Catecholaminergic cell type-specific expression of Cre recombinase in knock-in transgenic rats generated by the Combi-CRISPR technology

Natsuki Matsushita, Kayo Nishizawa, Shigeki Kato, Yoshio Iguchi, Ryoji Fukabori, Kosei Takeuchi, Yoshiki Miyasaka, Tomoji Mashimo, Kazuto Kobayashi

Journal of Neuroscience Methods　Vol. 381　p. 109707-109707　2022/11　Research paper (scientific journal)

CRISPR-mediated Bmpr2 point mutation exacerbates late pulmonary vasculopathy and reduces survival in rats with experimental pulmonary hypertension.

Jane Chanda Kabwe, Hirofumi Sawada, Yoshihide Mitani, Hironori Oshita, Naoki Tsuboya, Erquan Zhang, Junko Maruyama, Yoshiki Miyasaka, Hideyoshi Ko, Kazunobu Oya, Hiromasa Ito, Noriko Yodoya, Shoichiro Otsuki, Hiroyuki Ohashi, Ryuji Okamoto, Kaoru Dohi, Yuhei Nishimura, Tomoji Mashimo, Masahiro Hirayama, Kazuo Maruyama

Respiratory research　Vol. 23　No. 1　p. 87-87　2022/04/08　Research paper (scientific journal)

Impact of GAD65 and/or GAD67 deficiency on perinatal development in rats.

Weiru Jiang, Toshikazu Kakizaki, Kazuyuki Fujihara, Shigeo Miyata, Yue Zhang, Takashi Suto, Daiki Kato, Shigeru Saito, Koji Shibasaki, Yasuki Ishizaki, Koji Isoda, Hideaki Yokoo, Hideru Obinata, Touko Hirano, Yoshiki Miyasaka, Tomoji Mashimo, Yuchio Yanagawa

FASEB journal : official publication of the Federation of American Societies for Experimental Biology　Vol. 36　No. 2　2022/02　Research paper (scientific journal)

A high-quality severe combined immunodeficiency (SCID) rat bioresource.

Yoshiki Miyasaka, Jinxi Wang, Kosuke Hattori, Yuko Yamauchi, Miho Hoshi, Kazuto Yoshimi, Saeko Ishida, Tomoji Mashimo

PloS one　Vol. 17　No. 8　2022　Research paper (scientific journal)

Aberrant accumulation of TMEM43 accompanied by perturbed transmural gene expression in arrhythmogenic cardiomyopathy.

Haruki Shinomiya, Hisakazu Kato, Yuki Kuramoto, Nozomi Watanabe, Toshihiro Tsuruda, Tadaaki Arimura, Yohei Miyashita, Yoshiki Miyasaka, Tomoji Mashimo, Ayako Takuwa, Daisuke Motooka, Daisuke Okuzaki, Ken Matsuoka, Osamu Tsukamoto, Hideyuki Hakui, Noriaki Yamada, Jong-Kook Lee, Hidetaka Kioka, Masafumi Kitakaze, Seiji Takashima, Yasushi Sakata, Yoshihiro Asano

FASEB journal : official publication of the Federation of American Societies for Experimental Biology　Vol. 35　No. 11　2021/11　Research paper (scientific journal)

A humanised rat model reveals ultrastructural differences between bone and mineralised tumour tissue.

Christoph A Lahr, Marietta Landgraf, Ferdinand Wagner, Amaia Cipitria, Inés Moreno-Jiménez, Onur Bas, Beat Schmutz, Christoph Meinert, Tomoji Mashimo, Yoshiki Miyasaka, Boris M Holzapfel, Abbas Shafiee, Jacqui A McGovern, Dietmar W Hutmacher

Bone　Vol. 158　p. 116018-116018　2021/05/20　Research paper (scientific journal)

Response to correspondence on "Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation".

