaga, Taketoshi Kurooka, Hideki Toda, Shinji Sakai, Makoto Nakamura

International Journal of Bioprinting　Vol. 5　No. 1　p. 153-153　2018/12/31　Research paper (scientific journal)

Cross-Linking Building Blocks Using a “Boronate Bridge” to Build Functional Hybrid Materials

Masaki Nakahata, Shinji Sakai

ChemNanoMat　Vol. 5　No. 2　p. 141-151　2018/11/30　Research paper (scientific journal)

Peroxidase-catalyzed microextrusion bioprinting of cell-laden hydrogel constructs in vaporized ppm-level hydrogen peroxide

Shinji Sakai, Kei Mochizuki, Yanfei Qu, Matthew Mail, Masaki Nakahata, Masahito Taya

Biofabrication　Vol. 10　No. 4　2018/09/06　Research paper (scientific journal)

Versatility of hydrogelation by dual-enzymatic reactions with oxidases and peroxidase

Masaki Nakahata, Enkhtuul Gantumur, Kotoko Furuno, Shinji Sakai, Masahito Taya

Biochemical Engineering Journal　Vol. 131　p. 1-8　2018/03/15　Research paper (scientific journal)

Visible Light-Induced Hydrogelation of an Alginate Derivative and Application to Stereolithographic Bioprinting Using a Visible Light Projector and Acid Red

Shinji Sakai, Hidenori Kamei, Toko Mori, Tomoki Hotta, Hiromi Ohi, Masaki Nakahata, Masahito Taya

Biomacromolecules　Vol. 19　No. 2　p. 672-679　2018/02/12　Research paper (scientific journal)

Drop-On-Drop Multimaterial 3D Bioprinting Realized by Peroxidase-Mediated Cross-Linking

Shinji Sakai, Kohei Ueda, Enkhtuul Gantumur, Masahito Taya, Makoto Nakamura

Macromolecular Rapid Communications　Vol. 39　No. 3　2018/02　Research paper (scientific journal)

Differentiation potential of human adipose stem cells bioprinted with hyaluronic acid/gelatin-based bioink through microextrusion and visible light-initiated crosslinking

Shinji Sakai, Hiromi Ohi, Tomoki Hotta, Hidenori Kamei, Masahito Taya

Biopolymers　Vol. 109　No. 2　2018/02　Research paper (scientific journal)

AFOB Special Issue on Stem Cells in Tissue Engineering and Regenerative Medicine

Shinji Sakai, João F. Mano

Biotechnology Journal　Vol. 12　No. 12　2017/12/18　Research paper (scientific journal)

Horseradish Peroxidase Catalyzed Hydrogelation for Biomedical, Biopharmaceutical, and Biofabrication Applications

Shinji Sakai, Masaki Nakahata

CHEMISTRY-AN ASIAN JOURNAL　Vol. 12　No. 24　p. 3098-3109　2017/12

Fabrication of single and bundled filament-like tissues using biodegradable hyaluronic acid-based hollow hydrogel fibers

Mehdi Khanmohammadi, Shinji Sakai, Masahito Taya

International Journal of Biological Macromolecules　Vol. 104　p. 204-212　2017/11　Research paper (scientific journal)

Cytocompatible Enzymatic Hydrogelation Mediated by Glucose and Cysteine Residues

Enkhtuul Gantumur, Shinji Sakai, Masaki Nakahata, Masahito Taya

ACS Macro Letters　Vol. 6　No. 5　p. 485-488　2017/05/16　Research paper (scientific journal)

Inkjetting Plus Peroxidase-Mediated Hydrogelation Produces Cell-Laden, Cell-Sized Particles with Suitable Characters for Individual Applications

Shinji Sakai, Yusuke Yamamoto, Gantumur Enkhtuul, Kohei Ueda, Kenichi Arai, Masahito Taya, Makoto Nakamura

Macromolecular Bioscience　Vol. 17　No. 5　2017/05/01　Research paper (scientific journal)

Impact of immobilizing of low molecular weight hyaluronic acid within gelatin-based hydrogel through enzymatic reaction on behavior of enclosed endothelial cells

Mehdi Khanmohammadi, Shinji Sakai, Masahito Taya

International Journal of Biological Macromolecules　Vol. 97　p. 308-316　2017/04　Research paper (scientific journal)

Naphthalimide–coumarin conjugate: ratiometric fluorescent receptor for self-calibrating quantification of cyanide anions in cells

Yasuhiro Shiraishi, Naoto Hayashi, Masaki Nakahata, Shinji Sakai, Takayuki Hirai

RSC Advances　Vol. 7　No. 51　p. 32304-32309　2017　Research paper (scientific journal)

In vitro formation of vascular-like networks using hydrogels

Takayuki Takei, Shinji Sakai, Masahiro Yoshida

Journal of Bioscience and Bioengineering　Vol. 122　No. 5　p. 519-527　2016/11　Research paper (scientific journal)

Enzymatically-gellable galactosylated chitosan: Hydrogel characteristics and hepatic cell behavior

Ali Baradar Khoshfetrat, Mehdi Khanmohammadi, Shinji Sakai, Masahito Taya

International Journal of Biological Macromolecules　Vol. 92　p. 892-899　2016/11/01　Research paper (scientific journal)

Anchoring PEG-oleate to cell membranes stimulates reactive oxygen species production

Shinji Sakai, Koujiro Nomura, Kei Mochizuki, Masahito Taya

Colloids and Surfaces B: Biointerfaces　Vol. 147　p. 336-342　2016/11/01　Research paper (scientific journal)

Propagation of human iPS cells in alginate-based microcapsules prepared using reactions catalyzed by horseradish peroxidase and catalase

Tomoaki Ashida, Shinji Sakai, Masahito Taya

Artificial Cells, Nanomedicine and Biotechnology　Vol. 44　No. 6　p. 1406-1409　2016/08/17　Research paper (scientific journal)

Production of hyaluronic-acid-based cell-enclosing microparticles and microcapsules via enzymatic reaction using a microfluidic system

Mehdi Khanmohammadi, Shinji Sakai, Tomoaki Ashida, Masahito Taya

Journal of Applied Polymer Science　Vol. 133　No. 16　2016/04　Research paper (scientific journal)

Cryopreservation of a small number of human sperm using enzymatically fabricated, hollow hyaluronan microcapsules handled by conventional ICSI procedures

Kazuhisa Tomita, Shinji Sakai, Mehdi Khanmohammadi, Takayuki Yamochi, Shu Hashimoto, Masayuki Anzai, Yoshiharu Morimoto, Masahito Taya, Yoshihiko Hosoi

Journal of Assisted Reproduction and Genetics　Vol. 33　No. 4　p. 501-511　2016/04　Research paper (scientific journal)

Engineering tissues with a perfusable vessel-like network using endothelialized alginate hydrogel fiber and spheroid-enclosing microcapsules

Yang Liu, Shinji Sakai, Masahito Taya

Heliyon　Vol. 2　No. 2　2016/02/01　Research paper (scientific journal)

Highly efficient and low toxic skin penetrants composed of amino acid ionic liquids

