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Expression of an endo-rhamnogalacturonase from Aspergillus aculeatus enhances release of Arabidopsis transparent mucilage.
Takao Ohashi, Yurika Mabira, Yutaro Mitsuyoshi, Hiroyuki Kajiura, Ryo Misaki, Takeshi Ishimizu, Kazuhito Fujiyama
Journal of bioscience and bioengineering 2024年4月19日 研究論文(学術雑誌)
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Recent advances in plant-based bioproduction
Kazuhito Fujiyama, Toshiya Muranaka, Atsushi Okazawa, Hikaru Seki, Goro Taguchi, Shuhei Yasumoto
Journal of Bioscience and Bioengineering 2024年4月 研究論文(学術雑誌)
出版者・発行元:Elsevier BV
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The N-linked glycosylation modifications in the hepatitis B surface protein impact cellular autophagy, HBV replication, and HBV secretion.
Patcharin Tepjanta, Kazuhito Fujiyama, Ryo Misaki, Ingorn Kimkong
PloS one Vol. 19 No. 3 2024年 研究論文(学術雑誌)
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Reconstitution of (1→3)-β-D-glucans measurement system using recombinant Limulus polyphemus Factor G
Yotaro Yamamoto, Hiroyuki Kajiura, Hiroki Fukuchi, Takahiro Nishibu, Kazuhito Fujiyama, Takeshi Kitagawa
Applied Microbiology and Biotechnology Vol. 107 No. 24 p. 7463-7473 2023年10月26日 研究論文(学術雑誌)
出版者・発行元:Springer Science and Business Media LLC
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Production and N-glycan engineering of Varlilumab in Nicotiana benthamiana
Kim Dua Nguyen, Hiroyuki Kajiura, Ryo Kamiya, Takahiro Yoshida, Ryo Misaki, Kazuhito Fujiyama
Frontiers in Plant Science Vol. 14 2023年8月8日 研究論文(学術雑誌)
出版者・発行元:Frontiers Media SA
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High accumulation of the Man5GlcNAc2 structure by combining N-acetylglucosaminyltransferase I gene suppression and mannosidase I gene overexpression in Nicotiana tabacum SR1
Keigo Sato, Hitomi Yumioka, Junko Isoyama, Koji Dohi, Akihiro Yamanaka, Takao Ohashi, Ryo Misaki, Kazuhito Fujiyama
Journal of Bioscience and Bioengineering Vol. 136 No. 2 p. 136-141 2023年8月 研究論文(学術雑誌)
出版者・発行元:Elsevier BV
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High accumulation of the Man5GlcNAc2 structure by combining N-acetylglucosaminyltransferase I gene suppression and mannosidase I gene overexpression in Nicotiana tabacum SR1.
Keigo Sato, Hitomi Yumioka, Junko Isoyama, Koji Dohi, Akihiro Yamanaka, Takao Ohashi, Ryo Misaki, Kazuhito Fujiyama
Journal of bioscience and bioengineering Vol. 136 No. 2 p. 136-141 2023年8月 研究論文(学術雑誌)
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Production and N-glycan engineering of Varlilumab in Nicotiana benthamiana.
Kim Dua Nguyen, Hiroyuki Kajiura, Ryo Kamiya, Takahiro Yoshida, Ryo Misaki, Kazuhito Fujiyama
Frontiers in plant science Vol. 14 p. 1215580-1215580 2023年 研究論文(学術雑誌)
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Effects of various disaccharide adaptations on recombinant IgA1 production in CHO-K1 suspension cells
John Benson D. Choa, Tadahiro Sasaki, Hiroyuki Kajiura, Kazuyoshi Ikuta, Kazuhito Fujiyama, Ryo Misaki
Cytotechnology 2023年 研究論文(学術雑誌)
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Insights into the quality of recombinant proteins produced by two different Bombyx mori expression systems.
Hiroyuki Kajiura, Ken-Ichiro Tatematsu, Tsuyoshi Nomura, Mitsuhiro Miyazawa, Akihiro Usami, Toshiki Tamura, Hideki Sezutsu, Kazuhito Fujiyama
Scientific reports Vol. 12 No. 1 p. 18502-18502 2022年11月2日 研究論文(学術雑誌)
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Impact of glycoengineering and antidrug antibodies on the anticancer activity of a plant-made lectin-Fc fusion protein.
Matthew Dent, Katarina L Mayer, Noel Verjan Garcia, Haixun Guo, Hiroyuki Kajiura, Kazuhito Fujiyama, Nobuyuki Matoba
Plant biotechnology journal Vol. 20 No. 11 p. 2217-2230 2022年11月 研究論文(学術雑誌)
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Mamestra brassicae NIAS-Mb-32 cell strain 2g2 enables high-yield recombinant protein production in baculovirus-free and baculovirus-based insect cell expression.
Yotaro Yamamoto, Hiroyuki Kajiura, Takahiro Nishibu, Kazuhito Fujiyama
Journal of bioscience and bioengineering Vol. 134 No. 5 p. 432-440 2022年11月 研究論文(学術雑誌)
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High-level transient production of a protease-resistant mutant form of human basic fibroblast growth factor in Nicotiana benthamiana leaves.
Edjohn Aaron Macauyag, Hiroyuki Kajiura, Takao Ohashi, Ryo Misaki, Kazuhito Fujiyama
Plant biotechnology (Tokyo, Japan) Vol. 39 No. 3 p. 291-301 2022年9月25日 研究論文(学術雑誌)
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Heterologous fusion gene expression and characterization of a novel carbohydrate binding module (Cbm36) to laccase (Lcc2)
Anita Kurniati, Ni Nyoman Tri Puspaningsih, Kartika Dwi Asni Putri, Mamik Damayanti, Ni Nyoman Purwani, Sylvia Aulia Rahmah, Purkan, Kazuhito Fujiyama, Makiko Sakka, Kazuo Sakka, Tetsuya Kimura, Ali Rohman, Afaf Baktir, Rahmat Eko Sanjaya
Biocatalysis and Agricultural Biotechnology Vol. 42 p. 102377-102377 2022年7月 研究論文(学術雑誌)
出版者・発行元:Elsevier BV
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Production of recombinant β-glucocerebrosidase in wild-type and glycoengineered transgenic Nicotiana benthamiana root cultures with different N-glycan profiles
Naphatsamon Uthailak, Hiroyuki Kajiura, Ryo Misaki, Kazuhito Fujiyama
Journal of Bioscience and Bioengineering Vol. 133 No. 5 p. 481-488 2022年5月 研究論文(学術雑誌)
出版者・発行元:Elsevier BV
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Effect of fruit maturation on N-glycosylation of plant-derived native and recombinant miraculin
Hiroyuki Kajiura, Kyoko Hiwasa-Tanase, Hiroshi Ezura, Kazuhito Fujiyama
Plant Physiology and Biochemistry Vol. 178 p. 70-79 2022年5月 研究論文(学術雑誌)
出版者・発行元:Elsevier BV
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Temporal analysis of N-acetylglucosamine extension of N-glycans in the middle silk gland of silkworm Bombyx mori.
