Cryo-electron tomography of the actin cytoskeleton and membrane organization during lamellipodia formation using optogenetics

Hironori Inaba, Tsuyoshi Imasaki, Kazuhiro Aoyama, Shogo Yoshihara, Hiroko Takazaki, Takayuki Kato, Hidemasa Goto, Kaoru Mitsuoka, Ryo Nitta, Takao Nakata

2024/08/14

Mechanism of ATP hydrolysis dependent rotation of bacterial ATP synthase.

Atsuki Nakano, Jun-Ichi Kishikawa, Kaoru Mitsuoka, Ken Yokoyama

Nature communications　Vol. 14　No. 1　p. 4090-4090　2023/07/10　Research paper (scientific journal)

Three structures of PSI-LHCI from Chlamydomonas reinhardtii suggest a resting state re-activated by ferredoxin.

Christoph Gerle, Yuko Misumi, Akihiro Kawamoto, Hideaki Tanaka, Hisako Kawai-Kubota, Ryutaro Tokutsu, Eunchul Kim, Dror Chorev, Kazuhiro Abe, Carol V Robinson, Kaoru Mitsuoka, Jun Minagawa, Genji Kurisu

Biochimica et biophysica acta. Bioenergetics　p. 148986-148986　2023/06/01　Research paper (scientific journal)

Dispersibility and surface properties of hydrocortisone-incorporated self-assemblies

Ward Wakileh, Nozomi Watanabe, Keishi Suga, Naoki Ikushima, Naoko Kajimura, Kaoru Mitsuoka, Yukihiro Okamoto, Hiroshi Umakoshi

Colloids and Surfaces A: Physicochemical and Engineering Aspects　Vol. 665　p. 131217-131217　2023/05　Research paper (scientific journal)

Rapid and Highly Stable Membrane Reconstitution by LAiR Enables the Study of Physiological Integral Membrane Protein Functions

Albert Godoy-Hernandez, Amer H. Asseri, Aiden J. Purugganan, Chimari Jiko, Carol de Ram, Holger Lill, Martin Pabst, Kaoru Mitsuoka, Christoph Gerle, Dirk Bald, Duncan G. G. McMillan

ACS Central Science　Vol. 9　No. 3　p. 494-507　2023/02/22　Research paper (scientific journal)

Cryo-EM analysis of V/A-ATPase intermediates reveals the transition of the ground-state structure to steady-state structures by sequential ATP binding.

Atsuko Nakanishi, Jun-Ichi Kishikawa, Kaoru Mitsuoka, Ken Yokoyama

The Journal of biological chemistry　p. 102884-102884　2023/01/07　Research paper (scientific journal)

Isolation and genomic analysis of a type IV pili-independent Thermus thermophilus phage, φMN1 from a Japanese hot spring

Masatada Tamakoshi, Atsushi Hijikata, Kei Yura, Kenshiro Oshima, Hidehiro Toh, Kaoru Mitsuoka, Tairo Oshima, Yoshitaka Bessho

The Journal of General and Applied Microbiology　2023　Research paper (scientific journal)

A novel capsid protein network allows the characteristic internal membrane structure of Marseilleviridae giant viruses.

Akane Chihara, Raymond N Burton-Smith, Naoko Kajimura, Kaoru Mitsuoka, Kenta Okamoto, Chihong Song, Kazuyoshi Murata

Scientific reports　Vol. 12　No. 1　p. 21428-21428　2022/12/11　Research paper (scientific journal)

Structural transition of the ground-state structure to steady-state structures by sequential binding of ATP to V/A-ATPase

Atsuko Nakanishi, Jun-ichi Kishikawa, Kaoru Mitsuoka, Ken Yokoyama

2022/10/05

Structural basis of unisite catalysis of bacterial F0F1-ATPase

Atsuki Nakano, Jun-ichi Kishikawa, Atsuko Nakanishi, Kaoru Mitsuoka, Ken Yokoyama

PNAS Nexus　Vol. 1　No. 3　2022/08/19　Research paper (scientific journal)

Dependence of the Core–Shell Structure on the Lipid Composition of Nanostructured Lipid Carriers: Implications for Drug Carrier Design

Ni’matul Izza, Nozomi Watanabe, Yukihiro Okamoto, Keishi Suga, Yusuf Wibisono, Naoko Kajimura, Kaoru Mitsuoka, Hiroshi Umakoshi

ACS Applied Nano Materials　Vol. 5　No. 7　p. 9958-9969　2022/07/22　Research paper (scientific journal)

CAMSAP2 organizes a γ-tubulin-independent microtubule nucleation centre through phase separation.