Shin-ya Furukawa, Gaku Hattori, Shinji Sakai, Noriho Kamiya

RSC Advances　Vol. 6　No. 90　p. 87753-87755　2016　Research paper (scientific journal)

Automated Construction System for Cellular Tissue Based on Laminar Flow Fibers

Masaru Kojima, Akira Fukushima, Suguru Takata, Mitsuhiro Horade, Kenichi Ohara, Shinji Sakai, Kazuto Kamiyama, Yasushi Mae, Tatsuo Arai

2016 IEEE INTERNATIONAL CONFERENCE ON MECHATRONICS AND AUTOMATION　p. 920-925　2016　Research paper (international conference proceedings)

The development of cell-adhesive hydrogel for 3D printing

Kenichi Arai, Yoshinari Tsukamoto, Hirotoshi Yoshida, Hidetoshi Sanae, Tanveer Ahmad Mir, Shinji Sakai, Toshiko Yoshida, Motonori Okabe, Toshio Nikaido, Masahito Taya, Makoto Nakamura

International Journal of Bioprinting　Vol. 2　No. 2　p. 153-162　2016　Research paper (scientific journal)

Electrochemical recycling of gold nanofibrous membrane as an enzyme immobilizing carrier

Koichi Sawada, Shinji Sakai, Masahito Taya

Chemical Engineering Journal　Vol. 280　p. 558-563　2015/11/05　Research paper (scientific journal)

Peritoneal adhesion prevention by a biodegradable hyaluronic acid-based hydrogel formed in situ through a cascade enzyme reaction initiated by contact with body fluid on tissue surfaces

Shinji Sakai, Kohei Ueda, Masahito Taya

Acta Biomaterialia　Vol. 24　p. 152-158　2015/09/15　Research paper (scientific journal)

Characteristics of duplex microcapsules prepared from an alginate-derivative polymer via horseradish peroxidase- and catalase-catalyzed reactions

Tomoaki Ashida, Shinji Sakai, Masahito Taya

Journal of Chemical Engineering of Japan　Vol. 48　No. 7　p. 588-591　2015/07/20　Research paper (scientific journal)

Cancer stem cell marker-expressing cell-rich spheroid fabrication from PANC-1 cells using alginate microcapsules with spherical cavities templated by gelatin microparticles

Shinji Sakai, Kazuya Inamoto, Tomoaki Ashida, Ryo Takamura, Masahito Taya

Biotechnology Progress　Vol. 31　No. 4　p. 1071-1076　2015/07/01　Research paper (scientific journal)

Cell-selective encapsulation in hydrogel sheaths via biospecific identification and biochemical cross-linking

Shinji Sakai, Yang Liu, Mikako Sengoku, Masahito Taya

Biomaterials　Vol. 53　p. 494-501　2015/06/01　Research paper (scientific journal)

Identification of hydrogen peroxide-secreting cells by cytocompatible coating with a hydrogel membrane

Yang Liu, Shinji Sakai, Shogo Kawa, Masahito Taya

Analytical Chemistry　Vol. 86　No. 23　p. 11592-11598　2014/12/02　Research paper (scientific journal)

On-cell surface cross-linking of polymer molecules by horseradish peroxidase anchored to cell membrane for individual cell encapsulation in hydrogel sheath

Shinji Sakai, Masahito Taya

ACS Macro Letters　Vol. 3　No. 10　p. 972-975　2014/10/21　Research paper (scientific journal)

Horseradish peroxidase-catalyzed formation of hydrogels from chitosan and poly(vinyl alcohol) derivatives both possessing phenolic hydroxyl groups

Shinji Sakai, Mehdi Khanmohammadi, Ali Baradar Khoshfetrat, Masahito Taya

Carbohydrate Polymers　Vol. 111　p. 404-409　2014/10/13　Research paper (scientific journal)

Polyacrylonitrile-based electrospun nanofibers carrying gold nanoparticles in situ formed by photochemical assembly

Koichi Sawada, Shinji Sakai, Masahito Taya

Journal of Materials Science　Vol. 49　No. 13　p. 4595-4600　2014/07　Research paper (scientific journal)

Horseradish peroxidase-mediated encapsulation of mammalian cells in hydrogel particles by dropping

Shinji Sakai, Tomoaki Ashida, Shotaro Ogino, Masahito Taya

Journal of Microencapsulation　Vol. 31　No. 1　p. 100-104　2014　Research paper (scientific journal)

Fabrication of ultrafine carbon fibers possessing a nanoporous structure from electrospun polyvinyl alcohol fibers containing silica nanoparticles

Koichi Sawada, Shinji Sakai, Masahito Taya

Journal of Nanomaterials　Vol. 2014　2014　Research paper (scientific journal)

Automated Cell Stiffness Measurement with Two-Fingered Microhand using Micro Force Sensor

Hiroyuki Yabugaki, Kenichi Ohara, Masaru Kojima, Mitsuhiro Horade, Kazuto Kamiyama, Shinji Sakai, Takanori Kihara, Yasushi Mae, Tamio Tanikawa, Tatsuo Arai

2014 IEEE/ASME INTERNATIONAL CONFERENCE ON ADVANCED INTELLIGENT MECHATRONICS (AIM)　p. 384-389　2014　Research paper (international conference proceedings)

Development of Hydrogel Coated End Effector for Stable Cell Manipulation by Two-fingered Micro-hand

Masaru Kojima, Hiroyuki Yabugaki, Ebubekir Avci, Kenichi Ohara, Shinji Sakai, Mitsuhiro Horade, Kazuto Kamiyama, Yasushi Mae, Tatsuo Arai

2014 IEEE INTERNATIONAL CONFERENCE ON MECHATRONICS AND AUTOMATION (IEEE ICMA 2014)　p. 43-48　2014　Research paper (international conference proceedings)

Designing fusion proteins with carbohydrate-binding modules having affinity to enzymatically gellable carboxymethylcellulose derivative hydrogel

Tomoaki Ashida, Yoshihiro Ojima, Shinji Sakai, Makiko Sakka, Kazuo Sakka, Koei Kawakami, Masahito Taya

Journal of Chemical Engineering of Japan　Vol. 47　No. 11　p. 835-840　2014　Research paper (scientific journal)

Cell Stiffness Measurement Using Two-fingered Micro-hand Equipped with Plate-shaped End Effector

Nyamdorj Khangai, Masaru Kojima, Mitsuhiro Horade, Kazuto Kamiyama, Shinji Sakai, Yasushi Mae, Tatsuo Arai

2014 11TH INTERNATIONAL CONFERENCE ON UBIQUITOUS ROBOTS AND AMBIENT INTELLIGENCE (URAI)　p. 517-521　2014　Research paper (international conference proceedings)

Competing two enzymatic reactions realizing one-step preparation of cell-enclosing duplex microcapsules

Tomoaki Ashida, Shinji Sakai, Masahito Taya

Biotechnology Progress　Vol. 29　No. 6　p. 1528-1534　2013/11　Research paper (scientific journal)

Polyvinyl alcohol-based hydrogel dressing gellable on-wound via a co-enzymatic reaction triggered by glucose in the wound exudate