Hiroyuki Kajiura, Takanori Eguchi, Keiro Uchino, Ken-Ichiro Tatematsu, Toshiki Tamura, Hideki Sezutsu, Kazuhito Fujiyama
Journal of bioscience and bioengineering Vol. 133 No. 6 p. 533-540 2022年4月6日 研究論文(学術雑誌)
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Establishment of serum-free adapted Chinese hamster ovary cells with double knockout of GDP-mannose-4,6-dehydratase and GDP-fucose transporter.
Ryo Misaki, Masashi Iwasaki, Hiroki Takechi, Noriko Yamano-Adachi, Takao Ohashi, Hiroyuki Kajiura, Kazuhito Fujiyama
Cytotechnology Vol. 74 No. 1 p. 163-179 2022年2月 研究論文(学術雑誌)
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Biochemical characterization of Arabidopsis clade F polygalacturonase shows a substrate preference toward oligogalacturonic acids
Takao Ohashi, Nabilah Sari, Ryo Misaki, Kazuhito Fujiyama
Journal of Bioscience and Bioengineering Vol. 133 No. 1 p. 1-7 2022年1月 研究論文(学術雑誌)
出版者・発行元:Elsevier BV
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Analysis of N-glycan profile of Arabidopsis alg3 cell culture.
Ratna Sariyatun, Hiroyuki Kajiura, Juthamard Limkul, Ryo Misaki, Kazuhito Fujiyama
Plant biotechnology (Tokyo, Japan) Vol. 38 No. 4 p. 463-467 2021年12月25日 研究論文(学術雑誌)
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Bombyx mori β1,4-N-acetylgalactosaminyltransferase possesses relaxed donor substrate specificity in N-glycan synthesis
Hiroyuki Kajiura, Ryousuke Miyauchi, Akemi Kakudo, Takao Ohashi, Ryo Misaki, Kazuhito Fujiyama
Scientific Reports Vol. 11 No. 1 2021年12月 研究論文(学術雑誌)
出版者・発行元:Springer Science and Business Media LLC
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Structure–function studies of ultrahigh molecular weight isoprenes provide key insights into their biosynthesis
Hiroyuki Kajiura, Takuya Yoshizawa, Yuji Tokumoto, Nobuaki Suzuki, Shinya Takeno, Kanokwan Jumtee Takeno, Takuya Yamashita, Shun-ichi Tanaka, Yoshinobu Kaneko, Kazuhito Fujiyama, Hiroyoshi Matsumura, Yoshihisa Nakazawa
Communications Biology Vol. 4 No. 1 2021年12月 研究論文(学術雑誌)
出版者・発行元:Springer Science and Business Media LLC
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Improved Assay System for Acidic Peptide: N-glycanase (aPNGase) Activity in Plant Extracts
Chiharu YAMAMOTO, Mikako OGURA, Ryota UEMURA, Megumi MAEDA, Hiroyuki KAJIURA, Ryo MISAKI, Kazuhito FUJIYAMA, Yoshinobu KIMURA
Anal Biochem. p. 114367-114367 2021年9月 研究論文(学術雑誌)
出版者・発行元:Elsevier BV
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Production of Human Acid-Alpha Glucosidase With a Paucimannose Structure by Glycoengineered Arabidopsis Cell Culture
Ratna Sariyatun, Florence, Hiroyuki Kajiura, Takao Ohashi, Ryo Misaki, Kazuhito Fujiyama
Frontiers in Plant Science Vol. 12 2021年7月14日 研究論文(学術雑誌)
出版者・発行元:Frontiers Media SA
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Transient Production of Human β-Glucocerebrosidase With Mannosidic-Type N-Glycan Structure in Glycoengineered Nicotiana benthamiana Plants
Naphatsamon Uthailak, Hiroyuki Kajiura, Ryo Misaki, Kazuhito Fujiyama
Frontiers in Plant Science Vol. 12 2021年6月7日 研究論文(学術雑誌)
出版者・発行元:Frontiers Media SA
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Direct evidence of cytosolic PNGase activity in Arabidopsis thaliana: in vitro assay system for plant cPNGase activity
Sahoko Shirai, Ryota Uemura, Megumi Maeda, Hiroyuki Kajiura, Ryo Misaki, Kazuhito Fujiyama, Yoshinobu Kimura
Bioscience, Biotechnology, and Biochemistry Vol. 85 No. 6 p. 1460-1463 2021年5月25日 研究論文(学術雑誌)
出版者・発行元:Oxford University Press (OUP)
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Structure and Biological Functions of Plant Glycans and Polysaccharides
Ryo Misaki, Kazuhito Fujiyama, Hiroyuki Kajiura
Comprehensive Glycoscience p. 93-109 2021年 論文集(書籍)内論文
出版者・発行元:Elsevier
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Functional characterization and overexpression of Δ12-desaturase in the oleaginous yeast Rhodotorula toruloides for production of linoleic acid-rich lipids
Chih Chan Wu, Takao Ohashi, Hiroyuki Kajiura, Yu Sato, Ryo Misaki, Kohsuke Honda, Savitree Limtong, Kazuhito Fujiyama
Journal of Bioscience and Bioengineering 2021年 研究論文(学術雑誌)
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Transglycosylation toward naringenin-7-O-glucoside using an N180H mutant of Coprinopsis cinerea endo-β-N-acetylglucosaminidase
Takao Ohashi, Yu Fujisawa, Marc R. Hayes, Ryo Misaki, Jörg Pietruszka, Kazuhito Fujiyama
Biochemical and Biophysical Research Communications Vol. 530 No. 1 p. 155-159 2020年9月10日 研究論文(学術雑誌)
出版者・発行元:Elsevier B.V.
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Characterization of Bombyx mori N-acetylglucosaminyltransferase II splicing variants
Hiroyuki Kajiura, Yugo Nakamura, Mari Nishimura, Takao Ohashi, Ryo Misaki, Kazuhito Fujiyama
Biochemical and Biophysical Research Communications Vol. 529 No. 2 p. 404-410 2020年8月 研究論文(学術雑誌)
出版者・発行元:Elsevier BV
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Enhancement of sialylation in rIgG in glyco-engineered Chinese hamster ovary cells
Nguyen TS, Misaki R, Ohashi T, Fujiyama K
Cytotechnology Vol. 72 No. 3 p. 343-355 2020年7月 研究論文(学術雑誌)
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Ethanol and H2O2 stresses enhance lipid production in an oleaginous Rhodotorula toruloides thermotolerant mutant L1-1.
