顔写真

PHOTO

Motone Keisuke
元根 啓佑
Motone Keisuke
元根 啓佑
Graduate School of Engineering Center for Future Innovation, Assistant Professor
motone.keisuke.rbc osaka-u.ac.jp

keyword Single-molecule protein sequencing,Bioanalysis engineering,nanopore proteomics,measurement technology development,sequence data analysis,amino acid sequencing,High-throughput analysis,Bioinformatics,Drug discovery,PPI,Bioengineering,Antibody,Biomolecule interactions,Next-generation protein sequencing,Post-translational modification,Highly multiplexed analysis,Single-molecule analysis,Synthetic biology,Biotechnology development,Single-molecule proteomics,Nanopores

Research History 6

  1. 2024/11 - Present
    Osaka University Graduate School of Engineering Principal Investigator

  2. 2024/06 - Present
    Osaka University Center for Future Innovation (CFi), Graduate School of Engineering Assistant Professor

  3. 2021/05 - 2025/03
    Osaka Metropolitan University Department of Applied Biological Chemistry Visiting Scholar

  4. 2020/04 - 2024/06
    University of Washington Paul G. Allen School of Computer Science and Engineering Postdoctoral Scholar

  5. 2020/04 - 2022/04
    University of Washington Paul G. Allen School of Computer Science and Engineering JSPS 海外特別研究員

  6. 2017/04 - 2020/03
    Kyoto University

Education 2

  1. Kyoto University Graduate School of Agriculture Division of Applied Life Sciences

    2015/04 - 2020/03

  2. Kyoto University Faculty of Agriculture Applied Life Sciences

    2010/04 - 2015/03

Committee Memberships 2

  1. 日本生物工学会 若手会 2025年度生物工学若手会夏のセミナー実行委員 Academic society

    2024 - 2025

  2. The 25th Annual Meeting of the Protein Science Society of Japan Commitee Member Academic society

    2025 -

Professional Memberships 3

  1. Japan Society for Bioscience, Biotechnology, and Agrochemistry

  2. 日本生物工学会

  3. 日本蛋白質科学会

Research Areas 5

  1. Life sciences / Pharmaceuticals - analytical and physicochemistry /

  2. Life sciences / Applied biochemistry /

  3. Manufacturing technology (mechanical, electrical/electronic, chemical engineering) / Applied biofunctional and bioprocess engineering /

  4. Nanotechnology/Materials / Nanobioscience / nanopores

  5. Life sciences / Applied microbiology /

Awards 4

  1. Young Scientist Excellence Award

    Keisuke Motone Protein Science Society of Japan 2024/06

  2. Travel Grants for Early Career Researchers in Overseas

    Keisuke Motone Travel Grant 2023/12

  3. 第一種奨学金 全額返済免除

    元根 啓佑 JASSO 2020/03

  4. 優秀ポスター賞

    元根 啓佑 生物工学若手研究者の集い(若手会)夏のセミナー2018 2018/07

Papers 23

  1. Multi-pass, single-molecule nanopore reading of long protein strands

    Keisuke Motone, Daphne Kontogiorgos-Heintz, Jasmine Wee, Kyoko Kurihara, Sangbeom Yang, Gwendolin Roote, Oren E. Fox, Yishu Fang, Melissa Queen, Mattias Tolhurst, Nicolas Cardozo, Miten Jain, Jeff Nivala

    Nature Vol. 633 No. 8030 p. 662-669 2024/09/11 Research paper (scientific journal)

    Publisher: Springer Science and Business Media LLC
  2. Not if but when nanopore protein sequencing meets single-cell proteomics.

    Keisuke Motone, Jeff Nivala

    Nature Methods Vol. 20 No. 3 p. 336-338 2023/03/10 Research paper (scientific journal)

  3. Herding cats: Label-based approaches in protein translocation through nanopore sensors for single-molecule protein sequence analysis.

