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Imai Hiroshi
今井　洋
Imai Hiroshi
今井　洋
Graduate School of Science　Department of Biological Sciences, Assistant Professor
hiroshi.imai bio.sci.osaka-u.ac.jp

keyword Glutamate dehydrogenase，cryo-EM，microtubule，dynein

Research History 5

  1. 2017/04 - Present
    Osaka University　Graduate School of Science Department of Biological Sciences　Assistant Professor

  2. 2015/04 - 2017/03
    Chuo University　Faculty of Science and Engineering Department of Biological Sciences　Assistant Professor

  3. 2009/04 - 2015/03
    University of Leeds, United Kingdom　Astbury Centre for Structural Molecular Biology　Research Fellow

  4. 2005/04 - 2009/03
    Japan Science and Technology Agency　Researcher

  5. 2003/04 - 2005/03
    RIKEN　Harima Institute　Contract researcher

Education 1

  1. University of Tokyo　Graduate School of Science　Department of Biological Sciences

    1997/04 - 2003/03

Professional Memberships 3

  1. American society for cell biology

  2. Zoological Society of Japan

  3. THE BIOPHYSICAL SOCIETY OF JAPAN

Research Areas 3

  1. Life sciences / Cell biology /

  2. Life sciences / Biophysics /

  3. Life sciences / Structural biochemistry /

Awards 1

  1. Alpbach Motor Meeting Award

    IMAI Hiroshi　15th Alpbach Motors Workshop, Myosin and Muscle, and other Motors.　2016/03

Papers 9

  1. Heterodimeric Ciliary Dynein f/I1 Adopts a Distinctive Structure, Providing Insight Into the Autoinhibitory Mechanism Common to the Dynein Family

    Yici Lei, Akira Fukunaga, Hiroshi Imai, Ryosuke Yamamoto, Rieko Shimo‐Kon, Shinji Kamimura, Kaoru Mitsuoka, Takako Kato‐Minoura, Toshiki Yagi, Takahide Kon

    Cytoskeleton　2025/01/04　Research paper (scientific journal)

    Publisher: Wiley

  2. A dynein-associated photoreceptor protein prevents ciliary acclimation to blue light

    Osamu Kutomi, Ryosuke Yamamoto, Keiko Hirose, Katsutoshi Mizuno, Yuuhei Nakagiri, Hiroshi Imai, Akira Noga, Mohan Obbineni, Noemi Zimmermann, Masako Nakajima, Daisuke Shibata, Misa Shibata, Kogiku Shiba, Masaki Kita, Hideo Kigoshi, Yui Tanaka, Yuya Yamasaki, Yuma Asahina, Chihong Song, Mami Nomura, Mamoru Nomura, Ayako Nakajima, Mia Nakachi, Lixy Yamada, Shiori Nakazawa, Hitoshi Sawada, Kazuyoshi Murata, Kaoru Mitsuoka, Takashi Ishikawa, Ken-ichi Wakabayashi, Takahide Kon, Kazuo Inaba

    Science Advances: Vol. 7, no. 9, eabf3621 DOI: 10.1126/sciadv.abf3621　Vol. 7　No. 9　2021/02　Research paper (scientific journal)

  3. GTP-dependent formation of straight oligomers leads to nucleation of microtubules

    Rie Ayukawa (*), Seigo Iwata (*), Hiroshi Imai (*), Shinji Kamimura, Masahito Hayashi, Kien Xuan Ngo, Itsushi Minoura, Seiichi Uchimura, Tsukasa Makino, Mikako Shirouzu, Hideki Shigematsu, Ken Sekimoto, Benoît Gigant, Etsuko Muto (*These authors contributed equally)

    J Cell Biol 220 (4): e202007033.　Vol. 220　No. 4　2021/02　Research paper (scientific journal)

    Publisher: Rockefeller University Press

  4. Development of phase contrast/dark-field microscopy with scanning laser illumination.

    T. Sato, T. Shirane, Y. Wada, H. Imai, S. Kamimura

    J. Inst. Sci. Eng. Chuo Univ.　2019

  5. Direct observation shows superposition and large scale flexibility within cytoplasmic dynein motors moving along microtubules