Tsuyoshi Imasaki, Satoshi Kikkawa, Shinsuke Niwa, Yumiko Saijo-Hamano, Hideki Shigematsu, Kazuhiro Aoyama, Kaoru Mitsuoka, Takahiro Shimizu, Mari Aoki, Ayako Sakamoto, Yuri Tomabechi, Naoki Sakai, Mikako Shirouzu, Shinya Taguchi, Yosuke Yamagishi, Tomiyoshi Setsu, Yoshiaki Sakihama, Eriko Nitta, Masatoshi Takeichi, Ryo Nitta

eLife　Vol. 11　2022/06/28　Research paper (scientific journal)

Structural snapshots of V/A-ATPase reveal the rotary catalytic mechanism of rotary ATPases

J. Kishikawa, A. Nakanishi, A. Nakano, S. Saeki, A. Furuta, T. Kato, K. Mistuoka, K. Yokoyama

Nature Communications　Vol. 13　No. 1　2022/03/08　Research paper (scientific journal)

A dynein-associated photoreceptor protein prevents ciliary acclimation to blue light

Osamu Kutomi, Ryosuke Yamamoto, Keiko Hirose, Katsutoshi Mizuno, Yuuhei Nakagiri, Hiroshi Imai, Akira Noga, Jagan Mohan Obbineni, Noemi Zimmermann, Masako Nakajima, Daisuke Shibata, Misa Shibata, Kogiku Shiba, Masaki Kita, Hideo Kigoshi, Yui Tanaka, Yuya Yamasaki, Yuma Asahina, Chihong Song, Mami Nomura, Mamoru Nomura, Ayako Nakajima, Mia Nakachi, Lixy Yamada, Shiori Nakazawa, Hitoshi Sawada, Kazuyoshi Murata, Kaoru Mitsuoka, Takashi Ishikawa, Ken-ichi Wakabayashi, Takahide Kon, Kazuo Inaba

Science Advances　Vol. 7　No. 9　2021/02/26　Research paper (scientific journal)

Mechanical inhibition of isolated Vo from V/A-ATPase for proton conductance.

Jun-Ichi Kishikawa, Atsuko Nakanishi, Aya Furuta, Takayuki Kato, Keiichi Namba, Masatada Tamakoshi, Kaoru Mitsuoka, Ken Yokoyama

eLife　Vol. 9　2020/07/08　Research paper (scientific journal)

Cryo-EM structures reveal translocational unfolding in the clostridial binary iota toxin complex.

Tomohito Yamada, Toru Yoshida, Akihiro Kawamoto, Kaoru Mitsuoka, Kenji Iwasaki, Hideaki Tsuge

Nature structural & molecular biology　Vol. 27　No. 3　p. 288-296　2020/03　Research paper (scientific journal)

Sequence- and seed-structure-dependent polymorphic fibrils of alpha-synuclein.

Tanaka G, Yamanaka T, Furukawa Y, Kajimura N, Mitsuoka K, Nukina N

Biochimica et biophysica acta. Molecular basis of disease　Vol. 1865　No. 6　p. 1410-1420　2019/06　Research paper (scientific journal)

Biochemical and morphological classification of disease-associated alpha-synuclein mutants aggregates.

Tanaka G, Yamanaka T, Furukawa Y, Kajimura N, Mitsuoka K, Nukina N

Biochemical and biophysical research communications　Vol. 508　No. 3　p. 729-734　2019/01　Research paper (scientific journal)

Cryo-EM studies of the rotary H+-ATPase/synthase from Thermus thermophilus.

Atsuko Nakanishi, Jun-Ichi Kishikawa, Kaoru Mitsuoka, Ken Yokoyama

Biophysics and physicobiology　Vol. 16　p. 140-146　2019　Research paper (scientific journal)

Cryo em structure of intact rotary H+-ATPase/synthase from Thermus thermophilus

Atsuko Nakanishi, Jun-Ichi Kishikawa, Masatada Tamakoshi, Kaoru Mitsuoka, Ken Yokoyama

Nature Communications　Vol. 9　No. 1　2018/12/01　Research paper (scientific journal)

Structural basis for cofilin binding and actin filament disassembly

Kotaro Tanaka, Shuichi Takeda, Kaoru Mitsuoka, Toshiro Oda, Chieko Kimura-Sakiyama, Yuichiro Maéda, Akihiro Narita

Nature Communications　Vol. 9　No. 1　2018/12/01　Research paper (scientific journal)

