In vitro screening of chemically synthesized dipeptide-antisense oligonucleotide conjugates to identify ligand molecules enhancing their activity

Takashi Osawa, Ryosuke Kita, Yuuya Kasahara, Harumi Yamaguma, Taisuke Nakayama, Haruhiko Kamada, Satoshi Obika

Bioorganic and Medicinal Chemistry　Vol. 110　p. 117814-117814　2024/08　Research paper (scientific journal)

Separation and Characterization of Therapeutic Oligonucleotide Isomer Impurities by Cyclic Ion Mobility Mass Spectrometry

Shogo Omuro, Takao Yamaguchi, Taiji Kawase, Kenji Hirose, Tokuyuki Yoshida, Takao Inoue, Satoshi Obika

Journal of the American Society for Mass Spectrometry　2024/07/31　Research paper (scientific journal)

Lantern-type G-quadruplex fluorescent sensors for detecting divalent metal ions

Tomoko Wariishi, Yuka Kataoka, Tomoaki Nakamura, Yuuya Kasahara, Masataka Kuroda, Satoshi Obika, Masayasu Kuwahara

Analytical Biochemistry　Vol. 690　p. 115525-115525　2024/07　Research paper (scientific journal)

Introduction of sugar-modified nucleotides into CpG-containing antisense oligonucleotides inhibits TLR9 activation

Tokuyuki Yoshida, Tomoko Hagihara, Yasunori Uchida, Yoshiyuki Horiuchi, Kiyomi Sasaki, Takenori Yamamoto, Takuma Yamashita, Yukihiro Goda, Yoshiro Saito, Takao Yamaguchi, Satoshi Obika, Seiji Yamamoto, Takao Inoue

Scientific Reports　Vol. 14　No. 1　2024/05/21　Research paper (scientific journal)

A novel transient receptor potential C3/C6 selective activator induces the cellular uptake of antisense oligonucleotides

Hiroto Kohashi, Ryu Nagata, Yusuke Tamenori, Tomorrow Amatani, Yoshifumi Ueda, Yasuo Mori, Yuuya Kasahara, Satoshi Obika, Masahito Shimojo

Nucleic Acids Research　Vol. 52　No. 9　p. 4784-4798　2024/04/16　Research paper (scientific journal)

Separation of the diastereomers of phosphorothioated siRNAs by anion-exchange chromatography under non-denaturing conditions

Hiroyuki Togawa, Takashi Okubo, Kazuki Horiuchi, Takao Yamaguchi, Elisa Tomita-Sudo, Tomoka Akita, Junji Kawakami, Satoshi Obika

Journal of Chromatography A　Vol. 1721　p. 464847-464847　2024/04　Research paper (scientific journal)

Light-controllable cell-membrane disturbance for intracellular delivery.

Wenting Huo, Koji Miki, Huiying Mu, Takashi Osawa, Harumi Yamaguma, Yuuya Kasahara, Satoshi Obika, Yoshimasa Kawaguchi, Hisaaki Hirose, Shiroh Futaki, Yusuke Miyazaki, Wataru Shinoda, Shuji Akai, Kouichi Ohe

Journal of materials chemistry. B　Vol. 12　No. 17　p. 4138-4147　2024/03/08　Research paper (scientific journal)

Favorable efficacy and reduced acute neurotoxicity by antisense oligonucleotides with 2′,4′-BNA/LNA with 9-(aminoethoxy)phenoxazine

Taiki Matsubayashi, Kotaro Yoshioka, Su Su Lei Mon, Maho Katsuyama, Chunyan Jia, Takao Yamaguchi, Rintaro Iwata Hara, Tetsuya Nagata, Osamu Nakagawa, Satoshi Obika, Takanori Yokota

Molecular Therapy - Nucleic Acids　Vol. 35　No. 2　p. 102161-102161　2024/03　Research paper (scientific journal)

Hydrophobicity Tuning of Cationic Polyaspartamide Derivatives for Enhanced Antisense Oligonucleotide Delivery.

Jongmin Yum, Fadlina Aulia, Keisuke Kamiya, Mao Hori, Nan Qiao, Beob Soo Kim, Mitsuru Naito, Satomi Ogura, Tetsuya Nagata, Takanori Yokota, Satoshi Uchida, Satoshi Obika, Hyun Jin Kim, Kanjiro Miyata

Bioconjugate chemistry　Vol. 35　No. 2　p. 125-131　2024/01/30　Research paper (scientific journal)

Synthesis of coumarin-conjugated oligonucleotides via knoevenagel condensation to prepare an oligonucleotide library

Takashi Osawa, Satoshi Obika

Chemical and Pharmaceutical Bulletin　Vol. 72　No. 2　p. 143-148　2024　Research paper (scientific journal)

Editorial: Nucleosides, nucleotides and nucleic acids: chemistry and biology

Leandro Soter de Mariz e Miranda, Raoni Schroeder Borges Gonçalves, Jacques Uziel, Satoshi Obika, Nadege Lubin-Germain

Frontiers in Chemistry　Vol. 12　2024　Research paper (scientific journal)

Splice-switching antisense oligonucleotide controlling tumor suppressor REST is a novel therapeutic medicine for neuroendocrine cancer

Keishiro Mishima, Satoshi Obika, Masahito Shimojo

Molecular Therapy Nucleic Acids　2024　Research paper (scientific journal)

Preclinical toxicological assessment of amido-bridged nucleic acid-modified antisense oligonucleotides targeting synaptotagmin XIII for intra-abdominal treatment of peritoneal metastasis of gastric cancer

Mitsuro Kanda, Nao Takano, Hiroshi Miyauchi, Kohei Ueda, Masaaki Mizuno, Yuuya Kasahara, Yasuhiro Kodera, Satoshi Obika

Gastric Cancer　2024　Research paper (scientific journal)

THF peroxide as a factor in generating desulphurised products from the solid-phase synthesis of phosphorothioate-modified oligonucleotides

Qin Ren, Takashi Osawa, Michiaki Tatsuno, Satoshi Obika

RSC Advances　Vol. 14　No. 30　p. 21590-21596　2024　Research paper (scientific journal)

Polyamines promote xenobiotic nucleic acid synthesis by modified thermophilic polymerase mutants

Hidekazu Hoshino, Yuuya Kasahara, Satoshi Obika

RSC Chemical Biology　Vol. 5　No. 5　p. 467-472　2024　Research paper (scientific journal)

Development of simple purification method for oligonucleotides synthesized using phosphoramidite for 5′-end modification as capping reagent

Qin Ren, Takashi Osawa, Satoshi Obika

Tetrahedron　Vol. 150　p. 133774-133774　2024/01　Research paper (scientific journal)

Synthesis, Duplex-Forming Ability, and Enzymatic Stability of Oligonucleotides Modified with Amide-Linked Dinucleotides Containing a 3',4'-Tetrahydropyran-Bridged Nucleic Acid.

Takashi Osawa, Taiki Akino, Satoshi Obika

The Journal of organic chemistry　Vol. 89　No. 1　p. 269-280　2023/12/08　Research paper (scientific journal)

Systematic Analysis of 2′-O-Alkyl Modified Analogs for Enzymatic Synthesis and Their Oligonucleotide Properties

Kenta Ishida, Yuuya Kasahara, Hidekazu Hoshino, Takumi Okuda, Satoshi Obika

Molecules　Vol. 28　No. 23　p. 7911-7911　2023/12/02　Research paper (scientific journal)

Post-Synthetic Nucleobase Modification of Oligodeoxynucleotides by Sonogashira Coupling and Influence of Alkynyl Modifications on the Duplex-Forming Ability.

Atsushi Mikami, Shohei Mori, Takashi Osawa, Satoshi Obika

Chemistry (Weinheim an der Bergstrasse, Germany)　Vol. 29　No. 63　2023/11/13　Research paper (scientific journal)

Mechanism of the extremely high duplex-forming ability of oligonucleotides modified with N-tert-butylguanidine- or N-tert-butyl-N′- methylguanidine-bridged nucleic acids

Takao Yamaguchi, Naohiro Horie, Hiroshi Aoyama, Shinji Kumagai, Satoshi Obika

Nucleic Acids Research　2023/08/25　Research paper (scientific journal)

Applicability of supercritical fluid chromatography for oligonucleotide analysis: A proof-of-concept study

Momoka Hayashida, Risa Suzuki, Shinnosuke Horie, Junichi Masuda, Takao Yamaguchi, Satoshi Obika

Journal of Chromatography A　p. 464333-464333　2023/08　Research paper (scientific journal)

Inhibition of microRNA-33b in humanized mice ameliorates nonalcoholic steatohepatitis.

Sawa Miyagawa, Takahiro Horie, Tomohiro Nishino, Satoshi Koyama, Toshimitsu Watanabe, Osamu Baba, Tomohiro Yamasaki, Naoya Sowa, Chiharu Otani, Kazuki Matsushita, Hidenori Kojima, Masahiro Kimura, Yasuhiro Nakashima, Satoshi Obika, Yuuya Kasahara, Jun Kotera, Kozo Oka, Ryo Fujita, Takashi Sasaki, Akihiro Takemiya, Koji Hasegawa, Takeshi Kimura, Koh Ono

Life science alliance　Vol. 6　No. 8　2023/08　Research paper (scientific journal)

Novel strategy of liver cancer treatment with modified antisense oligonucleotides targeting human vasohibin-2.

Sachiko Horie, Yasuhiro Suzuki, Tsuyoshi Yamamoto, Satoshi Obika, Kohta Mohri, Chizuru Kiyota, Qin Ren, Shota Warashina, Yasuhiro Wada, Yasuyoshi Watanabe, Hidefumi Mukai, Yasufumi Sato

Cancer science　2023/07/10　Research paper (scientific journal)

Synthesis and properties of a novel modified nucleic acid, 2′-N-methanesulfonyl-2′-amino-locked nucleic acid

Hiroaki Sawamoto, Takashi Sasaki, Tomo Takegawa-Araki, Masayuki Utsugi, Hiroyuki Furukawa, Yoko Hirakawa, Fumiko Yamairi, Takashi Kurita, Karin Murahashi, Katsuya Yamada, Tetsuya Ohta, Shinji Kumagai, Akihiro Takemiya, Satoshi Obika, Jun Kotera

Bioorganic & Medicinal Chemistry Letters　Vol. 88　p. 129289-129289　2023/05　Research paper (scientific journal)

Drug Metabolism and Pharmacokinetics of Antisense Oligonucleotide Therapeutics: Typical Profiles, Evaluation Approaches, and Points to Consider Compared with Small Molecule Drugs

Hideo Takakusa, Norihiko Iwazaki, Makiya Nishikawa, Tokuyuki Yoshida, Satoshi Obika, Takao Inoue

Nucleic Acid Therapeutics　Vol. 33　No. 2　p. 83-94　2023/04/01　Research paper (scientific journal)

Synthesis of multivalent fatty acid-conjugated antisense oligonucleotides: Cell internalization, physical properties, and in vitro and in vivo activities

Yuya Tanaka, Yurika Tanioku, Taisuke Nakayama, Kotomi Aso, Takao Yamaguchi, Haruhiko Kamada, Satoshi Obika

Bioorganic and Medicinal Chemistry　Vol. 81　p. 117192-117192　2023/03　Research paper (scientific journal)

An antisense amido-bridged nucleic acid gapmer oligonucleotide targeting SRRM4 alters REST splicing and exhibits anti-tumor effects in small cell lung cancer and prostate cancer cells.

Misa Yoshida, Chihiro Oda, Keishiro Mishima, Itsuki Tsuji, Satoshi Obika, Masahito Shimojo

Cancer cell international　Vol. 23　No. 1　p. 8-8　2023/01/17　Research paper (scientific journal)

2′-N-Alkylaminocarbonyl-2′-amino-LNA: Synthesis, duplex stability, nuclease resistance, and in vitro anti-microRNA activity

Tomo Takegawa-Araki, Kai Yasukawa, Norihiko Iwazaki, Masayuki Utsugi, Hiroyuki Furukawa, Shinji Kumagai, Hiroaki Sawamoto, Satoshi Obika

Bioorganic & Medicinal Chemistry　Vol. 78　p. 117148-117148　2023/01　Research paper (scientific journal)

Development of 8–17 XNAzymes that are functional in cells

Kosuke Chiba, Takao Yamaguchi, Satoshi Obika

Chemical Science　Vol. 14　No. 28　p. 7620-7629　2023　Research paper (scientific journal)

Synthesis and biophysical properties of tetravalent PEG-conjugated antisense oligonucleotide

Taslima Rahman Chowdhury, Tahia Taufiq, Kenta Ishida, Md Ariful Islam, Yuuya Kasahara, Takashi Osawa, Satoshi Obika

Bioorganic and Medicinal Chemistry　Vol. 78　p. 117149-117149　2023/01　Research paper (scientific journal)

Retention behavior of short double-stranded oligonucleotide and its potential impurities by anion-exchange chromatography under non-denaturing conditions

Hiroyuki Togawa, Takashi Okubo, Yumi Nonaka, Takao Yamaguchi, Satoshi Obika

Journal of Chromatography A　p. 463808-463808　2023/01　Research paper (scientific journal)

Synthesis and Properties of Nucleobase-Sugar Dual Modified Nucleic Acids: 2′-OMe-RNA and scpBNA Bearing a 5-Hydroxycytosine Nucleobase

Yota Sakurai, Takao Yamaguchi, Tokuyuki Yoshida, Masahiko Horiba, Takao Inoue, Satoshi Obika

The Journal of Organic Chemistry　2022/12/15　Research paper (scientific journal)

Development of Phosphoramidite Reagents for the Synthesis of Base-Labile Oligonucleotides Modified with a Linear Aminoalkyl and Amino-PEG Linker at the 3′-End

Takashi Osawa, Qin Ren, Satoshi Obika

Molecules　Vol. 27　No. 23　p. 8501-8501　2022/12/03　Research paper (scientific journal)

Synthesis and physical and biological properties of 1,3-diaza-2-oxophenoxazine-conjugated oligonucleotides.

