Production and N-glycan engineering of Varlilumab in Nicotiana benthamiana

Kim Dua Nguyen, Hiroyuki Kajiura, Ryo Kamiya, Takahiro Yoshida, Ryo Misaki, Kazuhito Fujiyama

Frontiers in Plant Science　Vol. 14　2023/08/08　Research paper (scientific journal)

High accumulation of the Man5GlcNAc2 structure by combining N-acetylglucosaminyltransferase I gene suppression and mannosidase I gene overexpression in Nicotiana tabacum SR1

Keigo Sato, Hitomi Yumioka, Junko Isoyama, Koji Dohi, Akihiro Yamanaka, Takao Ohashi, Ryo Misaki, Kazuhito Fujiyama

Journal of Bioscience and Bioengineering　Vol. 136　No. 2　p. 136-141　2023/08　Research paper (scientific journal)

バイオ医薬品製造に用いる細胞基材の樹立に関する留意事項

康博 岸岡, 稔 多田, 亮佑 栗林, 貴之 堀内, 知朗 高倉, 範子 山野-足立, 亮 三﨑, 純 石井, 康勝 太田, 智資 大屋, 和彦 片山, 彰人 川原, 伸吾 小山, 日登志 阪下, 靖 瀧村, 拓也 森本, 歩希 中野, 健二 早水, 昌司 日向, 聖 村上, 和久 内田, 健史 大政, 明子 石井

日本PDA学術誌 GMPとバリデーション　2023/06　Research paper (scientific journal)

The specific core fucose-binding lectin Pholiota squarrosa lectin (PhoSL) inhibits hepatitis B virus infection in vitro.

Tsunenori Ouchida, Haruka Maeda, Yuka Akamatsu, Megumi Maeda, Shinji Takamatsu, Jumpei Kondo, Ryo Misaki, Yoshihiro Kamada, Masahiro Ueda, Keiji Ueda, Eiji Miyoshi

Scientific reports　Vol. 13　No. 1　p. 6175-6175　2023/04/15　Research paper (scientific journal)

Effects of various disaccharide adaptations on recombinant IgA1 production in CHO-K1 suspension cells

John Benson D. Choa, Tadahiro Sasaki, Hiroyuki Kajiura, Kazuyoshi Ikuta, Kazuhito Fujiyama, Ryo Misaki

Cytotechnology　Vol. 75　No. 3　p. 219-229　2023/03/16　Research paper (scientific journal)

High-level transient production of a protease-resistant mutant form of human basic fibroblast growth factor in Nicotiana benthamiana leaves.

Edjohn Aaron Macauyag, Hiroyuki Kajiura, Takao Ohashi, Ryo Misaki, Kazuhito Fujiyama

Plant biotechnology (Tokyo, Japan)　Vol. 39　No. 3　p. 291-301　2022/09/25　Research paper (scientific journal)

Production of recombinant β-glucocerebrosidase in wild-type and glycoengineered transgenic Nicotiana benthamiana root cultures with different N-glycan profiles

Naphatsamon Uthailak, Hiroyuki Kajiura, Ryo Misaki, Kazuhito Fujiyama

Journal of Bioscience and Bioengineering　Vol. 133　No. 5　p. 481-488　2022/05　Research paper (scientific journal)

Establishment of serum-free adapted Chinese hamster ovary cells with double knockout of GDP-mannose-4,6-dehydratase and GDP-fucose transporter.

Ryo Misaki, Masashi Iwasaki, Hiroki Takechi, Noriko Yamano-Adachi, Takao Ohashi, Hiroyuki Kajiura, Kazuhito Fujiyama

Cytotechnology　Vol. 74　No. 1　p. 163-179　2022/02　Research paper (scientific journal)

Biochemical characterization of Arabidopsis clade F polygalacturonase shows a substrate preference toward oligogalacturonic acids

Takao Ohashi, Nabilah Sari, Ryo Misaki, Kazuhito Fujiyama

Journal of Bioscience and Bioengineering　Vol. 133　No. 1　p. 1-7　2022/01　Research paper (scientific journal)

Analysis of N-glycan profile of Arabidopsis alg3 cell culture.

