Model-based control system design to manage process parameters in mammalian cell culture for biopharmaceutical manufacturing.

Ayumu Sakaki, Tetsushi Namatame, Makoto Nakaya, Takeshi Omasa

Biotechnology and bioengineering　Vol. 121　No. 2　p. 605-617　2024/02　Research paper (scientific journal)

Construction of a novel kinetic model for the production process of a CVA6 VLP vaccine in CHO cells.

Zhou Xing, Thao Bich Nguyen, Guirong Kanai-Bai, Noriko Yamano-Adachi, Takeshi Omasa

Cytotechnology　Vol. 76　No. 1　p. 69-83　2024/02　Research paper (scientific journal)

Effects of genome instability of parental CHO cell clones on chromosome number distribution and recombinant protein production in parent-derived subclones.

Noriko Yamano-Adachi, Hirofumi Hata, Yuto Nakanishi, Takeshi Omasa

Journal of bioscience and bioengineering　Vol. 137　No. 1　p. 54-63　2024/01　Research paper (scientific journal)

Enhancement of recombinant adeno-associated virus activity by improved stoichiometry and homogeneity of capsid protein assembly.

Takayuki Onishi, Michika Nonaka, Takahiro Maruno, Yuki Yamaguchi, Mitsuko Fukuhara, Tetsuo Torisu, Masaharu Maeda, Susan Abbatiello, Anisha Haris, Keith Richardson, Kevin Giles, Steve Preece, Noriko Yamano-Adachi, Takeshi Omasa, Susumu Uchiyama

Molecular therapy. Methods & clinical development　Vol. 31　p. 101142-101142　2023/12/14　Research paper (scientific journal)

Synthesis of Human Antibodies Against HBsAg in Newly Established Chinese Hamster Lung (CHL-YN) Cell Line

Meutia Diva Hakim, Noriko Yamano- Adachi, Takeshi Omasa, Marselina Irasonia Tan, Lia Dewi Juliawaty, Ernawati Arifin Giri-Rachman

Journal of Mathematical and Fundamental Sciences　Vol. 54　No. 3　p. 290-301　2023/05/07　Research paper (scientific journal)

AFOB会長に就任して

大政健史

Vol. 101　No. 3　p. 111-111　2023/03

Artificial induction of chromosome aneuploidy in CHO cells alters their function as host cells.

Noriko Yamano-Adachi, Yuto Nakanishi, Wataru Tanaka, YuanShan Lai, Masahiro Yamazaki, Laura Zenner, Hirofumi Hata, Takeshi Omasa

Biotechnology and Bioengineering　Vol. 120　No. 3　p. 659-673　2023/03　Research paper (scientific journal)

培養でのものつくりについて

大政健史

Food & Food Ingredients Journal of Japan (FFIジャーナル)　Vol. 228　No. 2　p. 107-109　2023/02　Research paper (scientific journal)

10 g/Lの高生産を支える抗体医薬の生産技術

大政健史, 山野-足立範子

実験医学　Vol. 40　No. 20　p. 3198-3205　2022/12　Research paper (scientific journal)

Cellulase production on easy-to-handle solid media containing agricultural waste and its application for enzymatic hydrolysis of cellulosic biomass

Chizuru Sasaki, Kazuo Matsuura, Takeshi Omasa

Biomass Conversion and Biorefinery　2022/11/10　Research paper (scientific journal)

Effect of co-overexpression of the cargo receptor ERGIC-53/MCFD2 on antibody production and intracellular IgG secretion in recombinant Chinese hamster ovary cells.

Yutaka Kirimoto, Noriko Yamano-Adachi, Yuichi Koga, Takeshi Omasa

Journal of Bioscience and Bioengineering　2022/08/11　Research paper (scientific journal)

[Research and Development of Manufacturing of Biologics-the Activities of Manufacturing Association of Biologics].

Takeshi Omasa

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan　Vol. 142　No. 7　p. 723-729　2022/07

Development of Manufacturing Technology for Gene-therapy

Vol. 53　No. 2　p. 91-97　2022/05　Research paper (scientific journal)

Secretion of a low-molecular-weight species of endogenous GRP94 devoid of the KDEL motif during endoplasmic reticulum stress in Chinese hamster ovary cells.

Andrew Samy, Noriko Yamano-Adachi, Yuichi Koga, Takeshi Omasa

Traffic (Copenhagen, Denmark)　Vol. 22　No. 12　p. 425-438　2021/09/18　Research paper (scientific journal)

Production of monoclonal shark-derived immunoglobulin new antigen receptor antibodies using Chinese hamster ovary cell expression system

Hajime Enatsu, Nako Okamoto, Yoshiki Nomura, Masayoshi Onitsuka, Noriko Yamano-Adachi, Yuichi Koga, Takeshi Omasa

Journal of Bioscience and Bioengineering　Vol. 132　No. 3　p. 302-309　2021/09　Research paper (scientific journal)

バイオ医薬品の連続生産に関するPoints to Consider

奥平 真一, 櫻井 京子, 竹田 寛, 松田 嘉弘, 寶田 哲仁, 近藤 耕平, 髙橋 正史, 石井 隆聖, 内田 和久, 李 仁義, 和田 和洋, 小林 有己, 清水 理恵, 加藤 泰史, 倉嶋 秀樹, 畑山 勝浩, 應田 豊雄, 鳥飼 祐介, 苅谷 金弥, 塚本 次郎, 大倉 寛也, 渡辺 直人, 村井 活史, 針金谷 尚人, 平澤 竜太郎, 本郷 智子, 尾山 和信, 時枝 養之, 太田 康勝, 柴田 瑞世, 篠永 英樹, 柴 陽一郎, 清水 美明, 吉野 武, 堀内 貴之, 石川 芳光, 大江 正剛, 河﨑 忠好, 柴田 寛子, 日向 昌司, 本田 真也, 山本 修一, 村上 聖, 大政 健史, 石井 明子

GMPとバリデーション　Vol. 23　No. 1　p. 13-22　2021/06　Research paper (scientific journal)

Violacein improves recombinant IgG production by controlling the cell cycle of Chinese hamster ovary cells

Masahide Kido, Hideaki Idogaki, Kouji Nishikawa, Takeshi Omasa

Cytotechnology　Vol. 73　No. 3　p. 319-332　2021/06　Research paper (scientific journal)

Single‐cell transcriptome analyses reveal heterogeneity in suspension cultures and clonal markers of CHO‐K1 cells

Norichika Ogata, Akio Nishimura, Tomoko Matsuda, Michi Kubota, Takeshi Omasa

Biotechnology and Bioengineering　Vol. 118　No. 2　p. 944-951　2021/02　Research paper (scientific journal)

Establishment of fast-growing serum-free immortalised cells from Chinese hamster lung tissues for biopharmaceutical production.

Noriko Yamano-Adachi, Rintaro Arishima, Sukwattananipaat Puriwat, Takeshi Omasa

Scientific reports　Vol. 10　No. 1　p. 17612-17612　2020/10/19　Research paper (scientific journal)

Development of a scale-up strategy for Chinese hamster ovary cell culture processes using the kL a ratio as a direct indicator of gas stripping conditions.

Tomohiro Doi, Hideyuki Kajihara, Yasuo Chuman, Shinobu Kuwae, Takashi Kaminagayoshi, Takeshi Omasa

Biotechnology progress　Vol. 36　No. 5　2020/09　Research paper (scientific journal)

Effect of the disulfide isomerase PDIa4 on the antibody production of Chinese hamster ovary cells.

Kei Komatsu, Kento Kumon, Mayuno Arita, Masayoshi Onitsuka, Takeshi Omasa, Masafumi Yohda

Journal of bioscience and bioengineering　2020/08/30　Research paper (scientific journal)

Low-concentration staurosporine improves recombinant antibody productivity in Chinese hamster ovary cells without inducing cell death.

Masahide Kido, Hideaki Idogaki, Kouji Nishikawa, Takeshi Omasa

Journal of bioscience and bioengineering　2020/08/12　Research paper (scientific journal)

Screening of new cell cycle suppressive compounds from marine-derived microorganisms in Chinese hamster ovary cells.

Masahide Kido, Hideaki Idogaki, Kouji Nishikawa, Kana Motoishi, Takeshi Omasa

Journal of bioscience and bioengineering　Vol. 130　No. 1　p. 106-113　2020/07　Research paper (scientific journal)

Improvement of Intracellular Traffic System by Overexpression of KDEL Receptor 1 in Antibody-Producing CHO Cells.

Andrew Samy, Kohei Kaneyoshi, Takeshi Omasa

Biotechnology journal　Vol. 15　No. 6　2020/06　Research paper (scientific journal)

Build-up functionalization of anti-EGFR × anti-CD3 bispecific diabodies by integrating high-affinity mutants and functional molecular formats.

