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Fujiyama Kazuhito

藤山 和仁

Professor

Education

  • - 1986/03, Osaka University, Graduate School, Division of Engineering
  • - 1986, Osaka University, Graduate School, Division of Engineering
  • - 1984/03, Osaka University, Faculty of Engineering
  • - 1984, Osaka University, Faculty of Engineering

Research History

  • 2009/02/01 - Present, Osaka University International Center for Biotechnology, Professor
  • 2003/02 - Present, 大阪大学助教授
  • 1988/04 - Present, Osaka University
  • 2007/04/01 - 2009/01/31, Osaka University International Center for Biotechnology, Associate Professor
  • 2003/02/01 - 2007/03/31, Osaka University International Center for Biotechnology, Associate Professor
  • 1995/04/01 - 2003/01/31, Osaka University International Center for Biotechnology, Research Assistant
  • 1988 - 2003, Osaka University, Research Assistant

Research Areas

  • Life sciences, Applied molecular and cellular biology
  • Manufacturing technology (mechanical, electrical/electronic, chemical engineering), Applied biofunctional and bioprocess engineering

Professional Memberships

  • 日本生化学会
  • 日本農芸化学会
  • 日本生物工学会

Papers

  • Bombyx mori β1,4-N-acetylgalactosaminyltransferase possesses relaxed donor substrate specificity in N-glycan synthesis, Hiroyuki Kajiura,Ryousuke Miyauchi,Akemi Kakudo,Takao Ohashi,Ryo Misaki,Kazuhito Fujiyama, Scientific Reports, Springer Science and Business Media LLC, Vol. 11, No. 1, 2021/12
  • Structure–function studies of ultrahigh molecular weight isoprenes provide key insights into their biosynthesis, Hiroyuki Kajiura,Takuya Yoshizawa,Yuji Tokumoto,Nobuaki Suzuki,Shinya Takeno,Kanokwan Jumtee Takeno,Takuya Yamashita,Shun-ichi Tanaka,Yoshinobu Kaneko,Kazuhito Fujiyama,Hiroyoshi Matsumura,Yoshihisa Nakazawa, Communications Biology, Springer Science and Business Media LLC, Vol. 4, No. 1, 2021/12
  • Improved Assay System for Acidic Peptide: N-glycanase (aPNGase) Activity in Plant Extracts, Chiharu YAMAMOTO,Mikako OGURA,Ryota UEMURA,Megumi MAEDA,Hiroyuki KAJIURA,Ryo MISAKI,Kazuhito FUJIYAMA,Yoshinobu KIMURA, Anal Biochem., 2021/09
  • Production of Human Acid-Alpha Glucosidase With a Paucimannose Structure by Glycoengineered Arabidopsis Cell Culture, Ratna Sariyatun,Florence,Hiroyuki Kajiura,Takao Ohashi,Ryo Misaki,Kazuhito Fujiyama, Frontiers in Plant Science, Frontiers Media SA, Vol. 12, 2021/07/14
  • Transient Production of Human β-Glucocerebrosidase With Mannosidic-Type N-Glycan Structure in Glycoengineered Nicotiana benthamiana Plants, Naphatsamon Uthailak,Hiroyuki Kajiura,Ryo Misaki,Kazuhito Fujiyama, Frontiers in Plant Science, Frontiers Media SA, Vol. 12, 2021/06/07
  • Direct evidence of cytosolic PNGase activity in Arabidopsis thaliana: in vitro assay system for plant cPNGase activity, Sahoko Shirai,Ryota Uemura,Megumi Maeda,Hiroyuki Kajiura,Ryo Misaki,Kazuhito Fujiyama,Yoshinobu Kimura, Bioscience, Biotechnology, and Biochemistry, Oxford University Press (OUP), Vol. 85, No. 6, p. 1460-1463, 2021/05/25
  • Structure and Biological Functions of Plant Glycans and Polysaccharides, Ryo Misaki,Kazuhito Fujiyama,Hiroyuki Kajiura, Comprehensive Glycoscience, Elsevier, p. 93-109, 2021
  • Functional characterization and overexpression of Δ12-desaturase in the oleaginous yeast Rhodotorula toruloides for production of linoleic acid-rich lipids, Chih Chan Wu,Takao Ohashi,Hiroyuki Kajiura,Yu Sato,Ryo Misaki,Kohsuke Honda,Savitree Limtong,Kazuhito Fujiyama, Journal of Bioscience and Bioengineering, 2021
  • Characterization of Bombyx mori N-acetylglucosaminyltransferase II splicing variants, Hiroyuki Kajiura,Yugo Nakamura,Mari Nishimura,Takao Ohashi,Ryo Misaki,Kazuhito Fujiyama, Biochemical and Biophysical Research Communications, Elsevier BV, Vol. 529, No. 2, p. 404-410, 2020/08
  • Enhancement of sialylation in rIgG in glyco-engineered Chinese hamster ovary cells, Nguyen TS,Misaki R,Ohashi T,Fujiyama K, Cytotechnology, Vol. 72, No. 3, p. 343-355, 2020/07
  • Ethanol and H2O2 stresses enhance lipid production in an oleaginous Rhodotorula toruloides thermotolerant mutant L1-1., Chih-Chan Wu,Takao Ohashi,Ryo Misaki,Savitree Limtong,Kazuhito Fujiyama, FEMS yeast research, Vol. 20, No. 4, 2020/06/01
  • Fully Human Monoclonal Antibodies Effectively Neutralizing Botulinum Neurotoxin Serotype B, Matsumura T,Amatsu S,Misaki R,Yutani M,Anariwa D,Kohda T,Fujiyama K,Ikuta K,Fujinaga Y, Toxins, Vol. 12, No. 5, 2020/05
  • Mating-type switching and mating-type gene array expression in the methylotrophic yeast Ogataea thermomethanolica TBRC656, Wongwisansri S,Promdonkoy P,Likhitrattanapisal S,Harnpichanchai P,Fujiyama K,Kaneko Y,Eurwilaichitr L,Ingsrisawang S,Tanapongpipat S, Microbiological Resarch, Vol. 232, 2020/02
  • Biosynthetic Pathway of Indole-3-Acetic Acid in Basidiomycetous Yeast Rhodosporidiobolus fluvialis, Bunsangiam S,Sakpuntoon V,Srisuk N,Ohashi T,Fujiyama K,Limtong S, Mycobiology, Vol. 47, No. 3, p. 292-300, 2019/07
  • Development of a Paper-Based Luminescence Bioassay for Therapeutic Monitoring of Aminoglycosides: a Proof-of-Concept Study., Matsuura H,Ujiie K,Duyen TTM,Izutsu K,Fujiyama K,Maeda S,Harada K,Hirata K, Applied Biochemistry and Biotechnology, 2019/05
  • The neutral N-linked glycans of the Basidiomycetous yeasts Pseudozyma antarctica and Malassezia furfur (Subphylum Ustilaginomycotina), Flores RJD,Ohashi T,Sakai K,Gonoi T,Kawasaki H,Fujiyama K, J Gen Appl Microbiol, Vol. 65, No. 2, p. 53-63, 2019/05
  • Delta-9 fatty acid desaturase overexpression enhanced lipid production and oleic acid content in Rhodosporidium toruloides for preferable yeast lipid production, Tsai YY,Ohashi T,Wu CC,Bataa D,Misaki R,Limtong S,Fujiyama K, J Biosci Bioeng, Vol. 127, No. 4, p. 430-440, 2019/04
  • Arabidopsis thaliana α1,2-L-fucosyltransferase catalyzes the transfer of L-galactose to xyloglucan oligosaccharide, Ohashi H,Ohashi T,Misaki R,Fujiyama K, FEBS Lett, 2019/01
