Angucycline antibiotic waldiomycin recognizes common structural motif conserved in bacterial histidine kinases

Yoko Eguchi, Toshihide Okajima, Naoya Tochio, Yoichi Inukai, Riko Shimizu, Shuhei Ueda, Shoko Shinya, Takanori Kigawa, Tamo Fukamizo, Masayuki Igarashi, Ryutaro Utsumi

JOURNAL OF ANTIBIOTICS　Vol. 70　No. 3　p. 251-258　2017/03　Research paper (scientific journal)

Probing the Catalytic Mechanism of Copper Amine Oxidase from Arthrobacter globiformis with Halide Ions

Takeshi Murakawa, Akio Hamaguchi, Shota Nakanishi, Misumi Kataoka, Tadashi Nakai, Yoshiaki Kawano, Hiroshi Yamaguchi, Hideyuki Hayashi, Katsuyuki Tanizawa, Toshihide Okajima

JOURNAL OF BIOLOGICAL CHEMISTRY　Vol. 290　No. 38　p. 23094-23109　2015/09　Research paper (scientific journal)

The Radical S-Adenosyl-L-methionine Enzyme QhpD Catalyzes Sequential Formation of Intra-protein Sulfur-to-Methylene Carbon Thioether Bonds

Tadashi Nakai, Hiroto Ito, Kazuo Kobayashi, Yasuhiro Takahashi, Hiroshi Hori, Motonari Tsubaki, Katsuyuki Tanizawa, Toshihide Okajima

JOURNAL OF BIOLOGICAL CHEMISTRY　Vol. 290　No. 17　p. 11144-11166　2015/04　Research paper (scientific journal)

Trapping of a dopaquinone intermediate in the TPQ cofactor biogenesis in a copper-containing amine oxidase from Arthrobacter globiformis

Robyn H. Moore, M. Ashley Spies, Matthew B. Culpepper, Takeshi Murakawa, Shun Hirota, Toshihide Okajima, Katsuyuki Tanizawa, Minae Mure

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY　Vol. 129　No. 37　p. 11524-11534　2007/09　Research paper (scientific journal)

Role of copper ion in bacterial copper amine oxidase: Spectroscopic and crystallographic studies of metal-substituted enzymes

Kishishita, S, Okajima, T, Kim, M, Yamaguchi, H, Hirota, S, Suzuki, S, Kuroda, S, Tanizawa, K, Mure, M

J. Am. Chem. Soc　Vol. 125　No. 4　2003/01/29

X-ray snapshots of quinone cofactor biogenesis in bacterial copper amine oxidase

M Kim, T Okajima, S Kishishita, M Yoshimura, A Kawamori, K Tanizawa, H Yamaguchi

NATURE STRUCTURAL BIOLOGY　Vol. 9　No. 8　p. 591-596　2002/08　Research paper (scientific journal)

Crystal structure of quinohemoprotein amine dehydrogenase from Pseudomonas putida - Identification of a novel quinone cofactor encaged by multiple thioether cross-bridges

A Satoh, JK Kim, Miyahara, I, B Devreese, Vandenberghe, I, A Hacisalihoglu, T Okajima, S Kuroda, O Adachi, JA Duine, J Van Beeumen, K Tanizawa, K Hirotsu

JOURNAL OF BIOLOGICAL CHEMISTRY　Vol. 277　No. 4　p. 2830-2834　2002/01　Research paper (scientific journal)

Structure of a quinohemoprotein amine dehydrogenase with an uncommon redox cofactor and highly unusual crosslinking

S Datta, Y Mori, K Takagi, K Kawaguchi, ZW Chen, T Okajima, S Kuroda, T Ikeda, K Kano, K Tanizawa, FS Mathews

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　Vol. 98　No. 25　p. 14268-14273　2001/12　Research paper (scientific journal)

SpyRing-Mediated Cyclization of TEV Protease, Guided by AlphaFold, Improves Thermostability

Tadashi Nakai, Yota Nakai, Naoki Takami, Emi Nakai, Toshihide Okajima

ACS Omega　2025/07/25　Research paper (scientific journal)

Metabolic role of a genetically conserved aldehyde dehydrogenase in bacterial assimilation of various primary amines

Tadashi Nakai, Naoya Miyagi, Kota Hisamura, Shoya Matsuba, Kanji Nishimoto, Emi Nakai, Katsuyuki Tanizawa, Toshihide Okajima

Archives of Biochemistry and Biophysics　Vol. 768　p. 110387-110387　2025/06　Research paper (scientific journal)

Neutron Crystallography of a Semiquinone Radical Intermediate of Copper Amine Oxidase

T. Murakawa, K. Kurihara, K. Kusaka, M. Adachi, T. Okajima

Vol. 24　No. 2　p. 7-9　2025/01

New Generation of Antibacterial Drugs to Inhibit Bacterial Signal Transduction: Can Inhibitors Against Two-Component Systems Be a Breakthrough in Development of Drugs to Defeat Drug Resistant Bacteria?