Ryutaro Asano, Katsuhiro Hosokawa, Shintaro Taki, Shota Konno, Ippei Shimomura, Hiromi Ogata, Mai Okada, Kyoko Arai, Masayoshi Onitsuka, Takeshi Omasa, Takeshi Nakanishi, Mitsuo Umetsu, Izumi Kumagai

Scientific reports　Vol. 10　No. 1　p. 4913-4913　2020/03/18　Research paper (scientific journal)

Characterization of Chinese hamster ovary cells with disparate chromosome numbers: Reduction of the amount of mRNA relative to total protein.

Noriko Yamano-Adachi, Norichika Ogata, Sho Tanaka, Masayoshi Onitsuka, Takeshi Omasa

Journal of bioscience and bioengineering　Vol. 129　No. 1　p. 121-128　2020/01　Research paper (scientific journal)

Characterization of Chinese hamster lung (CHL) cell lines, focusing on gene expression analysis

Rintaro Arishima, Noriko Yamano-Adachi, Yuichi Koga, Takeshi Omasa

2019/11

Engineering of the endoplasmic reticulum machinery in recombinant CHO cells for improvement antibody productivity

Andrew Samy, Jana Frank, Takeshi Omasa

2019/11

A study on soluble endoplasmic reticulum chaperones and their retention machinery in recombinant CHO cells

Andrew Samy, Jana Frank, Takeshi Omasa

2019/09

Use of aphidicolin to increase production of biologics in Chinese hamster ovary (CHO) cells

Jana Frank, Noriko Yamano-Adachi, Takeshi Omasa

2019/09

Application of calcium chloride in medium supplementation and flocculation owing to increase IgG high poductivity in CHO cells

Waritthorn Thanakarn, Jana Frank, Noriko Yamano-Adachi, Takeshi Omasa

2019/09

The Fab portion of immunoglobulin G contributes to its binding to Fcγ receptor III.

Rina Yogo, Yuki Yamaguchi, Hiroki Watanabe, Hirokazu Yagi, Tadashi Satoh, Mahito Nakanishi, Masayoshi Onitsuka, Takeshi Omasa, Mari Shimada, Takahiro Maruno, Tetsuo Torisu, Shio Watanabe, Daisuke Higo, Takayuki Uchihashi, Saeko Yanaka, Susumu Uchiyama, Koichi Kato

Scientific reports　Vol. 9　No. 1　p. 11957-11957　2019/08/16　Research paper (scientific journal)

Secretory leakage of IgG1 aggregates from recombinant Chinese hamster ovary cells.

Masayoshi Onitsuka, Yukinori Kadoya, Takeshi Omasa

Journal of bioscience and bioengineering　Vol. 127　No. 6　p. 752-757　2019/06　Research paper (scientific journal)

Secretion analysis of intracellular "difficult-to-express" immunoglobulin G (IgG) in Chinese hamster ovary (CHO) cells.

Kohei Kaneyoshi, Kouki Kuroda, Keiji Uchiyama, Masayoshi Onitsuka, Noriko Yamano-Adachi, Yuichi Koga, Takeshi Omasa

Cytotechnology　Vol. 71　No. 1　p. 305-316　2019/02　Research paper (scientific journal)

Analysis of the immunoglobulin G (IgG) secretion efficiency in recombinant Chinese hamster ovary (CHO) cells by using Citrine-fusion IgG.

Kohei Kaneyoshi, Noriko Yamano-Adachi, Yuichi Koga, Keiji Uchiyama, Takeshi Omasa

Cytotechnology　Vol. 71　No. 1　p. 193-207　2019/02　Research paper (scientific journal)

Analysis of intracellular IgG secretion in Chinese hamster ovary cells to improve IgG production.

Kohei Kaneyoshi, Keiji Uchiyama, Masayoshi Onitsuka, Noriko Yamano, Yuichi Koga, Takeshi Omasa

Journal of bioscience and bioengineering　Vol. 127　No. 1　p. 107-113　2019/01　Research paper (scientific journal)

EGCG improves recombinant protein productivity in Chinese hamster ovary cell cultures via cell proliferation control.

Noriko Yamano, Takeshi Omasa

Cytotechnology　Vol. 70　No. 6　p. 1697-1706　2018/12　Research paper (scientific journal)

Cell-cycle inhibitor aphidicolin has lasting effects on recombinant gene expression in Chinese hamster ovary cell lines

Jana Frank, Noriko Yamano, Takeshi Omasa

2018/11

Quantitative analysis of intracellular secretion of recombinant antibodies in CHO cells for establishment of high producers

Kouki Kuroda, Kohei Kaneyoshi, Noriko Yamano, Yuichi Koga, Takeshi Omasa

2018/11

DNA motif analysis of Chinese hamster ovary chromosome 1 for targeted gene insertion

Jana Frank, Noriko Yamano, Takeshi Omasa

2018/11

Characterization of CHO cells with disparate chromosome numbers and induction of artificial aneuploidy cells

Noriko Yamano, Norichika Ogata, Yuan Shan Lai, Sho Tanaka, Wataru Tanaka, Yuichi Koga, Takeshi Omasa

2018/11

Genome sequence comparison between Chinese hamster ovary (CHO) DG44 cells and mouse using end sequences of CHO BAC clones based on BAC-FISH results.

Shuichi Kimura, Takeshi Omasa

Cytotechnology　Vol. 70　No. 5　p. 1399-1407　2018/10　Research paper (scientific journal)

The Healing Effect of Human Milk Fat Globule-EGF Factor 8 Protein (MFG-E8) in A Rat Model of Parkinson's Disease.

Yoshiki Nakashima, Chika Miyagi-Shiohira, Hirofumi Noguchi, Takeshi Omasa

Brain sciences　Vol. 8　No. 9　2018/08/31　Research paper (scientific journal)

Atorvastatin Inhibits the HIF1α-PPAR Axis, Which Is Essential for Maintaining the Function of Human Induced Pluripotent Stem Cells.

Yoshiki Nakashima, Chika Miyagi-Shiohira, Hirofumi Noguchi, Takeshi Omasa

Molecular therapy : the journal of the American Society of Gene Therapy　Vol. 26　No. 7　p. 1715-1734　2018/07/05　Research paper (scientific journal)

Development of hyper osmotic resistant CHO host cells for enhanced antibody production.

Yasuharu Kamachi, Takeshi Omasa

Journal of bioscience and bioengineering　Vol. 125　No. 4　p. 470-478　2018/04　Research paper (scientific journal)

Enhanced IgG1 production by overexpression of nuclear factor kappa B inhibitor zeta (NFKBIZ) in Chinese hamster ovary cells.

Masayoshi Onitsuka, Yukie Kinoshita, Akitoshi Nishizawa, Tomomi Tsutsui, Takeshi Omasa

Cytotechnology　Vol. 70　No. 2　p. 675-685　2018/04　Research paper (scientific journal)

The enhancement of antibody concentration and achievement of high cell density CHO cell cultivation by adding nucleoside.

Yasuhiro Takagi, Takuya Kikuchi, Ryuta Wada, Takeshi Omasa

Cytotechnology　Vol. 69　No. 3　p. 511-521　2017/06　Research paper (scientific journal)

Improved recombinant antibody production by CHO cells using a production enhancer DNA element with repeated transgene integration at a predetermined chromosomal site.