  • Transcriptome sequencing and identification of cytochrome P450 monooxygenases involved in the biosynthesis of maslinic acid and corosolic acid in Avicennia marina., Mitsuki Nakamura,Tran My Linh,Le Quynh Lien,Hayato Suzuki,Nguyen Chi Mai,Vu Huong Giang,Keita Tamura,Nguyen Van Thanh,Hideyuki Suzuki,Ryo Misaki,Toshiya Muranaka,Ninh Khac Ban,Kazuhito Fujiyama,Hikaru Seki, Plant biotechnology (Tokyo, Japan), Vol. 35, No. 4, p. 341-348, 2018/12/25
  • St6gal1 knockdown alters HBV life cycle in HepAD38 cells, Surya Agung Priyambada,Ryo Misaki,Toru Okamoto,Takao Ohashi,Keiji Ueda,Yoshiharu Matsuura,Kazuhito Fujiyama, Biochemical and Biophysical Research Communications, Vol. 503, No. 3, p. 1841-1847, 2018/09
  • Isolation of a thermotolerant Rhodosporidium toruloides DMKU3-TK16 mutant and its fatty acid profile at high temperature, Wu CC,Tsai YY,Ohashi T,Misaki R,Limtong S,Fujiyama K, FEMS Microbiol Lett, 2018/08
  • The production of human β-glucocerebrosidase in Nicotiana benthamiana root culture, Naphatsamon U,Ohashi T,Misaki R,Fujiyama K, Int J Mol Sci, 2018/07
  • Enhancement of glycosylation by stable co-expression of two sialylation-related enzymes on Chinese hamster ovary cells, Nguyen TS,Misaki R,Ohashi T,Fujiyama K, J Biosci Bioeng, 2018/07
  • Hydroponic treatment of Nicotiana benthamiana with kifunensine modifies the N-glycans of recombinant glycoprotein antigens to predominantly Man9 high-mannose type upon transient overexpression., Roychowdhury S,Oh YJ,Kajiura H,Hamorsky KT,Fujiyama K,Matoba N, Front Plant Sci., Vol. 9, 2018/04
  • Molecular cloning and overexpression of DGA1, an acyl-CoA dependent diacylglycerol acyltransferase, in the oleaginous yeast Rhodosporidiobolus fluvialis DMKU-RK253, Polburee P,Ohashi T,Tsai YY,Sumyai T,Lertwattanasakul N,Limtong S,Fujiyama K, Microbiology, 2018/01
  • Isolation and characterization of a stress-responsive gene encoding a CHRD domain-containing protein from a halotolerant green alga, Ryo Ishinishi,Hideyuki Matsuura,Satoshi Tanaka,Saaya Nozawa,Keisuke Tanada,Norihito Kawashita,Kazuhito Fujiyama,Hitoshi Miyasaka,Kazumasa Hirata, GENE, ELSEVIER SCIENCE BV, Vol. 640, p. 14-20, 2018/01
  • Effective Synthesis of Guanosine 5’-Diphospho-β-L-galactose Using Bacterial L-Fucokinase/Guanosine 5’-diphosphate-L-fucose Pyrophosphorylase, Ohashi H,Wahl C,Ohashi T,Elling L,Fujiyama K, Adv Synth Catal, 2017/10
  • Cell surface N-glycan alteration in HepAD38 cell lines expressing Hepatitis B virus, Surya Agung Priyambada,Ryo Misaki,Toru Okamoto,Yuta Okamoto,Takao Ohashi,Keiji Ueda,Yoshiharu Matsuura,Kazuhito Fujiyama, Virus Research, Vol. 238, p. 101-109, 2017/06/15
  • Biochemical characterization of rhamnosyltransferase involved in biosynthesis of pectic rhamnogalacturonan I in plant cell wall, Uehara Y,Tamura S,Maki Y,Yagyu K,Mizoguchi T,Tamiaki H,Imai T,Ishii T,Ohashi T,Fujiyama K,Ishimizu T, Biochem Biophys Res Commun, 2017/04
  • The neutral N-linked glycans of the Ustilaginomycetes yeast Sympodiomycopsis pahiopedili, Flores RJD,Ohashi T,Kawasaki H,Fujiyama K, Yeast, 2017/04
  • Two Eucommia farnesyl diphosphate synthases exhibit distinct enzymatic properties leading to end product preferences., Kajiura H,Suzuki N,Tokumoto Y,Yoshizawa T,Takeno S,Fujiyama K,Kaneko Y,Matsumura H,Nakazawa Y, Biochimie, ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER, Vol. 139, p. 95-106-106, 2017/04
  • N-glycan structures and downstream mannose-phosphorylation of plant recombinant human alpha-L-iduronidase: toward development of enzyme replacement therapy for mucopolysaccharidosis I., Pierce OM,McNair GR,He X,Kajiura H,Fujiyama K,Kermode AR, Plant Mol Biol., SPRINGER, Vol. 95, No. 6, p. 593-606-606, 2017/04
  • A polygalacturonase localized in the Golgi apparatus in Pisum sativum, Ohashi T,Jinno J,Inoue Y,Ito S,Fujiyama K,Ishimizu T, J Biochem, 2017/02
  • Fucosyltransferases produce N-glycans containing core L-galactose, Ohashi H,Ohashi T,Kajiura H,Misaki R,Kitamura S,Fujiyama K, Biochem Biophys Res Commun, 2017/01
  • Lipid production from biodiesel-derived crude glycerol by Rhodosporidium fluviale DMKU-RK253 using temperature shift with high cell density, Pirapan Polburee,Wichien Yongmanitchai,Kohsuke Honda,Takao Ohashi,Toshiomi Yoshida,Kazuhito Fujiyama,Savitree Limtong, BIOCHEMICAL ENGINEERING JOURNAL, ELSEVIER SCIENCE BV, Vol. 112, p. 208-218, 2016/08
  • Development of a sufficient and effective procedure for transformation of an oleaginous yeast, Rhodosporidium toruloides DMKU3‑TK16, Tsai Y-Y,Ohashi T,Kanazawa T,Polburee P,Misaki R,Limtong S,Fujiyama K, Curr Genet, 2016/07
  • Core-fucosylation plays a pivotal role in hepatitis B pseudo virus infection: a possible implication for HBV glycotherapy, Shinji Takamatsu,Mayuka Shimomura,Yoshihiro Kamada,Haruka Maeda,Tomoaki Sobajima,Hayato Hikita,Masumi Iijima,Yuta Okamoto,Ryo Misaki,Kazuhito Fujiyama,Shushi Nagamori,Yoshikatsu Kanai,Tetsuo Takehara,Keiji Ueda,Shun'ichi Kuroda,Eiji Miyoshi, Glycobiology, Oxford University Press (OUP), 2016/06/21
  • Altered glycosylation of exported proteins,including surface immune receptors, compromises calcium and downstream signaling responses to microbe-associated molecular patterns in Arabidopsis thaliana., Trempel F,Kajiura H,Ranf S,Grimmer J,Westphal L,Zipfel C,Scheel D,Fujiyama K,Lee J, BMC Plant Biol., Vol. 16, 2016/04
  • Recombinant production and characterization of human anti-influenza virus monoclonal antibodies identified from hybridomas fused with human lymphocytes., Misaki R,Fukura N,Kajiura H,Yasugi M,Kubota-Koketsu R,Sasaki T,Momota M,Ono K,Ohashi T,Ikuta K,Fujiyama K, Biologicals, Vol. 44, 2016/04
  • A novel potential signal peptide sequence and overexpression of ER-resident chaperones enhance heterologous protein secretion in thermotolerant methylotrophic yeast Ogataea thermomethanolica, Roongsawang N,Puseenam A,Kitikhun S,Sae-Tang K,Harnpicharnchai P,Ohashi T,Fujiyama K,Tirasophon W,Tanapongpipat S, Appl Biochem Biotechnol, 2015/10
  • Substrate preference of citrus naringenin rhamnosyltransferases and their application to flavonoid glycoside production in fission yeast, Takao Ohashi,Yuka Hasegawa,Ryo Misaki,Kazuhito Fujiyama, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER, Vol. 100, No. 2, p. 687-696, 2015/10
