An inhaled ACE2 decoy confers protection against SARS-CoV-2 infection in preclinical models.

Emiko Urano, Yumi Itoh, Tatsuya Suzuki, Takanori Sasaki, Jun-Ichi Kishikawa, Kanako Akamatsu, Yusuke Higuchi, Yusuke Sakai, Tomotaka Okamura, Shuya Mitoma, Fuminori Sugihara, Akira Takada, Mari Kimura, Shuto Nakao, Mika Hirose, Tadahiro Sasaki, Ritsuko Koketsu, Shunya Tsuji, Shota Yanagida, Tatsuo Shioda, Eiji Hara, Satoaki Matoba, Yoshiharu Matsuura, Yasunari Kanda, Hisashi Arase, Masato Okada, Junichi Takagi, Takayuki Kato, Atsushi Hoshino, Yasuhiro Yasutomi, Akatsuki Saito, Toru Okamoto

Science translational medicine　Vol. 15　No. 711　2023/08/30　Research paper (scientific journal)

Celastrol suppresses humoral immune responses and autoimmunity by targeting the COMMD3/8 complex.

Taiichiro Shirai, Akiko Nakai, Emiko Ando, Jun Fujimoto, Sarah Leach, Takao Arimori, Daisuke Higo, Floris J van Eerden, Janyerkye Tulyeu, Yu-Chen Liu, Daisuke Okuzaki, Masanori A Murayama, Haruhiko Miyata, Kazuto Nunomura, Bangzhong Lin, Akiyoshi Tani, Atsushi Kumanogoh, Masahito Ikawa, James B Wing, Daron M Standley, Junichi Takagi, Kazuhiro Suzuki

Science immunology　Vol. 8　No. 81　2023/03/31　Research paper (scientific journal)

Wnt 2022 EMBO | the Company of Biologists workshop and Yamada conference.

Akira Kikuchi, Junichi Takagi, Shinji Takada, Tohru Ishitani, Yasuhiro Minami

Genes to cells : devoted to molecular & cellular mechanisms　2023/02/27　Research paper (scientific journal)

Direct Cryo-ET observation of platelet deformation induced by SARS-CoV-2 spike protein.

Christopher Cyrus Kuhn, Nirakar Basnet, Satish Bodakuntla, Pelayo Alvarez-Brecht, Scott Nichols, Antonio Martinez-Sanchez, Lorenzo Agostini, Young-Min Soh, Junichi Takagi, Christian Biertümpfel, Naoko Mizuno

Nature communications　Vol. 14　No. 1　p. 620-620　2023/02/04　Research paper (scientific journal)

Designing receptor agonists with enhanced pharmacokinetics by grafting macrocyclic peptides into fragment crystallizable regions

Katsuya Sakai, Nozomi Sugano-Nakamura, Emiko Mihara, Nichole Marcela Rojas-Chaverra, Sayako Watanabe, Hiroki Sato, Ryu Imamura, Dominic Chih-Cheng Voon, Itsuki Sakai, Chihiro Yamasaki, Chise Tateno, Mikihiro Shibata, Hiroaki Suga, Junichi Takagi, Kunio Matsumoto

Nature Biomedical Engineering　Vol. 7　No. 2　p. 164-176　2022/11　Research paper (scientific journal)

Hybrid in vitro/in silico analysis of low-affinity protein-protein interactions that regulate signal transduction by Sema6D.

Tsubasa Tanaka, Toru Ekimoto, Meri Nagatomo, Makiko Neyazaki, Erena Shimoji, Tsutomu Yamane, Sakura Kanagawa, Rika Oi, Emiko Mihara, Junichi Takagi, Satoko Akashi, Mitsunori Ikeguchi, Terukazu Nogi

Protein science　Vol. 31　No. 11　2022/11　Research paper (scientific journal)

Structure-Based Discovery of a Novel Class of Small-Molecule Pure Antagonists of Integrin αVβ3.

Soumyo Sen, Aleksandar Spasic, Anjana Sinha, Jialing Wang, Martin Bush, Jihong Li, Dragana Nešić, Yuchen Zhou, Gabriella Angiulli, Paul Morgan, Leslie Salas-Estrada, Junichi Takagi, Thomas Walz, Barry S Coller, Marta Filizola

Journal of chemical information and modeling　2022/10/24　Research paper (scientific journal)

Engineered fast-dissociating antibody fragments for multiplexed super-resolution microscopy.

Qianli Zhang, Akitoshi Miyamoto, Shin Watanabe, Takao Arimori, Masanori Sakai, Madoka Tomisaki, Tai Kiuchi, Junichi Takagi, Naoki Watanabe

Cell reports methods　Vol. 2　No. 10　p. 100301-100301　2022/10/24　Research paper (scientific journal)

De novo Fc-based receptor dimerizers differentially modulate PlexinB1 function.

Nozomi Sugano-Nakamura, Kyoko Matoba, Mika Hirose, Nasir K Bashiruddin, Yukiko Matsunaga, Keitaro Yamashita, Kunio Hirata, Masaki Yamamoto, Takao Arimori, Hiroaki Suga, Junichi Takagi

Structure (London, England : 1993)　Vol. 30　No. 10　p. 1411-1423　2022/10/06　Research paper (scientific journal)

Engineering ACE2 decoy receptors to combat viral escapability

Takao Arimori, Nariko Ikemura, Toru Okamoto, Junichi Takagi, Daron M. Standley, Atsushi Hoshino

Trends in Pharmacological Sciences　2022/07　Research paper (scientific journal)

Functional role of the Frizzled linker domain in the Wnt signaling pathway.

Seung-Bum Ko, Emiko Mihara, Yedarm Park, Kyeonghwan Roh, Chanhee Kang, Junichi Takagi, Injin Bang, Hee-Jung Choi

Communications biology　Vol. 5　No. 1　p. 421-421　2022/05/05　Research paper (scientific journal)

An engineered ACE2 decoy neutralizes the SARS-CoV-2 Omicron variant and confers protection against infection in vivo

Nariko Ikemura, Shunta Taminishi, Tohru Inaba, Takao Arimori, Daisuke Motooka, Kazutaka Katoh, Yuhei Kirita, Yusuke Higuchi, Songling Li, Tatsuya Suzuki, Yumi Itoh, Yuki Ozaki, Shota Nakamura, Satoaki Matoba, Daron M Standley, Toru Okamoto, Junichi Takagi, Atsushi Hoshino

Science Translational Medicine　Vol. 14　No. 650　2022/04/26　Research paper (scientific journal)

Selective targeting of multiple myeloma cells with a monoclonal antibody recognizing the ubiquitous protein CD98 heavy chain.

Kana Hasegawa, Shunya Ikeda, Moto Yaga, Kouki Watanabe, Rika Urakawa, Akie Iehara, Mai Iwai, Seishin Hashiguchi, Soyoko Morimoto, Fumihiro Fujiki, Hiroko Nakajima, Jun Nakata, Sumiyuki Nishida, Akihiro Tsuboi, Yoshihiro Oka, Satoshi Yoshihara, Masahiro Manabe, Hiroyoshi Ichihara, Atsuko Mugitani, Yasutaka Aoyama, Takafumi Nakao, Asao Hirose, Masayuki Hino, Shiho Ueda, Katsuto Takenaka, Takashi Masuko, Koichi Akashi, Takahiro Maruno, Susumu Uchiyama, Shinji Takamatsu, Naoki Wada, Eiichi Morii, Shushi Nagamori, Daisuke Motooka, Yoshikatsu Kanai, Yusuke Oji, Tomoyoshi Nakagawa, Noriyuki Kijima, Haruhiko Kishima, Atsuyo Ikeda, Takayuki Ogino, Yasushi Shintani, Tateki Kubo, Emiko Mihara, Kosuke Yusa, Haruo Sugiyama, Junichi Takagi, Eiji Miyoshi, Atsushi Kumanogoh, Naoki Hosen

Science translational medicine　Vol. 14　No. 632　2022/02/16　Research paper (scientific journal)

Serum autoantibodies against the extracellular region of α6β4 integrin in a patient with dipeptidyl peptidase-4 inhibitor-induced bullous pemphigoid.

Chigusa Yamashita, Noriko Arase, Shuhei Higuchi, Hisashi Arase, Junichi Takagi, Satoshi Nojima, Atsushi Tanemura, Manabu Fujimoto

JAAD case reports　Vol. 20　p. 65-68　2022/02

Lasso-grafting of macrocyclic peptide pharmacophores yields multi-functional proteins

Emiko Mihara, Satoshi Watanabe, Nasir K. Bashiruddin, Nozomi Nakamura, Kyoko Matoba, Yumi Sano, Rumit Maini, Yizhen Yin, Katsuya Sakai, Takao Arimori, Kunio Matsumoto, Hiroaki Suga, Junichi Takagi

Nature Communications　Vol. 12　No. 1　2021/12　Research paper (scientific journal)

Engineered ACE2 receptor therapy overcomes mutational escape of SARS-CoV-2

Yusuke Higuchi, Tatsuya Suzuki, Takao Arimori, Nariko Ikemura, Emiko Mihara, Yuhei Kirita, Eriko Ohgitani, Osam Mazda, Daisuke Motooka, Shota Nakamura, Yusuke Sakai, Yumi Itoh, Fuminori Sugihara, Yoshiharu Matsuura, Satoaki Matoba, Toru Okamoto, Junichi Takagi, Atsushi Hoshino

Nature Communications　Vol. 12　No. 1　2021/12　Research paper (scientific journal)

Structural mechanism of laminin recognition by integrin

Takao Arimori, Naoyuki Miyazaki, Emiko Mihara, Mamoru Takizawa, Yukimasa Taniguchi, Carlos Cabañas, Kiyotoshi Sekiguchi, Junichi Takagi

Nature Communications　Vol. 12　No. 1　2021/12　Research paper (scientific journal)

De novo peptide grafting to a self-assembling nanocapsule yields a hepatocyte growth factor receptor agonist.

Yamato Komatsu, Naohiro Terasaka, Katsuya Sakai, Emiko Mihara, Risa Wakabayashi, Kunio Matsumoto, Donald Hilvert, Junichi Takagi, Hiroaki Suga

iScience　Vol. 24　No. 11　p. 103302-103302　2021/11/19　Research paper (scientific journal)

Structural studies of reelin N-terminal region provides insights into a unique structural arrangement and functional multimerization.

Masamichi Nagae, Kei Suzuki, Norihisa Yasui, Terukazu Nogi, Takao Kohno, Mitsuharu Hattori, Junichi Takagi

Journal of biochemistry　Vol. 169　No. 5　p. 555-564　2021/07/03　Research paper (scientific journal)

Molecular recognition of a single-chain Fv antibody specific for GA-pyridine, an advanced glycation end-product (AGE), elucidated using biophysical techniques and synthetic antigen analogues.

