Recent advances in plant-based bioproduction

Kazuhito Fujiyama, Toshiya Muranaka, Atsushi Okazawa, Hikaru Seki, Goro Taguchi, Shuhei Yasumoto

Journal of Bioscience and Bioengineering　2024/04　Research paper (scientific journal)

Suppression of bolting in the Arabidopsis <i>hmg1</i> mutant under microgravity conditions in space – Possible involvement of lipid rafts

Takayuki Hoson, Kouichi Soga, Kazuyuki Wakabayashi, Rainer Hedrich, Masashi Suzuki, Toshiya Muranaka, Takashi Hashimoto, Hirofumi Hashimoto, Sachiko Yano, Shohei Matsumoto, Haruo Kasahara, Motoshi Kamada, Toru Shimazu

Biological Sciences in Space　Vol. 38　p. 18-26　2024　Research paper (scientific journal)

Glycyrrhizin Production in Licorice Hairy Roots Based on Metabolic Redirection of Triterpenoid Biosynthetic Pathway by Genome Editing.

Naoki Chiyo, Hikaru Seki, Takuya Kanamoto, Hiroshi Ueda, Mareshige Kojoma, Toshiya Muranaka

Plant & cell physiology　2023/12/28　Research paper (scientific journal)

Disruption of a licorice cellulose synthase-derived glycosyltransferase gene demonstrates its in planta role in soyasaponin biosynthesis

Manami Sakanishi, Soo Yeon Chung, Kentaro Fujiwara, Mareshige Kojoma, Toshiya Muranaka, Hikaru Seki

Plant Cell Reports　Vol. 43　No. 1　2023/12/23　Research paper (scientific journal)

A Newly Discovered Class of Triterpenoid Glycosyltransferase in Plants: Unexpected Functions Hidden in Cellulose Synthase-Like Proteins

Hikaru SEKI, Soo Yeon CHUNG, Toshiya MURANAKA

Vol. 61　No. 10　p. 477-483　2023/10/01　Research paper (scientific journal)

Integrated gene-free potato genome editing using transient transcription activator-like effector nucleases and regeneration-promoting gene expression by <i>Agrobacterium</i> infection

Naoyuki Umemoto, Shuhei Yasumoto, Muneo Yamazaki, Kenji Asano, Kotaro Akai, Hyoung Jae Lee, Ryota Akiyama, Masaharu Mizutani, Yozo Nagira, Kazuki Saito, Toshiya Muranaka

Plant Biotechnology　Vol. 40　No. 3　p. 211-218　2023/09/25　Research paper (scientific journal)

Foreign DNA detection in genome-edited potatoes by high-throughput sequencing

Shuhei Yasumoto, Toshiya Muranaka

Scientific Reports　Vol. 13　No. 1　2023/08/09　Research paper (scientific journal)

Class I and II NADPH-cytochrome P450 reductases exhibit different roles in triterpenoid biosynthesis in Lotus japonicus

Pramesti Istiandari, Shuhei Yasumoto, Hikaru Seki, Ery Odette Fukushima, Toshiya Muranaka

Frontiers in Plant Science　Vol. 14　2023/08/09　Research paper (scientific journal)

Chromosome-scale genome assembly of Glycyrrhiza uralensis revealed metabolic gene cluster centred specialized metabolites biosynthesis.

Amit Rai, Hideki Hirakawa, Megha Rai, Yohei Shimizu, Kenta Shirasawa, Shinji Kikuchi, Hikaru Seki, Mami Yamazaki, Atsushi Toyoda, Sachiko Isobe, Toshiya Muranaka, Kazuki Saito

DNA research : an international journal for rapid publication of reports on genes and genomes　Vol. 29　No. 6　2022/12/01　Research paper (scientific journal)

Identification of basic helix-loop-helix transcription factors that activate betulinic acid biosynthesis by RNA-sequencing of hydroponically culturedLotus japonicus

Hayato Suzuki, Hirokazu Takahashi, Ery Odette Fukushima, Mikio Nakazono, Toshiya Muranaka, Hikaru Seki

2022/11/17

Triterpenoids in aerenchymatous phellem contribute to internal root aeration and waterlogging adaptability in soybean

Hirokazu Takahashi, Chisato Abo, Hayato Suzuki, Jutapat Romsuk, Takao Oi, Asako Yanagawa, Tomoka Gorai, Yukari Tomisaki, Mana Jitsui, Satoshi Shimamura, Hitoshi Mori, Akito Kaga, Masao Ishimoto, Hikaru Seki, Toshiya Muranaka, Mikio Nakazono

2022/11/08

Identification of key amino acid residues toward improving the catalytic activity and substrate specificity of plant-derived cytochrome P450 monooxygenases CYP716A subfamily enzyme for triterpenoid production in Saccharomyces cerevisiae

Jutapat Romsuk, Shuhei Yasumoto, Hikaru Seki, Ery Odette Fukushima, Toshiya Muranaka

Frontiers in Bioengineering and Biotechnology　Vol. 10　2022/08/19　Research paper (scientific journal)

High-yield bioactive triterpenoid production by heterologous expression in Nicotiana benthamiana using the Tsukuba system

Jutapat Romsuk, Shuhei Yasumoto, Ery Odette Fukushima, Kenji Miura, Toshiya Muranaka, Hikaru Seki

Frontiers in Plant Science　Vol. 13　2022/08/18　Research paper (scientific journal)

Tandem Gene Duplication of Dioxygenases Drives the Structural Diversity of Steroidal Glycoalkaloids in the Tomato Clade.

Ryota Akiyama, Bunta Watanabe, Junpei Kato, Masaru Nakayasu, Hyoung Jae Lee, Naoyuki Umemoto, Toshiya Muranaka, Kazuki Saito, Yukihiro Sugimoto, Masaharu Mizutani

Plant & cell physiology　2022/05/13　Research paper (scientific journal)

Comparative Analysis of NADPH-Cytochrome P450 Reductases From Legumes for Heterologous Production of Triterpenoids in Transgenic Saccharomyces cerevisiae

Pramesti Istiandari, Shuhei Yasumoto, Pisanee Srisawat, Keita Tamura, Ayaka Chikugo, Hideyuki Suzuki, Hikaru Seki, Ery Odette Fukushima, Toshiya Muranaka

Frontiers in Plant Science　Vol. 12　2021/12/16　Research paper (scientific journal)

The biosynthetic pathway of potato solanidanes diverged from that of spirosolanes due to evolution of a dioxygenase

Ryota Akiyama, Bunta Watanabe, Masaru Nakayasu, Hyoung Jae Lee, Junpei Kato, Naoyuki Umemoto, Toshiya Muranaka, Kazuki Saito, Yukihiro Sugimoto, Masaharu Mizutani

Nature Communications　Vol. 12　No. 1　2021/12　Research paper (scientific journal)

Insights into the diversification of subclade IVa bHLH transcription factors in Fabaceae

Hayato Suzuki, Hikaru Seki, Toshiya Muranaka

BMC Plant Biology　Vol. 21　No. 1　2021/12　Research paper (scientific journal)

Characterization of C‐26 aminotransferase, indispensable for steroidal glycoalkaloid biosynthesis

Masaru Nakayasu, Naoyuki Umemoto, Ryota Akiyama, Kiyoshi Ohyama, Hyoung J. Lee, Haruka Miyachi, Bunta Watanabe, Toshiya Muranaka, Kazuki Saito, Yukihiro Sugimoto, Masaharu Mizutani

The Plant Journal　Vol. 108　No. 1　p. 81-92　2021/08/09　Research paper (scientific journal)

Characterization of UDP-glucose dehydrogenase isoforms in the medicinal legume Glycyrrhiza uralensis

Ayumi Kawasaki, Ayaka Chikugo, Keita Tamura, Hikaru Seki, Toshiya Muranaka

Plant Biotechnology　Vol. 38　No. 2　p. 205-218　2021/06/25　Research paper (scientific journal)

Tomato E8 Encodes a C-27 Hydroxylase in Metabolic Detoxification of α-Tomatine during Fruit Ripening.

Ryota Akiyama, Masaru Nakayasu, Naoyuki Umemoto, Junpei Kato, Midori Kobayashi, Hyoung Jae Lee, Yukihiro Sugimoto, Yoko Iijima, Kazuki Saito, Toshiya Muranaka, Masaharu Mizutani

Plant & cell physiology　Vol. 62　No. 5　p. 775-783　2021/06/08　Research paper (scientific journal)

Metabolic Engineering

Toshiya MURANAKA, Hikaru SEKI

CHEMISTRY　Vol. 76　No. 6　p. 66-67　2021/06

Allylic Hydroxylation Activity Is a Source of Saponin Chemodiversity in the Genus Glycyrrhiza

Much Z. Fanani, Satoru Sawai, Hikaru Seki, Masato Ishimori, Kiyoshi Ohyama, Ery O. Fukushima, Hiroshi Sudo, Kazuki Saito, Toshiya Muranaka

Plant and Cell Physiology　Vol. 62　No. 2　p. 262-271　2021/05/11　Research paper (scientific journal)

グリチルリチン生合成機構の解明と組換え酵母での生産

關 光, Soo Yeon Chung, 村中 俊哉

ファルマシア　Vol. 57　No. 8　p. 710-714　2021　Research paper (scientific journal)

酵母を用いた植物由来の甘味成分グリチルリチンの生産

Chung Soo Yeon, 關 光, 村中 俊哉

バイオサイエンスとインダストリー　Vol. 79　No. 3　p. 205-209　2021

Expression of two key enzymes of artemisinin biosynthesis FPS and ADS genes in saccharomyces cerevisiae

Elfahmi, Rizqiya Astri Hapsari, Tamara Chrysanthy, Vaniarta Synthiarini, Fifi Fitriyah Masduki, Agus Setiawan, Toshiya Muranaka

Advanced Pharmaceutical Bulletin　Vol. 11　No. 1　p. 181-187　2021/01/01　Research paper (scientific journal)

