顔写真

PHOTO

Kai Toshie
甲斐　歳惠
Kai Toshie
甲斐　歳惠
Institutional repository
Graduate School of Frontier Biosciences　Department of Frontier Biosciences, Professor
Institutional repository

Research History 10

  1. 2025/04 - Present
    The University of Osaka

  2. 2015/10 - Present
    Osaka University Graduate School of Frontier Biosciences　Professor

  3. 2021/04 - 2024/03
    Osaka University　Center for Global Initiatives　Director of Student Mobility Unit

  4. 2011/01 - 2015/09
    The National University of Singapore　Department of Biological Science　Adjunct Associate Professor

  5. 2010/10 - 2015/09
    Temasek Lifesciences Laboratory　Senior Principal Investigator

  6. 2007/02 - 2010/12
    The National University of Singapore　Department of Biological Science　Adjunct Assistant Professor

  7. 2005/06 - 2010/09
    Temasek Lifesciences Laboratory　Principal Investigator

  8. 2001/01 - 2005/06
    Carnegie Institution of Washington/Howard Hughes Medical Institute　Department of Embryology　Research Associate

  9. 2000/01 - 2001/12
    Science and Technology Agency/Carnegie Institution of Washington　Department of Embryology　Long-term overseas researcher

  10. 1999/10 - 1999/12
    Carnegie Institution of Washington/Howard Hughes Medical Institute　Department of Embryology　Research Associate

Education 1

  1. Osaka University　Graduate School of Science

    1993/04 - 1998/03

Committee Memberships 4

  1. 日本分子生物学会　キャリアパス委員

    2023/04 - Present

  2. Osaka University　Senior Advisor to the President

    2021/04 - Present

  3. 大阪科学賞 選考委員会　選考委員

    2021 - Present

  4. 第45回日本分子生物学会組織委員会　プログラム委員長

    2020 - 2022/12

Professional Memberships 3

  1. 日本発生生物学会

  2. 日本分子生物学会

  3. 日本RNA学会

Research Areas 2

  1. Life sciences / Developmental biology /

  2. Life sciences / Molecular biology /

Awards 1

  1. 大阪大学賞

    大阪大学　2021

Papers 46

  1. Paralog‐Dependent Specialization of Paf1C Subunit, Ctr9, for Sex Chromosome Gene Regulation and Male Germline Differentiation in Drosophila

    Toshie Kai, Jinglan Zheng, Taichiro Iki

    Genes to Cells　2025/09　Research paper (scientific journal)

  2. piRNA processing within non‐membrane structures is governed by constituent proteins and their functional motifs

    Ritsuko Suyama, Toshie Kai

    The FEBS Journal　2025/06　Research paper (scientific journal)

  3. Transcriptional Repression of reaper by Stand Still Safeguards Female Germline Development in Drosophila

    Masaya Matsui, Shinichi Kawaguchi, Toshie Kai

    2025/05/21

  4. Factories without walls: The molecular architecture and functions of non-membrane organelles in small RNA-guided genome protection.

    Shinichi Kawaguchi, Wakana Isshiki, Toshie Kai

    Biochimica et biophysica acta. General subjects　Vol. 1869　No. 7　p. 130811-130811　2025/05/03　Research paper (scientific journal)

  5. In silico screening by AlphaFold2 program revealed the potential binding partners of nuage-localizing proteins and piRNA-related proteins

    Shinichi Kawaguchi, Xu Xin, Soga Takashi, Yamaguchi Kenta, Kawasaki Ryuuya, Shimouchi Ryota, Date Susumu, Kai Toshie

    eLife　2025/04/22

    Publisher: eLife Sciences Publications, Ltd

  6. HemK2 functions for sufficient protein synthesis and RNA stability through eRF1 methylation during Drosophila oogenesis.

    Fengmei Xu, Ritsuko Suyama, Toshifumi Inada, Shinichi Kawaguchi, Toshie Kai

    Development (Cambridge, England)　Vol. 151　No. 14　2024/07/15　Research paper (scientific journal)

    Publisher: Cold Spring Harbor Laboratory

  7. Microbes control Drosophila germline stem cell increase and egg maturation through hormonal pathways

    Ritsuko Suyama, Nicolas Cetraro, Joanne Y. Yew, Toshie Kai

    Communications Biology　2023/12/20　Research paper (scientific journal)

  8. Tejas functions as a core component in nuage assembly and precursor processing in Drosophila piRNA biogenesis.

    Yuxuan Lin, Ritsuko Suyama, Shinichi Kawaguchi, Taichiro Iki, Toshie Kai

    The Journal of cell biology　Vol. 222　No. 10　2023/10/02　Research paper (scientific journal)

  9. miRNA/siRNA-directed pathway to produce noncoding piRNAs from endogenous protein-coding regions ensures Drosophila spermatogenesis.

