Mouse GTSF1 is an essential factor for secondary piRNA biogenesis.

Takuji Yoshimura, Toshiaki Watanabe, Satomi Kuramochi-Miyagawa, Noriaki Takemoto, Yusuke Shiromoto, Akihiko Kudo, Masami Kanai-Azuma, Fumi Tashiro, Satsuki Miyazaki, Ami Katanaya, Shinichiro Chuma, Jun-Ichi Miyazaki

EMBO reports　Vol. 19　No. 4　2018/04　Research paper (scientific journal)

PNLDC1, mouse pre-piRNA Trimmer, is required for meiotic and post-meiotic male germ cell development.

Toru Nishimura, Ippei Nagamori, Tsunetoshi Nakatani, Natsuko Izumi, Yukihide Tomari, Satomi Kuramochi-Miyagawa, Toru Nakano

EMBO reports　Vol. 19　No. 3　2018/03　Research paper (scientific journal)

マウスGTSF1はPIWI-piRNAがターゲットRNAを切断するために重要な因子である

能村 卓慈, 渡部 聡朗, 宮川 さとみ, 竹本 記章, 城本 悠助, 宮東 昭彦, 金井 正美, 田代 文, 宮崎 早月, 刀谷 在美, 中馬 新一郎, 宮崎 純一

生命科学系学会合同年次大会　Vol. 2017年度　p. [1P-0795]　2017/12

Roles of MIWI, MILI and PLD6 in small RNA regulation in mouse growing oocytes.

Yuka Kabayama, Hidehiro Toh, Ami Katanaya, Takayuki Sakurai, Shinichiro Chuma, Satomi Kuramochi-Miyagawa, Yumiko Saga, Toru Nakano, Hiroyuki Sasaki

Nucleic acids research　Vol. 45　No. 9　p. 5387-5398　2017/05/19　Research paper (scientific journal)

MIWI2 as an Effector of DNA Methylation and Gene Silencing in Embryonic Male Germ Cells.

Kanako Kojima-Kita, Satomi Kuramochi-Miyagawa, Ippei Nagamori, Narumi Ogonuki, Atsuo Ogura, Hidetoshi Hasuwa, Takashi Akazawa, Norimitsu Inoue, Toru Nakano

Cell reports　Vol. 16　No. 11　p. 2819-2828　2016/09/13　Research paper (scientific journal)

マウスの始原生殖細胞においてGTSF1は二次piRNAの加工に必須である(Mouse GTSF1 is essential for secondary piRNA processing in goncytes)

能村 卓慈, 竹本 記章, 宮川 さとみ, 渡部 聡朗, 城本 悠助, 宮東 昭彦, 金井 正美, 田代 文, 宮崎 早月, 刀谷 在美, 中馬 新一郎, 仲野 徹, 宮崎 純一

日本生化学会大会・日本分子生物学会年会合同大会講演要旨集　Vol. 88回・38回　p. [2P0796]-[2P0796]　2015/12

piRNAs derived from ancient viral processed pseudogenes as transgenerational sequence-specific immune memory in mammals.

Nicholas F Parrish, Kan Fujino, Yusuke Shiromoto, Yuka W Iwasaki, Hongseok Ha, Jinchuan Xing, Akiko Makino, Satomi Kuramochi-Miyagawa, Toru Nakano, Haruhiko Siomi, Tomoyuki Honda, Keizo Tomonaga

RNA (New York, N.Y.)　Vol. 21　No. 10　p. 1691-703　2015/10　Research paper (scientific journal)

Comprehensive DNA Methylation Analysis of Retrotransposons in Male Germ Cells.

Ippei Nagamori, Hisato Kobayashi, Yusuke Shiromoto, Toru Nishimura, Satomi Kuramochi-Miyagawa, Tomohiro Kono, Toru Nakano

Cell reports　Vol. 12　No. 10　p. 1541-7　2015/09/08　Research paper (scientific journal)

Reply to Shoji and Katsuma.