Yoshinori Kawabe, Takanori Inao, Shodai Komatsu, Guan Huang, Akira Ito, Takeshi Omasa, Masamichi Kamihira

Journal of bioscience and bioengineering　Vol. 123　No. 3　p. 390-397　2017/03　Research paper (scientific journal)

Lengthening of high-yield production levels of monoclonal antibody-producing Chinese hamster ovary cells by downregulation of breast cancer 1

Rima Matsuyama, Noriko Yamano, Namiko Kawamura, Takeshi Omasa

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　Vol. 123　No. 3　p. 382-389　2017/03　Research paper (scientific journal)

Identification of regulatory motifs in the CHO genome for stable monoclonal antibody production

Yasuhiro Takagi, Tomomi Yamazaki, Kenji Masuda, Shigeaki Nishii, Bunsei Kawakami, Takeshi Omasa

Cytotechnology　Vol. 69　No. 3　p. 451-460　2016/06　Research paper (scientific journal)

Increased recombinant protein production owing to expanded opportunities for vector integration in high chromosome number Chinese hamster ovary cells

Noriko Yamano, Mai Takahashi, Seyed Mohammad Ali Haghparast, Masayoshi Onitsuka, Toshitaka Kumamoto, Jana Frank, Takeshi Omasa

Journal of Bioscience and Bioengineering　Vol. 122　No. 2　p. 226-231　2016/02

Metabolic analysis of antibody porducing Chinese hamster ovary cell culture suppressing antibody aggregation

Takeshi Omasa, Masayoshi Onitsuka

Metabolic Engineering 11　p. 163-174　2016　Research paper (international conference proceedings)

Rapid Colorimetric Antibody Detection Using a Dual-function Peptide Probe for Silver Nanoparticle Aggregation and Antibody Recognition

Mina Okochi, Tomohiro Kamiya, Takeshi Omasa, Masayoshi Tanaka, Hiroyuki Honda

ANALYTICAL SCIENCES　Vol. 32　No. 1　p. 93-97　2016/01　Research paper (scientific journal)

Metabolic analysis of antibody producing CHO cell culture under different stresses conditions

Md. Bahadur Badsha, Hiroyuki Kurata, Masayoshi Onitsuka, Takushi Oga, Takeshi Omasa

Journal of Bioscience and Bioengineering　Vol. 122　No. 1　p. 117-124　2016/01

Improved Photobactericidal Activity of Ultraviolet-A Light in Combination with Isomerizable p-Coumaric Acid Derivatives

Akihiro Shirai, Masato Kajiura, Kyohei Matsumura, Takeshi Omasa

BIOCONTROL SCIENCE　Vol. 20　No. 4　p. 231-238　2015/12　Research paper (scientific journal)

Effect of NaCl and Trehalose Condition on Antibody Producing CHO Cell Culture

Md. Bahadur Badsha, Hiroyuki Kurata, Masayoshi Onitsuka, Takushi Oga, Takeshi Omasa

Proceedings of Asian Congress on Biotechnology 2015 (ACB2015)　2015/11/15　Research paper (international conference proceedings)

Synergistic Photobactericidal Activity Based on Ultraviolet-A Irradiation and Ferulic Acid Derivatives

Akihiro Shirai, Masato Kajiura, Takeshi Omasa

PHOTOCHEMISTRY AND PHOTOBIOLOGY　Vol. 91　No. 6　p. 1422-1428　2015/11　Research paper (scientific journal)

Efficient enrichment of high-producing recombinant Chinese hamster ovary cells for monoclonal antibody by flow cytometry

Takeshi Okumura, Kenji Masuda, Kazuhiko Watanabe, Kenji Miyadai, Koichi Nonaka, Masayuki Yabuta, Takeshi Omasa

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　Vol. 120　No. 3　p. 340-346　2015/09　Research paper (scientific journal)

Optimization of cell line development in the GS-CHO expression system using a high-throughput, single cell-based clone selection system

Tsuyoshi Nakamura, Takeshi Omasa

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　Vol. 120　No. 3　p. 323-329　2015/09　Research paper (scientific journal)

Rapid evaluation of N-glycosylation status of antibodies with chemiluminescent lectin-binding assay

Masayoshi Onitsuka, Takeshi Omasa

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　Vol. 120　No. 1　p. 107-110　2015/07　Research paper (scientific journal)

Improved gene amplification by cell-cycle engineering combined with the Cre-loxP system in Chinese hamster ovary cells

Rima Matsuyama, Tomomi Tsutsui, Kyoung Ho Lee, Masayoshi Onitsuka, Takeshi Omasa

Journal of Bioscience and Bioengineering　Vol. 120　No. 6　p. 701-708　2015/06　Research paper (scientific journal)

Approaches to quality risk management when using single use systems in the manufacture of biologics

Akiko Ishii-Watabe, Akihiko Hirose, Noriko Katori, Norikata Hashii, Susumu Arai, Hirotoshi Awatsu, Akira Eiza, Yoshiaki Hara, Hideshi Hattori, Tomomi Inoue, Tetsuya Isono, Masahiro Iwakura, Daisuke Kajihara, Nobuo Kasahara, Hiroyuki Matsuda, Sei Murakami, Taishiro Nakagawa, Takehiro Okumura, Takeshi Omasa, Shinya Takuma, Iyo Terashima, Masayoshi Tsukahara, Maiko Tsutsui, Takahiro Yano, Nana Kawasaki

AAPS PharmSciTech　Vol. 16　No. 5　p. 993-1001　2015/05　Research paper (scientific journal)

Effect of Polyphenols on Reactive Oxygen Species Production and Cell Growth of Human Dermal Fibroblasts after Irradiation with Ultraviolet-A Light

Akihiro Shirai, Masayoshi Onitsuka, Hideaki Maseda, Takeshi Omasa

BIOCONTROL SCIENCE　Vol. 20　No. 1　p. 27-33　2015/03　Research paper (scientific journal)

The osteoblastic differentiation ability of human dedifferentiated fat cells is higher than that of adipose stem cells from the buccal fat pad

Naotaka Kishimoto, Yoshihiro Momota, Yoshiya Hashimoto, Shinichi Tatsumi, Kayoko Ando, Takeshi Omasa, Junichiro Kotani

CLINICAL ORAL INVESTIGATIONS　Vol. 18　No. 8　p. 1893-1901　2014/11　Research paper (scientific journal)

Neuraminidase gene homology contributes to the protective activity of influenza vaccines prepared from the influenza virus library

Ahmad M. Haredy, Hiroshi Yamada, Yoshihiro Sakoda, Masatoshi Okamatsu, Naoki Yamamoto, Takeshi Omasa, Yasuko Mori, Hiroshi Kida, Shigefumi Okamoto, Yoshinobu Okuno, Koichi Yamanishi

JOURNAL OF GENERAL VIROLOGY　Vol. 95　p. 2365-2371　2014/11　Research paper (scientific journal)

Rearranging the domain order of a diabody-based IgG-like bispecific antibody enhances its antitumor activity and improves its degradation resistance and pharmacokinetics

Ryutaro Asano, Ippei Shimomura, Shota Konno, Akiko Ito, Yosuke Masakari, Ryota Orimo, Shintaro Taki, Kyoko Arai, Hiromi Ogata, Mai Okada, Shozo Furumoto, Masayoshi Onitsuka, Takeshi Omasa, Hiroki Hayashi, Yu Katayose, Michiaki Unno, Toshio Kudo, Mitsuo Umetsu, Izumi Kumagai

MABS　Vol. 6　No. 5　p. 1243-1254　2014/09　Research paper (scientific journal)

Synergistic antimicrobial activity based on the combined use of a gemini-quaternary ammonium compound and ultraviolet-A light

Akihiro Shirai, Mutsumi Aihara, Akira Takahashi, Hideaki Maseda, Takeshi Omasa

Journal of Photochemistry and Photobiology B: Biology　Vol. 130　p. 226-233　2014/01/05　Research paper (scientific journal)

Glycosylation analysis of an aggregated antibody produced by Chinese hamster ovary cells in bioreactor culture

Masayoshi Onitsuka, Akira Kawaguchi, Ryutaro Asano, Izumi Kumagai, Kohsuke Honda, Hisao Ohtake, Takeshi Omasa

Journal of Bioscience and Bioengineering　Vol. 117　No. 5　p. 639-644　2014　Research paper (scientific journal)

Trehalose suppresses antibody aggregation during the culture of Chinese hamster ovary cells

Masayoshi Onitsuka, Miki Tatsuzawa, Ryutaro Asano, Izumi Kumagai, Akihiro Shirai, Hideaki Maseda, Takeshi Omasa

Journal of Bioscience and Bioengineering　Vol. 117　No. 5　p. 632-638　2014　Research paper (scientific journal)

Overexpression of mutant cell division cycle 25 homolog B (CDC25B) enhances the efficiency of selection in Chinese hamster ovary cells

Kyoung Ho Lee, Tomomi Tsutsui, Kohsuke Honda, Hisao Ohtake, Takeshi Omasa

Cytotechnology　Vol. 65　No. 6　p. 1017-1026　2013/12　Research paper (international conference proceedings)

Synthesis of Thiazole Derivatives and Evaluation of Their Antiamoebic Activity and Cytotoxicity

Akihiro Shirai, Toshiyuki Endo, Hideaki Maseda, Takeshi Omasa

BIOCONTROL SCIENCE　Vol. 18　No. 4　p. 183-191　2013/12　Research paper (scientific journal)

Generation of high-producing cell lines by overexpression of cell division cycle 25 homolog A in Chinese hamster ovary cells

Kyoung Ho Lee, Tomomi Tsutsui, Kohsuke Honda, Ryutaro Asano, Izumi Kumagai, Hisao Ohtake, Takeshi Omasa

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　Vol. 116　No. 6　p. 754-760　2013/12　Research paper (scientific journal)

Improved antibody production in Chinese hamster ovary cells by ATF4 overexpression

