Genetic engineering strategy for generating a stable dsRNA virus vector using a virus-like codon-modified transgene.

Yuta Kanai, Misa Onishi, Yukino Yoshida, Tomohiro Kotaki, Shohei Minami, Ryotaro Nouda, Moeko Yamasaki, Yasutaka Enoki, Takeshi Kobayashi

Journal of virology　Vol. 97　No. 10　2023/10/31　Research paper (scientific journal)

Electrolyzed hypochlorous acid water exhibits potent disinfectant activity against various viruses through irreversible protein aggregation.

Rahmi Dianty, Junki Hirano, Itsuki Anzai, Yuta Kanai, Tsuyoshi Hayashi, Masae Morimoto, Chikako Kataoka-Nakamura, Sakura Kobayashi, Kentaro Uemura, Chikako Ono, Tokiko Watanabe, Takeshi Kobayashi, Kosuke Murakami, Kenji Kikuchi, Kunimoto Hotta, Toshikazu Yoshikawa, Shuhei Taguwa, Yoshiharu Matsuura

Frontiers in microbiology　Vol. 14　p. 1284274-1284274　2023/10/01　Research paper (scientific journal)

Designing one-step reverse transcriptase loop-mediated isothermal amplification for serotype O foot-and-mouth disease virus detection during the 2022 outbreak in East Java, Indonesia.

Eduardus Bimo Aksono, Mirni Lamid, Rimayanti Rimayanti, Iwan Sahrial Hamid, Mustofa Helmi Effendi, Fedik Abdul Rantam, Widjiati Widjiati, Mufasirin Mufasirin, Heni Puspitasari, Munawaroh Fitria, Nur Syamsiatul Fajar, Lucia Tri Suwanti, Nusdianto Nusdianto, Andi Hamim Zaidan, Yuta Kanai, Teguh Hari Sucipto

Veterinary world　Vol. 16　No. 9　p. 1889-1896　2023/09/01　Research paper (scientific journal)

Effective SARS-CoV-2 replication of monolayers of intestinal epithelial cells differentiated from human induced pluripotent stem cells.

Shohei Minami, Naomi Matsumoto, Hiroko Omori, Yutaka Nakamura, Shigeyuki Tamiya, Ryotaro Nouda, Jeffery A Nurdin, Moeko Yamasaki, Tomohiro Kotaki, Yuta Kanai, Toru Okamoto, Taro Tachibana, Hiroshi Ushijima, Takeshi Kobayashi, Shintaro Sato

Scientific reports　Vol. 13　No. 1　p. 11610-11610　2023/07/18　Research paper (scientific journal)

RNAウイルスベクターの開発

小瀧将裕, 金井祐太, 小林 剛

医学のあゆみ　Vol. 285　2023/04/01　Research paper (scientific journal)

Characterization of Sialic Acid-Independent Simian Rotavirus Mutants in Viral Infection and Pathogenesis.

Moeko Yamasaki, Yuta Kanai, Yoshiki Wakamura, Tomohiro Kotaki, Shohei Minami, Ryotaro Nouda, Jeffery A Nurdin, Takeshi Kobayashi

Journal of virology　Vol. 97　No. 1　2023/01/05　Research paper (scientific journal)

N-Glycosylation of Rotavirus NSP4 Protein Affects Viral Replication and Pathogenesis.

Jeffery A Nurdin, Tomohiro Kotaki, Takahiro Kawagishi, Shintaro Sato, Moeko Yamasaki, Ryotaro Nouda, Shohei Minami, Yuta Kanai, Takeshi Kobayashi

Journal of virology　Vol. 97　No. 1　2023/01/04　Research paper (scientific journal)

A reverse genetics system for human rotavirus G2P[4]

Rina Hamajima, Tina Lusiany, Shohei Minami, Ryotaro Nouda, Jeffery A. Nurdin, Moeko Yamasaki, Nobumichi Kobayashi, Yuta Kanai, Takeshi Kobayashi

Journal of General Virology　Vol. 103　No. 12　2022/12/15　Research paper (scientific journal)

The nonstructural p17 protein of a fusogenic bat-borne reovirus regulates viral replication in virus species- and host-specific manners.