  • Characterization of oleaginous yeasts accumulating high levels of lipid when cultivated in glycerol and their potential for lipid production from biodiesel-derived crude glycerol, Polburee P,Yongmanitchai W,Lertwattanasakul N,Ohashi T,Fujiyama K,Limtong S, Fungal Biol, 2015/09
  • N-glycosylation of cholera toxin B subunit in Nicotiana benthamiana: impacts on host stress response, production yield and vaccine potential., Hamorsky KT,Kouokam JC,Jurkiewicz JM,Nelson B,Moore LJ,Husk AS,Kajiura H,Fujiyama K,Matoba N, Sci Rep., Vol. 23, 2015/04
  • Im"plant"ing of mammalian glycosyltransferase gene into plant suspension-cultured cells using Agrobacterium-mediated transformation., Kajiura H,Fujiyama K, Methods Mol Biol., Springer New York, Vol. 1321, p. 225-232-232, 2015/04
  • Production of initial-stage eukaryotic N-glycan and its protein glycosylation in Escherichia coli, Akkaraphol Srichaisupakit,Takao Ohashi,Ryo Misaki,Kazuhito Fujiyama, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, SOC BIOSCIENCE BIOENGINEERING JAPAN, Vol. 119, No. 4, p. 399-405, 2015/04
  • Sialylation potentials of the silkworm, Bombyx mori; B.mori possesses an active α2,6-sialyltransferase., Kajiura H,Hamaguchi Y,Mizushima H,Misaki R,Fujiyama K, Glycobiology, OXFORD UNIV PRESS INC, Vol. 25, No. 12, p. 1441-1453-1453, 2015/04
  • Barnettozyma siamensis f.a., sp. nov., a lipid-accumulating ascomycete yeast species, Polburee P,Yongmanitchai W,Ohashi T,Fujiyama K,Limtong S, Int J Syst Evol Microbiol, Vol. 64, No. Pt 9, p. 3053-3057, 2014/09
  • Identification of a protein glycosylation operon from Campylobacter jejuni JCM 2013 and its heterologous expression in Escherichia coli, Srichaisupakit A,Ohashi T,Fujiyama K, J Biosci Bioeng, Vol. 118, No. 3, p. 256-262, 2014/09
  • Antibody germline characterization of cross-neutralizing human IgGs against 4 serotypes of dengue virus., Pannamthip Pitaksajjakul,Surachet Benjathummarak,Cholnatip Pipattanaboon,Waranya Wongwit,Tamaki Okabayashi,Motoki Kuhara,Ryo Misaki,Kazuhito Fujiyama,Pongrama Ramasoota, Biochemical and Biophysical Research Communicatioins, Vol. 446, No. 2, p. 475-480, 2014/04
  • Cloning of a cDNA encoding the Gly m Bd 28K precursor and its vacuole transport in tobacco BY2 suspension-cultured cells., Hitomi Yumioka-Ito,Ryo Misaki,Miyuki Yokoro,Makiko Suzuki,Hiromi Yamashita,Miki Hiemori-Kondo,Masumi Kimoto,Ko Kato,Kazuhito Fujiyama,Hideaki Tsuji, Journal of nutritional science and vitaminology, Vol. 60, No. 2, p. 129-39, 2014
  • Emerging Antigenic Variants at the Antigenic Site Sb in Pandemic A(H1N1)2009 Influenza Virus in Japan Detected by a Human Monoclonal Antibody, M. Yasugi,R. Kubota-Koketsu,A. Yamashita,N. Kawashita,A. Du,R. Misaki,M. Kuhara,N. Boonsathorn,K. Fujiyama,Y. Okuno,T. Nakaya,K. Ikuta, PLoS One, Vol. 8, No. 10, 2013/10
  • Dengue virus neutralization and antibody-ependetn enhancement activities of human monoclonal antibodies derived from dengue patiensts as acute phase of secondary infections., Tadahiro Sasaki,Chayanee Setthapramote,Takeshi Kurosu,Mituhiro Nishimura,Azusa Asai,Tamaki Okabayashi,Magot Omokoko,Chonlatip Pipattanaboon,Pannnamthip Pitaksajjakul,Kriengsak, Limittikul,Arunee Subchareon,Panjaporn Chaichana,Tamaki Okabayashi,Itaru Hirai,Pornsawan Leaungwutiwong,Ryo Misaki,Kazuhiro Fujiyama,Ken-ichiro Ono,Yoshinobu Okuno,Pongrama Ramasoota,Kazuyoshi Ikuta, Antiviral Research, 2013/06
  • Rapid and scalable plant-based production of a cholera toxin B subunit variant to aid in mass vaccination against cholera outbreaks., Hamorsky KT,Kouokam JC,Bennett LJ,Baldauf KJ,Kajiura H,Fujiyama K,Matoba N, PLoS Negl Trop Dis., Vol. 7, 2013/04
  • Transgenic Rice Seeds Accumulating Recombinant Hypoallergenic Birch Pollen Allergen Bet v 1 Generate Giant Protein Bodies., Wang S,Takahashi H,Kajiura H,Kawakatsu T,Fujiyama K,Takaiwa F, Plant Cell Physiol., Vol. 54, 2013/04
  • N-Glycosylation and N-Glycan moieties of CTB expressed in rice seeds., Kajiura H,Wasai M,Kasahara S,Takaiwa F,Fujiyama K, Mol Biotechnol., HUMANA PRESS INC, Vol. 54, No. 3, p. 784-794-794, 2013/04
  • Human monoclonal antibodies broadly neutralizing against influenza B virus., Yasugi M,Kubota-Koketsu R,Yamashita A,Kawashita N,Du A,Sasaki T,Nishimura M,Misaki R,Kuhara M,Boonsathorn N,Fujiyama K,Okuno Y,Nakaya T,Ikuta K, PLoS Pathog, 2013/02
  • Characterization of URA3 gene in oleaginous yeast Rhodosporidium toruloides DMKU3-TK16 for lipid engineering, Takenori Kanazawa,Takao Ohashi,Ayako Tomio,Ryo Misaki,Takeshi Bamba,Eiichiro Fukusaki,Limtong Savitree,Kazuhito Fujiyama, 2012/10
  • Production of different glycosylation variants of the tumour-targeting mAb H10 in Nicotiana benthamiana: influence on expression yield and antibody degradation., Lombardi R,Donini M,Villani ME,Brunetti P,Fujiyama K,Kajiura H,Paul M,Ma JK,Benvenuto E, Transgenic Res., Vol. 21, 2012/04
  • Evolutionarily conserved glycan signal to degrade aberrant brassinosteroid receptors in Arabidopsis., Hong Z,Kajiura H,Su W,Jin H,Kimura A,Fujiyama K,Li J, Proc Natl Acad Sci U S A, Vol. 109, 2012/04
  • N-Glycan structures of glycoproteins in suspension-cultured Arabidopsis thaliana T87 cells., Kajiura H,Tanaka R,Kato K,Hamaguchi Y,Seki T,Fujiyama K, Plant Biotechnol., JAPANESE SOC PLANT CELL & MOLECULAR BIOLOGY, Vol. 29, No. 5, p. 489-493-493, 2012/04
  • Enhancement of sialylation on humanized IgG-like bispecific antibody by overexpression of alpha2,6-sialyltransferase derived from Chinese hamster ovary cells., Onitsuka M,Kim WD,Ozaki H,Kawaguchi A,Honda K,Kajiura H,Fujiyama K,Asano R,Kumagai I,Ohtake H,Omasa T, Appl Microbiol Biotechnol., SPRINGER, Vol. 94, No. 1, p. 69-80-80, 2012/04
  • Arabidopsis beta 1,2-xylosyltransferase: Substrate specificity and participation in the plant-specific N-glycosylation pathway, Hiroyuki Kajiura,Toru Okamoto,Ryo Misaki,Yoshiharu Matsuura,Kazuhito Fujiyama, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, SOC BIOSCIENCE BIOENGINEERING JAPAN, Vol. 113, No. 1, p. 48-54, 2012/01
  • N-terminal vacuolar sorting signal at the mouse antibody alters the N-linked glycosylation pattern in suspension-cultured tobacco BY2 cells, Ryo Misaki,Yohei Sakai,Takeshi Omasa,Kazuhito Fujiyama,Tatsuji Seki, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, SOC BIOSCIENCE BIOENGINEERING JAPAN, Vol. 112, No. 5, p. 476-484, 2011/11