A cellulose synthase-derived enzyme catalyses 3-O-glucuronosylation in saponin biosynthesis

Soo Yeon Chung, Hikaru Seki, Yukiko Fujisawa, Yoshikazu Shimoda, Susumu Hiraga, Yuhta Nomura, Kazuki Saito, Masao Ishimoto, Toshiya Muranaka

Nature Communications　Vol. 11　No. 1　p. 5664-5664　2020/12　Research paper (scientific journal)

Production of the bioactive plant-derived triterpenoid morolic acid in engineered Saccharomyces cerevisiae

Pisanee Srisawat, Shuhei Yasumoto, Ery O. Fukushima, Jekson Robertlee, Hikaru Seki, Toshiya Muranaka

Biotechnology and Bioengineering　Vol. 117　No. 7　p. 2198-2208　2020/07/01　Research paper (scientific journal)

Preface to the special issue “technology in tissue culture toward horizon of plant biotechnology”

Yutaka Tabei, Toshiya Muranaka

Plant Biotechnology　Vol. 37　No. 2　p. 117-120　2020/06　Research paper (scientific journal)

Targeted genome editing in tetraploid potato through transient talen expression by agrobacterium infection

Shuhei Yasumoto, Satoru Sawai, Hyoung Jae Lee, Masaharu Mizutani, Kazuki Saito, Naoyuki Umemoto, Toshiya Muranaka

Plant Biotechnology　Vol. 37　No. 2　p. 205-211　2020/06　Research paper (scientific journal)

ロールプレイで一般市民から多様な意見を引き出す : 「ゲノム編集」をテーマに複数の視点で考え議論する

佐々 義子, 真山 武志, 田中 利一, 村中 俊哉, 小泉 望

遺伝 : 生物の科学　Vol. 74　No. 3　p. 314-320　2020/05

The effect of nojirimycin on the transcriptome of germinating Orobanche minor seeds

Atsushi Okazawa, Takatoshi Wakabayashi, Toshiya Muranaka, Yukihiro Sugimoto, Daisaku Ohta

Journal of Pesticide Science　Vol. 45　No. 4　p. 230-237　2020　Research paper (scientific journal)

Identification of α-tomatine 23-hydroxylase involved in the detoxification of a bitter glycoalkaloid

Masaru Nakayasu, Ryota Akiyama, Midori Kobayashi, Hyoung Jae Lee, Takashi Kawasaki, Bunta Watanabe, Shingo Urakawa, Junpei Kato, Yukihiro Sugimoto, Yoko Iijima, Kazuki Saito, Toshiya Muranaka, Naoyuki Umemoto, Masaharu Mizutani

Plant and Cell Physiology　Vol. 61　No. 1　p. 21-28　2020/01/01　Research paper (scientific journal)

Structure-Activity Relationships of Pentacyclic Triterpenoids as Inhibitors of Cyclooxygenase and Lipoxygenase Enzymes

Nhu Ngoc Quynh Vo, Yuhta Nomura, Toshiya Muranaka, Ery Odette Fukushima

Journal of Natural Products　Vol. 82　No. 12　p. 3311-3320　2019/12/27　Research paper (scientific journal)

Characterization of steroid 5α-reductase involved in α-tomatine biosynthesis in tomatoes.

Ryota Akiyama, Hyoung Jae Lee, Masaru Nakayasu, Keishi Osakabe, Yuriko Osakabe, Naoyuki Umemoto, Kazuki Saito, Toshiya Muranaka, Yukihiro Sugimoto, Masaharu Mizutani

Plant biotechnology (Tokyo, Japan)　Vol. 36　No. 4　p. 253-263　2019/12/25　Research paper (scientific journal)

Isolation of Artemisia capillaris membrane-bound di-prenyltransferase for phenylpropanoids and redesign of artepillin C in yeast

Ryosuke Munakata, Tomoya Takemura, Kanade Tatsumi, Eiko Moriyoshi, Koki Yanagihara, Akifumi Sugiyama, Hideyuki Suzuki, Hikaru Seki, Toshiya Muranaka, Noriaki Kawano, Kayo Yoshimatsu, Nobuo Kawahara, Takao Yamaura, Jérémy Grosjean, Frédéric Bourgaud, Alain Hehn, Kazufumi Yazaki

Communications Biology　Vol. 2　No. 1　2019/12/01　Research paper (scientific journal)

Molecular Basis of C-30 Product Regioselectivity of Legume Oxidases Involved in High-Value Triterpenoid Biosynthesis

Much Zaenal Fanani, Ery Odette Fukushima, Satoru Sawai, Jianwei Tang, Masato Ishimori, Hiroshi Sudo, Kiyoshi Ohyama, Hikaru Seki, Kazuki Saito, Toshiya Muranaka

Frontiers in Plant Science　Vol. 10　No. 1520　p. 1520-1520　2019/11/26　Research paper (scientific journal)

Lotus japonicus Triterpenoid Profile and Characterization of the CYP716A51 and LjCYP93E1 Genes Involved in Their Biosynthesis in Planta

Hayato Suzuki, Ery Odette Fukushima, Yuko Shimizu, Hikaru Seki, Yukiko Fujisawa, Masao Ishimoto, Keishi Osakabe, Yuriko Osakabe, Toshiya Muranaka

Plant and Cell Physiology　Vol. 60　No. 11　p. 2496-2509　2019/11/01　Research paper (scientific journal)

Toward elucidating the regulatory mechanisms of glycyrrhizin biosynthesis

Vol. 3　No. 11　p. 1038-1041　2019/10

Isolation of Artemisia capillaris membrane-bound di-prenyltransferase for phenylpropanoids and redesign of artepillin C in yeast

Ryosuke Munakata, Tomoya Takemura, Kanade Tatsumi, Eiko Moriyoshi, Koki Yanagihara, Akifumi Sugiyama, Hideyuki Suzuki, Hikaru Seki, Toshiya Muranaka, Noriaki Kawano, Kayo Yoshimatsu, Nobuo Kawahara, Takao Yamaura, Jeremy Grosjean, Frederic Bourgaud, Alain Hehn, Kazufumi Yazaki

COMMUNICATIONS BIOLOGY　Vol. 2　p. 384-384　2019/10　Research paper (scientific journal)

Efficient genome engineering using Platinum TALEN in potato

Shuhei Yasumoto, Naoyuki Umemotoz, Hyoung Jae Lee, Masaru Nakayasu, Satoru Sawai, Tetsushi Sakuma, Takashi Yamamoto, Masaharu Mizutani, Kazuki Saito, Toshiya Muranaka

PLANT BIOTECHNOLOGY　Vol. 36　No. 3　p. 167-173　2019/09　Research paper (scientific journal)

Functional specialization of UDP-glycosyltransferase 73P12 in licorice to produce a sweet triterpenoid saponin, glycyrrhizin

Yuhta Nomura, Hikaru Seki, Tomonori Suzuki, Kiyoshi Ohyama, Masaharu Mizutani, Tomomi Kaku, Keita Tamura, Eiichiro Ono, Manabu Horikawa, Hiroshi Sudo, Hiroaki Hayashi, Kazuki Saito, Toshiya Muranaka

Plant Journal　Vol. 99　No. 6　p. 1127-1143　2019/09/01　Research paper (scientific journal)

Identification and characterization of (+)-α-bisabolol and 7-epi-silphiperfol-5-ene synthases from Artemisia abrotanum

Paskorn Muangphrom, Momoka Misaki, Munenori Suzuki, Masaya Shimomura, Hideyuki Suzuki, Hikaru Seki, Toshiya Muranaka

Phytochemistry　Vol. 164　p. 144-153　2019/08　Research paper (scientific journal)

Atrazine exposed phytoplankton causes the production of non-viable offspring on Daphnia magna

Pijar Religia, Yasuhiko Kato, Ery Odette Fukushima, Tomoaki Matsuura, Toshiya Muranaka, Hajime Watanabe

Marine Environmental Research　Vol. 145　p. 177-183　2019/03　Research paper (scientific journal)

Genome editing of tetraploid potato towards societal implementation

Vol. 7　No. 2　p. 42-46　2019/02

Identification of a 3b-hydroxysteroid dehydrogenase/ 3-ketosteroid reductase involved in a-tomatine biosynthesis in tomato

Hyoung Jae Lee, Masaru Nakayasu, Ryota Akiyama, Midori Kobayashi, Haruka Miyachi, Yukihiro Sugimoto, Naoyuki Umemoto, Kazuki Saito, Toshiya Muranaka, Masaharu Mizutani

Plant and Cell Physiology　Vol. 60　No. 6　p. 1304-1315　2019　Research paper (scientific journal)

Identification of oxidosqualene cyclases from the medicinal legume tree Bauhinia forficata: a step toward discovering preponderant α-amyrin-producing activity

Pisanee Srisawat, Ery Odette Fukushima, Shuhei Yasumoto, Jekson Robertlee, Hideyuki Suzuki, Hikaru Seki, Toshiya Muranaka

New Phytologist　Vol. 224　No. 1　p. 352-366　2019　Research paper (scientific journal)

The mevalonate pathway but not the methylerythritol phosphate pathway is critical for elaioplast and pollen coat development in arabidopsis

Keiko Kobayashi, Masashi Suzuki, Toshiya Muranaka, Noriko Nagata

Plant Biotechnology　Vol. 35　No. 4　p. 381-385　2018/12/25

Transcriptome sequencing and identification of cytochrome p450 monooxygenases involved in the biosynthesis of maslinic acid and corosolic acid in Avicennia marina

Mitsuki Nakamura, Tran My Linh, Le Quynh Lien, Hayato Suzuki, Nguyen Chi Mai, Vu Huong Giang, Keita Tamura, Nguyen van Thanh, Hideyuki Suzuki, Ryo Misaki, Toshiya Muranaka, Ninh Khac Ban, Kazuhito Fujiyama, Hikaru Seki

