High accumulation of the Man5GlcNAc2 structure by combining N-acetylglucosaminyltransferase I gene suppression and mannosidase I gene overexpression in Nicotiana tabacum SR1

Keigo Sato, Hitomi Yumioka, Junko Isoyama, Koji Dohi, Akihiro Yamanaka, Takao Ohashi, Ryo Misaki, Kazuhito Fujiyama

Journal of Bioscience and Bioengineering　Vol. 136　No. 2　p. 136-141　2023/08　Research paper (scientific journal)

High accumulation of the Man5GlcNAc2 structure by combining N-acetylglucosaminyltransferase I gene suppression and mannosidase I gene overexpression in Nicotiana tabacum SR1.

Keigo Sato, Hitomi Yumioka, Junko Isoyama, Koji Dohi, Akihiro Yamanaka, Takao Ohashi, Ryo Misaki, Kazuhito Fujiyama

Journal of bioscience and bioengineering　Vol. 136　No. 2　p. 136-141　2023/08　Research paper (scientific journal)

Production and N-glycan engineering of Varlilumab in Nicotiana benthamiana.

Kim Dua Nguyen, Hiroyuki Kajiura, Ryo Kamiya, Takahiro Yoshida, Ryo Misaki, Kazuhito Fujiyama

Frontiers in plant science　Vol. 14　p. 1215580-1215580　2023　Research paper (scientific journal)

Effects of various disaccharide adaptations on recombinant IgA1 production in CHO-K1 suspension cells

John Benson D. Choa, Tadahiro Sasaki, Hiroyuki Kajiura, Kazuyoshi Ikuta, Kazuhito Fujiyama, Ryo Misaki

Cytotechnology　2023　Research paper (scientific journal)

Insights into the quality of recombinant proteins produced by two different Bombyx mori expression systems.

Hiroyuki Kajiura, Ken-Ichiro Tatematsu, Tsuyoshi Nomura, Mitsuhiro Miyazawa, Akihiro Usami, Toshiki Tamura, Hideki Sezutsu, Kazuhito Fujiyama

Scientific reports　Vol. 12　No. 1　p. 18502-18502　2022/11/02　Research paper (scientific journal)

Impact of glycoengineering and antidrug antibodies on the anticancer activity of a plant-made lectin-Fc fusion protein.

Matthew Dent, Katarina L Mayer, Noel Verjan Garcia, Haixun Guo, Hiroyuki Kajiura, Kazuhito Fujiyama, Nobuyuki Matoba

Plant biotechnology journal　Vol. 20　No. 11　p. 2217-2230　2022/11　Research paper (scientific journal)

Mamestra brassicae NIAS-Mb-32 cell strain 2g2 enables high-yield recombinant protein production in baculovirus-free and baculovirus-based insect cell expression.

Yotaro Yamamoto, Hiroyuki Kajiura, Takahiro Nishibu, Kazuhito Fujiyama

Journal of bioscience and bioengineering　Vol. 134　No. 5　p. 432-440　2022/11　Research paper (scientific journal)

High-level transient production of a protease-resistant mutant form of human basic fibroblast growth factor in Nicotiana benthamiana leaves.

Edjohn Aaron Macauyag, Hiroyuki Kajiura, Takao Ohashi, Ryo Misaki, Kazuhito Fujiyama

Plant biotechnology (Tokyo, Japan)　Vol. 39　No. 3　p. 291-301　2022/09/25　Research paper (scientific journal)

Heterologous fusion gene expression and characterization of a novel carbohydrate binding module (Cbm36) to laccase (Lcc2)

Anita Kurniati, Ni Nyoman Tri Puspaningsih, Kartika Dwi Asni Putri, Mamik Damayanti, Ni Nyoman Purwani, Sylvia Aulia Rahmah, Purkan, Kazuhito Fujiyama, Makiko Sakka, Kazuo Sakka, Tetsuya Kimura, Ali Rohman, Afaf Baktir, Rahmat Eko Sanjaya

Biocatalysis and Agricultural Biotechnology　Vol. 42　p. 102377-102377　2022/07　Research paper (scientific journal)

Production of recombinant β-glucocerebrosidase in wild-type and glycoengineered transgenic Nicotiana benthamiana root cultures with different N-glycan profiles

Naphatsamon Uthailak, Hiroyuki Kajiura, Ryo Misaki, Kazuhito Fujiyama

Journal of Bioscience and Bioengineering　Vol. 133　No. 5　p. 481-488　2022/05　Research paper (scientific journal)

Effect of fruit maturation on N-glycosylation of plant-derived native and recombinant miraculin

Hiroyuki Kajiura, Kyoko Hiwasa-Tanase, Hiroshi Ezura, Kazuhito Fujiyama

Plant Physiology and Biochemistry　Vol. 178　p. 70-79　2022/05　Research paper (scientific journal)

Temporal analysis of N-acetylglucosamine extension of N-glycans in the middle silk gland of silkworm Bombyx mori.

Hiroyuki Kajiura, Takanori Eguchi, Keiro Uchino, Ken-Ichiro Tatematsu, Toshiki Tamura, Hideki Sezutsu, Kazuhito Fujiyama

Journal of bioscience and bioengineering　Vol. 133　No. 6　p. 533-540　2022/04/06　Research paper (scientific journal)

Establishment of serum-free adapted Chinese hamster ovary cells with double knockout of GDP-mannose-4,6-dehydratase and GDP-fucose transporter.

Ryo Misaki, Masashi Iwasaki, Hiroki Takechi, Noriko Yamano-Adachi, Takao Ohashi, Hiroyuki Kajiura, Kazuhito Fujiyama

Cytotechnology　Vol. 74　No. 1　p. 163-179　2022/02　Research paper (scientific journal)

Biochemical characterization of Arabidopsis clade F polygalacturonase shows a substrate preference toward oligogalacturonic acids

Takao Ohashi, Nabilah Sari, Ryo Misaki, Kazuhito Fujiyama

Journal of Bioscience and Bioengineering　Vol. 133　No. 1　p. 1-7　2022/01　Research paper (scientific journal)

Analysis of N-glycan profile of Arabidopsis alg3 cell culture.