Toshihide OKAJIMA, Teruhiko ISHIKAWA, Masayuki IGARASHI, Yoko EGUCHI, Ryutaro UTSUMI

Vol. 62　p. 480-489　2024/10　Research paper (scientific journal)

Unravelling nicotinic receptor and ligand features underlying neonicotinoid knockdown actions on the malaria vector mosquito Anopheles gambiae

Ryo Ito, Masaki Kamiya, Koichi Takayama, Sumito Mori, Rei Matsumoto, Mayuka Takebayashi, Hisanori Ojima, Shota Fujimura, Haruki Yamamoto, Masayuki Ohno, Makoto Ihara, Toshihide Okajima, Atsuko Yamashita, Fraser Colman, Gareth J. Lycett, David B. Sattelle, Kazuhiko Matsuda

Open Biology　Vol. 14　No. 7　2024/07/24　Research paper (scientific journal)

Synthesis and biochemical characterization of naphthoquinone derivatives targeting bacterial histidine kinases

Teruhiko Ishikawa, Yoko Eguchi, Masayuki Igarashi, Toshihide Okajima, Kohei Mita, Yuri Yamasaki, Kaho Sumikura, Taisei Okumura, Yuna Tabuchi, Chigusa Hayashi, Martina Pasqua, Marco Coluccia, Gianni Prosseda, Bianca Colonna, Chie Kohayakawa, Akiyoshi Tani, Jun-ichi Haruta, Ryutaro Utsumi

The Journal of Antibiotics　Vol. 77　No. 8　p. 522-532　2024/06/25　Research paper (scientific journal)

Serial femtosecond X-ray crystallography of an anaerobically formed catalytic intermediate of copper amine oxidase

Takeshi Murakawa, Mamoru Suzuki, Kenji Fukui, Tetsuya Masuda, Michihiro Sugahara, Kensuke Tono, Tomoyuki Tanaka, So Iwata, Eriko Nango, Takato Yano, Katsuyuki Tanizawa, Toshihide Okajima

Acta Crystallographica Section D Structural Biology　Vol. 78　No. 12　p. 1428-1438　2022/12/01　Research paper (scientific journal)

Roles of Two-Component Signal Transduction Systems in Shigella Virulence

Martina Pasqua, Marco Coluccia, Yoko Eguchi, Toshihide Okajima, Milena Grossi, Gianni Prosseda, Ryutaro Utsumi, Bianca Colonna

Biomolecules　Vol. 12　No. 9　p. 1321-1321　2022/09/18　Research paper (scientific journal)

Molecular mechanism of a large conformational change of the quinone cofactor in the semiquinone intermediate of bacterial copper amine oxidase

Mitsuo Shoji, Takeshi Murakawa, Shota Nakanishi, Mauro Boero, Yasuteru Shigeta, Hideyuki Hayashi, Toshihide Okajima

Chemical Science　Vol. 13　No. 36　p. 10923-10938　2022/08/23　Research paper (scientific journal)

Re-evaluation of protein neutron crystallography with and without X-ray/neutron joint refinement

Takeshi Murakawa, Kazuo Kurihara, Motoyasu Adachi, Katsuhiro Kusaka, Katsuyuki Tanizawa, Toshihide Okajima

IUCrJ　Vol. 9　No. 3　p. 342-348　2022/05/01　Research paper (scientific journal)

キノン補酵素形成に関わる新奇フラビン酵素の構造と機能

Journal of Japanese Biochemical Society　Vol. 94　No. 1　p. 92-96　2022/01

Neutron Crystallography Reveals Unprecedented Active-site Structure in Copper Amine Oxidase

T. Murakawa, K. Kurihara, T. Tamada, K. Kusaka, M. Adachi, T. Okajima

J-PARC MLF ANNUAL REPORT 2020　Vol. 12　p. 14-16　2022/01

Microcrystal preparation for serial femtosecond X-ray crystallography of bacterial copper amine oxidase

Takeshi Murakawa, Mamoru Suzuki, Toshi Arima, Michihiro Sugahara, Tomoyuki Tanaka, Rie Tanaka, So Iwata, Eriko Nango, Kensuke Tono, Hideyuki Hayashi, Kenji Fukui, Takato Yano, Katsuyuki Tanizawa, Toshihide Okajima

Acta Crystallographica Section F Structural Biology Communications　Vol. 77　No. 10　p. 356-363　2021/10/01　Research paper (scientific journal)

X-Ray and Neutron Joint Refinement Displays Shared Proton and Keto Form of Quinone Cofactor in Copper Amine Oxidase

T. Murakawa, T. Okajima, K. Kurihara, M. Adachi

Photon Factory Highlights 2020　Vol. 6　p. 42-43　2021/07

Eight genes are necessary and sufficient for biogenesis of Quinohemoprotein amine dehydrogenase

Tadashi Nakai, Katsuyuki Tanizawa, Toshihide Okajima

Bioscience, Biotechnology, and Biochemistry　2021/06/30　Research paper (scientific journal)

Acid-Sensing Histidine Kinase With a Redox Switch

Shinya Inada, Toshihide Okajima, Ryutaro Utsumi, Yoko Eguchi

Frontiers in Microbiology　Vol. 12　2021/05/20　Research paper (scientific journal)

L-メチオニン脱炭酸酵素を用いた新規L-メチオニン定量法の開発とL-ホモシステイン定量への応用

大川 敦司, 林 将也, 稲垣 純子, 岡島 俊英, 田村 隆, 稲垣 賢二

ビタミン　Vol. 94　No. 11　p. 549-552　2020/11　Research paper (scientific journal)

Unique protonation states of aspartate and topaquinone in the active site of copper amine oxidase