Ryohei Yamaji, Osamu Nakagawa, Yuki Kishimoto, Akane Fujii, Tomoki Matsumura, Taisuke Nakayama, Haruhiko Kamada, Takashi Osawa, Takao Yamaguchi, Satoshi Obika

Bioorganic & medicinal chemistry　Vol. 72　p. 116972-116972　2022/10/15　Research paper (scientific journal)

Identification of nucleobase chemical modifications that reduce the hepatotoxicity of gapmer antisense oligonucleotides

Tokuyuki Yoshida, Kunihiko Morihiro, Yuki Naito, Atsushi Mikami, Yuuya Kasahara, Takao Inoue, Satoshi Obika

Nucleic Acids Research　Vol. 50　No. 13　p. 7224-7234　2022/07/08　Research paper (scientific journal)

Inhibition of microRNA-33b specifically ameliorates abdominal aortic aneurysm formation via suppression of inflammatory pathways

Tomohiro Yamasaki, Takahiro Horie, Satoshi Koyama, Tetsushi Nakao, Osamu Baba, Masahiro Kimura, Naoya Sowa, Kazuhisa Sakamoto, Kazuhiro Yamazaki, Satoshi Obika, Yuuya Kasahara, Jun Kotera, Kozo Oka, Ryo Fujita, Takashi Sasaki, Akihiro Takemiya, Koji Hasegawa, Kenji Minatoya, Takeshi Kimura, Koh Ono

SCIENTIFIC REPORTS　Vol. 12　No. 1　2022/07　Research paper (scientific journal)

Altered Biodistribution and Hepatic Safety Profile of a Gapmer Antisense Oligonucleotide Bearing Guanidine-Bridged Nucleic Acids

Takashi Sasaki, Yoko Hirakawa, Fumiko Yamairi, Takashi Kurita, Karin Murahashi, Hirokazu Nishimura, Norihiko Iwazaki, Hidenori Yasuhara, Takashi Tateoka, Tetsuya Ohta, Satoshi Obika, Jun Kotera

Nucleic Acid Therapeutics　Vol. 32　No. 3　p. 177-184　2022/06/01　Research paper (scientific journal)

Physicochemical property evaluation of modified oligonucleotides by traveling‐wave ion mobility mass spectrometry

Shogo Omuro, Takao Yamaguchi, Taiji Kawase, Maki Terasaki, Kenji Hirose, Satoshi Obika

Rapid Communications in Mass Spectrometry　Vol. 36　No. 10　2022/05/30　Research paper (scientific journal)

Synthesis of Sugar and Nucleoside Analogs and Evaluation of Their Anticancer and Analgesic Potentials.

Fahad Hussain, Fahad Imtiaz Rahman, Poushali Saha, Atsushi Mikami, Takashi Osawa, Satoshi Obika, S M Abdur Rahman

Molecules (Basel, Switzerland)　Vol. 27　No. 11　p. 3499-3499　2022/05/29　Research paper (scientific journal)

Administration of Gapmer-type Antisense Oligonucleotides Targeting γ-Glutamylcyclotransferase Suppresses the Growth of A549 Lung Cancer Xenografts.

Hiromi Ii, Yuuya Kasahara, Harumi Yamaguma, Susumu Kageyama, Akihiro Kawauchi, Satoshi Obika, Susumu Nakata

Anticancer research　Vol. 42　No. 3　p. 1221-1227　2022/03　Research paper (scientific journal)

Structure–Activity Relationships of Anti-microRNA Oligonucleotides Containing Cationic Guanidine-Modified Nucleic Acids

Tomo Takegawa-Araki, Shinji Kumagai, Kai Yasukawa, Masataka Kuroda, Takashi Sasaki, Satoshi Obika

Journal of Medicinal Chemistry　2022/02/10　Research paper (scientific journal)

Parallel synthesis of oligonucleotides containing N-acyl amino-LNA and their therapeutic effects as anti-microRNAs

Tomo Takegawa-Araki, Kai Yasukawa, Norihiko Iwazaki, Hideto Maruyama, Hiroyuki Furukawa, Hiroaki Sawamoto, Satoshi Obika

Organic & Biomolecular Chemistry　2022　Research paper (scientific journal)

Synthesis, Duplex-Forming Ability, and Nuclease Resistance of Oligonucleotides Containing a Thymidine Derivative with a 1-Oxaspiro[4.5]decane Skeleton.

Takashi Osawa, Natsumi Yano, Hiroshi Aoyama, Satoshi Obika

Chemical & pharmaceutical bulletin　Vol. 70　No. 10　p. 699-706　2022　Research paper (scientific journal)

Reduction of Off-Target Effects of Gapmer Antisense Oligonucleotides by Oligonucleotide Extension

Hidenori Yasuhara, Tokuyuki Yoshida, Kiyomi Sasaki, Satoshi Obika, Takao Inoue

MOLECULAR DIAGNOSIS & THERAPY　Vol. 26　No. 1　p. 117-127　2022/01　Research paper (scientific journal)

Conjugation of oligonucleotides with activated carbamate reagents prepared by the Ugi reaction for oligonucleotide library synthesis

Ryosuke Kita, Takashi Osawa, Satoshi Obika

RSC Chemical Biology　Vol. 3　No. 6　p. 728-738　2022　Research paper (scientific journal)

Drug discovery and development scheme for liver-targeting bridged nucleic acid antisense oligonucleotides

Fumito Wada, Tsuyoshi Yamamoto, Tadayuki Kobayashi, Keisuke Tachibana, Kosuke Ramon Ito, Mayumi Hamasaki, Yukina Kayaba, Chisato Terada, Asako Yamayoshi, Satoshi Obika, Mariko Harada-Shiba

MOLECULAR THERAPY-NUCLEIC ACIDS　Vol. 26　p. 957-969　2021/12　Research paper (scientific journal)

Identification of Potent and Selective Inhibitors of Fat Mass Obesity-Associated Protein Using a Fragment-Merging Approach

Muthuraj Prakash, Yukihiro Itoh, Yoshie Fujiwara, Yukari Takahashi, Yuri Takada, Paolo Mellini, Elghareeb E. Elboray, Mitsuhiro Terao, Yasunobu Yamashita, Chika Yamamoto, Takao Yamaguchi, Masayuki Kotoku, Yuki Kitao, Ritesh Singh, Rohini Roy, Satoshi Obika, Makoto Oba, Dan Ohtan Wang, Takayoshi Suzuki

JOURNAL OF MEDICINAL CHEMISTRY　Vol. 64　No. 21　p. 15810-15824　2021/11　Research paper (scientific journal)

Synthesis and duplex-forming ability of oligonucleotides modified with 4′-C,5′-C-methylene-bridged nucleic acid (4′,5′-BNA)

Takao Yamaguchi, Chika Yamamoto, Masahiko Horiba, Hiroshi Aoyama, Satoshi Obika

Bioorganic & Medicinal Chemistry　Vol. 46　p. 116359-116359　2021/09　Research paper (scientific journal)

Programmed Instability of Ligand Conjugation Manifold for Efficient Hepatocyte Delivery of Therapeutic Oligonucleotides

Chisato Terada, Fumito Wada, Mei Uchida, Yukari Yasutomi, Kaho Oh, Seiya Kawamoto, Yukina Kayaba, Asako Yamayoshi, Mariko Harada-Shiba, Satoshi Obika, Tsuyoshi Yamamoto

Nucleic Acid Therapeutics　Vol. 31　No. 6　p. 404-416　2021/08/31　Research paper (scientific journal)

Title: Gene transfer by pyro-drive jet injector is a novel therapeutic approach for muscle diseases

Takafumi Nakae, Masanori Obana, Takahiro Maeda, Akari Ikeda, Hiroshi Miyazaki, Shota Tanaka, Makiko Maeda, Kunihiko Yamashita, Kazuhiro Terai, Satoshi Obika, Yasushi Fujio

Gene　Vol. 788　p. 145664-145664　2021/07　Research paper (scientific journal)

Hybrid-Type SELEX for the Selection of Artificial Nucleic Acid Aptamers Exhibiting Cell Internalization Activity

Hiro Uemachi, Yuuya Kasahara, Keisuke Tanaka, Takumi Okuda, Yoshihiro Yoneda, Satoshi Obika

Pharmaceutics　Vol. 13　No. 6　p. 888-888　2021/06/15　Research paper (scientific journal)

Highly Potent GalNAc-Conjugated Tiny LNA Anti-miRNA-122 Antisense Oligonucleotides

Tsuyoshi Yamamoto, Yahiro Mukai, Fumito Wada, Chisato Terada, Yukina Kayaba, Kaho Oh, Asako Yamayoshi, Satoshi Obika, Mariko Harada–Shiba

Pharmaceutics　Vol. 13　No. 6　p. 817-817　2021/05/31　Research paper (scientific journal)

Considerations of the Japanese Research Working Group for the ICH S6 & Related Issues Regarding Nonclinical Safety Assessments of Oligonucleotide Therapeutics: Comparison with Those of Biopharmaceuticals

Yoko Hirabayashi, Kazushige Maki, Kiyoshi Kinoshita, Takahiro Nakazawa, Satoshi Obika, Misaki Naota, Kazuto Watanabe, Mutsumi Suzuki, Teruyo Arato, Aki Fujisaka, Osamu Fueki, Kosuke Ito, Hiroshi Onodera

Nucleic Acid Therapeutics　Vol. 31　No. 2　p. 114-125　2021/04/01　Research paper (scientific journal)

Design and In Vitro Evaluation of Splice-Switching Oligonucleotides Bearing Locked Nucleic Acids, Amido-Bridged Nucleic Acids, and Guanidine-Bridged Nucleic Acids

Takenori Shimo, Yusuke Nakatsuji, Keisuke Tachibana, Satoshi Obika

International Journal of Molecular Sciences　Vol. 22　No. 7　p. 3526-3526　2021/03/29　Research paper (scientific journal)

Synthesis and properties of oligonucleotides modified with an N-methylguanidine-bridged nucleic acid (GuNA[Me]) bearing adenine, guanine, or 5-methylcytosine nucleobases

Naohiro Horie, Takao Yamaguchi, Shinji Kumagai, Satoshi Obika

Beilstein Journal of Organic Chemistry　Vol. 17　p. 622-629　2021/03/04　Research paper (scientific journal)

2′,4′‐BNA/LNA with 9‐(2‐Aminoethoxy)‐1,3‐diaza‐2‐oxophenoxazine Efficiently Forms Duplexes and Has Enhanced Enzymatic Resistance**

Yuki Kishimoto, Osamu Nakagawa, Akane Fujii, Kotaro Yoshioka, Tetsuya Nagata, Takanori Yokota, Yoshiyuki Hari, Satoshi Obika

Chemistry – A European Journal　Vol. 27　No. 7　p. 2427-2438　2021/02/21　Research paper (scientific journal)

Crystallographic Structure of Novel Types of Ag I ‐Mediated Base Pairs in Non‐canonical DNA Duplex Containing 2′‐ O ,4′‐ C ‐Methylene Bridged Nucleic Acids

Osamu Nakagawa, Hiroshi Aoyama, Akane Fujii, Yuki Kishimoto, Satoshi Obika

Chemistry – A European Journal　Vol. 27　No. 11　p. 3842-3848　2021/02/19　Research paper (scientific journal)

Alpha‐synuclein dynamics in induced pluripotent stem cell‐derived dopaminergic neurons from a Parkinson’s disease patient ( PARK4 ) with SNCA triplication

Hayato Fukusumi, Kazuyuki Togo, Miho Sumida, Masayuki Nakamori, Satoshi Obika, Kousuke Baba, Tomoko Shofuda, Daisuke Ito, Hideyuki Okano, Hideki Mochizuki, Yonehiro Kanemura

FEBS Open Bio　Vol. 11　No. 2　p. 354-366　2021/02/05　Research paper (scientific journal)

A gapmer antisense oligonucleotide targeting SRRM4 is a novel therapeutic medicine for small cell lung cancer.

Masahito Shimojo, Satoshi Obika

CANCER SCIENCE　Vol. 112　p. 583-583　2021/02

Enhanced Duplex- and Triplex-forming Ability and Enzymatic Resistance of Oligodeoxynucleotides Modified by a Tricyclic Thymine Derivative

Yuki Kishimoto, Akane Fujii, Osamu Nakagawa, Satoshi Obika

Organic & Biomolecular Chemistry　Vol. 19　No. 37　p. 8063-8074　2021　Research paper (scientific journal)

DNA Polymerase Variants with High Processivity and Accuracy for Encoding and Decoding Locked Nucleic Acid Sequences

Hidekazu Hoshino, Yuuya Kasahara, Masayasu Kuwahara, Satoshi Obika

Journal of the American Chemical Society　Vol. 142　No. 51　p. 21530-21537　2020/12/23　Research paper (scientific journal)

Amido-Bridged Nucleic Acid-Modified Antisense Oligonucleotides Targeting SYT13 to Treat Peritoneal Metastasis of Gastric Cancer

Mitsuro Kanda, Yuuya Kasahara, Dai Shimizu, Takashi Miwa, Shinichi Umeda, Koichi Sawaki, Shunsuke Nakamura, Yasuhiro Kodera, Satoshi Obika

Molecular Therapy - Nucleic Acids　Vol. 22　p. 791-802　2020/12　Research paper (scientific journal)

Theoretical analyses and experimental validation of the effects caused by the fluorinated substituent modification of DNA

Jun Koseki, Masamitsu Konno, Ayumu Asai, Naohiro Horie, Kenta Tsunekuni, Koichi Kawamoto, Satoshi Obika, Yuichiro Doki, Masaki Mori, Hideshi Ishii

Scientific Reports　Vol. 10　No. 1　p. 1138-1138　2020/12　Research paper (scientific journal)

Synthesis and properties of GuNA purine/pyrimidine nucleosides and oligonucleotides

Kumagai, S., Sawamoto, H., Takegawa-Araki, T., Arai, Y., Yamakoshi, S., Yamada, K., Ohta, T., Kawanishi, E., Horie, N., Yamaguchi, T., Obika, S.

Organic and Biomolecular Chemistry　Vol. 18　No. 46　p. 9461-9472　2020/11　Research paper (scientific journal)

Base-modified aptamers obtained by cell-internalization SELEX facilitate cellular uptake of an antisense oligonucleotide

Keisuke Tanaka, Takumi Okuda, Yuuya Kasahara, Satoshi Obika

Molecular Therapy - Nucleic Acids　Vol. 23　p. 440-449　2020/11　Research paper (scientific journal)

Azobenzene-modified DNA aptamers evolved by capillary electrophoresis (CE)-SELEX method.

Kunihiko Morihiro, Osamu Hasegawa, Yuuya Kasahara, Shohei Mori, Tatsuro Kasai, Masayasu Kuwahara, Satoshi Obika

Bioorganic & medicinal chemistry letters　p. 127607-127607　2020/10/08　Research paper (scientific journal)

Discovery of cell-internalizing artificial nucleic acid aptamers for lung fibroblasts and targeted drug delivery.