Ratna Sariyatun, Hiroyuki Kajiura, Juthamard Limkul, Ryo Misaki, Kazuhito Fujiyama

Plant biotechnology (Tokyo, Japan)　Vol. 38　No. 4　p. 463-467　2021/12/25　Research paper (scientific journal)

Bombyx mori β1,4-N-acetylgalactosaminyltransferase possesses relaxed donor substrate specificity in N-glycan synthesis

Hiroyuki Kajiura, Ryousuke Miyauchi, Akemi Kakudo, Takao Ohashi, Ryo Misaki, Kazuhito Fujiyama

Scientific Reports　Vol. 11　No. 1　2021/12　Research paper (scientific journal)

Structure–function studies of ultrahigh molecular weight isoprenes provide key insights into their biosynthesis

Hiroyuki Kajiura, Takuya Yoshizawa, Yuji Tokumoto, Nobuaki Suzuki, Shinya Takeno, Kanokwan Jumtee Takeno, Takuya Yamashita, Shun-ichi Tanaka, Yoshinobu Kaneko, Kazuhito Fujiyama, Hiroyoshi Matsumura, Yoshihisa Nakazawa

Communications Biology　Vol. 4　No. 1　2021/12　Research paper (scientific journal)

Improved Assay System for Acidic Peptide: N-glycanase (aPNGase) Activity in Plant Extracts

Chiharu YAMAMOTO, Mikako OGURA, Ryota UEMURA, Megumi MAEDA, Hiroyuki KAJIURA, Ryo MISAKI, Kazuhito FUJIYAMA, Yoshinobu KIMURA

Anal Biochem.　p. 114367-114367　2021/09　Research paper (scientific journal)

Production of Human Acid-Alpha Glucosidase With a Paucimannose Structure by Glycoengineered Arabidopsis Cell Culture

Ratna Sariyatun, Florence, Hiroyuki Kajiura, Takao Ohashi, Ryo Misaki, Kazuhito Fujiyama

Frontiers in Plant Science　Vol. 12　2021/07/14　Research paper (scientific journal)

Transient Production of Human β-Glucocerebrosidase With Mannosidic-Type N-Glycan Structure in Glycoengineered Nicotiana benthamiana Plants

Naphatsamon Uthailak, Hiroyuki Kajiura, Ryo Misaki, Kazuhito Fujiyama

Frontiers in Plant Science　Vol. 12　2021/06/07　Research paper (scientific journal)

Direct evidence of cytosolic PNGase activity in Arabidopsis thaliana: in vitro assay system for plant cPNGase activity

Sahoko Shirai, Ryota Uemura, Megumi Maeda, Hiroyuki Kajiura, Ryo Misaki, Kazuhito Fujiyama, Yoshinobu Kimura

Bioscience, Biotechnology, and Biochemistry　Vol. 85　No. 6　p. 1460-1463　2021/05/25　Research paper (scientific journal)

Structure and Biological Functions of Plant Glycans and Polysaccharides

Ryo Misaki, Kazuhito Fujiyama, Hiroyuki Kajiura

Comprehensive Glycoscience　p. 93-109　2021　Part of collection (book)

Functional characterization and overexpression of Δ12-desaturase in the oleaginous yeast Rhodotorula toruloides for production of linoleic acid-rich lipids

Chih Chan Wu, Takao Ohashi, Hiroyuki Kajiura, Yu Sato, Ryo Misaki, Kohsuke Honda, Savitree Limtong, Kazuhito Fujiyama

Journal of Bioscience and Bioengineering　2021　Research paper (scientific journal)

Transglycosylation toward naringenin-7-O-glucoside using an N180H mutant of Coprinopsis cinerea endo-β-N-acetylglucosaminidase

Takao Ohashi, Yu Fujisawa, Marc R. Hayes, Ryo Misaki, Jörg Pietruszka, Kazuhito Fujiyama

Biochemical and Biophysical Research Communications　Vol. 530　No. 1　p. 155-159　2020/09/10　Research paper (scientific journal)

Characterization of Bombyx mori N-acetylglucosaminyltransferase II splicing variants

Hiroyuki Kajiura, Yugo Nakamura, Mari Nishimura, Takao Ohashi, Ryo Misaki, Kazuhito Fujiyama

Biochemical and Biophysical Research Communications　Vol. 529　No. 2　p. 404-410　2020/08　Research paper (scientific journal)

Enhancement of sialylation in rIgG in glyco-engineered Chinese hamster ovary cells

Nguyen TS, Misaki R, Ohashi T, Fujiyama K

Cytotechnology　Vol. 72　No. 3　p. 343-355　2020/07　Research paper (scientific journal)

Ethanol and H2O2 stresses enhance lipid production in an oleaginous Rhodotorula toruloides thermotolerant mutant L1-1.

Chih-Chan Wu, Takao Ohashi, Ryo Misaki, Savitree Limtong, Kazuhito Fujiyama

FEMS yeast research　Vol. 20　No. 4　2020/06/01　Research paper (scientific journal)

Fully Human Monoclonal Antibodies Effectively Neutralizing Botulinum Neurotoxin Serotype B

Matsumura T, Amatsu S, Misaki R, Yutani M, Anariwa D, Kohda T, Fujiyama K, Ikuta K, Fujinaga Y

Toxins　Vol. 12　No. 5　2020/05　Research paper (scientific journal)

Mating-type switching and mating-type gene array expression in the methylotrophic yeast Ogataea thermomethanolica TBRC656

Wongwisansri S, Promdonkoy P, Likhitrattanapisal S, Harnpichanchai P, Fujiyama K, Kaneko Y, Eurwilaichitr L, Ingsrisawang S, Tanapongpipat S

Microbiological Resarch　Vol. 232　2020/02　Research paper (scientific journal)

Biosynthetic Pathway of Indole-3-Acetic Acid in Basidiomycetous Yeast Rhodosporidiobolus fluvialis

Bunsangiam S, Sakpuntoon V, Srisuk N, Ohashi T, Fujiyama K, Limtong S

Mycobiology　Vol. 47　No. 3　p. 292-300　2019/07　Research paper (scientific journal)

Development of a Paper-Based Luminescence Bioassay for Therapeutic Monitoring of Aminoglycosides: a Proof-of-Concept Study.