Plant Biotechnology　Vol. 35　No. 4　p. 341-348　2018/12/25　Research paper (scientific journal)

Establishment of a modified CRISPR/Cas9 system with increased mutagenesis frequency using the translational enhancer dMac3 and multiple guide RNAs in potato

Hiroaki Kusano, Mariko Ohnuma, Hiromi Mutsuro-Aoki, Takahiro Asahi, Dai Ichinosawa, Hitomi Onodera, Kenji Asano, Takahiro Noda, Takaaki Horie, Kou Fukumoto, Miho Kihira, Hiroshi Teramura, Kazufumi Yazaki, Naoyuki Umemoto, Toshiya Muranaka, Hiroaki Shimada

Scientific Reports　Vol. 8　No. 1　p. 13753-13753　2018/12/01　Research paper (scientific journal)

Genetic and functional characterization of Sg-4 glycosyltransferase involved in the formation of sugar chain structure at the C-3 position of soybean saponins

Kyoko Takagi, Ryoichi Yano, Saeko Tochigi, Yukiko Fujisawa, Hiroki Tsuchinaga, Yuya Takahashi, Yoshitake Takada, Akito Kaga, Toyoaki Anai, Chigen Tsukamoto, Hikaru Seki, Toshiya Muranaka, Masao Ishimoto

Phytochemistry　Vol. 156　p. 96-105　2018/12　Research paper (scientific journal)

ゲノム編集による農作物の品種改良 : イネ,トマト,ジャガイモからさらに拡大 (特集 ゲノム編集)

安本 周平, 藤本 大輝, 村中 俊哉

遺伝 : 生物の科学　Vol. 72　No. 6　p. 585-590　2018/11

Generation of α-solanine-free hairy roots of potato by CRISPR/Cas9 mediated genome editing of the St16DOX gene

Masaru Nakayasu, Ryota Akiyama, Hyoung Jae Lee, Keishi Osakabe, Yuriko Osakabe, Bunta Watanabe, Yukihiro Sugimoto, Naoyuki Umemoto, Kazuki Saito, Toshiya Muranaka, Masaharu Mizutani

Plant Physiology and Biochemistry　Vol. 131　p. 70-77　2018/10　Research paper (scientific journal)

Medicago truncatulaのβ-アミリンC30-オキシダーゼの産物の部位選択性決定において鍵となるアミノ酸残基の同定(Identification of key residue determining product regioselectivity of Medicago truncatula beta-amyrin C30-oxidase)

Fanani Much Zaenal, Fukushima Ery Odette, Sawai Satoru, Ohyama Kiyoshi, Seki Hikaru, Saito Kazuki, Muranaka Toshiya

日本生物工学会大会講演要旨集　Vol. 平成30年度　p. 199-199　2018/08

Generation of -solanine-free hairy roots of potato by CRISPR/Cas9 mediated genome editing of the St16DOX gene

Nakayasu Masaru, Akiyama Ryota, Lee Jae Hyoung, Keishi Osakabe, Yuriko Osakabe, Watanabe Bunta, Sugimoto Yukihiro, Umemoto Naoyuki, Saito Kazuki, Muranaka Toshiya, Mizutani Masaharu

Plant Physiology and Biochemistry : PPB　Vol. Vol.131　p. 70-77　2018/04　Research paper (scientific journal)

Isolation and Characterization of the Soybean Sg-3 Gene that is Involved in Genetic Variation in Sugar Chain Composition at the C-3 Position in Soyasaponins

Ryoichi Yano, Kyoko Takagi, Saeko Tochigi, Yukiko Fujisawa, Yuhta Nomura, Hiroki Tsuchinaga, Yuya Takahashi, Yoshitake Takada, Akito Kaga, Toyoaki Anai, Chigen Tsukamoto, Hikaru Seki, Toshiya Muranaka, Masao Ishimoto

PLANT AND CELL PHYSIOLOGY　Vol. 59　No. 4　p. 797-810　2018/04　Research paper (scientific journal)

The Basic Helix-Loop-Helix Transcription Factor GubHLH3 Positively Regulates Soyasaponin Biosynthetic Genes in Glycyrrhiza uralensis

Keita Tamura, Koki Yoshida, Yasuko Hiraoka, Daiki Sakaguchi, Ayaka Chikugo, Keiichi Mochida, Mareshige Kojoma, Nobutaka Mitsuda, Kazuki Saito, Toshiya Muranaka, Hikaru Seki

PLANT AND CELL PHYSIOLOGY　Vol. 59　No. 4　p. 783-796　2018/04　Research paper (scientific journal)

四倍体作物，ジャガイモのゲノム編集:ジャガイモ育種の革新

梅基 直行, 水谷 正治, 村中 俊哉

化学と生物　Vol. 56　No. 8　p. 566-572　2018

What is the most suited host to produce plant useful metabolites? : Towards production of plant specialized metabolites by highly-developed plant tissue culture system

Vol. 70　No. 3　p. 40-45　2018

Evidence that the arabidopsis thaliana 3-hydroxy-3-methylglutaryl-coa reductase 1 is phosphorylated at ser577 in planta

Jekson Robertlee, Keiko Kobayashi, Jianwei Tang, Masashi Suzuki, Toshiya Muranaka

Plant Biotechnology　Vol. 35　No. 1　p. 1-7　2018　Research paper (scientific journal)

Isolation and Characterization of the Soybean Sg-3 Gene that is Involved in Genetic Variation in Sugar Chain Composition at the C-3 Position in Soyasaponins

Yano, R., Takagi, K., Tochigi, S., Fujisawa, Y., Nomura, Y., Tsuchinaga, H., Takahashi, Y., Takada, Y., Kaga, A., Anai, T., Tsukamoto, C., Seki, H., Muranaka, T., Ishimoto, M.

Plant and Cell Physiology　Vol. 59　No. 4　p. 792-805　2018　Research paper (scientific journal)

Identification and characterization of a novel sesquiterpene synthase, 4-amorphen-11-ol synthase, from artemisia maritima

Paskorn Muangphrom, Hikaru Seki, Seiya Matsumoto, Mika Nishiwaki, Ery O. Fukushima, Toshiya Muranaka

Plant Biotechnology　Vol. 35　No. 2　p. 113-121　2018　Research paper (scientific journal)

Comparative analysis of CYP716A subfamily enzymes for the heterologous production of C-28 oxidized triterpenoids in transgenic yeast

Hayato Suzuki, Ery Odette Fukushima, Naoyuki Umemoto, Kiyoshi Ohyama, Hikaru Seki, Toshiya Muranaka

Plant Biotechnology　Vol. 35　No. 2　p. 131-139　2018　Research paper (scientific journal)

Plant-derived isoprenoid sweeteners: Recent progress in biosynthetic gene discovery and perspectives on microbial production

Hikaru Seki, Keita Tamura, Toshiya Muranaka

Bioscience, Biotechnology and Biochemistry　Vol. 82　No. 6　p. 927-934　2018　Research paper (scientific journal)

Dark conditions enhance aluminum tolerance in several rice cultivars via multiple modulations of membrane sterols

Tadao Wagatsuma, Eriko Maejima, Toshihiro Watanabe, Tomonobu Toyomasu, Masaharu Kuroda, Toshiya Muranaka, Kiyoshi Ohyama, Akifumi Ishikawa, Masami Usui, Shahadat Hossain Khan, Hayato Maruyama, Keitaro Tawaraya, Yuriko Kobayashi, Hiroyuki Koyama

JOURNAL OF EXPERIMENTAL BOTANY　Vol. 69　No. 3　p. 567-577　2018/01　Research paper (scientific journal)

Revealing the catalytic residues of amorpha-4,11-diene synthase (ADS): new insight for engineering terpene synthases

Muangphrom P, Seki H, Muranaka T

Biotarget　Vol. 1　No. 19　2017/12　Research paper (scientific journal)

A dioxygenase catalyzes steroid 16α-hydroxylation in steroidal glycoalkaloid biosynthesis

Masaru Nakayasu, Naoyuki Umemoto, Kiyoshi Ohyama, Yoshinori Fujimoto, Hyoung Jae Lee, Bunta Watanabe, Toshiya Muranaka, Kazuki Saito, Yukihiro Sugimoto, Masaharu Mizutani

Plant Physiology　Vol. 175　No. 1　p. 120-133　2017/09　Research paper (scientific journal)

Cytochrome P450 Monooxygenase CYP716A141 is a Unique b-Amyrin C-16b Oxidase Involved in Triterpenoid Saponin Biosynthesis in Platycodon grandiflorus (vol 57, pg 874, 2017)

Keita Tamura, Yuga Teranishi, Shinya Ueda, Hideyuki Suzuki, Noriaki Kawano, Kayo Yoshimatsu, Kazuki Saito, Nobuo Kawahara, Toshiya Muranaka, Hikaru Seki

PLANT AND CELL PHYSIOLOGY　Vol. 58　No. 6　p. 1119-1119　2017/06　Research paper (scientific journal)

Cytochrome P450 monooxygenase CYP716A141 is a unique β-amyrin C-16β oxidase involved in triterpenoid saponin biosynthesis in Platycodon grandiflorus

Keita Tamura, Yuga Teranishi, Shinya Ueda, Hideyuki Suzuki, Noriaki Kawano, Kayo Yoshimatsu, Kazuki Saito, Nobuo Kawahara, Toshiya Muranaka, Hikaru Seki

Plant and Cell Physiology　Vol. 58　No. 5　p. 874-884　2017/05/01　Research paper (scientific journal)

AKIN10, a representative Arabidopsis SNF1-related protein kinase 1 (SnRK1), phosphorylates and downregulates plant HMG-CoA reductase

Jekson Robertlee, Keiko Kobayashi, Masashi Suzuki, Toshiya Muranaka

FEBS Letters　Vol. 591　No. 8　p. 1159-1166　2017/04　Research paper (scientific journal)

Current status and future of genome editing technologies for breeding of agricultural products