Chih-Chan Wu, Takao Ohashi, Ryo Misaki, Savitree Limtong, Kazuhito Fujiyama
FEMS yeast research Vol. 20 No. 4 2020年6月1日 研究論文(学術雑誌)
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Fully Human Monoclonal Antibodies Effectively Neutralizing Botulinum Neurotoxin Serotype B
Matsumura T, Amatsu S, Misaki R, Yutani M, Anariwa D, Kohda T, Fujiyama K, Ikuta K, Fujinaga Y
Toxins Vol. 12 No. 5 2020年5月 研究論文(学術雑誌)
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Mating-type switching and mating-type gene array expression in the methylotrophic yeast Ogataea thermomethanolica TBRC656
Wongwisansri S, Promdonkoy P, Likhitrattanapisal S, Harnpichanchai P, Fujiyama K, Kaneko Y, Eurwilaichitr L, Ingsrisawang S, Tanapongpipat S
Microbiological Resarch Vol. 232 2020年2月 研究論文(学術雑誌)
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Biosynthetic Pathway of Indole-3-Acetic Acid in Basidiomycetous Yeast Rhodosporidiobolus fluvialis
Bunsangiam S, Sakpuntoon V, Srisuk N, Ohashi T, Fujiyama K, Limtong S
Mycobiology Vol. 47 No. 3 p. 292-300 2019年7月 研究論文(学術雑誌)
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Development of a Paper-Based Luminescence Bioassay for Therapeutic Monitoring of Aminoglycosides: a Proof-of-Concept Study.
Matsuura H, Ujiie K, Duyen TTM, Izutsu K, Fujiyama K, Maeda S, Harada K, Hirata K
Applied Biochemistry and Biotechnology 2019年5月 研究論文(学術雑誌)
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The neutral N-linked glycans of the Basidiomycetous yeasts Pseudozyma antarctica and Malassezia furfur (Subphylum Ustilaginomycotina)
Flores RJD, Ohashi T, Sakai K, Gonoi T, Kawasaki H, Fujiyama K
J Gen Appl Microbiol Vol. 65 No. 2 p. 53-63 2019年5月 研究論文(学術雑誌)
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Delta-9 fatty acid desaturase overexpression enhanced lipid production and oleic acid content in Rhodosporidium toruloides for preferable yeast lipid production
Tsai YY, Ohashi T, Wu CC, Bataa D, Misaki R, Limtong S, Fujiyama K
J Biosci Bioeng Vol. 127 No. 4 p. 430-440 2019年4月 研究論文(学術雑誌)
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Technologies to elucidate functions of glycans
Koichi Furukawa, Yuhsuke Ohmi, Yuji Kondo, Yuki Ohkawa, Orie Tajima, Keiko Furukawa, Koichi Honke, Jin Ichi Inokuchi, Jianguo Gu, Kenji Kadomatsu, Satomi Nadanaka, Hiroshi Kitagawa, Shoko Nishihara, Kazuya Nomura, Shogo Oka, Makoto Ito, Ken Kitajima, Shunji Natsuka, Motoi Kanagawa, Takeshi Ishimizu, Kazuhito Fujiyama, Yasunori Chiba, Hiroyuki Osada
Glycoscience: Basic Science to Applications: Insights from the Japan Consortium for Glycobiology and Glycotechnology (JCGG) p. 87-124 2019年1月1日 論文集(書籍)内論文
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Arabidopsis thaliana α1,2-L-fucosyltransferase catalyzes the transfer of L-galactose to xyloglucan oligosaccharide
Ohashi H, Ohashi T, Misaki R, Fujiyama K
FEBS Lett 2019年1月 研究論文(学術雑誌)
-
Transcriptome sequencing and identification of cytochrome P450 monooxygenases involved in the biosynthesis of maslinic acid and corosolic acid in Avicennia marina.
Mitsuki Nakamura, Tran My Linh, Le Quynh Lien, Hayato Suzuki, Nguyen Chi Mai, Vu Huong Giang, Keita Tamura, Nguyen Van Thanh, Hideyuki Suzuki, Ryo Misaki, Toshiya Muranaka, Ninh Khac Ban, Kazuhito Fujiyama, Hikaru Seki
Plant biotechnology (Tokyo, Japan) Vol. 35 No. 4 p. 341-348 2018年12月25日 研究論文(学術雑誌)
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Comparison of the N-glycosylation on recombinant miraculin expressed in tomato plants with native miraculin
Tanase, Kyoko, Ezura, Hiroshi, Kajiura, Hiroyuki, Fujiyama, Kazuhito
Plant Biotechnology 2018年12月 研究論文(学術雑誌)
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St6gal1 knockdown alters HBV life cycle in HepAD38 cells
Priyambada SA, Misaki R, Okamoto T, Ohashi T, Ueda K, Matsuura Y, Fujiyama K
Biochem Biophys Res Commun Vol. 503 No. 3 p. 1841-1847 2018年9月 研究論文(学術雑誌)
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Isolation of a thermotolerant Rhodosporidium toruloides DMKU3-TK16 mutant and its fatty acid profile at high temperature
Wu CC, Tsai YY, Ohashi T, Misaki R, Limtong S, Fujiyama K
FEMS Microbiol Lett Vol. 365 No. 21 2018年8月 研究論文(学術雑誌)
-
N-glycan sialylation in a silkworm-baculovirus expression system
Masatoshi Suganuma, Tsuyoshi Nomura, Yukiko Higa, Yukiko Kataoka, Shunsuke Funaguma, Hironobu Okazaki, Takeo Suzuki, Kazuhito Fujiyama, Hideki Sezutsu, Ken ichiro Tatematsu, Toshiki Tamura
Journal of Bioscience and Bioengineering Vol. 126 No. 1 p. 9-14 2018年7月 研究論文(学術雑誌)
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The production of human β-glucocerebrosidase in Nicotiana benthamiana root culture
Naphatsamon U, Ohashi T, Misaki R, Fujiyama K
Int J Mol Sci 2018年7月 研究論文(学術雑誌)
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Enhancement of glycosylation by stable co-expression of two sialylation-related enzymes on Chinese hamster ovary cells
Nguyen TS, Misaki R, Ohashi T, Fujiyama K
J Biosci Bioeng 2018年7月 研究論文(学術雑誌)
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Hydroponic treatment of Nicotiana benthamiana with kifunensine modifies the N-glycans of recombinant glycoprotein antigens to predominantly Man9 high-mannose type upon transient overexpression.