Ratna Sariyatun, Hiroyuki Kajiura, Juthamard Limkul, Ryo Misaki, Kazuhito Fujiyama

Plant biotechnology (Tokyo, Japan)　Vol. 38　No. 4　p. 463-467　2021/12/25　Research paper (scientific journal)

Bombyx mori β1,4-N-acetylgalactosaminyltransferase possesses relaxed donor substrate specificity in N-glycan synthesis

Hiroyuki Kajiura, Ryousuke Miyauchi, Akemi Kakudo, Takao Ohashi, Ryo Misaki, Kazuhito Fujiyama

Scientific Reports　Vol. 11　No. 1　2021/12　Research paper (scientific journal)

Improved assay system for acidic peptide: N-glycanase (aPNGase) activity in plant extracts

Chiharu Yamamoto, Mikako Ogura, Ryota Uemura, Maeda Megumi, Hiroyuki Kajiura, Ryo Misaki, Kazuhito Fujiyama, Yoshinobu Kimura

Analytical Biochemistry　p. 114367-114367　2021/09　Research paper (scientific journal)

Production of Human Acid-Alpha Glucosidase With a Paucimannose Structure by Glycoengineered Arabidopsis Cell Culture

Ratna Sariyatun, Florence, Hiroyuki Kajiura, Takao Ohashi, Ryo Misaki, Kazuhito Fujiyama

Frontiers in Plant Science　Vol. 12　2021/07/14　Research paper (scientific journal)

Transient Production of Human β-Glucocerebrosidase With Mannosidic-Type N-Glycan Structure in Glycoengineered Nicotiana benthamiana Plants

Naphatsamon Uthailak, Hiroyuki Kajiura, Ryo Misaki, Kazuhito Fujiyama

Frontiers in Plant Science　Vol. 12　2021/06/07　Research paper (scientific journal)

Direct evidence of cytosolic PNGase activity in Arabidopsis thaliana: in vitro assay system for plant cPNGase activity

Sahoko Shirai, Ryota Uemura, Megumi Maeda, Hiroyuki Kajiura, Ryo Misaki, Kazuhito Fujiyama, Yoshinobu Kimura

Bioscience, Biotechnology, and Biochemistry　Vol. 85　No. 6　p. 1460-1463　2021/05/25　Research paper (scientific journal)

Rab11-mediated post-Golgi transport of the sialyltransferase ST3GAL4 suggests a new mechanism for regulating glycosylation.

Masato Kitano, Yasuhiko Kizuka, Tomoaki Sobajima, Miyako Nakano, Kazuki Nakajima, Ryo Misaki, Saki Itoyama, Yoichiro Harada, Akihiro Harada, Eiji Miyoshi, Naoyuki Taniguchi

The Journal of biological chemistry　Vol. 296　p. 100354-100354　2021/01/29　Research paper (scientific journal)

Structure and Biological Functions of Plant Glycans and Polysaccharides

Ryo Misaki, Kazuhito Fujiyama, Hiroyuki Kajiura

Comprehensive Glycoscience　p. 93-109　2021　Part of collection (book)

Functional characterization and overexpression of Δ12-desaturase in the oleaginous yeast Rhodotorula toruloides for production of linoleic acid-rich lipids

Chih Chan Wu, Takao Ohashi, Hiroyuki Kajiura, Yu Sato, Ryo Misaki, Kohsuke Honda, Savitree Limtong, Kazuhito Fujiyama

Journal of Bioscience and Bioengineering　2021　Research paper (scientific journal)

Transglycosylation toward naringenin-7-O-glucoside using an N180H mutant of Coprinopsis cinerea endo-β-N-acetylglucosaminidase

Takao Ohashi, Yu Fujisawa, Marc R. Hayes, Ryo Misaki, Jörg Pietruszka, Kazuhito Fujiyama

Biochemical and Biophysical Research Communications　Vol. 530　No. 1　p. 155-159　2020/09/10　Research paper (scientific journal)

Characterization of Bombyx mori N-acetylglucosaminyltransferase II splicing variants