Yoshihiro Kobashigawa, Toshiya Ohara, Kosuke Morita, Yuya Toyota, Teruya Nakamura, Shunsuke Kotani, Takao Arimori, Soichiro Yamauchi, Chenjiang Liu, Masaya Kitazaki, Yukari Wakeyama-Miyazaki, Yoshiaki Suwa, Makiyo Uchida-Kamekura, Natsuki Fukuda, Takashi Sato, Makoto Nakajima, Junichi Takagi, Yuriko Yamagata, Hiroshi Morioka

Journal of biochemistry　Vol. 170　No. 3　p. 379-387　2021/06/29　Research paper (scientific journal)

Structural insights into integrin α 5 β 1 opening by fibronectin ligand

Stephanie Schumacher, Dirk Dedden, Roberto Vazquez Nunez, Kyoko Matoba, Junichi Takagi, Christian Biertümpfel, Naoko Mizuno

Science Advances　Vol. 7　No. 19　2021/05　Research paper (scientific journal)

A feasibility study of inverse contrast-matching small-angle neutron scattering method combined with size exclusion chromatography using antibody interactions as model systems.

Nobuhiro Sato, Rina Yogo, Saeko Yanaka, Anne Martel, Lionel Porcar, Ken Morishima, Rintaro Inoue, Taiki Tominaga, Takao Arimori, Junichi Takagi, Masaaki Sugiyama, Koichi Kato

Journal of biochemistry　2021/02/02　Research paper (scientific journal)

Development of cyclic peptides with potent in vivo osteogenic activity through RaPID-based affinity maturation.

Nasir K Bashiruddin, Mikihito Hayashi, Masanobu Nagano, Yan Wu, Yukiko Matsunaga, Junichi Takagi, Tomoki Nakashima, Hiroaki Suga

Proceedings of the National Academy of Sciences of the United States of America　Vol. 117　No. 49　p. 31070-31077　2020/12/08　Research paper (scientific journal)

High affinity modified ACE2 receptors protect from SARS-CoV-2 infection in hamsters

Yusuke Higuchi, Tatsuya Suzuki, Takao Arimori, Nariko Ikemura, Emiko Mihara, Yuhei Kirita, Eriko Ohgitani, Osam Mazda, Daisuke Motooka, Shota Nakamura, Yusuke Sakai, Yumi Itoh, Fuminori Sugihara, Yoshiharu Matsuura, Satoaki Matoba, Toru Okamoto, Junichi Takagi, Atsushi Hoshino

2020/09/16

Dissection of α4β7 integrin regulation by Rap1 using novel conformation-specific monoclonal anti-β7 antibodies.

Tsuyoshi Sato, Sayaka Ishihara, Ryoya Marui, Junichi Takagi, Koko Katagiri

Scientific reports　Vol. 10　No. 1　p. 13221-13221　2020/08/06　Research paper (scientific journal)

Negatively charged amino acids in the stalk region of membrane proteins reduce ectodomain shedding.

Ryo Iwagishi, Rika Tanaka, Munenosuke Seto, Tomoyo Takagi, Naoko Norioka, Tomoe Ueyama, Teruhisa Kawamura, Junichi Takagi, Yoshihiro Ogawa, Kyoko Shirakabe

The Journal of biological chemistry　2020/06/24　Research paper (scientific journal)

Site-specific epitope insertion into recombinant proteins using the MAP tag system.

Ayami Wakasa, Mika K Kaneko, Yukinari Kato, Junichi Takagi, Takao Arimori

Journal of biochemistry　Vol. 168　No. 4　p. 375-384　2020/05/09　Research paper (scientific journal)

Macrocyclic peptides that inhibit Wnt signalling via interaction with Wnt3a

Manuel E. Otero-Ramirez, Kyoko Matoba, Emiko Mihara, Toby Passioura, Junichi Takagi, Hiroaki Suga

RSC Chemical Biology　Vol. 1　No. 1　p. 26-34　2020　Research paper (scientific journal)

The constitutive high affinity Met binding site in the kringle domain is dispensable for the signaling activity of hepatocyte growth factor.

Masataka Umitsu, Katsuya Sakai, Keiko Tamura-Kawakami, Kunio Matsumoto, Junichi Takagi

Journal of biochemistry　Vol. 167　No. 6　p. 577-586　2020/01　Research paper (scientific journal)

Novel Pure αVβ3 Integrin Antagonists That Do Not Induce Receptor Extension, Prime the Receptor, or Enhance Angiogenesis at Low Concentrations.

Jihong Li, Yoshiyuki Fukase, Yi Shang, Wei Zou, José M Muñoz-Félix, Lorena Buitrago, Johannes van Agthoven, Yixiao Zhang, Ryoma Hara, Yuta Tanaka, Rei Okamoto, Takeshi Yasui, Takashi Nakahata, Toshihiro Imaeda, Kazuyoshi Aso, Yuchen Zhou, Charles Locuson, Dragana Nesic, Mark Duggan, Junichi Takagi, Roger D Vaughan, Thomas Walz, Kairbaan Hodivala-Dilke, Steven L Teitelbaum, M Amin Arnaout, Marta Filizola, Michael A Foley, Barry S Coller

ACS pharmacology & translational science　Vol. 2　No. 6　p. 387-401　2019/12/13　Research paper (scientific journal)

環状ペプチドによるHGF阻害と高速原子間力顕微鏡による分子動態計測

酒井 克也, 佐藤 拓輝, 柴田 幹大, 高木 淳一, 加藤 幸成, 向井 英史, 渡辺 恭良, 矢野 聖二, 管 裕明, 松本 邦夫

日本生化学会大会プログラム・講演要旨集　Vol. 92回　p. [2T09a-03]　2019/09

環状ペプチドによるHGF阻害と高速原子間力顕微鏡による分子動態計測

酒井 克也, 佐藤 拓輝, 柴田 幹大, 高木 淳一, 加藤 幸成, 向井 英史, 渡辺 恭良, 矢野 聖二, 管 裕明, 松本 邦夫

日本生化学会大会プログラム・講演要旨集　Vol. 92回　p. [2T09a-03]　2019/09

Impaired ligand-dependent MET activation caused by an extracellular SEMA domain missense mutation in lung cancer.

Wenyu Miao, Katsuya Sakai, Hiroki Sato, Ryu Imamura, Nawaphat Jangphattananont, Junichi Takagi, Michiru Nishita, Yasuhiro Minami, Kunio Matsumoto

Cancer science　Vol. 110　No. 10　p. 3340-3349　2019/07　Research paper (scientific journal)

Macrocyclic peptide-based inhibition and imaging of hepatocyte growth factor.

Katsuya Sakai, Toby Passioura, Hiroki Sato, Kenichiro Ito, Hiroki Furuhashi, Masataka Umitsu, Junichi Takagi, Yukinari Kato, Hidefumi Mukai, Shota Warashina, Maki Zouda, Yasuyoshi Watanabe, Seiji Yano, Mikihiro Shibata, Hiroaki Suga, Kunio Matsumoto

Nature chemical biology　Vol. 15　No. 6　p. 598-606　2019/06　Research paper (scientific journal)

Distinct Localization of Mature HGF from its Precursor Form in Developing and Repairing the Stomach.

Nawaphat Jangphattananont, Hiroki Sato, Ryu Imamura, Katsuya Sakai, Yumi Terakado, Kazuhiro Murakami, Nick Barker, Hiroko Oshima, Masanobu Oshima, Junichi Takagi, Yukinari Kato, Seiji Yano, Kunio Matsumoto

International journal of molecular sciences　Vol. 20　No. 12　2019/06　Research paper (scientific journal)

Macrocyclic peptide-based inhibition and imaging of hepatocyte growth factor

Sakai K, Passioura T, Sato H, Ito K, Furuhashi H, Umitsu M, Takagi J, Kato Y, Mukai H, Warashina S, Zouda M, Watanabe Y, Yano S, Shibata M, Suga H, Matsumoto K

Nature Chemical Biology　Vol. 00　No. 00　p. 00-00　2019/05　Research paper (scientific journal)

Crystal structure of a mammalian Wnt-frizzled complex.

Hirai H, Matoba K, Mihara E, Arimori T, Takagi J

Nature structural & molecular biology　Vol. 26　No. 5　p. 372-379　2019/05　Research paper (scientific journal)

Structural basis for oligomerization of the prokaryotic peptide transporter PepT_So2.

Nagamura R, Fukuda M, Kawamoto A, Matoba K, Dohmae N, Ishitani R, Takagi J, Nureki O

Acta crystallographica. Section F, Structural biology communications　Vol. 75　No. Pt 5　p. 348-358　2019/05　Research paper (scientific journal)

Advances in domain and subunit localization technology for electron microscopy.

Brown ZP, Takagi J

Biophysical reviews　Vol. 11　No. 2　p. 149-155　2019/04

Structural Basis of Sarco/Endoplasmic Reticulum Ca²⁺-ATPase 2b Regulation via Transmembrane Helix Interplay.

Inoue M, Sakuta N, Watanabe S, Zhang Y, Yoshikaie K, Tanaka Y, Ushioda R, Kato Y, Takagi J, Tsukazaki T, Nagata K, Inaba K

Cell reports　Vol. 27　No. 4　p. 1221-1230.e3　2019/04　Research paper (scientific journal)

Cellular signaling and gene expression profiles evoked by a bivalent macrocyclic peptide that serves as an artificial MET receptor agonist.

Wenyu Miao, Katsuya Sakai, Naoya Ozawa, Takumi Nishiuchi, Yoshinori Suzuki, Kenichiro Ito, Tomomi Morioka, Masataka Umitsu, Junichi Takagi, Hiroaki Suga, Kunio Matsumoto

Scientific reports　Vol. 8　No. 1　p. 16492-16492　2018/11　Research paper (scientific journal)

Off-axis rotor in Enterococcus hirae V-ATPase visualized by Zernike phase plate single-particle cryo-electron microscopy.

Jun Tsunoda, Chihong Song, Fabiana Lica Imai, Junichi Takagi, Hiroshi Ueno, Takeshi Murata, Ryota Iino, Kazuyoshi Murata

Scientific reports　Vol. 8　No. 1　p. 15632-15632　2018/10　Research paper (scientific journal)

Structure and mechanism of cancer-associated N-acetylglucosaminyltransferase-V

Nagae Masamichi, Kizuka Yasuhiko, Mihara Emiko, Kitago Yu, Hanashima Shinya, Ito Yukishige, Takagi Junichi, Taniguchi Naoyuki, Yamaguchi Yoshiki

NATURE COMMUNICATIONS　Vol. 9　No. 1　p. 3380-3380　2018/08　Research paper (scientific journal)

A novel small antibody fragment design; Fv-clasp

Takao Arimori, Junichi Takagi

Jikken Igaku　Vol. 36　No. 11　p. 1849-1853　2018/07

An anti-peptide monoclonal antibody recognizing the tobacco etch virus protease-cleavage sequence and its application to a tandem tagging system

Sanae Tabata, Yu Kitago, Yuki Fujii, Emiko Mihara, Keiko Tamura-Kawakami, Naoko Norioka, Katsu Takahashi, Mika K. Kaneko, Yukinari Kato, Junichi Takagi

Protein Expression and Purification　Vol. 147　p. 94-99　2018/07　Research paper (scientific journal)

Facile Synthesis of Dimeric Thioether-Macrocyclic Peptides with Antibody-like Affinity against Plexin-B1.