Shinji Sakai, Mika Tsumura, Mitsugu Inoue, Yukinori Koga, Kenji Fukano, Masahito Taya

Journal of Materials Chemistry B　Vol. 1　No. 38　p. 5067-5075　2013/10/14　Research paper (scientific journal)

Fabrication of in vitro three-dimensional multilayered blood vessel model using human endothelial and smooth muscle cells and high-strength PEG hydrogel

Sho Shinohara, Takanori Kihara, Shinji Sakai, Michiya Matsusaki, Mitsuru Akashi, Masahito Taya, Jun Miyake

Journal of Bioscience and Bioengineering　Vol. 116　No. 2　p. 231-234　2013/08　Research paper (scientific journal)

Enzymatically-gelled amylopectin-based substrates enable on-demand harvesting cells with preserving cell-to-cell connection using saliva

Shinji Sakai, Yang Liu, Masahito Taya

Journal of Bioscience and Bioengineering　Vol. 115　No. 4　p. 462-465　2013/04　Research paper (scientific journal)

Electrospun polystyrene fiber-templating ultrafine gold hollow fiber production

Shinji Sakai, Shogo Kawa, Koichi Sawada, Masahito Taya

Gold Bulletin　Vol. 46　No. 2　p. 97-101　2013/03/09　Research paper (scientific journal)

Horseradish peroxidase/catalase-mediated cell-laden alginate-based hydrogel tube production in two-phase coaxial flow of aqueous solutions for filament-like tissues fabrication

Shinji Sakai, Yang Liu, Emma Jane Mah, Masahito Taya

Biofabrication　Vol. 5　No. 1　2013/03　Research paper (scientific journal)

Impact of the composition of alginate and gelatin derivatives in bioconjugated hydrogels on the fabrication of cell sheets and spherical tissues with living cell sheaths

Yang Liu, Shinji Sakai, Masahito Taya

Acta Biomaterialia　Vol. 9　No. 5　p. 6616-6623　2013　Research paper (scientific journal)

Bone Regeneration of Tibial Defects in Rats with Enzymatic Hydrogelation of Gelatin Derivative and Recombinant Human Platelet-Derived Growth Factor-BB Complex

Daisuke Kondo, Yoichiro Ogino, Yasunori Ayukawa, Shinji Sakai, Koei Kawakami, Kiyoshi Koyano

The International Journal of Oral & Maxillofacial Implants　Vol. 28　No. 5　p. 1377-1385　2013　Research paper (scientific journal)

Rapidly serum-degradable hydrogel templating fabrication of spherical tissues and curved tubular structures

Shinji Sakai, Hitomi Inagaki, Yang Liu, Tomohiro Matsuyama, Takanori Kihara, Jun Miyake, Koei Kawakami, Masahito Taya

Biotechnology and Bioengineering　Vol. 109　No. 11　p. 2911-2919　2012/11　Research paper (scientific journal)

An electrospun ultrafine fibrous silica catalyst incorporating an alkyl-silica coating containing lipase for reactions in organic solvents

Shinji Sakai, Yuping Liu, Rie Watanabe, Masaaki Kawabe, Masahito Taya, Koei Kawakami

Journal of Molecular Catalysis B: Enzymatic　Vol. 83　p. 120-124　2012/11　Research paper (scientific journal)

Wrapping tissues with a pre-established cage-like layer composed of living cells

Shinji Sakai, Hitomi Inagaki, Kazuya Inamoto, Masahito Taya

Biomaterials　Vol. 33　No. 28　p. 6721-6727　2012/10　Research paper (scientific journal)

Production of endothelial cell-enclosing alginate-based hydrogel fibers with a cell adhesive surface through simultaneous cross-linking by horseradish peroxidase-catalyzed reaction in a hydrodynamic spinning process

Yang Liu, Shinji Sakai, Masahito Taya

Journal of Bioscience and Bioengineering　Vol. 114　No. 3　p. 353-359　2012/09　Research paper (scientific journal)

Enhanced productivity of electrospun polyvinyl alcohol nanofibrous mats using aqueous N,N-dimethylformamide solution and their application to lipase-immobilizing membrane-shaped catalysts

Koichi Sawada, Shinji Sakai, Masahito Taya

Journal of Bioscience and Bioengineering　Vol. 114　No. 2　p. 204-208　2012/08　Research paper (scientific journal)

Adipose tissue engineering using adipose-derived stem cells enclosed within an injectable carboxymethylcellulose-based hydrogel

Yuko Ogushi, Shinji Sakai, Koei Kawakami

Journal of Tissue Engineering and Regenerative Medicine　Vol. 7　No. 11　p. 884-892　2012/04/04　Research paper (scientific journal)

Glucose-triggered co-enzymatic hydrogelation of aqueous polymer solutions

Shinji Sakai, Kimiko Komatani, Masahito Taya

RSC Advances　Vol. 2　No. 4　p. 1502-1507　2012/02/21　Research paper (scientific journal)

On-demand serum-degradable amylopectin-based in situ gellable hydrogel

Shinji Sakai, Yang Liu, Tomohiro Matsuyama, Koei Kawakami, Masahito Taya

Journal of Materials Chemistry　Vol. 22　No. 5　p. 1944-1949　2012/02/07　Research paper (scientific journal)

Controlling apatite microparticles formation by calcining electrospun sol-gel derived ultrafine silica fibers

Shinji Sakai, Tetsu Yamaguchi, Ryan Anugrah Putra, Rie Watanabe, Masaaki Kawabe, Masahito Taya, Koei Kawakami

Journal of Sol-Gel Science and Technology　Vol. 61　No. 2　p. 374-380　2012/02　Research paper (scientific journal)

Multicellular tumor spheroid formation in duplex microcapsules for analysis of chemosensitivity

Shinji Sakai, Kazuya Inamoto, Yang Liu, Shinji Tanaka, Shigeki Arii, Masahito Taya

Cancer Science　Vol. 103　No. 3　p. 549-554　2012/01/13　Research paper (scientific journal)

Controlling the diameters of silica nanofibers obtained by sol-gel/electrospinning methods

Shinji Sakai, Koei Kawakami, Masahito Taya

Journal of Chemical Engineering of Japan　Vol. 45　No. 6　p. 436-440　2012　Research paper (scientific journal)

Cell-enclosing gelatin-based microcapsule production for tissue engineering using a microfluidic flow-focusing system

Shinji Sakai, Sho Ito, Hitomi Inagaki, Keisuke Hirose, Tomohiro Matsuyama, Masahito Taya, Koei Kawakami

Biomicrofluidics　Vol. 5　No. 1　2011/03/01　Research paper (scientific journal)

Controlling Thermo-Reversibility of Gelatin Gels through a Peroxidase-Catalyzed Reaction under Mild Conditions for Mammalian Cells

Shinji Sakai, Kousuke Moriyama, Koei Kawakami

JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION　Vol. 22　No. 9　p. 1147-1156　2011　Research paper (scientific journal)

Development of Porous Alginate-Based Scaffolds Covalently Cross-Linked through a Peroxidase-Catalyzed Reaction