    Taichiro Iki, Shinichi Kawaguchi, Toshie Kai

    Science advances　Vol. 9　No. 29　2023/07/21　Research paper (scientific journal)

  10. Nondomain biopolymers: Flexible molecular strategies to acquire biological functions.

    Kazuharu Arakawa, Tetsuro Hirose, Toshifumi Inada, Takuhiro Ito, Toshie Kai, Masaaki Oyama, Yukihide Tomari, Takao Yoda, Shinichi Nakagawa

    Genes to cells : devoted to molecular & cellular mechanisms　2023/05/30　Research paper (scientific journal)

  11. Post-Covid International Exchange Through Lateral Cooperation : Osaka University Virtual University Tour as New Practices of Online International Student Recruitment

    Osaka University Higher Education Studies　2023/03　Research paper (bulletin of university, research institution)

  12. The Tudor Domain-Containing Protein, Kotsubu (CG9925), Localizes to the Nuage and Functions in piRNA Biogenesis in D. melanogaster.

    Lin-Xenia Lim, Wakana Isshiki, Taichiro Iki, Shinichi Kawaguchi, Toshie Kai

    Frontiers in molecular biosciences　Vol. 9　p. 818302-818302　2022　Research paper (scientific journal)

  13. Drosophila MARF1 ensures proper oocyte maturation by regulating nanos expression

    Shinichi Kawaguchi, Mizuki Ueki, Toshie Kai

    PLOS ONE　Vol. 15　No. 4　p. e0231114-e0231114　2020/04/03　Research paper (scientific journal)

    Publisher: Public Library of Science (PLoS)

  14. Editorial overview: Stem cells orchestrate oogenesis: a lesson from the fruit fly, Drosophila melanogaster

    Ryusuke Niwa, Toshie Kai

    Current Opinion in Insect Science　2020/02　Research paper (scientific journal)

  15. Modulation of Ago2 Loading by Cyclophilin 40 Endows a Unique Repertoire of Functional miRNAs During Sperm Maturation in Drosophila

    Taichiro Iki, Moe Takami, Toshie Kai

    Cell Reports　2020　Research paper (scientific journal)

    Publisher: Elsevier BV

  16. Protecting and diversifying the germline

    Ryan J. Gleason, Amit Anand, Toshie Kai, Xin Chen

    Genetics　Vol. 208　No. 2　p. 435-471　2018/02/01　Research paper (scientific journal)

    Publisher: Genetics Society of America

  17. Heterochromatin protein 1a functions for piRNA biogenesis predominantly from pericentric and telomeric regions in Drosophila

    Teo, Ryan Yee Wei, An, , Amit, Sridhar, Vishweshwaren, Okamura, Katsutomo, Kai, Toshie

    Nature communications　Vol. 9　No. 1　p. 1735-1735　2018　Research paper (scientific journal)

    Publisher: Nature Publishing Group

  18. The piRNA pathway is developmentally regulated during spermatogenesis in Drosophila

    Emilie Quenerch'du, Amit Anand, Toshie Kai

    RNA　Vol. 22　No. 7　p. 1044-1054　2016/07　Research paper (scientific journal)

  19. Germline Cells Drive lives : Beyond Birth and Death of Individuals

    Vol. 68　No. 2　p. 101-104　2016/04

    Publisher:

  20. A piece of the pi(e): The diverse roles of animal piRNAs and their PIWI partners

    Robyn S. M. Lim, Toshie Kai

    SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY　Vol. 47-48　p. 17-31　2015/12

  21. The Tudor domain protein Tapas, a homolog of the vertebrate Tdrd7, functions in the piRNA pathway to regulate retrotransposons in germline of Drosophila melanogaster

    Veena S. Patil, Amit Anand, Alisha Chakrabarti, Toshie Kai

    BMC BIOLOGY　Vol. 12　No. 1　p. 61-75　2014/10　Research paper (scientific journal)

  22. Response to Zhang et al

    Toshie Kai

    The EMBO Journal　2014/03/18　Research paper (scientific journal)