Ryotaro Nouda, Takahiro Kawagishi, Yuta Kanai, Masayuki Shimojima, Masayuki Saijo, Yoshiharu Matsuura, Takeshi Kobayashi

PLoS pathogens　Vol. 18　No. 6　p. e1010553-e1010553　2022/06/02　Research paper (scientific journal)

Ultrasensitive detection of SARS-CoV-2 nucleocapsid protein using large gold nanoparticle-enhanced surface plasmon resonance.

Taka-Aki Yano, Taira Kajisa, Masayuki Ono, Yoshiya Miyasaka, Yuichi Hasegawa, Atsushi Saito, Kunihiro Otsuka, Ayuko Sakane, Takuya Sasaki, Koji Yasutomo, Rina Hamajima, Yuta Kanai, Takeshi Kobayashi, Yoshiharu Matsuura, Makoto Itonaga, Takeshi Yasui

Scientific reports　Vol. 12　No. 1　p. 1060-1060　2022/01/20　Research paper (scientific journal)

Development of an entirely plasmid-based reverse genetics system for 12-segmented double-stranded RNA viruses.

Ryotaro Nouda, Shohei Minami, Yuta Kanai, Takahiro Kawagishi, Jeffery A Nurdin, Moeko Yamasaki, Ryusei Kuwata, Hiroshi Shimoda, Ken Maeda, Takeshi Kobayashi

Proceedings of the National Academy of Sciences of the United States of America　Vol. 118　No. 42　2021/10/19　Research paper (scientific journal)

Monoreassortant rotaviruses of multiple G types are differentially neutralized by sera from infants vaccinated with ROTARIX® and RotaTeq®.

Julia R Diller, Maximilian H Carter, Yuta Kanai, Shania V Sanchez, Takeshi Kobayashi, Kristen M Ogden

The Journal of infectious diseases　Vol. 224　No. 10　p. 1720-1729　2021/10/10　Research paper (scientific journal)

The Detection of Rotavirus Antigenemia by Immunochromatographic Kits: a Case Series.

Hiroshi Ushijima, Toshiyuki Hikita, Masaaki Kobayashi, Ngan Thi Kim Pham, Yuko Onda-Shimizu, Takahiro Kawagishi, Shoko Okitsu, Yuta Kanai, Takeshi Kobayashi, Tung Phan, Sheikh Ariful Hoque, Sayaka Takanashi, Satoshi Komoto, Kattareeya Kumthip, Koki Taniguchi, Niwat Maneekarn, Satoshi Hayakawa, Pattara Khamrin

Clinical laboratory　Vol. 67　No. 10　p. 2275-2282　2021/10/01　Research paper (scientific journal)

FAST Proteins: Development and Use of Reverse Genetics Systems for Reoviridae Viruses

Yuta Kanai, Takeshi Kobayashi

Annual Review of Virology　Vol. 8　No. 1　p. 515-536　2021/09/29

DsRNA sequencing for RNA virus surveillance using human clinical samples

Takuma Izumi, Yuhei Morioka, Syun Ichi Urayama, Daisuke Motooka, Tomokazu Tamura, Takahiro Kawagishi, Yuta Kanai, Takeshi Kobayashi, Chikako Ono, Akinari Morinaga, Takahiro Tomiyama, Norifumi Iseda, Yukiko Kosai, Shoichi Inokuchi, Shota Nakamura, Tomohisa Tanaka, Kohji Moriishi, Hiroaki Kariwa, Tomoharu Yoshizumi, Masaki Mori, Yoshiharu Matsuura, Takasuke Fukuhara

Viruses　Vol. 13　No. 7　2021/07/01　Research paper (scientific journal)

Combining machine learning and nanopore construction creates an artificial intelligence nanopore for coronavirus detection.