Matsuura H, Ujiie K, Duyen TTM, Izutsu K, Fujiyama K, Maeda S, Harada K, Hirata K

Applied Biochemistry and Biotechnology　2019/05　Research paper (scientific journal)

The neutral N-linked glycans of the Basidiomycetous yeasts Pseudozyma antarctica and Malassezia furfur (Subphylum Ustilaginomycotina)

Flores RJD, Ohashi T, Sakai K, Gonoi T, Kawasaki H, Fujiyama K

J Gen Appl Microbiol　Vol. 65　No. 2　p. 53-63　2019/05　Research paper (scientific journal)

Delta-9 fatty acid desaturase overexpression enhanced lipid production and oleic acid content in Rhodosporidium toruloides for preferable yeast lipid production

Tsai YY, Ohashi T, Wu CC, Bataa D, Misaki R, Limtong S, Fujiyama K

J Biosci Bioeng　Vol. 127　No. 4　p. 430-440　2019/04　Research paper (scientific journal)

Technologies to elucidate functions of glycans

Koichi Furukawa, Yuhsuke Ohmi, Yuji Kondo, Yuki Ohkawa, Orie Tajima, Keiko Furukawa, Koichi Honke, Jin Ichi Inokuchi, Jianguo Gu, Kenji Kadomatsu, Satomi Nadanaka, Hiroshi Kitagawa, Shoko Nishihara, Kazuya Nomura, Shogo Oka, Makoto Ito, Ken Kitajima, Shunji Natsuka, Motoi Kanagawa, Takeshi Ishimizu, Kazuhito Fujiyama, Yasunori Chiba, Hiroyuki Osada

Glycoscience: Basic Science to Applications: Insights from the Japan Consortium for Glycobiology and Glycotechnology (JCGG)　p. 87-124　2019/01/01　Part of collection (book)

Arabidopsis thaliana α1,2-L-fucosyltransferase catalyzes the transfer of L-galactose to xyloglucan oligosaccharide

Ohashi H, Ohashi T, Misaki R, Fujiyama K

FEBS Lett　2019/01　Research paper (scientific journal)

Transcriptome sequencing and identification of cytochrome P450 monooxygenases involved in the biosynthesis of maslinic acid and corosolic acid in Avicennia marina.

Mitsuki Nakamura, Tran My Linh, Le Quynh Lien, Hayato Suzuki, Nguyen Chi Mai, Vu Huong Giang, Keita Tamura, Nguyen Van Thanh, Hideyuki Suzuki, Ryo Misaki, Toshiya Muranaka, Ninh Khac Ban, Kazuhito Fujiyama, Hikaru Seki

Plant biotechnology (Tokyo, Japan)　Vol. 35　No. 4　p. 341-348　2018/12/25　Research paper (scientific journal)

Comparison of the N-glycosylation on recombinant miraculin expressed in tomato plants with native miraculin

Tanase, Kyoko, Ezura, Hiroshi, Kajiura, Hiroyuki, Fujiyama, Kazuhito

Plant Biotechnology　2018/12　Research paper (scientific journal)

St6gal1 knockdown alters HBV life cycle in HepAD38 cells

Surya Agung Priyambada, Ryo Misaki, Toru Okamoto, Takao Ohashi, Keiji Ueda, Yoshiharu Matsuura, Kazuhito Fujiyama

Biochemical and Biophysical Research Communications　Vol. 503　No. 3　p. 1841-1847　2018/09　Research paper (scientific journal)

Isolation of a thermotolerant Rhodosporidium toruloides DMKU3-TK16 mutant and its fatty acid profile at high temperature.

Chih-Chan Wu, Yung-Yu Tsai, Takao Ohashi, Ryo Misaki, Savitree Limtong, Kazuhito Fujiyama

FEMS microbiology letters　Vol. 365　No. 21　2018/08　Research paper (scientific journal)

N-glycan sialylation in a silkworm-baculovirus expression system

Masatoshi Suganuma, Tsuyoshi Nomura, Yukiko Higa, Yukiko Kataoka, Shunsuke Funaguma, Hironobu Okazaki, Takeo Suzuki, Kazuhito Fujiyama, Hideki Sezutsu, Ken ichiro Tatematsu, Toshiki Tamura

Journal of Bioscience and Bioengineering　Vol. 126　No. 1　p. 9-14　2018/07　Research paper (scientific journal)

The production of human β-glucocerebrosidase in Nicotiana benthamiana root culture

Naphatsamon U, Ohashi T, Misaki R, Fujiyama K

Int J Mol Sci　2018/07　Research paper (scientific journal)

Enhancement of glycosylation by stable co-expression of two sialylation-related enzymes on Chinese hamster ovary cells

Nguyen TS, Misaki R, Ohashi T, Fujiyama K

J Biosci Bioeng　2018/07　Research paper (scientific journal)

Hydroponic treatment of Nicotiana benthamiana with kifunensine modifies the N-glycans of recombinant glycoprotein antigens to predominantly Man9 high-mannose type upon transient overexpression.

Roychowdhury S, Oh YJ, Kajiura H, Hamorsky KT, Fujiyama K, Matoba N

Front Plant Sci.　Vol. 9　p. 62-62　2018/04　Research paper (scientific journal)

Molecular cloning and overexpression of DGA1, an acyl-CoA dependent diacylglycerol acyltransferase, in the oleaginous yeast Rhodosporidiobolus fluvialis DMKU-RK253

Polburee P, Ohashi T, Tsai YY, Sumyai T, Lertwattanasakul N, Limtong S, Fujiyama K

Microbiology　2018/01　Research paper (scientific journal)

Isolation and characterization of a stress-responsive gene encoding a CHRD domain-containing protein from a halotolerant green alga

Ryo Ishinishi, Hideyuki Matsuura, Satoshi Tanaka, Saaya Nozawa, Keisuke Tanada, Norihito Kawashita, Kazuhito Fujiyama, Hitoshi Miyasaka, Kazumasa Hirata

GENE　Vol. 640　p. 14-20　2018/01　Research paper (scientific journal)

Effective Synthesis of Guanosine 5’-Diphospho-β-L-galactose Using Bacterial L-Fucokinase/Guanosine 5’-diphosphate-L-fucose Pyrophosphorylase

Ohashi H, Wahl C, Ohashi T, Elling L, Fujiyama K

Adv Synth Catal　2017/10　Research paper (scientific journal)

A polygalacturonase localized in the Golgi apparatus in Pisum sativum

Ohashi, T, Jinno, J, Inoue, Y, Ito, S, Fujiyama, K, Ishimizu, T

J. Biochem.　Vol. 161　No. 6　2017/07

Cell surface N-glycan alteration in HepAD38 cell lines expressing Hepatitis B virus

Surya Agung Priyambada, Ryo Misaki, Toru Okamoto, Yuta Okamoto, Takao Ohashi, Keiji Ueda, Yoshiharu Matsuura, Kazuhito Fujiyama

Virus Research　Vol. 238　p. 101-109　2017/06/15　Research paper (scientific journal)

Erratum to: Development of a sufficient and effective procedure for transformation of an oleaginous yeast, Rhodosporidium toruloides DMKU3-TK16 (Current Genetics, (2017), 63, 2, (359-371), 10.1007/s00294-016-0629-8)

Yung-Yu Tsai, Takao Ohashi, Takenori Kanazawa, Pirapan Polburee, Ryo Misaki, Savitree Limtong, Kazuhito Fujiyama

Current Genetics　Vol. 63　No. 2　2017/05/01　Research paper (scientific journal)

Biochemical characterization of rhamnosyltransferase involved in biosynthesis of pectic rhamnogalacturonan I in plant cell wall

Uehara Y, Tamura S, Maki Y, Yagyu K, Mizoguchi T, Tamiaki H, Imai T, Ishii T, Ohashi T, Fujiyama K, Ishimizu T

Biochem Biophys Res Commun　2017/04　Research paper (scientific journal)

The neutral N-linked glycans of the Ustilaginomycetes yeast Sympodiomycopsis pahiopedili

Flores RJD, Ohashi T, Kawasaki H, Fujiyama K

Yeast　2017/04　Research paper (scientific journal)

Two Eucommia farnesyl diphosphate synthases exhibit distinct enzymatic properties leading to end product preferences.

