keyword Optical microscopy
Osaka University Graduate School of Engineering
2016/04 - 2021/03
Osaka University School of Engineering
2012/04 - 2016/03
日本光学会 光科学および光技術調査委員会(関西)
2024/04 - Present
Life sciences / Biomedical engineering /
Nanotechnology/Materials / Optical engineering and photonics /
若手講演賞
久保俊貴 日本分光学会 2024/06
ベストイメージング賞エビデント（オリンパス）賞
久保俊貴 日本バイオイメージング学会 2022/09
研究科長表彰
久保俊貴 2017/03
Raman flow cytometry using time-delay integration
Matthew Lindley, Toshiki Kubo, Stéphanie Devineau, Menglu Li, Jing Qiao, Takuya Yashiro, Shiroh Iwanaga, Kazuyo Moro, Katsumasa Fujita
Optica 2025/04/02 Research paper (scientific journal)
Alkyne-tag Raman imaging and sensing of bioactive compounds
Kosuke Dodo, William J. Tipping, Hiroyuki Yamakoshi, Syusuke Egoshi, Toshiki Kubo, Yasuaki Kumamoto, Karen Faulds, Duncan Graham, Katsumasa Fujita, Mikiko Sodeoka
Nature Reviews Methods Primers 2025/03/27 Research paper (scientific journal)
Selective-plane-activation structured illumination microscopy
Kenta Temma, Ryosuke Oketani, Toshiki Kubo, Kazuki Bando, Shunsuke Maeda, Kazunori Sugiura, Tomoki Matsuda, Rainer Heintzmann, Tatsuya Kaminishi, Koki Fukuda, Maho Hamasaki, Takeharu Nagai, Katsumasa Fujita
Nature Methods 2024/05 Research paper (scientific journal)
Publisher: Cold Spring Harbor Laboratory
On-the-fly Raman microscopy guaranteeing the accuracy of discrimination
Koji Tabata, Hiroyuki Kawagoe, J. Nicholas Taylor, Kentaro Mochizuki, Toshiki Kubo, Jean-Emmanuel Clement, Yasuaki Kumamoto, Yoshinori Harada, Atsuyoshi Nakamura, Katsumasa Fujita, Tamiki Komatsuzaki
Proceedings of the National Academy of Sciences Vol. 121 No. 12 2024/03/14 Research paper (scientific journal)
Publisher: Proceedings of the National Academy of Sciences
Time-deterministic cryo-optical microscopy
M. Yamanaka, K. Tsuji, Y. Kumamoto, S. Tamura, W. Miyamura, T. Kubo, K. Mizushima, K. Kono, H. Hirano, K. Sugiura, S. Fukushima, T. Kunimoto, K. Nishida, K. Mochizuki, Y. Harada, N. I. Smith, R. Heintzmann, T. Nagai, H. Tanaka, Katsumasa Fujita
bioRχiv 2023/08/03 Research paper (scientific journal)
Publisher: Cold Spring Harbor Laboratory
Bessel-beam illumination Raman microscopy
KAZUKI BANDO, SHUMPEI YABUUCHI, MENGLU LI, TOSHIKI KUBO, RYOSUKE OKETANI, NICHOLAS I. SMITH, AND KATSUMASA FUJITA
Biomedical Optics Express Vol. 13 No. 6 p. 3161-3161 2022/06/01 Research paper (scientific journal)
Publisher: Optica Publishing Group
Saturated-excitation image scanning microscopy
Kenta Temma, Ryosuke Oketani, René Lachmann, Toshiki Kubo, Nicholas I. Smith, Rainer Heintzmann, Katsumasa Fujita
Optics Express Vol. 30 No. 8 p. 13825-13825 2022/04/11 Research paper (scientific journal)
Publisher: Optica Publishing Group