Masateru Taniguchi, Shohei Minami, Chikako Ono, Rina Hamajima, Ayumi Morimura, Shigeto Hamaguchi, Yukihiro Akeda, Yuta Kanai, Takeshi Kobayashi, Wataru Kamitani, Yutaka Terada, Koichiro Suzuki, Nobuaki Hatori, Yoshiaki Yamagishi, Nobuei Washizu, Hiroyasu Takei, Osamu Sakamoto, Norihiko Naono, Kenji Tatematsu, Takashi Washio, Yoshiharu Matsuura, Kazunori Tomono

Nature communications　Vol. 12　No. 1　p. 3726-3726　2021/06/17　Research paper (scientific journal)

Epidemiology and genetic diversity of group A rotaviruses in pre-vaccination period in Thailand, 2018-2019.

Nutthawadee Jampanil, Kattareeya Kumthip, Arpaporn Yodmeeklin, Yuta Kanai, Shoko Okitsu, Takeshi Kobayashi, Nuthapong Ukarapol, Hiroshi Ushijima, Niwat Maneekarn, Pattara Khamrin

Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases　Vol. 95　p. 104898-104898　2021/05/07　Research paper (scientific journal)

Rotavirus reverse genetics systems: Development and application

Yuta Kanai, Takeshi Kobayashi

Virus Research　Vol. 295　p. 198296-198296　2021/04/02

Generation of recombinant rotaviruses encoding a split NanoLuc peptide tag

Pimfhun Pannacha, Yuta Kanai, Takahiro Kawagishi, Ryotaro Nouda, Jeffery A. Nurdin, Moeko Yamasaki, Keiichiro Nomura, Tina Lusiany, Takeshi Kobayashi

Biochemical and Biophysical Research Communications　Vol. 534　p. 740-746　2021/01/01　Research paper (scientific journal)

Reverse genetics approach for developing rotavirus vaccine candidates carrying VP4 and VP7 genes cloned from clinical isolates of human rotavirus

Yuta Kanai, Misa Onishi, Takahiro Kawagishi, Pimfhun Pannacha, Jeffery A. Nurdin, Ryotaro Nouda, Moeko Yamasaki, Tina Lusiany, Pattara Khamrin, Shoko Okitsu, Satoshi Hayakawa, Hirotaka Ebina, Hiroshi Ushijima, Takeshi Kobayashi

Journal of Virology　Vol. 95　No. 2　2021/01　Research paper (scientific journal)

Generation of Genetically RGD σ1-Modified Oncolytic Reovirus That Enhances JAM-A-Independent Infection of Tumor Cells

Takahiro Kawagishi, Yuta Kanai, Ryotaro Nouda, Ichika Fukui, Jeffery A. Nurdin, Yoshiharu Matsuura, Takeshi Kobayashi

Journal of Virology　Vol. 94　No. 23　2020/12　Research paper (scientific journal)

An artificial intelligence nanopore platform for SARS-CoV-2 virus detection

Masateru Taniguchi, Shohei Minami, Chikako Ono, Rina Hamajima, Ayumi Morimura, Shigeto Hamaguchi, Yukihiro Akeda, Yuta Kanai, Takeshi Kobayashi, Wataru Kamitani, Yutaka Terada, Koichiro Suzuki, Nobuaki Hatori, Yoshiaki Yamagishi, Nobuei Washizu, Hiroyasu Takei, Osamu Sakamoto, Norihiko Naono, Kenji Tatematsu, Takashi Washio, Yoshiharu Matsuura, Kazunori Tomono

2020/10/28

Reverse genetics system for a human group A rotavirus

Takahiro Kawagishi, Jeffery A. Nurdin, Misa Onishi, Ryotaro Nouda, Yuta Kanai, Takeshi Tajima, Hiroshi Ushijima, Takeshi Kobayashi

Journal of Virology　Vol. 94　No. 2　2020/01/01　Research paper (scientific journal)

In vivo live imaging of oncolytic mammalian orthoreovirus expressing nanoluc luciferase in tumor xenograft mice

Yuta Kanai, Takahiro Kawagishi, Yoshiharu Matsuura, Takeshi Kobayashi

Journal of Virology　Vol. 93　No. 14　2019/07　Research paper (scientific journal)