Kajiura H, Suzuki N, Tokumoto Y, Yoshizawa T, Takeno S, Fujiyama K, Kaneko Y, Matsumura H, Nakazawa Y

Biochimie　Vol. 139　p. 95-106-106　2017/04　Research paper (scientific journal)

N-glycan structures and downstream mannose-phosphorylation of plant recombinant human alpha-L-iduronidase: toward development of enzyme replacement therapy for mucopolysaccharidosis I.

Pierce OM, McNair GR, He X, Kajiura H, Fujiyama K, Kermode AR

Plant Mol Biol.　Vol. 95　No. 6　p. 593-606-606　2017/04　Research paper (scientific journal)

A polygalacturonase localized in the Golgi apparatus in Pisum sativum

Ohashi T, Jinno J, Inoue Y, Ito S, Fujiyama K, Ishimizu T

J Biochem　2017/02　Research paper (scientific journal)

Fucosyltransferases produce N-glycans containing core l-galactose.

Hiroyuki Ohashi, Takao Ohashi, Hiroyuki Kajiura, Ryo Misaki, Shinichi Kitamura, Kazuhito Fujiyama

Biochemical and biophysical research communications　Vol. 483　No. 1　p. 658-663　2017/01　Research paper (scientific journal)

The production of human glucocerebrosidase in glyco-engineered Nicotiana benthamiana plants.

Juthamard Limkul, Sayoko Iizuka, Yohei Sato, Ryo Misaki, Takao Ohashi, Toya Ohashi, Kazuhito Fujiyama

Plant biotechnology journal　Vol. 14　No. 8　p. 1682-94　2016/08　Research paper (scientific journal)

Lipid production from biodiesel-derived crude glycerol by Rhodosporidium fluviale DMKU-RK253 using temperature shift with high cell density

Pirapan Polburee, Wichien Yongmanitchai, Kohsuke Honda, Takao Ohashi, Toshiomi Yoshida, Kazuhito Fujiyama, Savitree Limtong

BIOCHEMICAL ENGINEERING JOURNAL　Vol. 112　p. 208-218　2016/08　Research paper (scientific journal)

Development of a sufficient and effective procedure for transformation of an oleaginous yeast, Rhodosporidium toruloides DMKU3‑TK16

Tsai Y-Y, Ohashi T, Kanazawa T, Polburee P, Misaki R, Limtong S, Fujiyama K

Curr Genet　2016/07　Research paper (scientific journal)

Core-fucosylation plays a pivotal role in hepatitis B pseudo virus infection: a possible implication for HBV glycotherapy

Shinji Takamatsu, Mayuka Shimomura, Yoshihiro Kamada, Haruka Maeda, Tomoaki Sobajima, Hayato Hikita, Masumi Iijima, Yuta Okamoto, Ryo Misaki, Kazuhito Fujiyama, Shushi Nagamori, Yoshikatsu Kanai, Tetsuo Takehara, Keiji Ueda, Shun'ichi Kuroda, Eiji Miyoshi

Glycobiology　2016/06/21　Research paper (scientific journal)

Altered glycosylation of exported proteins,including surface immune receptors, compromises calcium and downstream signaling responses to microbe-associated molecular patterns in Arabidopsis thaliana.

Trempel F, Kajiura H, Ranf S, Grimmer J, Westphal L, Zipfel C, Scheel D, Fujiyama K, Lee J

BMC Plant Biol.　Vol. 16　2016/04　Research paper (scientific journal)

Recombinant production and characterization of human anti-influenza virus monoclonal antibodies identified from hybridomas fused with human lymphocytes.

Misaki R, Fukura N, Kajiura H, Yasugi M, Kubota-Koketsu R, Sasaki T, Momota M, Ono K, Ohashi T, Ikuta K, Fujiyama K

Biologicals　Vol. 44　2016/04

The combination of plant translational enhancers and terminator increase the expression of human glucocerebrosidase in Nicotiana benthamiana plants

Juthamard Limkul, Ryo Misaki, Ko Kato, Kazuhito Fujiyama

Plant Science　Vol. 240　p. 41-49　2015/11/01　Research paper (scientific journal)

A novel potential signal peptide sequence and overexpression of ER-resident chaperones enhance heterologous protein secretion in thermotolerant methylotrophic yeast Ogataea thermomethanolica

Roongsawang N, Puseenam A, Kitikhun S, Sae-Tang K, Harnpicharnchai P, Ohashi T, Fujiyama K, Tirasophon W, Tanapongpipat S

Appl Biochem Biotechnol　2015/10　Research paper (scientific journal)

Substrate preference of citrus naringenin rhamnosyltransferases and their application to flavonoid glycoside production in fission yeast

Takao Ohashi, Yuka Hasegawa, Ryo Misaki, Kazuhito Fujiyama

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY　Vol. 100　No. 2　p. 687-696　2015/10　Research paper (scientific journal)

Characterization of oleaginous yeasts accumulating high levels of lipid when cultivated in glycerol and their potential for lipid production from biodiesel-derived crude glycerol

Polburee P, Yongmanitchai W, Lertwattanasakul N, Ohashi T, Fujiyama K, Limtong S

Fungal Biol　2015/09　Research paper (scientific journal)

N-glycosylation of cholera toxin B subunit in Nicotiana benthamiana: impacts on host stress response, production yield and vaccine potential.

Hamorsky KT, Kouokam JC, Jurkiewicz JM, Nelson B, Moore LJ, Husk AS, Kajiura H, Fujiyama K, Matoba N

Sci Rep.　Vol. 23　2015/04　Research paper (scientific journal)

Im"plant"ing of mammalian glycosyltransferase gene into plant suspension-cultured cells using Agrobacterium-mediated transformation.