Hiro Uemachi, Yuuya Kasahara, Keisuke Tanaka, Takumi Okuda, Yoshihiro Yoneda, Satoshi Obika

Bioorganic chemistry　Vol. 105　p. 104321-104321　2020/10/01　Research paper (scientific journal)

Effect of oligonucleotide structural difference on matrix‐assisted laser desorption/ionization in‐source decay in comparison with collision‐induced dissociation fragmentation

Satoshi Kimura, Satoshi Obika

Rapid Communications in Mass Spectrometry　Vol. 34　No. 16　2020/07/16　Research paper (scientific journal)

Synthesis and Evaluation of Artificial Nucleic Acid Bearing an Oxanorbornane Scaffold

Hibiki Komine, Shohei Mori, Kunihiko Morihiro, Kenta Ishida, Takumi Okuda, Yuuya Kasahara, Hiroshi Aoyama, Takao Yamaguchi, Satoshi Obika

Molecules　Vol. 25　No. 7　p. 1732-1732　2020/04/09　Research paper (scientific journal)

Oligonucleotides Containing Phenoxazine Artificial Nucleobases: Triplex‐Forming Abilities and Fluorescence Properties

Akane Fujii, Osamu Nakagawa, Yuki Kishimoto, Yusuke Nakatsuji, Natsumi Nozaki, Satoshi Obika

ChemBioChem　Vol. 21　No. 6　p. 860-864　2020/03/16　Research paper (scientific journal)

Synthesis and Properties of Oligonucleotides Having Ethynylphosphonate Linkages

Masahiko Horiba, Takao Yamaguchi, Satoshi Obika

The Journal of Organic Chemistry　Vol. 85　No. 4　p. 1794-1801　2020/02/21　Research paper (scientific journal)

Synthesis of selenomethylene-locked nucleic acids (SeLNA) nucleoside unit bearing an adenine base

Yoshihiro Moai, Hidekazu Hiroaki, Saoshi Obika, Tetsuya Kodama

Nucleosides, Nucleotides & Nucleic Acids　Vol. 39　No. 1-3　p. 131-140　2020/02/20　Research paper (scientific journal)

Nucleobase derivatives induce in‐source decay of oligonucleotides as new matrix‐assisted laser desorption/ionization matrices

Satoshi Kimura, Aki Fujisaka, Satoshi Obika

Rapid Communications in Mass Spectrometry　Vol. 34　No. 6　2020/02/07　Research paper (scientific journal)

Calcium-mediated in vitro transfection technique of oligonucleotides with broad chemical modification compatibility

Fumito Wada, Shin-ichiro Hori, Satoshi Obika, Tsuyoshi Yamamoto

Methods in Molecular Biology　Vol. 2176　p. 141-154　2020　Part of collection (book)

Different reactivity of Sp and Rp isomers of phosphorothioate-modified oligonucleotides in a duplex structure

Md Ariful Islam, Aki Fujisaka, Junji Kawakami, Takao Yamaguchi, Satoshi Obika

Bioorg. Med. Chem. Lett.　Vol. 30　No. 14　p. 127166-127166　2020　Research paper (scientific journal)

Inhibiting guanine oxidation and enhancing the excess-electron-transfer efficiency of a pyrene-modified oligonucleotide by introducing an electron-donating group on pyrene

Takumi Okuda, Yusuke Kawashima, Yuuya Kasahara, Tatsuya Takagi, Junpei Yamamoto, Shigenori Iwai, Satoshi Obika

Chemical Communications　Vol. 55　No. 93　p. 14062-14065　2019/12　Research paper (scientific journal)

Evaluation of off-target effects of gapmer antisense oligonucleotides using human cells.

Yoshida T, Naito Y, Yasuhara H, Sasaki K, Kawaji H, Kawai J, Naito M, Okuda H, Obika S, Inoue T

Genes to cells : devoted to molecular & cellular mechanisms　Vol. 24　No. 12　p. 827-835　2019/12　Research paper (scientific journal)

Synthesis of 2′-C,4′-C-Methyleneoxy-Bridged Thymidine Derivatives and Properties of Modified Oligonucleotides

Takashi Osawa, Han Kim, Misa Shoji, Miku Saijo, Masakazu Dohi, Yuta Ito, Satoshi Obika, Yoshiyuki Hari

The Journal of Organic Chemistry　Vol. 84　No. 21　p. 13336-13344　2019/11/01　Research paper (scientific journal)

Effect of modular conjugation strategy for N-acetylgalactosamine-targeted antisense oligonucleotides.

Yamamoto T, Sawamura M, Terada C, Kashiwada K, Wada F, Yamayoshi A, Obika S, Harada-Shiba M

Nucleosides, nucleotides & nucleic acids　Vol. 39　No. 1-3　p. 1-10　2019/10　Research paper (scientific journal)

Synthesis of Monovalent N-Acetylgalactosamine Phosphoramidite for Liver-Targeting Oligonucleotides

Yamamoto, T., Terada, C., Kashiwada, K., Yamayoshi, A., Harada-Shiba, M., Obika, S.

Current protocols in nucleic acid chemistry　Vol. 78　No. 1　2019/09　Research paper (scientific journal)

GREB1 induced by Wnt signaling promotes development of hepatoblastoma by suppressing TGFβ signaling.

Shinji Matsumoto, Taku Yamamichi, Koei Shinzawa, Yuuya Kasahara, Satoshi Nojima, Takahiro Kodama, Satoshi Obika, Tetsuo Takehara, Eiichi Morii, Hiroomi Okuyama, Akira Kikuchi

Nature communications　Vol. 10　No. 1　p. 3882-3882　2019/08/28

A Hydrogen Peroxide Activatable Gemcitabine Prodrug for the Selective Treatment of Pancreatic Ductal Adenocarcinoma.

Katsunori Matsushita, Takumi Okuda, Shohei Mori, Masamitsu Konno, Hidetoshi Eguchi, Ayumu Asai, Jun Koseki, Yoshifumi Iwagami, Daisaku Yamada, Hirofumi Akita, Tadafumi Asaoka, Takehiro Noda, Koichi Kawamoto, Kunihito Gotoh, Shogo Kobayashi, Yuuya Kasahara, Kunihiko Morihiro, Taroh Satoh, Yuichiro Doki, Masaki Mori, Hideshi Ishii, Satoshi Obika

ChemMedChem　Vol. 14　No. 15　p. 1384-1391　2019/08/06

Enhancement of exon skipping activity by reduction in the secondary structure content of LNA-based splice-switching oligonucleotides.

Shimo T, Tachibana K, Kawawaki Y, Watahiki Y, Ishigaki T, Nakatsuji Y, Hara T, Kawakami J, Obika S

Chem Commun (Camb)　Vol. 55　No. 48　p. 6850-6835　2019/06　Research paper (scientific journal)

Highly efficient silencing of microRNA by heteroduplex oligonucleotides.

Yoshioka K, Kunieda T, Asami Y, Guo H, Miyata H, Yoshida-Tanaka K, Sujino Y, Piao W, Kuwahara H, Nishina K, Hara RI, Nagata T, Wada T, Obika S, Yokota T

Nucleic acids research　Vol. 47　No. 14　p. 7321-7332　2019/06　Research paper (scientific journal)

A gapmer antisense oligonucleotide targeting SRRM4 is a novel therapeutic medicine for lung cancer.

Shimojo M, Kasahara Y, Inoue M, Tsunoda SI, Shudo Y, Kurata T, Obika S

Scientific reports　Vol. 9　No. 1　p. 7618-7618　2019/05　Research paper (scientific journal)

Amido-bridged nucleic acid (AmNA)-modified antisense oligonucleotides targeting α-synuclein as a novel therapy for Parkinson’s disease

Uehara, T., Choong, C.-J., Nakamori, M., Hayakawa, H., Nishiyama, K., Kasahara, Y., Baba, K., Nagata, T., Yokota, T., Tsuda, H., Obika, S., Mochizuki, H.

Scientific Reports　Vol. 9　No. 1　2019/05　Research paper (scientific journal)

Effective syntheses of 2′,4′-BNA NC monomers bearing adenine, guanine, thymine, and 5-methylcytosine, and the properties of oligonucleotides fully modified with 2′,4′-BNA NC

Aki Fujisaka, Yoshiyuki Hari, Hiroko Takuma, S.M. Abdur Rahman, Haruhisa Yoshikawa, Juanjuan Pang, Takeshi Imanishi, Satoshi Obika

Bioorganic and Medicinal Chemistry　Vol. 27　No. 8　p. 1728-1741　2019/04/15　Research paper (scientific journal)

Thioamide‐Bridged Nucleic Acid (thioAmNA) Containing Thymine or 2‐Thiothymine: Duplex‐Forming Ability, Base Discrimination, and Enzymatic Stability

Takaki Habuchi, Takao Yamaguchi, Satoshi Obika

ChemBioChem　Vol. 20　No. 8　p. 1060-1067　2019/04/15　Research paper (scientific journal)

Chemically Modified Antisense Oligonucleotide Against ARL4C Inhibits Primary and Metastatic Liver Tumor Growth.

Takeshi Harada, Shinji Matsumoto, Suguru Hirota, Hirokazu Kimura, Shinsuke Fujii, Yuuya Kasahara, Hidetoshi Gon, Toshihiko Yoshida, Tomoo Itoh, Naotsugu Haraguchi, Tsunekazu Mizushima, Takehiro Noda, Hidetoshi Eguchi, Satoshi Nojima, Eiichi Morii, Takumi Fukumoto, Satoshi Obika, Akira Kikuchi

Molecular cancer therapeutics　Vol. 18　No. 3　p. 602-612　2019/03　Research paper (scientific journal)

High-Contrast Facile Imaging with Target-Directing Fluorescent Molecular Rotors, the N3-Modified Thioflavin T Derivatives

Yuka Kataoka, Hiroto Fujita, Arina Afanaseva, Chioko Nagao, Kenji Mizuguchi, Yuuya Kasahara, Satoshi Obika, Masayasu Kuwahara

Biochemistry　Vol. 58　No. 6　p. 493-498　2019/02　Research paper (scientific journal)

Detection of esterase activity by chromogenic and fluorogenic probe based on an O-nitrobenzoxadiazole (O-NBD) unit

Okada, K., Yamaguchi, T., Dodo, K., Sodeoka, M., Obika, S.

Bioorganic and Medicinal Chemistry　Vol. 27　No. 7　p. 1444-1448　2019/02　Research paper (scientific journal)

Effective syntheses of 2′,4′-BNANC monomers bearing adenine, guanine, thymine, and 5-methylcytosine, and the properties of oligonucleotides fully modified with 2′,4′-BNANC

Aki Fujisaka, Yoshiyuki Hari, Hiroko Takuma, S.M. Abdur Rahman, Haruhisa Yoshikawa, Juanjuan Pang, Takeshi Imanishi, Satoshi Obika

Bioorganic & Medicinal Chemistry　Vol. 27　p. 1728-1741　2019/02　Research paper (scientific journal)

Hybridization and Mismatch Discrimination Abilities of 2′,4′-Bridged Nucleic Acids Bearing 2-Thiothymine or 2-Selenothymine Nucleobase

Takaki Habuchi, Takao Yamaguchi, Hiroshi Aoyama, Masahiko Horiba, Kosuke Ramon Ito, Satoshi Obika

The Journal of Organic Chemistry　Vol. 84　No. 3　p. 1430-1439　2019/02/01　Research paper (scientific journal)

Enhancement of exon skipping activity by reduction in the secondary structure content of LNA-based splice-switching oligonucleotides

Takenori Shimo, Keisuke Tachibana, Yuki Kawawaki, Yuuka Watahiki, Taku Ishigaki, Yusuke Nakatsuji, Takashi Hara, Junji Kawakami, Satoshi Obika

Chemical Communications　Vol. 55　p. 6850-6853　2019　Research paper (scientific journal)

Derivative Synthesis toward Enhancement of the Biophysical Properties of 2′,4′-Bridged Nucleic Acids

Takao Yamaguchi, Satoshi Obika

J. Synth. Org. Chem.　Vol. 77　No. 10　p. 994-1004　2019　Research paper (scientific journal)

Thymidine, 2-thiothymidine, and 2-selenothymidine: Comparison of mismatch discriminations

Habuchi, T., Yamaguchi, T., Obika, S.

Heterocycles　Vol. 98　No. 6　p. 832-837　2019　Research paper (scientific journal)

2′-C,4′-C-Ethyleneoxy-Bridged 2′-Deoxyribonucleic Acids (EoDNAs) with thymine nucleobases: Synthesis, duplex-forming ability, and enzymatic stability

Osawa, T., Obika, S., Hari, Y.

Methods in Molecular Biology　Vol. 1973　p. 59-89　2019　Research paper (scientific journal)

1,3,9-Triaza-2-oxophenoxazine: An Artificial Nucleobase Forming Highly Stable Self-Base Pairs with Three Ag^I Ions in a Duplex

Fujii A, Nakagawa O, Kishimoto Y, Okuda T, Nakatsuji Y, Nozaki N, Kasahara Y, Obika S

Chemistry - A European Journal (selected as Front Cover, Cover Profile and Hot Paper)　Vol. 25　No. 31　p. 7443-7448　2019　Research paper (scientific journal)

Cover Profile: 1,3,9‐Triaza‐2‐oxophenoxazine: An Artificial Nucleobase Forming Highly Stable Self‐Base Pairs with Three AgI Ions in a Duplex

Fujii A, Nakagawa O, Kishimoto Y, Okuda T, Nakatsuji Y, Nozaki N, Kasahara Y, Obika S

Chemistry - A European Journal　Vol. 25　No. 31　p. 7407-7448　2019　Research paper (scientific journal)

2',4'-bridged nucleic acids containing plural heteroatoms in the bridge moiety

Yoshiyuki Hari, Satoshi Obika

Synthesis of Therapeutic Oligonucleotides　p. 201-221　2018/12/08　Part of collection (book)

High-Contrast Facile Imaging with Target-Directing Fluorescent Molecular Rotors N3-Modified Thioflavin T Derivatives

Y. Kataoka, H. Fujita, A. Afanasyeva, C. Nagao, K. Mizuguchi, Y. Kasahara, S. Obika, M. Kuwahara

Biochemistry　Vol. 58　p. 493-498　2018/12　Research paper (scientific journal)

Development of a detection method for antisense oligonucleotides in mouse kidneys by matrix-assisted laser desorption/ionization imaging mass spectrometry.

Yokoi H, Kasahara Y, Obika S, Doi T, Kamada H

Rapid communications in mass spectrometry : RCM　Vol. 32　No. 23　p. 1984-1990　2018/12　Research paper (scientific journal)

2′‐O,4′‐C‐Methylene‐Bridged Nucleic Acids Stabilize Metal‐Mediated Base Pairing in a DNA Duplex

Osamu Nakagawa, Akane Fujii, Yuki Kishimoto, Yusuke Nakatsuji, Natsumi Nozaki, Satoshi Obika

ChemBioChem　2018/11/16　Research paper (scientific journal)

Front Cover: 2′‐O,4′‐C‐Methylene‐Bridged Nucleic Acids Stabilize Metal‐Mediated Base Pairing in a DNA Duplex (ChemBioChem 22/2018)

Osamu Nakagawa, Akane Fujii, Yuki Kishimoto, Yusuke Nakatsuji, Natsumi Nozaki, Satoshi Obika

ChemBioChem　2018/11/16　Research paper (scientific journal)

Correction: Development of oligonucleotide-based antagonists of Ebola virus protein 24 inhibiting its interaction with karyopherin alpha 1.