    Hiroshi Imai, Tomohiro Shima, Kazuo Sutoh, Matthew L. Walker, Peter J. Knight, Takahide Kon, Stan A. Burgess

    NATURE COMMUNICATIONS　Vol. 6　2015/09　Research paper (scientific journal)

  6. Dynactin 3D Structure: Implications for Assembly and Dynein Binding

    Hiroshi Imai, Akihiro Narita, Yuichiro Maeda, Trina A. Schroer

    JOURNAL OF MOLECULAR BIOLOGY　Vol. 426　No. 19　p. 3262-3271　2014/09　Research paper (scientific journal)

  7. mNUDC is required for plus-end-directed transport of cytoplasmic dynein and dynactins by kinesin-1

    Masami Yamada, Shiori Toba, Takako Takitoh, Yuko Yoshida, Daisuke Mori, Takeshi Nakamura, Atsuko H. Iwane, Toshio Yanagida, Hiroshi Imai, Li-yuan Yu-Lee, Trina Schroer, Anthony Wynshaw-Boris, Shinji Hirotsune

    EMBO JOURNAL　Vol. 29　No. 3　p. 517-531　2010/02　Research paper (scientific journal)

  8. Two-dimensional averaged images of the dynactin complex revealed by single particle analysis

    H Imai, A Narita, TA Schroer, Y Maeda

    JOURNAL OF MOLECULAR BIOLOGY　Vol. 359　No. 4　p. 833-839　2006/06　Research paper (scientific journal)

  9. Effects of trypsin-digested outer-arm dynein fragments on the velocity of microtubule sliding in elastase-digested flagellar axonemes

    H Imai, C Shingyoji

    CELL STRUCTURE AND FUNCTION　Vol. 28　No. 1　p. 71-86　2003/02　Research paper (scientific journal)

Misc. 5

  1. Two different linker conformations of the cytoplasmic dynein motor domain in the presence of ADP

    H. Imai, R. Kanazawa, R. Shimo-Kon, T. Kon

    Molecular Biology of the Cell　Vol. 35　2023/12　Research paper, summary (international conference)

  2. Effects of high hydrostatic pressure to swimming sea urchin sperm flagella.

    H. Imai, M. Nishiyama, Y. Kaminoe, Y. Harada, T. Kon, S. Kamimura

    Molecular Biology of the Cell　Vol. 35　2023/12　Research paper, summary (international conference)

  3. Dynamics of the Spontaneous Nucleation of Microtubules

    Hiroshi IMAI, Etsuko MUTO

    Seibutsu Butsuri　Vol. 63　No. 6　p. 299-302　2023/12　Article, review, commentary, editorial, etc. (scientific journal)

    Publisher: Biophysical Society of Japan

  4. GTP-Dependent Formation of Straight Oligomers Leads to Nucleation of Microtubules

    Etsuko Muto, Rie Ayukawa, Seigo Iwata, Hiroshi Imai, Shinji Kamimura, Ken Sekimoto, Gigant Benoit

    BIOPHYSICAL JOURNAL　Vol. 120　No. 3　p. 12A-12A　2021/02　Research paper, summary (international conference)

  5. 動物細胞に共通する細胞内の輸送システム

    今井 洋, 昆 隆英

    生産と技術　Vol. 70　No. 4　p. 73-76　2018/10　Rapid communication, short report, research note, etc. (scientific journal)

Publications 1

  1. Handbook of dynein (Second edition)

    Editor, Keiko Hirose. Authors, Anthony J. Roberts, Katerina Toropova, and Hiroshi Imai.