    Keisuke Motone, Nicolas Cardozo, Jeff Nivala

    iScience Vol. 24 No. 9 2021/09/24 Research paper (scientific journal)

  4. ナノポアを用いた1分子タンパク質計測の課題と展望

    元根 啓佑

    PSSJ Archives Vol. 18 2025/02/17

  5. Complete genome sequence of Flavobacteriaceae sp. strain GF1, isolated from the phycosphere of a coral endosymbiotic dinoflagellate

    Toshiyuki Takagi, Kako Aoyama, Keisuke Motone

    Microbiology Resource Announcements 2024/12/31 Research paper (scientific journal)

    Publisher: American Society for Microbiology
  6. 次世代タンパク質シーケンサーはプロテオミクスを変革するか

    元根啓佑

    生物工学会誌 Vol. 102 No. 10 2024/10/25

  7. Interpreting and modeling nanopore ionic current signals during unfoldase-mediated translocation of single protein molecules

    Daphne Kontogiorgos-Heintz, Keisuke Motone, Jeff Nivala

    Biophysical Journal Vol. 123 No. 3 2024/02/08 Research paper (international conference proceedings)

    Publisher: Elsevier BV
  8. Characterization of carotenoid-producing Muricauda sp. strains isolated from the reef-building coral Galaxea fascicularis and subtropical seawater

    Funa Endo, Keisuke Motone, Kenji Kai, Tomoya Kitakaze, Yumi Nishikawa, Yuna Nishimura, Ruriko Kitamura, Toshiyuki Takagi, Michihiro Ito, Natsuko Miura, Michihiko Kataoka

    bioRxiv 2023/12/13

    Publisher: Cold Spring Harbor Laboratory
  9. Multi-pass, single-molecule nanopore reading of long protein strands with single-amino acid sensitivity.

    Keisuke Motone, Daphne Kontogiorgos-Heintz, Jasmine Wee, Kyoko Kurihara, Sangbeom Yang, Gwendolin Roote, Yishu Fang, Nicolas Cardozo, Jeff Nivala

    bioRxiv 2023/10/20

  10. Mutualistic Interactions between Dinoflagellates and Pigmented Bacteria Mitigate Environmental Stress.

    Toshiyuki Takagi, Kako Aoyama, Keisuke Motone, Shunsuke Aburaya, Hideyuki Yamashiro, Natsuko Miura, Koji Inoue

    Microbiology spectrum Vol. 11 No. 1 2023/02/14 Research paper (scientific journal)

  11. Evaluation of a library of loxP variants with a wide range of recombination efficiencies by Cre.

    Yuji Yamauchi, Hidenori Matsukura, Keisuke Motone, Mitsuyoshi Ueda, Wataru Aoki

    PloS one Vol. 17 No. 10 2022 Research paper (scientific journal)

  12. Design of novel primer sets for easy detection of Ruegeria species from seawater.

    Ruriko Kitamura, Natsuko Miura, Keiko Okada, Keisuke Motone, Toshiyuki Takagi, Mitsuyoshi Ueda, Michihiko Kataoka

    Bioscience, biotechnology, and biochemistry Vol. 84 No. 4 p. 854-864 2020/04 Research paper (scientific journal)

  13. A Zeaxanthin-Producing Bacterium Isolated from the Algal Phycosphere Protects Coral Endosymbionts from Environmental Stress.

    Keisuke Motone, Toshiyuki Takagi, Shunsuke Aburaya, Natsuko Miura, Wataru Aoki, Mitsuyoshi Ueda

    mBio Vol. 11 No. 1 2020/01/21 Research paper (scientific journal)

  14. Ruegeria sp. Strains Isolated from the Reef-Building Coral Galaxea fascicularis Inhibit Growth of the Temperature-Dependent Pathogen Vibrio coralliilyticus.

    Natsuko Miura, Keisuke Motone, Toshiyuki Takagi, Shunsuke Aburaya, Sho Watanabe, Wataru Aoki, Mitsuyoshi Ueda

    Marine Biotechnology Vol. 21 No. 1 p. 1-8 2019/02 Research paper (scientific journal)

  15. Protection of Coral Larvae from Thermally Induced Oxidative Stress by Redox Nanoparticles.

    Keisuke Motone, Toshiyuki Takagi, Shunsuke Aburaya, Wataru Aoki, Natsuko Miura, Hiroyoshi Minakuchi, Haruko Takeyama, Yukio Nagasaki, Chuya Shinzato, Mitsuyoshi Ueda

    Marine Biotechnology Vol. 20 No. 4 p. 542-548 2018/08 Research paper (scientific journal)