Hiroyuki Kajiura, Yugo Nakamura, Mari Nishimura, Takao Ohashi, Ryo Misaki, Kazuhito Fujiyama

Biochemical and Biophysical Research Communications　Vol. 529　No. 2　p. 404-410　2020/08　Research paper (scientific journal)

Enhancement of sialylation in rIgG in glyco-engineered Chinese hamster ovary cells

Thi Sam Nguyen, Ryo Misaki, Takao Ohashi, Kazuhito Fujiyama

Cytotechnology　Vol. 72　No. 3　p. 343-355　2020/06/01　Research paper (scientific journal)

Ethanol and H2O2 stresses enhance lipid production in an oleaginous Rhodotorula toruloides thermotolerant mutant L1-1.

Chih-Chan Wu, Takao Ohashi, Ryo Misaki, Savitree Limtong, Kazuhito Fujiyama

FEMS yeast research　Vol. 20　No. 4　2020/06/01　Research paper (scientific journal)

Fully Human Monoclonal Antibodies Effectively Neutralizing Botulinum Neurotoxin Serotype B.

Takuhiro Matsumura, Sho Amatsu, Ryo Misaki, Masahiro Yutani, Anariwa Du, Tomoko Kohda, Kazuhito Fujiyama, Kazuyoshi Ikuta, Yukako Fujinaga

Toxins　Vol. 12　No. 5　2020/05/07　Research paper (scientific journal)

Arabidopsis thaliana α1,2-L-fucosyltransferase catalyzes the transfer of L-galactose to xyloglucan oligosaccharide

Ohashi H, Ohashi T, Misaki R, Fujiyama K

FEBS Lett　Vol. 593　No. 2　p. 187-194　2019/01　Research paper (scientific journal)

Transcriptome sequencing and identification of cytochrome p450 monooxygenases involved in the biosynthesis of maslinic acid and corosolic acid in Avicennia marina

Mitsuki Nakamura, Tran My Linh, Le Quynh Lien, Hayato Suzuki, Nguyen Chi Mai, Vu Huong Giang, Keita Tamura, Nguyen van Thanh, Hideyuki Suzuki, Ryo Misaki, Toshiya Muranaka, Ninh Khac Ban, Kazuhito Fujiyama, Hikaru Seki

Plant Biotechnology　Vol. 35　No. 4　p. 341-348　2018/12/25　Research paper (scientific journal)

Delta-9 fatty acid desaturase overexpression enhanced lipid production and oleic acid content in Rhodosporidium toruloides for preferable yeast lipid production.

Yung-Yu Tsai, Takao Ohashi, Chih-Chan Wu, Dolgormaa Bataa, Ryo Misaki, Savitree Limtong, Kazuhito Fujiyama

Journal of bioscience and bioengineering　Vol. 127　No. 4　p. 430-440　2018/10　Research paper (scientific journal)

St6gal1 knockdown alters HBV life cycle in HepAD38 cells

Surya Agung Priyambada, Ryo Misaki, Toru Okamoto, Takao Ohashi, Keiji Ueda, Yoshiharu Matsuura, Kazuhito Fujiyama

Biochemical and Biophysical Research Communications　Vol. 503　No. 3　p. 1841-1847　2018/09　Research paper (scientific journal)

Isolation of a thermotolerant Rhodosporidium toruloides DMKU3-TK16 mutant and its fatty acid profile at high temperature.

Chih-Chan Wu, Yung-Yu Tsai, Takao Ohashi, Ryo Misaki, Savitree Limtong, Kazuhito Fujiyama

FEMS microbiology letters　Vol. 365　No. 21　2018/08　Research paper (scientific journal)

The production of human β-glucocerebrosidase in Nicotiana benthamiana root culture

Naphatsamon U, Ohashi T, Misaki R, Fujiyama K

Int J Mol Sci　2018/07　Research paper (scientific journal)

Enhancement of glycosylation by stable co-expression of two sialylation-related enzymes on Chinese hamster ovary cells

Nguyen TS, Misaki R, Ohashi T, Fujiyama K

J Biosci Bioeng　2018/07　Research paper (scientific journal)