PB-13An Approach to Structural Analysis of a Small Membrane Protein KcsA by Cryo-electron Microscopy

Hiroko Takazaki, Hirofumi Shimizu, Naoko Kajimura, Kaoru Mitsuoka, Takuo Yasunaga

Microscopy　Vol. 66　No. suppl_1　p. i39-i39　2017/11/02　Research paper (scientific journal)

Effect of the nanoformulation of siRrNAa-lipid assemblies on their cellular uptake and immune stimulation

Kohei Kubota, Kohei Onishi, Kazuaki Sawaki, Tianshu Li, Kaoru Mitsuoka, Takaaki Sato, Shinji Takeoka

International Journal of Nanomedicine　Vol. 12　p. 5121-5133　2017/07/19　Research paper (scientific journal)

Optimization of STEM imaging conditions for cryo-tomography

Kazuhiro Aoyama, Kotaro Nagano, Kaoru Mitsuoka

MICROSCOPY　Vol. 66　No. 3　p. 212-216　2017/06　Research paper (scientific journal)

Chemical and structural characterization of a model Post-Termination Complex (PoTC) for the ribosome recycling reaction: Evidence for the release of the mRNA by RRF and EF-G

Nobuhiro Iwakura, Takeshi Yokoyama, Fabio Quaglia, Kaoru Mitsuoka, Kazuhiro Mio, Hideki Shigematsu, Mikako Shirouzu, Akira Kaji, Hideko Kaji

PLOS ONE　Vol. 12　No. 5　2017/04　Research paper (scientific journal)

Recent advances of single particle analysis by cryo-electron microscopy and its application to membrane proteins

Kaoru Mitsuoka, Christoph Gerle

Seikagaku　Vol. 88　No. 4　p. 532-536　2016

A pKa calculation of residues in a proton pump, bacteriorhodopsin, from structures determined by electron crystallography.

Mitsuoka K

Microscopy (Oxford, England)　Vol. 63 Suppl 1　p. i30-i31　2014/11　Research paper (international conference proceedings)

Structural analysis of the 26S proteasome by cryo-electron microscopy and Single-Particle Analysis.

Wang Z, Okuma Y, Kasuya D, Mitsuoka K, Saeki Y, Yasunaga T

Microscopy (Oxford, England)　Vol. 63 Suppl 1　2014/11　Research paper (international conference proceedings)

Asp-52 in Combination with Asp-398 Plays a Critical Role in ATP Hydrolysis of Chaperonin GroEL

Ayumi Koike-Takeshita, Kaoru Mitsuoka, Hideki Taguchi

JOURNAL OF BIOLOGICAL CHEMISTRY　Vol. 289　No. 43　p. 30005-11　2014/10　Research paper (scientific journal)

3P039 Structural analysis of the 26S proteasome by cryo-electron microscopy and Single-Particle Analysis(01B. Protein: Structure & Function,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

Wang Zhuo, Okuma Yasuo, Kasuya Daiske, Mitsuoka Kaoru, Saeki Yasushi, Yasunaga Takuo

Seibutsu Butsuri　Vol. 54　No. 1　2014

1P101 Fitting of a homology model to a projection map of aquaporin-3(03. Membrane proteins,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

Mitsuoka Kaoru

Seibutsu Butsuri　Vol. 54　No. 1　2014

Visualization of two distinct states of disassembly in the bacterial V-ATPase from Thermus thermophilus

Kazutoshi Tani, Christopher P. Arthur, Masatada Tamakoshi, Ken Yokoyama, Kaoru Mitsuoka, Yoshinori Fujiyoshi, Christoph Gerle

Journal of Electron Microscopy　Vol. 62　No. 4　p. 467-474　2013/08　Research paper (scientific journal)

Visualization of two distinct states of disassembly in the bacterial V-ATPase from Thermus thermophilus

Kazutoshi Tani, Christopher P. Arthur, Masatada Tamakoshi, Ken Yokoyama, Kaoru Mitsuoka, Yoshinori Fujiyoshi, Christoph Gerle

MICROSCOPY　Vol. 62　No. 4　p. 467-474　2013/08　Research paper (scientific journal)

Processing of electron diffraction patterns with the XDP program.