Cell-cell fusion induced by reovirus FAST proteins enhances replication and pathogenicity of non-enveloped dsRNA viruses

Yuta Kanai, Takahiro Kawagishi, Yusuke Sakai, Ryotaro Nouda, Masayuki Shimojima, Masayuki Saijo, Yoshiharu Matsuura, Takeshi Kobayashi

PLoS Pathogens　Vol. 15　No. 4　2019/04　Research paper (scientific journal)

Development of stable rotavirus reporter expression systems

Yuta Kanai, Takahiro Kawagishi, Ryotaro Nouda, Misa Onishi, Pimfhun Pannacha, Jeffery A. Nurdin, Keiichiro Nomura, Yoshiharu Matsuura, Takeshi Kobayashi

Journal of Virology　Vol. 93　No. 4　2019/02/01　Research paper (scientific journal)

Lethal murine infection model for human respiratory disease-associated Pteropine orthoreovirus

Yuta Kanai, Takahiro Kawagishi, Minoru Okamoto, Yusuke Sakai, Yoshiharu Matsuura, Takeshi Kobayashi

Virology　Vol. 514　p. 57-65　2018/01/15　Research paper (scientific journal)

Reverse genetics system demonstrates that rotavirus nonstructural protein NSP6 is not essential for viral replication in cell cultures

Satoshi Komoto, Yuta Kanai, Saori Fukuda, Masanori Kugita, Takahiro Kawagishi, Naoto Ito, Makoto Sugiyama, Yoshiharu Matsuura, Takeshi Kobayashi, Koki Taniguchi

Journal of Virology　Vol. 91　No. 21　2017/11/01　Research paper (scientific journal)

Entirely plasmid-based reverse genetics system for rotaviruses

Yuta Kanai, Satoshi Komoto, Takahiro Kawagishi, Ryotaro Nouda, Naoko Nagasawa, Misa Onishi, Yoshiharu Matsuura, Koki Taniguchi, Takeshi Kobayashi

Proceedings of the National Academy of Sciences of the United States of America　Vol. 114　No. 9　p. 2343-2348　2017/02/28　Research paper (scientific journal)

[A plasmid-based reverse genetics system for rotaviruses].

Yuta Kanai, Takeshi Kobayashi

Uirusu　Vol. 67　No. 2　p. 99-110　2017

Reverse Genetics for Fusogenic Bat-Borne Orthoreovirus Associated with Acute Respiratory Tract Infections in Humans: Role of Outer Capsid Protein σC in Viral Replication and Pathogenesis

Takahiro Kawagishi, Yuta Kanai, Hideki Tani, Masayuki Shimojima, Masayuki Saijo, Yoshiharu Matsuura, Takeshi Kobayashi

PLoS Pathogens　Vol. 12　No. 2　2016/02　Research paper (scientific journal)

Immunogenicity of recombinant VP2 proteins of all nine serotypes of African horse sickness virus

Yuta Kanai, Piet A. van Rijn, Mieke Maris-Veldhuis, Yuki Kaname, T. N. Athmaram, Polly Roy

Vaccine　Vol. 32　No. 39　p. 4932-4937　2014/09/03　Research paper (scientific journal)

Imported case of acute respiratory tract infection associated with a member of species Nelson Bay orthoreovirus

Atsushi Yamanaka, Akira Iwakiri, Tomoki Yoshikawa, Kouji Sakai, Harpal Singh, Daisuke Himeji, Ikuo Kikuchi, Akira Ueda, Seigo Yamamoto, Miho Miura, Yoko Shioyama, Kimiko Kawano, Tokiko Nagaishi, Minako Saito, Masumi Minomo, Naoyasu Iwamoto, Yoshio Hidaka, Hirotoshi Sohma, Takeshi Kobayashi, Yuta Kanai, Takehiro Kawagishi, Noriyo Nagata, Shuetsu Fukushi, Tetsuya Mizutani, Hideki Tani, Satoshi Taniguchi, Aiko Fukuma, Masayuki Shimojima, Ichiro Kurane, Tsutomu Kageyama, Takato Odagiri, Masayuki Saijo, Shigeru Morikawa