Cell surface N-glycan alteration in HepAD38 cell lines expressing Hepatitis B virus

Surya Agung Priyambada, Ryo Misaki, Toru Okamoto, Yuta Okamoto, Takao Ohashi, Keiji Ueda, Yoshiharu Matsuura, Kazuhito Fujiyama

Virus Research　Vol. 238　p. 101-109　2017/06/15　Research paper (scientific journal)

Erratum to: Development of a sufficient and effective procedure for transformation of an oleaginous yeast, Rhodosporidium toruloides DMKU3-TK16 (Current Genetics, (2017), 63, 2, (359-371), 10.1007/s00294-016-0629-8)

Yung-Yu Tsai, Takao Ohashi, Takenori Kanazawa, Pirapan Polburee, Ryo Misaki, Savitree Limtong, Kazuhito Fujiyama

Current Genetics　Vol. 63　No. 2　2017/05/01　Research paper (scientific journal)

Fucosyltransferases produce N-glycans containing core l-galactose.

Hiroyuki Ohashi, Takao Ohashi, Hiroyuki Kajiura, Ryo Misaki, Shinichi Kitamura, Kazuhito Fujiyama

Biochemical and biophysical research communications　Vol. 483　No. 1　p. 658-663　2017/01　Research paper (scientific journal)

Core-fucosylation plays a pivotal role in hepatitis B pseudo virus infection: a possible implication for HBV glycotherapy

Shinji Takamatsu, Mayuka Shimomura, Yoshihiro Kamada, Haruka Maeda, Tomoaki Sobajima, Hayato Hikita, Masumi Iijima, Yuta Okamoto, Ryo Misaki, Kazuhito Fujiyama, Shushi Nagamori, Yoshikatsu Kanai, Tetsuo Takehara, Keiji Ueda, Shun'ichi Kuroda, Eiji Miyoshi

GLYCOBIOLOGY　Vol. 26　No. 11　p. 1180-1189　2016/11　Research paper (scientific journal)

The production of human glucocerebrosidase in glyco-engineered Nicotiana benthamiana plants.

Juthamard Limkul, Sayoko Iizuka, Yohei Sato, Ryo Misaki, Takao Ohashi, Toya Ohashi, Kazuhito Fujiyama

Plant biotechnology journal　Vol. 14　No. 8　p. 1682-94　2016/08　Research paper (scientific journal)

Development of a sufficient and effective procedure for transformation of an oleaginous yeast, Rhodosporidium toruloides DMKU3‑TK16

Tsai Y-Y, Ohashi T, Kanazawa T, Polburee P, Misaki R, Limtong S, Fujiyama K

Curr Genet　2016/07　Research paper (scientific journal)

Recombinant production and characterization of human anti-influenza virus monoclonal antibodies identified from hybridomas fused with human lymphocytes

Ryo Misaki, Natsuko Fukura, Hiroyuki Kajiura, Mayo Yasugi, Ritsuko Kubota-Koketsu, Tadahiro Sasaki, Masatoshi Momota, Ken ichiro Ono, Takao Ohashi, Kazuyoshi Ikuta, Kazuhito Fujiyama

Biologicals　Vol. 44　No. 5　p. 394-402　2016/07　Research paper (scientific journal)

Sialylation potentials of the silkworm, Bombyx mori; B. mori possesses an active alpha 2,6-sialyltransferase

Hiroyuki Kajiura, Yuichi Hamaguchi, Hiroki Mizushima, Ryo Misaki, Kazuhito Fujiyama

GLYCOBIOLOGY　Vol. 25　No. 12　p. 1441-1453　2015/12　Research paper (scientific journal)

The combination of plant translational enhancers and terminator increase the expression of human glucocerebrosidase in Nicotiana benthamiana plants

Juthamard Limkul, Ryo Misaki, Ko Kato, Kazuhito Fujiyama

Plant Science　Vol. 240　p. 41-49　2015/11/01　Research paper (scientific journal)

Substrate preference of citrus naringenin rhamnosyltransferases and their application to flavonoid glycoside production in fission yeast

Ohashi T, Hasegawa Y, Misaki R, Fujiyama K

Appl Microbiol Biotechnol　2015/10　Research paper (scientific journal)

Production of initial-stage eukaryotic N-glycan and its protein glycosylation in Escherichia coli

Srichaisupakit Akkaraphol, Ohashi Takao, Misaki Ryo, Fujiyama Kazuhito

Journal of bioscience and bioengineering　Vol. 119　No. 4　p. 399-405　2015/04　Research paper (scientific journal)

Antibody germline characterization of cross-neutralizing human IgGs against 4 serotypes of dengue virus.