Mitsuo Shoji, Takeshi Murakawa, Mauro Boero, Yasuteru Shigeta, Hideyuki Hayashi, Toshihide Okajima

RSC Advances　Vol. 10　No. 63　p. 38631-38639　2020/10/21　Research paper (scientific journal)

In crystallo thermodynamic analysis of the catalytic reaction in bacterial copper amine oxidase

Takeshi Murakawa, Seiki Baba, Toshihide Okajima

SPring-8/SACLA Research Frontiers 2019　p. 20-21　2020/08　Research paper (bulletin of university, research institution)

An immuno-dot blot assay for screening histidine kinase inhibitors

Emiko Kinoshita-Kikuta, Shino Maruta, Yoko Eguchi, Masayuki Igarashi, Toshihide Okajima, Ryutaro Utsumi, Eiji Kinoshita, Tohru Koike

Analytical Biochemistry　Vol. 600　p. 113765-113765　2020/07　Research paper (scientific journal)

Novel method for l-methionine determination using l-methionine decarboxylase and application of the enzyme for l-homocysteine determination.

Atsushi Okawa, Masaya Hayashi, Junko Inagaki, Toshihide Okajima, Takashi Tamura, Kenji Inagaki

Bioscience, biotechnology, and biochemistry　Vol. 0916-8451 (print)　No. 1347-6947 (online)　p. 1-9　2020/01/20　Research paper (scientific journal)

Angucycline抗菌化合物のヒスチジンキナーゼ結合部位の探索

岡島俊英, 犬飼洋一, 長尾勇希, 江口陽子, 石川彰彦, 五十嵐雅之, 内海龍太郎

SPring-8/SACLA利用研究成果集 Section A　p. 79-83　2020/01　Research paper (scientific journal)

Quantitative monitoring of His and Asp phosphorylation in a bacterial signaling system by using Phos-tag Magenta/Cyan fluorescent dyes

E. Kinoshita‐Kikuta, H. Kusamoto, S. Ono, K. Akayama, Y. Eguchi, M. Igarashi, T. Okajima, R. Utsumi, E. Kinoshita, T. Koike

Electrophoresis　Vol. 40　No. 22　p. 3005-3013　2019/11　Research paper (scientific journal)

銅含有アミン酸化酵素触媒反応におけるコンフォメーション変化のin crystallo熱力学解析

村川武志, 馬場清喜, 岡島俊英

生化学　Vol. 91　No. 4　p. 565-571　2019/08

放線菌Streptomyces sp.590由来の抗腫瘍性酵素L‐メチオニン脱炭酸酵素の遺伝子クローニング,組換え発現,均一精製および性質検討

林将也, 岡田茜, 山本久美子, 奥河内知美, 日下知香, 工藤大蔵, 根本理子, 稲垣純子, 広瀬侑, 岡島俊英, 田村隆, 左右田健次, 稲垣賢二

ビタミン　Vol. 92　No. 3　p. 113-116　2018/03/25　Research paper (scientific journal)

Gene cloning, recombinant expression, purification and characterization of L-methionine decarboxylase from Streptomyces sp 590

Masaya Hayashi, Akane Okada, Kumiko Yamamoto, Tomomi Okugochi, Chika Kusaka, Daizou Kudou, Michiko Nemoto, Junko Inagaki, Yuu Hirose, Toshihide Okajima, Takashi Tamura, Kenji Soda, Kenji Inagaki

JOURNAL OF BIOCHEMISTRY　Vol. 161　No. 4　p. 389-398　2017/04　Research paper (scientific journal)

Characterization of H-box region mutants of WalK inert to the action of waldiomycin in Bacillus subtilis

Akinori Kato, Shuhei Ueda, Taku Oshima, Yoichi Inukai, Toshihide Okajima, Masayuki Igarashi, Yoko Eguchi, Ryutaro Utsumi

JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY　Vol. 63　No. 4　p. 212-221　2017　Research paper (scientific journal)

Cellular uptake of hepatitis B virus envelope L particles is independent of sodium taurocholate cotransporting polypeptide, but dependent on heparan sulfate proteoglycan

Masaharu Somiya, Qiushi Liu, Nobuo Yoshimoto, Masumi Iijima, Kenji Tatematsu, Tadashi Nakai, Toshihide Okajima, Kazuyuki Kuroki, Keiji Ueda, Shun'ichi Kuroda

VIROLOGY　Vol. 497　p. 23-32　2016/10　Research paper (scientific journal)

Cytokine-Dependent Activation of JAK-STAT Pathway in Saccharomyces cerevisiae

Nobuo Yoshimoto, Yuko Ikeda, Kenji Tatematsu, Masumi Iijima, Tadashi Nakai, Toshihide Okajima, Katsuyuki Tanizawa, Shun'ichi Kuroda

BIOTECHNOLOGY AND BIOENGINEERING　Vol. 113　No. 8　p. 1796-1804　2016/08　Research paper (scientific journal)

Mutational analysis of hepatitis B virus pre-S1 (9-24) fusogenic peptide

Qiushi Liu, Masaharu Somiya, Naohiko Shimada, Wakako Sakamoto, Nobuo Yoshimoto, Masumi Iijima, Kenji Tatematsu, Tadashi Nakai, Toshihide Okajima, Atsushi Maruyama, Shunichi Kuroda