Kajiura H, Fujiyama K

Methods Mol Biol.　Vol. 1321　p. 225-232-232　2015/04　Research paper (scientific journal)

Production of initial-stage eukaryotic N-glycan and its protein glycosylation in Escherichia coli

Akkaraphol Srichaisupakit, Takao Ohashi, Ryo Misaki, Kazuhito Fujiyama

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　Vol. 119　No. 4　p. 399-405　2015/04　Research paper (scientific journal)

Sialylation potentials of the silkworm, Bombyx mori; B.mori possesses an active α2,6-sialyltransferase.

Kajiura H, Hamaguchi Y, Mizushima H, Misaki R, Fujiyama K

Glycobiology　Vol. 25　No. 12　p. 1441-1453-1453　2015/04　Research paper (scientific journal)

Cloning and expression analysis of acidic peptide:N-glycanase genes in Solanum lycoperisicum.

Fujikawa, M, Maeda, M, Fujiyama , K, Kimura, Y

Glycoconjugate Journal　Vol. 32　No. 5　2015

Barnettozyma siamensis f.a., sp. nov., a lipid-accumulating ascomycete yeast species

Polburee P, Yongmanitchai W, Ohashi T, Fujiyama K, Limtong S

Int J Syst Evol Microbiol　Vol. 64　No. Pt 9　p. 3053-3057　2014/09　Research paper (scientific journal)

Identification of a protein glycosylation operon from Campylobacter jejuni JCM 2013 and its heterologous expression in Escherichia coli

Srichaisupakit Akkaraphol, Ohashi Takao, Fujiyama Kazuhito

Journal of bioscience and bioengineering　Vol. 118　No. 3　p. 256-262　2014/09　Research paper (scientific journal)

Antibody germline characterization of cross-neutralizing human IgGs against 4 serotypes of dengue virus.

Pannamthip Pitaksajjakul, Surachet Benjathummarak, Cholnatip Pipattanaboon, Waranya Wongwit, Tamaki Okabayashi, Motoki Kuhara, Ryo Misaki, Kazuhito Fujiyama, Pongrama Ramasoota

Biochemical and Biophysical Research Communicatioins　Vol. 446　No. 2　p. 475-480　2014/04　Research paper (scientific journal)

Insight of Plant N-glycosylation Pathway through Heterologously—Expressed Enzymes

KAJIURA Hiroyuki, FUJIYAMA Kazuhito

KAGAKU TO SEIBUTSU　Vol. 52　No. 11　p. 719-724　2014

Cloning of a cDNA encoding the Gly m Bd 28K precursor and its vacuole transport in tobacco BY2 suspension-cultured cells.

Hitomi Yumioka-Ito, Ryo Misaki, Miyuki Yokoro, Makiko Suzuki, Hiromi Yamashita, Miki Hiemori-Kondo, Masumi Kimoto, Ko Kato, Kazuhito Fujiyama, Hideaki Tsuji

Journal of nutritional science and vitaminology　Vol. 60　No. 2　p. 129-39　2014　Research paper (scientific journal)

Emerging Antigenic Variants at the Antigenic Site Sb in Pandemic A(H1N1)2009 Influenza Virus in Japan Detected by a Human Monoclonal Antibody

M. Yasugi, R. Kubota-Koketsu, A. Yamashita, N. Kawashita, A. Du, R. Misaki, M. Kuhara, N. Boonsathorn, K. Fujiyama, Y. Okuno, T. Nakaya, K. Ikuta

PLoS One　Vol. 8　No. 10　2013/10　Research paper (scientific journal)

Dengue virus neutralization and antibody-ependetn enhancement activities of human monoclonal antibodies derived from dengue patiensts as acute phase of secondary infections.

Tadahiro Sasaki, Chayanee Setthapramote, Takeshi Kurosu, Mituhiro Nishimura, Azusa Asai, Tamaki Okabayashi, Magot Omokoko, Chonlatip Pipattanaboon, Pannnamthip Pitaksajjakul, Kriengsak, Limittikul, Arunee Subchareon, Panjaporn Chaichana, Tamaki Okabayashi, Itaru Hirai, Pornsawan Leaungwutiwong, Ryo Misaki, Kazuhiro Fujiyama, Ken-ichiro Ono, Yoshinobu Okuno, Pongrama Ramasoota, Kazuyoshi Ikuta

Antiviral Research　Vol. 10　p. 1016-1016　2013/06　Research paper (scientific journal)

Rapid and scalable plant-based production of a cholera toxin B subunit variant to aid in mass vaccination against cholera outbreaks.

Hamorsky KT, Kouokam JC, Bennett LJ, Baldauf KJ, Kajiura H, Fujiyama K, Matoba N

PLoS Negl Trop Dis.　Vol. 7　No. 3　2013/04　Research paper (scientific journal)

Transgenic Rice Seeds Accumulating Recombinant Hypoallergenic Birch Pollen Allergen Bet v 1 Generate Giant Protein Bodies.

Wang S, Takahashi H, Kajiura H, Kawakatsu T, Fujiyama K, Takaiwa F

Plant Cell Physiol.　Vol. 54　2013/04　Research paper (scientific journal)

N-Glycosylation and N-Glycan moieties of CTB expressed in rice seeds.

Kajiura H, Wasai M, Kasahara S, Takaiwa F, Fujiyama K

Mol Biotechnol.　Vol. 54　No. 3　p. 784-794-794　2013/04　Research paper (scientific journal)

Human monoclonal antibodies broadly neutralizing against influenza B virus.

Yasugi M, Kubota-Koketsu R, Yamashita A, Kawashita N, Du A, Sasaki T, Nishimura M, Misaki R, Kuhara M, Boonsathorn N, Fujiyama K, Okuno Y, Nakaya T, Ikuta K

PLoS Pathog　Vol. 9　No. 2　2013/02　Research paper (scientific journal)

Identification of Novel alpha 1,3-Galactosyltransferase and Elimination of alpha-Galactose-containing Glycans by Disruption of Multiple alpha-Galactosyltransferase Genes in Schizosaccharomyces pombe

Takao Ohashi, Kazuhito Fujiyama, Kaoru Takegawa

JOURNAL OF BIOLOGICAL CHEMISTRY　Vol. 287　No. 46　p. 38866-38875　2012/11　Research paper (scientific journal)

Characterization of URA3 gene in oleaginous yeast Rhodosporidium toruloides DMKU3-TK16 for lipid engineering

Takenori Kanazawa, Takao Ohashi, Ayako Tomio, Ryo Misaki, Takeshi Bamba, Eiichiro Fukusaki, Limtong Savitree, Kazuhito Fujiyama

2012/10

Production of different glycosylation variants of the tumour-targeting mAb H10 in Nicotiana benthamiana: influence on expression yield and antibody degradation.