Pannamthip Pitaksajjakul, Surachet Benjathummarak, Cholnatip Pipattanaboon, Waranya Wongwit, Tamaki Okabayashi, Motoki Kuhara, Ryo Misaki, Kazuhito Fujiyama, Pongrama Ramasoota

Biochemical and Biophysical Research Communicatioins　Vol. 446　No. 2　p. 475-480　2014/04　Research paper (scientific journal)

Cloning of a cDNA encoding the Gly m Bd 28K precursor and its vacuole transport in tobacco BY2 suspension-cultured cells.

Hitomi Yumioka-Ito, Ryo Misaki, Miyuki Yokoro, Makiko Suzuki, Hiromi Yamashita, Miki Hiemori-Kondo, Masumi Kimoto, Ko Kato, Kazuhito Fujiyama, Hideaki Tsuji

Journal of nutritional science and vitaminology　Vol. 60　No. 2　p. 129-39　2014　Research paper (scientific journal)

Emerging Antigenic Variants at the Antigenic Site Sb in Pandemic A(H1N1)2009 Influenza Virus in Japan Detected by a Human Monoclonal Antibody

M. Yasugi, R. Kubota-Koketsu, A. Yamashita, N. Kawashita, A. Du, R. Misaki, M. Kuhara, N. Boonsathorn, K. Fujiyama, Y. Okuno, T. Nakaya, K. Ikuta

PLoS One　Vol. 8　No. 10　2013/10　Research paper (scientific journal)

Dengue virus neutralization and antibody-ependetn enhancement activities of human monoclonal antibodies derived from dengue patiensts as acute phase of secondary infections.

Tadahiro Sasaki, Chayanee Setthapramote, Takeshi Kurosu, Mituhiro Nishimura, Azusa Asai, Tamaki Okabayashi, Magot Omokoko, Chonlatip Pipattanaboon, Pannnamthip Pitaksajjakul, Kriengsak, Limittikul, Arunee Subchareon, Panjaporn Chaichana, Tamaki Okabayashi, Itaru Hirai, Pornsawan Leaungwutiwong, Ryo Misaki, Kazuhiro Fujiyama, Ken-ichiro Ono, Yoshinobu Okuno, Pongrama Ramasoota, Kazuyoshi Ikuta

Antiviral Research　2013/06　Research paper (scientific journal)

Human monoclonal antibodies broadly neutralizing against influenza B virus.

Mayo Yasugi, Ritsuko Kubota-Koketsu, Akifumi Yamashita, Norihito Kawashita, Anariwa Du, Tadahiro Sasaki, Mitsuhiro Nishimura, Ryo Misaki, Motoki Kuhara, Naphatsawan Boonsathorn, Kazuhito Fujiyama, Yoshinobu Okuno, Takaaki Nakaya, Kazuyoshi Ikuta

PLoS pathogens　Vol. 9　No. 2　2013/02　Research paper (scientific journal)

Characterization of URA3 gene in oleaginous yeast Rhodosporidium toruloides DMKU3-TK16 for lipid engineering

Takenori Kanazawa, Takao Ohashi, Ayako Tomio, Ryo Misaki, Takeshi Bamba, Eiichiro Fukusaki, Limtong Savitree, Kazuhito Fujiyama

2012/10

N-terminal vacuolar sorting signal at the mouse antibody alters the N-linked glycosylation pattern in suspension-cultured tobacco BY2 cells

Misaki, R, Sakai, Y, Omasa, T, Fujiyama, K, Seki, T

J. Biosci. Bioeng.　Vol. 112　No. 5　p. 476-484　2011/11　Research paper (scientific journal)

Arabidopsis beta1,2-xylosyltransferase: Substrate specificity and participation in the plant-specific N-glycosylation pathway