  • Improved expression and characterization of recombinant human Golgi alpha 1, 2-mannosidase I isoforms (IA2 and IC) by Escherichia coli, Junko Isoyama-Tanaka,Koji Dohi,Ryo Misaki,Kazuhito Fujiyama, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, SOC BIOSCIENCE BIOENGINEERING JAPAN, Vol. 112, No. 1, p. 14-19, 2011/07
  • Reduced Immunogenicity of Arabidopsis hgl1 Mutant N-Glycans Caused by Altered Accessibility of Xylose and core Fucose Epitopes, Heidi Kaulfuerst-Soboll,Stephan Rips,Hisashi Koiwa,Hiroyuki Kajiura,Kazuhito Fujiyama,Antje von Schaewen, JOURNAL OF BIOLOGICAL CHEMISTRY, AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, Vol. 286, No. 26, p. 22955-22964, 2011/07
  • Double-Knockout of Putative Endo-beta-N-acetylglucosaminidase (ENGase) Genes in Arabidopsis thaliana: Loss of ENGase Activity Induced Accumulation of High-Mannose Type Free N-Glycans Bearing N,N '-Acetylchitobiosyl Unit, Yoshinobu Kimura,Yuki Takeoka,Masami Inoue,Megumi Maeda,Kazuhito Fujiyama, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, TAYLOR & FRANCIS LTD, Vol. 75, No. 5, p. 1019-1021, 2011/05
  • Reduced immunogenicity of Arabidopsis hybrid glycosylation1 (hgl1) N-glycans due to altered accessibility of xylose and core fucose epitopes., Kaulfuerst-Soboll H,Rips S,Koiwa H,Kajiura H,Fujiyama K,von Schaewen A, J Biol Chem., Vol. 286, 2011/04
  • Stable coexpression of two human sialylation enzymes in plant suspension-cultured tobacco cells., Kajiura H,Misaki R,Fujiyama K,Seki T, J Biosci Bioeng., SOC BIOSCIENCE BIOENGINEERING JAPAN, Vol. 111, No. 4, p. 471-477-477, 2011/04
  • Jack bean alpha-mannosidase digestion profile of hybrid-type N-glycans: Effect of reaction pH on substrate preference, Koji Dohi,Junko Isoyama-Tanaka,Ryo Misaki,Kazuhito Fujiyama, BIOCHIMIE, ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER, Vol. 93, No. 4, p. 766-771, 2011/04
  • Rice Golgi proteome : identification and characterization of N-acetylglucosaminyltransferase-I-like protein., Mitsui T,Asakura T,Nakayama Y,Takayama K,Hirose S,Katamine H,Aoyama M,Karahashi A,Kaneko K,Ishiyama R,Itoh K,Hori H,Kajiura H,Fujiyama K, Bull. Facul. Agric. Niigata Univ., Vol. 63, 2010/04
  • The impact of the overexpression of human UDP-galactose transporter gene hUGT1 in tobacco plants., Khalil MF,Kajiura H,Fujiyama K,Koike K,Ishida N,Tanaka N, J Biosci Bioeng., Vol. 109, 2010/04
  • Arabidopsis thaliana ALG3 mutant synthesizes immature oligosaccharides in the ER and accumulates unique N-glycans., Kajiura H,Seki T,Fujiyama K, Glycobiology, OXFORD UNIV PRESS INC, Vol. 20, No. 6, p. 736-751-751, 2010/04
  • Two Arabidopsis thaliana Golgi alpha-mannosidase I enzymes are responsible for plant N-glycan maturation, Hiroyuki Kajiura,Hisashi Koiwa,Yoshihisa Nakazawa,Atsushi Okazawa,Akio Kobayashi,Tatsuji Seki,Kazuhito Fujiyama, GLYCOBIOLOGY, OXFORD UNIV PRESS INC, Vol. 20, No. 2, p. 235-247, 2010/02
  • Biochemical and immunological characterization of the plant-derived candidate human immunodeficiency virus type 1 mucosal vaccine CTB-MPR., Matoba N,Kajiura H,Cherni I,Doran JD,Bomsel M,Fujiyama K,Mor TS, Plant Biotechnol J., Vol. 7, 2009/04
  • Cloning and characterization of cytidine monophosphate-3-deoxy-D-manno-octulosonate synthetase from Arabidopsis thaliana., Misaki R,Kajiura H,Fujii K,Fujiyama K,Seki T, J Biosci Bioeng., SOC BIOSCIENCE BIOENGINEERING JAPAN, Vol. 108, No. 6, p. 527-529-529, 2009/04
  • Molecular Cloning and Gene Expression Analysis of Tomato Endo-beta-N-acetylglucosaminidase, an Endoglycosidase Involved in the Production of High-Mannose Type Free N-Glycans during Tomato Fruit Ripening, Kosuke Nakamura,Masami Inoue,Megumi Maeda,Ryohei Nakano,Katsutoshi Hosoi,Kazuhito Fujiyama,Yoshinobu Kimura, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, TAYLOR & FRANCIS LTD, Vol. 73, No. 2, p. 461-464, 2009/02
  • Change in glycosylation pattern with extension of endoplasmic reticulum retention signal sequence of mouse antibody produced by suspension-cultured tobacco BY2 cells, Kazuhito Fujiyama,Ryo Misaki,Yohei Sakai,Takeshi Omasa,Tatsuji Seki, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, SOC BIOSCIENCE BIOENGINEERING JAPAN, Vol. 107, No. 2, p. 165-172, 2009/02
  • Analysis of CMP-sialic acid transporter-like proteins in plants, Shou Takashima,Junichi Seino,Takeshi Nakano,Kazuhito Fujiyama,MasafumiTsujimoto,Nobuhiro Ishida,Yasuhiro Hashimoto, Phytochemistry, 2009/01
  • Generation of a transgenic rice seed-based edible vaccine against house dust mite allergy., Yang L,Kajiura H,Suzuki K,Hirose S,Fujiyama K,Takaiwa F, Biochem Biophys Res Commun., Vol. 365, 2008/04
  • Salt tolerance of Arabidopsis thaliana requires maturation of N-glycosylated proteins in the Golgi apparatus., Kang JS,Frank J,Kang CH,Kajiura H,Vikram M,Ueda A,Kim S,Bahk JD,Triplett B,Fujiyama K,Lee SY,von,Schaewen A,Koiwa H, Proc Natl Acad Sci U S A, Vol. 105, 2008/04
  • Salt tolerance of Arabidopsis thaliana requires maturation of N-glycosylated proteins in the Golgi, J. S. Kang,J. Frank,C. H. Kang,H. Kajiura,M. Vikram,A. Ueda,S. Kim,J. D. Bahk,B. Triplett,K. Fujiyama,S. Y. Lee,A. von Schaewen,H.i Koiwa, Proc Natl Acad Sci USA., 2008/04
  • Biochemical and immunological characterization of the plant-derived candidate human immunodeficiency virus type 1 mucosal vaccine CTB-MPR649-684, Nobuyuki Matoba,Hiroyuki Kajiura,Irene Cherni,Jeffrey D Doran,Morgane Bomsel,Kazuhito Fujiyama,Tsafrir S Mor, Plant Biotechnol J, 2008/02
  • Salt-adaptation of Tobacco BY2 Cells Induces Change in Glycoform of N-Glycans: Enhancement of Exo- and Endo-glycosidase Activities by Salt-adaptation., Y. Kimura,T. Watanabe,M. Maeda,Y. Murata,K. Fujiyama, Biosci. Biotechnol. Biochem., Vol. 72, No. 2, p. 514-522, 2008/02
  • Generation of a transgenic rice seed-based edible vaccine against house dust mite allergy, L. Yang,H. Kajiura,K. Suzuki,S. Hirose,K. Fujiyama,F. Takaiwa, Biochem. Biophys. Res. Commun., 2007/10