Satomi Kuramochi-Miyagawa, Toru Nakano

Current biology : CB　Vol. 25　No. 16　2015/08/17

Induction of DNA methylation by artificial piRNA production in male germ cells.

Daisuke Itou, Yusuke Shiromoto, Shin-ya Yukiho, Chika Ishii, Toru Nishimura, Narumi Ogonuki, Atsuo Ogura, Hidetoshi Hasuwa, Yoshitaka Fujihara, Satomi Kuramochi-Miyagawa, Toru Nakano

Current biology : CB　Vol. 25　No. 7　p. 901-6　2015/03/30　Research paper (scientific journal)

HSP90α plays an important role in piRNA biogenesis and retrotransposon repression in mouse.

Tomoko Ichiyanagi, Kenji Ichiyanagi, Ayako Ogawa, Satomi Kuramochi-Miyagawa, Toru Nakano, Shinichiro Chuma, Hiroyuki Sasaki, Heiichiro Udono

Nucleic acids research　Vol. 42　No. 19　p. 11903-11　2014/10/29　Research paper (scientific journal)

DNA methylation in mouse testes.

Satomi Kuramochi-Miyagawa, Kanako Kita-Kojima, Yusuke Shiromoto, Daisuke Ito, Hirotaka Koshima, Toru Nakano

Methods in molecular biology (Clifton, N.J.)　Vol. 1093　p. 97-109　2014　Research paper (scientific journal)

The nuage mediates retrotransposon silencing in mouse primordial ovarian follicles.

Ai Khim Lim, Chanchao Lorthongpanich, Ting Gang Chew, Chin Wee Godwin Tan, Yan Ting Shue, Sathish Balu, Natalia Gounko, Satomi Kuramochi-Miyagawa, Martin M Matzuk, Shinichiro Chuma, Daniel M Messerschmidt, Davor Solter, Barbara B Knowles

Development (Cambridge, England)　Vol. 140　No. 18　p. 3819-25　2013/09　Research paper (scientific journal)

GPAT2, a mitochondrial outer membrane protein, in piRNA biogenesis in germline stem cells.

Yusuke Shiromoto, Satomi Kuramochi-Miyagawa, Akito Daiba, Shinichiro Chuma, Ami Katanaya, Akiko Katsumata, Ken Nishimura, Manami Ohtaka, Mahito Nakanishi, Toshinobu Nakamura, Koichi Yoshinaga, Noriko Asada, Shota Nakamura, Teruo Yasunaga, Kanako Kojima-Kita, Daisuke Itou, Tohru Kimura, Toru Nakano

RNA (New York, N.Y.)　Vol. 19　No. 6　p. 803-10　2013/06　Research paper (scientific journal)

Targeted gene silencing in mouse germ cells by insertion of a homologous DNA into a piRNA generating locus.

Yasuhiro Yamamoto, Toshiaki Watanabe, Yuko Hoki, Kenjiro Shirane, Yufeng Li, Kenji Ichiiyanagi, Satomi Kuramochi-Miyagawa, Atsushi Toyoda, Asao Fujiyama, Masayuki Oginuma, Hitomi Suzuki, Takashi Sado, Toru Nakano, Hiroyuki Sasaki

Genome research　Vol. 23　No. 2　p. 292-9　2013/02　Research paper (scientific journal)

Identification of MIWI-associated Poly(A) RNAs by immunoprecipitation with an anti-MIWI monoclonal antibody.