Nasir K Bashiruddin, Yukiko Matsunaga, Masanobu Nagano, Junichi Takagi, Hiroaki Suga

Bioconjugate chemistry　Vol. 29　No. 6　p. 1847-1851　2018/06　Research paper (scientific journal)

Semaphorin 6D reverse signaling controls macrophage lipid metabolism and anti-inflammatory polarization

Sujin Kang, Yoshimitsu Nakanishi, Yoshiyuki Kioi, Daisuke Okuzaki, Tetsuya Kimura, Hyota Takamatsu, Shohei Koyama, Satoshi Nojima, Masayuki Nishide, Yoshitomo Hayama, Yuhei Kinehara, Yasuhiro Kato, Takeshi Nakatani, Tomomi Shimogori, Junichi Takagi, Toshihiko Toyofuku, Atsushi Kumanogoh

Nature Immunology　Vol. 19　No. 6　p. 561-570　2018/06　Research paper (scientific journal)

Yeast Inner-Subunit PA–NZ-1 Labeling Strategy for Accurate Subunit Identification in a Macromolecular Complex through Cryo-EM Analysis

Huping Wang, Wenyu Han, Junichi Takagi, Yao Cong

Journal of Molecular Biology　Vol. 430　No. 10　p. 1417-1425　2018/05　Research paper (scientific journal)

A systematic survey of conformational states in β1 and β4 integrins using negative-stain electron microscopy.

Miyazaki N, Iwasaki K, Takagi J

Journal of cell science　Vol. 131　No. 10　2018/05　Research paper (scientific journal)

The PA Tag: A Versatile Peptide Tagging System in the Era of Integrative Structural Biology.

Brown ZP, Takagi J

Advances in experimental medicine and biology　Vol. 1105　p. 59-76　2018/04　Research paper (scientific journal)

Development of a new protein labeling system to map subunits and domains of macromolecular complexes for electron microscopy.

Brown ZP, Arimori T, Iwasaki K, Takagi J

Journal of structural biology　Vol. 201　No. 3　p. 247-251　2018/03　Research paper (scientific journal)

Human Pancreatic Tumor Organoids Reveal Loss of Stem Cell Niche Factor Dependence during Disease Progression

Takashi Seino, Shintaro Kawasaki, Mariko Shimokawa, Hiroki Tamagawa, Kohta Toshimitsu, Masayuki Fujii, Yuki Ohta, Mami Matano, Kosaku Nanki, Kenta Kawasaki, Sirirat Takahashi, Shinya Sugimoto, Eisuke Iwasaki, Junichi Takagi, Takao Itoi, Minoru Kitago, Yuko Kitagawa, Takanori Kanai, Toshiro Sato

Cell Stem Cell　Vol. 22　No. 3　p. 454-467.e6　2018/03　Research paper (scientific journal)

Foreword to ‘Multiscale structural biology: biophysical principles and mechanisms underlying the action of bio-nanomachines’, a special issue in Honour of Fumio Arisaka’s 70th birthday

Damien Hall, Junichi Takagi, Haruki Nakamura

Biophysical Reviews　Vol. 10　No. 2　p. 105-129　2018/03　Research paper (scientific journal)

Corrigendum: Immune evasion of Plasmodium falciparum by RIFIN via inhibitory receptors.

Saito F, Hirayasu K, Satoh T, Wang CW, Lusingu J, Arimori T, Shida K, Palacpac NMQ, Itagaki S, Iwanaga S, Takashima E, Tsuboi T, Kohyama M, Suenaga T, Colonna M, Takagi J, Lavstsen T, Horii T, Arase H

Nature　Vol. 554　No. 7693　p. 554-554　2018/02　Research paper (scientific journal)

Molecular dissection of protein-protein interactions between integrin alpha 5 beta 1 and the Helicobacter pylori Cag type IV secretion system

Thomas Koelblen, Celia Berge, Mickael V. Cherrier, Karl Brillet, Luisa Jimenez-Soto, Lionel Ballut, Junichi Takagi, Roland Montserret, Patricia Rousselle, Wolfgang Fischer, Rainer Haas, Remi Fronzes, Laurent Terradot

FEBS JOURNAL　Vol. 284　No. 23　p. 4143-4157　2017/12　Research paper (scientific journal)

The activated conformation of integrin β7 is a novel multiple myeloma-specific target for CAR T cell therapy.

Naoki Hosen, Yukiko Matsunaga, Kana Hasegawa, Hiroshi Matsuno, Yuki Nakamura, Mio Makita, Kouki Watanabe, Mikako Yoshida, Kei Satoh, Soyoko Morimoto, Fumihiro Fujiki, Hiroko Nakajima, Jun Nakata, Sumiyuki Nishida, Akihiro Tsuboi, Yoshihiro Oka, Masahiro Manabe, Hiroyoshi Ichihara, Yasutaka Aoyama, Atsuko Mugitani, Takafumi Nakao, Masayuki Hino, Ryosuke Uchibori, Keiya Ozawa, Yoshihiro Baba, Seitaro Terakura, Naoki Wada, Eiichi Morii, Junichi Nishimura, Kiyoshi Takeda, Yusuke Oji, Haruo Sugiyama, Junichi Takagi, Atsushi Kumanogoh

Nature medicine　Vol. 23　No. 12　p. 1436-1443　2017/12　Research paper (scientific journal)

Clonal evolution and antigen recognition of anti-nuclear antibodies in acute systemic lupus erythematosus

Shuhei Sakakibara, Takao Arimori, Kazuo Yamashita, Hideyuki Jinzai, Daisuke Motooka, Shota Nakamura, Songling Li, Kazuya Takeda, Jun Katayama, Marwa Ali El Hussien, Masashi Narazaki, Toshio Tanaka, Daron M. Standley, Junichi Takagi, Hitoshi Kikutani

Scientific Reports　Vol. 7　No. 1　2017/12　Research paper (scientific journal)

Immune evasion of Plasmodium falciparum by RIFIN via inhibitory receptors.

Fumiji Saito, Kouyuki Hirayasu, Takeshi Satoh, Christian W Wang, John Lusingu, Takao Arimori, Kyoko Shida, Nirianne Marie Q Palacpac, Sawako Itagaki, Shiroh Iwanaga, Eizo Takashima, Takafumi Tsuboi, Masako Kohyama, Tadahiro Suenaga, Marco Colonna, Junichi Takagi, Thomas Lavstsen, Toshihiro Horii, Hisashi Arase

Nature　Vol. 552　No. 7683　p. 101-105　2017/12　Research paper (scientific journal)

Fv-clasp: An Artificially Designed Small Antibody Fragment with Improved Production Compatibility, Stability, and Crystallizability

Takao Arimori, Yu Kitago, Masataka Umitsu, Yuki Fujii, Ryoko Asaki, Keiko Tamura-Kawakami, Junichi Takagi

STRUCTURE　Vol. 25　No. 10　p. 1611-1622.e4　2017/10　Research paper (scientific journal)

Mechanistic basis for the recognition of laminin-511 by α6β1 integrin.

Takizawa M, Arimori T, Taniguchi Y, Kitago Y, Yamashita E, Takagi J, Sekiguchi K, lly contri

Science advances　Vol. 3　No. 9　2017/09　Research paper (scientific journal)

Structural basis for ligand capture and release by the endocytic receptor ApoER2

Hidenori Hirai, Norihisa Yasui, Keitaro Yamashita, Sanae Tabata, Masaki Yamamoto, Junichi Takagi, Terukazu Nogi

EMBO REPORTS　Vol. 18　No. 6　p. 982-999　2017/06　Research paper (scientific journal)

Loss of Parkinson's disease-associated protein CHCHD2 affects mitochondrial crista structure and destabilizes cytochrome c

Hongrui Meng, Chikara Yamashita, Kahori Shiba-Fukushima, Tsuyoshi Inoshita, Manabu Funayama, Shigeto Sato, Tomohisa Hatta, Tohru Natsume, Masataka Umitsu, Junichi Takagi, Yuzuru Imai, Nobutaka Hattori

NATURE COMMUNICATIONS　Vol. 8　2017/06　Research paper (scientific journal)

The unique prodomain of T-cadherin plays a key role in adiponectin binding with the essential extracellular cadherin repeats 1 and 2

Shiro Fukuda, Shunbun Kita, Yoshinari Obata, Yuya Fujishima, Hirofumi Nagao, Shigeki Masuda, Yoshimitsu Tanaka, Hitoshi Nishizawa, Tohru Funahashi, Junichi Takagi, Norikazu Maeda, Iichiro Shimomura

JOURNAL OF BIOLOGICAL CHEMISTRY　Vol. 292　No. 19　p. 7840-+　2017/05　Research paper (scientific journal)

Structural basis for perception of diverse chemical substances by T1r taste receptors

Nipawan Nuemket, Norihisa Yasui, Yuko Kusakabe, Yukiyo Nomura, Nanako Atsumi, Shuji Akiyama, Eriko Nango, Yukinari Kato, Mika K. Kaneko, Junichi Takagi, Maiko Hosotani, Atsuko Yamashita

NATURE COMMUNICATIONS　Vol. 8　2017/05　Research paper (scientific journal)

Immunoaffinity purification of the glycosylated extracellular fragment of mouse Plexin A2 produced in a mammalian expression system

Terukazu Nogi, Emiko Mihara, Norihisa Yasui, Junichi Takagi

Methods in Molecular Biology　Vol. 1493　p. 57-72　2017/04　Research paper (scientific journal)

Mechanistic insights into ectodomain shedding: susceptibility of CADM1 adhesion molecule is determined by alternative splicing and O-glycosylation.