  • Molecular Cloning and Expression of Two Novel -N-acetylglucosaminidases from Silkworm Bombyx mori, T. Okada,S. Ishiyama,H. Sezutsu,A. Usami,T. Tamura,K. Mita,K. Fujiyama,T. Seki, Biosci. Biotechnol. Biochem., TAYLOR & FRANCIS LTD, Vol. 71, No. 7, p. 1626-1635, 2007/07
  • First Evidence for 1-3 Galactosyltransferase Activity Involved in Biosynthesis of Insect Complex Type N-Glycan Harboring T-Antigen in Apis mellifera, Y. Kimura,S. Sakamura,T. Ushijima,Y. Hama,H. Kajiura,K. Fujiyama,K. Okihara,K. Hashimoto,H. Sugimoto,H. Yamada, Biosci. Biotechnol. Biochem., 2007/04
  • Evidence for new beta1-3 galactosyltransferase activity involved in biosynthesis of unusual N-glycan harboring T-antigen in Apis mellifera., Kimura Y,Sakamura S,Ushijima T,Hama Y,Kajiura H,Fujiyama K,Okihara K,Hashimoto K,Sugimoto H,Yamada H, Biosci Biotechnol Biochem., TAYLOR & FRANCIS LTD, Vol. 71, No. 4, p. 1111-1114-1114, 2007/04
  • Construction of Lactobacillus plantarum strain with enhanced L-lysine yield, M. N. Cahyanto,H. Kawasaki,M. Nagashio,K. Fujiyama,T. Seki, JOURNAL OF APPLIED MICROBIOLOGY, WILEY-BLACKWELL, Vol. 102, No. 3, p. 674-679, 2007/02
  • Production in plant of dengue 2 envelope domain III using TMV-based vector system, W. Saejung,K. Fujiyama,K. Hori,Y. Watanabe,T. Takasaki,I. Kurane,P. Malasit,T. Seki, Vaccine, 2007/01
  • Replication-Competent Recombinant Vesicular Stomatitis Virus Encoding Hepatitis C Virus Envelope Proteins, H. Tani,Y. Komoda,E. Matsuo,K. Suzuki,I. Hamamoto,T. Yamashita,K. Moriishi,K. Fujiyama,T. Kanto,N. Hayashi,A. Owsianka,A. H. Patel,M. A. Whitt,Y.u Matsuura, J. Virology, 2007/01
  • Production of mouse monoclonal antibody with galactose-extended sugar chain by suspension cultured tobacco BY2 cells expressing human b(1,4)-galactosyltransferase, K. Fujiyama,A. Furukawa,A. Katsura,R. Misaki,T. Omasa,T. Seki, Biochem. Biophys. Res. Commun., ACADEMIC PRESS INC ELSEVIER SCIENCE, Vol. 358, No. 1, p. 85-91, 2007/01
  • In planta production of immunogenic poliovirus peptide using tobacco mosaic virus-based vector system, Kazuhito Fujiyama,Wanida Saejung,Itaru Yanagihara,Junko Nakado,Ryo Misaki,Takeshi Honda,Yuichiro Watanabe,Tatsuji Seki, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, SOC BIOSCIENCE BIOENGINEERING JAPAN, Vol. 101, No. 5, p. 398-402, 2006/11
  • Production of Soluble Recombinant Dengue Virus 2 Envelope Domain III Protein in Escherichia coli, W. Saejung,C. Puttikhunt,T. Prommool,P. Sojikul,R. Tanaka,K. Fujiyama,P. Malasit,T. Seki, 2006/10
  • Enhancement of recombinant soluble dengue virus 2 envelope domain III protein production Escherichia coli trxB and gor double mutant, Wanida Saejung,Chunya Puttikhunt,Tanapan Prommool,Punchapat Sojikul,Ryoji Tanaka,Kazuhito Fujiyama,Prida Malasit,Tatsuji Seki, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, SOC BIOSCIENCE BIOENGINEERING JAPAN, Vol. 102, No. 4, p. 333-339, 2006/10
  • N-Linked glycan structures of a mouse monoclonal antibody produced from tobacco BY2 suspension-cultured cells, K. Fujiyama,R. Misaki,A. Katsura,T. Tanaka,A. Furukawa,T. Omasa,T. Seki, J. Biosci. Bioeng, 2006/03
  • Isolation and characterization of nitrogen-fixing bacteria from soil and rhizosphere, Khiev P,木谷茂,木下浩,川崎浩子,藤山和仁,仁平卓也,関達治,Panbangred W, ユネスコバイオテクノロジー国際大学院研修講座第一期実施報告書, 2006/03
  • Expression of human CMP-N-acetylneuraminic acid synthetase and CMP-sialic acid transporter in tobacco suspension-cultured cell, R Misaki,K Fujiyama,T Seki, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC ELSEVIER SCIENCE, Vol. 339, No. 4, p. 1184-1189, 2006/01
  • N-linked glycan structures of human lactoferrin produced by transgenic rice, K Fujiyama,Y Sakai,R Misaki,Yanagihara, I,T Honda,H Anzai,T Seki, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, TAYLOR & FRANCIS LTD, Vol. 68, No. 12, p. 2565-2570, 2004/12
  • Characterization of Almond α-Mannosidase and Its Application for Structure Analysis of Sugar Chain, Ryo Misaki,Kazuhito Fujiyama,Hiroyuki Yokoyama,Yoshihiro Ido,Kiyotaka Miyauchi,Toshiomi Yoshida,Tatsuji Seki, Journal of Bioscience and Bioengineering, 2003/02
  • In vivo conversion of a glycan to human compatible type by transformed tobacco cells, K Fujiyama,NQ Palacpac,H Sakai,Y Kimura,A Shinmyo,T Yoshida,T Seki, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC, Vol. 289, No. 2, p. 553-557, 2001/11
  • Glycoproteins secreted from suspension-cultured tobacco BY2 cells have distinct glycan structures from intracellular glycoproteins, FUJIYAMA Kazuhito, 65/,2482-2488, Biosci. Biotechnol. Biochem., Vol. 65, No. 11, p. 2482-2488, 2001/11
  • Human N-acetylglucosaminyltransferase I. Expression in Escherichia coli as a soluble enzyme, and application as an immobilized enzyme for the chemoenzymatic synthesis of N-linked oligosaccharides, FUJIYAMA Kazuhito, 92/,569-574, J. Biosci. Bioeng., Vol. 92, No. 6, p. 569-574, 2001/10
  • Identification of Putative Gene Encoded on ORF 16 of the 81kb Contig of Arabidopsis thaliana Chromosome III as .ALPHA.-Mannosidase., FUJIYAMA KAZUHITO,KIRA YUKO,IIZUKA MASAKAZU,KIMURA YOSHINOBU,SEKI TATSUJI, Journal of Fermentation Technology, The Society for Biotechnology, Japan, Vol. 92, No. 4, p. 401-404, 2001
  • Characterization of Streptomyces D3 derived from protoplast fusion between Streptomyces cyaneus 190-1 and Streptomyces griseoruber 42-9, S Teeradakorn,Y Talawanich,Suzuki, I,P Pinphanichakarn,K Fujiyama,T Seki,T Yoshida, WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, KLUWER ACADEMIC PUBL, Vol. 14, No. 4, p. 565-570, 1998/07
  • A metabolic engineering view on molecular breeding of an alcohol fermenting yeast from xylose, T Seki,M Tantirungkij,K Fujiyama,T Yoshida, ENVIRONMENTAL BIOTECHNOLOGY, KLUWER ACADEMIC PUBL, p. 114-124, 1996
  • Characterization of tryptic jack bean α-mannosidase, FUJIYAMA Kazuhito, 日本農芸化学会誌, 1994
  • Cloning of cDNAs encoding two peroxidases of ┣DBArabidopsis(/)-┫DB ┣DBthaliana(/)-┫DB and their organ-specific expression, FUJIYAMA Kazuhito, Journal of Fermentation and Bioengineering, 1993
  • Construction of Hybrid Genes of 6-Aminohexanoic Acid-Oligomer Hydrolase and Its Analogous Enzyme, FUJIYAMA Kazuhito, Journal of Biological Chemistry, 1984