Channabasavaiah B Gurumurthy, Aidan R O'Brien, Rolen M Quadros, John Adams Jr, Pilar Alcaide, Shinya Ayabe, Johnathan Ballard, Surinder K Batra, Marie-Claude Beauchamp, Kathleen A Becker, Guillaume Bernas, David Brough, Francisco Carrillo-Salinas, Wesley Chan, Hanying Chen, Ruby Dawson, Victoria DeMambro, Jinke D'Hont, Katharine Dibb, James D Eudy, Lin Gan, Jing Gao, Amy Gonzales, Anyonya Guntur, Huiping Guo, Donald W Harms, Anne Harrington, Kathryn E Hentges, Neil Humphreys, Shiho Imai, Hideshi Ishii, Mizuho Iwama, Eric Jonasch, Michelle Karolak, Bernard Keavney, Nay-Chi Khin, Masamitsu Konno, Yuko Kotani, Yayoi Kunihiro, Imayavaramban Lakshmanan, Catherine Larochelle, Catherine B Lawrence, Lin Li, Volkhard Lindner, Xian-De Liu, Gloria Lopez-Castejon, Andrew Loudon, Jenna Lowe, Loydie Jerome-Majeweska, Taiji Matsusaka, Hiromi Miura, Yoshiki Miyasaka, Benjamin Morpurgo, Katherine Motyl, Yo-Ichi Nabeshima, Koji Nakade, Toshiaki Nakashiba, Kenichi Nakashima, Yuichi Obata, Sanae Ogiwara, Mariette Ouellet, Leif Oxburgh, Sandra Piltz, Ilka Pinz, Moorthy P Ponnusamy, David Ray, Ronald J Redder, Clifford J Rosen, Nikki Ross, Mark T Ruhe, Larisa Ryzhova, Ane M Salvador, Sabrina Shameen Alam, Radislav Sedlacek, Karan Sharma, Chad Smith, Katrien Staes, Lora Starrs, Fumihiro Sugiyama, Satoru Takahashi, Tomohiro Tanaka, Andrew Trafford, Yoshihiro Uno, Leen Vanhoutte, Frederique Vanrockeghem, Brandon J Willis, Christian S Wright, Yuko Yamauchi, Xin Yi, Kazuto Yoshimi, Xuesong Zhang, Yu Zhang, Masato Ohtsuka, Satyabrata Das, Daniel J Garry, Tino Hochepied, Paul Thomas, Jan Parker-Thornburg, Antony D Adamson, Atsushi Yoshiki, Jean-Francois Schmouth, Andrei Golovko, William R Thompson, K C Kent Lloyd, Joshua A Wood, Mitra Cowan, Tomoji Mashimo, Seiya Mizuno, Hao Zhu, Petr Kasparek, Lucy Liaw, Joseph M Miano, Gaetan Burgio

Genome biology　Vol. 22　No. 1　p. 99-99　2021/04/07　Research paper (scientific journal)

Genetic deletion of the 67-kDa isoform of glutamate decarboxylase alters conditioned fear behavior in rats.

Kazuyuki Fujihara, Takumi Sato, Yoshiki Miyasaka, Tomoji Mashimo, Yuchio Yanagawa

FEBS open bio　Vol. 11　No. 2　p. 340-353　2021/02　Research paper (scientific journal)

Combi-CRISPR: combination of NHEJ and HDR provides efficient and precise plasmid-based knock-ins in mice and rats.

Kazuto Yoshimi, Yuichiro Oka, Yoshiki Miyasaka, Yuko Kotani, Misato Yasumura, Yoshihiro Uno, Kosuke Hattori, Arisa Tanigawa, Makoto Sato, Manami Oya, Kazuhiro Nakamura, Natsuki Matsushita, Kazuto Kobayashi, Tomoji Mashimo

Human genetics　Vol. 140　No. 2　p. 277-287　2021/02　Research paper (scientific journal)

Behavioral Consequences of a Combination of Gad1 Haplodeficiency and Adolescent Exposure to an NMDA Receptor Antagonist in Long-Evans Rats.

Kazuyuki Fujihara, Takumi Sato, Kazuya Higeta, Yoshiki Miyasaka, Tomoji Mashimo, Yuchio Yanagawa

Frontiers in pharmacology　Vol. 12　p. 646088-646088　2021　Research paper (scientific journal)

CRISPR/Cas9-engineered Gad1 elimination in rats leads to complex behavioral changes: implications for schizophrenia.