Takahiro Nishibu, Yukinobu Hayashida, Saori Tani, Sadamu Kurono, Kanako Kojima-Kita, Ryo Ukekawa, Tsutomu Kurokawa, Satomi Kuramochi-Miyagawa, Toru Nakano, Kunio Inoue, Susumu Honda

Bioscience trends　Vol. 6　No. 5　p. 248-61　2012/10

Locus- and domain-dependent control of DNA methylation at mouse B1 retrotransposons during male germ cell development (Genome Research (2011) 21 (2058-2066))

Kenji Ichiyanagi, Yufeng Li, Toshiaki Watanabe, Tomoko Ichiyanagi, Kei Fukuda, Junko Kitayama, Yasuhiro Yamamoto, Satomi Kuramochi-Miyagawa, Toru Nakano, Yukihiro Yabuta, Yoshiyuki Seki, Mitinori Saitou, Hiroyuki Sasaki

Genome Research　Vol. 22　2012/04/01

Locus- and domain-dependent control of DNA methylation at mouse B1 retrotransposons during male germ cell development.

Kenji Ichiyanagi, Yufeng Li, Toshiaki Watanabe, Tomoko Ichiyanagi, Kei Fukuda, Junko Kitayama, Yasuhiro Yamamoto, Satomi Kuramochi-Miyagawa, Toru Nakano, Yukihiro Yabuta, Yoshiyuki Seki, Mitinori Saitou, Hiroyuki Sasaki

Genome research　Vol. 21　No. 12　p. 2058-66　2011/12　Research paper (scientific journal)

Tudor domain containing 7 (Tdrd7) is essential for dynamic ribonucleoprotein (RNP) remodeling of chromatoid bodies during spermatogenesis.

Takashi Tanaka, Mihoko Hosokawa, Vasily V Vagin, Michael Reuter, Eri Hayashi, Ayako L Mochizuki, Kouichi Kitamura, Hidenori Yamanaka, Gen Kondoh, Katsuya Okawa, Satomi Kuramochi-Miyagawa, Toru Nakano, Ravi Sachidanandam, Gregory J Hannon, Ramesh S Pillai, Norio Nakatsuji, Shinichiro Chuma

Proceedings of the National Academy of Sciences of the United States of America　Vol. 108　No. 26　p. 10579-84　2011/06/28　Research paper (scientific journal)

Role for piRNAs and noncoding RNA in de novo DNA methylation of the imprinted mouse Rasgrf1 locus.

Toshiaki Watanabe, Shin-ichi Tomizawa, Kohzoh Mitsuya, Yasushi Totoki, Yasuhiro Yamamoto, Satomi Kuramochi-Miyagawa, Naoko Iida, Yuko Hoki, Patrick J Murphy, Atsushi Toyoda, Kengo Gotoh, Hitoshi Hiura, Takahiro Arima, Asao Fujiyama, Takashi Sado, Tatsuhiro Shibata, Toru Nakano, Haifan Lin, Kenji Ichiyanagi, Paul D Soloway, Hiroyuki Sasaki

Science (New York, N.Y.)　Vol. 332　No. 6031　p. 848-52　2011/05/13

MITOPLD is a mitochondrial protein essential for nuage formation and piRNA biogenesis in the mouse germline.

Toshiaki Watanabe, Shinichiro Chuma, Yasuhiro Yamamoto, Satomi Kuramochi-Miyagawa, Yasushi Totoki, Atsushi Toyoda, Yuko Hoki, Asao Fujiyama, Tatsuhiro Shibata, Takashi Sado, Toshiaki Noce, Toru Nakano, Norio Nakatsuji, Haifan Lin, Hiroyuki Sasaki

Developmental cell　Vol. 20　No. 3　p. 364-75　2011/03/15　Research paper (scientific journal)

MVH in piRNA processing and gene silencing of retrotransposons.

Satomi Kuramochi-Miyagawa, Toshiaki Watanabe, Kengo Gotoh, Kana Takamatsu, Shinichiro Chuma, Kanako Kojima-Kita, Yusuke Shiromoto, Noriko Asada, Atsushi Toyoda, Asao Fujiyama, Yasushi Totoki, Tatsuhiro Shibata, Tohru Kimura, Norio Nakatsuji, Toshiaki Noce, Hiroyuki Sasaki, Toru Nakano

Genes & development　Vol. 24　No. 9　p. 887-92　2010/05　Research paper (scientific journal)

A broadly conserved pathway generates 3'UTR-directed primary piRNAs.