Shinji Sakai, Koei Kawakami

JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION　Vol. 22　No. 18　p. 2407-2416　2011　Research paper (scientific journal)

Hepatocytes exhibit constant metabolic activity on carboxymethylcellulose-based hydrogel with high phenolic hydroxy group content

Yuko Ogushi, Shinji Sakai, Koei Kawakami

Biochemical Engineering Journal　Vol. 51　No. 3　p. 147-152　2010/09　Research paper (scientific journal)

Calcium alginate microcapsules with spherical liquid cores templated by gelatin microparticles for mass production of multicellular spheroids

Shinji Sakai, Sho Ito, Koei Kawakami

ACTA BIOMATERIALIA　Vol. 6　No. 8　p. 3132-3137　2010/08　Research paper (scientific journal)

Immobilization of Pseudomonas cepacia lipase onto electrospun polyacrylonitrile fibers through physical adsorption and application to transesterification in nonaqueous solvent

Shinji Sakai, Yuping Liu, Tetsu Yamaguchi, Rie Watanabe, Masaaki Kawabe, Koei Kawakami

BIOTECHNOLOGY LETTERS　Vol. 32　No. 8　p. 1059-1062　2010/08　Research paper (scientific journal)

Effect of a hepatocyte growth factor/heparin-immobilized collagen system on albumin synthesis and spheroid formation by hepatocytes

Yung-Te Hou, Hiroyuki Ijima, Shunichi Matsumoto, Takafumi Kubo, Takayuki Takei, Shinji Sakai, Koei Kawakami

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　Vol. 110　No. 2　p. 208-216　2010/08　Research paper (scientific journal)

Hematin is an Alternative Catalyst to Horseradish Peroxidase for In Situ Hydrogelation of Polymers with Phenolic Hydroxyl Groups In Vivo

Shinji Sakai, Kousuke Moriyama, Kenichi Taguchi, Koei Kawakami

BIOMACROMOLECULES　Vol. 11　No. 8　p. 2179-2183　2010/08　Research paper (scientific journal)

Preparation of cell-enclosing microcapsules through photopolymerization of methacrylated alginate solution triggered by irradiation with visible light

Changjun Mu, Shinji Sakai, Hiroyuki Ijima, Koei Kawakami

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　Vol. 109　No. 6　p. 618-621　2010/06　Research paper (scientific journal)

In Situ Simultaneous Protein-Polysaccharide Bioconjugation and Hydrogelation Using Horseradish Peroxidase

Shinji Sakai, Tomohiro Matsuyama, Keisuke Hirose, Koei Kawakami

BIOMACROMOLECULES　Vol. 11　No. 5　p. 1370-1375　2010/05　Research paper (scientific journal)

Control of cellular adhesiveness in an alginate-based hydrogel by varying peroxidase and H2O2 concentrations during gelation

S. Sakai, K. Hirose, K. Moriyama, K. Kawakami

ACTA BIOMATERIALIA　Vol. 6　No. 4　p. 1446-1452　2010/04　Research paper (scientific journal)

Electrospun PVA fibrous mats immobilizing lipase entrapped in alkylsilicate cages: Application to continuous production of fatty acid butyl ester

Shinji Sakai, Koichi Antoku, Tetsu Yamaguchi, Rie Watanable, Masaaki Kawabe, Koei Kawakami

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC　Vol. 63　No. 1-2　p. 57-61　2010/04　Research paper (scientific journal)

Heat treatment of electrospun silicate fiber substrates enhances cellular adhesion and proliferation

Tetsu Yamaguchi, Shinji Sakai, Rie Watanabe, Takashi Tarao, Koei Kawakami

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　Vol. 109　No. 3　p. 304-306　2010/03　Research paper (scientific journal)

Novel technique to control inner and outer diameter of calcium-alginate hydrogel hollow microfibers, and immobilization of mammalian cells

Takayuki Takei, Naoya Kishihara, Shinji Sakai, Koei Kawakami

BIOCHEMICAL ENGINEERING JOURNAL　Vol. 49　No. 1　p. 143-147　2010/03　Research paper (scientific journal)

Enhanced catalytic activity of lipase in situ encapsulated in electrospun polystyrene fibers by subsequent water supply

Shinji Sakai, Tetsu Yamaguchi, Rie Watanabe, Masaaki Kawabe, Koei Kawakami

CATALYSIS COMMUNICATIONS　Vol. 11　No. 6　p. 576-580　2010/02　Research paper (scientific journal)

酵素による架橋と分解を利用する材料設計と再生医療用技術への適用

境 慎司, 川上 幸衛, 田谷 正仁

化学工学会 研究発表講演要旨集　Vol. 2010　p. 562-562　2010

三次元生体組織構築のための微少流路作製法の開発

岸原 尚也, 武井 孝行, 境 慎司, 井嶋 博之, 川上 幸衛

化学工学会 研究発表講演要旨集　Vol. 2010　p. 955-955　2010

肝組織工学のための細胞包埋ゲル充填scaffold培養基材の開発

井嶋 博之, 久保 孝文, 白木川 奈菜, 侯 詠徳, 武井 孝行, 境 慎司, 川上 幸衛

化学工学会 研究発表講演要旨集　Vol. 2010　No. 0　p. 121-121　2010

Development of Subsieve-Size Capsules and Application to Cell Therapy

Shinji Sakai, Koei Kawakami

THERAPEUTIC APPLICATIONS OF CELL MICROENCAPSULATION　Vol. 670　p. 22-30　2010　Research paper (scientific journal)

Enzymatically fabricated and degradable microcapsules for production of multicellular spheroids with well-defined diameters of less than 150 mu m

Shinji Sakai, Sho Ito, Yuko Ogushi, Ichiro Hashimoto, Natsuko Hosoda, Yoshinori Sawae, Koei Kawakami

BIOMATERIALS　Vol. 30　No. 30　p. 5937-5942　2009/10　Research paper (scientific journal)

細胞包埋ゲル充填多孔質scaffold培養技術の開発と肝組織工学に向けた基礎的検討

井嶋 博之, 侯 詠徳, 久保 孝文, 白木川 奈菜, 武井 孝行, 境 慎司, 川上 幸衛

日本生物工学会大会講演要旨集　Vol. 平成21年度　p. 146-146　2009/08

An injectable, in situ enzymatically gellable, gelatin derivative for drug delivery and tissue engineering

Shinji Sakai, Keisuke Hirose, Kenichi Taguchi, Yuko Ogushi, Koei Kawakami

BIOMATERIALS　Vol. 30　No. 20　p. 3371-3377　2009/07　Research paper (scientific journal)

Application of a lipase-immobilized silica monolith bioreactor to the production of fatty acid methyl esters

Koei Kawakami, Ryo Takahashi, Mozaffar Shakeri, Shinji Sakai

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC　Vol. 57　No. 1-4　p. 194-197　2009/05　Research paper (scientific journal)

Phenolic Hydroxy Groups Incorporated for the Peroxidase-Catalyzed Gelation of a Carboxymethylcellulose Support: Cellular Adhesion and Proliferation