Misc.

  • Improvement of glycosylation structure by suppression of β-N-acetylglucosaminidases in silkworm, Nomura Tsuyoshi,Suganuma Masatoshi,Higa Yukiko,Kataoka Yukiko,Funaguma Shunsuke,Okazaki Hironobu,Suzuki Takeo,Kobayashi Isao,Sezutsu Hideki,Fujiyama Kazuhito, Journal of bioscience and bioengineering, The Society for Biotechnology, Japan, Vol. 119, No. 2, p. 131-136, 2015/02
  • 2P-256 Modification of plant glycosylation with glycosyltransferases fused to immunoglobulin-constant regions, Hadae Yukihiro,Misaki Ryo,Ohashi Takao,Fujiyama Kazuhito, Vol. 66, p. 170-170, 2014
  • 3S-Ea04 Pharmaceutical protein production using heterologous host systems, Fujiyama Kazuhito, Vol. 66, p. 191-191, 2014
  • 3P-240 Development of UDP-Rha production system using fission yeast, Hasegawa Yuka,Ohashi Takao,Fujiyama Kazuhito, Vol. 65, p. 248-248, 2013
  • 3Ha11 Application of different 5' untranslated sequences for higher expression of human β-glucocerebrosidase in tobacco BY2 suspension culture :, Hong Nhung Ly,Kato Ko,Misaki Ryo,Kajiura Hiroyuki,Sasami Takahiro,Fujiyama Kazuhito, Vol. 64, p. 150-150, 2012
  • 3Ha09 Construction and activity assay of chimeric glycosyltransferases fused to immunoglobulin-constant regions., Hadae Yukihiro,Misaki Ryou,Fujiyama Kazuhito, Vol. 64, p. 150-150, 2012
  • 3Ha08 Structural analysis of glycans produced in Schizosaccharomyces pombe α1,3-galactosyltransferase disruptants, Ohashi Hiroyuki,Ohashi Takao,Fujiyama Kazuhito, Vol. 64, p. 150-150, 2012
  • 4Cp15 Identification of NeuAc synthetase from Clostridium kluyveri, Miyauchi Ryosuke,Kajiura Hiroyuki,Fujiyama Kazuhito, Vol. 64, p. 198-198, 2012
  • Glycosylation pattern of humanized IgG-like bispecific antibody produced by recombinant CHO cells., Kim WD,Tokunaga M,Ozaki H,Ishibashi T,Honda K,Kajiura H,Fujiyama K,Asano R,Kumagai I,Omasa T,Ohtake H, Appl Microbiol Biotechnol., SPRINGER, Vol. 85, No. 3, p. 535-542-542, 2010/04
  • Glycosylation pattern of humanized IgG-like bispecific antibody produced by recombinant CHO cells, Wook-Dong Kim,Miwako Tokunaga,Hiroyuki Ozaki,Takuya Ishibashi,Kohsuke Honda,Hiroyuki Kajiura,Kazuhito Fujiyama,Ryutaro Asano,Izumi Kumagai,Takeshi Omasa,Hisao Ohtake, Applied Microbiology and Biotechnology, SPRINGER, Vol. 85, No. 3, p. 535-542, 2010/01
  • N-linked glycan structures of glycoproteins in suspension-cultured Arabidopsis thaliana MM2d cells, Kazuhito Fujiyama,Ryoji Tanaka,Masaaki Terashima,Tatsuji Seki, PLANT BIOTECHNOLOGY, JAPANESE SOC PLANT CELL & MOLECULAR BIOLOGY, Vol. 24, No. 2, p. 255-259, 2007/01
  • Cloning of Lactobacillus plantarum IAM 12477 lysine biosynthetic genes encoding functional aspartate semialdehyde dehydrogenase, dihydrodipicolinate synthase, and dihydrodipicolinate reductase, MN Cahyanto,H Kawasaki,K Fujiyama,T Seki, WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, SPRINGER, Vol. 22, No. 4, p. 409-416, 2006/01
  • Regulation of aspartokinase, aspartate semialdehyde dehydrogenase, dihydrodipicolinate synthase and dihydrodipicolinate reductase in Lactobacillus plantarum, MN Cahyanto,H Kawasaki,M Nagashio,K Fujiyama,T Seki, MICROBIOLOGY-SGM, SOC GENERAL MICROBIOLOGY, Vol. 152, p. 105-112, 2006/01
  • 組換え医療用糖タンパク質の生産に向けた宿主細胞の育種, 酵素工学ニュース(酵素工学研究会), 2006
  • Glycotechnology in plant for molecular farming, FUJIYAMA Kazuhito, Bioscience & industry, Vol. 62, No. 12, p. 799-804, 2004/12/01
  • Plant cultured cells expressing human beta1,4-galactosyltransferase secrete glycoproteins with galactose-extended N-linked glycans, FUJIYAMA Kazuhito, 13/,199-205, OXFORD UNIV PRESS INC, Vol. 13, No. 3, p. 199-205, 2003/03
  • Miti, M. Kazuhito Fujiyama, C. Robert Flynn, Lokesh Joshi : Sialylated endogenous giycoproteins in suspension cultured cells of <i>Arabidopsis thaliana</i>, Nature Biotech., Vol. , 21, 1470-1471/,, 2003
  • Ryo Misaki, Hidekazu Nagaya, Kazuhito Fujiyama, Itaru Yanagihara, Takeshi Honda, and Tatsuji Seki : <i>N</i>-Linked Glycan Structures of Mouse Interferon<sup>-</sup>â Produced by <i>Bombyx mori Larvae</i>, Biochem. Biophys. Res. Commun., Vol. 311(4), 979-986/,, 2003
  • Effect of alpha 1,2-mannosidic linkage located in alpha 1,3-branch of Man6GlcNAc2 oligosaccharide on enzyme activity of recombinant human Man(9)-mannosidase produced in Escherichia coli, K Fujiyama,S Sakuradani,DG Moran,T Yoshida,T Seki, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, SOC BIOSCIENCE BIOENGINEERING JAPAN, Vol. 91, No. 4, p. 419-421, 2001/04
  • Shigeo Shibatani, Kazuhito Fujiyama, Susumu Nishiguchi, Tatsuji Seki and Yoshihiko Maekawa : Production and characterization of an active and soluble human β1, 4-galactosyltransfease inEscherichia coli as a useful catalyst in the synthesis of Gal β1--&・・・, J. Biosci. Bioeng., Vol. , 91, 85-87/,, 2001
  • Ryo Misaki, Kazuhito Fujiyama, Yoshinobu Kimura and Tatsuji Seki : Structures of N-linked oligosaccharides of glycoproteins from spent medium of tobacco BY2 suspension cultured cells, Biosci. Biotechnol. Biochem., Vol. , 65, 2482-2488/,, 2001
  • Kazuhito Fujiyama, Nirianne Q Palacpac, Hiromi Sakai, Yoshinobu Kimura, Atsuhiko Shinmyo, Toshiomi Yoshida and Tatsuji Seki : In vivo Conversion of a Glycan to Human Compatible Type by transformed tobacco cells, Biochem. Biorhys. res. Commun, Vol. 289, 553-557/,, 2001
  • Production and characterization of active soluble human beta 1, 4-galactosyltransferase in Escherichia coli as a useful catalyst in synthesis of the Gal beta 1 -&gt; 4 GlcNAc linkage, S Shibatani,K Fujiyama,S Nishiguchi,T Seki,Y Maekawa, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, SOC BIOSCIENCE BIOENGINEERING JAPAN, Vol. 91, No. 1, p. 85-87, 2001/01
  • High expression of the penicillin G acylase gene (pac) from Bacillus megaterium UN1 in its own pac minus mutant, W Panbangred,K Weeradechapon,S Udomvaraphant,K Fujiyama,Meevootisom, V, JOURNAL OF APPLIED MICROBIOLOGY, BLACKWELL SCIENCE LTD, Vol. 89, No. 1, p. 152-157, 2000/07
  • High expression of the penicillin G acylase gene (pac) from Bacillus megaterium UN1 in its own pac minus mutant, W Panbangred,K Weeradechapon,S Udomvaraphant,K Fujiyama,Meevootisom, V, JOURNAL OF APPLIED MICROBIOLOGY, BLACKWELL SCIENCE LTD, Vol. 89, No. 1, p. 152-157, 2000/07