Kajiura, H, Okamoto, T, Misaki, R, Matsuura, Y, Fujiyama, K

J. Biosci. Bioeng.　Vol. 113　No. 1　p. 48-54　2011/10　Research paper (scientific journal)

Intracellular phosphatidylserine is essential for retrograde membrane traffic through endosomes

Uchida, Y, Hasegawa, J, Chinnapen, D, Inoue, T, Okazaki, S, Kato, R, Wakatsuki, S, Misaki, R, Koike, M, Uchiyama, Y, Iemura, S, Natsume, T, Kuwahara, R, Nakagawa, T, Nishikawa, K, Mukai, K, Miyoshi, E, Taniguchi, N, Sheff, D, Lencer, W, Taguchi, T, Arai, H

Proc. Natl. Acad. Sci. U. S. A.　Vol. 108　No. 38　p. 15846-51　2011/09　Research paper (scientific journal)

Improved expression and characterization of recombinant human Golgi alpha1,2-mannosidase I isoforms (IA2 and IC) by Escherichia coli

Isoyama-Tanaka, J, Dohi, K, Misaki, R, Fujiyama, K

J. Biosci. Bioeng.　Vol. 112　No. 1　p. 14-19　2011/07　Research paper (scientific journal)

The recycling endosome protein Rab17 regulates melanocytic filopodia formation and melanosome trafficking

Beaumont, K.A, Hamilton, N.A, Moores, M.T, Brown, D.L, Ohbayashi, N, Caimcross, O, Cook, A.L, Smith, A.G, Misaki, R, Fukuda, M, Taguchi, T, Sturm, R.A, Stow, J

Traffic　Vol. 12　No. 5　p. 627-43　2011/05　Research paper (scientific journal)

Jack bean alpha-mannosidase digestion profile of hybrid-type N-glycans: Effect of reaction pH on substrate preference

Dohi, K, Isoyama-Tanaka, J, Misaki, R, Fujiyama, K

Biochimie　2011/04　Research paper (scientific journal)

Stable coexpression of two human sialylation enzymes in plant suspension-cultured tobacco cells

Kajiura, H, Misaki, R, Fujiyama, K, Seki, T

J. Biosci. Bioeng.　Vol. 111　No. 4　p. 471-477　2011/04　Research paper (scientific journal)

Palmitoylation pilots ras to recycling endosomes

Taguchi, T, Misaki, R

Small GTPases　Vol. 2　No. 2　p. 82-84　2011/03　Research paper (scientific journal)

Palmitoylated Ras proteins traffic through recycling endosomes to the plasma membrane during exocytosis

Misaki, R, Morimatsu, M, Uemura, T, Waguri, S, Miyoshi, E, Taniguchi, N, Matsuda, M, Taguchi, T

J. Cell Biol.　Vol. 191　No. 1　p. 23-9　2010/10　Research paper (scientific journal)

Phosphoinositide 3-kinase delta regulates membrane fission of Golgi carriers for selective cytokine secretion

Low, P.C, Misaki, R, Schroder, K, Stanley, A.C, Sweet, M.J, Teasdale, R.D, Vanhaesebroeck, B, Meunier, F.A, Taguchi, T, Stow, J.L

J. Cell Biol.　Vol. 190　No. 6　p. 1053-65　2010/09　Research paper (scientific journal)

Cloning and characterization of cytidine monophosphate-3-deoxy-D-manno-octulosonate synthetase from Arabidopsis thaliana

MISAKI Ryo, KAJIURA Hiroyuki, FUJII Kenji, FUJIYAMA Kazuhito, SEKI Tatsuji

Journal of bioscience and bioengineering　Vol. 108　No. 6　p. 527-529　2009/12　Research paper (scientific journal)

Change in glycosylation pattern with extension of endoplasmic reticulum retention signal sequence of mouse antibody produced by suspension-cultured tobacco BY2 cells.