Yuko Ogushi, Shinji Sakai, Koei Kawakami

MACROMOLECULAR BIOSCIENCE　Vol. 9　No. 3　p. 262-267　2009/03　Research paper (scientific journal)

Surface immobilization of poly(ethyleneimine) and plasmid DNA on electrospun poly(L-lactic acid) fibrous mats using a layer-by-layer approach for gene delivery

Shinji Sakai, Yusuke Yamada, Tetsu Yamaguchi, Tomasz Ciach, Koei Kawakami

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A　Vol. 88A　No. 2　p. 281-287　2009/02　Research paper (scientific journal)

Enzymatically crosslinked carboxymethylcellulose-tyramine conjugate hydrogel: Cellular adhesiveness and feasibility for cell sheet technology

Shinji Sakai, Yuko Ogushi, Koei Kawakami

ACTA BIOMATERIALIA　Vol. 5　No. 2　p. 554-559　2009/02　Research paper (scientific journal)

ゼラチン-チラミンコンジュゲートの作製とその評価

境 慎司, 廣瀬 圭介, 大串 裕子, 川上 幸衛

化学工学会 研究発表講演要旨集　Vol. 2009　p. 730-730　2009

酵素架橋性カルボキシメチルセルロースゲルの再生医療への応用

大串 裕子, 境 慎司, 武井 孝行, 井嶋 博之, 川上 幸衛

化学工学会 研究発表講演要旨集　Vol. 2009　p. 731-731　2009

リパーゼ固定化シリカモノリスバイオリアクターの特性評価およびバイオディーゼル生産への応用

高橋 涼, 武井 孝行, 境 慎司, 井嶋 博之, 川上 幸衛

化学工学会 研究発表講演要旨集　Vol. 2009　p. 645-645　2009

ナノファイバー細胞培養担体を用いた還流培養システムの構築

山口 哲, 境 慎司, 渡邊 理恵, 多羅尾 隆, 川上 幸衛

化学工学会 研究発表講演要旨集　Vol. 2009　p. 665-665　2009

機能性肝細胞培養技術の構築と肝組織工学に向けたプロセス開発

井嶋 博之, 侯 詠徳, 久保 孝文, 松本 峻一, 武井 孝行, 境 慎司, 川上 幸衛

化学工学会 研究発表講演要旨集　Vol. 2009　p. 918-918　2009

マイクロカプセルを利用した球状組織体の調製

伊藤 翔, 武井 孝行, 境 慎司, 井嶋 博之, 川上 幸衛

化学工学会 研究発表講演要旨集　Vol. 2009　p. 1047-1047　2009

トランスグルタミナーゼによる機能性分子固定化機構を有する再生医療技術の開発

久保 孝文, 井嶋 博之, 神谷 典穂, 河邉 佳典, 武井 孝行, 境 慎司, 上平 正道, 川上 幸衛

化学工学会 研究発表講演要旨集　Vol. 2009　p. 1039-1039　2009

Small Agarose Microcapsules With Cell-Enclosing Hollow Core for Cell Therapy: Transplantation of Ifosfamide-Activating Cells to the Mice With Preestablished Subcutaneous Tumor

Shinji Sakai, Ichiro Hashimoto, Shinji Tanaka, Brian Salmons, Koei Kawakami

CELL TRANSPLANTATION　Vol. 18　No. 8　p. 933-939　2009　Research paper (scientific journal)

Novel chitosan derivative soluble at neutral pH and in-situ gellable via peroxidase-catalyzed enzymatic reaction

Shinji Sakai, Yusuke Yamada, Takashi Zenke, Koei Kawakami

JOURNAL OF MATERIALS CHEMISTRY　Vol. 19　No. 2　p. 230-235　2009　Research paper (scientific journal)

Application of silicate electrospun nanofibers for cell culture

Tetsu Yamaguchi, Shinji Sakai, Koei Kawakami

JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY　Vol. 48　No. 3　p. 350-355　2008/12　Research paper (scientific journal)

Development of electrospun poly(vinyl alcohol) fibers immobilizing lipase highly activated by alkyl-silicate for flow-through reactors

Shinji Sakai, Koichi Antoku, Tetsu Yamaguchi, Koei Kawakami

JOURNAL OF MEMBRANE SCIENCE　Vol. 325　No. 1　p. 454-459　2008/11　Research paper (scientific journal)

Feasibility of carboxymethylcellulose with phenol moieties as a material for mammalian cell-enclosing subsieve-size capsules

Shinji Sakai, Sho Ito, Yuko Ogushi, Ichiro Hashimoto, Koei Kawakami

CELLULOSE　Vol. 15　No. 5　p. 723-729　2008/10　Research paper (scientific journal)

Reinforcement of porous alginate scaffolds by incorporating electrospun fibres

Shinji Sakai, Yousuke Takagi, Yusuke Yamada, Tetsu Yamaguchi, Koei Kawakami

BIOMEDICAL MATERIALS　Vol. 3　No. 3　2008/09　Research paper (scientific journal)

Oxidized alginate-cross-linked alginate/gelatin hydrogel fibers for fabricating tubular constructs with layered smooth muscle cells and endothelial cells in collagen gels

Shinji Sakai, Shinsuke Yamaguchi, Takayuki Takei, Koei Kawakami

BIOMACROMOLECULES　Vol. 9　No. 7　p. 2036-2041　2008/07　Research paper (scientific journal)

L-carnitine supplementation decreases the left ventricular mass in patients undergoing hemodialysis

Tai Sakurabayashi, Shigeru Miyazaki, Yasuko Yuasa, Shinji Sakai, Masashi Suzuki, Sachio Takahashi, Yoshihei Hirasawa

CIRCULATION JOURNAL　Vol. 72　No. 6　p. 926-931　2008/06　Research paper (scientific journal)

Transesterification by lipase entrapped in electrospun poly(vinyl alcohol) fibers and its application to a flow-through reactor

Shinji Sakai, Koichi Antoku, Tetsu Yamaguchi, Koel Kawakami

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　Vol. 105　No. 6　p. 687-689　2008/06　Research paper (scientific journal)

Both ionically and enzymatically crosslinkable alginate-tyramine conjugate as materials for cell encapsulation

Shinji Sakai, Koei Kawakami

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A　Vol. 85A　No. 2　p. 345-351　2008/05　Research paper (scientific journal)

Enhancement of enclosed-cells growth by cell-adhesive microcapsule membrane

Shinji Sakai, Ichiro Hashimoto, Koei Kawakami

8th World Biomaterials Congress 2008　Vol. 4　2008　Research paper (international conference proceedings)

カルボキシメチルセルロースへのフェノール性水酸基導入率と得られるゲルの諸特性の相関

大串 裕子, 境 慎司, 井嶋 博之, 川上 幸衛

化学工学会 研究発表講演要旨集　Vol. 2008　p. 423-423　2008

シリカ修飾リパーゼ固定化エレクトロスピニング不織布の開発

安徳 浩一, 境 慎司, 山口 哲, 井嶋 博之, 川上 幸衛

化学工学会 研究発表講演要旨集　Vol. 2008　p. 342-342　2008

可視光照射による細胞包括サブシーブカプセル作製用化学修飾アルギン酸の合成と機能評価

牟 倡駿, 境 慎司, 井嶋 博之, 川上 幸衛

化学工学会 研究発表講演要旨集　Vol. 2008　p. 606-606　2008

[Comparison of basic imaging properties for digital radiographic systems based on a direct conversion type of flat panel detector].