Ezura Hiroshi, Ohsawa Ryo, Hirose Sakiko, Komatsu Akira, Ando Ikuo, Kondo Motohiko, Ariizumi Tohru, Miura Kenji, Muranaka Toshiya, Gen Koichiro

Breeding Research　Vol. 19　No. 1　p. 14-20　2017/03

CYP716A179 functions as a triterpene C-28 oxidase in tissue-cultured stolons of Glycyrrhiza uralensis

Keita Tamura, Hikaru Seki, Hideyuki Suzuki, Mareshige Kojoma, Kazuki Saito, Toshiya Muranaka

Plant Cell Reports　Vol. 36　No. 3　p. 437-445　2017/03/01　Research paper (scientific journal)

Functional characterization of CYP716 family P450 enzymes in triterpenoid biosynthesis in tomato

Shuhei Yasumoto, Hikaru Seki, Yuko Shimizu, Ery O. Fukushima, Toshiya Muranaka

Frontiers in Plant Science　Vol. 8　No. JANUARY　p. 21-21　2017/01/30　Research paper (scientific journal)

Molecular breeding of SGA-free potatoes accumulating pharmaceutically useful saponins

Akiyama Ryota, Nakayasu Masaru, Umemoto Naoyuki, Muranaka Toshiya, Mizutani Masaharu

Regulation of Plant Growth & Development　Vol. 52　No. 2　p. 92-98　2017

Genome editing for polyploid- and vegetative crop, potato

Vol. 1　No. 1　p. 23-26　2017/01

Structure and hemolytic activity relationships of triterpenoid saponins and sapogenins

Nhu Ngoc Quynh Vo, Ery Odette Fukushima, Toshiya Muranaka

Journal of Natural Medicines　Vol. 71　No. 1　p. 50-58　2017/01/01　Research paper (scientific journal)

Platform for “chemical metabolic switching” to increase sesquiterpene content in plants

Keiko Kobayashi, Kanako Kobayashi, Haruhiko Yamaguchi, Yukino Miyagi Inoue, Keiji Takagi, Kazuhisa Fushihara, Hikaru Seki, Masashi Suzuki, Noriko Nagata, Toshiya Muranaka

Plant Biotechnology　Vol. 34　No. 1　p. 65-69　2017

Draft genome assembly and annotation of Glycyrrhiza uralensis, a medicinal legume

Keiichi Mochida, Tetsuya Sakurai, Hikaru Seki, Takuhiro Yoshida, Kotaro Takahagi, Satoru Sawai, Hiroshi Uchiyama, Toshiya Muranaka, Kazuki Saito

Plant Journal　Vol. 89　No. 2　p. 181-194　2017/01/01　Research paper (scientific journal)

Glycyrrhizin production in hairy root cultures of Glycyrrhiza uralensis induced triterpenoid biosynthetic gene

M. Kojoma, H. Seki, K. Ohyama, S. Y. Kim, T. Muranaka

PLANTA MEDICA　Vol. 82　2016/12

Two Cytochrome P450 Monooxygenases Catalyze Early Hydroxylation Steps in the Potato Steroid Glycoalkaloid Biosynthetic Pathway

Naoyuki Umemoto, Masaru Nakayasu, Kiyoshi Ohyama, Mari Yotsu-Yamashita, Masaharu Mizutani, Hikaru Seki, Kazuki Saito, Toshiya Muranaka

PLANT PHYSIOLOGY　Vol. 171　No. 4　p. 2458-2467　2016/08　Research paper (scientific journal)

Functional analysis of amorpha-4,11-diene synthase (ADS) homologs from non-artemisinin-producing artemisia species: The discovery of novel koidzumiol and (+)-a-Bisabolol synthases

Paskorn Muangphrom, Hikaru Seki, Munenori Suzuki, Aya Komori, Mika Nishiwaki, Ryota Mikawa, Ery Odette Fukushima, Toshiya Muranaka

Plant and Cell Physiology　Vol. 57　No. 8　p. 1678-1688　2016/08/01　Research paper (scientific journal)

毒にも薬にもなる植物の話 : パン酵母で薬を造る、毒のないジャガイモを創る (特集 生物毒)

村中 俊哉

Milsil : 自然と科学の情報誌　Vol. 9　No. 4　p. 11-13　2016/07　Research paper (scientific journal)

Functional characterization of CYP71D443, a cytochrome P450 catalyzing C-22 hydroxylation in the 20-hydroxyecdysone biosynthesis of Ajuga hairy roots

Yuki Tsukagoshi, Kiyoshi Ohyama, Hikaru Seki, Tomoyoshi Akashi, Toshiya Muranaka, Hideyuki Suzuki, Yoshinori Fujimoto

PHYTOCHEMISTRY　Vol. 127　p. 23-28　2016/07　Research paper (scientific journal)

Artemisinin-based antimalarial research: application of biotechnology to the production of artemisinin, its mode of action, and the mechanism of resistance of Plasmodium parasites

Paskorn Muangphrom, Hikaru Seki, Ery Odette Fukushima, Toshiya Muranaka

Journal of Natural Medicines　Vol. 70　No. 3　p. 318-334　2016/07/01

Ajuga 24-Sterol Reductase Catalyzes the Direct Reductive Conversion of 24-Methylenecholesterol to Campesterol

Yuki Tsukagoshi, Hideyuki Suzuki, Hikaru Seki, Toshiya Muranaka, Kiyoshi Ohyama, Yoshinori Fujimoto

JOURNAL OF BIOLOGICAL CHEMISTRY　Vol. 291　No. 15　p. 8189-8198　2016/04　Research paper (scientific journal)

Novel triterpene oxidizing activity of Arabidopsis thaliana CYP716A subfamily enzymes

Shuhei Yasumoto, Ery O. Fukushima, Hikaru Seki, Toshiya Muranaka

FEBS LETTERS　Vol. 590　No. 4　p. 533-540　2016/02　Research paper (scientific journal)

Biosynthesis of Plant-Derived Natural Zero-Calorie Sweeteners

Vol. 221　No. 3　p. 225-231　2016

Identification of furostanol glycoside 26-O-β-glucosidase involved in steroidal saponin biosynthesis from Dioscorea esculenta

Masaru Nakayasu, Takashi Kawasaki, Hyoung Jae Lee, Yukihiro Sugimoto, Michio Onjo, Toshiya Muranaka, Masaharu Mizutani

Plant Biotechnology　Vol. 32　No. 4　p. 299-308　2015/12/29　Research paper (scientific journal)

Identification and genome organization of saponin pathway genes from a wild crucifer, and their use for transient production of saponins in Nicotiana benthamiana

Bekzod Khakimov, Vera Kuzina, Pernille Erthmann, Ery Odette Fukushima, Jörg M. Augustin, Carl Erik Olsen, Jelle Scholtalbers, Hanne Volpin, Sven Bode Andersen, Thure P. Hauser, Toshiya Muranaka, Søren Bak

Plant Journal　Vol. 84　No. 3　p. 478-490　2015/11　Research paper (scientific journal)

P450s and UGTs: Key Players in the Structural Diversity of Triterpenoid Saponins

Hikaru Seki, Keita Tamura, Toshiya Muranaka

Plant and Cell Physiology　Vol. 56　No. 8　p. 1463-1471　2015/08/01　Research paper (scientific journal)

Planteose as a storage carbohydrate required for early stage of germination of Orobanche minor and its metabolism as a possible target for selective control

Takatoshi Wakabayashi, Benesh Joseph, Shuhei Yasumoto, Tomoyoshi Akashi, Toshio Aoki, Kazuo Harada, Satoru Muranaka, Takeshi Bamba, Eiichiro Fukusaki, Yasutomo Takeuchi, Koichi Yoneyama, Toshiya Muranaka, Yukihiro Sugimoto, Atsushi Okazawa

JOURNAL OF EXPERIMENTAL BOTANY　Vol. 66　No. 11　p. 3085-3097　2015/06　Research paper (scientific journal)

TALENを用いたゲノム編集による植物代謝改変

安本周平, 關 光, 村中俊哉

生物工学会誌　Vol. 92　No. 10　p. 553-555　2014/10

Successful expression of a novel bacterial gene for pinoresinol reductase and its effect on lignan biosynthesis in transgenic Arabidopsis thaliana

Masayuki Tamura, Yukiko Tsuji, Tatsuya Kusunose, Atsushi Okazawa, Naofumi Kamimura, Tetsuya Mori, Ryo Nakabayashi, Shojiro Hishiyama, Yuki Fukuhara, Hirofumi Hara, Kanna Sato-Izawa, Toshiya Muranaka, Kazuki Saito, Yoshihiro Katayama, Masao Fukuda, Eiji Masai, Shinya Kajita

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY　Vol. 98　No. 19　p. 8165-8177　2014/10　Research paper (scientific journal)

Sterol side chain reductase 2 is a key enzyme in the biosynthesis of cholesterol, the common precursor of toxic steroidal glycoalkaloids in potato

Satoru Sawai, Kiyoshi Ohyama, Shuhei Yasumoto, Hikaru Seki, Tetsushi Sakuma, Takashi Yamamoto, Yumiko Takebayashi, Mikiko Kojima, Hitoshi Sakakibara, Toshio Aoki, Toshiya Muranaka, Kazuki Saito, Naoyuki Umemoto

Plant Cell　Vol. 26　No. 9　p. 3763-3774　2014/09/01　Research paper (scientific journal)

Study on the effect of nojirimycin on sugar metabolism in germinating seeds for selective control of root parasitic weeds

Takatoshi Wakabayashi, Shuhei Yasumoto, Tomoyoshi Akashi, Toshio Aoki, Yukihiro Sugimoto, Daisaku Ohta, Toshiya Muranaka, Atsushi Okazawa

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY　Vol. 248　2014/08

特集によせて(<特集>植物バイオテクノロジー実用化研究の最前線)