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　Vol. 474　No. 2　p. 406-412　2016/05　Research paper (scientific journal)

Bio-nanocapsules displaying various immunoglobulins as an active targeting-based drug delivery system

Kenji Tatematsu, Masumi Iijima, Nobuo Yoshimoto, Tadashi Nakai, Toshihide Okajima, Shun'ichi Kuroda

ACTA BIOMATERIALIA　Vol. 35　p. 238-247　2016/04　Research paper (scientific journal)

Studies on an Acetylcholine Binding Protein Identify a Basic Residue in Loop G on the beta 1 Strand as a New Structural Determinant of Neonicotinoid Actions

Makoto Ihara, Toshihide Okajima, Atsuko Yamashita, Takuma Oda, Takuya Asano, Mikana Matsui, David B. Sattelle, Kazuhiko Matsuda

MOLECULAR PHARMACOLOGY　Vol. 86　No. 6　p. 736-746　2014/12　Research paper (scientific journal)

Identification of Genes Essential for the Biogenesis of Quinohemoprotein Amine Dehydrogenase

Tadashi Nakai, Takafumi Deguchi, Ivo Frebort, Katsuyuki Tanizawa, Toshihide Okajima

BIOCHEMISTRY　Vol. 53　No. 5　p. 895-907　2014/02　Research paper (scientific journal)

High-resolution crystal structure of copper amine oxidase from Arthrobacter globiformis: assignment of bound diatomic molecules as O-2

Takeshi Murakawa, Hideyuki Hayashi, Tomoko Sunami, Kazuo Kurihara, Taro Tamada, Ryota Kuroki, Mamoru Suzuki, Katsuyuki Tanizawa, Toshihide Okajima

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY　Vol. 69　p. 2483-2494　2013/12　Research paper (scientific journal)

Isolation and Characterization of Signermycin B, an Antibiotic That Targets the Dimerization Domain of Histidine Kinase WalK

Takafumi Watanabe, Masayuki Igarashi, Toshihide Okajima, Eiji Ishii, Hirokazu Kino, Masaki Hatano, Ryuichi Sawa, Maya Umekita, Tomoyuki Kimura, Sho Okamoto, Yoko Eguchi, Yuzuru Akamatsu, Ryutaro Utsumi

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY　Vol. 56　No. 7　p. 3657-3663　2012/07　Research paper (scientific journal)

Structural insights into the substrate specificity of bacterial copper amine oxidase obtained by using irreversible inhibitors

Takeshi Murakawa, Hideyuki Hayashi, Masayasu Taki, Yukio Yamamoto, Yoshiaki Kawano, Katsuyuki Tanizawa, Toshihide Okajima

JOURNAL OF BIOCHEMISTRY　Vol. 151　No. 2　p. 167-178　2012/02　Research paper (scientific journal)

An Unusual Subtilisin-like Serine Protease Is Essential for Biogenesis of Quinohemoprotein Amine Dehydrogenase

Tadashi Nakai, Kazutoshi Ono, Shun'ichi Kuroda, Katsuyuki Tanizawa, Toshihide Okajima

JOURNAL OF BIOLOGICAL CHEMISTRY　Vol. 287　No. 9　p. 6530-6538　2012/02　Research paper (scientific journal)

A short-chain dehydrogenase involved in terpene metabolism from Zingiber zerumbet

Sho Okamoto, Fengnian Yu, Hisashi Harada, Toshihide Okajima, Jun-ichiro Hattan, Norihiko Misawa, Ryutaro Utsumi

FEBS JOURNAL　Vol. 278　No. 16　p. 2892-2900　2011/08　Research paper (scientific journal)

銅含有アミン酸化酵素の酸塩基触媒反応機構(第424回研究協議会研究発表要旨,ビタミンB研究委員会)

村川 武志, 林 秀行, 岡島 俊英, 谷澤 克行

ビタミン　Vol. 85　No. 8　p. 448-449　2011　Research paper (conference, symposium, etc.)

Detection of the reaction intermediates catalyzed by a copper amine oxidase

Misumi Kataoka, Hiroko Oya, Ayuko Tominaga, Masayuki Otsu, Toshihide Okajima, Katsuyuki Tanizawa, Hiroshi Yamaguchi

JOURNAL OF SYNCHROTRON RADIATION　Vol. 18　p. 58-61　2011/01　Research paper (scientific journal)

X-Ray Crystal Structure of the DNA-Binding Domain of Response Regulator WalR Essential to the Cell Viability of Staphylococcus aureus and Interaction with Target DNA

Akihiro Doi, Toshihide Okajima, Yasuhiro Gotoh, Katsuyuki Tanizawa, Ryutaro Utsumi

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY　Vol. 74　No. 9　p. 1901-1907　2010/09　Research paper (scientific journal)

Novel antibacterial compounds specifically targeting the essential WalR response regulator

Yasuhiro Gotoh, Akihiro Doi, Eiji Furuta, Sarah Dubrac, Yoshimasa Ishizaki, Masato Okada, Masayuki Igarashi, Norihiko Misawa, Hirofumi Yoshikawa, Toshihide Okajima, Tarek Msadek, Ryutaro Utsumi

JOURNAL OF ANTIBIOTICS　Vol. 63　No. 3　p. 127-134　2010/03　Research paper (scientific journal)