Sakai S, Katayama R, Morishita J, Sakaguchi T, Ohkubo S, Maeda T, Hayabuchi N

Nihon Hoshasen Gijutsu Gakkai zasshi　Vol. 64　No. 1　p. 41-49　2008/01

[Changes in the prognosis for survival of patients with end-stage renal disease (ESRD) treated by hemodialysis].

Sakurabayashi T, Kikuchi H, Nakayama H, Osawa Y, Shimada H, Miyazaki S, Yuasa Y, Sakai S, Suzuki M, Takahashi S, Hirasawa Y

Nihon Jinzo Gakkai shi　Vol. 50　No. 2　p. 127-134　2008

Agarose-gelatin conjugate membrane enhances proliferation of adherent cells enclosed in hollow-core microcapsules

Shinji Sakai, Ichiro Hashimoto, Koei Kawakami

JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION　Vol. 19　No. 7　p. 937-944　2008　Research paper (scientific journal)

Production of cell-enclosing hollow-core agarose microcapsules via jetting in water-immiscible liquid paraffin and formation of embryoid body-like spherical tissues from mouse ES cells enclosed within these microcapsules

Shinji Sakai, Ichiro Hashimoto, Koei Kawakami

BIOTECHNOLOGY AND BIOENGINEERING　Vol. 99　No. 1　p. 235-243　2008/01　Research paper (scientific journal)

Novel technique for fabricating double-layered tubular constructs consisting of two vascular cell types in collagen gels used as templates for three-dimensional tissues

Takayuki Takei, Shinsuke Yamaguchi, Shinji Sakai, Hiroyuki Ijima, Koei Kawakami

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　Vol. 104　No. 5　p. 435-438　2007/11　Research paper (scientific journal)

Development of a silica monolith microbloreactor entrapping highly activated lipase and an experiment toward integration with chromatographic separation of chiral esters

Koei Kawakami, Daisuke Abe, Taiki Urakawa, Ayako Kawashima, Yasuhiro Oda, Ryo Takahashi, Shinji Sakai

JOURNAL OF SEPARATION SCIENCE　Vol. 30　No. 17　p. 3077-3084　2007/11　Research paper (scientific journal)

Peroxidase-catalyzed cell encapsulation in subsieve-size capsules of alginate with phenol moieties in water-immiscible fluid dissolving H2O2

Shinji Sakai, Ichiro Hashimoto, Yuko Ogushi, Koei Kawakami

BIOMACROMOLECULES　Vol. 8　No. 8　p. 2622-2626　2007/08　Research paper (scientific journal)

Synthesis and characterization of both ionically and enzymatically cross-linkable alginate

Shinji Sakai, Koel Kawakami

ACTA BIOMATERIALIA　Vol. 3　No. 4　p. 495-501　2007/07　Research paper (scientific journal)

[Effect on image data resampling in evaluation of the basic imaging properties for a digital radiographic system based on a flat panel detector].

Katayama R, Sakai S, Morishita J, Sakaguchi T, Ohkubo S, Maeda T, Takada K, Hayabuchi N

Nihon Hoshasen Gijutsu Gakkai zasshi　Vol. 63　No. 7　p. 749-756　2007/07

Synthesis of enzymatically-gellable carboxymethylcellulose for biomedical applications

Yuko Ogushi, Shinji Sakai, Koei Kawakami

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　Vol. 104　No. 1　p. 30-33　2007/07　Research paper (scientific journal)

Agarose-gelatin conjugate for adherent cell-enclosing capsules

Shinji Sakai, Ichiro Hashimoto, Koei Kawakami

BIOTECHNOLOGY LETTERS　Vol. 29　No. 5　p. 731-735　2007/05　Research paper (scientific journal)

Laccase-mediated degradation and reduction of toxicity of the postharvest fungicide imazalil

Tatsuo Maruyama, Chiho Komatsu, Junji Michizoe, Shinji Sakai, Masahiro Goto

PROCESS BIOCHEMISTRY　Vol. 42　No. 3　p. 459-461　2007/03　Research paper (scientific journal)

Expression of a liver-specific function by a hepatoblastoma cell line cocultured with three-dimensional endothelialized tubes in collagen gels

Takayuki Takei, Shinji Sakai, Hiroyuki Ijima, Koei Kawakami

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　Vol. 103　No. 2　p. 200-202　2007/02　Research paper (scientific journal)

初代ラット肝細胞のコラーゲンゲル内共培養系の構築とその肝機能及び組織学的評価

掛谷 泰雄, 横沼 徹, 境 慎司, 井嶋 博之, 川上 幸衛

化学工学会 研究発表講演要旨集　Vol. 2007　p. 714-714　2007

コラーゲンゲル内における血管様構造体の構築

山口 晋輔, 武井 孝行, 境 慎司, 井嶋 博之, 川上 幸衛

化学工学会 研究発表講演要旨集　Vol. 2007　p. 992-992　2007

フェノール基含有カルボキシメチルセルロースゲルの作製と評価

大串 裕子, 境 慎司, 井嶋 博之, 川上 幸衛

化学工学会 研究発表講演要旨集　Vol. 2007　p. 359-359　2007

エレクトロスピニング法を用いたファイバーの遺伝子導入担体としての有用性評価

山田 裕介, 山口 哲, 境 慎司, 井嶋 博之, 川上 幸衛

化学工学会 研究発表講演要旨集　Vol. 2007　p. 416-416　2007

初代ラット肝細胞の薬物代謝酵素活性に及ぼす培養環境の影響

掛谷 泰雄, 松尾 健, 境 慎司, 井嶋 博之, 川上 幸衛

化学工学会 研究発表講演要旨集　Vol. 2007　p. 384-384　2007

Fabrication of artificial endothelialized tubes with predetermined three-dimensional configuration from flexible cell-enclosing alginate fibers

Takayuki Takei, Shinji Sakai, Toru Yokonuma, Hiroyuki Ijima, Koei Kawakami

BIOTECHNOLOGY PROGRESS　Vol. 23　No. 1　p. 182-186　2007/01　Research paper (scientific journal)

Synthesis of an agarose-gelatin conjugate for use as a tissue engineering scaffold

Shinji Sakai, Ichiro Hashimoto, Koei Kawakami

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　Vol. 103　No. 1　p. 22-26　2007/01　Research paper (scientific journal)

Effects of lipid composition on entrapment of proteins into phosphatidylglycerol-containing liposomes

Sunao Murakami, Tetsuya Fujita, Tsutomu Ono, Shinji Sakai, Hiroyuki Ijima, Koei Kawakami

KAGAKU KOGAKU RONBUNSHU　Vol. 32　No. 6　p. 514-517　2006/11　Research paper (scientific journal)

2005 Japanese Society for Dialysis Therapy guidelines for vascular access construction and repair for chronic hemodialysis.