Ahmad M. Haredy, Akitoshi Nishizawa, Kohsuke Honda, Tomoshi Ohya, Hisao Ohtake, Takeshi Omasa

Cytotechnology　Vol. 65　No. 6　p. 993-1002　2013/12　Research paper (international conference proceedings)

Rapid construction of transgene-amplified CHO cell lines by cell cycle checkpoint engineering

Kyoung Ho Lee, Masayoshi Onitsuka, Kohsuke Honda, Hisao Ohtake, Takeshi Omasa

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY　Vol. 97　No. 13　p. 5731-5741　2013/07　Research paper (scientific journal)

An MDCK cell culture-derived formalin-inactivated influenza virus whole-virion vaccine from an influenza virus library confers cross-protective immunity by intranasal administration in mice

Ahmad M. Haredy, Nobuyuki Takenaka, Hiroshi Yamada, Yoshihiro Sakoda, Masatoshi Okamatsu, Naoki Yamamoto, Takeshi Omasa, Hisao Ohtake, Yasuko Mori, Hiroshi Kida, Koichi Yamanishi, Shigefumi Okamoto

Clinical and Vaccine Immunology　Vol. 20　No. 7　p. 998-1007　2013/07　Research paper (scientific journal)

Synthesis and Biological Activity of Thiazolyl-Acetic Acid Derivatives as Possible Antimicrobial Agents

Akihiro Shirai, Yasuko Fumoto, Tomoaki Shouno, Hideaki Maseda, Takeshi Omasa

BIOCONTROL SCIENCE　Vol. 18　No. 2　p. 59-73　2013/06　Research paper (scientific journal)

Two routes of MexS-MexT-mediated regulation of MexEF-OprN and MexAB-OprM efflux pump expression in Pseudomonas aeruginosa

Maki Uwate, Yu-ki Ichise, Akihiro Shirai, Takeshi Omasa, Taiji Nakae, Hideaki Maseda

MICROBIOLOGY AND IMMUNOLOGY　Vol. 57　No. 4　p. 263-272　2013/04　Research paper (scientific journal)

Development of a continuous bioconversion system using a thermophilic whole-cell biocatalyst

Pham Huynh Ninh, Kohsuke Honda, Yukako Yokohigashi, Kenji Okano, Takeshi Omasa, Hisao Ohtakea

Applied and Environmental Microbiology　Vol. 79　No. 6　p. 1996-2001　2013/03　Research paper (scientific journal)

Dedifferentiated fat cells differentiate into osteoblasts in titanium fiber mesh

Naotaka Kishimoto, Yoshihiro Momota, Yoshiya Hashimoto, Kayoko Ando, Takeshi Omasa, Junichiro Kotani

Cytotechnology　Vol. 65　No. 1　p. 15-22　2013/01　Research paper (scientific journal)

Inhibition of CYP3A4 by 6 ',7 '-dihydroxybergamottin in human CYP3A4 over-expressed hepG2 cells

Nobutaka Araki, Shuichi Tsuruoka, Gohki Hasegawa, Hayato Yanagihara, Takeshi Omasa, Shin Enosawa, Yasushi Yamazoe, Akio Fujimura

JOURNAL OF PHARMACY AND PHARMACOLOGY　Vol. 64　No. 12　p. 1715-1721　2012/12　Research paper (scientific journal)

Synthetic metabolic engineering-a novel, simple technology for designing a chimeric metabolic pathway

Xiaoting Ye, Kohsuke Honda, Takaaki Sakai, Kenji Okano, Takeshi Omasa, Ryuichi Hirota, Akio Kuroda, Hisao Ohtake

MICROBIAL CELL FACTORIES　Vol. 11　2012/09　Research paper (scientific journal)

Identification of the Replication Region of a 111-kb Circular Plasmid from Rhodococcus opacus B-4 by lambda Red Recombination-Based Deletion Analysis

Kohsuke Honda, Makoto Imura, Kenji Okano, Takeshi Omasa, Junichi Kato, Hisao Ohtake

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY　Vol. 76　No. 9　p. 1758-1764　2012/09　Research paper (scientific journal)

Action of Reactive Oxygen Species in the Antifungal Mechanism of Gemini-pyridinium Salts Against Yeast

Akihiro Shirai, Shouko Ueta, Hideaki Maseda, Hiroki Kourai, Takeshi Omasa

BIOCONTROL SCIENCE　Vol. 17　No. 2　p. 77-82　2012/06　Research paper (scientific journal)

Construction of BAC-based physical map and analysis of chromosome rearrangement in chinese hamster ovary cell lines

Yihua Cao, Shuichi Kimura, Takayuki Itoi, Kohsuke Honda, Hisao Ohtake, Takeshi Omasa

Biotechnology and Bioengineering　Vol. 109　No. 6　p. 1357-1367　2012/06　Research paper (scientific journal)

Construction of membrane-anchoring fusion protein of Thermococcus kodakaraensis glycerol kinase and its application to repetitive batchwise reactions

Elvi Restiawaty, Kohsuke Honda, Kenji Okano, Ryuichi Hirota, Takeshi Omasa, Akio Kuroda, Hisao Ohtake

Journal of Bioscience and Bioengineering　Vol. 113　No. 4　p. 521-525　2012/04　Research paper (scientific journal)

Enhancement of sialylation on humanized IgG-like bispecific antibody by overexpression of α2,6-sialyltransferase derived from Chinese hamster ovary cells

Masayoshi Onitsuka, Wook-Dong Kim, Hiroyuki Ozaki, Akira Kawaguchi, Kohsuke Honda, Hiroyuki Kajiura, Kazuhito Fujiyama, Ryutaro Asano, Izumi Kumagai, Hisao Ohtake, Takeshi Omasa

Applied Microbiology and Biotechnology　Vol. 94　No. 1　p. 69-80　2012/04　Research paper (scientific journal)

Fluorescence in situ hybridization using bacterial artificial chromosome (BAC) clones for the analysis of chromosome rearrangement in Chinese hamster ovary cells

Yihua Cao, Shuichi Kimura, Takayuki Itoi, Kohsuke Honda, Hisao Ohtake, Takeshi Omasa

Methods　Vol. 56　No. 3　p. 418-423　2012/03　Research paper (scientific journal)

N-terminal vacuolar sorting signal at the mouse antibody alters the N-linked glycosylation pattern in suspension-cultured tobacco BY2 cells

Ryo Misaki, Yohei Sakai, Takeshi Omasa, Kazuhito Fujiyama, Tatsuji Seki

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　Vol. 112　No. 5　p. 476-484　2011/11　Research paper (scientific journal)

Thermal analysis for differentiating between oleaginous and non-oleaginous microorganisms

Bongmun Kang, Kohsuke Honda, Kenji Okano, Tsunehiro Aki, Takeshi Omasa, Hisao Ohtake

BIOCHEMICAL ENGINEERING JOURNAL　Vol. 57　No. 15　p. 23-29　2011/11　Research paper (scientific journal)

Feasibility of thermophilic adenosine triphosphate-regeneration system using Thermus thermophilus polyphosphate kinase

Elvi Restiawaty, Yoshihiro Iwasa, Shohei Maya, Kohsuke Honda, Takeshi Omasa, Ryuichi Hirota, Akio Kuroda, Hisao Ohtake

Process Biochemistry　Vol. 46　No. 9　p. 1747-1752　2011/09　Research paper (scientific journal)

Hyaluronic acid production by recombinant Streptococcus thermophilus

Naoki Izawa, Masaki Serata, Toshiro Sone, Takeshi Omasa, Hisao Ohtake

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　Vol. 111　No. 6　p. 665-670　2011/06　Research paper (scientific journal)

Mutation in the sdeS gene promotes expression of the sdeAB efflux pump genes and multidrug resistance in Serratia marcescens

Hideaki Maseda, Yumiko Hashida, Akihiro Shirai, Takeshi Omasa, Taiji Nakae

Antimicrobial Agents and Chemotherapy　Vol. 55　No. 6　p. 2922-2926　2011/06　Research paper (scientific journal)

Simultaneous thermogravimetry and differential thermal analysis for comparing burning characteristics between oleaginous and non-oleaginous microorganisms

Bongmun Kang, Kohsuke Honda, Tsunehiro Aki, Takeshi Omasa, Hisao Ohtake

THERMOCHIMICA ACTA　Vol. 517　No. 1-2　p. 115-120　2011/04　Research paper (scientific journal)

Self-assembling peptide RADA16 as a scaffold in bone tissue engineering using dedifferentiated fat cells.