Kazuyuki Fujihara, Kazuo Yamada, Yukio Ichitani, Toshikazu Kakizaki, Weiru Jiang, Shigeo Miyata, Takashi Suto, Daiki Kato, Shigeru Saito, Masahiko Watanabe, Yuki Kajita, Tomokazu Ohshiro, Hajime Mushiake, Yoshiki Miyasaka, Tomoji Mashimo, Hiroki Yasuda, Yuchio Yanagawa

Translational psychiatry　Vol. 10　No. 1　p. 426-426　2020/12/08　Research paper (scientific journal)

Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation.

Channabasavaiah B Gurumurthy, Aidan R O'Brien, Rolen M Quadros, John Adams Jr, Pilar Alcaide, Shinya Ayabe, Johnathan Ballard, Surinder K Batra, Marie-Claude Beauchamp, Kathleen A Becker, Guillaume Bernas, David Brough, Francisco Carrillo-Salinas, Wesley Chan, Hanying Chen, Ruby Dawson, Victoria DeMambro, Jinke D'Hont, Katharine M Dibb, James D Eudy, Lin Gan, Jing Gao, Amy Gonzales, Anyonya R Guntur, Huiping Guo, Donald W Harms, Anne Harrington, Kathryn E Hentges, Neil Humphreys, Shiho Imai, Hideshi Ishii, Mizuho Iwama, Eric Jonasch, Michelle Karolak, Bernard Keavney, Nay-Chi Khin, Masamitsu Konno, Yuko Kotani, Yayoi Kunihiro, Imayavaramban Lakshmanan, Catherine Larochelle, Catherine B Lawrence, Lin Li, Volkhard Lindner, Xian-De Liu, Gloria Lopez-Castejon, Andrew Loudon, Jenna Lowe, Loydie A Jerome-Majewska, Taiji Matsusaka, Hiromi Miura, Yoshiki Miyasaka, Benjamin Morpurgo, Katherine Motyl, Yo-Ichi Nabeshima, Koji Nakade, Toshiaki Nakashiba, Kenichi Nakashima, Yuichi Obata, Sanae Ogiwara, Mariette Ouellet, Leif Oxburgh, Sandra Piltz, Ilka Pinz, Moorthy P Ponnusamy, David Ray, Ronald J Redder, Clifford J Rosen, Nikki Ross, Mark T Ruhe, Larisa Ryzhova, Ane M Salvador, Sabrina Shameen Alam, Radislav Sedlacek, Karan Sharma, Chad Smith, Katrien Staes, Lora Starrs, Fumihiro Sugiyama, Satoru Takahashi, Tomohiro Tanaka, Andrew W Trafford, Yoshihiro Uno, Leen Vanhoutte, Frederique Vanrockeghem, Brandon J Willis, Christian S Wright, Yuko Yamauchi, Xin Yi, Kazuto Yoshimi, Xuesong Zhang, Yu Zhang, Masato Ohtsuka, Satyabrata Das, Daniel J Garry, Tino Hochepied, Paul Thomas, Jan Parker-Thornburg, Antony D Adamson, Atsushi Yoshiki, Jean-Francois Schmouth, Andrei Golovko, William R Thompson, K C Kent Lloyd, Joshua A Wood, Mitra Cowan, Tomoji Mashimo, Seiya Mizuno, Hao Zhu, Petr Kasparek, Lucy Liaw, Joseph M Miano, Gaetan Burgio

Genome biology　Vol. 20　No. 1　p. 171-171　2019/08/26　Research paper (scientific journal)

Null mutation of the endothelin receptor type B gene causes embryonic death in the GK rat

Jinxi Wang, Ruihua Dang, Yoshiki Miyasaka, Kousuke Hattori, Daisuke Torigoe, Tadashi Okamura, Hassan T. Tag-EI-Din-Hassan, Masami Morimatsu, Tomoji Mashimo, Takashi Agui

PLOS ONE　Vol. 14　No. 6　p. e0217132-e0217132　2019/06/06　Research paper (scientific journal)