    Jenny Stanford Publishing　2019

    ISBN: 9789814800013

Presentations 29

  1. 微小管格子の多様性の構造基盤

    前嶋捷久, 今井洋, 成田哲博, 昆隆英

    生体運動研究合同班会議 2025　2025/01/06

  2. 繊毛ダイニン IDA-fの不活性構造から活性化への分子メカニズム

    雷宜慈, 今井洋, 山本遼介, 下理恵子, 八木俊樹, 宮田知子, 牧野文信, 難波啓一, 昆隆英

    生体運動研究合同班会議 2025　2025/01/06

  3. 繊毛ダイニン・細胞質ダイニン・IFT ダイニンの構造的共通性と多様性

    雷宜慈, 今井洋, 山本遼介, 下理恵子, 宮田知子, 梶村直子, 八木俊樹, 上村慎治, 光岡薫, 難波啓一, 昆隆英

    生体運動研究合同班会議2024　2024/01/06

  4. 繊毛微小管内部タンパク質 Rib43a は正常な繊毛運動と繊毛軸糸の物理的安定性に重要である

    丹羽蘭丸, 山本遼介, 今井洋, 柴小菊, 八木俊樹, 稲葉一男, 昆隆英

    生体運動研究合同班会議2024　2024/01/06

  5. Effects of high hydrostatic pressure to swimming sea urchin sperm flagella.

    H. Imai, M. Nishiyama, Y. Kaminoe, Y. Harada, T. Kon, S. Kamimura

    2023 American Society for Cell Biology Annual Meeting　2023/12/05

  6. Two different linker conformations of the cytoplasmic dynein motor domain in the presence of ADP

    H. Imai, R. Kanazawa, R. Shimo-Kon, T. Kon

    2023 American Society for Cell Biology Annual Meeting　2023/12/03

  7. 14 to 16 protofilament GMPCPP-microtubules structures revealed by cryo-EM

    Toshihisa Maeshima, Reina Okamoto, Hiroshi Imai, Naoko Kajimura, Kaoru Mitsuoka, Mika Hirose, Takayuki Kato, Akihiro Narita, Takahide Kon

    The 61st Annual Meeting of the Biophysical Society of Japan　2023/11/14

  8. New insights into the mechanism of dynein force generation

    Hiroshi Imai, Takahide Kon

    The 49th Naito conference on frontiers of microtubule and its-related motors　2023/07/06

  9. 電子顕微鏡単粒子解析法による繊毛ダイニン新規構造の解明

    雷宜慈, 今井洋, 山本遼介, 重兼拓実, 下理恵子, 上村慎治, 八木俊樹, 梶村直子, 光岡薫, 昆隆英

    生体運動合同班会議　2023/01/09

  10. ダイニンメカニズム解明の新展開 理研シンポジウム

    重兼拓実, 今井洋, 昆隆英

    第11回分子モーター討論会　2022/11/14

  11. Development of a new method to measure dissociation constant of filamentous protein complexes by electron microscopy

    Masato Watanabe1, Hiroshi Imai1, Tomoko Miyata3, Fumiaki Makino3,4, Etsuko Muto5, Christoph Gerle2, Kaoru Mitsuoka6, Genji Kurisu2, Keiichi Namba3, Takahide Kon1 (1Grad. Sch. Sci.,Osaka Univ., 2IPR, Osaka Univ., 3Grad. Sch. Frontier Biosci., Osaka Univ., 4JEOL, 5Chuo Univ, 6Res.Ctr. UHVEM, Osaka Univ.)

    The 59th Annual Meeting of the Biophysical Society of Japan　2021/11/27

  12. Novel isolated ciliary dynein structure revealed by negative stain EM

    Yici Lei1, Hiroshi Imai1, Ryosuke Yamamoto1, Rieko Shimo1, Shinji Kamimura2, Toshiki Yagi3, Naoko Kajimura5, Mika Hirose4, Takayuki Kato4, Kaoru Mitsuoka5, Takahide Kon1 (1Dept. Biol. Sci.,Grad. Sch. Of Sci., Osaka Univ., 2Dept. Biol. Sci., Chuo Univ., 3Dept. Life Sci., Prefect. Univ. Hiroshima, 4Inst. Of Protein Res., Osaka Univ., 5Res. Ctr. UHVEM, Osaka Univ.)