Kishimoto N, Momota Y, Hashimoto Y, Omasa T, Kotani J

Journal of Oral Tissue Engineering　Vol. 8　No. 3　p. 151-161　2011

ATF4 over-expression increased IgG1 productivity in Chinese hamster ovary cells.

Haredy AM, Nishizawa A, Honda K, Ohya T, Ohtake H, Omasa T

BMC proceedings　Vol. 5 Suppl 8　2011

Chromosome identification and its application in Chinese hamster ovary cells.

Cao Y, Kimura S, Park JY, Yamatani M, Honda K, Ohtake H, Omasa T

BMC proceedings　Vol. 5 Suppl 8　2011

Effects of palindrome structure on Dhfr amplification in Chinese hamster ovary cells

Joon Young Park, Miyuki Yamatani, Souhei Wadano, Yasuhiro Takagi, Kohsuke Honda, Takeshi Omasa, Hisao Ohtake

Process Biochemistry　Vol. 45　No. 12　p. 1845-1851　2010/12　Research paper (scientific journal)

Enhancement of recombinant enzyme activity in cpxA-deficient mutant of Escherichia coli

Ying Zhou, Takeshi Minami, Kohsuke Honda, Takeshi Omasa, Hisao Ohtake

Journal of Bioscience and Bioengineering　Vol. 110　No. 4　p. 403-407　2010/10　Research paper (scientific journal)

Production of 2-deoxyribose 5-phosphate from fructose to demonstrate a potential of artificial bio-synthetic pathway using thermophilic enzymes

Kohsuke Honda, Shohei Maya, Takeshi Omasa, Ryuichi Hirota, Akio Kuroda, Hisao Ohtake

Journal of Biotechnology　Vol. 148　No. 4　p. 204-207　2010/08　Research paper (scientific journal)

Systematic screening of Escherichia coli single-gene knockout mutants for improving recombinant whole-cell biocatalysts

Ying Zhou, Takeshi Minami, Kohsuke Honda, Takeshi Omasa, Hisao Ohtake

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY　Vol. 87　No. 2　p. 647-655　2010/06　Research paper (scientific journal)

Identification and analysis of specific chromosomal region adjacent to exogenous Dhfr-amplified region in Chinese hamster ovary cell genome

Joon Young Park, Yasuhiro Takagi, Miyuki Yamatani, Kohsuke Honda, Shuichi Asakawa, Nobuyoshi Shimizu, Takeshi Omasa, Hisao Ohtake

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　Vol. 109　No. 5　p. 504-511　2010/05　Research paper (scientific journal)

An Overview of the Current Mammalian Cell Technologies in Therapeutic Protein Production

OMASA Takeshi

KAGAKU TO SEIBUTSU　Vol. 48　No. 4　p. 255-262　2010/04/01

Cell Engineering and Cultivation of Chinese Hamster Ovary (CHO) Cells

Takeshi Omasa, Masayoshi Onitsuka, Wook-Dong Kim

CURRENT PHARMACEUTICAL BIOTECHNOLOGY　Vol. 11　No. 3　p. 233-240　2010/04

Electron Microscopic Analysis of Heat-Induced Leakage of Polyphosphate from a phoU Mutant of Escherichia coli

Hitoshi Kashihara, Bong Mun Kang, Takeshi Omasa, Kohsuke Honda, Yuka Sameshima, Akio Kuroda, Hisao Ohtake

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY　Vol. 74　No. 4　p. 865-868　2010/04　Research paper (scientific journal)

CHO遺伝子増幅細胞株における増幅領域の配列構造に基づいた遺伝子導入ベクターの構築と検討

山谷 美由希, 朴 俊映, 和田野 宗平, 高木 康弘, 本田 孝祐, 大竹 久夫, 大政 健史

化学工学会 研究発表講演要旨集　Vol. 2010　p. 961-961　2010

CHO BAC libraryを用いた染色体安定性評価法の開発

曹 溢華, 糸井 隆行, 上田 洋二, 近藤 哲司, 本田 孝祐, 大竹 久夫, 大政 健史

化学工学会 研究発表講演要旨集　Vol. 2010　p. 954-954　2010

Enhanced antibody production following intermediate addition based on flux analysis in mammalian cell continuous culture

Takeshi Omasa, Keisuke Furuichi, Tomoya Iemura, Yoshio Katakura, Michimasa Kishimoto, Ken-ichi Suga

BIOPROCESS AND BIOSYSTEMS ENGINEERING　Vol. 33　No. 1　p. 117-125　2010/01　Research paper (scientific journal)

Glycosylation pattern of humanized IgG-like bispecific antibody produced by recombinant CHO cells

Wook-Dong Kim, Miwako Tokunaga, Hiroyuki Ozaki, Takuya Ishibashi, Kohsuke Honda, Hiroyuki Kajiura, Kazuhito Fujiyama, Ryutaro Asano, Izumi Kumagai, Takeshi Omasa, Hisao Ohtake

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY　Vol. 85　No. 3　p. 535-542　2010/01　Research paper (scientific journal)

Bacterial Artificial Chromosome Library for Genome-Wide Analysis of Chinese Hamster Ovary Cells

Takeshi Omasa, Yihua Cao, Joon Young Park, Yasuhiro Takagi, Shuichi Kimura, Hidenori Yano, Kohsuke Honda, Shuichi Asakawa, Nobuyoshi Shimizu, Hisao Ohtake

BIOTECHNOLOGY AND BIOENGINEERING　Vol. 104　No. 5　p. 986-994　2009/12　Research paper (scientific journal)

Effect of cell-surface hydrophobicity on bacterial conversion of water-immiscible chemicals in two-liquid-phase culture systems

Takahiro Hamada, Yusuke Maeda, Hiroyuki Matsuda, Yuka Sameshima, Kohsuke Honda, Takeshi Omasa, Junichi Kato, Hisao Ohtake

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　Vol. 108　No. 2　p. 116-120　2009/08　Research paper (scientific journal)

Extractive lactic acid fermentation with tri-n-decylamine as the extractant

Min-Tian Gao, Takashi Shimamura, Nobuhiro Ishida, Eiji Nagamori, Haruo Takahashi, Shinji Umemoto, Takeshi Omasa, Hisao Ohtake

ENZYME AND MICROBIAL TECHNOLOGY　Vol. 44　No. 5　p. 350-354　2009/05　Research paper (scientific journal)

Change in glycosylation pattern with extension of endoplasmic reticulum retention signal sequence of mouse antibody produced by suspension-cultured tobacco BY2 cells

Kazuhito Fujiyama, Ryo Misaki, Yohei Sakai, Takeshi Omasa, Tatsuji Seki

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　Vol. 107　No. 2　p. 165-172　2009/02　Research paper (scientific journal)

Chinese hamster ovary cellにおけるBACを用いた染色体物理地図の構築

曹 溢華, 矢野 秀法, 木村 修一, 本田 孝祐, 大政 健史, 大竹 久夫

化学工学会 研究発表講演要旨集　Vol. 2009　p. 664-664　2009

遺伝子増幅CHO細胞における染色体上増幅領域の解明とその応用

朴 俊映, 高木 康弘, 山谷 美由希, 矢野 秀法, 本田 孝祐, 大政 健史, 大竹 久夫

化学工学会 研究発表講演要旨集　Vol. 2009　p. 630-630　2009

CHO遺伝子増幅細胞株における染色体上増幅領域を用いた発現システム構築

山谷 美由希, 朴 俊映, 高木 康弘, 矢野 秀法, 本田 孝祐, 大政 健史, 大竹 久夫

化学工学会 研究発表講演要旨集　Vol. 2009　p. 1045-1045　2009

Bone regeneration using dedifferentiated fat cells with PuraMatrixTM

Kishimoto N, Momota Y, Mori R, Hashimoto Y, Imai K, Omasa T, Kotani J

Journal of Oral Tissue Engineering　Vol. 6　No. 2　p. 127-134　2008/12　Research paper (scientific journal)

Overexpression of GADD34 Enhances Production of Recombinant Human Antithrombin III in Chinese Hamster Ovary Cells

Takeshi Omasa, Takashi Takami, Tomoshi Ohya, Eriko Kiyama, Tetsuji Hayashi, Hiroko Nishii, Hideo Miki, Kaoru Kobayashi, Kohsuke Honda, Hisao Ohtake

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　Vol. 106　No. 6　p. 568-573　2008/12　Research paper (scientific journal)

Human CYP3A4-introduced HepG2 cells: In vitro screening system of new chemicals for the evaluation of CYP3A4-inhibiting activity

N. Araki, S. Tsuruoka, N. Wang, G. Hasegawa, H. Yanagihara, H. Ando, T. Omasa, S. Enosawa, H. Nagai, A. Fujimura

Xenobiotica　Vol. 38　No. 11　p. 1355-1364　2008/11　Research paper (scientific journal)