Nicolas Robine, Nelson C Lau, Sudha Balla, Zhigang Jin, Katsutomo Okamura, Satomi Kuramochi-Miyagawa, Michael D Blower, Eric C Lai

Current biology : CB　Vol. 19　No. 24　p. 2066-76　2009/12/29

The TDRD9-MIWI2 complex is essential for piRNA-mediated retrotransposon silencing in the mouse male germline.

Masanobu Shoji, Takashi Tanaka, Mihoko Hosokawa, Michael Reuter, Alexander Stark, Yuzuru Kato, Gen Kondoh, Katsuya Okawa, Takeshi Chujo, Tsutomu Suzuki, Kenichiro Hata, Sandra L Martin, Toshiaki Noce, Satomi Kuramochi-Miyagawa, Toru Nakano, Hiroyuki Sasaki, Ramesh S Pillai, Norio Nakatsuji, Shinichiro Chuma

Developmental cell　Vol. 17　No. 6　p. 775-87　2009/12　Research paper (scientific journal)

Gtsf1/Cue110, a gene encoding a protein with two copies of a CHHC Zn-finger motif, is involved in spermatogenesis and retrotransposon suppression in murine testes.

Takuji Yoshimura, Shuichi Toyoda, Satomi Kuramochi-Miyagawa, Tatsushi Miyazaki, Satsuki Miyazaki, Fumi Tashiro, Eiji Yamato, Toru Nakano, Jun-Ichi Miyazaki

Developmental biology　Vol. 335　No. 1　p. 216-27　2009/11/01

Associations between PIWI proteins and TDRD1/MTR-1 are critical for integrated subcellular localization in murine male germ cells.

Kanako Kojima, Satomi Kuramochi-Miyagawa, Shinichiro Chuma, Takashi Tanaka, Norio Nakatsuji, Tohru Kimura, Toru Nakano

Genes to cells : devoted to molecular & cellular mechanisms　Vol. 14　No. 10　p. 1155-65　2009/10　Research paper (scientific journal)

RNA silencing in germlines--exquisite collaboration of Argonaute proteins with small RNAs for germline survival.

Mikiko C Siomi, Satomi Kuramochi-Miyagawa

Current opinion in cell biology　Vol. 21　No. 3　p. 426-34　2009/06

MILI, a PIWI-interacting RNA-binding protein, is required for germ line stem cell self-renewal and appears to positively regulate translation.

Yingdee Unhavaithaya, Yi Hao, Ergin Beyret, Hang Yin, Satomi Kuramochi-Miyagawa, Toru Nakano, Haifan Lin

The Journal of biological chemistry　Vol. 284　No. 10　p. 6507-19　2009/03/06

Endogenous siRNAs from naturally formed dsRNAs regulate transcripts in mouse oocytes.

Toshiaki Watanabe, Yasushi Totoki, Atsushi Toyoda, Masahiro Kaneda, Satomi Kuramochi-Miyagawa, Yayoi Obata, Hatsune Chiba, Yuji Kohara, Tomohiro Kono, Toru Nakano, M Azim Surani, Yoshiyuki Sakaki, Hiroyuki Sasaki

Nature　Vol. 453　No. 7194　p. 539-43　2008/05/22

DNA methylation of retrotransposon genes is regulated by Piwi family members MILI and MIWI2 in murine fetal testes.

Satomi Kuramochi-Miyagawa, Toshiaki Watanabe, Kengo Gotoh, Yasushi Totoki, Atsushi Toyoda, Masahito Ikawa, Noriko Asada, Kanako Kojima, Yuka Yamaguchi, Takashi W Ijiri, Kenichiro Hata, En Li, Yoichi Matsuda, Tohru Kimura, Masaru Okabe, Yoshiyuki Sakaki, Hiroyuki Sasaki, Toru Nakano

Genes & development　Vol. 22　No. 7　p. 908-17　2008/04/01

[PiRNA in spermatogenesis].