Walkmycin B targets WalK (YycG), a histidine kinase essential for bacterial cell growth

Ario Okada, Masayuki Igarashi, Toshihide Okajima, Naoko Kinoshita, Maya Umekita, Ryuichi Sawa, Kunio Inoue, Takafumi Watanabe, Akihiro Doi, Aaron Martin, John Quinn, Yoshio Nishimura, Ryutaro Utsumi

JOURNAL OF ANTIBIOTICS　Vol. 63　No. 2　p. 89-94　2010/02　Research paper (scientific journal)

Accumulation of polyubiquitinated proteins by overexpression of RBCC protein interacting with protein kinase C2, a splice variant of ubiquitin ligase RBCC protein interacting with protein kinase C1

Nobuo Yoshimoto, Kenji Tatematsu, Toshihide Okajima, Katsuyuki Tanizawa, Shun'ichi Kuroda

FEBS JOURNAL　Vol. 276　No. 21　p. 6375-6385　2009/11　Research paper (scientific journal)

3P-002 X-ray crystal structure analysis of reaction intermediate of Copper and TPQ containing Amine oxidase from Arthrobacter globiformis(Protein:Structure,The 47th Annual Meeting of the Biophysical Society of Japan)

Kataoka Misumi, Tominaga Ayuko, Ohtsu Masayuki, Okajima Toshihide, Tanizawa Katsuyuki, Yamaguchi Hiroshi

Seibutsu Butsuri　Vol. 49　2009　Research paper (conference, symposium, etc.)

分子内架橋構造をもちトリプトファン残基に由来するキノン補酵素の生合成機構

岡島 俊英, 谷澤 克行

化学と生物　Vol. 47 (8), 522–524　No. 8　p. 522-524　2009

Expression of squamous cell carcinoma antigen-1 in liver enhances the uptake of hepatitis B virus envelope-derived bio-nanocapsules in transgenic rats

Takeshi Kasuya, Shintaro Nomura, Takashi Matsuzaki, Joohee Jung, Tadanori Yamada, Kenji Tatematsu, Toshihide Okajima, Katsuyuki Tanizawa, Shun'ichi Kuroda

FEBS JOURNAL　Vol. 275　No. 22　p. 5714-5724　2008/11　Research paper (scientific journal)

Response regulator YycF essential for bacterial growth: X-ray crystal structure of the DNA-binding domain and its PhoB-like DNA recognition motif

Toshihide Okajima, Akihiro Doi, Ario Okada, Yasuhiro Gotoh, Katsuyuki Tanizawa, Ryutaro Utsumi

FEBS LETTERS　Vol. 582　No. 23-24　p. 3434-3438　2008/10　Research paper (scientific journal)

In vivo delivery of bionanocapsules displaying Phaseolus vulgaris agglutinin-L(4) isolectin to malignant tumors overexpressing N-acetylglucosaminyltransferase V

Takeshi Kasuya, Joohee Jung, Hiroyasu Kadoya, Takashi Matsuzaki, Kenji Tatematsu, Toshihide Okajima, Eiji Miyoshi, Katsuyuki Tanizawa, Shun'ichi Kuroda

HUMAN GENE THERAPY　Vol. 19　No. 9　p. 887-895　2008/09　Research paper (scientific journal)

Further insight into the mechanism of stereoselective proton abstraction by bacterial copper amine oxidase

Masayasu Taki, Takeshi Murakawa, Takuya Nakamoto, Mayuni Uchida, Hideyuki Hayashi, Katsuyuki Tanizawa, Yukio Yamamoto, Toshihide Okajima

BIOCHEMISTRY　Vol. 47　No. 29　p. 7726-7733　2008/07　Research paper (scientific journal)

In vivo protein delivery to human liver-derived cells using hepatitis B virus envelope pre-S region

Takeshi Kasuya, Tadanori Yamada, Atsuko Uyeda, Takashl Matsuzaki, Toshihide Okajima, Kenji Tatematsu, Katsuyuki Tanizawa, Shun'ichi Kuroda

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　Vol. 106　No. 1　p. 99-102　2008/07　Research paper (scientific journal)

Crystal structures of Lymnaea stagnalis AChBP in complex with neonicotinoid insecticides imidacloprid and clothianidin

Makoto Ihara, Toshihide Okajima, Atsuko Yamashita, Takuma Oda, Koichi Hirata, Hisashi Nishiwaki, Takako Morimoto, Miki Akamatsu, Yuji Ashikawa, Shun'Ichi Kuroda, Ryosuke Mega, Seiki Kuramitsu, David B. Sattelle, Kazuhiko Matsuda

Invertebrate Neuroscience　Vol. 8　No. 2　p. 71-81　2008/06　Research paper (scientific journal)

Identification of Ubiquitin Ligase Activity of RBCK1 and Its Inhibition by Splice Variant RBCK2 and Protein Kinase Cβ

Kenji Tatematsu, Nobuo Yoshimoto, Toshihide Okajima, Katsuyuki Tanizawa, Shun'ichi Kuroda

Journal of Biological Chemistry　Vol. 283　No. 17　p. 11575-11585　2008/04/25　Research paper (scientific journal)

Efficient bacterial production of functional antibody fragments using a phagemid vector