Kyoko Shirakabe, Takuya Omura, Yoshio Shibagaki, Emiko Mihara, Keiichi Homma, Yukinari Kato, Akihiko Yoshimura, Yoshinori Murakami, Junichi Takagi, Seisuke Hattori, Yoshihiro Ogawa

Scientific reports　Vol. 7　p. 46174-46174　2017/04　Research paper (scientific journal)

2D hybrid analysis: Approach for building three-dimensional atomic model by electron microscopy image matching

Atsushi Matsumoto, Naoyuki Miyazaki, Junichi Takagi, Kenji Iwasaki

SCIENTIFIC REPORTS　Vol. 7　No. 1　2017/03　Research paper (scientific journal)

Conformational Freedom of the LRP6 Ectodomain Is Regulated by N-glycosylation and the Binding of the Wnt Antagonist Dkk1

Kyoko Matoba, Emiko Mihara, Keiko Tamura-Kawakami, Naoyuki Miyazaki, Shintaro Maeda, Hidenori Hirai, Samuel Thompson, Kenji Iwasaki, Junichi Takagi

CELL REPORTS　Vol. 18　No. 1　p. 32-40　2017/01　Research paper (scientific journal)

Allosteric Inhibition of a Semaphorin 4D Receptor Plexin B1 by a High-Affinity Macrocyclic Peptide

Yukiko Matsunaga, Nasir K. Bashiruddin, Yu Kitago, Junichi Takagi, Hiroaki Suga

CELL CHEMICAL BIOLOGY　Vol. 23　No. 11　p. 1341-1350　2016/11　Research paper (scientific journal)

Allosteric Inhibition of a Semaphorin 4D Receptor Plexin B1 by a High-Affinity Macrocyclic Peptide

Yukiko Matsunaga, Nasir K. Bashiruddin, Yu Kitago, Junichi Takagi, Hiroaki Suga

Cell Chemical Biology　Vol. 23　No. 11　p. 1341-1350　2016/11　Research paper (scientific journal)

Redox-assisted regulation of Ca2+ homeostasis in the endoplasmic reticulum by disulfide reductase ERdj5

Ryo Ushioda, Akitoshi Miyamoto, Michio Inoue, Satoshi Watanabe, Masaki Okumura, Ken-ichi Maegawa, Kaiku Uegaki, Shohei Fujii, Yasuko Fukuda, Masataka Umitsu, Junichi Takagi, Kenji Inaba, Katsuhiko Mikoshiba, Kazuhiro Nagata

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　Vol. 113　No. 41　p. E6055-E6063　2016/10　Research paper (scientific journal)

Structural basis for multi-specific peptide recognition by the anti-IDH1/2 monoclonal antibody, MsMab-1

Yu Kitago, Mika K. Kaneko, Satoshi Ogasawara, Yukinari Kato, Junichi Takagi

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　Vol. 478　No. 3　p. 1274-9　2016/09　Research paper (scientific journal)

Probing conformational and functional states of human hepatocyte growth factor by a panel of monoclonal antibodies.

Masataka Umitsu, Katsuya Sakai, Satoshi Ogasawara, Mika K Kaneko, Ryoko Asaki, Keiko Tamura-Kawakami, Yukinari Kato, Kunio Matsumoto, Junichi Takagi

Scientific reports　Vol. 6　p. 33149-33149　2016/09　Research paper (scientific journal)

The PA Tag: A Turn-forming Peptide Tag That Can Be Placed into the Structured Domain of a Protein in a Tailored Manner

TAKAGI Junichi

Seibutsu Butsuri　Vol. 56　No. 5　p. 284-287　2016/04

Tailored placement of a turn-forming PA tag into the structured domain of a protein to probe its conformational state

Yuki Fujii, Yukiko Matsunaga, Takao Arimori, Yu Kitago, Satoshi Ogasawara, Mika K. Kaneko, Yukinari Kato, Junichi Takagi

JOURNAL OF CELL SCIENCE　Vol. 129　No. 7　p. 1512-22　2016/04　Research paper (scientific journal)

Structure of the Plexin Ectodomain Bound by Semaphorin-Mimicking Antibodies.

Kei Suzuki, Hiroyuki Tsunoda, Ryusuke Omiya, Kyoko Matoba, Takeshi Baba, Sachiyo Suzuki, Hiroaki Segawa, Atsushi Kumanogoh, Kenji Iwasaki, Kunihiro Hattori, Junichi Takagi

PloS one　Vol. 11　No. 6　2016/04　Research paper (scientific journal)

Molecular Basis of the Ligand Binding Specificity of alpha v beta 8 Integrin

Akio Ozawa, Yuya Sato, Tsukasa Imabayashi, Toshihiko Uemura, Junichi Takagi, Kiyotoshi Sekiguchi

JOURNAL OF BIOLOGICAL CHEMISTRY　Vol. 291　No. 22　p. 11551-11565　2016/03　Research paper (scientific journal)

Structure and biological function of ENPP6, a choline-specific glycerophosphodiester-phosphodiesterase

Junko Morita, Kuniyuki Kano, Kazuki Kato, Hiroyuki Takita, Hideki Sakagami, Yasuo Yamamoto, Emiko Mihara, Hirofumi Ueda, Takanao Sato, Hidetoshi Tokuyama, Hiroyuki Arai, Hiroaki Asou, Junichi Takagi, Ryuichiro Ishitani, Hiroshi Nishimasu, Osamu Nureki, Junken Aoki

SCIENTIFIC REPORTS　Vol. 6　2016/02　Research paper (scientific journal)

Active and water-soluble form of lipidated Wnt protein is maintained by a serum glycoprotein afamin/alpha-albumin

Emiko Mihara, Hidenori Hirai, Hideki Yamamoto, Keiko Tamura-Kawakami, Mami Matano, Akira Kikuchi, Toshiro Sato, Junichi Takagi

ELIFE　Vol. 5　2016/02　Research paper (scientific journal)

Interplay of Endosomal pH and Ligand Occupancy in Integrin alpha 5 beta 1 Ubiquitination, Endocytic Sorting, and Cell Migration

Dmitri Kharitidi, Pirjo M. Apaja, Sanaz Manteghi, Kei Suzuki, Elena Malitskaya, Ariel Roldan, Marie-Claude Gingras, Junichi Takagi, Gergely L. Lukacs, Arnim Pause

CELL REPORTS　Vol. 13　No. 3　p. 599-609　2015/10　Research paper (scientific journal)

Structural basis for amyloidogenic peptide recognition by sorLA

Yu Kitago, Masamichi Nagae, Zenzaburo Nakata, Maho Yagi-Utsumi, Shizuka Takagi-Niidome, Emiko Mihara, Terukazu Nogi, Koichi Kato, Junichi Takagi

NATURE STRUCTURAL & MOLECULAR BIOLOGY　Vol. 22　No. 3　p. 199-206　2015/03　Research paper (scientific journal)

Expression, purification, crystallization and preliminary X-ray crystallographic analysis of Enpp6

Junko Morita, Kazuki Kato, Emiko Mihara, Ryuichiro Ishitani, Junichi Takagi, Hiroshi Nishimasu, Junken Aoki, Osamu Nureki

Acta Crystallographica Section F:Structural Biology Communications　Vol. 70　No. Pt 6　p. 794-9　2014/06　Research paper (scientific journal)

Expression, purification, crystallization and preliminary X-ray crystallographic analysis of Enpp6

Junko Morita, Kazuki Kato, Emiko Mihara, Ryuichiro Ishitani, Junichi Takagi, Hiroshi Nishimasu, Junken Aoki, Osamu Nureki

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS　Vol. 70　No. Pt 6　p. 794-799　2014/06　Research paper (scientific journal)

Editorial overview: New constructs and expression of proteins: Making things better

Junichi Takagi, Christopher G. Tate

CURRENT OPINION IN STRUCTURAL BIOLOGY　Vol. 26　p. iv-vi　2014/06　Research paper (scientific journal)

PA tag: A versatile protein tagging system using a super high affinity antibody against a dodecapeptide derived from human podoplanin

Yuki Fujii, Mika Kaneko, Makiko Neyazaki, Terukazu Nogi, Yukinari Kato, Junichi Takagi

PROTEIN EXPRESSION AND PURIFICATION　Vol. 95　p. 240-7　2014/03　Research paper (scientific journal)

A Structure-Based Model of Substrate Discrimination by a Noncanonical PDZ Tandem in the Intramembrane-Cleaving Protease RseP

Yohei Hizukuri, Takashi Oda, Sanae Tabata, Keiko Tamura-Kawakami, Rika Oi, Mamoru Sato, Junichi Takagi, Yoshinori Akiyama, Terukazu Nogi

STRUCTURE　Vol. 22　No. 2　p. 326-36　2014/02　Research paper (scientific journal)

Lysosomal Sorting of Amyloid-beta by the SORLA Receptor Is Impaired by a Familial Alzheimer's Disease Mutation

Safak Caglayan, Shizuka Takagi-Niidome, Fan Liao, Anne-Sophie Carlo, Vanessa Schmidt, Tilman Burgert, Yu Kitago, Ernst-Martin Fuechtbauer, Annette Fuechtbauer, David M. Holtzman, Junichi Takagi, Thomas E. Willnow

SCIENCE TRANSLATIONAL MEDICINE　Vol. 6　No. 223　2014/02　Research paper (scientific journal)

SACLAを利用したXFEL微結晶構造解析

溝端栄一, 鈴木守, 南後恵理子, 森田能次, 垣之内啓介, 田中里枝, 登野健介, 城地保昌, 亀島敬, Jaehyun Park, Changyong Song, 初井宇記, 矢橋牧名, Thomas A White, 北郷悠, 大洞光司, 石田英子, 廣瀬未果, 杉山成, 村田道雄, 林高史, 高木淳一, 井上豪, 岩田想

日本結晶学会年会講演要旨集　Vol. 2013　2013/10　Research paper (other academic)

Crystal structure and enzymatic activity of an ADAMTS-13 mutant with the East Asian-specific P475S polymorphism

M. Akiyama, D. Nakayama, S. Takeda, K. Kokame, J. Takagi, T. Miyata

JOURNAL OF THROMBOSIS AND HAEMOSTASIS　Vol. 11　No. 7　p. 1399-1406　2013/07　Research paper (scientific journal)

A point mutation in Semaphorin 4A associates with defective endosomal sorting and causes retinal degeneration

Satoshi Nojima, Toshihiko Toyofuku, Hiroyuki Kamao, Chie Ishigami, Jun Kaneko, Tatsusada Okuno, Hyota Takamatsu, Daisuke Ito, Sujin Kang, Tetsuya Kimura, Yuji Yoshida, Keiko Morimoto, Yohei Maeda, Atsushi Ogata, Masahito Ikawa, Eiichi Morii, Katsuyuki Aozasa, Junichi Takagi, Masayo Takahashi, Atsushi Kumanogoh

NATURE COMMUNICATIONS　Vol. 4　2013/03　Research paper (scientific journal)

A multipurpose fusion tag derived from an unstructured and hyperacidic region of the amyloid precursor protein

Takeshi Sangawa, Sanae Tabata, Kei Suzuki, Yasushi Saheki, Keiji Tanaka, Junichi Takagi

Protein Science　Vol. 22　No. 6　p. 840-850　2013/03　Research paper (scientific journal)

3P032 Substrate discrimination mechanism by a PDZ tandem in the intramembrane protease RseP that regulates extracytoplasmic stress response(01A. Protein: Structure,Poster)

Hizukuri Yohei, Oda Takashi, Tabata Sanae, Tamura-Kawakami Keiko, Sato Mamoru, Takagi Junichi, Nogi Terukazu, Akiyama Yoshinori

Seibutsu Butsuri　Vol. 53　No. 1　2013

Schwann cell myelination requires integration of laminin activities

Karen K. Mckee, Dong-Hua Yang, Rajesh Patel, Zu-Lin Chen, Sidney Strickland, Junichi Takagi, Kiyotoshi Sekiguchi, Peter D. Yurchenco