岡澤 敦司, 村中 俊哉

生物工学会誌 : seibutsu-kogaku kaishi　Vol. 92　No. 10　p. 536-536　2014

Plant cytochrome P450s in triterpenoid biosynthesis: Diversity and application to combinatorial biosynthesis

Ery Odette Fukushima, Hikaru Seki, Toshiya Muranaka

Fifty Years of Cytochrome P450 Research　p. 125-134　2014/01/01　Part of collection (book)

Heterologous expression of triterpene biosynthetic genes in yeast and Subsequent Metabolite Identification Through GC-MS

Ery Odette Fukushima, Hikaru Seki, Toshiya Muranaka

Methods in Molecular Biology　Vol. 1153　p. 235-243　2014　Research paper (scientific journal)

The role of Arabidopsis ABCG9 and ABCG31 ATP binding cassette transporters in pollen fitness and the deposition of steryl glycosides on the pollen coat

Hyunju Choi, Kiyoshi Ohyama, Yu Young Kim, Jun Young Jin, Saet Buyl Lee, Yasuyo Yamaoka, Toshiya Muranaka, Mi Chung Suh, Shozo Fujioka, Youngsook Lee

Plant Cell　Vol. 26　No. 1　p. 310-324　2014/01　Research paper (scientific journal)

Functional analysis of orthologous artemisinic aldehyde Δ11(13)-reductase reveals potential artemisinin-producing activity in non-artemisinin-producing Artemisia absinthium

Paskorn Muangphrom, Munenori Suzuki, Hikaru Seki, Ery Odette Fukushima, Toshiya Muranaka

Plant Biotechnology　Vol. 31　No. 5　p. 483-491　2014　Research paper (scientific journal)

Glucosyltransferase activity of Arabidopsis UGT71C1 towards pinoresinol and lariciresinol

Atsushi Okazawa, Tatsuya Kusunose, Eiichiro Ono, Hyun Jung Kim, Honoo Satake, Bun Ichi Shimizu, Masaharu Mizutani, Hikaru Seki, Toshiya Muranaka

Plant Biotechnology　Vol. 31　No. 5　p. 561-566　2014　Research paper (scientific journal)

植物テルペノイド代謝の多様性とコンビナトリアル生合成

福島エリオデット, 關 光, 村中俊哉

生物工学会誌　Vol. 91　No. 6　p. 337-341　2013/06

Combinatorial biosynthesis of legume natural and rare triterpenoids in engineered yeast

Ery O. Fukushima, Hikaru Seki, Satoru Sawai, Munenori Suzuki, Kiyoshi Ohyama, Kazuki Saito, Toshiya Muranaka

Plant and Cell Physiology　Vol. 54　No. 5　p. 740-749　2013/05　Research paper (scientific journal)

Glycyrrhiza uralensis transcriptome landscape and study of phytochemicals

Jordan A. Ramilowski, Satoru Sawai, Hikaru Seki, Keiichi Mochida, Takuhiro Yoshida, Tetsuya Sakurai, Toshiya Muranaka, Kazuki Saito, Carsten O. Daub

Plant and Cell Physiology　Vol. 54　No. 5　p. 697-710　2013/05　Research paper (scientific journal)

Phytochemical genomics on the way

Toshiya Muranaka, Kazuki Saito

Plant and Cell Physiology　Vol. 54　No. 5　p. 645-646　2013/05

Multi-Gene Transformation for Pathway Engineering of Secondary Metabolites

Hideyuki Suzuki, Eiji Takita, Kiyoshi Ohyama, Satoru Sawai, Hikaru Seki, Nozomu Sakurai, Toshiya Muranaka, Masao Ishimoto, Hiroshi Sudo, Kazuki Saito, Daisuke Shibata

The Handbook of Plant Metabolomics　p. 227-244　2013/04　Part of collection (book)

Comparative functional analysis of CYP71AV1 natural variants reveals an important residue for the successive oxidation of amorpha-4,11-diene

Aya Komori, Munenori Suzuki, Hikaru Seki, Tomoko Nishizawa, Jacobus Johannes Marion Meyer, Hideaki Shimizu, Shigeyuki Yokoyama, Toshiya Muranaka

FEBS Letters　Vol. 587　No. 3　p. 278-284　2013/01/31　Research paper (scientific journal)

Functional analysis of HMG-CoA reductase and oxidosqualene cyclases in arabidopsis

Toshiya Muranaka, Kiyoshi Ohyama

Isoprenoid Synthesis in Plants and Microorganisms: New Concepts and Experimental Approaches　p. 465-474　2013/01/01　Part of collection (book)

Development of Capsicum EST-SSR markers for species identification and in silico mapping onto the tomato genome sequence

Kenta Shirasawa, Kohei Ishii, Cholgwang Kim, Tomohiro Ban, Munenori Suzuki, Takashi Ito, Toshiya Muranaka, Megumi Kobayashi, Noriko Nagata, Sachiko Isobe, Satoshi Tabata

Molecular Breeding　Vol. 31　No. 1　p. 101-110　2013　Research paper (scientific journal)

Differences in plant growth and leaf sesamin content of the lignan-rich sesame variety 'Gomazou' under continuous light of different wavelengths

Naoki Hata, Yoshinori Hayashi, Eiichiro Ono, Honoo Satake, Akio Kobayashi, Toshiya Muranaka, Atsushi Okazawa

Plant Biotechnology　Vol. 30　No. 1　p. 1-8　2013　Research paper (scientific journal)

Identification of marneral synthase, which is critical for growth and development in Arabidopsis

Young S. Go, Saet B. Lee, Hae J. Kim, Jungmook Kim, Hyo Young Park, Jeong Kook Kim, Kyomi Shibata, Takao Yokota, Kiyoshi Ohyama, Toshiya Muranaka, Siméon Arseniyadis, Mi C. Suh

Plant Journal　Vol. 72　No. 5　p. 791-804　2012/12　Research paper (scientific journal)

Green leaf volatiles enhance methyl jasmonate response in Arabidopsis

Toshihiro Hirao, Atsushi Okazawa, Kazuo Harada, Akio Kobayashi, Toshiya Muranaka, Kazumasa Hirata

Journal of Bioscience and Bioengineering　Vol. 114　No. 5　p. 540-545　2012/11　Research paper (scientific journal)

植物遺伝資源確保に向けた有用遺伝子単離とその利用

村中 俊哉, 小森 彩

生物工学会誌 : seibutsu-kogaku kaishi　Vol. 90　No. 10　p. 623-624　2012/10/25

Photobiocatalyzed asymmetric reduction of ketones using Chlorella sp. MK201

Ken ichi Itoh, Kaoru Nakamura, Tadashi Aoyama, Ryusuke Matsuba, Tsuyoshi Kakimoto, Masahiko Murakami, Rio Yamanaka, Toshiya Muranaka, Hiroshi Sakamaki, Toshio Takido

Biotechnology Letters　Vol. 34　No. 11　p. 2083-2086　2012/10　Research paper (scientific journal)

植物の代謝多様性とその応用 : この10年と今後の展開

村中 俊哉

生物工学会誌 : seibutsu-kogaku kaishi　Vol. 90　No. 9　p. 572-575　2012/09/25

Towards the production of plant thriterpenoids of economical value using engineered yeasts

SEKI Hikaru, MURANAKA Toshiya

Vol. 70　No. 3　p. 192-195　2012/05

β-amyrin oxidation by oat CYP51H10 expressed heterologously in yeast cells: The first example of CYP51-dependent metabolism other than the 14-demethylation of sterol precursors

Mieko Kunii, Yutaka Kitahama, Ery Odette Fukushima, Hikaru Seki, Toshiya Muranaka, Yuzo Yoshida, Yuri Aoyama

Biological and Pharmaceutical Bulletin　Vol. 35　No. 5　p. 801-804　2012/05　Research paper (scientific journal)

Phosphoproteome exploration reveals a reformatting of cellular processes in response to low sterol biosynthetic capacity in Arabidopsis

Dimitri Heintz, Sebastien Gallien, Vincent Compagnon, Anne Berna, Masashi Suzuki, Shigeo Yoshida, Toshiya Muranaka, Alain Van Dorsselaer, Christine Schaeffer, Thomas J. Bach, Hubert Schaller

Journal of Proteome Research　Vol. 11　No. 2　p. 1228-1239　2012/02/03　Research paper (scientific journal)

Metabolism of plant diversity and its application - This decade and future developments-

Village, T.

Seibutsu-kogaku Kaishi　Vol. 90　No. 9　2012　Research paper (scientific journal)

β-Amyrin oxidation by oat CYP51H10 expressed heterologously in yeast cells: the first example of CYP51-dependent metabolism other than the 14-demethylation of sterol precursors.