PLoS ONE　Vol. 9　No. 3　2014/03/25　Research paper (scientific journal)

Recovery of African horse sickness virus from synthetic RNA

Yuuki Kaname, Cristina C.P. Celma, Yuta Kanai, Polly Roy

Journal of General Virology　Vol. 94　No. PART10　p. 2259-2265　2013/10　Research paper (scientific journal)

Multiple large foreign protein expression by a single recombinant baculovirus: A system for production of multivalent vaccines

Yuta Kanai, T. N. Athmaram, Meredith Stewart, Polly Roy

Protein Expression and Purification　Vol. 91　No. 1　p. 77-84　2013　Research paper (scientific journal)

Reliability of a Newly-Developed Immunochromatography Diagnostic Kit for Pandemic Influenza A/H1N1pdm Virus: Implications for Drug Administration

Tadahiro Sasaki, Ritsuko Kubota-Koketsu, Michihiro Takei, Tatsuo Hagihara, Shinichi Iwamoto, Takuya Murao, Kazuo Sawami, Daizou Fukae, Masahiro Nakamura, Eiichi Nagata, Akira Kawakami, Yuko Mitsubayashi, Masafumi Ohno, Yasuo Uehara, Takashi Fukukawa, Yuta Kanai, Mieko Kosaka, Kazuyoshi Ikuta

PLoS ONE　Vol. 7　No. 11　2012/11/30　Research paper (scientific journal)

Hepatitis e virus in Norway rats (Rattus norvegicus) captured around a pig farm

Yuta Kanai, Satoshi Miyasaka, Sachiko Uyama, Sachiyo Kawami, Yuko Kato-Mori, Muneo Tsujikawa, Mikihiro Yunoki, Shoko Nishiyama, Kazuyoshi Ikuta, Katsuro Hagiwara

BMC Research Notes　Vol. 5　p. 4-4　2012　Research paper (scientific journal)

Neutralization titers against influenza A (H3N2) and influenza B viruses among a non-vaccinated population from Thailand

Napasawan Boonsathorn, Yuta Kanai, Jiraporn Punjumpa, Guirong Bai, Malinee Chittaganpitch, Udom Petphuwadee, Wipawee Jampangern, Pathom Sawanpanyalert, Kazuyoshi Ikuta, Chai Teerasut

Southeast Asian Journal of Tropical Medicine and Public Health　Vol. 43　No. 3　p. 674-679　2012　Research paper (scientific journal)

Fermentation metabolites from Lactobacillus gasseri and Propionibacterium freudenreichii exert bacteriocidal effects in mice.

Yuko Kato-Mori, Takenori Orihashi, Yuta Kanai, Michiko Sato, Kenji Sera, Katsuro Hagiwara

Journal of medicinal food　Vol. 13　No. 6　p. 1460-1467　2010/12　Research paper (scientific journal)

Distinct propagation efficiencies of H5N1 influenza virus Thai isolates in newly established murine respiratory region-derived cell clones

Yuta Kanai, Malinee Chittaganpitch, Izuru Nakamura, Gui Mei Li, Gui Rong Bai, Yong Gang Li, Kazuyoshi Ikuta, Pathom Sawanpanyalert

Virus Research　Vol. 153　No. 2　p. 218-225　2010/11　Research paper (scientific journal)

The impact of antigenic drift of influenza A virus on human herd immunity: Sero-epidemiological study of H1N1 in healthy Thai population in 2009

Yuta Kanai, Naphatsawan Boonsathorn, Malinee Chittaganpitch, Guirong Bai, Yonggang Li, Tetsuo Kase, Kazuo Takahashi, Yoshinobu Okuno, Wipawee Jampangern, Kazuyoshi Ikuta, Pathom Sawanpanyalert

Vaccine　Vol. 28　No. 33　p. 5437-5444　2010/07　Research paper (scientific journal)