  16. Direct bioethanol production from brown macroalgae by co-culture of two engineered Saccharomyces cerevisiae strains.

    Yusuke Sasaki, Toshiyuki Takagi, Keisuke Motone, Toshiyuki Shibata, Kouichi Kuroda, Mitsuyoshi Ueda

    Bioscience, biotechnology, and biochemistry Vol. 82 No. 8 p. 1459-1462 2018/08 Research paper (scientific journal)

  17. Redox nanoparticle prevents coral larvae from heat stress mortality as a potential use for coral reef protection

    Motone, Keisuke, Takagi, Toshiyuki, Aburaya, Shunsuke, Aoki, Wataru, Miura, Natsuko, Minakuchi, Hiroyoshi, Takeyama, Haruko, Nagasaki, Yukio

    Marine Biotechnology 2018/04 Research paper (scientific journal)

  18. High-throughput evaluation of T7 promoter variants using biased randomization and DNA barcoding.

    Ryo Komura, Wataru Aoki, Keisuke Motone, Atsushi Satomura, Mitsuyoshi Ueda

    PloS one Vol. 13 No. 5 2018 Research paper (scientific journal)

  19. Construction of bioengineered yeast platform for direct bioethanol production from alginate and mannitol.

    Toshiyuki Takagi, Yusuke Sasaki, Keisuke Motone, Toshiyuki Shibata, Reiji Tanaka, Hideo Miyake, Tetsushi Mori, Kouichi Kuroda, Mitsuyoshi Ueda

    Applied microbiology and biotechnology Vol. 101 No. 17 p. 6627-6636 2017/09 Research paper (scientific journal)

  20. Enhanced direct ethanol production by cofactor optimization of cell surface-displayed xylose isomerase in yeast.

    Yusuke Sasaki, Toshiyuki Takagi, Keisuke Motone, Kouichi Kuroda, Mitsuyoshi Ueda

    Biotechnology progress Vol. 33 No. 4 p. 1068-1076 2017/07 Research paper (scientific journal)

  21. Direct ethanol fermentation of the algal storage polysaccharide laminarin with an optimized combination of engineered yeasts.

    Keisuke Motone, Toshiyuki Takagi, Yusuke Sasaki, Kouichi Kuroda, Mitsuyoshi Ueda

    Journal of biotechnology Vol. 231 p. 129-135 2016/08/10 Research paper (scientific journal)