Mieko Kunii, Yutaka Kitahama, Ery Odette Fukushima, Hikaru Seki, Toshiya Muranaka, Yuzo Yoshida, Yuri Aoyama

Biological & pharmaceutical bulletin　Vol. 35　No. 5　p. 801-4　2012　Research paper (scientific journal)

Application of Continuous Light in a Plant Factory System : 2. Growth Habit and Occurrence of Injury in Asteraceae and Other Crops Grown Under Continuous Light

HATA Naoki, MASUDA Masaharu, KOBAYASHI Akio, MURANAKA Toshiya, OKAZAWA Atsushi, MURAKAMI Kenji

Shokubutsu Kankyo Kogaku　Vol. 23　No. 4　p. 127-136　2011/12/01

Application of Continuous Light in a Plant Factory System : 3. Moderation of Injuries Induced by Continuous Light and Relative Tolerance to Continuous Light

HATA Naoki, MASUDA Masaharu, KOBAYASHI Akio, MURANAKA Toshiya, OKAZAWA Atsushi, MURAKAMI Kenji

Shokubutsu Kankyo Kogaku　Vol. 23　No. 4　p. 137-143　2011/12/01

Production of Triterpenoids by Recombinant Yeast

Bio industry　Vol. 28　No. 12　p. 6-12　2011/12

組換え酵母を用いた植物テルペノイドの生産

關 光, 村中俊哉

生物工学会誌　Vol. 89　No. 11　p. 656-659　2011/12

CYP716A subfamily members are multifunctional oxidases in triterpenoid biosynthesis

Ery O. Fukushima, Hikaru Seki, Kiyoshi Ohyama, Eiichiro Ono, Naoyuki Umemoto, Masaharu Mizutani, Kazuki Saito, Toshiya Muranaka

Plant and Cell Physiology　Vol. 52　No. 12　p. 2050-2061　2011/12　Research paper (scientific journal)

A New Insight into Application for Barley Chromosome Addition Lines of Common Wheat: Achievement of Stigmasterol Accumulation

Jianwei Tang, Kiyoshi Ohyama, Kanako Kawaura, Hiromi Hashinokuchi, Yoko Kamiya, Masashi Suzuki, Toshiya Muranaka, Yasunari Ogihara

PLANT PHYSIOLOGY　Vol. 157　No. 3　p. 1555-1567　2011/11　Research paper (scientific journal)

Triterpene functional genomics in licorice for identification of CYP72A154 involved in the biosynthesis of glycyrrhizin

Hikaru Seki, Satoru Sawai, Kiyoshi Ohyama, Masaharu Mizutani, Toshiyuki Ohnishi, Hiroshi Sudo, Ery Odette Fukushima, Tomoyoshi Akashi, Toshio Aoki, Kazuki Saito, Toshiya Muranaka

Plant Cell　Vol. 23　No. 11　p. 4112-4123　2011/11　Research paper (scientific journal)

Application of Continuous Light in a Plant Factory System : 1. Growth Habit and Occurrence of Injury in Solanaceae and Cucurbitaceae Crops Grown Under Continuous Light

HATA Naoki, MASUDA Masaharu, KOBAYASHI Akio, MURANAKA Toshiya, OKAZAWA Atsushi, MURAKAMI Kenji

Shokubutsu Kankyo Kogaku　Vol. 23　No. 3　p. 93-100　2011/09/01

特集によせて(<特集>植物遺伝子の魅力～生物工学分野への応用)

村中 俊哉, 中山 亨

生物工学会誌 : seibutsu-kogaku kaishi　Vol. 89　No. 11　p. 642-642　2011

Metabolic pathways and engineering in plants

Vol. 63　No. 4　p. 81-83,図巻頭1p　2011

Plant gateway vectors for RNAi as a tool for functional genomic studies.

Toshiya Muranaka

Methods in molecular biology (Clifton, N.J.)　Vol. 744　p. 27-35　2011　Research paper (scientific journal)

植物遺伝子の魅力 : 生物工学分野への応用

村中 俊哉, 田口 悟朗, 中山 亨

生物工学会誌 : seibutsu-kogaku kaishi　Vol. 88　No. 12　p. 672-672　2010/12/25

Agrobacterium-Mediated Transformation of Euphorbia tirucalli Callus

Hidenobu Uchida, Hirofumi Yamashita, Toyoaki Anai, Toshiya Muranaka, Kanji Ohyama

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY　Vol. 74　No. 4　p. 851-853　2010/04　Research paper (scientific journal)

Production of pharmaceuticals by plant tissue cultures

Toshiya Muranaka, Kazuki Saito

Comprehensive Natural Products II: Chemistry and Biology　Vol. 3　p. 615-628　2010/03　Part of collection (book)

The mitochondrial PPR protein LOVASTATIN INSENSITIVE 1 plays regulatory roles in cytosolic and plastidial isoprenoid biosynthesis through RNA editing

Jianwei Tang, Keiko Kobayashi, Masashi Suzuki, Shogo Matsumoto, Toshiya Muranaka

PLANT JOURNAL　Vol. 61　No. 3　p. 456-466　2010/02　Research paper (scientific journal)

The study of metabolic regulation of sesquiterpenoids in plants

Vol. 59　No. 1　p. 59-68　2010　Research paper (bulletin of university, research institution)

The AMI1 gene family: Indole-3-acetamide hydrolase functions in auxin biosynthesis in plants

Yoshihiro Mano, Keiichirou Nemoto, Masashi Suzuki, Hikaru Seki, Isao Fujii, Toshiya Muranaka

Journal of Experimental Botany　Vol. 61　No. 1　p. 25-32　2010/01

Upregulation of phytosterol and triterpene biosynthesis in Centella asiatica hairy roots overexpressed ginseng farnesyl diphosphate synthase

Ok Tae Kim, Sun Hee Kim, Kiyoshi Ohyama, Toshiya Muranaka, Yong Eui Choi, Hyeon Yong Lee, Min Young Kim, Baik Hwang

Plant Cell Reports　Vol. 29　No. 4　p. 403-411　2010　Research paper (scientific journal)

In vitro proliferation and triterpenoid characteristics of licorice (glycyrrhiza uralensis fischer, leguminosae) stolons

Mareshige Kojoma, Kiyoshi Ohyama, Hikaru Seki, Yasuko Hiraoka, Sandra Naomi Asazu, Satoko Sawa, Haruo Sekizaki, Shigeo Yoshida, Toshiya Muranaka

Plant Biotechnology　Vol. 27　No. 1　p. 59-66　2010　Research paper (scientific journal)

Triterpenoid levels are reduced during Euphorbia tirucalli L. callus formation

Hidenobu Uchida, Kiyoshi Ohyama, Masashi Suzuki, Hirofumi Yamashita, Toshiya Muranaka, Kanji Ohyama

PLANT BIOTECHNOLOGY　Vol. 27　No. 1　p. 105-109　2010　Research paper (scientific journal)

The molecular cloning of dihydroartemisinic aldehyde reductase and its implication in artemisinin biosynthesis in artemisia annua

Anna Margareta Rydén, Carolien Ruyter-Spira, Wim J. Quax, Hiroyuki Osada, Toshiya Muranaka, Oliver Kayser, Harro Bouwmeester

Planta Medica　Vol. 76　No. 15　p. 1778-1783　2010　Research paper (scientific journal)

Function of the aux and rol genes of the Ri plasmid in plant cell division in vitro

Keiichirou Nemoto, Masamitsu Hara, Masashi Suzuki, Hikaru Seki, Atsuhiro Oka, Toshiya Muranaka, Yoshihiro Mano

Plant Signaling and Behavior　Vol. 4　No. 12　p. 1145-1147　2009/12　Research paper (scientific journal)

Discovery of new steroidal biosynthetic pathway in plants

OHYAMA Kiyoshi, SUZUKI Masashi, MURANAKA Toshiya

Bioscience & industry　Vol. 67　No. 8　p. 428-430　2009/11/01

Isolation and Identification of a Novel Chlorophenol from a Cell Suspension Culture of Helichrysum aureonitens

Seyed Mahdi Ziaratnia, Kiyoshi Ohyama, Ahmed Abdel-Fattah Hussein, Toshiya Muranaka, Namrita Lall, Karl Josef Kunert, Jacobus Johannes Marion Meyer

CHEMICAL & PHARMACEUTICAL BULLETIN　Vol. 57　No. 11　p. 1282-1283　2009/11　Research paper (scientific journal)

カンゾウのグリチルリチン生合成遺伝子ディスカバリーと今後の研究展開

村中 俊哉, 關 光, 大山 清

ファルマシア　Vol. 45　No. 9　p. 865-869　2009/09/01

薬用植物カンゾウ(甘草、Glycyrrhiza uralensis)のストロン培養システムによるクローン増殖について

高上馬 希重, 浅津 なをみサンドラ, 澤 聡子, 関崎 春雄, 關 光, 平岡 靖子, 村中 俊哉, 大山 清, 林 茂樹, 柴田 敏郎

根の研究 = Root research　Vol. 18　No. 2　p. 64-64　2009/06/26

Complete blockage of the mevalonate pathway results in male gametophyte lethality

Masashi Suzuki, Shoko Nakagawa, Yukiko Kamide, Keiko Kobayashi, Kiyoshi Ohyama, Hiromi Hashinokuchi, Reiko Kiuchi, Kazuki Saito, Toshiya Muranaka, Noriko Nagata

Journal of Experimental Botany　Vol. 60　No. 7　p. 2055-2064　2009/05　Research paper (scientific journal)

Cloning and characterization of a squalene synthase gene from a petroleum plant, Euphorbia tirucalli L.

Hidenobu Uchida, Hirofumi Yamashita, Masataka Kajikawa, Kiyoshi Ohyama, Osamu Nakayachi, Ryuji Sugiyama, Katsuyuki T. Yamato, Toshiya Muranaka, Hideya Fukuzawa, Miho Takemura, Kanji Ohyama

PLANTA　Vol. 229　No. 6　p. 1243-1252　2009/05　Research paper (scientific journal)

The aux1 gene of the Ri plasmid is sufficient to confer auxin autotrophy in tobacco BY-2 cells

Keiichirou Nemoto, Masamitsu Hara, Shingo Goto, Kouji Kasai, Hikaru Seki, Masashi Suzuki, Atsuhiro Oka, Toshiya Muranaka, Yoshihiro Mano

Journal of Plant Physiology　Vol. 166　No. 7　p. 729-738　2009/05/01　Research paper (scientific journal)

薬用植物甘草のグリチルリチン生合成遺伝子の発見 -組み換え酵母で生合成中間体の生産に成功-

關 光, 大山 清, 村中俊哉

化学と生物　Vol. 47　No. 2　p. 714-716　2009/02

The NtAMI1 gene functions in cell division of tobacco BY-2 cells in the presence of indole-3-acetamide

Keiichirou Nemoto, Masamitsu Hara, Masashi Suzuki, Hikaru Seki, Toshiya Muranaka, Yoshihiro Mano

FEBS Letters　Vol. 583　No. 2　p. 487-492　2009/01/22　Research paper (scientific journal)

Dual biosynthetic pathways to phytosterol via cycloartenol and lanosterol in Arabidopsis