  23. Analysis of Hydra PIWI proteins and piRNAs uncover early evolutionary origins of the piRNA pathway

    Robyn S. M. Lim, Amit Anand, Chiemi Nishimiya-Fujisawa, Satoru Kobayashi, Toshie Kai

    DEVELOPMENTAL BIOLOGY　Vol. 386　No. 1　p. 237-251　2014/02　Research paper (scientific journal)

  24. Polo-mediated phosphorylation of Maelstrom regulates oocyte determination during oogenesis in Drosophila

    Jun Wei Pek, Bing Fu Ng, Toshie Kai

    DEVELOPMENT　Vol. 139　No. 24　p. 4505-4513　2012/12　Research paper (scientific journal)

  25. Isolation of Undifferentiated Female Germline Cells from Adult Drosophila Ovaries.

    Lim RS, Osato M, Kai T

    Curr. Protoc. Stem Cell Biol.　Vol. 34　p. 2E.3.1-2E.3.15　2012/08

  26. Tudor domain proteins in development

    Jun Wei Pek, Amit Anand, Toshie Kai

    DEVELOPMENT　Vol. 139　No. 13　p. 2255-2266　2012/07　Research paper (scientific journal)

  27. The tudor domain protein Kumo is required to assemble the nuage and to generate germline piRNAs in Drosophila

    Amit Anand, Toshie Kai

    EMBO JOURNAL　Vol. 31　No. 4　p. 870-882　2012/02　Research paper (scientific journal)

  28. piRNA pathway and the potential processing site, the nuage, in the Drosophila germline

    Jun Wei Pek, Veena S. Patil, Toshie Kai

    DEVELOPMENT GROWTH & DIFFERENTIATION　Vol. 54　No. 1　p. 66-77　2012/01

  29. DEAD-box RNA helicase Belle/DDX3 and the RNA interference pathway promote mitotic chromosome segregation

    Jun Wei Pek, Toshie Kai

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　Vol. 108　No. 29　p. 12007-12012　2011/07　Research paper (scientific journal)

  30. Non-coding RNAs enter mitosis: functions, conservation and implications

    Jun Wei Pek, Toshie Kai

    CELL DIVISION　Vol. 6　No. 1　p. 6-11　2011/02　Research paper (scientific journal)

  31. A Role for Vasa in Regulating Mitotic Chromosome Condensation in Drosophila

    Jun Wei Pek, Toshie Kai

    CURRENT BIOLOGY　Vol. 21　No. 1　p. 39-44　2011/01　Research paper (scientific journal)

  32. Repression of Retroelements in Drosophila Germline via piRNA Pathway by the Tudor Domain Protein Tejas

    Veena S. Patil, Toshie Kai

    CURRENT BIOLOGY　Vol. 20　No. 8　p. 724-730　2010/04　Research paper (scientific journal)

  33. Drosophila Maelstrom Ensures Proper Germline Stem Cell Lineage Differentiation by Repressing microRNA-7

    Jun Wei Pek, Ai Khim Lim, Toshie Kai

    DEVELOPMENTAL CELL　Vol. 17　No. 3　p. 417-424　2009/09　Research paper (scientific journal)

  34. piRNAs mediate posttranscriptional retroelement silencing and localization to pi-bodies in the Drosophila germline

    Ai Khim Lim, Liheng Tao, Toshie Kai

    JOURNAL OF CELL BIOLOGY　Vol. 186　No. 3　p. 333-342　2009/08　Research paper (scientific journal)

  35. Conserved germline organelle, nuage, serves as site for processing of Piwi-interacting RNAs.

    Pek JW, Kai T

    Experimental Medicine　Vol. 27　No. 3　p. 393-399　2009/01

  36. Unique germ-line organelle, nuage, functions to repress selfish genetic elements in Drosophila melanogaster

    Ai Khim Lim, Toshie Kai

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　Vol. 104　No. 16　p. 6714-6719　2007/04　Research paper (scientific journal)

  37. The expression profile of purified Drosophila germline stem cells

    T Kai, D Williams, AC Spradling

    DEVELOPMENTAL BIOLOGY　Vol. 283　No. 2　p. 486-502　2005/07　Research paper (scientific journal)

  38. Germline stem cells and their niches.

    Kai T

    Tanpakushitsu Kakusan Koso　Vol. 49　No. 6　p. 710-717　2004/05　Research paper (scientific journal)