Decrease in Antithrombin III Fucosylation by Expressing GDP-fucose Transporter siRNA in Chinese Hamster Ovary Cells (vol 106, pg 168, 2008)

Takeshi Omasa, Ryo Tanaka, Tomohiro Doi, Masayuki Ando, Yuka Kitamoto, Kobsuke Honda, Michimasa Kishimoto, Hisao Ohtake

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　Vol. 106　No. 4　p. 417-417　2008/10

A Comparison of Various Methods to Predict Bacterial Predilection for Organic Solvents Used as Reaction Media

Takahiro Hamada, Yuka Sameshima, Kohsuke Honda, Takeshi Omasa, Junichi Kato, Hisao Ohtake

Journal of Bioscience and Bioengineering　Vol. 106　No. 4　p. 357-362　2008/10　Research paper (scientific journal)

Decrease in antithrombin III fucosylation by expressing GDP-fucose transporter siRNA in Chinese hamster ovary cells

Takeshi Omasa, Ryo Tanaka, Tomohiro Doi, Masayuki Ando, Yuka Kitamoto, Kohsuke Honda, Michimasa Kishimoto, Hisao Ohtake

Journal of Bioscience and Bioengineering　Vol. 106　No. 2　p. 168-173　2008/08　Research paper (scientific journal)

Expression of Rhodococcus opacus alkB genes in anhydrous organic solvents

Yuka Sameshima, Kohsuke Honda, Junichi Kato, Takeshi Omasa, Hisao Ohtake

Journal of Bioscience and Bioengineering　Vol. 106　No. 2　p. 199-203　2008/08　Research paper (scientific journal)

Improved production of recombinant human antithrombin III in Chinese hamster ovary cells by ATF4 overexpression

Tomoshi Ohya, Tetsuji Hayashi, Eriko Kiyama, Hiroko Nishii, Hideo Miki, Kaoru Kobayashi, Kohsuke Honda, Takeshi Omasa, Hisao Ohtake

BIOTECHNOLOGY AND BIOENGINEERING　Vol. 100　No. 2　p. 317-324　2008/06　Research paper (scientific journal)

Stabilization of water-in-oil emulsion by Rhodococcus opacus B-4 and its application to biotransformation

Kohsuke Honda, Shiho Yamashita, Hiroyuki Nakagawa, Yuka Sameshima, Takeshi Omasa, Junichi Kato, Hisao Ohtake

Applied Microbiology and Biotechnology　Vol. 78　No. 5　p. 767-773　2008/04　Research paper (scientific journal)

タンパク質医薬品生産宿主としてのCHO細胞ゲノム解析とその応用

大政 健史, 大竹 久夫

化学工学会 研究発表講演要旨集　Vol. 2008　p. 416-416　2008

Heatphos法におけるケイ酸カルシウム水和物を用いたリン回収

中西 隆博, 上本 将英, 本田 孝祐, 大政 健史, 黒田 章夫, 美濃和 信孝, 大竹 久夫

化学工学会 研究発表講演要旨集　Vol. 2008　p. 972-972　2008

疎水性有機溶媒耐性細菌を用いた難水溶性物質変換反応におけるパラメーターの最適化

前田 裕介, 松田 裕之, 濱田 崇宏, 福間 真一, 本田 孝祐, 大政 健史, 加藤 純一, 大竹 久夫

化学工学会 研究発表講演要旨集　Vol. 2008　p. 973-973　2008

Production of mouse monoclonal antibody with galactose-extended sugar chain by suspension cultured tobacco BY2 cells expressing human beta(1,4)-galactosyltransferase

Kazuhito Fujiyama, Aiko Furukawa, Atsuya Katsura, Ryo Misaki, Takeshi Omasa, Tatsuji Seki

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　Vol. 358　No. 1　p. 85-91　2007/06　Research paper (scientific journal)

Utilization of hydrophobic bacterium Rhodococcus opacus B-4 as whole-cell catalyst in anhydrous organic solvents

Shiho Yamashita, Masafumi Satoi, Yoshihiro Iwasa, Kohsuke Honda, Yuka Sameshima, Takeshi Omasa, Junichi Kato, Hisao Ohtake

Applied Microbiology and Biotechnology　Vol. 74　No. 4　p. 761-767　2007/03　Research paper (scientific journal)

疎水性有機溶媒耐性細菌を用いた難水溶性物質の水酸化に関する基礎的検討

福間 真一, 前田 裕介, 山下 志保, 本田 孝祐, 大政 健史, 加藤 純一, 大竹 久夫

化学工学会 研究発表講演要旨集　Vol. 2007　p. 838-838　2007

Chinese Hamster Ovary (CHO) 細胞のゲノム解析と応用

大政 健史, 大竹 久夫

化学工学会 研究発表講演要旨集　Vol. 2007　p. 746-746　2007

遺伝子組換え酵母を用いた乳酸抽出発酵における阻害要因の検討

前田 裕介, 打味 奈緒子, 梅本 真司, 福間 真一, 本田 孝祐, 大政 健史, 石田 亘広, 長森 英二, 嶋村 隆, 高橋 治雄, 大竹 久夫

化学工学会 研究発表講演要旨集　Vol. 2007　p. 341-341　2007

小胞体ストレスを応用したCHO細胞における分泌能向上

木山 栄里子, 林 哲司, 大屋 智資, 三木 秀夫, 小林 薫, 本田 孝祐, 大政 健史, 大竹 久夫

化学工学会 研究発表講演要旨集　Vol. 2007　p. 419-419　2007

Integrated biooxidation and acid dehydration process for monohydroxylation of aromatics

Shiho Yamashita, Yuka Sameshima, Masaaki Konishi, Junichi Kato, Michimasa Kishimoto, Kohsuke Honda, Takeshi Omasa, Hisao Ohtake

Process Biochemistry　Vol. 42　No. 1　p. 46-51　2007/01　Research paper (scientific journal)

Recognition of culture state using two-dimensional gel electrophoresis with an artificial neural network

Naoki Izawa, Michimasa Kishimoto, Masaaki Konishi, Takeshi Omasa, Suteaki Shioya, Hisao Ohtake

Proteomics　Vol. 6　No. 13　p. 3730-3738　2006/07　Research paper (scientific journal)

N-linked glycan structures of a mouse monoclonal antibody produced from tobacco BY2 suspension-cultured cells

K Fujiyama, R Misaki, A Katsura, T Tanaka, A Furukawa, T Omasa, T Seki

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　Vol. 101　No. 3　p. 212-218　2006/03　Research paper (scientific journal)

Heatphos法によるリン回収における有機物巻き込み低減の検討

柏原 仁, 山本 悦司, 本田 孝祐, 大政 健史, 黒田 章夫, 大竹 久夫

化学工学会 研究発表講演要旨集　Vol. 2006　p. 926-926　2006

The significant improvement of survival times and pathological parameters by bioartificial liver with recombinant HepG2 in porcine liver failure model

Shin Enosawa, Tomoyuki Miyashita, Tomohiro Saito, Takeshi Omasa, Toshiharu Matsumura

CELL TRANSPLANTATION　Vol. 15　No. 10　p. 873-880　2006　Research paper (scientific journal)

Double-compartment cell culture apparatus: Construction and biochemical evaluation for bioartificial liver support

Masahiro Takahashi, Masayuki Sakurai, Shin Enosawa, Takeshi Omasa, Shuichi Tsuruoka, Toshiharu Matsumura

Cell Transplantation　Vol. 15　No. 10　p. 945-952　2006　Research paper (international conference proceedings)

The bioreactor with CYP3A4- and glutamine synthetase-introduced HepG2 Cells: Treatment of hepatic failure dog with diazepam overdosage

Ning Wang, Shuichi Tsuruoka, Hisashi Yamamoto, Shin Enosawa, Takeshi Omasa, Naohiro Sata, Toshiharu Matsumura, Hideo Nagai, Akio Fujimura

Artificial Organs　Vol. 29　No. 8　p. 681-684　2005/08　Research paper (scientific journal)

The separation of oil from an oil-water-bacteria mixture using a hydrophobic tubular membrane

M. Konishi, M. Kishimoto, N. Tamesui, T. Omasa, S. Shioya, H. Ohtake

Biochemical Engineering Journal　Vol. 24　No. 1　p. 49-54　2005/05/15　Research paper (scientific journal)