Mitsuoka K

Methods in molecular biology (Clifton, N.J.)　Vol. 955　p. 539-50　2013/04　Research paper (scientific journal)

2P010 Structural analysis of the 26S proteasome by cryo-electron microscopy and Single-Particle Analysis(01A. Protein: Structure,Poster)

Wang Zhuo, Okuma Yasuo, Kasuya Daiske, Mitsuoka Kaoru, Saeki Yasushi, Yasunaga Takuo

Seibutsu Butsuri　Vol. 53　No. 1　2013

3E1010 Structural analysis of the 26S proteasome by cryo-electron microscopy and Single-Particle Analysis(Proteins:Structure,Oral Presentation,The 50th Annual Meeting of the Biophysical Society of Japan)

Wang Zhuo, Okuma Yasuo, Kasuya Daisuke, Mitsuoka Kaoru, Saeki Yasushi, Takuo Yasunaga

Seibutsu Butsuri　Vol. 52　2012

Automatic recovery of missing amplitudes and phases in tilt-limited electron crystallography of two-dimensional crystals

Bryant R. Gipson, Daniel J. Masiel, Nigel D. Browning, John Spence, Kaoru Mitsuoka, Henning Stahlberg

PHYSICAL REVIEW E　Vol. 84　No. 1 Pt 1　2011/07　Research paper (scientific journal)

Report on the 50-year Anniversary Meeting of the Biophysical Society of Japan

MITSUOKA Kaoru

Seibutsu Butsuri　Vol. 51　No. 3　p. 146-147　2011/05/25

Genomic and proteomic characterization of the largeMyoviridaebacteriophage ϕTMA of the extreme thermophileThermus thermophilus

Masatada Tamakoshi, Aya Murakami, Motoki Sugisawa, Kenji Tsuneizumi, Shigeki Takeda, Toshihiko Saheki, Takashi Izumi, Toshihiko Akiba, Kaoru Mitsuoka, Hidehiro Toh, Atsushi Yamashita, Fumio Arisaka, Masahira Hattori, Tairo Oshima, Akihiko Yamagishi

Bacteriophage　Vol. 1　No. 3　p. 152-164　2011/05　Research paper (scientific journal)

Obtaining high-resolution images of biological macromolecules by using a cryo-electron microscope with a liquid-helium cooled stage

Kaoru Mitsuoka

MICRON　Vol. 42　No. 2　p. 100-6　2011/02

1G1312 3D structure of the 26S proteasome by high-resolution electron cryo-microscopy(Protein: Structure 1,The 49th Annual Meeting of the Biophysical Society of Japan)

Okuma Yasuo, Kasuya Daisuke, Mitsuoka Kaoru, Saeki Yasushi, Yasunaga Takuo

Seibutsu Butsuri　Vol. 51　2011

Helical image reconstruction of the outward-open human erythrocyte band 3 membrane domain in tubular crystals

Tomohiro Yamaguchi, Takashi Fujii, Yoshito Abe, Teruhisa Hirai, Dongchon Kang, Keiichi Namba, Naotaka Hamasaki, Kaoru Mitsuoka

JOURNAL OF STRUCTURAL BIOLOGY　Vol. 169　No. 3　p. 406-12　2010/03　Research paper (scientific journal)

2P089 Single particle analysis of the 26S proteasome by high-resolution electron cryo-microscopy and advanced image analysis(The 48th Annual Meeting of the Biophysical Society of Japan)

Okuma Yasuo, Kasuya Daisuke, Mitsuoka Kaoru, Saeki Yasushi, Yasunaga Takuo

Seibutsu Butsuri　Vol. 50　No. 2　2010

Structure of the Chaperonin-Substrate Complex

MITSUOKA Kaoru, KOIKE TAKESHITA Ayumi, TAGUCHI Hideki

Seibutsu Butsuri　Vol. 49　No. 6　p. 296-297　2009/12/25

Cryo-EM Structure of the Native GroEL-GroES Complex from Thermus thermophilus Encapsulating Substrate Inside the Cavity

Ryo Kanno, Ayumi Koike-Takeshita, Ken Yokoyama, Hideki Taguchi, Kaoru Mitsuoka

STRUCTURE　Vol. 17　No. 2　p. 287-93　2009/02　Research paper (scientific journal)

2P-001 High-resolution structural analysis of a water channel, AQP1, by electron crystallography(Protein:Structure, The 47th Annual Meeting of the Biophysical Society of Japan)

Mitsuoka Kaoru, Endou Hiroko, Kasuya Daisuke, Gyobu Nobuhiko

Seibutsu Butsuri　Vol. 49　2009

Structural basis for induced formation of the inflammatory mediator prostaglandin E-2

Caroline Jegerschold, Sven-Christian Paweizik, Pasi Purhonen, Priyaranian Bhakat, Karina Roxana Gheorghe, Nobuhiko Gyobu, Kaoru Mitsuoka, Ralf Morgenstern, Per-Johan Jakobsson, Hans Hebert