    The 59th Annual Meeting of the Biophysical Society of Japan　2021/11/26

  13. Effects of high hydrostatic pressure on intracellular Ca2+ concentration of sea urchin swimming live sperm

    Hiroshi Imai, Masayoshi Nishiyama, Yumiko Kaminoe, Yoshie Harada, Takahide Kon, Shinji Kamimura, Grad. Sch. Sci, Osaka Univ, Dept. Biol. Sci, Chuo Univ, Dep. Phys, Kindai Univ, IPR, Osaka U

    2020/09/16

  14. How do GTP-tubulins nucleate microtubules? (2)Lateral association of oligomers.

    Seigo Iwata, Rie Ayukawa, Hiroshi Imai, Shinji Kamimura, Ken Sekimoto, Benoit Gigant, Etsuko Muto, CBS, RIKEN, Chuo Univ, Paris Univ, Paris-Saclay Univ, Osaka Uni

    2020/09/16

  15. Release mechanism of the shutdown state of cytoplasmic dynein revealed by electron microscopy

    Kazuki Iwasaki, Yui Kurume, Hiroshi Imai, Shinji Kamimura, Rieko Shimo-kon, Ryosuke Yamamoto, Takahide Kon, Grad. Sch. Sci, Osaka Univ, Dept.Biol.Sci, huo Un

    2020/09/16

  16. How do GTP-tubulins nucleate microtubules? (1)Formation of straight oligomers.

    Rie Ayukawa, Seigo Iwata, Hiroshi Imai, Shinji Kamimura, Masahito Hayashi, Kien Ngo, Itsushi Minoura, Seiichi Uchimura, Tsukasa Makino, Hideki Shigematsu, Ken Sekimoto, Benoit Gigant, Etsuko Muto, RIKEN CBS, Chuo Univ, Paris Univ, Paris-Saclay Univ, Osaka Univ, RIKEN BDR

    2020/09/16

  17. In situ localization of a novel ciliary-dynein subunit having a light-sensing domain revealed by cryo-electron tomogra- phy

    Ryosuke Yamamoto, Yuhei Nakagiri, Osamu Kutomi, Hi, roshi Imai, Chihong Song, Kazuyoshi Murata, Kaoru, Mitsuoka, Ken-ichi Wakabayashi, Takashi Ishikawa, Ka- zuo Inaba, Takahide Ko, Graduate School of Science, Osaka University, niversi, ty of Yamanashi, National Institute for, Physiological Sci- ences, Research Center for, Ultra-High Voltage Electron, Microscopy, Osaka University, Tokyo Institute of Technol- ogy, Paul Scherrer Institute, University of Tsukuba

    2020/05/27

  18. Dynamic changes of tubulin dimer configurations on a scale of sub-second revealed by high flux X-ray fiber diffraction

    Shinji Kamimura, Hiroshi Imai, Toshiki Yagi, Hiroyuki Iwamoto, Dept. Biol. Sci, Chuo Univ, Grad. Sch. Sci, Osaka Univ, Dept. Life Sci, Prefect, Univ. Hiroshima, SPring, JASRI

    2019/09/25

  19. Structural/functional analyses on MOT7, a novel light chain of ciliary dynein f/I1

    Ryosuke Yamamoto, Yuuhei Nakagiri, Osamu Kutomi, Hiroshi Imai, Chihong Song, Kazuyoshi Murata, Ken-ichi Wakabayashi, Takashi Ishikawa, Kazuo Inaba, Takahide Kon, Osaka Univ, Univ. of Yamanashi, NIPS, Tokyo Tech, PSI, Univ. of Tsukub

    2019/09/25

  20. A newly developed negative stain EM method for protein complexes at high protein concentration

    Hiroshi Imai, Takayuki Kato, Gerle Christoph, Etsuko Muto, Kaoru Mitsuoka, Genji Kurisu, Keiichi Namba, Takahide Kon, Grad. Sch. Sci, Osaka Univ, Grad. Sch, Frontier Biosci, Osaka Univ, IPR, Osaka Univ, CBS, RIKEN, Res. Ctr. UVHEM, Osaka Uni