Fujiyama K, Misaki R, Sakai Y, Omasa T, Seki T

Journal of bioscience and bioengineering　Vol. 107　No. 2　p. 165-172　2009/02　Research paper (scientific journal)

Rapt function requires palmitoylation and recycling endosome localization

Yukiko Uechi, Maitsetseg Bayarjargal, Masato Umikawa, Minoru Oshiro, Kimiko Takei, Yoshito Yamashiro, Tsuyoshi Asato, Shogo Endo, Ryo Misaki, Tomohiko Taguchi, Ken-ichi Kariya

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　Vol. 378　No. 4　p. 732-737　2009/01　Research paper (scientific journal)

Human RME-8 is involved in membrane trafficking through early endosomes.

Akemi Fujibayashi, Tomohiko Taguchi, Ryo Misaki, Masashi Ohtani, Naoshi Dohmae, Koji Takio, Masashi Yamada, Jianguo Gu, Megumi Yamakami, Mitsunori Fukuda, Satoshi Waguri, Yasuo Uchiyama, Tamotsu Yoshimori, Kiyotoshi Sekiguchi

Cell structure and function　Vol. 33　No. 1　p. 35-50　2008/02　Research paper (scientific journal)

Retrograde Transport of Glycolipid-Bound Toxins

Ryo Misaki, Tomohiko Taguchi

Experimental Glycoscience　p. 213-215　2008

Spatial segregation of degradation- and recycling-trafficking pathways in COS-1 cells.

Misaki, R, Nakagawa, T, Fukuda, M, Taniguchi, N, Taguchi, T

Biochem. Biophys. Res. Commun.　2007/06　Research paper (scientific journal)

Production of mouse monoclonal antibody with galactose-extended sugar chain by suspension cultured tobacco BY2 cells expressing human beta(1,4)-galactosyltransferase

Kazuhito Fujiyama, Aiko Furukawa, Atsuya Katsura, Ryo Misaki, Takeshi Omasa, Tatsuji Seki

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　Vol. 358　No. 1　p. 85-91　2007/04　Research paper (scientific journal)

In Planta Production of Immunogenic Poliovirus Peptide Using Tobacco Mosaic Virus-Based Vector System

Fujiyama Kazuhito, Saejung Wanida, Yanagihara Itaru, Nakado Junko, Misaki Ryo, Honda Takeshi, Watanabe Yuichiro, Seki Tatsuji

Journal of Fermentation Technology　Vol. 101　No. 5　p. 398-402　2006/03　Research paper (scientific journal)

N-linked glycan structures of a mouse monoclonal antibody produced from tobacco BY2 suspension-cultured cells

K Fujiyama, R Misaki, A Katsura, T Tanaka, A Furukawa, T Omasa, T Seki

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　Vol. 101　No. 3　p. 212-218　2006/03　Research paper (scientific journal)

Expression of human CMP-N-acetylneuraminic acid synthetase and CMP-sialic acid transporter in tobacco suspension-cultured cell

R Misaki, K Fujiyama, T Seki

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　Vol. 339　No. 4　p. 1184-1189　2006/01　Research paper (scientific journal)

Process development and economic evaluation of recombinant human lactoferrin expressed in rice grain

S Nandi, D Yalda, S Lu, Z Nikolov, R Misaki, K Fujiyama, N Huang

TRANSGENIC RESEARCH　Vol. 14　No. 3　p. 237-249　2005/06　Research paper (scientific journal)

N-linked glycan structures of human lactoferrin produced by transgenic rice

K Fujiyama, Y Sakai, R Misaki, Yanagihara, I, T Honda, H Anzai, T Seki

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY　Vol. 68　No. 12　p. 2565-2570　2004/12　Research paper (scientific journal)

Human N-acetylglucosaminyltransferase I. Expression in Escherichia coli as a soluble enzyme, and application as an immobilized enzyme for the chemoenzymatic synthesis of N-linked oligosaccharides

K Fujiyama, Y Ido, R Misaki, DG Moran, Yanagihara, I, T Honda, SI Nishimura, T Yoshida, T Seki

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　Vol. 92　No. 6　p. 569-574　2001/12　Research paper (scientific journal)