  39. Differentiating germ cells can revert into functional stem cells in Drosophila melanogaster ovaries

    T Kai, A Spradling

    NATURE　Vol. 428　No. 6982　p. 564-569　2004/04　Research paper (scientific journal)

  40. An empty Drosophila stem cell niche reactivates the proliferation of ectopic cells

    TS Kai, A Spradling

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　Vol. 100　No. 8　p. 4633-4638　2003/04　Research paper (scientific journal)

  41. Multiple mechanisms for degradation of bacteriophage T4 soc mRNA.

    Toshie Kai, Tetsuro Yonesaki

    Genetics　Vol. 160　No. 1　p. 5-12　2002/01　Research paper (scientific journal)

  42. Stem cells find their niche

    Allan Spradling, Daniela Drummond-Barbosa, Toshie Kai

    Nature　Vol. 414　No. 6859　p. 98-104　2001/11

  43. [Discrimination and specific degradation of mRNAs: a new turn with T4 phage gene silencing].

    H Ueno, T Yonesaki, T Kai

    Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme　Vol. 44　No. 13　p. 1959-66　1999/10　Research paper (scientific journal)

  44. Gene 61.3 of bacteriophage T4 is the spackle gene.

    T Kai, H Ueno, Y Otsuka, W Morimoto, T Yonesaki

    Virology　Vol. 260　No. 2　p. 254-9　1999/08/01　Research paper (scientific journal)

  45. Involvement of other bacteriophage T4 genes in the blockade of protein synthesis and mRNA destabilization by a mutation of gene 61. 5.

    T Kai, H Ueno, T Yonesaki

    Virology　Vol. 248　No. 1　p. 148-55　1998/08/15　Research paper (scientific journal)

  46. Destabilization of bacteriophage T4 mRNAs by a mutation of gene 61.5.

    T Kai, H E Selick, T Yonesaki

    Genetics　Vol. 144　No. 1　p. 7-14　1996/09　Research paper (scientific journal)

Misc. 4

  1. Non-membranous RNP condensates control the fate of germline cells

    須山律子, 甲斐歳惠

    実験医学　Vol. 39　No. 10　2021

  2. HP1a functions to suppress the evolutionarily fixed and telomeric transposons via piRNAs

    Amit Anand, Teo Yee Wei, Katsutomo Okamura, Toshie Kai

    GENES & GENETIC SYSTEMS　Vol. 91　No. 6　p. 331-331　2016/12　Research paper, summary (international conference)

  3. Antisense piRNA amplification, but not piRNA production or nuage assembly, requires the Tudor-domain protein Qin Response

    Amit Anand, Toshie Kai

    EMBO JOURNAL　Vol. 33　No. 6　p. 540-541　2014/03　Rapid communication, short report, research note, etc. (scientific journal)

  4. Post-transcriptional retroelement surveillance is mediated by piRNAs in Drosophila germline

    Ai Khim Lim, Liheng Tao, Toshie Kai

    MECHANISMS OF DEVELOPMENT　Vol. 126　p. S51-S51　2009/08　Research paper, summary (international conference)

Presentations 22

  1. The Gender Landscape in Academia in Japan and Beyond

    Toshie KAI

    JSPS Symposium on Promoting Gender Equality　2025/02/27

  2. キャリアパス委員会主催ランチタイムセミナー2024

    三浦 恭子, 甲斐 歳惠, 佐田 亜衣子, 來生(道下, 江利子, 武部貴則, 胡桃坂 仁志

    第47回分子生物学会年会　2024/11/27

  3. Processing of small RNAs safeguarding the germline genome in the non-membrane structure, nuage

    Toshie KAI

    [From Innovations to Applications] 20th Anniversary of relations Osaka U- Strasbourg U　2024/10/17