Tanaka K, Kasahara Y, Miyamoto Y, Okuda T, Kasai T, Onodera K, Kuwahara M, Oka M, Yoneda Y, Obika S

Organic & biomolecular chemistry　Vol. 16　No. 40　p. 7530-7530　2018/10/28　Research paper (scientific journal)

2′-O,4′-C-Methylene-bridged Nucleic Acids Stabilize Metal-mediated Base Pairing in A DNA Duplex

O. Nakagawa, A. Fujii, Y. Kishimoto, Y. Nakatsuji, N. Nozaki, S. Obika

ChemBioChem　Vol. 19　p. 2372-2379　2018/10　Research paper (scientific journal)

Facile synthesis and fundamental properties of an N-methylguanidine-bridged nucleic acid (GuNA[NMe]).

Horie N, Kumagai S, Kotobuki Y, Yamaguchi T, Obika S

Organic & biomolecular chemistry　Vol. 16　No. 35　p. 6531-6536　2018/09/21

Synthesis and properties of oligonucleotides containing 3'-O,4'-C-ethyleneoxy-bridged 5-methyluridines

Osawa T, Hitomi Y, Wakita S, Kim H, Dohi M, Horiba M, Ito Y, Obika S, Hari Y

Heterocycles　Vol. 97　No. 1　p. 306-313　2018/09　Research paper (scientific journal)

Facile Synthesis and Fundamental Properties of An N-Methylguanidine-Bridged Nucleic Acid (GuNA[NMe])

N. Horie, S. Kumagai, Y. Kotobuki, T. Yamaguchi, S. Obika

Organic & Biomolecular Chemistry　Vol. 16　p. 6531-6536　2018/08　Research paper (scientific journal)

Development of A Detection Method for Antisense Oligonucleotides in Mouse Kidneys by MALDI Imaging Mass Spectrometry

H. Yokoi, Y. Kasahara, S. Obika, T. Doi, H. Kamada

Rapid Communications in Mass Spectrometry　2018/08　Research paper (scientific journal)

Synthesis and biophysical properties of 5′-thio-2′,4′-BNA/LNA oligonucleotide

Md Ariful Islam, Aki Fujisaka, Shohei Mori, Kosuke Ramon Ito, Takao Yamaguchi, Satoshi Obika

Bioorganic and Medicinal Chemistry　Vol. 26　No. 12　p. 3634-3638　2018/07/23　Research paper (scientific journal)

Effective gene silencing activity of prodrug-type 2'-O-methyldithiomethyl siRNA compared with non-prodrug-type 2'-O-methyl siRNA.

Junsuke Hayashi, Misa Nishigaki, Yosuke Ochi, Shun-Ichi Wada, Fumito Wada, Osamu Nakagawa, Satoshi Obika, Mariko Harada-Shiba, Hidehito Urata

Bioorganic & medicinal chemistry letters　Vol. 28　No. 12　p. 2171-2174　2018/07/01　Research paper (scientific journal)

Generation of splice switching oligonucleotides targeting the Cockayne syndrome group B gene product in order to change the diseased cell state

Yooksil Sin, Futaba Makimura, Masafumi Saijo, Satoshi Obika

Biochemical and Biophysical Research Communications　Vol. 500　No. 2　p. 163-169　2018/06/02　Research paper (scientific journal)

Estimated number of off-target candidate sites for antisense oligonucleotides in human mRNA sequences

Tokuyuki Yoshida, Yuki Naito, Kiyomi Sasaki, Eriko Uchida, Yoji Sato, Mikihiko Naito, Toru Kawanishi, Satoshi Obika, Takao Inoue

Genes to Cells　Vol. 23　No. 6　p. 448-455　2018/06　Research paper (scientific journal)

Synthesis and Biophysical Properties of 5’-Thio-2’,4’-BNA/LNA Oligonucleotide

Md A. Islam, A. Fujisaka, S. Mori, K. R. Ito, T. Yamaguchi, S. Obika

Bioorganic & Medicinal Chemistry　Vol. 26　p. 3634-3638　2018/05　Research paper (scientific journal)

Development of Oligonucleotide-based Antagonists of Ebola Virus Protein 24 Inhibiting Its Interaction with Karyopherin Alpha 1

K. Tanaka, Y. Kasahara, Y. Miyamoto, T. Okuda, T. Kasai, K. Onodera, M. Kuwahara, M. Oka, Y. Yoneda, S. Obika

Organic & Biomolecular Chemistry　Vol. 16　p. 4456-4463　2018/05　Research paper (scientific journal)

Construction of a tri-chromatic reporter cell line for the rapid and simple screening of splice-switching oligonucleotides targeting DMD exon 51 using high content screening

Takenori Shimo, Keisuke Tachibana, Satoshi Obika

PLoS ONE　Vol. 13　No. 5　2018/05/01　Research paper (scientific journal)

Synthetic Method for 2′-Amino-LNA Bearing Any of the Four Nucleobases via a Transglycosylation Reaction

Hiroaki Sawamoto, Yuuki Arai, Shuhei Yamakoshi, Satoshi Obika, Eiji Kawanishi

Organic Letters　Vol. 20　No. 7　p. 1928-1931　2018/04/06　Research paper (scientific journal)

A novel human muscle cell model of Duchenne muscular dystrophy created by CRISPR/Cas9 and evaluation of antisense-mediated exon skipping

Takenori Shimo, Kana Hosoki, Yusuke Nakatsuji, Toshifumi Yokota, Satoshi Obika

Journal of Human Genetics　Vol. 63　No. 3　p. 365-375　2018/03　Research paper (scientific journal)

Cholesterol-GalNAc Dual Conjugation Strategy for Reducing Renal Distribution of Antisense Oligonucleotides.

Fumito Wada, Tsuyoshi Yamamoto, Tetsuya Ueda, Motoki Sawamura, Shunsuke Wada, Mariko Harada-Shiba, Satoshi Obika

Nucleic acid therapeutics　Vol. 28　No. 1　p. 50-57　2018/02　Research paper (scientific journal)

Preface

Obika, S., Sekine, M.

Synthesis of Therapeutic Oligonucleotides　2018　Research paper (scientific journal)

Hydrogen peroxide-Triggered gene silencing in mammalian cells through boronated antisense oligonucleotides

Shohei Mori, Kunihiko Morihiro, Takumi Okuda, Yuuya Kasahara, Satoshi Obika

Chemical Science　Vol. 9　No. 5　p. 1112-1118　2018　Research paper (scientific journal)

Development of oligonucleotide-based antagonists of Ebola virus protein 24 inhibiting its interaction with karyopherin alpha 1

Keisuke Tanaka, Yuuya Kasahara, Yoichi Miyamoto, Okuda Takumi, Tatsuro Kasai, Kentaro Onodera, Masayasu Kuwahara, Masahiro Oka, Yoshihiro Yoneda, Satoshi Obika

Organic and Biomolecular Chemistry　Vol. 16　No. 24　p. 4456-4463　2018　Research paper (scientific journal)

Optimization of 2′,4′-BNA/LNA-Based Oligonucleotides for Splicing Modulation In Vitro

Shimo, T., Obika, S.

Methods in Molecular Biology　Vol. 1828　p. 395-411　2018　Research paper (scientific journal)

Hydrogen peroxide-Triggered gene silencing in mammalian cells through boronated antisense oligonucleotides

Shohei Mori, Kunihiko Morihiro, Takumi Okuda, Yuuya Kasahara, Satoshi Obika

Chemical Science　Vol. 9　No. 5　p. 1112-1118　2018　Research paper (scientific journal)

Facile synthesis and fundamental properties of an N-methylguanidine-bridged nucleic acid (GuNA[NMe])

Naohiro Horie, Shinji Kumagai, Yutaro Kotobuki, Takao Yamaguchi, Satoshi Obika

Organic & Biomolecular Chemistry　2018　Research paper (scientific journal)

Development of oligonucleotide-based antagonists of Ebola virus protein 24 inhibiting its interaction with karyopherin alpha 1

Keisuke Tanaka, Yuuya Kasahara, Yoichi Miyamoto, Okuda Takumi, Tatsuro Kasai, Kentaro Onodera, Masayasu Kuwahara, Masahiro Oka, Yoshihiro Yoneda, Satoshi Obika

Organic and Biomolecular Chemistry　Vol. 16　No. 24　p. 4456-4463　2018　Research paper (scientific journal)

Correction: Development of oligonucleotide-based antagonists of Ebola virus protein 24 inhibiting its interaction with karyopherin alpha 1

Keisuke Tanaka, Yuuya Kasahara, Yoichi Miyamoto, Takumi Okuda, Tatsuro Kasai, Kentaro Onodera, Masayasu Kuwahara, Masahiro Oka, Yoshihiro Yoneda, Satoshi Obika

Organic & Biomolecular Chemistry　Vol. 16　No. 40　p. 7530-7530　2018　Research paper (scientific journal)

Antisense Oligonucleotides Targeting Y-Box Binding Protein-1 Inhibit Tumor Angiogenesis by Downregulating Bcl-xL-VEGFR2/-Tie Axes

Setoguchi, Kiyoko, Cui, Lin, Hachisuka, Nobutaka, Obchoei, Sumalee, Shinkai, Kentaro, Hyodo, Fuminori, Kato, Kiyoko, Wada, Fumito, Yamamoto, Tsuyoshi, Harada-Shiba, Mariko, Obika, Satoshi, Nakano, Kenji

MOLECULAR THERAPY-NUCLEIC ACIDS　Vol. 9　p. 170-181　2017/12　Research paper (scientific journal)

Synthesis and thermal stabilities of oligonucleotides containing 2 '-O,4 '-C-methylene bridged nucleic acid with a phenoxazine base

Yuki Kishimoto, Akane Fujii, Osamu Nakagawa, Tetsuya Nagata, Takanori Yokota, Yoshiyuki Hari, Satoshi Obika

ORGANIC & BIOMOLECULAR CHEMISTRY　Vol. 15　No. 38　p. 8145-8152　2017/10　Research paper (scientific journal)

Synthesis and thermal stabilities of oligonucleotides containing 2 '-O,4 '-C-methylene bridged nucleic acid with a phenoxazine base

Yuki Kishimoto, Akane Fujii, Osamu Nakagawa, Tetsuya Nagata, Takanori Yokota, Yoshiyuki Hari, Satoshi Obika

ORGANIC & BIOMOLECULAR CHEMISTRY　Vol. 15　No. 38　p. 8145-8152　2017/10　Research paper (scientific journal)

Small Nucleolar Noncoding RNA SNORA23, Up-Regulated in Human Pancreatic Ductal Adenocarcinoma, Regulates Expression of Spectrin Repeat-Containing Nuclear Envelope 2 to Promote Growth and Metastasis of Xenograft Tumors in Mice

Lin Cui, Kenji Nakano, Sumalee Obchoei, Kiyoko Setoguchi, Masaki Matsumoto, Tsuyoshi Yamamoto, Satoshi Obika, Kazuaki Shimada, Nobuyoshi Hiraoka

GASTROENTEROLOGY　Vol. 153　No. 1　p. 292-+　2017/07　Research paper (scientific journal)

Antisense oligo nucleotide of Annexin A4 improved platinum resistance in ovarian clear cell cancer

Kakubari Reisa, Nakagawa Satoshi, Iwamiya Tadashi, Kobayashi Eiji, Matsuzaki Shinnya, Ueda Yutaka, Yoshino Kiyoshi, Kasahara Yuya, Obika Satoshi, Kimura Tadashi, Serada Satoshi, Naka Tetsuji, Fujimoto Minoru

CANCER RESEARCH　Vol. 77　2017/07

Phospholamban Inhibition by a Single Dose of Locked Nucleic Acid Antisense Oligonucleotide Improves Cardiac Contractility in Pressure Overload-Induced Systolic Dysfunction in Mice

Hirofumi Morihara, Tsuyoshi Yamamoto, Harunori Oiwa, Kota Tonegawa, Daisuke Tsuchiyama, Ikki Kawakatsu, Masanori Obana, Makiko Maeda, Tomomi Mohri, Satoshi Obika, Yasushi Fujio, Hiroyuki Nakayama

JOURNAL OF CARDIOVASCULAR PHARMACOLOGY AND THERAPEUTICS　Vol. 22　No. 3　p. 273-282　2017/05　Research paper (scientific journal)

Synthesis, duplex-forming ability, enzymatic stability, and in vitro antisense potency of oligonucleotides including 2'-C,4'-C-ethyleneoxy-bridged thymidine derivatives

Takashi Osawa, Motoki Sawamura, Fumito Wada, Tsuyoshi Yamamoto, Satoshi Obika, Yoshiyuki Hari

ORGANIC & BIOMOLECULAR CHEMISTRY　Vol. 15　No. 18　p. 3955-3963　2017/05　Research paper (scientific journal)

Evaluation of size-exclusion chromatography for the analysis of phosphorothioate oligonucleotides

Atsuko Shimoyama, Aid Fujisaka, Satoshi Obika

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS　Vol. 136　p. 55-65　2017/03　Research paper (scientific journal)

Biological applications of xeno nucleic acids

Kunihiko Morihiro, Yuuya Kasahara, Satoshi Obika

MOLECULAR BIOSYSTEMS　Vol. 13　No. 2　p. 235-245　2017/02

Current Status of Nucleic Acid Drugs and the Challenges in Regulatory Science Relating to Quality Control

FUJISAKA Aki, RAMON ITO Kosuke, OBIKA Satoshi

Regulatory Science of Medical Products　Vol. 7　No. 2　p. 113-120　2017

Synthesis and Properties of 7-Deazapurine- and 8-Aza-7deazapurine-Locked Nucleic Acid Analogues: Effect of the Glycosidic Torsion Angle

Takashi Hara, Tetsuya Kodama, Yumi Takegaki, Kunihiko Morihiro, Kosuke Ramon Ito, Satoshi Obika

JOURNAL OF ORGANIC CHEMISTRY　Vol. 82　No. 1　p. 25-36　2017/01　Research paper (scientific journal)

SYNTHESIS OF THE 5-METHYLURIDINE MONOMER OF 3 '-O,4 '-C-ETHYLENEOXY-BRIDGED NUCLEIC ACID

Takashi Osawa, Masakazu Dohi, Yuka Hitomi, Yuta Ito, Satoshi Obika, Yoshiyuki Hari

HETEROCYCLES　Vol. 95　No. 1　p. 342-352　2017/01　Research paper (scientific journal)

Triazole- and Tetrazole-Bridged Nucleic Acids: Synthesis, Duplex Stability, Nuclease Resistance, and in Vitro and in Vivo Antisense Potency