溶媒抽出による醗酵液からの乳酸回収法の検討

村田 雅洋, 梅本 真司, 大政 健史, 岸本 通雅, 大竹 久夫

化学工学会 研究発表講演要旨集　Vol. 2005　p. 301-301　2005

Toluene酸化微生物によるo-cresolの発酵生産

山下 志保, 小西 正朗, 里井 祐章, 中川 弘之, 大政 健史, 岸本 通雅, 大竹 久夫, 加藤 純一

化学工学会 研究発表講演要旨集　Vol. 2005　p. 241-241　2005

有機溶媒耐性細菌Rhodococcus opacusの培養と有用物質生産

里井 祐章, 鮫島 結香, 本田 孝祐, 大政 健史, 大竹 久夫, 恒川 博, 加藤 純一

化学工学会 研究発表講演要旨集　Vol. 2005　p. 279-279　2005

Biochemical engineering in cell and tissue engineering

T Omasa

SEIBUTSU-KOGAKU KAISHI　Vol. 83　No. 3　p. 117-122　2005　Research paper (scientific journal)

Construction and evaluation of drug-metabolizing cell line for bioartificial liver support system

T Omasa, K Kim, S Hiramatsu, Y Katakura, M Kishimoto, S Enosawa, H Ohtake

BIOTECHNOLOGY PROGRESS　Vol. 21　No. 1　p. 161-167　2005/01　Research paper (scientific journal)

Effect of sulfur sources on specific desulfurization activity of Rhodococcus erythropolis KA2-5-1 in exponential fed-batch culture

Masaaki Konishi, Michimasa Kishimoto, Takeshi Omasa, Yoshio Katakura, Suteaki Shioya, Hisao Ohtake

Journal of Bioscience and Bioengineering　Vol. 99　No. 3　p. 259-263　2005　Research paper (scientific journal)

Expression and amplification of glutamine synthetase gene endows HepG2 cells with ammonia-metabolizing activity for bioartificial liver support system

T Omasa, M Yamanaka, N Tanimura, Y Katakura, M Kishimoto, K Suga, S Enosawa

ENZYME AND MICROBIAL TECHNOLOGY　Vol. 35　No. 6-7　p. 519-524　2004/12　Research paper (scientific journal)

An attempt at decision making in tissue engineering: Reactor evaluation using the analytic hierarchy process (AHP)

Takeshi Omasa, Michimasa Kishimoto, Masaya Kawase, Kiyohito Yagi

Biochemical Engineering Journal　Vol. 20　No. 2-3　p. 173-179　2004/08/15　Research paper (scientific journal)

Rapid Construction of Gene-Amplified CHO Cell Line by Gene Targeting

Omasa Takeshi, Kajita Mihoko, Yoshikawa Tomohiro, Katakura Yoshio, Kishimoto Michimasa, Ohtake Hisao

Asian Pacific Confederation of Chemical Engineering congress program and abstracts　Vol. 2004　p. 488-488　2004

Changes in microflora duding composting of an aquatic plant, Brazilian elodea

T Nishida, T Fujimura, T Omasa, Y Katakura, K Suga, S Shioya

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN　Vol. 36　No. 10　p. 1201-1205　2003/10　Research paper (scientific journal)

Construction of a fuzzy control system for a bioreactor using biomass support particles

M Kishimoto, M Beluso, T Omasa, Y Katakura, H Fukuda, K Suga

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC　Vol. 17　No. 3-5　p. 207-213　2002/06　Research paper (scientific journal)

Analysis and optimization of biopanning process of phage display libraries

Y Katakura, GQ Zhuang, S Uchida, T Furuta, T Omasa, M Kishimoto, K Suga

BIOLOGICAL SYSTEMS ENGINEERING　Vol. 830　p. 271-284　2002　Research paper (scientific journal)

Knowledge/information sharing using mailing list: 6 years trial in biotechnology forum (btf)

T Omasa

NIPPON NOGEIKAGAKU KAISHI-JOURNAL OF THE JAPAN SOCIETY FOR BIOSCIENCE BIOTECHNOLOGY AND AGROCHEMISTRY　Vol. 76　No. 6　p. 531-533　2002　Research paper (scientific journal)

Expression and amplification of glutamine synthetase gene for constructing ammonia-metabolizing cell lines in hybrid bioartificial liver support system

T Omasa, K Kim, S Hiramatsu, Y Katakura, N Kishimoto, T Matsumura, S Enosawa, S Suzuki, H Amemiya, K Suga

ANIMAL CELL TECHNOLOGY : BASIC & APPLIED ASPECTS, VOL 12　p. 263-267　2002　Research paper (international conference proceedings)

Artificial mimicking of physiological active transport by a membrane co-cultured with two different cells: Hepatic origin HepG2 and renal origin PCTL-MDR

Mitsufumi Endo, Shin Enosawa, Michitaka Ozaki, Shuichi Tsuruoka, Shinya Hiramatsu, Kazumi Kim, Takeshi Omasa, Toshiharu Matsumura, Tatsuya Aoki, Yasuhisa Koyanagi, Seiichi Suzuki

Artificial Organs　Vol. 26　No. 9　p. 806-811　2002　Research paper (scientific journal)

Measurement of association rate constant of antibody-antigen interaction in solution based on enzyme-linked immunosorbent assay

GQ Zhuang, Y Katakura, T Omasa, M Kishimoto, KI Suga

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　Vol. 92　No. 4　p. 330-336　2001/10　Research paper (scientific journal)

A kinetic model for a biopanning process considering antigen desorption and effective antigen concentration on a solid phase

GQ Zhuang, Y Katakura, T Furuta, T Omasa, M Kishimoto, K Suga

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　Vol. 91　No. 5　p. 474-481　2001/05　Research paper (scientific journal)

Prolongation of survival of pigs with ischemic liver failure by treatment with a bioartificial liver using glutamine synthetase transfected recombinant HepG2

S. Enosawa, T. Miyashita, H. Tanaka, X. K. Li, S. Suzuki, H. Amemiya, T. Omasa, K. Suga, T. Matsumura

Transplantation Proceedings　Vol. 33　No. 1-2　p. 1945-1947　2001　Research paper (scientific journal)

A bioreactor with glutamine synthetase-transfected recombinant HepG2 cells exhibits ammonia removal activity without the need for added cofactors and substrates: Advantage of a cellular bioreactor over enzyme-immobilized beads

Shin Enosawa, Tomoyuki Miyashita, Xiao-Kaug Li, Seiichi Suzuki, Hiroshi Amemiya, Takeshi Omasa, Kenichi Suga, Toshiharu Matsumura

Journal of Artificial Organs　Vol. 4　No. 4　p. 348-352　2001　Research paper (scientific journal)

Application of circulatory flow bioreactor for long-term and large-scale culture of glutamine synthetase transduced CHO cells and its ammonia removal activity with an aim of development for a bioartificial liver assist system

S. Enosawa, T. Mukaiyama, T. Miyashita, X. K. Li, S. Suzuki, H. Amemiya, T. Matsumura, T. Omasa, K. Suga

Journal of Artificial Organs　Vol. 4　No. 1　p. 61-66　2001　Research paper (scientific journal)

In vivo estimation of bioartificial liver with recombinant HepG2 cells using pigs with ischemic liver failure

S. Enosawa, T. Miyashita, Y. Fujita, S. Suzuki, H. Amemiya, T. Omasa, S. Hiramatsu, K. Suga, T. Matsumura

Cell Transplantation　Vol. 10　No. 4-5　p. 429-433　2001　Research paper (international conference proceedings)

Coculture of hepatic and renal origin cell lines provides biohemofiltration with an active transport system of xenobiotics metabolites

"> Endo, Shin Enosawa, Seiichi Suzuki, Hiroshi Amemiya, Shuichi Tsuruoka, Akio Fujimura, Shinya Hiramatsu, Takeshi Omasa, Kenichi Suga, Toshiharu Matsumura, Tatsuya Aoki, Yasuhisa Koyanagi

Journal of Artificial Organs　Vol. 4　No. 4　p. 336-341　2001　Research paper (scientific journal)

L-lysine production by exponential feeding of L-threonine

K Tada, M Kishimoto, T Omasa, Y Katakura, KI Suga

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　Vol. 90　No. 6　p. 669-674　2000/12　Research paper (scientific journal)

Control of antibody-antigen interaction using anion-induced conformational change in antigen peptide

Y Katakura, T Miyazaki, H Wada, T Omasa, M Kishimoto, Y Goto, K Suga

PROTEIN ENGINEERING　Vol. 13　No. 10　p. 719-724　2000/10　Research paper (scientific journal)

Amplified gene location in chromosomal DNA affected recombinant protein production and stability of amplified genes

Y Yoshikawa, F Nakanishi, Y Ogura, D Oi, T Omasa, Y Katakura, M Kishimoto, K Suga

BIOTECHNOLOGY PROGRESS　Vol. 16　No. 5　p. 710-715　2000/09　Research paper (scientific journal)