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　Vol. 105　No. 32　p. 11110-5　2008/08　Research paper (scientific journal)

Corrigendum to "Aquaporin-11 containing a divergent NPA motif has normal water channel activity" [Biochim. Biophys. Acta 1768 (2007) 688-693] (DOI:10.1016/j.bbamem.2006.11.005)

Kaya Yakata, Yoko Hiroaki, Kenichi Ishibashi, Eisei Sohara, Sei Sasaki, Kaoru Mitsuoka, Yoshinori Fujiyoshi

Biochimica et Biophysica Acta - Biomembranes　Vol. 1778　No. 6　2008/06　Research paper (scientific journal)

Neuromyelitis optica and anti-aquaporin-4 antibodies measured by an enzyme-linked immunosorbent assay

Sei Hayakawa, Masahiro Mori, Akiko Okuta, Akiko Kamegawa, Yoshinori Fujiyoshi, Yasumasa Yoshiyama, Kaoru Mitsuoka, Kenichi Ishibashi, Sei Sasaki, Takamichi Hattori, Satoshi Kuwabara

JOURNAL OF NEUROIMMUNOLOGY　Vol. 196　No. 1-2　p. 181-7　2008/05　Research paper (scientific journal)

3P-069 Development of Three-Dimensional Reconstruction System by integration of many processing tools(The 46th Annual Meeting of the Biophysical Society of Japan)

Nina Hitoshi, Taniguchi Kanae, Nishino Yuri, Iwasaki Kenji, Takagi Junichi, Miyazawa Atsuo, Mitsuoka Kaoru, Yasunaga Takuo

Seibutsu Butsuri　Vol. 48　2008

Dodecamer rotor ring defines H+/ATP ratio for ATP synthesis of prokaryotic V-ATPase from Thermus thermophilus

Masashi Toei, Christoph Gerle, Masahiro Nakano, Kazutoshi Tani, Nobuhiko Gyobu, Masatada Tamakoshi, Nobuhito Sone, Masasuke Yoshida, Yoshinori Fujiyoshi, Kaoru Mitsuoka, Ken Yokoyama

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　Vol. 104　No. 51　p. 20256-20261　2007/12　Research paper (scientific journal)

Simulation of charge effects on density maps obtained by high-resolution electron crystallography

Teruhisa Hirai, Kaoru Mitsuoka, Akinori Kidera, Yoshinori Fujiyoshi

JOURNAL OF ELECTRON MICROSCOPY　Vol. 56　No. 4　p. 131-40　2007/08　Research paper (scientific journal)

Aquaporin-11 containing a divergent NPA motif has normal water channel activity

Kaya Yakata, Yoko Hiroaki, Kenichi Ishibashi, Eisei Sohara, Sei Sasaki, Kaoru Mitsuoka, Yoshinori Fujiyoshi

Biochimica et Biophysica Acta - Biomembranes　Vol. 1768　No. 3　p. 688-693　2007/03　Research paper (scientific journal)

Aquaporin-11 containing a divergent NPA motif has normal water channel activity.

Yakata K, Hiroaki Y, Ishibashi K, Sohara E, Sasaki S, Mitsuoka K, Fujiyoshi Y

Biochimica et biophysica acta　Vol. 1768　No. 3　p. 688-93　2007/03　Research paper (scientific journal)

2P031 Structure of the GroEL-GroES-ADP-substrate protein complex from T. thermophilus by cryo-electron microscopy(Proteins-structure and structure-function relationship,Poster Presentations)

Kanno Ryo, Koike Ayumi, Yokoyama Ken, Taguchi Hideki, Mitsuoka Kaoru

Seibutsu Butsuri　Vol. 47　2007

2P331 Involvement of the molecular chaperone HtpG (Hsp90) in assembly and degradation of the supramolecular complex phycobilisome(Photobiology-photosynthesis, and vision and photoreception,Oral Presentations)

Okamoto Naoki, Mitsuoka Kaoru, Nakamoto Hitoshi

Seibutsu Butsuri　Vol. 47　2007

[Structure of a membrane protein in eicosanoid and glutathione metabolism by electron crystallography].