Yasunori Mitsuoka, Tsuyoshi Yamamoto, Akira Kugimiya, Reiko Waki, Fumito Wada, Saori Tahara, Motoki Sawamura, Mio Noda, Yuko Fujimura, Yuki Kato, Yoshiyuki Hari, Satoshi Obika

JOURNAL OF ORGANIC CHEMISTRY　Vol. 82　No. 1　p. 12-24　2017/01　Research paper (scientific journal)

Synthesis and Properties of 7-Deazapurine- and 8-Aza-7deazapurine-Locked Nucleic Acid Analogues: Effect of the Glycosidic Torsion Angle

Takashi Hara, Tetsuya Kodama, Yumi Takegaki, Kunihiko Morihiro, Kosuke Ramon Ito, Satoshi Obika

JOURNAL OF ORGANIC CHEMISTRY　Vol. 82　No. 1　p. 25-36　2017/01　Research paper (scientific journal)

Systematic Evaluation of Biophysical and Functional Characteristics of Selenomethylene-Locked Nucleic Acid-Mediated Inhibition of miR-21

Smita Nahar, Amrita Singh, Kunihiko Morihiro, Yoshihiro Moai, Tetsuya Kodama, Satoshi Obika, Souvik Maiti

BIOCHEMISTRY　Vol. 55　No. 50　p. 7023-7032　2016/12　Research paper (scientific journal)

Synthesis of scpBNA-C-m, -A, and -G Monomers and Evaluation of the Binding Affinities of scpBNA-Modified Oligonucleotides toward Complementary ssRNA and ssDNA

Masahiko Horiba, Takao Yamaguchi, Satoshi Obika

JOURNAL OF ORGANIC CHEMISTRY　Vol. 81　No. 22　p. 11000-11008　2016/11　Research paper (scientific journal)

オリゴヌクレオチドを有効成分とする医薬品の品質管理に関する考え方

伊藤 浩介, 小林 直之, 橘 敬祐, 辻野 博文, 藤坂 朱紀, 小比賀 聡

日本核酸医薬学会誌　Vol. 20　No. 1　p. 15-23　2016/10

Synthesis and properties of novel 2 '-C,4 '-C-ethyleneoxy-bridged 2 '-deoxyribonucleic acids with exocyclic methylene groups

Takashi Osawa, Satoshi Obika, Yoshiyuki Hari

ORGANIC & BIOMOLECULAR CHEMISTRY　Vol. 14　No. 40　p. 9481-9484　2016/10　Research paper (scientific journal)

In vitro and in vivo biophysical properties of oligonucleotides containing 5 '-thio nucleosides

Islam Md Ariful, Waki Reiko, Fujisaka Aki, Ito Kosuke Ramon, Obika Satoshi

DRUG DISCOVERIES AND THERAPEUTICS　Vol. 10　No. 5　p. 263-270　2016/10

Design of antisense oligonucleotides.

Obika S, Kasahara Y

Nihon yakurigaku zasshi. Folia pharmacologica Japonica　Vol. 148　No. 2　p. 100-104　2016/08　Research paper (scientific journal)

Nuclear expression of Y-box binding protein-1 is associated with poor prognosis in patients with pancreatic cancer and its knockdown inhibits tumor growth and metastasis in mice tumor models

Kentaro Shinkai, Kenji Nakano, Lin Cui, Yusuke Mizuuchi, Hideya Onishi, Yoshinao Oda, Satoshi Obika, Masao Tanaka, Mitsuo Katano

INTERNATIONAL JOURNAL OF CANCER　Vol. 139　No. 2　p. 433-445　2016/07　Research paper (scientific journal)

Synthesis and properties of 4-(diarylmethylene)imidazolinone-conjugated fluorescent nucleic acids

Takumi Okuda, Shohei Mori, Yuuya Kasahara, Kunihiko Morihiro, Masahiro Ikejiri, Kazuyuki Miyashita, Satoshi Obika

TETRAHEDRON LETTERS　Vol. 57　No. 29　p. 3129-3132　2016/07　Research paper (scientific journal)

Evaluation of the effects of chemically different linkers on hepatic accumulations, cell tropism and gene silencing ability of cholesterol-conjugated antisense oligonucleotides

Shunsuke Wada, Hidenori Yasuhara, Fumito Wada, Motoki Sawamura, Reiko Waki, Tsuyoshi Yamamoto, Mariko Harada-Shiba, Satoshi Obika

JOURNAL OF CONTROLLED RELEASE　Vol. 226　p. 57-65　2016/03　Research paper (scientific journal)

Synthesis and Properties of 2 ',4 '-Bridged Nucleic Acids Containing Multiple Heteroatoms in the Bridges

Yoshiyuki Hari, Satoshi Obika

JOURNAL OF SYNTHETIC ORGANIC CHEMISTRY JAPAN　Vol. 74　No. 2　p. 141-153　2016/02

Analysis of hybridization-dependent off-target effects of antisense oligonucleotides

YOSHIDA Tokuyuki, NAITO Yuki, SASAKI Kiyomi, UCHIDA Eriko, OBIKA Satoshi, NAITO Mikihiko, INOUE Takao

Annual Meeting of the Japanese Society of Toxicology　Vol. 43　p. P-94　2016

Serial incorporation of a monovalent GalNAc phosphoramidite unit into hepatocyte-targeting antisense oligonucleotides

Tsuyoshi Yamamoto, Motoki Sawamura, Fumito Wada, Mariko Harada-Shiba, Satoshi Obika

BIOORGANIC & MEDICINAL CHEMISTRY　Vol. 24　No. 1　p. 26-32　2016/01　Research paper (scientific journal)

Consecutive incorporation of functionalized nucleotides with amphiphilic side chains by novel KOD polymerase mutant

Hidekazu Hoshino, Yuuya Kasahara, Hiroto Fujita, Masayasu Kuwahara, Kunihiko Morihiro, Shin-ichi Tsunoda, Satoshi Obika

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS　Vol. 26　No. 2　p. 530-533　2016/01　Research paper (scientific journal)

The crystal structure of a 2 ',4 '-BNA(NC)[N-Me]-modified antisense gapmer in complex with the target RNA

Jiro Kondo, Yusuke Nomura, Yukiko Kitahara, Satoshi Obika, Hidetaka Torigoe

CHEMICAL COMMUNICATIONS　Vol. 52　No. 11　p. 2354-2357　2016　Research paper (scientific journal)

Effect of an N-substituent in sulfonamide-bridged nucleic acid (SuNA) on hybridization ability and duplex structure

Yasunori Mitsuoka, Hiroshi Aoyama, Akira Kugimiya, Yuko Fujimura, Tsuyoshi Yamamoto, Reiko Waki, Fumito Wada, Saori Tahara, Motoki Sawamura, Mio Noda, Yoshiyuki Hari, Satoshi Obika

ORGANIC & BIOMOLECULAR CHEMISTRY　Vol. 14　No. 27　p. 6531-6538　2016　Research paper (scientific journal)

Hetero-chimera-duplex-oligonucleotideによるエクソンスキッピング療法の開発(Development of exon-skipping therapy by hetero-chimera-duplex oligonucleotide)

吉岡 耕太郎, 筋野 裕美子, 國枝 泰希, 下 剛典, 田中 規恵, 朴 文英, 桑原 宏哉, 仁科 一隆, 小比賀 聡, 横田 隆徳

臨床神経学　Vol. 55　No. Suppl.　p. S212-S212　2015/12

Synthesis and Properties of the 5-Methyluridine Derivative of 3,4-Dihydro-2H-pyran-Bridged Nucleic Acid (DpNA)

Takashi Osawa, Yoshiyuki Hari, Masakazu Dohi, Yuya Matsuda, Satoshi Obika

JOURNAL OF ORGANIC CHEMISTRY　Vol. 80　No. 21　p. 10474-10481　2015/11　Research paper (scientific journal)

Ca2+ enrichment in culture medium potentiates effect of oligonucleotides

Shin-ichiro Hori, Tsuyoshi Yamamoto, Reiko Waki, Shunsuke Wada, Fumito Wada, Mio Noda, Satoshi Obika

NUCLEIC ACIDS RESEARCH　Vol. 43　No. 19　2015/10　Research paper (scientific journal)

Polymerase incorporation of a 2 '-deoxynucleoside-5 '-triphosphate bearing a 4-hydroxy-2-mercaptobenzimidazole nucleobase analogue

Kunihiko Morihiro, Hidekazu Hoshino, Osamu Hasegawa, Yuuya Kasahara, Kohsuke Nakajima, Masayasu Kuwahara, Shin-ichi Tsunoda, Satoshi Obika

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS　Vol. 25　No. 15　p. 2888-2891　2015/08　Research paper (scientific journal)

XRN2 is required for the degradation of target RNAs by RNase H1-dependent antisense oligonucleotides

Shin-Ichiro Hori, Tsuyoshi Yamamoto, Satoshi Obika

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　Vol. 464　No. 2　p. 506-511　2015/08　Research paper (scientific journal)

DNA/RNA heteroduplex oligonucleotide for highly efficient gene silencing

Kazutaka Nishina, Wenying Piao, Kie Yoshida-Tanaka, Yumiko Sujino, Tomoko Nishina, Tsuyoshi Yamamoto, Keiko Nitta, Kotaro Yoshioka, Hiroya Kuwahara, Hidenori Yasuhara, Takeshi Baba, Fumiko Ono, Kanjiro Miyata, Koichi Miyake, Punit P. Seth, Audrey Low, Masayuki Yoshida, C. Frank Bennett, Kazunori Kataoka, Hidehiro Mizusawa, Satoshi Obika, Takanori Yokota

NATURE COMMUNICATIONS　Vol. 6　2015/08　Research paper (scientific journal)

Synthesis and fluorescence properties of 4-diarylmethylene analogues of the green fluorescent protein chromophore

Masahiro Ikejiri, Kousuke Matsumoto, Hiraku Hasegawa, Daisuke Yamaguchi, Moe Tsuchino, Yoshiko Chihara, Takao Yamaguchi, Kazuto Mori, Takeshi Imanishi, Satoshi Obika, Kazuyuki Miyashita

TETRAHEDRON　Vol. 71　No. 30　p. 4987-4998　2015/07　Research paper (scientific journal)

Synthesis and properties of 2'-deoxyuridine analogues bearing various azobenzene derivatives at the C5 position

Mori, S., Morihiro, K., Kasahara, Y., Tsunoda, S.-I., Obika, S.

Chemosensors　Vol. 3　No. 2　p. 36-54　2015/06　Research paper (scientific journal)

C5-azobenzene-functionalized locked nucleic acid uridine: Isomerization properties, hybridization ability, and enzymatic stability

Morihiro, K., Hasegawa, O., Mori, S., Tsunoda, S., Obika, S.

Organic and Biomolecular Chemistry　Vol. 13　No. 18　p. 5209-5214　2015/05　Research paper (scientific journal)

In vitro selection of DNA-based aptamers that exhibit RNA-like conformations using a chimeric oligonucleotide library that contains two different xeno-nucleic acids

Kenta Hagiwara, Hiroto Fujita, Yuuya Kasahara, Yuuta Irisawa, Satoshi Obika, Masayasu Kuwahara

MOLECULAR BIOSYSTEMS　Vol. 11　No. 1　p. 71-76　2015/01　Research paper (scientific journal)

Synthesis and hybridization property of a boat-shaped pyranosyl nucleic acid containing an exocyclic methylene group in the sugar moiety

Kazuto Mori, Tetsuya Kodama, Satoshi Obika

BIOORGANIC & MEDICINAL CHEMISTRY　Vol. 23　No. 1　p. 33-37　2015/01　Research paper (scientific journal)

BASE PAIR RECOGNITION ABILITY OF 2-(METHYLAMINO)PYRIMIDIN-4-YL NUCLEOBASE IN PARALLEL TRIPLEX DNA

Yoshiyuld Hari, Satoshi Kashima, Yuya Matsuda, Akihiro Sakata, Ryutaro Takamine, Shin Ijitsu, Satoshi Obika

HETEROCYCLES　Vol. 90　No. 1　p. 432-441　2015/01　Research paper (scientific journal)

Amido-bridged nucleic acids with small hydrophobic residues enhance hepatic tropism of antisense oligonucleotides in vivo

Tsuyoshi Yamamoto, Aiko Yahara, Reiko Waki, Hidenori Yasuhara, Fumito Wada, Mariko Harada-Shiba, Satoshi Obika

ORGANIC & BIOMOLECULAR CHEMISTRY　Vol. 13　No. 12　p. 3757-3765　2015　Research paper (scientific journal)

MicroRNAs Induce Epigenetic Reprogramming and Suppress Malignant Phenotypes of Human Colon Cancer Cells.