  22. Ethanol production from hemicellulose using xylose isomerase-displaying yeast

    Sasaki Yusuke, Takagi Toshiyuki, Motone Keisuke, Kuroda Kouichi, Ueda Mitsuyoshi

    NEW BIOTECHNOLOGY Vol. 33 2016/07/25

  23. Platform of direct ethanol production from macroalgae by engineered Saccharomyces cerevisiae

    Motone Keisuke, Takagi Toshiyuki, Sasaki Yusuke, Kuroda Kouichi, Ueda Mitsuyoshi

    NEW BIOTECHNOLOGY Vol. 33 2016/07/25

Misc. 15

  1. ナノポア計測が切り拓く1分子タンパク質シーケンシングの未来

    元根啓佑, KONTOGIORGOS-HEINTZ Daphne, NIVALA Jeff

    化学とマイクロ・ナノシステム学会研究会講演要旨集(CD-ROM) Vol. 49th 2024

  2. Establishing axenic Symbiodiniaceae strains for microbiome manipulation

    高木俊幸, 青山華子, 青山華子, 元根啓佑, 元根啓佑, 油屋駿介, 遠藤芙奈, 三浦夏子, 井上広滋, 井上広滋

    日本サンゴ礁学会大会講演要旨集(Web) Vol. 26th 2023

  3. Mutualistic interactions between dinoflagellates and pigmented bacteria mitigate environmental stress

    高木俊幸, 青山華子, 青山華子, 元根啓佑, 元根啓佑, 油屋駿介, 山城秀之, 三浦夏子, 井上広滋

    日本サンゴ礁学会大会講演要旨集(Web) Vol. 25th 2022

  4. アザミサンゴGalaxea fascicularisから単離したRuegeria属細菌の解析

    三浦夏子, 岡田圭以子, 元根啓佑, 元根啓佑, 北村瑠璃子, 高木俊幸, 植田充美, 片岡道彦

    日本農芸化学会大会講演要旨集(Web) Vol. 2019 2019

  5. サンゴに共在するRuegeria属細菌の分布調査を目的とした新規プライマーの設計

    北村瑠璃子, 三浦夏子, 岡田圭以子, 元根啓佑, 元根啓佑, 高木俊幸, 植田充美, 片岡道彦

    日本農芸化学会大会講演要旨集(Web) Vol. 2019 2019

  6. Ruegeria属細菌の分布調査を目的とした環境DNA検出法の検討

    北村瑠璃子, 三浦夏子, 岡田圭以子, 元根啓佑, 元根啓佑, 高木俊幸, 植田充美, 片岡道彦

    日本農芸化学会西日本支部大会およびシンポジウム講演要旨集 Vol. 2019 2019

  7. サンゴから単離した共在微生物による病原菌の生育抑制機能評価

    三浦夏子, 元根啓佑, 元根啓佑, 高木俊幸, 高木俊幸, 植田充美

    日本農芸化学会大会講演要旨集(Web) Vol. 2018 2018

  8. サンゴ共生褐虫藻の単離・培養条件の検討

    元根啓佑, 元根啓佑, 三浦夏子, 高木俊幸, 高木俊幸, 青木航, 植田充美

    日本農芸化学会大会講演要旨集(Web) Vol. 2018 2018

  9. ボトムアップジェネティクスによる生物学的システム再構成のハイスループット化

    青木航, 古村崚, 元根啓佑, 元根啓佑, 植田充美

    日本分子生物学会年会プログラム・要旨集(Web) Vol. 41st 2018

  10. バイアスドランダム化とDNAバーコード付加によるプロモーター変異体のハイスループット解析

    青木航, 青木航, 古村崚, 元根啓佑, 元根啓佑, 植田充美

    日本生物工学会大会講演要旨集 Vol. 70th 2018

  11. 大型藻類主要多糖であるアルギン酸を直接資化する酵母の分子育種

    黒田浩一, 高木俊幸, 佐々木勇輔, 元根啓佑, 植田充美

    日本農芸化学会大会講演要旨集(Web) Vol. 2018 2018

  12. 細胞内レドックスバランスを維持できるキシロース資化酵母の性能向上

    佐々木勇輔, 佐々木勇輔, 高木俊幸, 高木俊幸, 元根啓佑, 元根啓佑, 黒田浩一, 黒田浩一, 植田充美, 植田充美

    日本農芸化学会大会講演要旨集(Web) Vol. 2017 2017

  13. 地球温暖化により消滅の危機にあるサンゴ礁の修復(その1)-Redox nanoparticleによる高温ストレス下でのサンゴ幼生の生存率の向上-

    元根啓佑, 高木俊幸, 高木俊幸, 油屋駿介, 油屋駿介, 新里宙也, 長崎幸夫, 竹山春子, 青木航, 植田充美

    日本農芸化学会大会講演要旨集(Web) Vol. 2017 2017

  14. サンゴ共生体の保護を目指した取り組み-抗活性酸素剤による高温ストレス下でのサンゴ幼生の生存率の向上-

    元根啓佑, 元根啓佑, 高木俊幸, 高木俊幸, 油屋駿介, 油屋駿介, 新里宙也, 長崎幸夫, 竹山春子, 青木航, 植田充美

    日本微生物生態学会大会(Web) Vol. 2017 2017

  15. 大型藻類有効利用に向けたラミナリン発酵酵母の分子育種

    元根啓佑, 元根啓佑, 高木俊幸, 高木俊幸, 佐々木勇輔, 佐々木勇輔, 黒田浩一, 黒田浩一, 植田充美, 植田充美

    日本生物工学会大会講演要旨集 Vol. 67th 2015

Publications 1

  1. 第24回日本蛋白質科学会年会(札幌)若手奨励賞受賞者コラム

    元根 啓佑

    日本蛋白質科学会ニュースレター Vol. 24, No. 8 (2024) 2024/07/25

Presentations 17

  1. Can We Read Proteins? Nanopore Technology Paving the Way for Next-Generation Proteomics

    Keisuke Motone

    The 98th Annual Meeting of the Japanese Biochemical Society 2025/11/04

  2. 未踏への挑戦:ナノポアでタンパク質は読めるか?