Ohira S, Naito H, Amano I, Azuma N, Ikeda K, Kukita K, Goto Y, Sakai S, Shinzato T, Sugimoto T, Takemoto Y, Haruguchi H, Hino I, Hiranaka T, Mizuguchi J, Miyata A, Murotani N, Japanese Society for Dialysis Therapy

Therapeutic apheresis and dialysis : official peer-reviewed journal of the International Society for Apheresis, the Japanese Society for Apheresis, the Japanese Society for Dialysis Therapy　Vol. 10　No. 5　p. 449-462　2006/10

Prospective use of electrospun ultra-fine silicate fibers for bone tissue engineering

Sakai, Shinji, Yamada, Yusuke, Yamaguchi, Tetsu, Kawakami, Koei

Biotechnol J　Vol. 1　No. 9　p. 958-962　2006/09　Research paper (scientific journal)

Prospective use of electrospun ultra-fine silicate fibers for bone tissue engineering

Shinji Sakai, Yusuke Yamada, Tetsu Yamaguchi, Koei Kawakami

Biotechnology Journal　Vol. 1　No. 9　p. 958-962　2006/09　Research paper (scientific journal)

Development of mammalian cell-enclosing calcium-alginate hydrogel fibers in a co-flowing stream

Takayuki Takei, Shinji Sakai, Hiroyuki Ijima, Koei Kawakami

Biotechnology Journal　Vol. 1　No. 9　p. 1014-1017　2006/09　Research paper (scientific journal)

Fabrication of endothelialized tube in collagen gel as starting point for self-developing capillary-like network to construct three-dimensional organs in vitro

Takayuki Takei, Shinji Sakai, Tsutomu Ono, Hiroyuki Ijima, Koei Kawakami

BIOTECHNOLOGY AND BIOENGINEERING　Vol. 95　No. 1　p. 1-7　2006/09　Research paper (scientific journal)

Biocompatibility of subsieve-size capsules versus conventional-size microcapsules

Shinji Sakai, Changjun Mu, Kenji Kawabata, Ichiro Hashimoto, Koei Kawakami

Journal of Biomedical Materials Research - Part A　Vol. 78　No. 2　p. 394-398　2006/08　Research paper (scientific journal)

Biocompatibility of subsieve-size capsules versus conventional-size microcapsules

Shinji Sakai, Changjun Mu, Kenji Kawabata, Ichiro Hashimoto, Koei Kawakami

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A　Vol. 78A　No. 2　p. 394-398　2006/08　Research paper (scientific journal)

Usefulness of flow focusing technology for producing subsieve-size cell enclosing capsules: Application for agarose capsules production

Shinji Sakai, Ichiro Hashimoto, Koei Kawakami

BIOCHEMICAL ENGINEERING JOURNAL　Vol. 30　No. 2　p. 218-221　2006/06　Research paper (scientific journal)

Effect of diglucosamine on the entrapment of protein into liposomes

S Murakami, T Ono, S Sakai, H Ijima, K Kawakami

JOURNAL OF LIPOSOME RESEARCH　Vol. 16　No. 2　p. 103-112　2006/05　Research paper (scientific journal)

Development of alginate-agarose subsieve-size capsules for subsequent modification with a polyelectrolyte complex membrane

S Sakai, Hashimoto, I, K Kawakami

BIOCHEMICAL ENGINEERING JOURNAL　Vol. 30　No. 1　p. 76-81　2006/05　Research paper (scientific journal)

遺伝子包括ベシクルの細胞導入効率に関する検討

藤田 哲也, 小野 努, 境 慎司, 井嶋 博之, 川上 幸衛

化学工学会 研究発表講演要旨集　Vol. 2006　p. 994-994　2006

二段階ゾル‐ゲル法によるリパーゼ固定化モノリスマイクロリアクターの調製と特性

浦川 大樹, 安倍 大輔, 境 慎司, 井嶋 博之, 川上 幸衛

化学工学会 研究発表講演要旨集　Vol. 2006　p. 952-952　2006

生体血管と類似の構造を有する血管様構造体の作製

山口 晋輔, 武井 孝行, 境 慎司, 井嶋 博之, 川上 幸衛

化学工学会 研究発表講演要旨集　Vol. 2006　p. 954-954　2006

Subsieve-size agarose capsules enclosing ifosfamide-activating cells: a strategy toward chemotherapeutic targeting to tumors.

Sakai S, Kawabata K, Tanaka S, Harimoto N, Hashimoto I, Mu C, Salmons B, Ijima H, Kawakami K

Molecular cancer therapeutics　Vol. 4　No. 11　p. 1786-1790　2005/11

Enhanced angiogenesis in bFGF-containing scaffold promoted viability of enclosed hepatocytes and maintained hepatospecific glycogen storage capacity

T Takei, H Ijima, S Sakai, T Ono, K Kawakami

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN　Vol. 38　No. 11　p. 913-917　2005/11　Research paper (scientific journal)

Subsieve-size agarose capsules enclosing ifosfamide-activating cells: a strategy toward chemotherapeutic targeting to tumors

S Sakai, K Kawabata, S Tanaka, N Harimoto, Hashimoto, I, CJ Mu, B Salmons, H Ijima, K Kawakami

MOLECULAR CANCER THERAPEUTICS　Vol. 4　No. 11　p. 1786-1790　2005/11　Research paper (scientific journal)

Development of mammalian cell-enclosing subsieve-size agarose capsules (< 100 mu m) for cell therapy

S Sakai, K Kawabata, T Ono, H Ijima, K Kawakami

BIOMATERIALS　Vol. 26　No. 23　p. 4786-4792　2005/08　Research paper (scientific journal)

Development of mammalian cell-enclosing subsieve-size agarose capsules (< 100 mu m) for cell therapy

S Sakai, K Kawabata, T Ono, H Ijima, K Kawakami

BIOMATERIALS　Vol. 26　No. 23　p. 4786-4792　2005/08　Research paper (scientific journal)

Transition of mechanical property of porous alginate scaffold with cells during culture period.

Sakai S, Masuhara H, Yamada Y, Ono T, Ijima H, Kawakami K

Journal of bioscience and bioengineering　Vol. 100　No. 1　p. 127-129　2005/07

Transition of mechanical property of porous alginate scaffold with cells during culture period

S Sakai, H Masuhara, Y Yamada, T Ono, H Ijima, K Kawakami

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　Vol. 100　No. 1　p. 127-129　2005/07　Research paper (scientific journal)

Higher viscous solution induces smaller droplets for cell-enclosing capsules in a co-flowing stream

Sakai, Shinji, Kawabata, Kenji, Ono, Tsutomu, Ijima, Hiroyuki, Kawakami, Koei

Biotechnology Progress　Vol. 21　No. 3　p. 994-997　2005/05　Research paper (scientific journal)

Higher viscous solution induces smaller droplets for cell-enclosing capsules in a co-flowing stream

S Sakai, K Kawabata, T Ono, H Ijima, K Kawakami

BIOTECHNOLOGY PROGRESS　Vol. 21　No. 3　p. 994-997　2005/05　Research paper (scientific journal)