Roychowdhury S, Oh YJ, Kajiura H, Hamorsky KT, Fujiyama K, Matoba N
Front Plant Sci. Vol. 9 p. 62-62 2018年4月 研究論文(学術雑誌)
-
Molecular cloning and overexpression of DGA1, an acyl-CoA dependent diacylglycerol acyltransferase, in the oleaginous yeast Rhodosporidiobolus fluvialis DMKU-RK253
Polburee P, Ohashi T, Tsai YY, Sumyai T, Lertwattanasakul N, Limtong S, Fujiyama K
Microbiology 2018年1月 研究論文(学術雑誌)
-
Isolation and characterization of a stress-responsive gene encoding a CHRD domain-containing protein from a halotolerant green alga
Ryo Ishinishi, Hideyuki Matsuura, Satoshi Tanaka, Saaya Nozawa, Keisuke Tanada, Norihito Kawashita, Kazuhito Fujiyama, Hitoshi Miyasaka, Kazumasa Hirata
GENE Vol. 640 p. 14-20 2018年1月 研究論文(学術雑誌)
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Effective Synthesis of Guanosine 5’-Diphospho-β-L-galactose Using Bacterial L-Fucokinase/Guanosine 5’-diphosphate-L-fucose Pyrophosphorylase
Ohashi H, Wahl C, Ohashi T, Elling L, Fujiyama K
Adv Synth Catal 2017年10月 研究論文(学術雑誌)
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A polygalacturonase localized in the Golgi apparatus in Pisum sativum
Ohashi, T, Jinno, J, Inoue, Y, Ito, S, Fujiyama, K, Ishimizu, T
J. Biochem. Vol. 161 No. 6 2017年7月
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Cell surface N-glycan alteration in HepAD38 cell lines expressing Hepatitis B virus
Surya Agung Priyambada, Ryo Misaki, Toru Okamoto, Yuta Okamoto, Takao Ohashi, Keiji Ueda, Yoshiharu Matsuura, Kazuhito Fujiyama
Virus Research Vol. 238 p. 101-109 2017年6月15日 研究論文(学術雑誌)
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Erratum to: Development of a sufficient and effective procedure for transformation of an oleaginous yeast, Rhodosporidium toruloides DMKU3-TK16 (Current Genetics, (2017), 63, 2, (359-371), 10.1007/s00294-016-0629-8)
Yung-Yu Tsai, Takao Ohashi, Takenori Kanazawa, Pirapan Polburee, Ryo Misaki, Savitree Limtong, Kazuhito Fujiyama
Current Genetics Vol. 63 No. 2 2017年5月1日 研究論文(学術雑誌)
出版者・発行元:Springer Verlag
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Biochemical characterization of rhamnosyltransferase involved in biosynthesis of pectic rhamnogalacturonan I in plant cell wall
Uehara Y, Tamura S, Maki Y, Yagyu K, Mizoguchi T, Tamiaki H, Imai T, Ishii T, Ohashi T, Fujiyama K, Ishimizu T
Biochem Biophys Res Commun 2017年4月 研究論文(学術雑誌)
-
The neutral N-linked glycans of the Ustilaginomycetes yeast Sympodiomycopsis pahiopedili
Flores RJD, Ohashi T, Kawasaki H, Fujiyama K
Yeast 2017年4月 研究論文(学術雑誌)
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Two Eucommia farnesyl diphosphate synthases exhibit distinct enzymatic properties leading to end product preferences.
Kajiura H, Suzuki N, Tokumoto Y, Yoshizawa T, Takeno S, Fujiyama K, Kaneko Y, Matsumura H, Nakazawa Y
Biochimie Vol. 139 p. 95-106-106 2017年4月 研究論文(学術雑誌)
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N-glycan structures and downstream mannose-phosphorylation of plant recombinant human alpha-L-iduronidase: toward development of enzyme replacement therapy for mucopolysaccharidosis I.
Pierce OM, McNair GR, He X, Kajiura H, Fujiyama K, Kermode AR
Plant Mol Biol. Vol. 95 No. 6 p. 593-606-606 2017年4月 研究論文(学術雑誌)
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A polygalacturonase localized in the Golgi apparatus in Pisum sativum
Ohashi T, Jinno J, Inoue Y, Ito S, Fujiyama K, Ishimizu T
J Biochem 2017年2月 研究論文(学術雑誌)
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Fucosyltransferases produce N-glycans containing core L-galactose
Ohashi H, Ohashi T, Kajiura H, Misaki R, Kitamura S, Fujiyama K
Biochem Biophys Res Commun Vol. 483 No. 1 p. 658-663 2017年1月 研究論文(学術雑誌)
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The production of human glucocerebrosidase in glyco-engineered Nicotiana benthamiana plants.
Juthamard Limkul, Sayoko Iizuka, Yohei Sato, Ryo Misaki, Takao Ohashi, Toya Ohashi, Kazuhito Fujiyama
Plant biotechnology journal Vol. 14 No. 8 p. 1682-94 2016年8月 研究論文(学術雑誌)
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Lipid production from biodiesel-derived crude glycerol by Rhodosporidium fluviale DMKU-RK253 using temperature shift with high cell density
Pirapan Polburee, Wichien Yongmanitchai, Kohsuke Honda, Takao Ohashi, Toshiomi Yoshida, Kazuhito Fujiyama, Savitree Limtong
BIOCHEMICAL ENGINEERING JOURNAL Vol. 112 p. 208-218 2016年8月 研究論文(学術雑誌)
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Development of a sufficient and effective procedure for transformation of an oleaginous yeast, Rhodosporidium toruloides DMKU3‑TK16
Tsai Y-Y, Ohashi T, Kanazawa T, Polburee P, Misaki R, Limtong S, Fujiyama K
Curr Genet 2016年7月 研究論文(学術雑誌)
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Core-fucosylation plays a pivotal role in hepatitis B pseudo virus infection: a possible implication for HBV glycotherapy
Shinji Takamatsu, Mayuka Shimomura, Yoshihiro Kamada, Haruka Maeda, Tomoaki Sobajima, Hayato Hikita, Masumi Iijima, Yuta Okamoto, Ryo Misaki, Kazuhito Fujiyama, Shushi Nagamori, Yoshikatsu Kanai, Tetsuo Takehara, Keiji Ueda, Shun'ichi Kuroda, Eiji Miyoshi
Glycobiology 2016年6月21日 研究論文(学術雑誌)
出版者・発行元:Oxford University Press (OUP)
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Altered glycosylation of exported proteins,including surface immune receptors, compromises calcium and downstream signaling responses to microbe-associated molecular patterns in Arabidopsis thaliana.
Trempel F, Kajiura H, Ranf S, Grimmer J, Westphal L, Zipfel C, Scheel D, Fujiyama K, Lee J
BMC Plant Biol. Vol. 16 2016年4月 研究論文(学術雑誌)
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Recombinant production and characterization of human anti-influenza virus monoclonal antibodies identified from hybridomas fused with human lymphocytes.
Misaki R, Fukura N, Kajiura H, Yasugi M, Kubota-Koketsu R, Sasaki T, Momota M, Ono K, Ohashi T, Ikuta K, Fujiyama K
Biologicals Vol. 44 2016年4月
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The combination of plant translational enhancers and terminator increase the expression of human glucocerebrosidase in Nicotiana benthamiana plants
Juthamard Limkul, Ryo Misaki, Ko Kato, Kazuhito Fujiyama
Plant Science Vol. 240 p. 41-49 2015年11月1日 研究論文(学術雑誌)
出版者・発行元:Elsevier Ireland Ltd
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A novel potential signal peptide sequence and overexpression of ER-resident chaperones enhance heterologous protein secretion in thermotolerant methylotrophic yeast Ogataea thermomethanolica
Roongsawang N, Puseenam A, Kitikhun S, Sae-Tang K, Harnpicharnchai P, Ohashi T, Fujiyama K, Tirasophon W, Tanapongpipat S
Appl Biochem Biotechnol 2015年10月 研究論文(学術雑誌)
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Substrate preference of citrus naringenin rhamnosyltransferases and their application to flavonoid glycoside production in fission yeast
Ohashi T, Hasegawa Y, Misaki R, Fujiyama K
Appl Microbiol Biotechnol Vol. 100 No. 2 p. 687-696 2015年10月 研究論文(学術雑誌)
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Characterization of oleaginous yeasts accumulating high levels of lipid when cultivated in glycerol and their potential for lipid production from biodiesel-derived crude glycerol
Polburee P, Yongmanitchai W, Lertwattanasakul N, Ohashi T, Fujiyama K, Limtong S
Fungal Biol 2015年9月 研究論文(学術雑誌)
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N-glycosylation of cholera toxin B subunit in Nicotiana benthamiana: impacts on host stress response, production yield and vaccine potential.