    元根 啓佑

    「細胞を創る」研究会 18.0 2025/09/17

  3. Nanopore proteomics: multifaceted analysis of protein sequence, structure, and post-translational modifications

    Keisuke Motone

    2025/09/11

  4. Unfoldase-driven nanopore translocation of single protein strands for sequence, structural, and post-translational modification analysis

    Keisuke Motone

    The 25th Annual Meeting of the Protein Science Society of Japan 2025/06/18

  5. 逆張りのススメ

    元根 啓佑

    第5回 蛋白質科学会若手の会 研究交流会 2025/06/17

  6. Revolutionizing proteomics with next-generation protein sequencing

    Keisuke Motone

    The 145th Annual Meeting of the Pharmaceutical Society of Japan (Fukuoka) 2025/03/27

  7. An unfoldase-coupled nanopore translocation system for detecting point mutations and post-translational modifications along protein strands

    Keisuke Motone

    The Nanopore Meeting 2025 in Tokyo 2025/03/14

  8. Revolutionizing proteomics with nanopore technology

    Keisuke Motone

    The 26th Academic Exchange Seminar between Shanghai Jiao Tong University and Osaka University 2024/11/08

  9. Development of nanopore techniques for next-generation protein sequencing

    Keisuke Motone

    2024/10/31

  10. ナノポア計測でタンパク質分析を「次世代化」する

    元根 啓佑

    第1514回生物科学セミナー 2024/10/30

  11. Nanopore proteomics

    Keisuke Motone

    2024/10/01

  12. ナノポアプロテオミクス — アミノ酸配列と翻訳後修飾の1分子読み取り —

    元根 啓佑

    生物工学若手研究者の集い(若手会)夏のセミナー2024 2024/07/13

  13. Multiplexed nanopore reading of single-molecule protein strands and post-translational modifications

    Keisuke Motone

    The 24th Annual Meeting of the Protein Science Society of Japan 2024/06/12

  14. ナノポアプロテオミクス — アミノ酸配列と翻訳後修飾の1分子解読 —

    Keisuke Motone

    第4回 蛋白質科学会若手の会 研究交流会 2024/06/10

  15. Toward nanopore-based single-molecule protein sequencing

    Keisuke Motone

    CHEMINAS 49 2024/06/01

  16. Progress towards long-read single-molecule protein sequencing on an array of unfoldase-coupled nanopores

    Keisuke Motone, Jeff Nivala

    2023 Advances in Genomic Technology Development Annual Meeting 2023/06/06

  17. Routes to processive nanopore protein translocation with molecular motors

    Keisuke Motone, Jeff Nivala

    Nanopore Sequencing: From Genomes to Proteomes 2022/05/10

Industrial Property Rights 1

  1. METHOD AND KIT FOR REREADING SINGLE PROTEIN MOLECULES USING A NANOPORE AND AN UNFOLDASE

    JEFFREY MATTHEW NIVALA, KEISUKE MOTONE

    WO/2024/249122

    出願日:2024/05/17

Social Activities 1

  • 四條畷高校 研究室見学

    2024/08 -

Media Coverage 6

  1. Multi-pass nanopore for single-molecule protein sequencing

    Nature Methods

    2024/11/07

  2. A strategy to load, rethread and read protein sequences through a nanopore

    Nature Biotechnology

    2024/10/14

  3. Breakthrough towards protein sequencing using Oxford Nanopore holds promise for disease research and drug development

    Oxford Nanopore Technologies

    2024/09/12

  4. 生物工学:長鎖タンパク質のマルチパス、単一分子ナノポア読み取り

    Nature Japan

    2024/09/11

  5. Thread, read, rewind, repeat: towards using nanopores for protein sequencing

    Nature

    2024/09/11

  6. Next Generation Protein Sequencing: Achieving Single-Molecule Sensitivity and Full-Length Proteoform Identification

    preLights

    2023/12/04

Academic Activities 3

  1. Innovative biotechnology that opens new horizons in bioengineering

    2025/09/11 -

  2. Reviewer (source-work-id: a963a99ab7df5c17ed9620500b69c6af)

    Nature Nanotechnology

    2025 -

  3. Reviewer (SOURCE-WORK-ID: 18dcac338fec397196d5172d61001659)

    Nature Nanotechnology

    2024 -