JOURNAL OF CELL SCIENCE　Vol. 125　No. Pt 19　p. 4609-19　2012/10　Research paper (scientific journal)

Expression, purification, crystallization and preliminary X-ray crystallographic analysis of Enpp1

Kazuki Kato, Hiroshi Nishimasu, Emiko Mihara, Ryuichiro Ishitani, Junichi Takagi, Junken Aoki, Osamu Nureki

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS　Vol. 68　No. Pt 7　p. 778-82　2012/07　Research paper (scientific journal)

Higher-Order Architecture of Cell Adhesion Mediated by Polymorphic Synaptic Adhesion Molecules Neurexin and Neuroligin

Hiroki Tanaka, Naoyuki Miyazaki, Kyoko Matoba, Terukazu Nogi, Kenji Iwasaki, Junichi Takagi

CELL REPORTS　Vol. 2　No. 1　p. 101-110　2012/07　Research paper (scientific journal)

Crystal structure of Enpp1, an extracellular glycoprotein involved in bone mineralization and insulin signaling

Kazuki Kato, Hiroshi Nishimasu, Shinichi Okudaira, Emiko Mihara, Ryuichiro Ishitani, Junichi Takagi, Junken Aoki, Osamu Nureki

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　Vol. 109　No. 42　p. 16876-16881　2012/06　Research paper (scientific journal)

Crystal structure of alpha 5 beta 1 integrin ectodomain: Atomic details of the fibronectin receptor

Masamichi Nagae, Suyong Re, Emiko Mihara, Terukazu Nogi, Yuji Sugita, Junichi Takagi

JOURNAL OF CELL BIOLOGY　Vol. 197　No. 1　p. 131-140　2012/04　Research paper (scientific journal)

Neutralization of the γ-secretase activity by monoclonal antibody against extracellular domain of nicastrin

I. Hayashi, S. Takatori, Y. Urano, Y. Miyake, J. Takagi, M. Sakata-Yanagimoto, H. Iwanari, S. Osawa, Y. Morohashi, T. Li, P. C. Wong, S. Chiba, T. Kodama, T. Hamakubo, T. Tomita, T. Iwatsubo

Oncogene　Vol. 31　No. 6　p. 787-798　2012/02　Research paper (scientific journal)

Correlation Technology for the Next-generation Structural Life Science

TAKAGI Junichi

tits　Vol. 17　No. 12　p. 12_34-12_37　2012

Tilt-angle measurement of a sample stage using a capacitive liquid-based inclinometer

Kyoko Matoba, Junichi Takagi, Takuo Yasunaga, Hiroshi Jinnai, Kenji Iwasaki

JOURNAL OF ELECTRON MICROSCOPY　Vol. 61　No. 3　p. 193-198　2012/01　Research paper (scientific journal)

Functional Importance of Covalent Homodimer of Reelin Protein Linked via Its Central Region

Norihisa Yasui, Yu Kitago, Ayako Beppu, Takao Kohno, Shunsuke Morishita, Hiroki Gomi, Masamichi Nagae, Mitsuharu Hattori, Junichi Takagi

JOURNAL OF BIOLOGICAL CHEMISTRY　Vol. 286　No. 40　p. 35247-56　2011/10　Research paper (scientific journal)

Transient signalling complex formed by axon guidance cue semaphorin

Terukazu Nogi, Junichi Takagi

Seikagaku　Vol. 83　No. 10　p. 938-48　2011/10　Research paper (scientific journal)

Structural Basis for Variant-Specific Neuroligin-Binding by α-Neurexin

Tanaka, H, Nogi, T, Yasui, N, Iwasaki, K, Takagi, J

PLoS One　Vol. 6　No. 4　2011/04　Research paper (scientific journal)

Structural Basis for Variant-Specific Neuroligin-Binding by alpha-Neurexin

Hiroki Tanaka, Terukazu Nogi, Norihisa Yasui, Kenji Iwasaki, Junichi Takagi

PLOS ONE　Vol. 6　No. 4　2011/04　Research paper (scientific journal)

Crystal structure of autotaxin and insight into GPCR activation by lipid mediators

Hiroshi Nishimasu, Shinichi Okudaira, Kotaro Hama, Emiko Mihara, Naoshi Dohmae, Asuka Inoue, Ryuichiro Ishitani, Junichi Takagi, Junken Aoki, Osamu Nureki

NATURE STRUCTURAL & MOLECULAR BIOLOGY　Vol. 18　No. 2　p. 205-U271　2011/02　Research paper (scientific journal)

Crystal structure of autotaxin and insight into GPCR activation by lipid mediators.

Hiroshi Nishimasu, Shinichi Okudaira, Kotaro Hama, Emiko Mihara, Naoshi Dohmae, Asuka Inoue, Ryuichiro Ishitani, Junichi Takagi, Junken Aoki, Osamu Nureki

Nature structural & molecular biology　Vol. 18　No. 2　p. 205-12　2011/02　Research paper (scientific journal)

Crystallization and preliminary crystallographic analysis of human LR11 Vps10p domain

Zenzaburo Nakata, Masamichi Nagae, Norihisa Yasui, Hideaki Bujo, Terukazu Nogi, Junichi Takagi

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS　Vol. 67　No. Pt 1　p. 129-32　2011/01　Research paper (scientific journal)

Structural basis for semaphorin signalling through the plexin receptor.

Nogi T, Yasui N, Mihara E, Matsunaga Y, Noda M, Yamashita N, Toyofuku T, Uchiyama S, Goshima Y, Kumanogoh A, Takagi J

Nature　Vol. 467　No. 7319　p. 1123-U138　2010/10　Research paper (scientific journal)

Multiple discontinuous exosites for von willebrand factor of ADAMTS13

Masashi Akiyama, Soichi Takeda, Koichi Kokame, Junichi Takagi, Toshiyuki Miyata

Seikagaku　Vol. 82　No. 10　p. 950-6　2010/10　Research paper (scientific journal)

A rapid screening method for cell lines producing singly-tagged recombinant proteins using the "TARGET tag" system.

Tabata S, Nampo M, Mihara E, Tamura-Kawakami K, Fujii I, Takagi J

J Proteomics.　Vol. 73　No. 9　p. 1777-1785　2010/08　Research paper (scientific journal)

Comparison of the envelope architecture of E. coli using two methods: CEMOVIS and cryo-electron tomography.

Kishimoto-Okada A, Murakami S, Ito Y, Horii N, Furukawa H, Takagi J, Iwasaki K

J Electron Microsc (Tokyo).　Vol. 59　No. 5　p. 419-426　2010/07　Research paper (scientific journal)

Laminin-121--recombinant expression and interactions with integrins.

Sasaki T, Takagi J, Giudici C, Yamada Y, Arikawa-Hirasawa E, Deutzmann R, Timpl R, Sonnenberg A, Bächinger HP, Tonge D

Matrix Biol.　Vol. 29　No. 6　p. 484-493　2010/07　Research paper (scientific journal)

Structural basis for specific recognition of reelin by its receptors.

Yasui N, Nogi T, Takagi J

Structure　Vol. 18　No. 3　p. 320-331　2010/03　Research paper (scientific journal)

Detection of endogenous LRP6 expressed on human cells by monoclonal antibodies specific for the native conformation.

Yasui N, Mihara E, Nampo M, Tamura-Kawakami K, Unno H, Matsumoto K, Takagi J

J Immunol Methods.　Vol. 352　No. 1-2　p. 153-160　2010/01　Research paper (scientific journal)

Crystal structures of the noncatalytic domains of ADAMTS13 reveal multiple discontinuous exosites for von Willebrand factor.

Akiyama M, Takeda S, Kokame K, Takagi J, Miyata T

Proc Natl Acad Sci U S A.　Vol. 21　No. 3　p. 319-326　2009/11　Research paper (scientific journal)

Crystal structures of the noncatalytic domains of ADAMTS13 reveal multiple discontinuous exosites for von Willebrand factor

Masashi Akiyama, Soichi Takeda, Koichi Kokame, Junichi Takagi, Toshiyuki Miyata

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　Vol. 106　No. 46　p. 19274-9　2009/11　Research paper (scientific journal)

[Development of a novel affinity tag system for the next generation structural biology].

Takagi J

Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme　Vol. 54　No. 12 Suppl　p. 1468-75　2009/09　Research paper (scientific journal)

Production, crystallization and preliminary crystallographic analysis of an exosite-containing fragment of human von Willebrand factor-cleaving proteinase ADAMTS13.

Akiyama M, Takeda S, Kokame K, Takagi J, Miyata T

Acta Crystallogr Sect F Struct Biol Cryst Commun.　Vol. 65　No. Pt 7　p. 739-742　2009/07　Research paper (scientific journal)

Molecular basis of the recognition of nephronectin by integrin alpha8beta1.

Sato, Y, Uemura, T, Morimitsu, K, Sato-Nishiuchi, R, Manabe, R, Takagi, J, Yamada, M, Sekiguchi, K

J. Biol. Chem.　Vol. 284　No. 21　p. 14524-14536　2009/05　Research paper (scientific journal)

Novel affinity tag system using structurally defined antibody-tag interaction: application to single-step protein purification.

Nogi T, Sangawa T, Tabata S, Nagae M, Tamura-Kawakami K, Beppu A, Hattori M, Yasui N, Takagi J

Protein Sci.　Vol. 17　No. 12　p. 2120-2126　2008/12　Research paper (scientific journal)

Structure of the F-spondin reeler domain reveals a unique beta-sandwich fold with a deformable disulfide-bonded loop

Masamichi Nagae, Ken Nishikawa, Norihisa Yasui, Motoo Yamasaki, Terukazu Nogi, Junichi Takagi

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY　Vol. 64　p. 1138-1145　2008/11　Research paper (scientific journal)

Structure of F-spondin reeler domain reveals a unique b-sandwich fold with a deformable disulfide-bonded loop.

Nagae, M, Nishikawa, K, Yasui, N, Yamasaki, M, Nogi, T, Takagi, J

Acta Crystallographica., D　Vol. 64　No. Pt 11　p. 1138-45　2008/11　Research paper (scientific journal)

A murine monoclonal antibody that binds N-terminal extracellular segment of human protease activated receptor-4.

Sangawa, T, Nogi, T, Takagi, J

Hybridoma　Vol. 27　No. 5　p. 331-335　2008/07　Research paper (scientific journal)

Key interactions in ectodomain of integrin for global conformational change detected by elastic network normal mode analysis.

Atsushi Matsumoto, Tetsuji Kamata, Junichi Takagi, Kenji Iwasaki, Kei Yura

Biophys. J　Vol. 95　No. 6　p. 2895-2908　2008/06　Research paper (scientific journal)

3P-069 Development of Three-Dimensional Reconstruction System by integration of many processing tools(The 46th Annual Meeting of the Biophysical Society of Japan)

Nina Hitoshi, Taniguchi Kanae, Nishino Yuri, Iwasaki Kenji, Takagi Junichi, Miyazawa Atsuo, Mitsuoka Kaoru, Yasunaga Takuo

Seibutsu Butsuri　Vol. 48　2008

Crystal structure of Reelin repeats

Junichi Takagi

Reelin Glycoprotein: Structure, Biology and Roles in Health and Disease　p. 57-67　2008　Part of collection (book)

Milk fat globule EGF factor 8 in the serum of human patients of systemic lupus erythematosus.