Hamorsky KT, Kouokam JC, Jurkiewicz JM, Nelson B, Moore LJ, Husk AS, Kajiura H, Fujiyama K, Matoba N
Sci Rep. Vol. 23 2015年4月 研究論文(学術雑誌)
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Im"plant"ing of mammalian glycosyltransferase gene into plant suspension-cultured cells using Agrobacterium-mediated transformation.
Kajiura H, Fujiyama K
Methods Mol Biol. Vol. 1321 p. 225-232-232 2015年4月 研究論文(学術雑誌)
出版者・発行元:
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Production of initial-stage eukaryotic N-glycan and its protein glycosylation in Escherichia coli
Srichaisupakit A, Ohashi T, Misaki R, Fujiyama K
J Biosci Bioeng Vol. 119 No. 4 p. 399-405 2015年4月 研究論文(学術雑誌)
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Sialylation potentials of the silkworm, Bombyx mori; B.mori possesses an active α2,6-sialyltransferase.
Kajiura H, Hamaguchi Y, Mizushima H, Misaki R, Fujiyama K
Glycobiology Vol. 25 No. 12 p. 1441-1453-1453 2015年4月 研究論文(学術雑誌)
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Cloning and expression analysis of acidic peptide:N-glycanase genes in Solanum lycoperisicum.
Fujikawa, M, Maeda, M, Fujiyama , K, Kimura, Y
Glycoconjugate Journal Vol. 32 No. 5 2015年
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Barnettozyma siamensis f.a., sp. nov., a lipid-accumulating ascomycete yeast species
Polburee P, Yongmanitchai W, Ohashi T, Fujiyama K, Limtong S
Int J Syst Evol Microbiol Vol. 64 No. Pt 9 p. 3053-3057 2014年9月 研究論文(学術雑誌)
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Identification of a protein glycosylation operon from Campylobacter jejuni JCM 2013 and its heterologous expression in Escherichia coli
Srichaisupakit A, Ohashi T, Fujiyama K
J Biosci Bioeng Vol. 118 No. 3 p. 256-262 2014年9月 研究論文(学術雑誌)
出版者・発行元:公益社団法人日本生物工学会
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Antibody germline characterization of cross-neutralizing human IgGs against 4 serotypes of dengue virus.
Pannamthip Pitaksajjakul, Surachet Benjathummarak, Cholnatip Pipattanaboon, Waranya Wongwit, Tamaki Okabayashi, Motoki Kuhara, Ryo Misaki, Kazuhito Fujiyama, Pongrama Ramasoota
Biochemical and Biophysical Research Communicatioins Vol. 446 No. 2 p. 475-480 2014年4月 研究論文(学術雑誌)
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植物N-結合型糖鎖修飾酵素の異種細胞における発現と修飾経路の考察
梶浦 裕之, 藤山 和仁
化学と生物 Vol. 52 No. 11 p. 719-724 2014年
出版者・発行元:Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Cloning of a cDNA encoding the Gly m Bd 28K precursor and its vacuole transport in tobacco BY2 suspension-cultured cells.
Hitomi Yumioka-Ito, Ryo Misaki, Miyuki Yokoro, Makiko Suzuki, Hiromi Yamashita, Miki Hiemori-Kondo, Masumi Kimoto, Ko Kato, Kazuhito Fujiyama, Hideaki Tsuji
Journal of nutritional science and vitaminology Vol. 60 No. 2 p. 129-39 2014年 研究論文(学術雑誌)
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Emerging Antigenic Variants at the Antigenic Site Sb in Pandemic A(H1N1)2009 Influenza Virus in Japan Detected by a Human Monoclonal Antibody
M. Yasugi, R. Kubota-Koketsu, A. Yamashita, N. Kawashita, A. Du, R. Misaki, M. Kuhara, N. Boonsathorn, K. Fujiyama, Y. Okuno, T. Nakaya, K. Ikuta
PLoS One Vol. 8 No. 10 2013年10月 研究論文(学術雑誌)
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Dengue virus neutralization and antibody-dependent enhancement activities of human monoclonal antibodies derived from dengue patients at acute phase of secondary infection.
Tadahiro Sasaki, Chayanee Setthapramote, Takeshi Kurosu, Mituhiro Nishimura, Azusa Asai, Tamaki Okabayashi, Magot Omokoko, Chonlatip Pipattanaboon, Pannnamthip Pitaksajjakul, Kriengsak, Limittikul, Arunee Subchareon, Panjaporn Chaichana, Tamaki Okabayashi, Itaru Hirai, Pornsawan Leaungwutiwong, Ryo Misaki, Kazuhiro Fujiyama, Ken-ichiro Ono, Yoshinobu Okuno, Pongrama Ramasoota, Kazuyoshi Ikuta
Antiviral Research Vol. 10 p. 1016-1016 2013年6月 研究論文(学術雑誌)
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Rapid and scalable plant-based production of a cholera toxin B subunit variant to aid in mass vaccination against cholera outbreaks.
Hamorsky KT, Kouokam JC, Bennett LJ, Baldauf KJ, Kajiura H, Fujiyama K, Matoba N
PLoS Negl Trop Dis. Vol. 7 No. 3 2013年4月 研究論文(学術雑誌)
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Transgenic Rice Seeds Accumulating Recombinant Hypoallergenic Birch Pollen Allergen Bet v 1 Generate Giant Protein Bodies.
Wang S, Takahashi H, Kajiura H, Kawakatsu T, Fujiyama K, Takaiwa F
Plant Cell Physiol. Vol. 54 2013年4月 研究論文(学術雑誌)
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N-Glycosylation and N-Glycan moieties of CTB expressed in rice seeds.
Kajiura H, Wasai M, Kasahara S, Takaiwa F, Fujiyama K
Mol Biotechnol. Vol. 54 No. 3 p. 784-794-794 2013年4月 研究論文(学術雑誌)
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Human monoclonal antibodies broadly neutralizing against influenza B virus.