Visible-Wavelength Multiphoton Activation Confocal Microscopy
Toshiki Kubo, Kenta Temma, Kazunori Sugiura, Hajime Shinoda, Kai Lu, Nicholas Isaac Smith, Tomoki Matsuda, Takeharu Nagai, Katsumasa Fujita
ACS Photonics Vol. 8 No. 9 p. 2666-2673 2021/09/15 Research paper (scientific journal)
Publisher: American Chemical Society ({ACS})
Hyperspectral two-photon excitation microscopy using visible wavelength
Toshiki Kubo, Kenta Temma, Nicholas I. Smith, Kai Lu, Tomoki Matsuda, Takeharu Nagai, Katsumasa Fujita
Optics Letters Vol. 46 No. 1 p. 37-37 2021/01/01 Research paper (scientific journal)
Publisher: Optica Publishing Group
Wavefront‐sensorless adaptive optics with a laser‐free spinning disk confocal microscope
Syed Asad Hussain, Toshiki Kubo, Nicholas Hall, Dalia Gala, Karen Hampson, Richard Parton, Mick A. Phillips, Matthew Wincott, Katsumasa Fujita, Ilan Davis, Ian Dobbie, Martin J. Booth
Journal of Microscopy Vol. 288 p. 106-116 2020/11/20 Research paper (scientific journal)
Publisher: Wiley
Visible-wavelength two-photon excitation microscopy with multifocus scanning for volumetric live-cell imaging
Ryosuke Oketani, Haruka Suda, Kumiko Uegaki, Toshiki Kubo, Tomoki Matsuda, Masahito Yamanaka, Yoshiyuki Arai, Nicholas I. Smith, Takeharu Nagai, Katsumasa Fujita
Journal of Biomedical Optics Vol. 25 No. 01 p. 1-1 2019/11/05 Research paper (scientific journal)
Publisher: {SPIE}-Intl Soc Optical Eng
Saturated-excitation image scanning microscopy for super-resolution fluorescence imaging
Kenta Temma, Ryosuke Oketani, René Lachmann, Toshiki Kubo, Nicholas I. Smith, Rainer Heintzmann, Katsumasa Fujita
Biomedical Imaging and Sensing Conference 2023/09/20
Publisher: SPIE
光学顕微観察下における細胞内ダイナミクスの急速凍結固定技術の開発
山中真仁, 辻康介, 辻康介, 熊本康昭, 熊本康昭, 田村昌子, 宮村和奏, 久保俊貴, 久保俊貴, 水島健太, 水島健太, 河野駆, 平野花咲, 杉浦一徳, 福島俊一, 國本拓実, 西田健太郎, 望月健太郎, 原田義規, SMITH Nicholas I., 永井健治, 永井健治, 田中秀央, 藤田克昌, 藤田克昌, 藤田克昌
バイオイメージング Vol. 32 No. 2 2023
Nonlinear confocal microscopy using visible-wavelength two-photon activation of fluorescent proteins
Toshiki Kubo, Kenta Temma, Kazunori Sugiura, Hajime Shinoda, Kai Lu, Nicholas I. Smith, Tomoki Matsuda, Takeharu Nagai, Katsumasa Fujita
Multiphoton Microscopy in the Biomedical Sciences XXII 2022/03/07
Publisher: SPIE
Nonlinear image scanning microscopy using saturated excitation
Kenta Temma, Ryosuke Oketani, René Lachmann, Toshiki Kubo, Nicholas I. Smith, Rainer Heintzmann, Katsumasa Fujita
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIX 2022/03/02
Publisher: SPIE
Hyperspectral fluorescence imaging by using visible-wavelength two-photon excitation
Toshiki Kubo, Kenta Temma, Nicholas I. Smith, Lu Kai, Tomoki Matsuda, Takeharu Nagai, Katsumasa Fujita
Proc. SPIE 11521, Biomedical Imaging and Sensing Conference 2020 2020/06/15