動物細胞包括サブシーブカプセルの性能評価

境 慎司, 川端 賢二, 小野 努, 井嶋 博之, 川上 幸衛

化学工学会 研究発表講演要旨集　Vol. 2005　p. 318-318　2005

アミノ糖添加によるタンパク質含有リポソームの特性改変とその評価

村上 直, 境 慎司, 小野 努, 井嶋 博之, 川上 幸衛

化学工学会 研究発表講演要旨集　Vol. 2005　p. 204-204　2005

生体外におけるゲル支持体を用いた血管の構築

武井 孝行, 境 慎司, 小野 努, 井嶋 博之, 川上 幸衛

化学工学会 研究発表講演要旨集　Vol. 2005　p. 212-212　2005

Development and characterization of a silica monolith immobilized enzyme micro-bioreactor

K Kawakami, Y Sera, S Sakai, T Ono, H Ijima

INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH　Vol. 44　No. 1　p. 236-240　2005/01　Research paper (scientific journal)

Behavior of enclosed sol- and gel-alginates in vivo

S Sakai, T Ono, H Ijima, K Kawakami

BIOCHEMICAL ENGINEERING JOURNAL　Vol. 22　No. 1　p. 19-24　2004/12　Research paper (scientific journal)

Preparation of mammalian cell-enclosing subsieve-sized capsules (< 100 mu m) in a coflowing stream

S Sakai, K Kawabata, T Ono, H Ijima, K Kawakami

BIOTECHNOLOGY AND BIOENGINEERING　Vol. 86　No. 2　p. 168-173　2004/04　Research paper (scientific journal)

Preparation of mammalian cell-enclosing subsieve-sized capsules (< 100 mu m) in a coflowing stream

S Sakai, K Kawabata, T Ono, H Ijima, K Kawakami

BIOTECHNOLOGY AND BIOENGINEERING　Vol. 86　No. 2　p. 168-173　2004/04　Research paper (scientific journal)

MIN6 cells-enclosing aminopropyl-silicate membrane templated by alginate gels differences in guluronic acid content

S Sakai, T Ono, H Ijima, K Kawakami

INTERNATIONAL JOURNAL OF PHARMACEUTICS　Vol. 270　No. 1-2　p. 65-73　2004/02　Research paper (scientific journal)

MIN6 cell-enclosing aminopropyl-silicate membrane templated by alginate gels differences in guluronic acid content

Shinji Sakai, Tsutomu Ono, Hiroyuki Ijima, Koei Kawakami

International Journal of Pharmaceutics　2004/02　Research paper (scientific journal)

Development of DNA-Containing Nano Scale Vesicle by Reverse Micelles

Morita Mitsuhiro, Ono Tsutomu, Sakai Shinji, Ijima Hiroyuki, Kawakami Koei

Asian Pacific Confederation of Chemical Engineering congress program and abstracts　Vol. 2004　p. 478-478　2004

Development of bFGF Sustained-Release Bioabsorbable Sponge and Enhancement of Angiogenesis in it

Takei Takayuki, Ijima Hiroyuki, Sakai Shinji, Ono Tsutomu, Kawakami Koei

Asian Pacific Confederation of Chemical Engineering congress program and abstracts　Vol. 2004　p. 480-480　2004

Proliferation and insulin secretion function of mouse insulinoma cells encapsulated in alginate/sol-gel synthesized aminopropyl-silicate/alginate microcapsule

S Sakai, T Ono, H Ijima, K Kawakami

JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY　Vol. 28　No. 2　p. 267-272　2003/09　Research paper (scientific journal)

Modification of porous aminopropyl-silicate microcapsule membrane by electrically-bonded external anionic polymers

S Sakai, T Ono, H Ijima, K Kawakami

JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION　Vol. 14　No. 7　p. 643-652　2003　Research paper (scientific journal)

Alginate/aminopropyl-silicate/alginate membrane immunoisolatability and insulin secretion of encapsulated islets

S Sakai, T Ono, Ijima, I, K Kawakami

BIOTECHNOLOGY PROGRESS　Vol. 18　No. 2　p. 401-403　2002/03　Research paper (scientific journal)

In vitro and in vivo evaluation of alginate/sol-gel synthesized aminopropyl-silicate/alginate membrane for bioartificial pancreas

Shinji Sakai, Tsutomu Ono, Hiroyuki Ijima, Koei Kawakami

Biomaterials　Vol. 23　No. 21　p. 4177-4183　2002　Research paper (scientific journal)

Permeability of sol-gel synthesized aminopropyl-silicate-titanate hybrid membrane toward microcapsule-shaped bioartificial pancreas

Shinji Sakai, Tsutomu Ono, Hiroyuki Ijima, Koei Kawakami

Journal of Artificial Organs　Vol. 5　p. 54-59　2002/01　Research paper (scientific journal)

Aminopropyl-silicate membrane for microcapsule-shaped bioartificial organs

Shinji Sakai, Tsutomu Ono, Hiroyuki Ijima, Koei Kawakami

Control of molecular permeability, Journal of Membrane Science　2002/01　Research paper (scientific journal)

Permeability of alginate/sol-gel synthesized aminopropyl-silicate/alginate membrane templated by calcium-alginate gel

Shinji Sakai, Tsutomu Ono, Hiroyuki Ijima, Koei Kawakami

Journal of Membrane Science　2002/01　Research paper (scientific journal)

Control of transport characteristic of membrane by multi-layering of polyelectrolyte complex toward microcapsule-shaped bioartificial pancreas

Sakai, S., Ono, T., Ijima, H., Kawakami, K.

Kagaku Kogaku Ronbunshu　Vol. 27　No. 2　p. 168-168　2001/12/01　Research paper (scientific journal)

Control of Transport Characteristic of Membrane by Multi-layering of Polyelectrolyte Complex Toward Microcapsule-shaped Bioartificial Pancreas

Shinji Sakai, Tsutomu Ono, Hiroyuki Ijima, Kofi Kawakami

Kagaku Kogaku Ronbunshu　Vol. 27　No. 2　p. 165-168　2001　Research paper (scientific journal)

Synthesis and transport characterization of alginate/aminopropyl-silicate/alginate microcapsule: Application to bioartificial pancreas

Shinji Sakai, Tsutomu Ono, Hiroyuki Ijima, Koei Kawakami

Biomaterials　Vol. 22　No. 21　p. 2827-2834　2001　Research paper (scientific journal)

Newly developed aminopropyl-silicate immunoisolation membrane for a microcapsule-shaped bioartificial pancreas

S Sakai, T Ono, H Ijima, K Kawakami

BIOARTIFICIAL ORGANS III: TISSUE SOURCING, IMMUNOISOLATION, AND CLINICAL TRIALS　Vol. 944　p. 277-283　2001　Research paper (scientific journal)

Control of molecular weight cut-off immunoisolation by multilayering glycol chitosan-alginate polyion complex on alginate-based microcapsules

Sakai, S., Ono, T., Ijima, H., Kawakami, K.

Journal of Microencapsulation　Vol. 17　No. 6　2000/01　Research paper (scientific journal)