  • Purification and characterization of polyphenol oxidase from Muscat Bailey a grape juice, T Okuda,AG Pue,K Fujiyama,K Yokotsuka, AMERICAN JOURNAL OF ENOLOGY AND VITICULTURE, AMER SOC ENOLOGY VITICULTURE, Vol. 50, No. 2, p. 137-143, 1999
  • Stable expression of human β1,4-galactsosyltransferase gene in plant cells modifies N-glycosylation, FUJIYAMA Kazuhito, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Proc. Natl. Acad. Sci. U. S. A., Vol. 96, No. 8, p. 4692-4697, 1999
  • Structures of N-linked oligosaccharides of glycoproteins from tobacco BY2 suspension cultured cells, FUJIYAMA Kazuhito, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Biosci. Biotechnol. Biochem., Vol. 63, No. 1, p. 35-39, 1999
  • Characterization of recombinant human Man┣D29┫D2-mannosidase expressed in Escherichia coli, FUJIYAMA Kazuhito, JOURNAL OF FERMENTATION AND BIOENGINEERING, J. Ferment. Bioeng., Vol. 86, No. 3, p. 277-283, 1998
  • Untitled, K Fujiyama,T Seki, NUCLEIC ACIDS RESEARCH, OXFORD UNIV PRESS, Vol. 25, No. 9, p. U5-U5, 1997/05
  • Expression of glycoprotein processing enzymes in Escherichia coli as fusion proteins(共著), FUJIYAMA Kazuhito, WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, World Journal of Microbiology and Biotechnology, Vol. 13, No. 3, p. 265-267, 1997
  • Cloning of cDNA and charecten'zation of anti-RNase A monoclonal antibody(共著), FUJIYAMA Kazuhito, JOURNAL OF FERMENTATION AND BIOENGINEERING, Journal of Fermentation and Bioengineering, Vol. 82, No. 3, p. 312-314, 1996
  • On-line monitoring and control of Aceton-butanol fermentation by membrane-sensor mass spectrometry(共著), FUJIYAMA Kazuhito, JOURNAL OF FERMENTATION AND BIOENGINEERING, Journal of Fermentation and Bioengineering, Vol. 79, No. 3, p. 264-269, 1995
  • Purification and characterization of the recombinant alginate lyase from ┣DBPseudomonas(/)-┫DB sp. leaked by ┣DBEscherichia(/)-┫DB ┣DBcoli(/)-┫DB upon addition of glycine(共著), FUJIYAMA Kazuhito, FEMS MICROBIOLOGY LETTERS, FEMS Microbiology Letters, Vol. 126, No. 1, p. 19-23, 1995
  • High gene expression in ┣DBEscherichia col : (/)-┫DB of recombinant alginate lyase as a fused protein with β-galactosidase α-peptide(共著), FUJIYAMA Kazuhito, FEMS MICROBIOLOGY LETTERS, FEMS Microbiology Letters, Vol. 126, No. 1, p. 13-17, 1995
  • A QUANTITATIVE-ANALYSIS OF SHEAR EFFECTS ON CELL-SUSPENSION AND CELL-CULTURE OF PERILLA-FRUTESCENS IN BIOREACTORS, JJ ZHONG,K FUJIYAMA,T SEKI,T YOSHIDA, BIOTECHNOLOGY AND BIOENGINEERING, JOHN WILEY & SONS INC, Vol. 44, No. 5, p. 649-654, 1994/08
  • Expression and characterization of an engineered gene encoding horseradish peroxidase isozyme C1c(共著), FUJIYAMA Kazuhito, The Biochemical Society Meeting, The Biochemical Society Meeting, Vol. 653, 1994
  • Gene structure of peroxidase isozymes from horseradish and ┣DBArabidopsis thaliana(/)-┫DB(共著), FUJIYAMA Kazuhito, The Biochemical Society Meeting, The Biochemical Society Meeting, Vol. 653, 1994
  • A mass spectrometry membrane probe and practical problems associated with its application in Fermentation Processes(共著), FUJIYAMA Kazuhito, JOURNAL OF FERMENTATION AND BIOENGINEERING, Journal of Fermentation and Bioengineering, Vol. 79, No. 5, p. 465-472, 1994
  • The nylon oligomer biodegradation system of ┣DBFlavobacterium(/)-┫DB and ┣DBPseudomonas(/)-┫DB(共著), FUJIYAMA Kazuhito, Biodegradation, Biodegradation, Vol. 5, No. 3-4, p. 185-194, 1994
  • Characteristics of the Oligosaccharide structure of jack bean α-mannosidase with lectins(共著), FUJIYAMA Kazuhito, JOURNAL OF FERMENTATION AND BIOENGINEERING, Journal of Fermentation and Bioengineering, Vol. 78, No. 3, p. 255-258, 1994
  • On-line monitoring of cell concentration of ┣DBPerilla frutescens(/)-┫DB in a bioreactor, FUJIYAMA Kazuhito, BIOTECHNOLOGY AND BIOENGINEERING, Biotechnology & Bioengineering, Vol. 42, No. 4, p. 542-546, 1993
  • Enhancement of anthocyanin production by ┣DBPerilla frutescens(/)-┫DB cells in a stirred bioreactor with internal light irradiation, FUJIYAMA Kazuhito, JOURNAL OF FERMENTATION AND BIOENGINEERING, Journal of Fermentation and Bioengineering, Vol. 75, No. 4, p. 299-303, 1993
  • Cloning, sequence analysis and expression in ┣DBEscherichia(/)-┫DB ┣DBcoli(/)-┫DB of a gene encoding an alginote eyase from ┣DBPseudomonas(/)-┫DB sp. OS-ALG-9, FUJIYAMA Kazuhito, JOURNAL OF GENERAL MICROBIOLOGY, Journal of General Microbiology, Vol. 139, p. 987-993, 1993
  • CLONING OF CDNAS ENCODING 2 PEROXIDASES OF ARABIDOPSIS-THALIANA AND THEIR ORGAN-SPECIFIC EXPRESSION, C INTAPRUK,K YAMAMOTO,K FUJIYAMA,M TAKANO,A SHINMYO, JOURNAL OF FERMENTATION AND BIOENGINEERING, SOC FERMENTATION BIOENGINEERING, JAPAN, Vol. 75, No. 3, p. 166-172, 1993
  • Amino acid alterations essential for increasing the catalytic activity of the nylon-oligomer-degradation enzyme of ┣DBFlarobacterium(/)-┫DB sp., FUJIYAMA Kazuhito, EUROPEAN JOURNAL OF BIOCHEMISTRY, European Journal of Biochemistry, Vol. 200, No. 1, p. 165-169, 1991
  • Characterization of Hybrid Enzymes between 6-Aminohexanoate-Dimer Hydrolase and Its Analogous Protein, FUJIYAMA Kazuhito, JOURNAL OF FERMENTATION AND BIOENGINEERING, Journal of Fermentation and Bioengineering, Vol. 71, No. 5, p. 298-302, 1991
  • Alteration of Catalytic Function of 6-Aminohexanoate-Dimer Hydrolase by Site-directed Mutagenesis, FUJIYAMA Kazuhito, JOURNAL OF FERMENTATION AND BIOENGINEERING, Journal of Fermentation and Bioengineering, Vol. 71, No. 3, p. 191-193, 1991
  • Genomic DNA Structure of Two New Horseradish-Peroxidase-Encoding Genes, FUJIYAMA Kazuhito, GENE, Gene, Vol. 89, No. 2, p. 163-169, 1990
  • Structure of Horseradish Peroxidase Isozyme C Genes, FUJIYAMA Kazuhito, EUROPEAN JOURNAL OF BIOCHEMISTRY, European Journal of Biochemistry, Vol. 173, No. 3, p. 681-687, 1988
  • CONSTRUCTION OF HYBRID GENES OF 6-AMINOHEXANOIC ACID-OLIGOMER HYDROLASE AND ITS ANALOGOUS ENZYME - ESTIMATION OF THE INTRAMOLECULAR REGIONS IMPORTANT FOR THE ENZYME EVOLUTION, S NEGORO,S NAKAMURA,H KIMURA,K FUJIYAMA,YZ ZHANG,N KANZAKI,H OKADA, JOURNAL OF BIOLOGICAL CHEMISTRY, AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, Vol. 259, No. 22, p. 3648-3651, 1984
  • Tohru Okuda, Aisak Goliath Pue, Kazuhito Fujiyama, and Koki Yokotsuka: Purification and characterization of polyphenol oxidase from muscat bailey A grape juice.