Evaluation of stable and highly productive gene amplified CHO cell line based on the location of amplified genes

T Yoshikawa, F Nakanishi, S Itami, D Kameoka, T Omasa, Y Katakura, M Kishimoto, K Suga

CYTOTECHNOLOGY　Vol. 33　No. 1-3　p. 37-46　2000/07　Research paper (scientific journal)

Efficient production of desulfurizing cells with the aid of expert system

M Kishimoto, M Inui, T Omasa, Y Katakura, K Suga, K Okumura

BIOCHEMICAL ENGINEERING JOURNAL　Vol. 5　No. 2　p. 143-147　2000/06　Research paper (scientific journal)

On-line control of feeding of medium components to attain high cell density

H Suzuki, M Kishimoto, Y Kamoshita, T Omasa, Y Katakura, K Suga

BIOPROCESS ENGINEERING　Vol. 22　No. 5　p. 433-440　2000/05　Research paper (scientific journal)

Increase in desulfurization activity of Rhodococcus erythropolis KA2-5-1 using ethanol feeding

H Yan, M Kishimoto, T Omasa, Y Katakura, K Suga, K Okumura, O Yoshikawa

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　Vol. 89　No. 4　p. 361-366　2000/04　Research paper (scientific journal)

Development of a bioartificial liver with glutamine synthetase-transduced recombinant human hepatoblastoma cell line, HepG2

T. Miyashita, S. Enosawa, S. Suzuki, A. Tamura, H. Tanaka, H. Amemiya, T. Matsumura, T. Omasa, K. Suga, T. Aoki, Y. Koyanagi

Transplantation Proceedings　Vol. 32　No. 7　p. 2355-2358　2000　Research paper (scientific journal)

Long-term culture of glutamine synthetase-transfected HepG2 cells in circulatory flow bioreactor for development of a bioartificial liver

Shin Enosawa, Tomoyuki Miyashita, Seiichi Suzuki, Xiao-Kang Li, Miyuki Tsunoda, Hiroshi Amemiya, Mitsugu Yamanaka, Shinya Hiramatsu, Naoko Tanimura, Takeshi Omasa, Kenichi Suga, Toshiharu Matsumura

Cell Transplantation　Vol. 9　No. 5　p. 711-715　2000　Research paper (international conference proceedings)

Evaluation of stability in the DHFR gene amplification system using fluorescence in situ hybridization

F Nakanishi, T Yoshikawa, S Itami, T Omasa, Y Katakura, M Kishimoto, K Suga

ANIMAL CELL TECHNOLOGY: BASIC & APPLIED ASPECTS, VOL 10　p. 259-263　1999　Research paper (international conference proceedings)

Effect of sugar composition on the heterogeneity of antibody in hybridoma cultivation

T Omasa, Y Kitamoto, J Tanaka, Y Katakura, M Kishimoto, K Suga

ANIMAL CELL TECHNOLOGY: CHALLENGES FOR THE 21ST CENTURY　p. 139-143　1999　Research paper (international conference proceedings)

Energy metabolism and antibody production based on flux analysis in hybridoma

T Omasa, T Iemura, K Furuichi, Y Katakura, M Kishimoto, K Suga

ANIMAL CELL TECHNOLOGY: CHALLENGES FOR THE 21ST CENTURY　p. 11-15　1999　Research paper (international conference proceedings)

Construction of ammonia metabolizing cell line for artificial hybrid liver support system using recombinant DNA techniques

T Omasa, S Urayama, M Yamanaka, N Tanimura, Y Katakura, M Kishimoto, K Suga, S Enosawa, H Amemiya

KAGAKU KOGAKU RONBUNSHU　Vol. 24　No. 2　p. 184-189　1998/03　Research paper (scientific journal)

The importance of ionic strength as a parameter in screening peptide ligands from a phage display library

Y Katakura, ET Lim, S Tsujii, T Omasa, K Suga

JOURNAL OF FERMENTATION AND BIOENGINEERING　Vol. 85　No. 4　p. 447-450　1998　Research paper (scientific journal)

Selection and stability for a recombinant CHO cell line expressing human GM-CSF in gene amplification

T Omasa, S Itami, D Kameoka, Y Katakura, K Suga

ANIMAL CELL TECHNOLOGY: BASIC & APPLIED ASPECTS, VOL 9　p. 29-33　1998　Research paper (international conference proceedings)

Reduction of lake water eutrophication caused by nitrogen and phosphorous from paddy nearby field using a circular irrigation system

K Suga, N Fukui, M Tatani, Y Katakura, T Omasa, M Kishimoto

COMPUTER APPLICATIONS IN BIOTECHNOLOGY 1998　p. 553-559　1998　Research paper (international conference proceedings)

Effect of culture conditions on glycosylation in hybridoma antibody production

T Omasa, J Tanaka, K Amimoto, Y Katakura, K Suga

ANIMAL CELL TECHNOLOGY: BASIC & APPLIED ASPECTS, VOL 9　p. 35-39　1998　Research paper (international conference proceedings)

Effect of methanol concentration on the production of human beta(2)-glycoprotein I domain V by a recombinant Pichia pastoris: A simple system for the control of methanol concentration using a semiconductor Gas Sensor

Y Katakura, WH Zhang, GQ Zhuang, T Omasa, M Kishimoto, W Goto, KI Suga

JOURNAL OF FERMENTATION AND BIOENGINEERING　Vol. 86　No. 5　p. 482-487　1998　Research paper (scientific journal)

Energy metabolism and antibody production based on flux analysis in hybridoma cultivation

T Omasa, K Furuichi, T Iemura, Y Katakura, M Kishimoto, K Suga

COMPUTER APPLICATIONS IN BIOTECHNOLOGY 1998　p. 451-454　1998　Research paper (international conference proceedings)

An attempt to add biological functions by genetic engineering in order to produce high-performance bioreactor cells for hybrid artificial liver: Transfection of glutamine synthetase into Chinese hamster ovary (CHO) cell

S Enosawa, S Suzuki, M Fujino, H Amemiya, T Omasa, S Urayama, N Tanimura, K Suga

CELL TRANSPLANTATION　Vol. 6　No. 5　p. 537-540　1997/09　Research paper (scientific journal)

Fluorescence polarization study of a salt bridge between a single chain Fv and its antigen ribonuclease A

Y Katakura, T Kumamoto, Y Iwai, Y Kurokawa, T Omasa, KI Suga

MOLECULAR IMMUNOLOGY　Vol. 34　No. 12-13　p. 887-890　1997/08　Research paper (scientific journal)

Fluorescence polarization study of a salt bridge between a single-chain Fv and its antigen ribonuclease A

Y Katakura, T Kumamoto, Y Iwai, Y Kurokawa, T Omasa, K Suga

MOLECULAR IMMUNOLOGY　Vol. 34　No. 10　p. 731-734　1997/07　Research paper (scientific journal)

Analysis of antibody production based on energy metabolism in hybridoma

K Furuichi, T Iemura, T Omasa, Y Katakura, K Suga

ANIMAL CELL TECHNOLOGY: BASIC & APPLIED ASPECTS, VOL 8　p. 261-265　1997　Research paper (international conference proceedings)

Structure and function of the recombinant fifth domain of human β2-glycoprotein I: Effects of specific cleavage between Lys77 and Thr78

Yoshihisa Hagihara, Kei-Ichi Enjyoji, Takeshi Ômasa, Yoshio Katakura, Ken-Ichi Suga, Makoto Igarashi, Eiji Matsuura, Hisao Kato, Tetsuro Yoshimura, Yuji Goto

Journal of Biochemistry　Vol. 121　No. 1　p. 128-137　1997　Research paper (scientific journal)

Cloning of cDNA and characterization of anti-RNase A monoclonal antibody 3A21

Y Katakura, E Kobayashi, Y Kurokawa, T Omasa, K Fujiyama, KI Suga

JOURNAL OF FERMENTATION AND BIOENGINEERING　Vol. 82　No. 3　p. 312-314　1996　Research paper (scientific journal)

Enhancement effects of BSA and linoleic acid on hybridoma cell growth and antibody production

Masaki Kobayashi, Satoru Kato, Takeshi Omasa, Suteaki Shioya, Ken-ichi Suga

Cytotechnology　Vol. 15　No. 1-3　p. 51-56　1994/02　Research paper (scientific journal)

The enhancement of specific antibody production rate in glucose- and glutamine-controlled fed-batch culture

Takeshi Omasa, Masaru Ishimoto, Ken-ichi Higashiyama, Suteaki Shioya, Ken-ichi Suga

Cytotechnology　Vol. 8　No. 1　p. 75-84　1992/02　Research paper (scientific journal)