Lombardi R, Donini M, Villani ME, Brunetti P, Fujiyama K, Kajiura H, Paul M, Ma JK, Benvenuto E

Transgenic Res.　Vol. 21　2012/04　Research paper (scientific journal)

Evolutionarily conserved glycan signal to degrade aberrant brassinosteroid receptors in Arabidopsis.

Hong Z, Kajiura H, Su W, Jin H, Kimura A, Fujiyama K,Li J

Proc Natl Acad Sci U S A　Vol. 109　2012/04　Research paper (scientific journal)

N-Glycan structures of glycoproteins in suspension-cultured Arabidopsis thaliana T87 cells.

Kajiura H, Tanaka R, Kato K, Hamaguchi Y, Seki T, Fujiyama K

Plant Biotechnol.　Vol. 29　No. 5　p. 489-493-493　2012/04　Research paper (scientific journal)

Enhancement of sialylation on humanized IgG-like bispecific antibody by overexpression of alpha2,6-sialyltransferase derived from Chinese hamster ovary cells.

Onitsuka M, Kim WD, Ozaki H, Kawaguchi A, Honda K, Kajiura H, Fujiyama K, Asano R, Kumagai I, Ohtake H, Omasa T

Appl Microbiol Biotechnol.　Vol. 94　No. 1　p. 69-80-80　2012/04　Research paper (scientific journal)

Arabidopsis beta 1,2-xylosyltransferase: Substrate specificity and participation in the plant-specific N-glycosylation pathway

Hiroyuki Kajiura, Toru Okamoto, Ryo Misaki, Yoshiharu Matsuura, Kazuhito Fujiyama

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　Vol. 113　No. 1　p. 48-54　2012/01　Research paper (scientific journal)

N-terminal vacuolar sorting signal at the mouse antibody alters the N-linked glycosylation pattern in suspension-cultured tobacco BY2 cells

Ryo Misaki, Yohei Sakai, Takeshi Omasa, Kazuhito Fujiyama, Tatsuji Seki

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　Vol. 112　No. 5　p. 476-484　2011/11　Research paper (scientific journal)

Improved expression and characterization of recombinant human Golgi alpha 1, 2-mannosidase I isoforms (IA2 and IC) by Escherichia coli

Junko Isoyama-Tanaka, Koji Dohi, Ryo Misaki, Kazuhito Fujiyama

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　Vol. 112　No. 1　p. 14-19　2011/07　Research paper (scientific journal)

Reduced Immunogenicity of Arabidopsis hgl1 Mutant N-Glycans Caused by Altered Accessibility of Xylose and core Fucose Epitopes

Heidi Kaulfuerst-Soboll, Stephan Rips, Hisashi Koiwa, Hiroyuki Kajiura, Kazuhito Fujiyama, Antje von Schaewen

JOURNAL OF BIOLOGICAL CHEMISTRY　Vol. 286　No. 26　p. 22955-22964　2011/07　Research paper (scientific journal)

Double-Knockout of Putative Endo-beta-N-acetylglucosaminidase (ENGase) Genes in Arabidopsis thaliana: Loss of ENGase Activity Induced Accumulation of High-Mannose Type Free N-Glycans Bearing N,N '-Acetylchitobiosyl Unit

Yoshinobu Kimura, Yuki Takeoka, Masami Inoue, Megumi Maeda, Kazuhito Fujiyama

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY　Vol. 75　No. 5　p. 1019-1021　2011/05　Research paper (scientific journal)

Reduced immunogenicity of Arabidopsis hybrid glycosylation1 (hgl1) N-glycans due to altered accessibility of xylose and core fucose epitopes.

Kaulfuerst-Soboll H, Rips S, Koiwa H, Kajiura H, Fujiyama K, von Schaewen A

J Biol Chem.　Vol. 286　2011/04　Research paper (scientific journal)

Stable coexpression of two human sialylation enzymes in plant suspension-cultured tobacco cells.

Kajiura H, Misaki R, Fujiyama K, Seki T

J Biosci Bioeng.　Vol. 111　No. 4　p. 471-477-477　2011/04　Research paper (scientific journal)

Jack bean alpha-mannosidase digestion profile of hybrid-type N-glycans: Effect of reaction pH on substrate preference

Koji Dohi, Junko Isoyama-Tanaka, Ryo Misaki, Kazuhito Fujiyama

BIOCHIMIE　Vol. 93　No. 4　p. 766-771　2011/04　Research paper (scientific journal)

P-62 Development of intelligent cultural system for extreme environmental conditions

HAYASHI Jun, TAKEISHI Hiroyuki, NAKATSUKA Noriaki, SUDA Yoshito, MACHIMURA Tadashi, OKAZAWA Atsushi, HARADA Kazuo, HIRATA Kazumasa, Fujiyama kazuhito, KOBAYASHI Akio, AKAMATSU Fumiteru

Proceedings of the Conference on Biomass Science　Vol. 6　p. 176-177　2011

Rice Golgi proteome : identification and characterization of N-acetylglucosaminyltransferase-I-like protein.

Mitsui T, Asakura T, Nakayama Y, Takayama K, Hirose S, Katamine H, Aoyama M, Karahashi A, Kaneko K, Ishiyama R, Itoh K, Hori H, Kajiura H, Fujiyama K

Bull. Facul. Agric. Niigata Univ.　Vol. 63　2010/04　Research paper (scientific journal)

The impact of the overexpression of human UDP-galactose transporter gene hUGT1 in tobacco plants.

Khalil MF, Kajiura H, Fujiyama K, Koike K, Ishida N, Tanaka N

J Biosci Bioeng.　Vol. 109　2010/04　Research paper (scientific journal)

Arabidopsis thaliana ALG3 mutant synthesizes immature oligosaccharides in the ER and accumulates unique N-glycans.

Kajiura H, Seki T, Fujiyama K

Glycobiology　Vol. 20　No. 6　p. 736-751-751　2010/04　Research paper (scientific journal)

Two Arabidopsis thaliana Golgi alpha-mannosidase I enzymes are responsible for plant N-glycan maturation

Hiroyuki Kajiura, Hisashi Koiwa, Yoshihisa Nakazawa, Atsushi Okazawa, Akio Kobayashi, Tatsuji Seki, Kazuhito Fujiyama

GLYCOBIOLOGY　Vol. 20　No. 2　p. 235-247　2010/02　Research paper (scientific journal)

Wickerhamomyces edaphicus sp. nov. and Pichia jaroonii sp. nov., two ascomycetous yeast species isolated from forest soil in Thailand

Savitree Limtong, Wichien Yongmanitchai, Hiroko Kawasaki, Kazuhito Fujiyama

FEMS Yeast Research　Vol. 9　No. 3　p. 504-510　2009/05　Research paper (scientific journal)

Biochemical and immunological characterization of the plant-derived candidate human immunodeficiency virus type 1 mucosal vaccine CTB-MPR.