Hisataka Ogawa, Xin Wu, Koichi Kawamoto, Naohiro Nishida, Masamitsu Konno, Jun Koseki, Hidetoshi Matsui, Kozou Noguchi, Noriko Gotoh, Tsuyoshi Yamamoto, Kanjiro Miyata, Nobuhiro Nishiyama, Hiroaki Nagano, Hirofumi Yamamoto, Satoshi Obika, Kazunori Kataoka, Yuichiro Doki, Masaki Mori, Hideshi Ishii

PloS one　Vol. 10　No. 5　2015　Research paper (scientific journal)

Synthesis and properties of 2 '-O,4 '-C-spirocyclo-propylene bridged nucleic acid (scpBNA), an analogue of 2 ',4 '-BNA/LNA bearing a cyclopropane ring

Takao Yamaguchi, Masahiko Horiba, Satoshi Obika

CHEMICAL COMMUNICATIONS　Vol. 51　No. 47　p. 9737-9740　2015　Research paper (scientific journal)

Nucleic acid delivery systems based on poly(galactosyl ureaethyl methacrylate-b-dimethylamino ethyl methacrylate) diblock copolymers

M. C. Munisso, S. Obika, T. Yamaoka

Carbohydrate Polymers　Vol. 114　p. 288-296　2014/12/19　Research paper (scientific journal)

Sulfonamide-Bridged Nucleic Acid: Synthesis, High RNA Selective Hybridization, and High Nuclease Resistance

Yasunori Mitsuoka, Yuko Fujimura, Reiko Waki, Akira Kugimiya, Tsuyoshi Yamamoto, Yoshiyuki Hari, Satoshi Obika

ORGANIC LETTERS　Vol. 16　No. 21　p. 5640-5643　2014/11　Research paper (scientific journal)

Evaluation of Multiple-Turnover Capability of Locked Nucleic Acid Antisense Oligonucleotides in Cell-Free RNase H-Mediated Antisense Reaction and in Mice

Tsuyoshi Yamamoto, Naoko Fujii, Hidenori Yasuhara, Shunsuke Wada, Fumito Wada, Naoya Shigesada, Mariko Harada-Shiba, Satoshi Obika

NUCLEIC ACID THERAPEUTICS　Vol. 24　No. 4　p. 283-290　2014/08　Research paper (scientific journal)

Kinetic study of the binding of triplex-forming oligonucleotides containing partial cationic modifications to double-stranded DNA

Yoshiyuki Hari, Shin Ijitsu, Masaaki Akabane-Nakata, Takuya Yoshida, Satoshi Obika

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS　Vol. 24　No. 14　p. 3046-3049　2014/07　Research paper (scientific journal)

Development of External Stimuli-Responsive Nucleic Acids by Sugar, Backbone, and Nucleobase Modification

Kunihiko Morihiro, Tetsuya Kodama, Satoshi Obika

SYNLETT　Vol. 25　No. 11　p. 1499-1510　2014/07　Research paper (scientific journal)

Target Gene Knockdown by 2 ',4 '-BNA/LNA Antisense Oligonucleotides in Zebrafish

Motoyuki Itoh, Mizuki Nakaura, Takeshi Imanishi, Satoshi Obika

NUCLEIC ACID THERAPEUTICS　Vol. 24　No. 3　p. 186-191　2014/06　Research paper (scientific journal)

Quantitative evaluation of the improvement in the pharmacokinetics of a nucleic acid drug delivery system by dynamic PET imaging with F-18-incorporated oligodeoxynucleotides

Hidefumi Mukai, Daiki Ozaki, Yilong Cui, Takeshi Kuboyama, Hiroko Yamato-Nagata, Kayo Onoe, Maiko Takahashi, Yasuhiro Wada, Takeshi Imanishi, Tetsuya Kodama, Satoshi Obika, Masaaki Suzuki, Hisashi Doi, Yasuyoshi Watanabe

JOURNAL OF CONTROLLED RELEASE　Vol. 180　p. 92-99　2014/04　Research paper (scientific journal)

C5-Azobenzene- substituted 2 '-Deoxyuridine-containing Oligodeoxynucleotides for Photo-Switching Hybridization

Shohei Mori, Kunihiko Morihiro, Satoshi Obika

MOLECULES　Vol. 19　No. 4　p. 5109-5118　2014/04　Research paper (scientific journal)

Synthesis and characterization of benzylidene acetal-type bridged nucleic acids (BA-BNA)

Tetsuya Kodama, Kunihiko Morihiro, Satoshi Obika

Current Protocols in Nucleic Acid Chemistry　Vol. 2014　p. 1.31.1-1.31.22　2014　Research paper (scientific journal)

Guanidine bridged nucleic acid (GuNA): an effect of a cationic bridged nucleic acid on DNA binding affinity

Ajaya R. Shrestha, Yutaro Kotobuki, Yoshiyuki Hari, Satoshi Obika

CHEMICAL COMMUNICATIONS　Vol. 50　No. 5　p. 575-577　2014　Research paper (scientific journal)

Light-triggered strand exchange reaction using the change in the hydrogen bonding pattern of a nucleobase analogue

Kunihiko Morihiro, Tetsuya Kodama, Reiko Waki, Satoshi Obika

CHEMICAL SCIENCE　Vol. 5　No. 2　p. 744-750　2014　Research paper (scientific journal)

THE ABILITY OF 1-ARYLTRIAZOLE-CONTAINING NUCLEOBASES TO RECOGNIZE A TA BASE PAIR IN TRIPLEX DNA

Yoshiyuki Hari, Motoi Nakahara, Shin Ijitsu, Satoshi Obika

HETEROCYCLES　Vol. 88　No. 1　p. 377-386　2014/01　Research paper (scientific journal)

Locked nucleic acid antisense inhibitor targeting apolipoprotein C-III efficiently and preferentially removes triglyceride from large very low-density lipoprotein particles in murine plasma

Tsuyoshi Yamamoto, Satoshi Obika, Moeka Nakatani, Hidenori Yasuhara, Fumito Wada, Eiko Shibata, Masa-Aki Shibata, Mariko Harada-Shiba

EUROPEAN JOURNAL OF PHARMACOLOGY　Vol. 723　p. 353-359　2014/01　Research paper (scientific journal)

Photoinduced changes in hydrogen bonding patterns of 8-thiopurine nucleobase analogues in a DNA strand

Kunihiko Morihiro, Tetsuya Kodama, Shohei Mori, Satoshi Obika

ORGANIC & BIOMOLECULAR CHEMISTRY　Vol. 12　No. 15　p. 2468-2473　2014　Research paper (scientific journal)

Design and evaluation of locked nucleic acid-based splice-switching oligonucleotides in vitro

Takenori Shimo, Keisuke Tachibana, Kiwamu Saito, Tokuyuki Yoshida, Erisa Tomita, Reiko Waki, Tsuyoshi Yamamoto, Takefumi Doi, Takao Inoue, Junji Kawakami, Satoshi Obika

NUCLEIC ACIDS RESEARCH　Vol. 42　No. 12　p. 8174-8187　2014　Research paper (scientific journal)

Synthesis of oligonucleotides containing N,N-disubstituted 3-deazacytosine nucleobases by post-elongation modification and their triplex-forming ability with double-stranded DNA

Masaaki Akabane-Nakata, Satoshi Obika, Yoshiyuki Hari

ORGANIC & BIOMOLECULAR CHEMISTRY　Vol. 12　No. 44　p. 9011-9015　2014　Research paper (scientific journal)

Triplex-forming ability of oligonucleotides containing 1-aryl-1,2,3- triazole nucleobases linked via a two atom-length spacer

Yoshiyuki Hari, Motoi Nakahara, Satoshi Obika

Bioorganic and Medicinal Chemistry　Vol. 21　No. 17　p. 5583-5588　2013/09/01　Research paper (scientific journal)

Synthesis of novel polyesteramine dendrimers by divergent and convergent methods

Hitoshi Akiyama, Kazuyuki Miyashita, Yoshiyuki Hari, Satoshi Obika, Takeshi Imanishi

Tetrahedron　Vol. 69　No. 33　p. 6810-6820　2013/08/19　Research paper (scientific journal)

Base-pair recognition ability of hydroxyphenyl nucleobases in parallel triplex DNA

Yoshiyuki Hari, Satoshi Kashima, Hiroyasu Inohara, Shin Ijitsu, Takeshi Imanishi, Satoshi Obika

Tetrahedron　Vol. 69　No. 31　p. 6381-6391　2013/08/05　Research paper (scientific journal)

Synthesis and properties of thymidines with six-membered amide bridge

Yoshiyuki Hari, Takashi Osawa, Yutaro Kotobuki, Aiko Yahara, Ajaya R. Shrestha, Satoshi Obika

Bioorganic and Medicinal Chemistry　Vol. 21　No. 14　p. 4405-4412　2013/07/15　Research paper (scientific journal)

Synthesis and Properties of 2 '-O,4 '-C-Ethyleneoxy Bridged 5-Methyluridine

Yoshiyuki Hari, Tomohiko Morikawa, Takashi Osawa, Satoshi Obika

ORGANIC LETTERS　Vol. 15　No. 14　p. 3702-3705　2013/07　Research paper (scientific journal)

Capillary Electrophoresis-Systematic Evolution of Ligands by Exponential Enrichment Selection of Base- and Sugar-Modified DNA Aptamers: Target Binding Dominated by 2 '-O,4 '-C-Methylene-Bridged/Locked Nucleic Acid Primer

Yuuya Kasahara, Yuuta Irisawa, Hiroto Fujita, Aiko Yahara, Hiroaki Ozaki, Satoshi Obika, Masayasu Kuwahara

ANALYTICAL CHEMISTRY　Vol. 85　No. 10　p. 4961-4967　2013/05　Research paper (scientific journal)

2 ',4 '-BNA/LNA aptamers: CE-SELEX using a DNA-based library of full-length 2 '-O,4 '-C-methylene-bridged/linked bicyclic ribonucleotides

Yuuya Kasahara, Yuuta Irisawa, Hiroaki Ozaki, Satoshi Obika, Masayasu Kuwahara

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS　Vol. 23　No. 5　p. 1288-1292　2013/03　Research paper (scientific journal)

In vitro selection of BNA (LNA) aptamers

Masayasu Kuwahara, Satoshi Obika

Artificial DNA: PNA and XNA　Vol. 4　No. 2　p. 39-48　2013　Research paper (scientific journal)

Selenomethylene Locked Nucleic Acid Enables Reversible Hybridization in Response to Redox Changes

Kunihiko Morihiro, Tetsuya Kodama, Kentefu, Yoshihiro Moai, Rakesh N. Veedu, Satoshi Obika

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION　Vol. 52　No. 19　p. 5074-5078　2013　Research paper (scientific journal)

2 ',4 '-BNA bearing a chiral guanidinopyrrolidine-containing nucleobase with potent ability to recognize the CG base pair in a parallel-motif DNA triplex

Yoshiyuki Hari, Masaaki Akabane, Satoshi Obika

CHEMICAL COMMUNICATIONS　Vol. 49　No. 67　p. 7421-7423　2013　Research paper (scientific journal)

A 2-AMINO-6-METHYLPYRIDIN-5-YL NUCLEOBASE FOR GC BASE PAIR RECOGNITION IN THE PARALLEL TRIPLEX DNA

Motoi Nakahara, Yoshiyuki Hari, Satoshi Obika

HETEROCYCLES　Vol. 86　No. 2　p. 1135-1146　2012/12　Research paper (scientific journal)

Amido-Bridged Nucleic Acids (AmNAs): Synthesis, Duplex Stability, Nuclease Resistance, and in Vitro Antisense Potency

Aiko Yahara, Ajaya Ram Shrestha, Tsuyoshi Yamamoto, Yoshiyuki Hari, Takashi Osawa, Masaki Yamaguchi, Masaru Nishida, Tetsuya Kodama, Satoshi Obika

CHEMBIOCHEM　Vol. 13　No. 17　p. 2513-2516　2012/11　Research paper (scientific journal)

Efficient reduction of serum cholesterol by combining a liver-targeted gene delivery system with chemically modified apolipoprotein B siRNA

Jeong-Hun Kang, Yoichi Tachibana, Satoshi Obika, Mariko Harada-Shiba, Tetsuji Yamaoka

JOURNAL OF CONTROLLED RELEASE　Vol. 163　No. 2　p. 119-124　2012/10　Research paper (scientific journal)

Design and Concise Synthesis of a Novel Type of Green Fluorescent Protein Chromophore Analogue

Masahiro Ikejiri, Moe Tsuchino, Yoshiko Chihara, Takao Yamaguchi, Takeshi Imanishi, Satoshi Obika, Kazuyuki Miyashita

ORGANIC LETTERS　Vol. 14　No. 17　p. 4406-4409　2012/09　Research paper (scientific journal)

Development of a 2 ',4 '-BNA/LNA-based siRNA for Dyslipidemia and Assessment of the Effects of Its Chemical Modifications In Vivo

Shunsuke Wada, Satoshi Obika, Masa-Aki Shibata, Tsuyoshi Yamamoto, Moeka Nakatani, Tetsuji Yamaoka, Hidetaka Torigoe, Mariko Harada-Shiba

MOLECULAR THERAPY-NUCLEIC ACIDS　Vol. 1　2012/09　Research paper (scientific journal)

Hybridizing ability and nuclease resistance profile of backbone modified cationic phosphorothioate oligonucleotides

S. M. Abdur Rahman, Takeshi Baba, Tetsuya Kodama, Md Ariful Islam, Satoshi Obika

BIOORGANIC & MEDICINAL CHEMISTRY　Vol. 20　No. 13　p. 4098-4102　2012/07　Research paper (scientific journal)

Effect of Continuous Exposure to Antisense BNA Slowly Released in Intraperitoneal or Subcutaneous Tissue

Tetsuji Yamaoka, Mariko Shiba, Hidetaka Torigoe, Satoshi Obika, Atsushi Yamashita, Yoichi Tachibana

MOLECULAR THERAPY　Vol. 20　p. S105-S105　2012/05

Towards the Sequence-Selective Recognition of Double-Stranded DNA Containing Pyrimidine-Purine Interruptions by Triplex-Forming Oligonucleotides

Yoshiyuki Hari, Satoshi Obika, Takeshi Imanishi

EUROPEAN JOURNAL OF ORGANIC CHEMISTRY　No. 15　p. 2875-2887　2012/05

Cholesterol-lowering Action of BNA-based Antisense Oligonucleotides Targeting PCSK9 in Atherogen Diet-induced Hypercholesterolemic Mice

Tsuyoshi Yamamoto, Mariko Harada-Shiba, Moeka Nakatani, Shunsuke Wada, Hidenori Yasuhara, Keisuke Narukawa, Kiyomi Sasaki, Masa-Aki Shibata, Hidetaka Torigoe, Tetsuji Yamaoka, Takeshi Imanishi, Satoshi Obika

MOLECULAR THERAPY-NUCLEIC ACIDS　Vol. 1　2012/05　Research paper (scientific journal)

Chemical modification of triplex-forming oligonucleotide to promote pyrimidine motif triplex formation at physiological pH

Hidetaka Torigoe, Osamu Nakagawa, Takeshi Imanishi, Satoshi Obika, Kiyomi Sasaki

BIOCHIMIE　Vol. 94　No. 4　p. 1032-1040　2012/04　Research paper (scientific journal)

網膜の包括的神経保護 臨床応用への挑戦

石橋 達朗, 吉田 茂生, 江内田 寛, 池田 康博, 久冨 智朗, 武田 篤信, 中尾 新太郎, 大島 裕司, 宮崎 勝徳, 村上 祐介, 有田 量一, 川原 周平, 石川 桂二郎, 吉田 倫子, 納富 昭司, Miller Joan W., 出原 賢治, 小比賀 聡, 門田 幸二, 向野 利寛, 園田 康平, 蜂須賀 祥行, 藤田 克実, 小林 俊洋, 上野 登輝夫, 小林 正彦, 上田 泰次, 長谷川 護, 小野 純也, 鍵本 忠尚, 居石 克夫, 赤司 浩一, 米満 吉和, 吉永 幸靖, 中村 大輔, 岡田 龍雄, 中別府 雄作, 中村 崇規, 池尾 一穂, 五條堀 孝

日本眼科学会雑誌　Vol. 116　No. 3　p. 165-199　2012/03

Superior Silencing by 2',4'-BNA(NC)-Based Short Antisense Oligonucleotides Compared to 2',4'-BNA/LNA-Based Apolipoprotein B Antisense Inhibitors.