Publications

  • 組換え医療用糖タンパク質の生産に向けた宿主細胞の育種, 藤山和仁, 酵素工学ニュース(酵素工学研究会), 2006/05
  • 植物生産医療タンパク質と糖鎖, 藤山和仁, 日本生物工学会, 2005/11
  • 植物バイオリアクターによる外来タンパク質生産 特集によせて, 藤山和仁, 日本生物工学会, 2005/11
  • 分子農業を目指した植物糖鎖工学, 藤山和仁, 財団法人 バイオインダストリー協会, 2004/12
  • Pharmaceuticals in Plants in Encyclopedia of Plants and Crop Science, Kazuhito Fujiyama, Dekker Inc, 2004/01
  • The analysis of antigen-antibody bindiug using anti-RNaseA single chain Fv-3A21(共著), Animal Cell Technology : Basic & Applied Aspects, 1994
  • Structure and expression of peroxidase isozyme genes in Horseradish and ┣DBArabidopsis(/)-┫DB, Plant Peroxidases : Biochemistry and Physiology University of Geneva, 1993
  • Structure of peroxidase isozyme genes in ┣DBBrassica(/)-┫DB and their expression, Biotechnology and Environmental Science : Molecular Approaches Plenum, Press, NY., 1992

Industrial Property Rights

  • 動物型糖鎖付加機能を持つ植物細胞, 谷口直之,関 達治,藤山和仁, 特願2001-44359, 出願日:2001/02
  • 動物型糖鎖付加機能をもつ植物細胞, 谷口直之,関 達治,藤山和仁, 特願2001-62704, 出願日:2001/03

Works

  • 亜熱帯微生物資源を活用する次世代物造りバイオ技術の構築, 2011 -
  • Next-genereation bioproduction platform leveraging subtropical microbial bioresources, 2011 -
  • 亜熱帯微生物資源を活用する次世代物造りバイオ技術の構築, 2009 -
  • Next-generation bioproduction platform leveraging subtropical microbial bioresources, 2009 -

Awards

  • 日本生物工学会論文賞, 菅沼 政俊・野村 雄・比嘉友紀子・片岡由起子・船隈 俊介・岡﨑 博之・鈴木 健夫・藤山 和仁・瀬筒 秀樹・立松謙一郎・田村 俊樹, 日本生物工学会, 2019/09
  • 日本生物工学会論文賞, 1999
  • 日本生物工学会論文賞, 藤山 和仁, 1994

Academic Activities

  • International Workshop on Plant-Made-Pharmaceuticals, 生物工学国際交流センター, 2014/09 -
  • 日本におけるイタリア2011・秋, イタリア政府, 2011/11 -
  • 新シルクロード: カイコゲノムから新しい農業へ, 2010/10 -
  • 「21 世紀最大の未利用資源活用のための昆虫・テクノロジー研究」プロジェクト シンポジウム, 独立行政法人農業生物資源研究所, 2006/09 -
  • 第24回 日本植物細胞分子生物学会(つくば)大会・シンポジウム, 日本植物細胞分子生物学会, 2006/07 -
  • 日本農芸化学会2006年度大会(北海道)「植物グリコミクスの鳥瞰と展望」, 日本農芸化学会, 2006/03 -
  • 糖鎖工学センターGGプロジェクトセミナー, 糖鎖工学センター, 2004/01 -
  • 国立感染症研究所学友会シンポジウム「QOL向上に貢献する植物科学への招待」, 国立感染症研究所学友会, 2001/11 -