Kiyoshi Ohyama, Masashi Suzuki, Jun Kikuchi, Kazuki Saito, Toshiya Muranaka

Proceedings of the National Academy of Sciences of the United States of America　Vol. 106　No. 3　p. 725-730　2009/01/20　Research paper (scientific journal)

Metabolic diversity of triterpenes in plants

Ohyama Kiyoshi, Muranaka Toshiya

Regulation of Plant Growth & Development　Vol. 44　No. 1　p. 56-66　2009

Interorganellar Network on the Regulatory Mechanisms of Higher Plants Specific IPP Biosynthesis

Kobayashi Keiko, Suzuki Masashi, Muranaka Toshiya

PLANT MORPHOLOGY　Vol. 21　No. 1　p. 47-53　2009

Expressed sequence tags from rhizomes of Glycyrrhiza uralensis

Hiroshi Sudo, Hikaru Seki, Nozomu Sakurai, Hideyuki Suzuki, Daisuke Shibata, Atsushi Toyoda, Yasushi Totoki, Yoshiyuki Sakaki, Osamu Lida, Toshiro Shibata, Mareshige Kojoma, Toshiya Muranaka, Kazuki Saito

Plant Biotechnology　Vol. 26　No. 1　p. 105-107　2009　Research paper (scientific journal)

The NtAMI1 gene functions in cell division of tobacco BY-2 cells in the presence of indole-3-acetamide

Keiichirou Nemoto, Masamitsu Hara, Masashi Suzuki, Hikaru Seki, Toshiya Muranaka, Yoshihiro Mano

FEBS LETTERS　Vol. 583　No. 2　p. 487-492　2009/01　Research paper (scientific journal)

Albinism and cell viability in cycloartenol synthase deficient Arabidopsis

Elena Babiychuk, Pierrette Bouvier-Navé, Vincent Compagnon, Masashi Suzuki, Toshiya Muranaka, Marc Van Montagu, Sergei Kushnir, Hubert Schaller

Plant Signaling and Behavior　Vol. 3　No. 11　p. 978-980　2008/11　Research paper (scientific journal)

Licorice β-amyrin 11-oxidase, a cytochrome P450 with a key role in the biosynthesis of the triterpene sweetener glycyrrhizin

Hikaru Seki, Kiyoshi Ohyama, Satoru Sawai, Masaharu Mizutani, Toshiyuki Ohnishi, Hiroshi Sudo, Tomoyoshi Akashi, Toshio Aoki, Kazuki Saito, Toshiya Muranaka

Proceedings of the National Academy of Sciences of the United States of America　Vol. 105　No. 37　p. 14204-14209　2008/09/16　Research paper (scientific journal)

The mechanisms of steroidal backbone synthesis in plant

SUZUKI Masashi, OHYAMA Kiyoshi, MURANAKA Toshiya

Bioscience & industry　Vol. 66　No. 9　p. 517-519　2008/09/01

Dolichol biosynthesis and its effects on the unfolded protein response and abiotic stress resistance in Arabidopsis

Hairong Zhang, Kiyoshi Ohyama, Julie Boudet, Zhizhong Chen, Jilai Yang, Min Zhang, Toshiya Muranaka, Christophe Maurel, Jian-Kang Zhu, Zhizhong Gong

PLANT CELL　Vol. 20　No. 7　p. 1879-1898　2008/07　Research paper (scientific journal)

Enhanced secretory activity of Atropa belladonna hairy root culture over-expressing ADP-ribosylation factor gene

Yuki Asakura, Hikaru Seki, Toshiya Muranaka, Yoshimi Yamamura, Fumiya Kurosaki

Biological and Pharmaceutical Bulletin　Vol. 31　No. 7　p. 1465-1468　2008/07　Research paper (scientific journal)

The roles of sterols on formation of lipid-rich organelles in tapetum and pollen coat: ultrastructural analysis of mutants exhibiting deficiency of sterol methyltransferase

Suzuki Eriko, Suzuki Masashi, Ohyama Kiyoshi, Muranaka Toshiya, Nagata Noriko

Journal of Japan Women's University. Faculty of science　No. 16　p. 29-33　2008/03　Research paper (bulletin of university, research institution)

Allelic mutant series reveal distinct functions for Arabidopsis cycloartenol synthase 1 in cell viability and plastid biogenesis

Elena Babiychuk, Pierrette Bouvier-Nave, Vincent Compagnon, Masashi Suzuki, Toshiya Muranaka, Marc Van Montagu, Sergei Kushnir, Hubert Schaller

Proceedings of the National Academy of Sciences of the United States of America　Vol. 105　No. 8　p. 3163-3168　2008/02/26　Research paper (scientific journal)

The resist wall experiment on EMCS

T. Hoson, S. Matsumoto, K. Soga, K. Wakabayashi, T. Hashimoto, S. Sonobe, T. Muranaka, S. Kamisaka, M. Kamada, K. Omori, N. Ishioka, T. Shimazu

European Space Agency, (Special Publication) ESA SP　Vol. 663 SP　2008　Research paper (international conference proceedings)

The "all-in-one" rol-type binary vectors as a tool for functional genomic studies using hairy roots

Hikaru Seki, Kiyoshi Ohyama, Tomoko Nishizawa, Shigeo Yoshida, Toshiya Muranaka

Plant Biotechnology　Vol. 25　No. 4　p. 347-355　2008　Research paper (scientific journal)

Novel root culture system using a recessive mutant with a rooty phenotype

Masashi Suzuki, Keiko Kobayashi, Noriko Nagata, Osamu Matsuda, Koh Iba, Shigeo Yoshida, Toshiya Muranaka

Plant Biotechnology　Vol. 25　No. 2　p. 197-200　2008　Research paper (scientific journal)

Chemical phenotypes of the hmg1 and hmg2 mutants of Arabidopsis demonstrate the in-planta role of HMG-CoA reductase in triterpene biosynthesis

Kiyoshi Ohyama, Masashi Suzuki, Kazuo Masuda, Shigeo Yoshida, Toshiya Muranaka

Chemical and Pharmaceutical Bulletin　Vol. 55　No. 10　p. 1518-1521　2007/10　Research paper (scientific journal)

Plants utilize isoprene emission as a thermotolerance mechanism

Kanako Sasaki, Takuya Saito, Mari Lämsä, Kirsi Marja Oksman-Caldentey, Masashi Suzuki, Kiyoshi Ohyama, Toshiya Muranaka, Kazuaki Ohara, Kazufumi Yazaki

Plant and Cell Physiology　Vol. 48　No. 9　p. 1254-1262　2007/09　Research paper (scientific journal)

Lovastatin insensitive 1, a novel pentatricopeptide repeat protein, is a potential regulatory factor of isoprenoid biosynthesis in Arabidopsis

Keiko Kobayashi, Masashi Suzuki, Jianwei Tang, Noriko Nagata, Kiyoshi Ohyama, Hikaru Seki, Reiko Kiuchi, Yasuko Kaneko, Miki Nakazawa, Minami Matsui, Shogo Matsumoto, Shigeo Yoshida, Toshiya Muranaka

Plant and Cell Physiology　Vol. 48　No. 2　p. 322-331　2007/02　Research paper (scientific journal)

Characterization and engineering of glycosyltransferases responsible for steroid saponin biosynthesis in Solanaceous plants

Atsuko Kohara, Chiharu Nakajima, Shigeo Yoshida, Toshiya Muranaka

Phytochemistry　Vol. 68　No. 4　p. 478-486　2007/02　Research paper (scientific journal)

Molecular genetics of plant sterol backbone synthesis

Masashi Suzuki, Toshiya Muranaka

Lipids　Vol. 42　No. 1　p. 47-54　2007/02　Research paper (international conference proceedings)

The Outline and Significance of the Resist Wall Experiment: Role of Microtubule-Membrane-Cell Wall Continuum in Gravity Resistance in Plants

Hoson Takayuki, Matsumoto Shouhei, Soga Kouichi, Wakabayashi Kazuyuki, Hashimoto Takashi, Sonobe Seiji, Muranaka Toshiya, Kamisaka Seiichiro, Kamada Motoshi, Omori Katsunori, Ishioka Noriaki, Shimazu Toru

Biological Sciences in Space　Vol. 21　No. 3　p. 56-61　2007

植物ステロールの新しい生合成経路 : ラノステロール合成酵素の発見. 植物ステロールの多様性を解く鍵か?

鈴木 優志, 村中 俊哉

化学と生物　Vol. 44　No. 12　p. 806-807　2006/12/01

Exogenous plant H6H but not bacterial HCHL gene is expressed in Duboisia leichhardtii hairy roots and affects tropane alkaloid production

Laiq ur Rahman, Yoshie Kitamura, Junko Yamaguchi, Miki Mukai, Kuniko Akiyama, Hirobumi Yamamoto, Toshiya Muranaka, Toshihiko Ikenaga

Enzyme and Microbial Technology　Vol. 39　No. 6　p. 1183-1189　2006/10/03　Research paper (scientific journal)

P-392 BIOSYNTHETIC PATHWAY OF PHYTOSTEROLS VIA LANOSTEROL

Ohyama Kiyoshi, Suzuki Masashi, Xiang Ting, Seki Hikaru, Kikuchi Jun, Saito Kazuki, Muranaka Toshiya, Katsube Yuji, Kushiro Tetsuo, Shibuya Masaaki, Ebizuka Yutaka

International Symposium on the Chemistry of Natural Products　Vol. 2006　p. "P-392"　2006/07/23

DNA polymorphisms in the tetrahydrocannabinolic acid (THCA) synthase gene in "drug-type" and "fiber-type" Cannabis sativa L.

Mareshige Kojoma, Hikaru Seki, Shigeo Yoshida, Toshiya Muranaka

Forensic Science International　Vol. 159　No. 2-3　p. 132-140　2006/06/02　Research paper (scientific journal)

Study of DNA polymorphisms in the tetrahydrocannabinolic acid (THCA) synthase gene in "drug-type" and "fiber-type" Cannabis sativa L.