Hiroyoshi Kuba, Atsuko Furukawa, Toshihide Okajima, Koji Furukawa

PROTEIN EXPRESSION AND PURIFICATION　Vol. 58　No. 2　p. 292-300　2008/04　Research paper (scientific journal)

Bio-nanocapsule conjugated with liposomes for in vivo pinpoint delivery of various materials

Joohee Jung, Takashi Matsuzaki, Kenji Tatematsu, Toshihide Okajima, Katsuyuki Tanizawa, Shun'ichi Kuroda

JOURNAL OF CONTROLLED RELEASE　Vol. 126　No. 3　p. 255-264　2008/03　Research paper (scientific journal)

1P-236 Crystal structures of an acetylcholine binding protein complexed with neonicotinoids(The 46th Annual Meeting of the Biophysical Society of Japan)

Ihara Makoto, Okajima Toshihide, Yamashita Atsuko, Oda Takuma, Ashikawa Yuji, Sattelle David B., Matsuda Kazuhiko

Seibutsu Butsuri　Vol. 48　2008　Research paper (conference, symposium, etc.)

Involvement of MAPK signaling molecules and Runx2 in the NELL1-induced osteoblastic differentiation

Nobuyuki Bokui, Takayuki Otani, Koichi Igarashi, Junichiro Kaku, Mitsuo Oda, Tadahiro Nagaoka, Masaharu Seno, Kenji Taternatsu, Toshihide Okajima, Takashi Matsuzaki, Kang Ting, Katsuyuki Tanizawa, Shunichi Kuroda

FEBS LETTERS　Vol. 582　No. 2　p. 365-371　2008/01　Research paper (scientific journal)

Axonal guidance protein FEZ1 associates with tubulin and kinesin motor protein to transport mitochondria in neurites of NGF-stimulated PC12 cells

Toshitsugu Ftijita, Andres D. Maturana, Junko Ikuta, Juri Hamada, Sebastien Walchli, Tadaki Suzuki, Hirofurni Sawa, Marie W. Wooten, Toshihide Okajima, Kenji Taternatsu, Katsuyuki Tanizawa, Shun'ichi Kuroda

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　Vol. 361　No. 3　p. 605-610　2007/09　Research paper (scientific journal)

Fasciculation and elongation protein zeta-1 (FEZ1) participates in the polarization of hippocampal neuron by controlling the mitochondrial motility

Junko Ikuta, Andres Maturana, Toshitsugu Fujita, Toshihide Okajima, Kenji Tatematsu, Katsuyuki Tanizawa, Shun'ichi Kuroda

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　Vol. 353　No. 1　p. 127-132　2007/02　Research paper (scientific journal)

Conformational changes during apoplastocyanin folding observed by photocleavable modification and transient grating

S Hirota, Y Fujimoto, J Choi, N Baden, N Katagiri, M Akiyama, R Hulsker, M Ubbink, T Okajima, T Takabe, N Funasaki, Y Watanabe, M Terazima

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY　Vol. 128　No. 23　p. 7551-7558　2006/06　Research paper (scientific journal)

Involvement of a putative [Fe-S]-cluster-binding protein in the biogenesis of quinohemoprotein amine dehydrogenase

K Ono, T Okajima, M Tani, S Kuroda, D Sun, VL Davidson, K Tanizawa

JOURNAL OF BIOLOGICAL CHEMISTRY　Vol. 281　No. 19　p. 13672-13684　2006/05　Research paper (scientific journal)

Quantum mechanical hydrogen tunneling in bacterial copper amine oxidase reaction

T Murakawa, T Okajima, S Kuroda, T Nakamoto, M Taki, Y Yamamoto, H Hayashi, K Tanizawa

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　Vol. 342　No. 2　p. 414-423　2006/04　Research paper (scientific journal)

Initial repertoire of anti-(4-hydroxy-3-nitrophenylacetyl) antibodies as potential donors for effective affinity maturation

K Furukawa, A Manabe, A Furukawa, H Kuba, T Okajima, T Azuma

MOLECULAR IMMUNOLOGY　Vol. 43　No. 11　p. 1751-1760　2006/04　Research paper (scientific journal)

Kinetic and structural studies on the catalytic role of the aspartic acid residue conserved in copper amine oxidase

YC Chiu, T Okajima, T Murakawa, M Uchida, M Taki, S Hirota, M Kim, H Yamaguchi, Y Kawano, N Kamiya, S Kuroda, H Hayashi, Y Yamamoto, K Tanizawa

BIOCHEMISTRY　Vol. 45　No. 13　p. 4105-4120　2006/04　Research paper (scientific journal)

Crystal structures of cytochrome c(L) and methanol dehydrogenase from Hyphomicrobium denitrificans: Structural and mechanistic insights into interactions between the two proteins

M Nojiri, D Hira, K Yamaguchi, T Okajima, K Tanizawa, S Suzuki

BIOCHEMISTRY　Vol. 45　No. 11　p. 3481-3492　2006/03　Research paper (scientific journal)

1P104 Conformational Changes during Apoplastocyanin Folding Observed by Photocleavable Modification and Transient Grating(3. Protein folding and misfolding (I),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)

Hirota Shun, Fujimoto Yukari, Choi Jungkwon, Baden Naoki, Katagiri Noriko, Akiyama Masako, Hulsker Rinske, Ubbink Marcellus, Okajima Toshihide, Takabe Teruhiro, Funasaki Noriaki, Watanabe Yoshihito, Terazima Masahide