CLICK: one-step generation of conditional knockout mice

Yoshiki Miyasaka, Yoshihiro Uno, Kazuto Yoshimi, Yayoi Kunihiro, Takuji Yoshimura, Tomohiro Tanaka, Harumi Ishikubo, Yuichi Hiraoka, Norihiko Takemoto, Takao Tanaka, Yoshihiro Ooguchi, Paul Skehel, Tomomi Aida, Junji Takeda, Tomoji Mashimo

BMC Genomics　Vol. 19　No. 1　p. 318-318　2018/12　Research paper (scientific journal)

Re-Evaluating One-step Generation of Mice Carrying Conditional Alleles by CRISPR-Cas9-Mediated Genome Editing Technology

Channabasavaiah Gurumurthy, Rolen Quadros, John Adams, Pilar Alcaide, Shinya Ayabe, Johnathan Ballard, Surinder K. Batra, Marie-Claude Beauchamp, Kathleen A Becker, Guillaume Bernas, David Brough, Francisco Carrillo-Salinas, Ruby Dawson, Victoria DeMambro, Jinke D’Hont, Katharine Dibb, James D. Eudy, Lin Gan, Jing Gao, Amy Gonzales, Anyonya Guntur, Huiping Guo, Donald W. Harms, Anne Harrington, Kathryn E. Hentges, Neil Humphreys, Shiho Imai, Hideshi Ishii, Mizuho Iwama, Eric Jonasch, Michelle Karolak, Bernard Keavney, Nay-Chi Khin, Masamitsu Konno, Yuko Kotani, Yayoi Kunihiro, Imayavaramban Lakshmanan, Catherine Larochelle, Catherine B. Lawrence, Lin Li, Volkhard Lindner, Xian-De Liu, Gloria Lopez-Castejon, Andrew Loudon, Jenna Lowe, Loydie Jerome-Majeweska, Taiji Matsusaka, Hiromi Miura, Yoshiki Miyasaka, Benjamin Morpurgo, Katherine Motyl, Yo-ichi Nabeshima, Koji Nakade, Toshiaki Nakashiba, Kenichi Nakashima, Yuichi Obata, Sanae Ogiwara, Mariette Ouellet, Leif Oxburgh, Sandra Piltz, Ilka Pinz, Moorthy P. Ponnusamy, David Ray, Ronald J. Redder, Clifford J Rosen, Nikki Ross, Mark T. Ruhe, Larisa Ryzhova, Ane M. Salvador, Radislav Sedlacek, Karan Sharma, Chad Smith, Katrien Staes, Lora Starrs, Fumihiro Sugiyama, Satoru Takahashi, Tomohiro Tanaka, Andrew Trafford, Yoshihiro Uno, Leen Vanhoutte, Frederique Vanrockeghem, Brandon J. Willis, Christian S. Wright, Yuko Yamauchi, Xin Yi, Kazuto Yoshimi, Xuesong Zhang, Yu Zhang, Masato Ohtsuka, Satyabrata Das, Daniel J. Garry, Tino Hochepied, Paul Thomas, Jan Parker-Thornburg, Antony D Adamson, Atsushi Yoshiki, Jean-Francois Schmouth, Andrei Golovko, William R. Thompson, KC. Kent Lloyd, Joshua A. Wood, Mitra Cowan, Tomoji Mashimo, Seiya Mizuno, Hao Zhu, Petr Kasparek, Lucy Liaw, Joseph M. Miano, Gaetan Burgio

2018/08/30

ゲノム編集概説 基礎技術として利用されるCRISPR/Cas9

宮坂 佳樹, 吉見 一人, 真下 知士

アンチ・エイジング医学　Vol. 14　No. 4　p. 506-512　2018/08

ゲノム編集が広げるラットリソースの可能性 NBRP-Ratと先端モデル動物支援

宮坂 佳樹, 吉見 一人, 國廣 弥生, 服部 晃佑, 山内 祐子, 小谷 祐子, 清水 加奈子, 宮川 真智子, 水野 洋子, 夘野 善弘, 真下 知士

生命科学系学会合同年次大会　Vol. 2017年度　p. [2P-1361]　2017/12