KOJOMA Mareshige, SEKI Hikaru, YOSHIDA Shigeo, MURANAKA Toshiya

Vol. 14　p. 123-129　2006/05/30

New trends of hairy root cultures

SEKI Hikaru, MURANAKA Toshiya

Bioscience & industry　Vol. 64　No. 5　p. 261-266　2006/05/01

Lanosterol synthase in dicotyledonous plants

Masashi Suzuki, Ting Xiang, Kiyoshi Ohyama, Hikaru Seki, Kazuki Saito, Toshiya Muranaka, Hiroaki Hayashi, Yuji Katsube, Tetsuo Kushiro, Masaaki Shibuya, Yutaka Ebizuka

Plant and Cell Physiology　Vol. 47　No. 5　p. 565-571　2006/05　Research paper (scientific journal)

植物ステロイドの糖転移酵素 : 配糖化に関わる遺伝子の解明により毒と薬の生産制御に期待

村中 俊哉, 小原 淳子

化学と生物　Vol. 44　No. 1　p. 6-7　2006/01/01

Hairy root-activation tagging: A high-throughput system for activation tagging in transformed hairy roots

Hikaru Seki, Tomoko Nishizawa, Nobukazu Tanaka, Yasuo Niwa, Shigeo Yoshida, Toshiya Muranaka

Plant Molecular Biology　Vol. 59　No. 5　p. 793-807　2005/11　Research paper (scientific journal)

A novel glucosyltransferase involved in steroid saponin biosynthesis in Solanum aculeatissimum

Atsuko Kohara, Chiharu Nakajima, Kimiko Hashimoto, Toshihiko Ikenaga, Hiroyuki Tanaka, Yukihiro Shoyama, Shigeo Yoshida, Toshiya Muranaka

Plant Molecular Biology　Vol. 57　No. 2　p. 225-239　2005/01　Research paper (scientific journal)

Analysis of the cannabisnoid synthase genes of Cannabis sativa L

KOJOMA Mareshige, MURANAKA Toshiya, YOSHIDA Shigeo, IIDA Osamu, SEKITA Setsuko, SATAKE Motoyoshi, MAKINO Yukiko

Vol. 12　p. 60-63　2004/06/30

Loss of function of 3-hydroxy-3-methylglutaryl coenzyme A reductase 1 (HMG1) in Arabidopsis leads to dwarfing, early senescence and male sterility, and reduced sterol levels

Masashi Suzuki, Yukiko Kamide, Noriko Nagata, Hikaru Seki, Kiyoshi Ohyama, Hisashi Kato, Kazuo Masuda, Shusei Sato, Tomohiko Kato, Satoshi Tabata, Shigeo Yoshida, Toshiya Muranaka

Plant Journal　Vol. 37　No. 5　p. 750-761　2004/03　Research paper (scientific journal)

Action of mevalonic acid-derived isoprenoids in arabidopsis

N Nagata, M Suzuki, S Yoshida, T Muranaka

ADVANCED RESEARCH ON PLANT LIPIDS　p. 245-248　2003　Research paper (international conference proceedings)

Mevalonic acid partially restores chloroplast and etioplast development in Arabidopsis lacking the non-mevalonate pathway

Noriko Nagata, Masashi Suzuki, Shigeo Yoshida, Toshiya Muranaka

Planta　Vol. 216　No. 2　p. 345-350　2002/12/01　Research paper (scientific journal)

Amyloplast formation in cultured tobacco BY-2 cells requires a high cytokinin content

Yutaka Miyazawa, Hisashi Kato, Toshiya Muranaka, Shigeo Yoshida

Plant and Cell Physiology　Vol. 43　No. 12　p. 1534-1541　2002/12/01　Research paper (scientific journal)

Determination of aculeatisides based on immunoassay using a polyclonal antibody against aculeatiside A

Waraporn Putalun, Hiroyuki Tanaka, Toshiya Muranaka, Yukihiro Shoyama

Analyst　Vol. 127　No. 10　p. 1328-1332　2002/10　Research paper (scientific journal)

Approaches to unique technologies for regulation of plant growth functions

Shigeo Yoshida, Toshiya Muranaka

Nippon Nogeikagaku Kaishi　Vol. 76　No. 1　p. 37-41　2002

A novel orfB-related gene of carrot mitochondrial genomes that is associated with homeotic cytoplasmic male sterility (CMS)

Yuki Nakajima, Toshiya Yamamoto, Toshiya Muranaka, Kenji Oeda

Plant Molecular Biology　Vol. 46　No. 1　p. 99-107　2001　Research paper (scientific journal)

微細藻類による二酸化炭素の固定と有効利用 (特集 環境・安全関連)

村中 俊哉, 西出 哲也, 村上 仁一

住友化学　Vol. 2000　No. 2　p. 19-25　2000/11　Research paper (scientific journal)

Function of a protein kinase on the regulation of both sugar and lipid metabolism in plants.

Muranaka Toshiya

Chemical Regulation of Plants　Vol. 35　No. 1　p. 17-24　2000

Regulatory interaction of PRL1 WD protein with Arabidopsis SNF1-like protein kinases

Rishikesh P. Bhalerao, Klaus Salchert, László Bakó, László Ökrész, László Szabados, Toshiya Muranaka, Yasunori Machida, Jeff Schell, Csaba Koncz

Proceedings of the National Academy of Sciences of the United States of America　Vol. 96　No. 9　p. 5322-5327　1999/04　Research paper (scientific journal)

Genetic variation of petaloid male-sterile cytoplasm of carrots revealed by sequence-tagged sites (STSs)

Y. Nakajima, T. Yamamoto, T. Muranaka, K. Oeda

Theoretical and Applied Genetics　Vol. 99　No. 5　p. 837-843　1999　Research paper (scientific journal)

The biological CO<inf>2</inf> fixation using Chlorella sp. with high capability in fixing CO<inf>2</inf>

M. Murakami, F. Yamada, T. Nishide, T. Muranaka, N. Yamaguchi, Y. Takimoto

Studies in Surface Science and Catalysis　Vol. 114　p. 315-320　1998　Research paper (scientific journal)

Over-expressed effect of carbonic anhydrase on CO<inf>2</inf> fixation in cyanobacterium, Synechococcus sp. PCC7942

M. Murakami, N. Yamaguchi, T. Nishide, T. Muranaka, Y. Takimoto

Studies in Surface Science and Catalysis　Vol. 114　p. 629-632　1998　Research paper (scientific journal)

Fungal and bacterial disease resistance in transgenic plants expressing human lysozyme

H. Nakajima, T. Muranaka, F. Ishige, K. Akutsu, K. Oeda

Plant Cell Reports　Vol. 16　No. 10　p. 674-679　1997/07　Research paper (scientific journal)

Regulation of the Mevalonate Pathway

MURANAKA Toshiya

Chemical Regulation of Plants　Vol. 32　No. 2　p. 150-159　1997

Growth and steroidal saponin production in hairy root cultures of Solanum aculeatissimum

Toshihiko Ikenaga, Toshiyuki Oyama, Toshiya Muranaka

Plant Cell Reports　Vol. 14　No. 7　p. 413-417　1995/04　Research paper (scientific journal)

A model for a bioconversion system with the promoter of the parAt gene, which confers a high level of expression of a transgene in hairy roots

Toshiya Muranaka, Yasuo Niwa, Yasunori Machida

Applied Microbiology and Biotechnology　Vol. 40　No. 6　p. 841-845　1994/02　Research paper (scientific journal)

Organ-specific and auxin-inducible expression of two tobacco par A-related genes in transgenic plants

Yasuo Niwa, Toshiya Muranaka, Akiko Baba, Yasunori Machida

DNA Research　Vol. 1　No. 5　p. 213-222　1994　Research paper (scientific journal)

Characterization of tobacco protein kinase NPK5, a homolog of Saccharomyces cerevisiae SNF1 that constitutively activates expression of the glucose-repressible SUC2 gene for a secreted invertase of S. cerevisiae

T. Muranaka, H. Banno, Y. Machida

Molecular and Cellular Biology　Vol. 14　No. 5　p. 2958-2965　1994　Research paper (scientific journal)

Continuous production of scopolamine by a culture of Duboisia leichhardtii hairy root clone in a bioreactor system

Toshiya Muranaka, Hideo Ohkawa, Yasuyuki Yamada

Applied Microbiology and Biotechnology　Vol. 40　No. 2-3　p. 219-223　1993/11　Research paper (scientific journal)

ISOLATION AND CHARACTERIZATION OF CDNA CLONES THAT ENCODE PROTEIN-KINASES OF NICOTIANA-TABACUM

H BANNO, T MURANAKA, Y ITO, T MORIBE, S USAMI, K HINATA, Y MACHIDA

JOURNAL OF PLANT RESEARCH　Vol. Special Issue 3　p. 181-192　1993/07　Research paper (scientific journal)

Characteristics of Scopolamine-releasing Hairy Root Clones of Duboisia leichhardtii

Toshiya Muranaka, Toru Kazuoka, Hideo Ohkawa, Yasuyuki Yamada

Bioscience, Biotechnology and Biochemistry　Vol. 57　No. 8　p. 1398-1399　1993　Research paper (scientific journal)

Scopolamine release into media by Duboisia leichhardtii hairy root clones

Toshiya Muranaka, Hideo Ohkawa, Yasuyuki Yamada

Applied Microbiology and Biotechnology　Vol. 37　No. 5　p. 554-559　1992/08　Research paper (scientific journal)

Organization and characterization of the virCD genes from Agrobacterium rhizogenes

Takashi Hirayama, Toshiya Muranaka, Hideo Ohkawa, Atsuhiro Oka

MGG Molecular &amp; General Genetics　Vol. 213　No. 2-3　p. 229-237　1988/08　Research paper (scientific journal)