Gyobu N, Mitsuoka K

Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme　Vol. 52　No. 1　p. 44-49　2007/01

Structural basis for detoxification and oxidative stress protection in membranes

Peter J. Holm, Priyaranjan Bhakat, Caroline Jegerschold, Nobuhiko Gyobu, Kaoru Mitsuoka, Yoshinori Fujiyoshi, Ralf Morgenstern, Hans Hebert

JOURNAL OF MOLECULAR BIOLOGY　Vol. 360　No. 5　p. 934-45　2006/07　Research paper (scientific journal)

Two-dimensional crystallization and analysis of projection images of intact Thermus thermophilus V-ATPase

C Gerle, K Tani, K Yokoyama, M Tamakoshi, M Yoshida, Y Fujiyoshi, K Mitsuoka

JOURNAL OF STRUCTURAL BIOLOGY　Vol. 153　No. 2　p. 200-206　2006/02　Research paper (scientific journal)

2P155 Membrane structure of microsomal glutathione transferase 1 (MGST1) by electron crystallography(34. Membrane protein,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)

Holm Peter J, Bhakat Priyaranjan, Jegerschold Caroline, Gyobu Nobuhiko, Mitsuoka Kaoru, Fujiyoshi Yoshinori, Morgenstern Ralf, Hebert Hans

Seibutsu Butsuri　Vol. 46　No. 2　2006

Implications of the aquaporin-4 structure on array formation and cell adhesion

Y Hiroaki, K Tani, A Kamegawa, N Gyobu, K Nishikawa, H Suzuki, T Walz, S Sasaki, K Mitsuoka, K Kimura, A Mizoguchi, Y Fujiyoshi

JOURNAL OF MOLECULAR BIOLOGY　Vol. 355　No. 4　p. 628-39　2006/01　Research paper (scientific journal)

Electron tomography reveals diverse conformations of integrin alpha IIb beta 3 in the active state

K Iwasaki, K Mitsuoka, Y Fujiyoshi, Y Fujisawa, M Kikuchi, K Sekiguchi, T Yamada

JOURNAL OF STRUCTURAL BIOLOGY　Vol. 150　No. 3　p. 259-67　2005/06　Research paper (scientific journal)

Structural Analysis of Membrane Proteins from the Two-Dimensional Crystals by Cryo-Electron Microscopy

MITSUOKA Kaoru

X-RAYS　Vol. 47　No. 1　p. 44-49　2005/02/24

[Three-dimensional structural analysis of biological molecules by cryo-electron microscopy].

Mitsuoka K, Fujiyoshi Y

Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme　Vol. 49　No. 11 Suppl　p. 1794-1799　2004/08

Improved specimen preparation for cryo-electron microscopy using a symmetric carbon sandwich technique

N Gyobu, K Tani, Y Hiroaki, A Kamegawa, K Mitsuoka, Y Fujiyoshi

JOURNAL OF STRUCTURAL BIOLOGY　Vol. 146　No. 3　p. 325-33　2004/06　Research paper (scientific journal)

An image evaluation system coupled with auto-TEM

N. Nakamura, Y. Fujiyoshi, K. Mitsuoka, K. Murata, T. Shinkawa

Microscopy and Microanalysis　Vol. 9　No. 2　p. 1038-1039　2003　Research paper (international conference proceedings)

Roles of Met-34, Cys-64, and Arg-75 in the assembly of human connexin 26 - Implication for key amino acid residues for channel formation and function

A Oshima, T Doi, K Mitsuoka, S Maeda, Y Fujiyoshi

JOURNAL OF BIOLOGICAL CHEMISTRY　Vol. 278　No. 3　p. 1807-16　2003/01　Research paper (scientific journal)

Structure and function of water channels

Y Fujiyoshi, K Mitsuoka, BL de Groot, A Philippsen, H Grubmuller, P Agre, A Engel

CURRENT OPINION IN STRUCTURAL BIOLOGY　Vol. 12　No. 4　p. 509-515　2002/08

Structure and function of water channels

Y Fujiyoshi, K Mitsuoka, BL de Groot, A Philippsen, H Grubmuller, P Agre, A Engel

CURRENT OPINION IN STRUCTURAL BIOLOGY　Vol. 12　No. 4　p. 509-515　2002/08

A new technique to co-localise membrane proteins with Homer/vesl

Y Hiroaki, K Nishikawa, K Mitsuoka, T Tachibana, K Sobue, T Doi, Y Fujiyoshi

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　Vol. 295　No. 3　p. 756-65　2002/07　Research paper (scientific journal)

A new technique to co-localise membrane proteins with Homer/vesl

Y Hiroaki, K Nishikawa, K Mitsuoka, T Tachibana, K Sobue, T Doi, Y Fujiyoshi

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　Vol. 295　No. 3　p. 756-765　2002/07　Research paper (scientific journal)