Matoba N, Kajiura H, Cherni I, Doran JD, Bomsel M, Fujiyama K, Mor TS

Plant Biotechnol J.　Vol. 7　2009/04　Research paper (scientific journal)

Cloning and characterization of cytidine monophosphate-3-deoxy-D-manno-octulosonate synthetase from Arabidopsis thaliana.

Misaki R, Kajiura H, Fujii K, Fujiyama K, Seki T

J Biosci Bioeng.　Vol. 108　No. 6　p. 527-529-529　2009/04　Research paper (scientific journal)

Molecular Cloning and Gene Expression Analysis of Tomato Endo-beta-N-acetylglucosaminidase, an Endoglycosidase Involved in the Production of High-Mannose Type Free N-Glycans during Tomato Fruit Ripening

Kosuke Nakamura, Masami Inoue, Megumi Maeda, Ryohei Nakano, Katsutoshi Hosoi, Kazuhito Fujiyama, Yoshinobu Kimura

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY　Vol. 73　No. 2　p. 461-464　2009/02　Research paper (scientific journal)

Change in glycosylation pattern with extension of endoplasmic reticulum retention signal sequence of mouse antibody produced by suspension-cultured tobacco BY2 cells

Kazuhito Fujiyama, Ryo Misaki, Yohei Sakai, Takeshi Omasa, Tatsuji Seki

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　Vol. 107　No. 2　p. 165-172　2009/02　Research paper (scientific journal)

Analysis of CMP-sialic acid transporter-like proteins in plants

Shou Takashima, Junichi Seino, Takeshi Nakano, Kazuhito Fujiyama, MasafumiTsujimoto, Nobuhiro Ishida, Yasuhiro Hashimoto

Phytochemistry　Vol. 70　p. 1973-1981　2009/01　Research paper (scientific journal)

Generation of a transgenic rice seed-based edible vaccine against house dust mite allergy.

Yang L, Kajiura H, Suzuki K, Hirose S, Fujiyama K, Takaiwa F

Biochem Biophys Res Commun.　Vol. 365　2008/04　Research paper (scientific journal)

Salt tolerance of Arabidopsis thaliana requires maturation of N-glycosylated proteins in the Golgi apparatus.

Kang JS, Frank J, Kang CH, Kajiura H, Vikram M, Ueda A, Kim S, Bahk JD, Triplett B, Fujiyama K, Lee SY, von, Schaewen A, Koiwa H

Proc Natl Acad Sci U S A　Vol. 105　p. 5933-5938-5938　2008/04　Research paper (scientific journal)

Salt tolerance of Arabidopsis thaliana requires maturation of N-glycosylated proteins in the Golgi

J. S. Kang, J. Frank, C. H. Kang, H. Kajiura, M. Vikram, A. Ueda, S. Kim, J. D. Bahk, B. Triplett, K. Fujiyama, S. Y. Lee, A. von Schaewen, H.i Koiwa

Proc Natl Acad Sci USA.　Vol. 105　No. 15　p. 5933-8　2008/04　Research paper (scientific journal)

Biochemical and immunological characterization of the plant-derived candidate human immunodeficiency virus type 1 mucosal vaccine CTB-MPR649-684

Nobuyuki Matoba, Hiroyuki Kajiura, Irene Cherni, Jeffrey D Doran, Morgane Bomsel, Kazuhito Fujiyama, Tsafrir S Mor

Plant Biotechnol J　2008/02　Research paper (scientific journal)

Salt-adaptation of Tobacco BY2 Cells Induces Change in Glycoform of N-Glycans: Enhancement of Exo- and Endo-glycosidase Activities by Salt-adaptation.

Y. Kimura, T. Watanabe, M. Maeda, Y. Murata, K. Fujiyama

Biosci. Biotechnol. Biochem.　Vol. 72　No. 2　p. 514-522　2008/02　Research paper (scientific journal)

Generation of a transgenic rice seed-based edible vaccine against house dust mite allergy

L. Yang, H. Kajiura, K. Suzuki, S. Hirose, K. Fujiyama, F. Takaiwa

Biochem. Biophys. Res. Commun.　2007/10　Research paper (scientific journal)

Molecular Cloning and Expression of Two Novel beta-N-acetylglucosaminidases from Silkworm Bombyx mori

T. Okada, S. Ishiyama, H. Sezutsu, A. Usami, T. Tamura, K. Mita, K. Fujiyama, T. Seki

Biosci. Biotechnol. Biochem.　Vol. 71　No. 7　p. 1626-1635　2007/07　Research paper (scientific journal)

First Evidence for 1-3 Galactosyltransferase Activity Involved in Biosynthesis of Insect Complex Type N-Glycan Harboring T-Antigen in Apis mellifera

Y. Kimura, S. Sakamura, T. Ushijima, Y. Hama, H. Kajiura, K. Fujiyama, K. Okihara, K. Hashimoto, H. Sugimoto, H. Yamada

Biosci. Biotechnol. Biochem.　2007/04　Research paper (scientific journal)

Evidence for new beta1-3 galactosyltransferase activity involved in biosynthesis of unusual N-glycan harboring T-antigen in Apis mellifera.