Tsuyoshi Yamamoto, Hidenori Yasuhara, Fumito Wada, Mariko Harada-Shiba, Takeshi Imanishi, Satoshi Obika

Journal of nucleic acids　Vol. 2012　p. 707323-707323　2012　Research paper (scientific journal)

Synthesis and evaluation of novel caged DNA alkylating agents bearing 3,4-epoxypiperidine structure

Yuji Kawada, Tetsuya Kodama, Kazuyuki Miyashita, Takeshi Imanishi, Satoshi Obika

ORGANIC & BIOMOLECULAR CHEMISTRY　Vol. 10　No. 26　p. 5102-5108　2012　Research paper (scientific journal)

Synthesis of selenomethylene-locked nucleic acid (SeLNA)-modified oligonucleotides by polymerases

Megan Wheeler, Antoine Chardon, Astrid Goubet, Kunihiko Morihiro, Sze Yee Tsan, Stacey L. Edwards, Tetsuya Kodama, Satoshi Obika, Rakesh N. Veedu

CHEMICAL COMMUNICATIONS　Vol. 48　No. 89　p. 11020-11022　2012　Research paper (scientific journal)

Synthesis and duplex-forming ability of oligonucleotides containing 4 '-carboxythymidine

Yoshiyuki Hari, Takashi Osawa, Satoshi Obika

ORGANIC & BIOMOLECULAR CHEMISTRY　Vol. 10　No. 48　p. 9639-9649　2012　Research paper (scientific journal)

Synthesis and Properties of a Bridged Nucleic Acid with a Perhydro-1,2-oxazin-3-one Ring

Ajaya R. Shrestha, Yoshiyuki Hari, Aiko Yahara, Takashi Osawa, Satoshi Obika

JOURNAL OF ORGANIC CHEMISTRY　Vol. 76　No. 24　p. 9891-9899　2011/12　Research paper (scientific journal)

Cleavage of Oligonucleotides Containing a P3' -> N5' Phosphoramidate Linkage Mediated by Single-Stranded Oligonucleotide Templates

Kosuke Ramon Ito, Tetsuya Kodama, Futaba Makimura, Noritsugu Hosoki, Tomohisa Osaki, Ayako Orita, Takeshi Imanishi, Satoshi Obika

MOLECULES　Vol. 16　No. 12　p. 10695-10708　2011/12　Research paper (scientific journal)

Design, Synthesis, and Properties of Boat-Shaped Glucopyranosyl Nucleic Acid

Kazuto Mori, Tetsuya Kodama, Satoshi Obika

ORGANIC LETTERS　Vol. 13　No. 22　p. 6050-6053　2011/11　Research paper (scientific journal)

POTENCY COMPARISON OF SERIES OF BNA-BASED ANTISENSES IN DYSLIPIDEMIC ANIMALS

Tsuyoshi Yamamoto, Mariko Harada-Shiba, Moeka Nakatani, Hidenori Yasuhara, Shunsuke Wada, Keisuke Narukawa, Kiyomi Sasaki, Hidetaka Torigoe, Tetsuji Yamaoka, Takeshi Imanishi, Satoshi Obika

NUCLEIC ACID THERAPEUTICS　Vol. 21　No. 5　p. A30-A31　2011/10

Nucleotide modification and polymerase engineering for creating a novel class of artificial nucleic acid aptamers.

Imaizumi Y, Takano Y, Kitadume S, Ozaki H, Obika S, Kuwahara M

IFMBE Proceedings　Vol. 37　p. 1023-1026　2011/09/14　Research paper (international conference proceedings)

Benzylidene Acetal Type Bridged Nucleic Acids: Changes in Properties Upon Cleavage of the Bridge Triggered by External Stimuli

Kunihiko Morihiro, Tetsuya Kodama, Satoshi Obika

CHEMISTRY-A EUROPEAN JOURNAL　Vol. 17　No. 28　p. 7918-7926　2011/07　Research paper (scientific journal)

Increased Expression of Periostin in Vitreous and Fibrovascular Membranes Obtained from Patients with Proliferative Diabetic Retinopathy

Shigeo Yoshida, Keijiro Ishikawa, Ryo Asato, Mitsuru Arima, Yukio Sassa, Ayako Yoshida, Hiroshi Yoshikawa, Keisuke Narukawa, Satoshi Obika, Junya Ono, Shoichiro Ohta, Kenji Izuhara, Toshihiro Kono, Tatsuro Ishibashi

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE　Vol. 52　No. 8　p. 5670-5678　2011/07　Research paper (scientific journal)

PETを用いた核酸医薬DDSの動態評価 クリック化学法による18F-標識化オリゴヌクレオチドの有用性の実証

向井 英史, 尾崎 大起, 長田 浩子, 崔 翼龍, 窪山 剛之, 和田 康弘, 今西 武, 兒玉 哲也, 小比賀 聡, 鈴木 正昭, 土居 久志, 渡辺 恭良

Drug Delivery System　Vol. 26　No. 3　p. 294-294　2011/05

Antisense drug discovery and development

Tsuyoshi Yamamoto, Moeka Nakatani, Keisuke Narukawa, Satoshi Obika

FUTURE MEDICINAL CHEMISTRY　Vol. 3　No. 3　p. 339-365　2011/03

Antisense drug discovery and development

Tsuyoshi Yamamoto, Moeka Nakatani, Keisuke Narukawa, Satoshi Obika

FUTURE MEDICINAL CHEMISTRY　Vol. 3　No. 3　p. 339-365　2011/03

Synthesis and triplex-forming ability of oligonucleotides bearing 1-substituted 1H-1,2,3-triazole nucleobases

Yoshiyuki Hari, Motoi Nakahara, Juanjuan Pang, Masaaki Akabane, Takeshi Kuboyama, Satoshi Obika

BIOORGANIC & MEDICINAL CHEMISTRY　Vol. 19　No. 3　p. 1162-1166　2011/02　Research paper (scientific journal)

2 '-O,4 '-C-Aminomethylene-Bridged Nucleic Acid Modification with Enhancement of Nuclease Resistance Promotes Pyrimidine Motif Triplex Nucleic Acid Formation at Physiological pH

Hidetaka Torigoe, S. M. Abdur Rahman, Hiroko Takuma, Norihiro Sato, Takeshi Imanishi, Satoshi Obika, Kiyomi Sasaki

CHEMISTRY-A EUROPEAN JOURNAL　Vol. 17　No. 9　p. 2742-2751　2011/02　Research paper (scientific journal)

Design and facile synthesis of a novel 2’,4’-bridged nucleic acid

Ajaya R. Shrestha, Yoshiyuki Hari, Aiko Yahara, Takashi Osawa, Satoshi Obika

Collection Symposium Series, XVth Symposium on Chemistry of Nucleic Acid Components　Vol. 12　p. 144-147　2011　Research paper (conference, symposium, etc.)

2′-O,4′-C-Methyleneoxymethylene Bridged Nucleic Acids (2′,4′-BNA^COC)

Hari, Y., Kodama, T., Imanishi, T., Obika, S.

Methods in Molecular Biology　Vol. 764　p. 31-57　2011　Research paper (scientific journal)

Stoichiometry-focused F-18-labeling of alkyne-substituted oligodeoxynucleotides using azido([F-18]fluoromethyl)benzenes by Cu-catalyzed Huisgen reaction

Takeshi Kuboyama, Motoi Nakahara, Masafumi Yoshino, Yilong Cui, Takeo Sako, Yasuhiro Wada, Takeshi Imanishi, Satoshi Obika, Yasuyoshi Watanabe, Masaaki Suzuki, Hisashi Doi

BIOORGANIC & MEDICINAL CHEMISTRY　Vol. 19　No. 1　p. 249-255　2011/01　Research paper (scientific journal)

Interrupted 2 '-O,4 '-C-Aminomethylene Bridged Nucleic Acid Modification Enhances Pyrimidine Motif Triplex-Forming Ability and Nuclease Resistance Under Physiological Condition

Hidetaka Torigoe, S. M. Abdur Rahman, Hiroko Takuma, Norihiro Sato, Takeshi Imanishi, Satoshi Obika, Kiyomi Sasaki

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS　Vol. 30　No. 1　p. 63-81　2011　Research paper (scientific journal)

A 4-[(3R,4R)-dihydroxypyrrolidino]pyrimidin-2-one nucleobase for a CG base pair in triplex DNA

Yoshiyuki Hari, Masaaki Akabane, Yuri Hatanaka, Motoi Nakahara, Satoshi Obika

CHEMICAL COMMUNICATIONS　Vol. 47　No. 15　p. 4424-4426　2011　Research paper (scientific journal)

Bridged nucleic acid conjugates at 6 '-thiol: synthesis, hybridization properties and nuclease resistances

Kazuto Mori, Tetsuya Kodama, Takeshi Baba, Satoshi Obika

ORGANIC & BIOMOLECULAR CHEMISTRY　Vol. 9　No. 14　p. 5272-5279　2011　Research paper (scientific journal)

Double-stranded DNA-templated cleavage of oligonucleotides containing a P3'-> N5' linkage triggered by triplex formation: the effects of chemical modifications and remarkable enhancement in reactivity

Kosuke Ramon Ito, Tetsuya Kodama, Masaharu Tomizu, Yoshinori Negoro, Ayako Orita, Tomohisa Osaki, Noritsugu Hosoki, Takaya Tanaka, Takeshi Imanishi, Satoshi Obika

NUCLEIC ACIDS RESEARCH　Vol. 38　No. 20　p. 7332-7342　2010/11　Research paper (scientific journal)

Study on Suitability of KOD DNA Polymerase for Enzymatic Production of Artificial Nucleic Acids Using Base/Sugar Modified Nucleoside Triphosphates

Masayasu Kuwahara, Yuuki Takano, Yuuya Kasahara, Hiroki Nara, Hiroaki Ozaki, Hiroaki Sawai, Akio Sugiyama, Satoshi Obika

MOLECULES　Vol. 15　No. 11　p. 8229-8240　2010/11　Research paper (scientific journal)

Synthesis and DNA cleavage evaluation of epoxypiperidine derivatives bearing a dehydroamino acid unit

Takao Yamaguchi, Yuji Kawada, Satoshi Obika, Takeshi Imanishi, Kazuyuki Miyashita

TETRAHEDRON　Vol. 66　No. 41　p. 8181-8188　2010/10　Research paper (scientific journal)

BRIDGED NUCLEIC ACIDS: DEVELOPMENT, SYNTHESIS AND PROPERTIES

Satoshi Obika, S. M. Abdur Rahman, Aki Fujisaka, Yuji Kawada, Takeshi Baba, Takeshi Imanishi

HETEROCYCLES　Vol. 81　No. 6　p. 1347-1392　2010/06

RNA interference with 2 ',4 '-bridged nucleic acid analogues

S. M. Abdur Rahman, Hiroyuki Sato, Naoto Tsuda, Sunao Haitani, Keisuke Narukawa, Takeshi Imanishi, Satoshi Obika

BIOORGANIC & MEDICINAL CHEMISTRY　Vol. 18　No. 10　p. 3474-3480　2010/05　Research paper (scientific journal)

Effect of 3 '-end capping of aptamer with various 2 ',4 '-bridged nucleotides: Enzymatic post-modification toward a practical use of polyclonal aptamers

Yuuya Kasahara, Shunsuke Kitadume, Kunihiko Morihiro, Masayasu Kuwahara, Hiroaki Ozaki, Hiroaki Sawai, Takeshi Imanishi, Satoshi Obika

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS　Vol. 20　No. 5　p. 1626-1629　2010/03　Research paper (scientific journal)

SYNTHESIS AND EVALUATION OF NOVEL 3,4-EPOXYPIPERIDINES AS EFFICIENT DNA ALKYLATING AGENTS

Yuji Kawada, Tetsuya Kodama, Kazuyuki Miyashita, Takeshi Imanishi, Satoshi Obika

HETEROCYCLES　Vol. 80　No. 2　p. 1249-1265　2010/03　Research paper (scientific journal)

Synthesis, RNA selective hybridization and high nuclease resistance of an oligonucleotide containing novel bridged nucleic acid with cyclic urea structure

Masaru Nishida, Takeshi Baba, Tetsuya Kodama, Aiko Yahara, Takeshi Imanishi, Satoshi Obika

CHEMICAL COMMUNICATIONS　Vol. 46　No. 29　p. 5283-5285　2010　Research paper (scientific journal)

2 ',4 '-BNA bearing a 2-pyridine nucleobase for CG base pair recognition in the parallel motif triplex DNA

Yoshiyuki Hari, Sachiko Matsugu, Hiroyasu Inohara, Yuri Hatanaka, Masaaki Akabane, Takeshi Imanishi, Satoshi Obika

ORGANIC & BIOMOLECULAR CHEMISTRY　Vol. 8　No. 18　p. 4176-4180　2010　Research paper (scientific journal)

A novel bridged nucleoside bearing a conformationally switchable sugar moiety in response to redox changes

Takeshi Baba, Tetsuya Kodama, Kazuto Mori, Takeshi Imanishi, Satoshi Obika

CHEMICAL COMMUNICATIONS　Vol. 46　No. 42　p. 8058-8060　2010　Research paper (scientific journal)

1P-094 Promotion of pyrimidine motif triplex formation at physiological pH by 2'-O,4'-C-aminomethylene bridged nucleic acid modification(Nucleic acid:Structure & Property, The 47th Annual Meeting of the Biophysical Society of Japan)

Sasaki Kiyomi, Sato Norihiro, Obika Satoshi, Imanishi Takeshi, Torigoe Hidetaka

Seibutsu Butsuri　Vol. 49　2009

Synthesis and properties of a novel 2',4'-BNA bearing a urea bridged structure.

Yahara, A., Nishida, M., Baba, T., Kodama, T., Imanishi, T., Obika, S.

Nucleic acids symposium series (2004)　No. 53　p. 11-12　2009　Research paper (scientific journal)

Synthesis and properties of a novel artificial nucleoside that acts as a redox switch.

Baba, T., Kodama, T., Imanishi, T., Obika, S.

Nucleic acids symposium series (2004)　No. 53　p. 107-108　2009　Research paper (scientific journal)

Promotion of triplex formation by 3'-amino-2'-O,4'-C-methylene bridged nucleic acid modification.

Sasaki, K., Rahman, S.M., Sato, N., Obika, S., Imanishi, T., Torigoe, H.

Nucleic acids symposium series (2004)　No. 53　p. 159-160　2009　Research paper (scientific journal)

Development of light-responsive BNA toward the spatiotemporal control of gene expression.

Morihiro, K., Kodama, T., Nishida, M., Imanishi, T., Obika, S.