Yamaguchi, H, Takagi, J, Miyamae, T, Yokota, S, Fujimoto, T, Nakamura, S, Ohshima, S, Naka, T, Nagata, S

J. Leukocyte Biol.　Vol. 83　No. 5　p. 1300-1307　2007/08　Research paper (scientific journal)

Three-dimensional architecture of virus-packed tubule

Katayama, S, Wei, T, Omura, T, Takagi, J, Iwasaki, K

Journal of Electron Microscopy　Vol. 56　No. 3　p. 77-81　2007/06　Research paper (scientific journal)

Structure of a receptor-binding fragment of reelin and mutational analysis reveal a recognition mechanism similar to endocytic receptors.

Yasui, N, Nogi, T, Kitao, T, Nakano, Y, Hattori, M, Takagi, J

Proc. Natl. Acad. Sci. USA　Vol. 104　No. 24　p. 9988-9993　2007/06　Research paper (scientific journal)

Semaphorin 7A initiates T cell-mediated inflammatory responses through alpha1beta1 integrin

Suzuki K, Okuno,T, Yamamoto, M, Pasterkamp, R.J, Takegahara, N, Kitao, T, Takagi, J, Rennert , P.D, Kolodkin, A.L, Kumanogoh, A, Kikutani, H

Nature　Vol. 446　No. 7136　p. 680-684　2007/05　Research paper (scientific journal)

3P288 Development of GUI-based tools for three dimensional electron tomography(Bioimaging,Poster Presentations)

Goto Tatsunori, Nagai Rina, Imamura Motoki, Matsuura Ayumi, Takagi Junichi, Iwasaki Kenji, Yasunaga Takuo

Seibutsu Butsuri　Vol. 47　No. 0　2007/04

alpha V beta 6 is a novel receptor for human fibrillin-1 - Comparative studies of molecular determinants underlying integrin-RGD affinityand specificity

Jelena Jovanovic, Junichi Takagi, Laurence Choulier, Nicola G. A. Abrescia, David I. Stuart, P. Anton van der Merwe, Helen J. Mardon, Penny A. Handford

JOURNAL OF BIOLOGICAL CHEMISTRY　Vol. 282　No. 9　p. 6743-6751　2007/03　Research paper (scientific journal)

The RGD motif in fibronectin is essential for mouse development but dispensable for fibronectin fibril assembly.

Takahashi, S, Leiss, M, Moser, M, Ohashi, T, Kitao, T, Heckmann, D, Pfeifer, A, Kessler, H, Takagi, J, Erickson, H.P, Fässler, R

J. Cell Biol.　Vol. 178　No. 1　p. 167-178　2007/03　Research paper (scientific journal)

1P043 Snap residues of integrin activation identified by elastic network normal mode analysis(Proteins-functions,Poster Presentations)

Matsumoto Atsushi, Kamata Tetsuji, Takagi Junichi, Iwasaki Kenji, Yura Kei

Seibutsu Butsuri　Vol. 47　2007

Structural Analysis of Modular Proteins : A Case of Integrin Head Fragment

TAKAGI Junichi

X-RAYS　Vol. 48　No. 6　p. 411-417　2006/12

モジュラータンパク質の構造解析：受容体構造生物学における中心課題

高木淳一

日本結晶学会誌　2006/10

Structure of a signaling-competent reelin fragment revealed by X-ray crystallography and electron tomography.

Nogi, T, Yasui, N, Hattori, M, Iwasaki, K, Takagi, J

EMBO Journal　Vol. 25　No. 15　p. 3675-3683　2006/07　Research paper (scientific journal)

Ligand-binding specificities of laminin-binding integrins: a comprehensive survey of laminin-integrin interactions using recombinant alpha3beta1, alpha6beta1, alpha7beta1 and alpha6beta4 integrins.

Nishiuchi, R, Takagi, J, Hayashi, M, Ido, H, Yagi, Y, Sanzen, N, Tsuji, T, Yamada, M, Sekiguchi, K

Matrix Biol.　Vol. 25　No. 3　p. 189-197　2006/06　Research paper (scientific journal)

Direct interaction of the kringle domain of urokinase-type plasminogen activator (uPA) and integrin alpha v beta 3 induces signal transduction and enhances plasminogen activation.

Tarui, T, Akakura, N, Majumdar, M, Andronicos, N, Takagi, J, Mazar, A.P, Bdeir, K, Kuo, A, Yarovoi, S.V, Cines, D.B, Takada, Y

Thromb Haemost.　Vol. 95　No. 3　p. 524-534　2006/04　Research paper (scientific journal)

[Dynamic regulation of receptor transmembrane domain assembly during signal transmission across the plasma membrane].

Takagi J

Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme　Vol. 50　No. 10 Suppl　p. 1226-32　2005/08

New era of structural analysis of proteins by electron microscopy - Application to a dynamic structural analysis of integrin cell adhesion receptor

K Iwasaki, J Takagi

SEIKAGAKU　Vol. 77　No. 5　p. 400-10　2005/05

Disrupting integrin transmembrane domain heterodimerization increases ligand binding affinity, not valency or clustering

BH Luo, CV Carman, J Takagi, TA Springer

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　Vol. 102　No. 10　p. 3679-3684　2005/03　Research paper (scientific journal)

Potentiation of the ligand-binding activity of integrin alpha3 beta1 via association with tetraspanin CD151.

Nishiuchi, R, Sanzen, N, Nada, S, Sumida, Y, Wada, Y, Okada, M, Takagi, J, Hasegawa, H, Sekiguchi, K

Proc. Natl. Acad. Sci. U S A　Vol. 102　No. 6　p. 1939-1944　2005/02　Research paper (scientific journal)

The relative influence of metal ion binding sites in the I-like domain and the interface with the hybrid domain on rolling and firm adhesion by integrin alpha(4)beta(7)

JF Chen, J Takagi, C Xie, T Xiao, BH Luo, TA Springer

JOURNAL OF BIOLOGICAL CHEMISTRY　Vol. 279　No. 53　p. 55556-55561　2004/12　Research paper (scientific journal)

Structural basis for allostery in integrins and binding to fibrinogen-mimetic therapeutics

T Xiao, J Takagi, BS Coller, JH Wang, TA Springer

NATURE　Vol. 432　No. 7013　p. 59-67　2004/11　Research paper (scientific journal)

Integrin beta 3 regions controlling binding of murine mAb 7E3: Implications for the mechanism of integrin alpha IIb beta 3 activation

A Artoni, JH Li, B Mitchell, J Ruan, J Takagi, TA Springer, DL French, BS Coller

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　Vol. 101　No. 36　p. 13114-20　2004/09　Research paper (scientific journal)

Integrin b3 regions controlling binding of murine mAb 7E3: Implications for the mechanism of integrin aIIbb3 activation.

Artoni A, Li J, Mitchell B, Ruan J, Takagi J, Springer TA, French DL, Coller BS

Proc. Natl. Acad. Sci. USA.　2004/07　Research paper (scientific journal)

A specific interface between integrin transmembrane helices and affinity for ligand.

Luo B-H, Springer TA, Takagi J

PLoS Biology　Vol. 2　No. 6　2004/06　Research paper (scientific journal)

A specific interface between integrin transmembrane helices and affinity for ligand

BH Luo, TA Springer, J Takagi

PLOS BIOLOGY　Vol. 2　No. 6　p. 776-786　2004/06　Research paper (scientific journal)

Allosteric b1 integrin antibodies that stabilize the low affinity state by preventing the swing-out of the hybrid domain.

Luo B-H, Strokovich K, Walz T, Springer TA, Takagi J

J. Biol. Chem.　Vol. 279　No. 26　p. 27466-27471　2004/04　Research paper (scientific journal)

Intersubunit signal transmission in integrins by a receptor-like interaction with a pull spring.

Yang W, Shimaoka M, Salas A, Takagi J, Springer TA

Proc. Natl. Acad. Sci. USA.　Vol. 101　No. 9　p. 2906-2911　2004/03　Research paper (scientific journal)

Locking the beta3 integrin I-like domain into high and low affinity conformations with disulfides.

Luo, B-H, Takagi, J, Springer, T. A

J. Biol. Chem.　Vol. 279　No. 11　p. 10215-10221　2004/03　Research paper (scientific journal)

3SB56 Structural rearrangement of integrins in transmembrane signaling

Takagi J.

Seibutsu Butsuri　Vol. 44　2004

[Complex between nidogen and laminin fragments reveals a paradigmatic beta-propeller interface].

Takagi J

Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme　Vol. 48　No. 14　p. 1920-7　2003/11

Structure of integrin a5b1 in complex with fibronectin.

Takagi, J, Konstantin, S, Springer, T. A, Walz, T

EMBO J.　Vol. 22　No. 18　p. 4607-4615　2003/09　Research paper (scientific journal)

Complex between nidogen and laminin fragments reveales a paradigmatic b-propeller interface.

Takagi, J, Yang, Y, Liu, J.-h, Wang, J.-h, Springer, T. A

Nature　2003/08　Research paper (scientific journal)

High affinity ligand binding by integrins does not involve head separation.

Luo B-H, Springer TA, Takagi J

J. Biol. Chem.　Vol. 278　No. 19　p. 17185-17189　2003/05　Research paper (scientific journal)

Stabilizing the open conformation of the integrin headpiece with a glycan wedge increases affinity for ligand.

Luo B-H, Springer TA, Takagi J

Proc. Natl. Acad. Sci. USA.　Vol. 100　No. 5　p. 2403-2408　2003/03　Research paper (scientific journal)

Duplication (22)(q11.22-q11.23) without coloboma and cleft lip or palate.

Sonoda T, Kouno K, Sawada K, Takagi J, Nunoi H, Harada N, Matsumoto N

Pediatrics international : official journal of the Japan Pediatric Society　Vol. 45　No. 1　p. 97-9　2003/02　Research paper (scientific journal)

Global conformational rearrangements in integrin extracellular domains in outside-in and inside-out signaling

JUNICHI TAKAGI

CELL　Vol. 110　No. 5　p. 599-611　2003/01　Research paper (scientific journal)

Structures of the alphaL I domain and its complex with ICAM-1 reveal a shape-shifting pathway for integrin regulation.

Shimaoka M, Xiao T, Liu J-H, Yang Y, Dong Y, Jun C-D, McCormack A, Zhang R, Joachimiak A, Takagi J, Wang J-H, Springer TA

Cell　Vol. 112　No. 1　p. 99-111　2003/01　Research paper (scientific journal)

A complex between nidogen and laminin fragments reveales a paradigmatic b-propeller bound to laminin reveals a mode of binding shared with developmental pathway and endocytic receptors.