Kimura Y, Sakamura S, Ushijima T, Hama Y, Kajiura H, Fujiyama K, Okihara K, Hashimoto K, Sugimoto H, Yamada H

Biosci Biotechnol Biochem.　Vol. 71　No. 4　p. 1111-1114-1114　2007/04　Research paper (scientific journal)

Construction of Lactobacillus plantarum strain with enhanced L-lysine yield

M. N. Cahyanto, H. Kawasaki, M. Nagashio, K. Fujiyama, T. Seki

JOURNAL OF APPLIED MICROBIOLOGY　Vol. 102　No. 3　p. 674-679　2007/02　Research paper (scientific journal)

Production in plant of dengue 2 envelope domain III using TMV-based vector system

W. Saejung, K. Fujiyama, K. Hori, Y. Watanabe, T. Takasaki, I. Kurane, P. Malasit, T. Seki

Vaccine　Vol. 25　p. 6646-6654　2007/01　Research paper (scientific journal)

Replication-Competent Recombinant Vesicular Stomatitis Virus Encoding Hepatitis C Virus Envelope Proteins

H. Tani, Y. Komoda, E. Matsuo, K. Suzuki, I. Hamamoto, T. Yamashita, K. Moriishi, K. Fujiyama, T. Kanto, N. Hayashi, A. Owsianka, A. H. Patel, M. A. Whitt, Y.u Matsuura

J. Virology　Vol. 81　p. 8601-8612　2007/01　Research paper (scientific journal)

Production of mouse monoclonal antibody with galactose-extended sugar chain by suspension cultured tobacco BY2 cells expressing human b(1,4)-galactosyltransferase

K. Fujiyama, A. Furukawa, A. Katsura, R. Misaki, T. Omasa, T. Seki

Biochem. Biophys. Res. Commun.　Vol. 358　No. 1　p. 85-91　2007/01　Research paper (scientific journal)

In planta production of immunogenic poliovirus peptide using tobacco mosaic virus-based vector system

Kazuhito Fujiyama, Wanida Saejung, Itaru Yanagihara, Junko Nakado, Ryo Misaki, Takeshi Honda, Yuichiro Watanabe, Tatsuji Seki

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　Vol. 101　No. 5　p. 398-402　2006/11　Research paper (scientific journal)

Production of Soluble Recombinant Dengue Virus 2 Envelope Domain III Protein in Escherichia coli

W. Saejung, C. Puttikhunt, T. Prommool, P. Sojikul, R. Tanaka, K. Fujiyama, P. Malasit, T. Seki

2006/10

Enhancement of recombinant soluble dengue virus 2 envelope domain III protein production Escherichia coli trxB and gor double mutant

Wanida Saejung, Chunya Puttikhunt, Tanapan Prommool, Punchapat Sojikul, Ryoji Tanaka, Kazuhito Fujiyama, Prida Malasit, Tatsuji Seki

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　Vol. 102　No. 4　p. 333-339　2006/10　Research paper (scientific journal)

N-Linked Glycan Structures of a Mouse Monoclonal Antibody Produced from Tobacco BY2 Suspension-Cultured Cells

Fujiyama Kazuhito, Misaki Ryo, Katsura Atsuya, Tanaka Tomoko, Furukawa Aiko, Omasa Takeshi, Seki Tatsuji

Journal of Fermentation Technology　Vol. 101　No. 3　p. 212-218　2006/03　Research paper (scientific journal)

Isolation and characterization of nitrogen-fixing bacteria from soil and rhizosphere

Khiev P, 木谷茂, 木下浩, 川崎浩子, 藤山和仁, 仁平卓也, 関達治, Panbangred W

ユネスコバイオテクノロジー国際大学院研修講座第一期実施報告書　2006/03　Research paper (bulletin of university, research institution)

Expression of human CMP-N-acetylneuraminic acid synthetase and CMP-sialic acid transporter in tobacco suspension-cultured cell

R Misaki, K Fujiyama, T Seki

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　Vol. 339　No. 4　p. 1184-1189　2006/01　Research paper (scientific journal)

Process development and economic evaluation of recombinant human lactoferrin expressed in rice grain

S Nandi, D Yalda, S Lu, Z Nikolov, R Misaki, K Fujiyama, N Huang

TRANSGENIC RESEARCH　Vol. 14　No. 3　p. 237-249　2005/06　Research paper (scientific journal)

N-linked glycan structures of human lactoferrin produced by transgenic rice

K Fujiyama, Y Sakai, R Misaki, Yanagihara, I, T Honda, H Anzai, T Seki

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY　Vol. 68　No. 12　p. 2565-2570　2004/12　Research paper (scientific journal)

Characterization of Almond α-Mannosidase and Its Application for Structure Analysis of Sugar Chain

Ryo Misaki, Kazuhito Fujiyama, Hiroyuki Yokoyama, Yoshihiro Ido, Kiyotaka Miyauchi, Toshiomi Yoshida, Tatsuji Seki

Journal of Bioscience and Bioengineering　Vol. 96　No. 2　p. 187-192　2003/02　Research paper (scientific journal)

In vivo conversion of a glycan to human compatible type by transformed tobacco cells

K Fujiyama, NQ Palacpac, H Sakai, Y Kimura, A Shinmyo, T Yoshida, T Seki

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　Vol. 289　No. 2　p. 553-557　2001/11　Research paper (scientific journal)

Glycoproteins secreted from suspension-cultured tobacco BY2 cells have distinct glycan structures from intracellular glycoproteins

FUJIYAMA Kazuhito

65/,2482-2488　Vol. 65　No. 11　p. 2482-2488　2001/11　Research paper (scientific journal)

Human N-acetylglucosaminyltransferase I. Expression in Escherichia coli as a soluble enzyme, and application as an immobilized enzyme for the chemoenzymatic synthesis of N-linked oligosaccharides

FUJIYAMA Kazuhito

92/,569-574　Vol. 92　No. 6　p. 569-574　2001/10　Research paper (scientific journal)

Identification of Putative Gene Encoded on ORF 16 of the 81kb Contig of Arabidopsis thaliana Chromosome III as .ALPHA.-Mannosidase.

FUJIYAMA KAZUHITO, KIRA YUKO, IIZUKA MASAKAZU, KIMURA YOSHINOBU, SEKI TATSUJI

Journal of Fermentation Technology　Vol. 92　No. 4　p. 401-404　2001

Characterization of Streptomyces D3 derived from protoplast fusion between Streptomyces cyaneus 190-1 and Streptomyces griseoruber 42-9

S Teeradakorn, Y Talawanich, Suzuki, I, P Pinphanichakarn, K Fujiyama, T Seki, T Yoshida

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY　Vol. 14　No. 4　p. 565-570　1998/07　Research paper (scientific journal)

A metabolic engineering view on molecular breeding of an alcohol fermenting yeast from xylose

T Seki, M Tantirungkij, K Fujiyama, T Yoshida

ENVIRONMENTAL BIOTECHNOLOGY　p. 114-124　1996　Research paper (international conference proceedings)

Characterization of tryptic jack bean α-mannosidase

FUJIYAMA Kazuhito

1994

Cloning of cDNAs encoding two peroxidases of ┣DBArabidopsis(/)-┫DB ┣DBthaliana(/)-┫DB and their organ-specific expression

FUJIYAMA Kazuhito

1993　Research paper (scientific journal)

Construction of Hybrid Genes of 6-Aminohexanoic Acid-Oligomer Hydrolase and Its Analogous Enzyme

FUJIYAMA Kazuhito

1984　Research paper (scientific journal)