  4. グローバル研究時代の人生とキャリアの調和

    甲斐歳惠

    日本生化学会 第64回 生命科学夏の学校　2024/08/30

  5. キャリアパス委員会主催ランチタイムセミナー2023

    甲斐 歳惠, 井関 祥子, 大川 恭行, 鐘巻 将人, 胡桃坂 仁志

    第46回分子生物学会年会　2023/12/06

  6. Molecular basis of nuage organization by Tejas and coordination for piRNA biogenesis in Drosophila

    Toshie KAI

    2022 EMBO Workshop on PIWI Proteins and piRNAs　2022/04/06

  7. piRNAs and PIWI proteins

    EMBO Workshop

  8. Tudor Domain Proteins and Small Interfering RNAs Protects Germline Cells

    The International Conference of the Genetics Society of Korea 2017　2017/10/27

  9. HP1a は進化的に古いトランスポゾンとテロメア領域にある トランスポゾンをpiRNA 経路によって抑制する

    日本遺伝学会第88回大会　2016/09/07

  10. Nuage and piRNAs function for the safeguard of germline genome

    Toshie Kai

    55th Annual Drosophila Research Conference　2014/03/30

  11. Nuage as a potential processing site of piRNAs in germline cells and stem cells

    Emilie Quenerch'du, Robyn S. M. Lim, Amit Anand, Toshie Kai

    2013/07/25

  12. Piwi proteins and piRNA function in Hydra stem cells

    Asia-Pacific Developmental Biology Conference (APDBC 2012)　2012/10/08

  13. Tudor Domain Proteins and Piwis Function in Nuage for piRNA Amplification in Stem Cells and Germline Cells

    Amit Anand, Lim, Robyn S. m, Toshie Kai

    ISSCR 2012　2012/06/15

  14. Nuage as a processing site of germline piRNA

    Conference on Genome Engineering　2011/06/20

  15. Nuage: A Potential Site for Processing of Germline piRNA

    Toshie Kai

    1st Asia-Pacific Drosophila Research Conference (APDRC)　2011/05/23

  16. Nuage: A Potential Site for Processing of Germline piRNA

    Toshie Kai

    Keystone Symposia RNA silencing　2011/03/23

  17. International Symposium on Epigenetic Modifications of the Genome: Mechanisms and Implications

    13 ADNAT Convention

  18. The biology of nuage: findings dropped from the clouds in germline cells

    Toshie Kai

    2009

  19. 第31回日本分子生物学会年会

    2008

  20. シンガポール国立大学DBS

    2006

  21. The symposium of Dynamics of Extracellular Environments

    The symposium of Dynamics of Extracellular Environments　2005

  22. Structure and function of a non-membrane organelle, nuage, in germline cells

    33rd Forum in DOJIN

Institutional Repository 8

Content Published in the University of Osaka Institutional Repository (OUKA)

  1. Factories without walls: The molecular architecture and functions of non-membrane organelles in small RNA-guided genome protection

    Kawaguchi Shinichi, Isshiki Wakana, Kai Toshie

    Biochimica et Biophysica Acta - General Subjects　Vol. 1869　No. 7　2025/05/03

  2. In silico screening by AlphaFold2 program revealed the potential binding partners of nuage-localizing proteins and piRNA-related proteins

    Kawaguchi Shinichi, Xu Xin, Soga Takashi, Yamaguchi Kenta, Kawasaki Ryuuya, Shimouchi Ryota, Date Susumu, Kai Toshie

    eLife　Vol. 13　2025/04/22

  3. piRNA processing within non-membrane structures is governed by constituent proteins and their functional motifs

    Suyama Ritsuko, Kai Toshie

    FEBS Journal　2024/12/30

  4. HemK2 functions for sufficient protein synthesis and RNA stability through eRF1 methylation during Drosophila oogenesis

    Xu Fengmei, Suyama Ritsuko, Inada Toshifumi, Kawaguchi Shinichi, Kai Toshie

    Development　Vol. 151　No. 14　2024/07/16

  5. Microbes control Drosophila germline stem cell increase and egg maturation through hormonal pathways

    Suyama Ritsuko, Cetraro Nicolas, Yew Joanne Y., Kai Toshie

    Communications Biology　Vol. 6　No. 1　2023/12/20

  6. miRNA/siRNA-directed pathway to produce noncoding piRNAs from endogenous protein-coding regions ensures Drosophila spermatogenesis

    Iki Taichiro, Kawaguchi Shinichi, Kai Toshie

    Science Advances　Vol. 9　No. 29　2023/07/21

  7. Post-Covid International Exchange Through Lateral Cooperation : Osaka University Virtual University Tour as New Practices of Online International Student Recruitment

    Zhang Xixi, Enkhtur Ariunaa, Li Ming, Kai Toshie, Hisada Hitoshi, Angkawidjaja Clement

    Osaka University Higher Education Studies　Vol. 11　p. 29-35　2023/03/20

  8. Bacteriophage T4 gene 61.5, a gene required for protein synthesis and stabilization of mRNA at late stages of infection

    甲斐 歳恵