Nature　Vol. 424, 969-974/,　2003

Stabilizing the integrin alpha M inserted domain in alternative conformations with a range of engineered disulfide bonds

M Shimaoka, CF Lu, A Salas, T Xiao, J Takagi, TA Springer

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　Vol. 99　No. 26　p. 16737-41　2002/12　Research paper (scientific journal)

Archaeal surface layer proteins contain beta propeller, PKD, and beta helix domains and are related to metazoan cell surface proteins

H Jing, J Takagi, JH Liu, S Lindgren, RG Zhang, A Joachimiak, JH Wang, TA Springer

STRUCTURE　Vol. 10　No. 10　p. 1453-64　2002/10　Research paper (scientific journal)

Global conformational rearrangements in integrin extracellular domains in outside-in and inside-out signaling.

Takagi J, Petre BM, Walz T, Springer TA

Cell　Vol. 14　No. 1　p. 3-10　2002/09　Research paper (scientific journal)

Co-occurrence of Down syndrome and Treacher-Collins syndrome.

Sonoda T, Sawada K, Kouno K, Takagi J, Ikeda T, Sameshima H, Ikenoue T

Pediatrics international : official journal of the Japan Pediatric Society　Vol. 44　No. 4　p. 440-2　2002/08　Research paper (scientific journal)

Integrin activation and structural rearrangement

J Takagi, TA Springer

IMMUNOLOGICAL REVIEWS　Vol. 186　p. 141-163　2002/08

Cysteine-rich module structure reveals a fulcrum for integrin rearrangement upon activation

N Beglova, SC Blacklow, J Takagi, TA Springer

NATURE STRUCTURAL BIOLOGY　Vol. 9　No. 4　p. 282-7　2002/04　Research paper (scientific journal)

Role of the specificity-determining loop of the integrin beta subunit I-like domain in autonomous expression, association with the alpha subunit, and ligand binding

J Takagi, DP DeBottis, HP Erickson, TA Springer

BIOCHEMISTRY　Vol. 41　No. 13　p. 4339-4347　2002/04　Research paper (scientific journal)

Role of the specificity-determining loop of the integrin beta subunit I-like domain in autonomous expression, association with the alpha subunit, and ligand binding

J Takagi, DP DeBottis, HP Erickson, TA Springer

BIOCHEMISTRY　Vol. 41　No. 13　p. 4339-47　2002/04　Research paper (scientific journal)

Conformational regulation of integrin structure and function

M Shimaoka, J Takagi, TA Springer

ANNUAL REVIEW OF BIOPHYSICS AND BIOMOLECULAR STRUCTURE　Vol. 31　p. 485-516　2002/04　Research paper (scientific journal)

Fibronectin peptides derived from two distinct regions stimulate adipocyte differentiation by preventing fibronectin matrix assembly

SL Kamiya, R Kato, M Wakabayashi, T Tohyama, Enami, I, M Ueki, HL Yajima, T Ishii, H Nakamura, T Katayama, J Takagi, F Fukai

BIOCHEMISTRY　Vol. 41　No. 9　p. 3270-7　2002/03　Research paper (scientific journal)

The thioesterase domain from a nonribosomal peptide synthetase as a cyclization catalyst for integrin binding peptides

RM Kohli, J Takagi, CT Walsh

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　Vol. 99　No. 3　p. 1247-52　2002/02　Research paper (scientific journal)

[First atomic view of alpha V beta 3 integrin extracellular domain].

Takagi J

Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme　Vol. 47　No. 2　p. 153-9　2002/02

Definition of EGF-like, closely interacting modules that bear activation epitopes in integrin beta subunits

J Takagi, N Beglova, P Yalamanchili, SC Blacklow, TA Springer

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　Vol. 98　No. 20　p. 11175-11180　2001/09　Research paper (scientific journal)

Implications for familial hypercholesterolemia from the structure of the LDL receptor YWTD-EGF domain pair

H Jeon, WY Meng, J Takagi, MJ Eck, TA Springer, SC Blacklow

NATURE STRUCTURAL BIOLOGY　Vol. 8　No. 6　p. 499-504　2001/06　Research paper (scientific journal)

Dimerization and the effectiveness of ICAM-1 in mediating LFA-l-dependent adhesion

CD Jun, M Shimaoka, CV Carman, J Takagi, TA Springer

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　Vol. 98　No. 12　p. 6830-6835　2001/06　Research paper (scientific journal)

C-terminal opening mimics 'inside-out' activation of integrin alpha 5 beta 1

J Takagi, HP Erickson, TA Springer

NATURE STRUCTURAL BIOLOGY　Vol. 8　No. 5　p. 412-416　2001/05　Research paper (scientific journal)

Epitope mapping of antibodies to the C-terminal region of the integrin beta(2) subunit reveals regions that become exposed upon receptor activation

CF Lu, M Ferzly, J Takagi, TA Springer

JOURNAL OF IMMUNOLOGY　Vol. 166　No. 9　p. 5629-5637　2001/05　Research paper (scientific journal)

Association of the membrane proximal regions of the alpha and beta subunit cytoplasmic domains constrains an integrin in the inactive state

CF Lu, J Takagi, TA Springer

JOURNAL OF BIOLOGICAL CHEMISTRY　Vol. 276　No. 18　p. 14642-14648　2001/05　Research paper (scientific journal)

Reversibly locking a protein fold in an active conformation with a disulfide bond: Integrin alpha L I domains with high affinity and antagonist activity in vivo

M Shimaoka, CF Lu, RT Palframan, UH von Andrian, A McCormack, J Takagi, TA Springer

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　Vol. 98　No. 11　p. 6009-6014　2001/05　Research paper (scientific journal)

Locking in alternate conformations of the integrin alpha L beta 2 I domain with disulfide bonds reveals functional relationships among integrin domains

CF Lu, M Shimaoka, Q Zang, J Takagi, TA Springer

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　Vol. 98　No. 5　p. 2393-2398　2001/02　Research paper (scientific journal)

An isolated, surface-expressed I domain of the integrin alpha L beta 2 is sufficient for strong adhesive function when locked in the open conformation with a disulfide bond

CF Lu, M Shimaoka, N Ferzly, C Oxvig, J Takagi, TA Springer

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　Vol. 98　No. 5　p. 2387-2392　2001/02　Research paper (scientific journal)

C-terminal opening can mimic "inside-out" activation of integrin alpha 5 beta 1.

J Takagi, HP Erickson, TA Springer

BIOPHYSICAL JOURNAL　Vol. 80　No. 1　p. 333A-334A　2001/01

Tissue transglutaminase, coagulation factor XIII, and the pro-polypeptide of von Willebrand factor are all ligands for the integrins α9β1 and α4β1

Hiroo Takahashi, Takashi Isobe, Shiho Horibe, Junichi Takagi, Yasuyuki Yokosaki, Dean Sheppardi, Yuji Saito

Journal of Biological Chemistry　Vol. 275　No. 31　p. 23589-23595　2000/08/04　Research paper (scientific journal)

Computational design of an integrin I domain stabilized in the open high affinity conformation.

M Shimaoka, J M Shifman, H Jing, J Takagi, S L Mayo, T A Springer

Nature structural biology　Vol. 7　No. 8　p. 674-8　2000/08　Research paper (scientific journal)

The top of the inserted-like domain of the integrin lymphocyte function-associated antigen-1 beta subunit contacts the alpha subunit beta-propeller domain near beta-sheet 3

Q Zang, CF Lu, CC Huang, J Takagi, TA Springer

JOURNAL OF BIOLOGICAL CHEMISTRY　Vol. 275　No. 29　p. 22202-22212　2000/07　Research paper (scientific journal)

Structural and functional studies with antibodies to the integrin beta 2 subunit - A model for the I-like domain

CC Huang, Q Zang, J Takagi, TA Springer

JOURNAL OF BIOLOGICAL CHEMISTRY　Vol. 275　No. 28　p. 21514-21524　2000/07　Research paper (scientific journal)

12-hydroxy-5Z, 8Z, 10E, 14Z, eicosatetraenoic acid (12-HETE) stimulates cAMP production in normal human fibroblasts

Shoko Ueki, Junichi Takagi, Yuichi Kobayashi, Fumie Sato, Yuji Saito

Journal of Cellular Physiology　Vol. 178　No. 1　p. 63-68　1999/01　Research paper (scientific journal)

Activity-independent cell adhesion to tissue-type transglutaminase is mediated by α4β1 integrin

Takashi Isobe, Hiroo Takahashi, Shoko Ueki, Junichi Takagi, Yuji Saito

European Journal of Cell Biology　Vol. 78　No. 12　p. 876-883　1999　Research paper (scientific journal)

Expression of the molecule detectable by anti-propolypeptide of Von Willebrand factor antibody in rat mesangial cells in anti-Thy 1.1 mAb 1-22-3 induced glomerulonephritis: A marker of injured mesangial cells

Minako Wakasugi, Hiroshi Kawachi, Saori Omori, Junichi Takagi, Shinichi Nishi, Masaaki Arakawa, Fujio Shimizu

Nephron　Vol. 82　No. 4　p. 338-347　1999　Research paper (scientific journal)

Two-stage activation for α5β1 integrin binding to surface-adsorbed fibronectin

Andrés J. García, Junichi Takagi, David Boettiger

Journal of Biological Chemistry　Vol. 273　No. 52　p. 34710-34715　1998/12/25　Research paper (scientific journal)

The 'ligand-induced conformational change' of alpha 5 beta 1 integrin - Relocation of alpha 5 subunit to uncover the beta 1 stalk region

J Tsuchida, S Ueki, Y Takada, Y Saito, J Takagi

JOURNAL OF CELL SCIENCE　Vol. 111　p. 1759-1766　1998/06　Research paper (scientific journal)

Screening of hybridoma clones producing antibodies against plasma membrane-associated materials

A. I. Kushida, Masaki Koga, Junichi Takagi, Yuji Saito

Hybridoma　Vol. 17　No. 2　p. 209-213　1998　Research paper (scientific journal)

Factor X-dependent, thrombin-generating activities on a neuroblastoma cell and their disappearance upon differentiation

Hiroki Inuyama, Takashi Saito, Junichi Takagi, Yuji Saito

Journal of Cellular Physiology　Vol. 173　No. 3　p. 406-414　1997/12　Research paper (scientific journal)

Classification of 'activation' antibodies against integrin β1 chain

Jun Tsuchida, Shoko Ueki, Yuji Saito, Junichi Takagi

FEBS Letters　Vol. 416　No. 2　p. 212-216　1997/10/20　Research paper (scientific journal)

Changing ligand specificities of alpha v beta 1 and alpha v beta 3 integrins by swapping a short diverse sequence of the beta subunit

J Takagi, T Kamata, J Meredith, W PuzonMcLaughlin, Y Takada

JOURNAL OF BIOLOGICAL CHEMISTRY　Vol. 272　No. 32　p. 19794-19800　1997/08　Research paper (scientific journal)