Crystal structure of the homer 1 family conserved region reveals the interaction between the EVH1 domain and own proline-rich motif

K Irie, T Nakatsu, K Mitsuoka, A Miyazawa, K Sobue, Y Hiroaki, T Doi, Y Fujiyoshi, H Kato

JOURNAL OF MOLECULAR BIOLOGY　Vol. 318　No. 4　p. 1117-26　2002/05　Research paper (scientific journal)

Two-dimensional crystals: a powerful approach to assess structure, function and dynamics of membrane proteins

H Stahlberg, D Fotiadis, S Scheuring, H Remigy, T Braun, K Mitsuoka, Y Fujiyoshi, A Engel

FEBS LETTERS　Vol. 504　No. 3　p. 166-172　2001/08　Research paper (scientific journal)

Structures of membrane proteins determined by electron crystallography and their relationship to the function

Mitsuoka K.

Seibutsu Butsuri　Vol. 41　2001

Two-dimensional crystallization of the complex between photoactivated rhodopsin and transducin

Nishimura S., Gyobu N., Kandori H., Furutani Y., Mitsuoka K., Fujiyoshi Y., Shichida Y.

Seibutsu Butsuri　Vol. 41　2001

The three-dimensional map of microsomal glutathione transferase 1 at 6 angstrom resolution

Schmidt-Krey, I, K Mitsuoka, T Hirai, K Murata, Y Cheng, Y Fujiyoshi, R Morgenstern, H Hebert

EMBO JOURNAL　Vol. 19　No. 23　p. 6311-6316　2000/12　Research paper (scientific journal)

Structural determinants of water permeation through aquaporin-1

K Murata, K Mitsuoka, T Hirai, T Walz, P Agre, JB Heymann, A Engel, Y Fujiyoshi

NATURE　Vol. 407　No. 6804　p. 599-605　2000/10　Research paper (scientific journal)

Structural Analysis of Protein

MITSUOKA Kaoru

kenbikyo　Vol. 35　No. 2　p. 156-157　2000/07/31

The fold of human aquaporin 1

BL de Groot, JB Heymann, A Engel, K Mitsuoka, Y Fujiyoshi, H Grubmuller

JOURNAL OF MOLECULAR BIOLOGY　Vol. 300　No. 4　p. 987-994　2000/07　Research paper (scientific journal)

The 11 Å resolution projection map of Na+/K+-ATPase calculated by application of single particle analysis to two-dimensional crystal images

Tahara Yoshikazu, Oshima Atsunori, Hirai Teruhisa, Mitsuoka Kaoru, Fujiyoshi Yoshinori, Hayashi Yutaro

Journal of Electron Microscopy　Vol. 49　No. 4　p. 583-587　2000　Research paper (scientific journal)

The 11 angstrom resolution projection structure of Na+/K+-ATPase

Y Tahara, A Oshima, T Hirai, K Mitsuoka, Y Fujiyoshi, Y Hayashi

NA/K-ATPASE AND RELATED ATPASES　Vol. 1207　p. 187-191　2000　Research paper (international conference proceedings)

The three-dimensional map of microsomal glutathione transferase 1 at 3 Å resolution.

EMBO J.　Vol. 19, 6311-6316　2000

The structure of aquaporin-1 at 4.5-angstrom resolution reveals short alpha-helices in the center of the monomer

K Mitsuoka, K Murata, T Walz, T Hirai, P Agre, JB Heymann, A Engel, Y Fujiyoshi

JOURNAL OF STRUCTURAL BIOLOGY　Vol. 128　No. 1　p. 34-43　1999/12　Research paper (scientific journal)

Structure of Bacteriorhodopsin Based on Electron Crystallography at 3.0 A Resolution

MITSUOKA Kaoru, HIRAI Teruhisa

Seibutsu Butsuri　Vol. 39　No. 3　p. 148-153　1999/05

The projection structure of the membrane protein microsomal glutathione transferase at 3 angstrom resolution as determined from two-dimensional hexagonal crystals

Schmidt-Krey, I, K Murata, T Hirai, K Mitsuoka, YF Cheng, R Morgenstern, Y Fujiyoshi, H Hebert

JOURNAL OF MOLECULAR BIOLOGY　Vol. 288　No. 2　p. 243-253　1999/04　Research paper (scientific journal)

Image analysis of electron micrographs from two-dimensional crystals to build an atomic model

MITSUOKA Kaoru

kenbikyo　Vol. 34　No. 1　p. 44-48　1999/03/31

The structure of bacteriorhodopsin at 3.0 angstrom resolution based on electron crystallography: Implication of the charge distribution