Satomi Kuramochi-Miyagawa, Kengo Gotoh, Toru Kimura, Toru Nakano

Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme　Vol. 52　No. 16 Suppl　p. 2097-101　2007/12

Precancerous stem cells have the potential for both benign and malignant differentiation.

Li Chen, Rulong Shen, Yin Ye, Xin-An Pu, Xingluo Liu, Wenrui Duan, Jing Wen, Jason Zimmerer, Ying Wang, Yan Liu, Larry C Lasky, Nyla A Heerema, Danilo Perrotti, Keiko Ozato, Satomi Kuramochi-Miyagawa, Toru Nakano, Allen J Yates, William E Carson 3rd, Haifan Lin, Sanford H Barsky, Jian-Xin Gao

PloS one　Vol. 2　No. 3　2007/03/14

Characterization of the piRNA complex from rat testes.

Nelson C Lau, Anita G Seto, Jinkuk Kim, Satomi Kuramochi-Miyagawa, Toru Nakano, David P Bartel, Robert E Kingston

Science (New York, N.Y.)　Vol. 313　No. 5785　p. 363-7　2006/07/21

A novel class of small RNAs bind to MILI protein in mouse testes.

Alexei Aravin, Dimos Gaidatzis, Sébastien Pfeffer, Mariana Lagos-Quintana, Pablo Landgraf, Nicola Iovino, Patricia Morris, Michael J Brownstein, Satomi Kuramochi-Miyagawa, Toru Nakano, Minchen Chien, James J Russo, Jingyue Ju, Robert Sheridan, Chris Sander, Mihaela Zavolan, Thomas Tuschl

Nature　Vol. 442　No. 7099　p. 203-7　2006/07/13

Mili, a mammalian member of piwi family gene, is essential for spermatogenesis.

Satomi Kuramochi-Miyagawa, Tohru Kimura, Takashi W Ijiri, Taku Isobe, Noriko Asada, Yukiko Fujita, Masahito Ikawa, Naomi Iwai, Masaru Okabe, Wei Deng, Haifan Lin, Yoichi Matsuda, Toru Nakano

Development (Cambridge, England)　Vol. 131　No. 4　p. 839-49　2004/02

Two mouse piwi-related genes: Miwi and mili

Satomi Kuramochi-Miyagawa, Tohru Kimura, Kentaro Yomogida, Asato Kuroiwa, Yuko Tadokoro, Yukiko Fujita, Masatake Sato, Yoichi Matsuda, Toru Nakano

Mechanisms of Development　Vol. 108　No. 1-2　p. 121-133　2001　Research paper (scientific journal)

Deficiency of a STE20/PAK family kinase LOK leads to the acceleration of LFA-1 clustering and cell adhesion of activated lymphocytes

Junji Endo, Noriko Toyama-Sorimachi, Choji Taya, Satomi Kuramochi-Miyagawa, Kisaburo Nagata, Keisuke Kuida, Tohru Takashi, Hiromichi Yonekawa, Yasuyuki Yoshizawa, Nobuyuki Miyasaka, Hajime Karasuyama

FEBS Letters　Vol. 468　No. 2-3　p. 234-238　2000/02/25　Research paper (scientific journal)

The regulation of membrane cofactor protein (CD46) expression by the 3' untranslated region in transgenic mice.

Miyagawa S, Mikata S, Tanaka H, Ikawa M, Kominami K, Seya T, Nishimune Y, Shirakura R, Okabe M

Biochem Biophys Res Commun.　Vol. 28;233(3):829-33　1997/04　Research paper (scientific journal)

The regulation of membrane cofactor protein (CD46) expression in transgenic mice: the importance of the first 125 BP of the 3' untranslated region.

Miyagawa S, Ikawa M, Kominami K, Tanaka H, Mikata S, Matsuda H, Seya T, Shirakura R, Okabe M

Transplant Proc.　Vol. 29(1-2):941-2　1997/02　Research paper (scientific journal)