Seibutsu Butsuri　Vol. 46　No. 2　2006

X線結晶解析と反応速度論的解析に基づく銅アミン酸化酵素の触媒機構

日本応用酵素協会誌　Vol. 41, 1-7　2006

Cytoplasmic tethering of a RING protein RBCK1 by its splice variant lacking the RING domain

N Yoshimoto, K Tatematsu, T Koyanagi, T Okajima, K Tanizawa, S Kuroda

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　Vol. 335　No. 2　p. 550-557　2005/09　Research paper (scientific journal)

Reinvestigation of metal ion specificity for quinone cofactor biogenesis in bacterial copper amine oxidase

T Okajima, S Kishishita, YC Chiu, T Murakawa, M Kim, H Yamaguchi, S Hirota, S Kuroda, K Tanizawa

BIOCHEMISTRY　Vol. 44　No. 36　p. 12041-12048　2005/09　Research paper (scientific journal)

Preparation and characterization of Ca2+-free methanol dehydrogenase from Hyphomicrobium denitrificans A3151

M Nojiri, D Hira, K Yamaguchi, T Okajima, K Tanizawa, S Suzuki

CHEMISTRY LETTERS　Vol. 34　No. 7　p. 1036-1037　2005/07　Research paper (scientific journal)

Nuclear-cytoplasmic shuttling of a RING-IBR protein RBCK1 and its functional interaction with nuclear body proteins

K Tatematsu, N Yoshimoto, T Koyanagi, C Tokunaga, T Tachibana, Y Yoneda, M Yoshida, T Okajima, K Tanizawa, S Kuroda

JOURNAL OF BIOLOGICAL CHEMISTRY　Vol. 280　No. 24　p. 22937-22944　2005/06　Research paper (scientific journal)

PKC epsilon-PKD1 signaling complex at Z-discs plays a pivotal role in the cardiac hypertrophy induced by G-protein coupling receptor agonists

M Iwata, A Maturana, M Hoshijima, K Tatematsu, T Okajima, Vandenheede, JR, J Van Lint, K Tanizawa, S Kuroda

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　Vol. 327　No. 4　p. 1105-1113　2005/02　Research paper (scientific journal)

Chemical rescue of a site-specific mutant of bacterial copper amine oxidase for generation of the topa quinone cofactor

Matsunami, H, Okajima, T, Hirota, S, Yamaguchi, H, Hori, H, Kuroda, S, Tanizawa, K

Biochemistry　Vol. 43　No. 8　2004/03/02

Identification of a tissue-non-specific homologue of axonal fasciculation and elongation protein zeta-1.

Fujita T, Ikuta J, Hamada J, Okajima T, Tatematsu K, Tanizawa K, Kuroda S

Biochemical and biophysical research communications　Vol. 313　No. 3　p. 738-744　2004/01　Research paper (scientific journal)

Molecular Function of Axonal Guidance-related Proteins, UNC-76/FEZ1/FEZ2

Recent Res. Devel. Biophys. Biochem.　Vol. 4　p. 313-320　2004

Stereochemistry of 2-phenylethylamine oxidation catalyzed by bacterial copper amine oxidase

M Uchida, A Ohtani, N Kohyama, T Okajima, K Tanizawa, Y Yamamoto

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY　Vol. 67　No. 12　p. 2664-2667　2003/12　Research paper (scientific journal)

Detection of protein–protein interactions on SiO2/Si surfaces by spectroscopic ellipsometry

Seigo Kodera, Toshihide Okajima, Hidehiko Iwabuki, Daisuke Kitaguchi, Shun’ichi Kuroda, Tatsuo Yoshinobu, Katsuyuki Tanizawa, Masamitsu Futai, Hiroshi Iwasaki

Analytical Biochemistry　Vol. 321　No. 1　p. 65-70　2003/10　Research paper (scientific journal)

Chemical and Kinetic Reaction Mechanisms of Quinohemoprotein Amine Dehydrogenase from Paracoccus denitrificans

Dapeng Sun, Kazutoshi Ono, Toshihide Okajima, Katsuyuki Tanizawa, Mayumi Uchida, Yukio Yamamoto, F. Scott Mathews, Victor L. Davidson

Biochemistry　Vol. 42　No. 37　p. 10896-10903　2003/09　Research paper (scientific journal)

Novel trimeric adenylate kinase from an extremely thermoacidophilic archaeon, Sulfolobus solfataricus: molecular cloning, nucleotide sequencing, expression in Escherichia coli, and characterization of the recombinant enzyme.