K Mitsuoka, T Hirai, K Murata, A Miyazawa, A Kidera, Y Kimura, Y Fujiyoshi

JOURNAL OF MOLECULAR BIOLOGY　Vol. 286　No. 3　p. 861-882　1999/02　Research paper (scientific journal)

Influence of various nucleotides on the in situ crystallization of Ca2+-ATPase

Yoko Hiroaki, Kaoru Mitsuoka, Yifan Cheng, Hidekazu Hiroaki, Yoshinori Fujiyoshi

Biochimica et Biophysica Acta - Biomembranes　Vol. 1415　No. 2　p. 361-368　1999/01/08　Research paper (scientific journal)

Improvement of the Na^+/K^+-ATPhase projection map by the single particle analysis of two dimansional crystals

Oshima A., Mitsuoka K., Hirai T., Fujiyoshi Y., Tahara Y., Hayashi Y.

Seibutsu Butsuri　Vol. 39　1999

Trehalose embedding technique for high-resolution electron crystallography: application to structural study on bacteriorhoposin

T Hirai, K Murata, K Mitsuoka, Y Kimura, Y Fujiyoshi

JOURNAL OF ELECTRON MICROSCOPY　Vol. 48　No. 5　p. 653-658　1999　Research paper (scientific journal)

Expression, purification, and reconstitution of receptor for pituitary adenylate cyclase-activating polypeptide - Large-scale purification of a functionally active G protein-coupled receptor produced in Sf9 insect cells

T Ohtaki, K Ogi, Y Masuda, K Mitsuoka, Y Fujiyoshi, C Kitada, H Sawada, H Onda, M Fujino

JOURNAL OF BIOLOGICAL CHEMISTRY　Vol. 273　No. 25　p. 15464-15473　1998/06　Research paper (scientific journal)

Projection map of the reaction center-light harvesting 1 complex from Rhodopseudomonas viridis at 10 Å resolution

Ikuko Ikeda-Yamasaki, Takayuki Odahara, Kaoru Mitsuoka, Yoshinori Fujiyoshi, Kazuyoshi Murata

FEBS Letters　Vol. 425　No. 3　p. 505-508　1998/04/03　Research paper (scientific journal)

The 3.0 angstrom projection structure of microsomal glutathione transferase as determined by electron crystallography of p2(1)2(1)2 two-dimensional crystals

H Hebert, SchmidtKrey, I, R Morgenstern, K Murata, T Hirai, K Mitsuoka, Y Fujiyoshi

JOURNAL OF MOLECULAR BIOLOGY　Vol. 271　No. 5　p. 751-758　1997/09　Research paper (scientific journal)

Surface of bacteriorhodopsin revealed by high-resolution electron crystallography

Y Kimura, DG Vassylyev, A Miyazawa, A Kidera, M Matsushima, K Mitsuoka, K Murata, T Hirai, Y Fujiyoshi

NATURE　Vol. 389　No. 6647　p. 206-211　1997/09　Research paper (scientific journal)

Electron and atomic force microscopy of membrane proteins

JB Heymann, DJ Muller, K Mitsuoka, A Engel

CURRENT OPINION IN STRUCTURAL BIOLOGY　Vol. 7　No. 4　p. 543-549　1997/08　Research paper (scientific journal)

The three-dimensional structure of aquaporin-1

T Walz, T Hirai, K Murata, JB Heymann, K Mitsuoka, Y Fujiyoshi, BL Smith, P Agre, A Engel

NATURE　Vol. 387　No. 6633　p. 624-627　1997/06　Research paper (scientific journal)

Examination of the LeafScan 45, a line-illuminating micro-densitometer, for its use in electron crystallography

K Mitsuoka, K Murata, Y Kimura, K Namba, Y Fujiyoshi

ULTRAMICROSCOPY　Vol. 68　No. 2　p. 109-121　1997/06　Research paper (scientific journal)

電子線結晶学による膜蛋白質の構造解析

光岡 薫, 藤吉 好則

日本結晶学会誌　Vol. 39　p. 9-10　1997

High Resolution Structure of Bacteriorhodopsin Determined by Electron Crystallography

Yoshiaki Kimura, Dmitry G. Vassylyev, Atsuo Miyazawa, Akinori Kidera, Masaaki Matsushima, Kaoru Mitsuoka, Kazuyoshi Murata, Teruhisa Hirai, Yoshinori Fujiyoshi

Photochemistry and Photobiology　Vol. 66　No. 6　p. 764-767　1997　Research paper (scientific journal)