Propolypeptide of von Willebrand factor is a novel ligand for very late antigen-4 integrin

Takashi Isobe, Tetsuya Hisaoka, Akira Shimizu, Mitsuhiro Okuno, Saburo Aimoto, Yoshikazu Takada, Yuji Saito, Junichi Takagi

Journal of Biological Chemistry　Vol. 272　No. 13　p. 8447-8453　1997/03/28　Research paper (scientific journal)

Structural interlock between ligand-binding site and stalk-like region of β1 integrin revealed by a monoclonal antibody recognizing conformation-dependent epitope

Junichi Takagi, Takashi Isobe, Yoshikazu Takada, Yuji Saito

Journal of Biochemistry　Vol. 121　No. 5　p. 914-921　1997　Research paper (scientific journal)

Inhibition of adenylyl cyclases by 12(S)-hydroxyeicosatetraenoic acid

Xiao-Quan Wang, Masaaki Otsuka, Junichi Takagi, Yuichi Kobayashi, Fumie Sato, Yuji Saito

Biochemical and Biophysical Research Communications　Vol. 228　No. 1　p. 81-87　1996/11/01　Research paper (scientific journal)

Dual functions of transglutaminase in novel cell adhesion

Shoko Ueki, Junichi Takagi, Yuji Saito

Journal of Cell Science　Vol. 109　No. 11　p. 2727-2735　1996/11　Research paper (scientific journal)

A fruiting body-specific cDNA, mfbAc, from the mushroom Lentinus edodes encodes a high-molecular-weight cell-adhesion protein containing an Arg-Gly-Asp motif

Osamu Kondoh, Akihiko Muto, Susumu Kajiwara, Junichi Takagi, Yuji Saito, Kazuo Shishido

Gene　Vol. 154　No. 1　p. 31-37　1995/02/27　Research paper (scientific journal)

Identification of Factor‐XIIIa‐Reactive Glutaminyl Residues in the Propolypeptide of Bovine von Willebrand Factor

Junichi Takagi, Toshihiro Aoyama, Shoko Ueki, Hirotsugu Ohba, Yuji Saito, Laszlo Lorand

European Journal of Biochemistry　Vol. 232　No. 3　p. 773-777　1995　Research paper (scientific journal)

Differential Role of Protein Tyrosine Phosphorylation/dephosphorylation in Affinity Regulation of β1 and β3 Integrin in Human Fibroblasts

Junichi Takagi, Yuji Saito

Cell Structure and Function　Vol. 20　No. 5　p. 403-413　1995　Research paper (scientific journal)

β1‐integrin‐mediated adhesion of melanoma cells to the propolypeptide of von Willebrand factor

Junichi TAKAGI, Yoshimitsu SUDO, Takashi SAITO, Yuji SAITO

European Journal of Biochemistry　Vol. 222　No. 3　p. 861-868　1994　Research paper (scientific journal)

Propolypeptide of von Willebrand factor serves as a substrate for factor XIIIa and is cross-linked to laminin

Tomoko Usui, Junichi Takagi, Yuji Saito

Journal of Biological Chemistry　Vol. 268　No. 17　p. 12311-12316　1993/06/15　Research paper (scientific journal)

A single chain 19-kDa fragment from bovine thrombospondin binds to type V collagen and heparin

J. Takagi, T. Fujisawa, T. Usui, T. Aoyama, Y. Saito

Journal of Biological Chemistry　Vol. 268　No. 21　p. 15544-15549　1993　Research paper (scientific journal)

Activation of phospholipases in platelets by polyclonal antibodies against a surface membrane protein

Keiko Hashimoto, Fujio Sekiya, Junichi Takagi, Toshiyasu Tsukada, Fumie Sato, Yuji Saito

Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism　Vol. 1165　No. 1　p. 27-31　1992/11/11　Research paper (scientific journal)

A Collagen/Gelatin-Binding Decapeptide Derived from Bovine Propolypeptide of von Willebrand Factor

Junichi Takagi, Hiroto Asai, Yuji Saito

Biochemistry　Vol. 31　No. 36　p. 8530-8534　1992/02/01　Research paper (scientific journal)

Propolypeptide and mature portions of von Willebrand factor of bovine origin recognize different sites on type‐I collagen obtained from bovine tendon

Tomoko USUI, Tomoyuki FUJISAWA, Junichi TAKAGI, Yuji SAITO

European Journal of Biochemistry　Vol. 205　No. 1　p. 363-367　1992　Research paper (scientific journal)

12S-Hydroxyeicosatetraenoic acid plays a central role in the regulation of platelet activation

Fujio Sekiya, Junichi Takagi, Tomoko Usui, Keiko Kawajiri, Yuichi Kobayashi, Fumie Sato, Yuji Saito

Biochemical and Biophysical Research Communications　Vol. 179　No. 1　p. 345-351　1991/08/30　Research paper (scientific journal)

Immunological detection of propolypeptide of von Willebrand factor on platelet surface

Keiko Hashimoto, Tomoko Usui, Ken-ichi Sasaki, Tomoyuki Fujisawa, Fujio Sekiya, Junichi Takagi, Toshiyasu Tsukada, Yuji Saito

Biochemical and Biophysical Research Communications　Vol. 176　No. 3　p. 1571-1576　1991/05/15　Research paper (scientific journal)

Collagen-binding domain within bovine propolypeptide of von willebrand factor

Junichi Takagi, Tomoyuki Fujisawa, Fujio Sekiya, Yuji Saito

Journal of Biological Chemistry　Vol. 266　No. 9　p. 5575-5579　1991　Research paper (scientific journal)

Monoclonal antibodies that inhibit binding of propolypeptide of von Willebrand factor to collagen: Localization of epitopes

Tomoyuki FUJISAWA, Junichi TAKAGI, Fujio SEKIYA, Akira GOTO, Fumio MIAKE, Yuji SAITO

European Journal of Biochemistry　Vol. 196　No. 3　p. 673-677　1991　Research paper (scientific journal)

Albumin in plasma potentiates platelet aggregation induced by collagen - a study with an albumin deficient rat -

Fujio Sekiya, Keiko Kawajiri, Junichi Takagi, Yuji Saito, Sumi Nagase

Thrombosis Research　Vol. 58　No. 6　p. 657-662　1990/06/15　Research paper (scientific journal)

Feedback regulation of platelet function by 12 S-hydroxyeicosatetraenoic acid: Inhibition of arachidonic acid liberation from phospholipids

Fujio Sekiya, Junichi Takagi, Ken-ichi Sasaki, Keiko Kawajiri, Yuichi Kobayashi, Fumie Sato, Yuji Saito

Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism　Vol. 1044　No. 1　p. 165-168　1990/05/01　Research paper (scientific journal)

Elucidation of a role of plasma albumin during collagen-induced aggregation of bovine platelets

Fujio Sekiya, Junichi Takagi, Yuji Saito

Thrombosis Research　Vol. 56　No. 3　p. 407-415　1989/11/01　Research paper (scientific journal)

Inhibition of platelet-collagen interaction by propolypeptide of von Willebrand factor

J. Takagi, F. Sekiya, K. Kasahara, Y. Inada, Y. Saito

Journal of Biological Chemistry　Vol. 264　No. 11　p. 6017-6020　1989　Research paper (scientific journal)

A collagen-binding glycoprotein from bovine platelets is identical to propolypeptide of von Willebrand factor

J. Takagi, K. Kasahara, F. Sekiya, Y. Inada, Y. Saito

Journal of Biological Chemistry　Vol. 264　No. 18　p. 10425-10430　1989　Research paper (scientific journal)

Subunit b of factor XIII is present in bovine platelets

Junichi Takagi, Kohji Kasahara, Fujio Sekiya, Yuji Inada, Yuji Saito

Thrombosis Research　Vol. 50　No. 6　p. 767-774　1988/06/15　Research paper (scientific journal)

Plasma albumin is essential for collagen-induced platelet aggregation

Fujio Sekiya, Junichi Takagi, Kohji Kasahara, Yuji Inada, Yuji Saito

Thrombosis Research　Vol. 50　No. 6　p. 837-846　1988/06/15　Research paper (scientific journal)

"A" subunit of factor XIII is present on bovine platelet membrane and mediates collagen-induced platelet activation

Kohji Kasahara, Junichi Takagi, Fujio Sekiya, Yuji Inada, Yuji Saito

Thrombosis Research　Vol. 50　No. 2　p. 253-263　1988/04/15　Research paper (scientific journal)

Venom from southern copperhead snake (Agkistrodon contortrix contortrix). II. A unique phospholipase A2 that induces platelet aggregation

Junichi Takagi, Fujio Sekiya, Kohji Kasahara, Yuji Inada, Yuji Saito

Toxicon　Vol. 26　No. 2　p. 199-206　1988　Research paper (scientific journal)

Analysis of distribution of receptors among platelets by flow cytometry

Kohji Kasahara, Junichi Takagi, Fujio Sekiya, Yuji Inada, Yuji Saito

Thrombosis Research　Vol. 45　No. 6　p. 763-770　1987/03/15　Research paper (scientific journal)

Venom from southern copperhead snake (Agkistrodon Contortrix Contortrix). I. Characterization of a protease that preferentially releases fibrinopeptide B

Akira Shimizu, Elsie C. Jimenez, Junichi Takagi, Yuji Inada, Yuji Saito

Toxicon　Vol. 25　No. 7　p. 751-757　1987　Research paper (scientific journal)

Interaction between plasma factor XIII and collagen

Takashi Kikuchi, Junichi Takagi, Kohji Kasahara, Yuji Inada, Yuji Saito

Thrombosis Research　Vol. 43　No. 2　p. 213-218　1986/07/15　Research paper (scientific journal)

Specific desensitization in the cytoskeletal assembly of platelet by receptor-dependent activations

T. Imada, Y. Saito, H. Shimada, T. Kikuchi, J. Takagi, Y. Inada

Thrombosis Research　Vol. 41　No. 1　p. 119-125　1986/01/01　Research paper (scientific journal)

Platelet Factor XIII. The collagen receptor?

Y. Saito, T. Imada, J. Takagi, T. Kikuchi, Y. Inada

Journal of Biological Chemistry　Vol. 261　No. 3　p. 1355-1358　1986　Research paper (scientific journal)

Modification of transglutaminase assay: Use of ammonium sulfate to stop the reaction

Junichi Takagi, Yuji Saito, Takashi Kikuchi, Yuji Inada

Analytical Biochemistry　Vol. 153　No. 2　p. 295-298　1986　Research paper (scientific journal)

A New Platelet Aggregation Factor from Gynostemma pentaphyllum Makino

Junichi Takagi, Teruaki Imada, Takashi Kikuchi, Yuji Saito, Yuji Inada

Chemical and Pharmaceutical Bulletin　Vol. 33　No. 12　p. 5568-5571　1985　Research paper (scientific journal)