Infectious prion protein in the filtrate even after 15nm filtration

Mikihiro Yunoki, Hiroyuki Tanaka, Takeru Urayama, Yuta Kanai, Aya Nishida, Maki Yoshikawa, Yuji Ohkubo, Yoshiyasu Kawabata, Katsuro Hagiwara, Kazuyoshi Ikuta

Biologicals　Vol. 38　No. 2　p. 311-313　2010/03　Research paper (scientific journal)

Long-term shedding of hepatitis E virus in the feces of pigs infected naturally, born to sows with and without maternal antibodies

Yuta Kanai, Muneo Tsujikawa, Mikihiro Yunoki, Shoko Nishiyama, Kazuyoshi Ikuta, Katsuro Hagiwara

Journal of Medical Virology　Vol. 82　No. 1　p. 69-76　2010/01　Research paper (scientific journal)

Research Characterization of H5N1 influenza viruses isolated from humans in vitro

Yong Gang Li, Malinee Chittaganpitch, Sunthareeya Waicharoen, Yuta Kanai, Gui Rong Bai, Masanori Kameoka, Naokazu Takeda, Kazuyoshi Ikuta, Pathom Sawanpanyalert

Virology Journal　Vol. 7　No. 1　p. 112-112　2010　Research paper (scientific journal)

Amantadine- and oseltamivir-resistant variants of influenza A viruses in Thailand

Gui Rong Bai, Malinee Chittaganpitch, Yuta Kanai, Yong Gang Li, Wattana Auwanit, Kazuyoshi Ikuta, Pathom Sawanpanyalert

Biochemical and Biophysical Research Communications　Vol. 390　No. 3　p. 897-901　2009/12/18　Research paper (scientific journal)

The use of tetracycline in anthelmintic baits to assess baiting rate and drug efficacy against Echinococcus multilocularis in foxes.

Takashi Inoue, Nariaki Nonaka, Yuta Kanai, Takashi Iwaki, Masao Kamiya, Yuzaburo Oku

Veterinary parasitology　Vol. 150　No. 1-2　p. 88-96　2007/11/30

Epidemiology, histopathology, and muscle distribution of Trichinella T9 in feral raccoons (Procyon lotor) and wildlife of Japan.

Tomoko Kobayashi, Yuta Kanai, Yoko Ono, Yohei Matoba, Kazuo Suzuki, Minoru Okamoto, Hiroyuki Taniyama, Kinpei Yagi, Yuzaburo Oku, Ken Katakura, Mitsuhiko Asakawa

Parasitology research　Vol. 100　No. 6　p. 1287-91　2007/05

Epizootiological survey of Trichinella spp. infection in carnivores, rodents and insectivores in Hokkaido, Japan.

Yuta Kanai, Takashi Inoue, Tsutomu Mano, Nariaki Nonaka, Ken Katakura, Yuzaburo Oku

The Japanese journal of veterinary research　Vol. 54　No. 4　p. 175-82　2007/02

Trichinella nativa and Trichinella T9 in the Hokkaido island, Japan.

Yuta Kanai, Nariaki Nonaka, Ken Katakura, Yuzaburo Oku

Parasitology international　Vol. 55　No. 4　p. 313-5　2006/12

国内のウサギの胃に寄生するObeliscoides(Graybill,1923)属線虫の遺伝子学的検討

福本 真一郎, 小浜 菜美子, 六田 衣里, 福本 真也, 望月 里衣子, 阿部 愼太郎, 倉石 武, 服部 正策, 金井 祐太, 野中 成晃, 奥 祐三郎

日本獣医学会学術集会講演要旨集　Vol. 142回　p. 64-64　2006/08

Development of Taenia saginata asiatica metacestodes in SCID mice and its infectivity in human and alternative definitive hosts

S. L. Chang, N. Nonaka, M. Kamiya, Y. Kanai, H. K. Ooi, W. C. Chung, Y. Oku

Parasitology Research　Vol. 96　No. 2　p. 95-101　2005/05　Research paper (scientific journal)