Toshihide Okajima, Daisuke Kitaguchi, Kumiko Fujii, Hidetada Matsuoka, Sachio Goto, Susumu Uchiyama, Yuji Kobayashi, Katsuyuki Tanizawa

Bioscience, biotechnology, and biochemistry　Vol. 66　No. 10　p. 2112-24　2002/10

HepG2細胞表面レセプター特異的抗体の機能的スクリーニング

北口 大輔, 岩蕗 秀彦, 山田 忠範, 立松 健司, 黒田 俊一, 岡島 俊英, 谷澤 克行

生化学　Vol. 74　No. 8　p. 946-946　2002/08

無血清培養により大量発現させた新規骨形成因子NELL1の生化学的解析

朴井 伸行, Ting Kang, 金山 範洋, 岩蕗 秀彦, 立松 健司, 岡島 俊英, 谷澤 克行, 黒田 俊一

生化学　Vol. 74　No. 8　p. 804-804　2002/08

RING-fingerを有する新規転写因子RPY1の低浸透圧下におけるMpk1との相互作用

八尾 修平, 小柳 智義, 立松 健司, 金子 嘉信, 原島 俊, 岡島 俊英, 谷澤 克行, 黒田 俊一

生化学　Vol. 74　No. 8　p. 926-926　2002/08

Scanning tunnelling microscopy images of the copper-containing amine oxidase from Arthrobacter globiformis in the holo and apo forms adsorbed on gold under ambient conditions

SA Contera, T Okajima, H Iwasaki

JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS SHORT NOTES & REVIEW PAPERS　Vol. 41　No. 6A　p. 3916-3921　2002/06　Research paper (scientific journal)

3H1000 Time resolved X-ray structure analysis of topaquinone biogenesis in copper-containing amine oxidase

Yamaguchi H., Kim M., Okajima T., Yoshimura M., Kishishita S., Tanizawa K.

Seibutsu Butsuri　Vol. 42　No. 2　2002

Spectroscopic observation of intermediates formed during the oxidative half-reaction of copper/topa quinone-containing phenylethylamine oxidase

S Hirota, T Iwamoto, S Kishishita, T Okajima, O Yamauchi, K Tanizawa

BIOCHEMISTRY　Vol. 40　No. 51　p. 15789-15796　2001/12　Research paper (scientific journal)

The covalent structure of the small subunit from Pseudomonas putida amine dehydrogenase reveals the presence of three novel types of internal cross-linkages, all involving cysteine in a thioether bond

Vandenberghe, I, JK Kim, B Devreese, A Hacisalihoglu, H Iwabuki, T Okajima, S Kuroda, O Adachi, JA Jongejan, JA Duine, K Tanizawa, J Van Beeumen

JOURNAL OF BIOLOGICAL CHEMISTRY　Vol. 276　No. 46　p. 42923-42931　2001/11　Research paper (scientific journal)

心筋・骨格筋特異的PDZ-LIMタンパク質ENH1と相互作用するタンパク質の同定

岩田 美紀, 松村 清之, 中川 宣孝, 立松 健司, 岡島 俊英, 谷澤 克行, 黒田 俊一

生化学　Vol. 73　No. 8　p. 866-866　2001/08

Thermal unfolding of chitosanase from Streptomyces sp. N174: role of tryptophan residues in the protein structure stabilization

Yuji Honda, Tamo Fukamizo, Toshihide Okajima, Sachio Goto, Isabelle Boucher, Ryszard Brzezinski

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology　Vol. 1429　No. 2　p. 365-376　1999/01　Research paper (scientific journal)

Exchange of Nucleoside Monophosphate-Binding Domains in Adenylate Kinase and UMP/CMP Kinase

T. Okajima, T. Fukamizo, S. Goto, T. Fukui, K. Tanizawa

Journal of Biochemistry　Vol. 124　No. 2　p. 359-367　1998/08/01　Research paper (scientific journal)

高度好熱菌由来アデニル酸キナーゼの初期的な構造研究

渡辺 充, 北口 大輔, 椋本 薫, 後藤 幸男, 岡島 俊英, 山口 宏

日本結晶学会誌　Vol. 40　p. 109-109　1998

Cloning, Sequencing, and Expression in Escherichia coli of cDNA Encoding Porcine Brain UMP-CMP Kinase1

Toshihide Okajima, Sachio Goto, Katsuyuki Tanizawa, Mitsuo Tagaya, Toshio Fukui, Hiroshi Shimofuruya, Jiro Suzuki

The Journal of Biochemistry　Vol. 117　No. 5　p. 980-986　1995/05　Research paper (scientific journal)

Site-directed mutagenesis of AMP-binding residues in adenylate kinase Alteration of substrate specificity

Toshihide Okajima, Katsuyuki Tanizawa, Toshio Fukui

FEBS Letters　Vol. 334　No. 1　p. 86-88　1993/11/08　Research paper (scientific journal)

Site-Directed Random Mutagenesis of AMP-Binding Residues in Adenylate Kinase1

Toshihide Okajima, Katsuyuki Tanizawa, Toshio Fukui

The Journal of Biochemistry　Vol. 114　No. 5　p. 627-633　1993/11　Research paper (scientific journal)

Role of Leucine 66 in the Asymmetric Recognition of Substrates in the Chicken Muscle Adenylate Kinase

T. Okajima, K. Tanizawa, T. Yoneya, T. Fukui

J. Biol. Chem.　Vol. 266　No. 18　p. 11442-11447　1991/06　Research paper (scientific journal)

The Role of Leu-190 in the Function and Stability of Adenylate Kinase

T. Yoneya, T. Okajima, M. Tagaya, K. Tanizawa, T. Fukui

J. Biol. Chem.　Vol. 265　No. 35　p. 21488-21493　1990/12　Research paper (scientific journal)

Chemical modification of tryptophan residues and stability changes in proteins

Toshihide Okajima, Yasushi Kawata, Kozo Hamaguchi

Biochemistry　Vol. 29　No. 39　p. 9168-9175　1990/10　Research paper (scientific journal)