    2019/09/24

  21. 高タンパク質濃度条件下のタンパク質複合体の構造を観察するための新規のネガティブ染色電子顕 微鏡法

    今井洋(阪大, 院理, 中桐 侑平, Gerle,C(阪大, 白研, 武藤悦子, 栗栖源嗣, 昆隆英

    2019年生体運動合同班会議　2019/01/04

  22. Cryo-EM observation of stepping patterns of cytoplasmic dynein on microtubules with new freezing conditions

    Riko Kanazawa, Hiroshi Imai, Takuma Shioi, Rieko Shimo, Ryousuke Yamamoto, Kaoru Mitsuoka, Takahide Kon, Dep. Boil, Grad. Sch. of, Sci, Osaka Univ, Res. Ctr. UVHEM, Univ. Osaka

    The 56th Annual Meeting of the Biophysical Society of Japan　2018/09/16

  23. Novel intermediate structures of cytoplasmic dynein between shutdown and active states

    Takuma Shioi, Akira Fukunaga, Rieko Shimo, Ryousuke Yamamoto, Hiroshi Imai, Takahide Kon, Dep. Biol. Sci, Grad. Sch. of Sci, Osaka Uni

    The 56th Annual Meeting of the Biophysical Society of Japan　2018/09/16

  24. Journey to the centre of the cell: Cryo-EM observation of a stepping protein on its track.

    IMAI Hiroshi

    国際蛋白研セミナー　2018/05/09

  25. 細胞内輸送の動力源の一つ・蛋白質「ダイニン」の不活性化状態と活 性化状態の構造解析

    今井 洋

    大阪大学超高圧電子顕微鏡センター医学・生物系共同利用研究報 告会　2018/03/01

  26. How does cytoplasmic dynein stepping along microtubules look like?

    今井 洋, 昆 隆英

    DYNEIN 2017 International Workshop　2017/10/30

  27. Direct observation of cytoplasmic dynein motors stepping along microtubules.

    H. Imai, T. Shima, K. Sutoh, M.L. Walker, P.J. Knight, T. Kon, S.A. Burgess

    The 2014 ASCB/IFCB meeting　2014/12/10

  28. Direct observation by cryo-EM of cytoplasmic dynein motors stepping along microtubules.

    H. Imai, T. Shima, K. Sutoh, M.L. Walker, P.J. Knight, T. Kon, S.A. Burgess

    Gordon Research Conference (Muscle and Molecular Motors)　2014/07/07

  29. Structure and the function of the dynactin complex

    今井 洋

    Gordon Research Conference (3DEM)　2006

Social Activities 6

  • 大阪大学における高校生訪問の受け入れ（大阪府立高津高校）透過型電子顕微鏡による微小管の観察体験

    大阪大学大学院理学研究科

    2022/11/07 - 2022/11/07

  • 大阪大学における高校生訪問の受け入れ（大阪府立高津高校）透過型電子顕微鏡による微小管の観察体験

    大阪大学大学院理学研究科

    2021/11/08 - 2021/11/08

  • 大阪大学における高校生訪問の受け入れ（大阪府立高津高校）透過型電子顕微鏡による微小管の観察体験

    大阪大学大学院理学研究科

    2020/11/09 - 2020/11/09

  • 高校生への実習, 大阪大学 Z-sce 分子生物学実習 学びを生きる力に変える 科学 的キャリア教育 「ジャイアントインパクト」

    大阪大学大学院理学研究科

    2018/08/28 -

  • 高校生への実習, 大阪大学 Z-sce 分子生物学実習 学びを生きる力に変える 科学 的キャリア教育 「ジャイアントインパクト」

    大阪大学大学院理学研究科

    2017/12 -

  • 高校生への実習, 大阪大学 Z-sce 分子生物学実習 学びを生きる力に変える 科学 的キャリア教育 「ジャイアントインパクト」

    大阪大学大学院理学研究科

    2017/08 -

Other 1

  1. 文部科学省検定「生物」の高等学校の教科書に研究内容が掲載されています

    2018/04 - Present