Yasugi M, Kubota-Koketsu R, Yamashita A, Kawashita N, Du A, Sasaki T, Nishimura M, Misaki R, Kuhara M, Boonsathorn N, Fujiyama K, Okuno Y, Nakaya T, Ikuta K
PLoS Pathog Vol. 9 No. 2 2013年2月 研究論文(学術雑誌)
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Identification of Novel alpha 1,3-Galactosyltransferase and Elimination of alpha-Galactose-containing Glycans by Disruption of Multiple alpha-Galactosyltransferase Genes in Schizosaccharomyces pombe
Takao Ohashi, Kazuhito Fujiyama, Kaoru Takegawa
JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 287 No. 46 p. 38866-38875 2012年11月 研究論文(学術雑誌)
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Characterization of URA3 gene in oleaginous yeast Rhodosporidium toruloides DMKU3-TK16 for lipid engineering
Takenori Kanazawa, Takao Ohashi, Ayako Tomio, Ryo Misaki, Takeshi Bamba, Eiichiro Fukusaki, Limtong Savitree, Kazuhito Fujiyama
2012年10月
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Production of different glycosylation variants of the tumour-targeting mAb H10 in Nicotiana benthamiana: influence on expression yield and antibody degradation.
Lombardi R, Donini M, Villani ME, Brunetti P, Fujiyama K, Kajiura H, Paul M, Ma JK, Benvenuto E
Transgenic Res. Vol. 21 2012年4月 研究論文(学術雑誌)
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Evolutionarily conserved glycan signal to degrade aberrant brassinosteroid receptors in Arabidopsis.
Hong Z, Kajiura H, Su W, Jin H, Kimura A, Fujiyama K,Li J
Proc Natl Acad Sci U S A Vol. 109 2012年4月 研究論文(学術雑誌)
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N-Glycan structures of glycoproteins in suspension-cultured Arabidopsis thaliana T87 cells.
Kajiura H, Tanaka R, Kato K, Hamaguchi Y, Seki T, Fujiyama K
Plant Biotechnol. Vol. 29 No. 5 p. 489-493-493 2012年4月 研究論文(学術雑誌)
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Enhancement of sialylation on humanized IgG-like bispecific antibody by overexpression of alpha2,6-sialyltransferase derived from Chinese hamster ovary cells.
Onitsuka M, Kim WD, Ozaki H, Kawaguchi A, Honda K, Kajiura H, Fujiyama K, Asano R, Kumagai I, Ohtake H, Omasa T
Appl Microbiol Biotechnol. Vol. 94 No. 1 p. 69-80-80 2012年4月 研究論文(学術雑誌)
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Arabidopsis beta 1,2-xylosyltransferase: Substrate specificity and participation in the plant-specific N-glycosylation pathway
Hiroyuki Kajiura, Toru Okamoto, Ryo Misaki, Yoshiharu Matsuura, Kazuhito Fujiyama
JOURNAL OF BIOSCIENCE AND BIOENGINEERING Vol. 113 No. 1 p. 48-54 2012年1月 研究論文(学術雑誌)
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N-terminal vacuolar sorting signal at the mouse antibody alters the N-linked glycosylation pattern in suspension-cultured tobacco BY2 cells
Ryo Misaki, Yohei Sakai, Takeshi Omasa, Kazuhito Fujiyama, Tatsuji Seki
JOURNAL OF BIOSCIENCE AND BIOENGINEERING Vol. 112 No. 5 p. 476-484 2011年11月 研究論文(学術雑誌)
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Improved expression and characterization of recombinant human Golgi alpha 1, 2-mannosidase I isoforms (IA2 and IC) by Escherichia coli
Junko Isoyama-Tanaka, Koji Dohi, Ryo Misaki, Kazuhito Fujiyama
JOURNAL OF BIOSCIENCE AND BIOENGINEERING Vol. 112 No. 1 p. 14-19 2011年7月 研究論文(学術雑誌)
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Reduced Immunogenicity of Arabidopsis hgl1 Mutant N-Glycans Caused by Altered Accessibility of Xylose and core Fucose Epitopes
Heidi Kaulfuerst-Soboll, Stephan Rips, Hisashi Koiwa, Hiroyuki Kajiura, Kazuhito Fujiyama, Antje von Schaewen
JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 286 No. 26 p. 22955-22964 2011年7月 研究論文(学術雑誌)
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Double-Knockout of Putative Endo-beta-N-acetylglucosaminidase (ENGase) Genes in Arabidopsis thaliana: Loss of ENGase Activity Induced Accumulation of High-Mannose Type Free N-Glycans Bearing N,N '-Acetylchitobiosyl Unit
Yoshinobu Kimura, Yuki Takeoka, Masami Inoue, Megumi Maeda, Kazuhito Fujiyama
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY Vol. 75 No. 5 p. 1019-1021 2011年5月 研究論文(学術雑誌)
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Reduced immunogenicity of Arabidopsis hybrid glycosylation1 (hgl1) N-glycans due to altered accessibility of xylose and core fucose epitopes.
Kaulfuerst-Soboll H, Rips S, Koiwa H, Kajiura H, Fujiyama K, von Schaewen A
J Biol Chem. Vol. 286 2011年4月 研究論文(学術雑誌)
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Stable coexpression of two human sialylation enzymes in plant suspension-cultured tobacco cells.
Kajiura H, Misaki R, Fujiyama K, Seki T
J Biosci Bioeng. Vol. 111 No. 4 p. 471-477-477 2011年4月 研究論文(学術雑誌)
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Jack bean alpha-mannosidase digestion profile of hybrid-type N-glycans: Effect of reaction pH on substrate preference
Koji Dohi, Junko Isoyama-Tanaka, Ryo Misaki, Kazuhito Fujiyama
BIOCHIMIE Vol. 93 No. 4 p. 766-771 2011年4月 研究論文(学術雑誌)
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P-62 極限環境を生み出すインテリジェント栽培装置の開発(ポスターセッション1:資源1,研究発表(ポスター発表))
林 潤, 武石 裕行, 中塚 記章, 須田 慶人, 町村 尚, 岡澤 敦司, 原田 和生, 平田 収正, 藤山 和仁, 小林 昭雄, 赤松 史光
バイオマス科学会議発表論文集 Vol. 6 p. 176-177 2011年
出版者・発行元:一般社団法人日本エネルギー学会
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Rice Golgi proteome : identification and characterization of N-acetylglucosaminyltransferase-I-like protein.
Mitsui T, Asakura T, Nakayama Y, Takayama K, Hirose S, Katamine H, Aoyama M, Karahashi A, Kaneko K, Ishiyama R, Itoh K, Hori H, Kajiura H, Fujiyama K
Bull. Facul. Agric. Niigata Univ. Vol. 63 2010年4月 研究論文(学術雑誌)
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The impact of the overexpression of human UDP-galactose transporter gene hUGT1 in tobacco plants.
Khalil MF, Kajiura H, Fujiyama K, Koike K, Ishida N, Tanaka N
J Biosci Bioeng. Vol. 109 2010年4月 研究論文(学術雑誌)
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Arabidopsis thaliana ALG3 mutant synthesizes immature oligosaccharides in the ER and accumulates unique N-glycans.