Nucleic acids symposium series (2004)　No. 53　p. 187-188　2009　Research paper (scientific journal)

Design, synthesis, and properties of 2 ',4 '-BNA(NC): A bridged nucleic acid analogue

S. M. Abdur Rahman, Sayori Seki, Satoshi Obika, Haruhisa Yoshikawa, Kazuyuki Miyashita, Takeshi Imanishi

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY　Vol. 130　No. 14　p. 4886-4896　2008/04　Research paper (scientific journal)

2P-123 Promotion of Pyrimidine Motif Triplex Formation at Physiological PH by3'-Amino-2'-O, 4'-C-Methylene Bridged Nucleic Acid Modification(The 46th Annual Meeting of the Biophysical Society of Japan)

Sasaki Kiyomi, Obika Satoshi, Imanishi Takeshi, Torigoe Hidetaka

Seibutsu Butsuri　Vol. 48　2008

Design and chemical synthesis of a 2',4'-BNA probe for 7,8-dihydro-8-oxoguanine-containing double stranded DNA

Miyoshi, T., Kodama, T., Obika, S., Imanishi, T.

Nucleic acids symposium series (2004)　No. 52　p. 305-306　2008　Research paper (scientific journal)

Promotion of triplex formation by 2'-O,4'-C-aminomethylene bridged nucleic acid (2',4'-BNA NC) modification

Sasaki, K., Rahman, S.M., Obika, S., Imanishi, T., Torigoe, H.

Nucleic acids symposium series (2004)　No. 52　p. 419-420　2008　Research paper (scientific journal)

Biologically active 2-5A analogs containing 3'-O,4'-C-propylene adenosine as potent RNase L agonists

Morita, K., Obika, S., Imanishi, T., Kitade, Y., Koizumi, M.

Nucleic acids symposium series (2004)　No. 52　p. 637-638　2008　Research paper (scientific journal)

Promotion of acid-mediated P-N bond cleavage of 5 '-amino-2 ',4 '-BNA oligonucleotides by triplex formation: Effects of neighboring residues

Satoshi Obika, Masaharu Tomizu, Yoshinori Negoro, Ayako Orita, Takeshi Imanishi

CHEMISTRY OF NUCLEIC ACID COMPONENTS　Vol. 10　p. 423-425　2008　Research paper (international conference proceedings)

2P177 Rational combination of stabilizing strategies to achieve synergistic stabilization of nucleic acid assembly(36. DNA to chromatin,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)

Katayama Takuma, Maruyama Atsushi, Obika Satoshi, Imanishi Takeshi, Torigoe Hidetaka

Seibutsu Butsuri　Vol. 46　No. 2　2006

Antisense activity of 2',4'-BNA targeted to bcl-xL gene in HepG2 cell.

Roongjang, S., Takahashi, K., Park, M., Obika, S., Imanishi, T.

Nucleic acids symposium series (2004)　No. 50　p. 289-290　2006　Research paper (scientific journal)

Stereoselective synthesis of 3-alkylideneoxindoles via palladium-catalyzed domino reactions

R Yanada, S Obika, T Inokuma, K Yanada, M Yamashita, S Ohta, Y Takemoto

JOURNAL OF ORGANIC CHEMISTRY　Vol. 70　No. 17　p. 6972-6975　2005/08　Research paper (scientific journal)

Adjustment of the gamma dihedral angle of an oligonucleotide P3 -> N5 ' Phosphoramidate enhances its binding affinity towards complementary strands"

S Obika, M Sekiguchi, R Somjing, T Imanishi

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION　Vol. 44　No. 13　p. 1944-1947　2005　Research paper (scientific journal)

Promotion of acid-mediated cleavage of oligonucleotide P3'->N5' phosphoroamidates by triplex formation: a novel approach to sequence-specific DNA detection.

Tomizu, M., Nakagawa, O., Obika, S., Imanishi, T.

Nucleic acids symposium series (2004)　No. 49　p. 157-158　2005　Research paper (scientific journal)

Development of bridged nucleic acid analogues for antigene technology

S Obika

CHEMICAL & PHARMACEUTICAL BULLETIN　Vol. 52　No. 12　p. 1399-1404　2004/12

Synthesis and conformation of a novel bridged nucleic acid having a trans-fused 3,5,8-trioxabicyclo[5.3.0]decane structure

S Obika, T Osaki, M Sekiguchi, R Somjing, Y Harada, T Imanishi

TETRAHEDRON LETTERS　Vol. 45　No. 24　p. 4801-4804　2004/06　Research paper (scientific journal)

Synthesis and properties of a novel bridged nucleic acid analogue bearing a P3'-->N5' phosphoroamidate linkage, 5'-amino-3', 5'-BNA.

Sekiguchi, M., Obika, S., Somjing, R., Imanishi, T.

Nucleic acids symposium series (2004)　No. 48　p. 7-8　2004　Research paper (scientific journal)

2',4'-BNA derivatives bearing an unnatural nucleobase: synthesis and application to triplex-forming oligonucleotides.

Inohara, H., Obika, S., Imanishi, T.

Nucleic acids symposium series (2004)　No. 48　p. 63-64　2004　Research paper (scientific journal)

Conformations and dynamics of Ets-1 ETS domain-DNA complexes

SY Reddy, S Obika, TC Bruice

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　Vol. 100　No. 26　p. 15475-15480　2003/12　Research paper (scientific journal)

Sequence specific DNA binding of Ets-1 transcription factor: Molecular dynamics study on the Ets domain-DNA complexes

S Obika, SY Reddy, TC Bruice

JOURNAL OF MOLECULAR BIOLOGY　Vol. 331　No. 2　p. 345-359　2003/08　Research paper (scientific journal)

Triplex formation with 2 '-O,4 '-C-ethylene-bridged nucleic acids (ENA) having C3 '-endo conformation at physiological pH

M Koizumi, K Morita, M Daigo, S Tsutsumi, K Abe, S Obika, T Imanishi

NUCLEIC ACIDS RESEARCH　Vol. 31　No. 12　p. 3267-3273　2003/06　Research paper (scientific journal)

The unique target specificity of a nonpeptide chemokine receptor antagonist: selective blockade of two Th1 chemokine receptors CCR5 and CXCR3

P Gao, XY Zhou, Y Yashiro-Ohtani, YF Yang, N Sugimoto, S Ono, T Nakanishi, S Obika, T Imanishi, T Egawa, T Nagasawa, H Fujiwara, T Hamaoka

JOURNAL OF LEUKOCYTE BIOLOGY　Vol. 73　No. 2　p. 273-280　2003/02　Research paper (scientific journal)

Selective recognition of CG interruption by 2′,4′-BNA having 1-isoquinolone as a nucleobase in a pyrimidine motif triplex formation

Hari, Y., Obika, S., Sekiguchi, M., Imanishi, T.

Tetrahedron　Vol. 59　No. 27　p. 5123-5128　2003　Research paper (scientific journal)

Synthesis of a novel bridged nucleoside bearing a fused-azetidine ring, 3′-amino-3′,4′-BNA monomer

Obika, S., Andoh, J.-I., Onoda, M., Nakagawa, O., Hiroto, A., Sugimoto, T., Imanishi, T.

Tetrahedron Letters　Vol. 44　No. 28　p. 5267-5270　2003　Research paper (scientific journal)

Synthesis and properties of a novel bridged nucleic acid with a P3 '-> N5 ' phosphoramidate linkage, 5 '-amino-2 ',4 '-BNA

S Obika, O Nakagawa, A Hiroto, Y Hari, T Imanishi

CHEMICAL COMMUNICATIONS　No. 17　p. 2202-2203　2003　Research paper (scientific journal)

A novel nucleic acid analogue, 3'-amino-2'-deoxy-3',4'-BNA, with an N3'-->P5' phosphoramidate linkage and an S-type conformation.

Nakagawa, O., Hiroto, A., Obika, S., Imanishi, T.

Nucleic acids research. Supplement (2001)　No. 3　p. 87-88　2003　Research paper (scientific journal)

trans-3',4'-BNAs, novel nucleic acid analogues with an S-type conformation: synthesis and incorporation into oligonucleotides.

Sekiguchi, M., Osaki, T., Harada, Y., Obika, S., Imanishi, T.

Nucleic acids research. Supplement (2001)　No. 3　p. 111-112　2003　Research paper (scientific journal)

Triplex formation involving 2 '-O,4 '-C-methylene bridged nucleic acid (2 ',4 '-BNA) with 2-pyridone base analogue: Efficient and selective recognition of C : G interruption

H Torigoe, Y Hari, S Obika, T Imanishi

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS　Vol. 22　No. 5-8　p. 1097-1099　2003　Research paper (scientific journal)

Triplex formation involving 2 '-O,4 '-C-methylene bridged nucleic acid (2 ',4 '-BNA) with 1-isoquinolone base analogue: Efficient and selective recognition of C : G interruption

H Torigoe, Y Hari, S Obika, T Imanishi

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS　Vol. 22　No. 5-8　p. 1571-1573　2003　Research paper (scientific journal)

Stable oligonucleotide-directed triplex formation at target sites with CG interruptions: Strong sequence-specific recognition by 2 ',4 '-bridged nucleic-acid-containing 2-pyridones under physiological conditions

S Obika, Y Hari, M Sekiguchi, T Imanishi

CHEMISTRY-A EUROPEAN JOURNAL　Vol. 8　No. 20　p. 4796-4802　2002/10　Research paper (scientific journal)

BNAs: novel nucleic acid analogs with a bridged sugar moiety

T Imanishi, S Obika

CHEMICAL COMMUNICATIONS　No. 16　p. 1653-1659　2002/08　Research paper (scientific journal)

A non-peptide CCR5 antagonist inhibits collagen-induced arthritis by modulating T cell migration without affecting anti-collagen T cell responses

YF Yang, T Mukai, P Gao, N Yamaguchi, S Ono, H Iwaki, S Obika, T Imanishi, T Tsujimura, T Hamaoka, H Fujiwara

EUROPEAN JOURNAL OF IMMUNOLOGY　Vol. 32　No. 8　p. 2124-2132　2002/08　Research paper (scientific journal)

A pivotal role for CC chemokine receptor 5 in T-cell migration to tumor sites induced by interleukin 12 treatment in tumor-hearing mice

Y Uekusa, WG Yu, T Mukai, P Gao, N Yamaguchi, M Murai, K Matsushima, S Obika, T Imanishi, Y Higashibata, S Nomura, Y Kitamura, H Fujiwara, T Hamaoka

CANCER RESEARCH　Vol. 62　No. 13　p. 3751-3758　2002/07　Research paper (scientific journal)

A 3,4-epoxypiperidine structure as a novel and simple DNA-cleavage unit

K Miyashita, M Park, S Adachi, S Seki, S Obika, T Imanishi

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS　Vol. 12　No. 7　p. 1075-1077　2002/04　Research paper (scientific journal)

Synthesis and conformation of a novel bridged nucleoside with S-type sugar puckering, trans-3′,4′-BNA monomer

Obika, S., Sekiguchi, M., Osaki, T., Shibata, N., Masaki, M., Hari, Y., Imanishi, T.

Tetrahedron Letters　Vol. 43　No. 24　p. 4365-4368　2002　Research paper (scientific journal)

Synthesis and conformation of 3′,4′-BNA monomers, 3′-O,4′-C-methyleneribonucleosides

Obika, S., Morio, K.-I., Nanbu, D., Hari, Y., Itoh, H., Imanishi, T.

Tetrahedron　Vol. 58　No. 15　p. 3039-3049　2002　Research paper (scientific journal)

Effective synthesis of C-nucleosides with 2′,4′-BNA modification

Hari, Y., Obika, S., Sakaki, M., Morio, K.-I., Yamagata, Y., Imanishi, T.

Tetrahedron　Vol. 58　No. 15　p. 3051-3063　2002　Research paper (scientific journal)

Down-regulation of a gene-expression by an antisense BNA oligonucleotide

Takeshi Imanishi, Satoshi Obika

Folia Pharmacologica Japonica　Vol. 120　No. 2　p. 85-90　2002　Research paper (scientific journal)

Synthesis and properties of 5'-amino-2',4'-BNA modified oligonucleotides with P3'-->N5' phosphoramidate linkages.

Obika, S., Nakagawa, O., Hiroto, A., Hari, Y., Imanishi, T.

Nucleic acids research. Supplement (2001)　No. 2　p. 25-26　2002　Research paper (scientific journal)

Binding of threading intercalator to nucleic acids: thermodynamic analyses.

Torigoe H, Sato S, Yamashita K, Obika S, Imanishi T, Takenaka S

Nucleic acids research. Supplement (2001)　No. 2　p. 55-56　2002　Research paper (scientific journal)

Synthesis and properties of oligonucleotides containing novel 2',4'-BNA analogues (2',4'-BNACOC).

Hari Y, Osaki T, Eguchi K, Obika S, Imanishi T

Nucleic acids research. Supplement (2001)　Vol. 2　No. 2　p. 147-148　2002

Triplex formation involving 2',4'-BNA with isoquinolone base analogue: efficient and selective recognition of C:G interruption.

Torigoe, H., Hari, Y., Obika, S., Imanishi, T.

Nucleic acids research. Supplement (2001)　No. 2　p. 183-184　2002　Research paper (scientific journal)

3 '-Amino-2 ',4 '-BNA: novel bridged nucleic acids having an N3 '-> P5 ' phosphoramidate linkage

S Obika, M Onoda, K Morita, J Andoh, M Koizumi, T Imanishi

CHEMICAL COMMUNICATIONS　No. 19　p. 1992-1993　2001/10　Research paper (scientific journal)

Promotion of triplex formation by 2′-O,4′-C-methylene bridged nucleic acid (2′,4′-BNA) modification: Thermodynamic and kinetic studies

Torigoe, H., Obika, S., Imanishi, T.

Nucleosides, Nucleotides and Nucleic Acids　Vol. 20　No. 4-7　2001/04　Research paper (scientific journal)

Triplex formation involving 2',4'-BNA with 2-pyridone base analogue: efficient and selective recognition of C:G interruption.

Torigoe, H., Hari, Y., Obika, S., Imanishi, T.

Nucleic acids research. Supplement (2001)　No. 1　p. 281-282　2001　Research paper (scientific journal)

2',4'-BNA bearing unnatural nucleobases: toward the expansion of the target sequence of double-stranded DNA in triplex formation.

Obika S, Hari Y, Inohara H, Imanishi T

Nucleic acids research. Supplement (2001)　Vol. 1　No. 1　p. 171-172　2001

A 2 ',4 '-bridged nucleic acid containing 2-pyridone as a nucleobase: Efficient recognition of a C center dot G interruption by tripler formation with a pyrimidine motif

S Obika, Y Hari, M Sekiguchi, T Imanishi

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION　Vol. 40　No. 11　p. 2079-2081　2001　Research paper (scientific journal)

TRANSDERMAL PENETRATION-ENHANCING EFFECT AND ITS MECHANISM OF NOVEL TRIGLYCERIDES HAVING QUATERNARY AMMONIUM GROUP

KUBO Kazuyoshi, TOJYO Nobuteru, YU Wei, OBIKA Satoshi, IMANISHI Takeshi, MAYUMI Tadanori

Drug Metabolism and Pharmacokinetics　Vol. 9　p. 86-89　1994