Kajiura H, Seki T, Fujiyama K
Glycobiology Vol. 20 No. 6 p. 736-751-751 2010年4月 研究論文(学術雑誌)
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Two Arabidopsis thaliana Golgi {alpha}-mannosidase Is are responsible for plant N-glycan maturation
Hiroyuki Kajiura, Hisashi Koiwa, Yoshihisa Nakazawa, Atsushi Okazawa, Akio Kobayashi, Tatsuji Seki, Kazuhito Fujiyama
Glycobiology Vol. 20 No. 2 p. 235-247 2010年2月 研究論文(学術雑誌)
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Wickerhamomyces edaphicus sp. nov. and Pichia jaroonii sp. nov., two ascomycetous yeast species isolated from forest soil in Thailand
Savitree Limtong, Wichien Yongmanitchai, Hiroko Kawasaki, Kazuhito Fujiyama
FEMS Yeast Research Vol. 9 No. 3 p. 504-510 2009年5月 研究論文(学術雑誌)
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Biochemical and immunological characterization of the plant-derived candidate human immunodeficiency virus type 1 mucosal vaccine CTB-MPR.
Matoba N, Kajiura H, Cherni I, Doran JD, Bomsel M, Fujiyama K, Mor TS
Plant Biotechnol J. Vol. 7 2009年4月 研究論文(学術雑誌)
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Cloning and characterization of cytidine monophosphate-3-deoxy-D-manno-octulosonate synthetase from Arabidopsis thaliana.
Misaki R, Kajiura H, Fujii K, Fujiyama K, Seki T
J Biosci Bioeng. Vol. 108 No. 6 p. 527-529-529 2009年4月 研究論文(学術雑誌)
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Molecular Cloning and Gene Expression Analysis of Tomato Endo-beta-N-acetylglucosaminidase, an Endoglycosidase Involved in the Production of High-Mannose Type Free N-Glycans during Tomato Fruit Ripening
Kosuke Nakamura, Masami Inoue, Megumi Maeda, Ryohei Nakano, Katsutoshi Hosoi, Kazuhito Fujiyama, Yoshinobu Kimura
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY Vol. 73 No. 2 p. 461-464 2009年2月 研究論文(学術雑誌)
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Change in glycosylation pattern with extension of endoplasmic reticulum retention signal sequence of mouse antibody produced by suspension-cultured tobacco BY2 cells
Kazuhito Fujiyama, Ryo Misaki, Yohei Sakai, Takeshi Omasa, Tatsuji Seki
JOURNAL OF BIOSCIENCE AND BIOENGINEERING Vol. 107 No. 2 p. 165-172 2009年2月 研究論文(学術雑誌)
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Analysis of CMP-sialic acid transporter-like proteins in plants
Shou Takashima, Junichi Seino, Takeshi Nakano, Kazuhito Fujiyama, Masafumi
Phytochemistry Vol. 70 p. 1973-1981 2009年1月 研究論文(学術雑誌)
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Generation of a transgenic rice seed-based edible vaccine against house dust mite allergy.
Yang L, Kajiura H, Suzuki K, Hirose S, Fujiyama K, Takaiwa F
Biochem Biophys Res Commun. Vol. 365 2008年4月 研究論文(学術雑誌)
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Salt tolerance of Arabidopsis thaliana requires maturation of N-glycosylated proteins in the Golgi apparatus.
Kang JS, Frank J, Kang CH, Kajiura H, Vikram M, Ueda A, Kim S, Bahk JD, Triplett B, Fujiyama K, Lee SY, von, Schaewen A, Koiwa H
Proc Natl Acad Sci U S A Vol. 105 p. 5933-5938-5938 2008年4月 研究論文(学術雑誌)
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Salt tolerance of Arabidopsis thaliana requires maturation of N-glycosylated proteins in the Golgi
J. S. Kang, J. Frank, C. H. Kang, H. Kajiura, M. Vikram, A. Ueda, S. Kim, J. D. Bahk, B. Triplett, K. Fujiyama, S. Y. Lee, A. von Schaewen, H.i Koiwa
Proc Natl Acad Sci USA. Vol. 105 No. 15 p. 5933-8 2008年4月 研究論文(学術雑誌)
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Biochemical and immunological characterization of the plant-derived candidate human immunodeficiency virus type 1 mucosal vaccine CTB-MPR649-684
Nobuyuki Matoba, Hiroyuki Kajiura, Irene Cherni, Jeffrey D Doran, Morgane Bomsel, Kazuhito Fujiyama, Tsafrir S Mor
Plant Biotechnol J 2008年2月 研究論文(学術雑誌)
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Salt-adaptation of Tobacco BY2 Cells Induces Change in Glycoform of N-Glycans: Enhancement of Exo- and Endo-glycosidase Activities by Salt-adaptation.
Y. Kimura, T. Watanabe, M. Maeda, Y. Murata, K. Fujiyama
Biosci. Biotechnol. Biochem. Vol. 72 No. 2 p. 514-522 2008年2月 研究論文(学術雑誌)
出版者・発行元:Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Generation of a transgenic rice seed-based edible vaccine against house dust mite allergy
L. Yang, H. Kajiura, K. Suzuki, S. Hirose, K. Fujiyama, F. Takaiwa
Biochem. Biophys. Res. Commun. 2007年10月 研究論文(学術雑誌)
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Molecular Cloning and Expression of Two Novel beta-N-acetylglucosaminidases from Silkworm Bombyx mori
T. Okada, S. Ishiyama, H. Sezutsu, A. Usami, T. Tamura, K. Mita, K. Fujiyama, T. Seki
Biosci. Biotechnol. Biochem. Vol. 71 No. 7 p. 1626-1635 2007年7月 研究論文(学術雑誌)
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First Evidence for 1-3 Galactosyltransferase Activity Involved in Biosynthesis of Insect Complex Type N-Glycan Harboring T-Antigen in Apis mellifera
Y. Kimura, S. Sakamura, T. Ushijima, Y. Hama, H. Kajiura, K. Fujiyama, K. Okihara, K. Hashimoto, H
2007年4月 研究論文(学術雑誌)
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Evidence for new beta1-3 galactosyltransferase activity involved in biosynthesis of unusual N-glycan harboring T-antigen in Apis mellifera.
Kimura Y, Sakamura S, Ushijima T, Hama Y, Kajiura H, Fujiyama K, Okihara K, Hashimoto K, Sugimoto H, Yamada H
Biosci Biotechnol Biochem. Vol. 71 No. 4 p. 1111-1114-1114 2007年4月 研究論文(学術雑誌)
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Construction of Lactobacillus plantarum strain with enhanced L-lysine yield
M.N. Cahyanto, H. Kawasaki, M. Nagashio, K. Fujiyama, T. Seki
JOURNAL OF APPLIED MICROBIOLOGY Vol. 102 No. 3 p. 674-679 2007年2月 研究論文(学術雑誌)
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Production in plant of dengue 2 envelope domain III using TMV-based vector system
W. Saejung, K. Fujiyama, K. Hori, Y. Watanabe, T. Takasaki, I. Kurane, P. Malasit, T. Seki
Vaccine Vol. 25 p. 6646-6654 2007年1月 研究論文(学術雑誌)
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Replication-Competent Recombinant Vesicular Stomatitis Virus Encoding Hepatitis C Virus Envelope Proteins
H. Tani, Y. Komoda, E. Matsuo, K. Suzuki, I. Hamamoto, T. Yamashita, K. Moriishi, K. Fujiyama, T. Kanto, N. Hayashi, A. Owsianka, A. H. Patel, M. A. Whitt, Y.u Matsuura
J. Virology Vol. 81 p. 8601-8612 2007年1月 研究論文(学術雑誌)
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Production of mouse monoclonal antibody with galactose-extended sugar chain by suspension cultured tobacco BY2 cells expressing human b(1,4)-galactosyltransferase
K. Fujiyama, A. Furukawa, A. Katsura, R. Misaki, T. Omasa, T. Seki
Biochem. Biophys. Res. Commun. Vol. 358 No. 1 p. 85-91 2007年1月 研究論文(学術雑誌)
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In planta production of the immunogenic poliovirus peptide using TMV-based vector system.
K. Fujiyama, W. Saejung, I. Yanagihara, J. Nakado, R. Misaki, T. Honda, Y. Watanabe, T. Seki
J. Biosci. Bioeng Vol. 101 No. 5 p. 398-402 2006年11月 研究論文(学術雑誌)
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Production of Soluble Recombinant Dengue Virus 2 Envelope Domain III Protein in Escherichia coli
W. Saejung, C. Puttikhunt, T. Prommool, P. Sojikul, R. Tanaka, K. Fujiyama, P. Malasit, T. Seki
2006年10月
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Enhancement of recombinant soluble dengue virus 2 envelope domain III protein production Escherichia coli trxB and gor double mutant
Wanida Saejung, Chunya Puttikhunt, Tanapan Prommool, Punchapat Sojikul, Ryoji Tanaka, Kazuhito Fujiyama, Prida Malasit, Tatsuji Seki
JOURNAL OF BIOSCIENCE AND BIOENGINEERING Vol. 102 No. 4 p. 333-339 2006年10月 研究論文(学術雑誌)
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N-Linked glycan structures of a mouse monoclonal antibody produced from tobacco BY2 suspension-cultured cells
K. Fujiyama, R. Misaki, A. Katsura, T. Tanaka, A. Furukawa, T. Omasa, T. Seki
J. Biosci. Bioeng Vol. 101 No. 3 p. 212-218 2006年3月 研究論文(学術雑誌)
出版者・発行元:The Society for Biotechnology, Japan
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Isolation and characterization of nitrogen-fixing bacteria from soil and rhizosphere
Khiev P, 木谷茂, 木下浩, 川崎浩子, 藤山和仁, 仁平卓也, 関達治, Panbangred W
ユネスコバイオテクノロジー国際大学院研修講座第一期実施報告書 2006年3月 研究論文(大学,研究機関等紀要)
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Expression of human CMP-N-acetylneuraminic acid synthetase, and CMP-sialic acid transporter in tobacco suspension-cultured cell
R. Misaki, K. Fujiyama, T. Seki
Biochem. Biophys. Res. Commun Vol. 339 No. 4 p. 1184-1189 2006年1月 研究論文(学術雑誌)
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Process development and economic evaluation of recombinant human lactoferrin expressed in rice grain.
Nandi, S, Yalda, D, Lu, S, Nikolov, Z, Misaki, R, Fujiyama, K, Huang, N
Transgenic Res. Vol. 14 No. 3 p. 237-249 2005年6月 研究論文(学術雑誌)
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N-linked glycan structures of human lactoferrin produced by transgenic rice
K Fujiyama, Y Sakai, R Misaki, Yanagihara, I, T Honda, H Anzai, T Seki
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY Vol. 68 No. 12 p. 2565-2570 2004年12月 研究論文(学術雑誌)
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Characterization of Almond α-Mannosidase and Its Application for Structure Analysis of Sugar Chain
Ryo Misaki, Kazuhito Fujiyama, Hiroyuki Yokoyama, Yoshihiro Ido, Kiyotaka Miyauchi, Toshiomi Yoshida, Tatsuji Seki
Journal of Bioscience and Bioengineering Vol. 96 No. 2 p. 187-192 2003年2月 研究論文(学術雑誌)
出版者・発行元:The Society for Biotechnology, Japan
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In vivo conversion of a glycan to human compatible type by transformed tobacco cells
K Fujiyama, NQ Palacpac, H Sakai, Y Kimura, A Shinmyo, T Yoshida, T Seki
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS Vol. 289 No. 2 p. 553-557 2001年11月 研究論文(学術雑誌)
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Glycoproteins secreted from suspension-cultured tobacco BY2 cells have distinct glycan structures from intracellular glycoproteins
藤山 和仁
65/,2482-2488 Vol. 65 No. 11 p. 2482-2488 2001年11月 研究論文(学術雑誌)
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Human N-acetylglucosaminyltransferase I. Expression in Escherichia coli as a soluble enzyme, and application as an immobilized enzyme for the chemoenzymatic synthesis of N-linked oligosaccharides
藤山 和仁
92/,569-574 Vol. 92 No. 6 p. 569-574 2001年10月 研究論文(学術雑誌)
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Identification of Putative Gene Encoded on ORF 16 of the 81kb Contig of Arabidopsis thaliana Chromosome III as .ALPHA.-Mannosidase.
FUJIYAMA KAZUHITO, KIRA YUKO, IIZUKA MASAKAZU, KIMURA YOSHINOBU, SEKI TATSUJI
Journal of Bioscience and Bioengineering Vol. 92 No. 4 p. 401-404 2001年
出版者・発行元:The Society for Biotechnology, Japan
-
Characterization of Streptomyces D3 derived from protoplast fusion between Streptomyces cyaneus 190-1 and Streptomyces griseoruber 42-9
S Teeradakorn, Y Talawanich, Suzuki, I, P Pinphanichakarn, K Fujiyama, T Seki, T Yoshida
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY Vol. 14 No. 4 p. 565-570 1998年7月 研究論文(学術雑誌)
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A metabolic engineering view on molecular breeding of an alcohol fermenting yeast from xylose
T Seki, M Tantirungkij, K Fujiyama, T Yoshida
ENVIRONMENTAL BIOTECHNOLOGY p. 114-124 1996年 研究論文(国際会議プロシーディングス)
-
トリプシン処理したタテナタマメ由来α-マンノシダーゼについて(共著)
藤山和仁
68/3,244 1994年
出版者・発行元:日本農芸化学会誌
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Cloning of cDNAs encoding two peroxidases of ┣DBArabidopsis(/)-┫DB ┣DBthaliana(/)-┫DB and their organ-specific expression
藤山和仁
75/3,166-172 1993年 研究論文(学術雑誌)
出版者・発行元:Journal of Fermentation and Bioengineering
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Construction of Hybrid Genes of 6-Aminohexanoic Acid-Oligomer Hydrolase and Its Analogous Enzyme
藤山和仁
259 1984年 研究論文(学術雑誌)
出版者・発行元:Journal of Biological Chemistry