Publisher: SPIE
Sensorless adaptive optics with a laser free spinning disk confocal microscope
Syed Asad Hussain, Toshiki Kubo, Nicholas Hall, Karen Hampson, Mick Phillips, Dalia Gala, Ian M. Dobbie, Jacopo Antonello, Matthew Wincott, Ilan Davis, Martin J. Booth
Proc. SPIE 11248, Adaptive Optics and Wavefront Control for Biological Systems VI 2020/02/17
Publisher: SPIE
2光子励起とその飽和を用いた超解像蛍光イメージング—Saturated two-photon excitation microscopy for super-resolution fluorescence imaging—光・量子デバイス研究会・バイオメディカルフォトニクス応用
桶谷 亮介, 土井 厚志, ミヤサキ ケートリン, 久保 俊貴, 名和 靖矩, 河田 聡, 藤田 克昌
電気学会研究会資料. OQD = The papers of technical meeting on optical and quantum devices, IEE Japan / 光・量子デバイス研究会 [編] Vol. 2018 No. 1-9 p. 33-36 2018/03/05
Publisher: 東京 : 電気学会
Saturated two-photon excitation microscopy for super-resolution fluorescence imaging
桶谷亮介, 土井厚志, MIYASAKI Katelyn, 久保俊貴, 名和靖矩, 河田聡, 藤田克昌
電気学会研究会資料 No. OQD-18-001-009 2018
可視波長域2光子励起を用いた蛍光分光顕微鏡の開発
久保俊貴, 望月健太郎, 渡辺梢, 藤井俊輔, 新井由之, 畔堂一樹, SMITH Nicholas I., 河田聡, 永井健治, 藤田克昌
応用物理学会春季学術講演会講演予稿集(CD-ROM) Vol. 64th 2017
Saturated two-photon excitation microscopy for super-resolution imaging
桶谷亮介, 桶谷亮介, 土井厚志, MIYASAKI Katelyn, 久保俊貴, 名和靖矩, 名和靖矩, 河田聡, 藤田克昌, 藤田克昌
Optics & Photonics Japan講演予稿集(CD-ROM) Vol. 2017 2017
Super-resolution microscopy by inducing nonlinearity in fluorescence emission
Toshiki Kubo
The 13 Asia-Pacific Laser Symposium 2025/05/16
On-the-fly slit-scanning confocal Raman microscope guaranteeing the accuracy of discrimination
Toshiki Kubo, Koji Tabata, Hiroyuki Kawagoe, James Nicholas Taylor, Jean-Emmanuel Clemen, Ryoya Kondo, Yoshinori Harada, Atsuyoshi Nakamura, Katsumasa Fujita, Tamiki Komatsuzaki
Focus on Microscopy 2025 2025/04/14
非線形超解像光学顕微鏡
久保俊貴
一般社団法人レーザー学会学術講演会第45回年次大会 2025/01/22
共焦点On-the-flyラマン顕微鏡による迅速診断
久保俊貴, 田畑公次, 川越寛之, James Nicholas Talyer, Jean-Emmanuel Clemen, 原田義規, 中村篤祥, 藤田克昌, 小松崎民樹
レーザ顕微鏡研究会第49回講演会・シンポジウム 2024/12/09
Raman flow cytometry using time delay integration and light-sheet illumination
T. Kubo, M. Lindley, S. Devineau, M. Li, K. Fujita
The 22nd Annual Meeting of The Japan Association of Medical Spectroscopy & Biomedical Raman Imaging Workshop 2024 2024/11/24
Spontaneous Raman flow cytometry using time delay integration
T. Kubo, M. Lindley, S. Devineau, M. Li, K. Fujita
ICORS 2024 2024/08/01
Time delay integrationを用いたラマンフローサイトメーターの開発
久保俊貴, Matthew Lindley, Stephanie Devineau, Menglu Li, 藤田克昌
2024年度日本分光学会年次講演会 2024/06/19
On-the-fly slit-scanning confocal Raman microscope guaranteeing the accuracy of discrimination
T. Kubo, K. Tabata, H. Kawagoe, J. N. Taylor, K. Mochizuki, B. Yan, J. E. Clemen, Y. Kumamoto, Y. Harada, A. Nakamura, K. Fujita, T. Komatsuzaki
SPIE Photonics West 2024 2024/01/27
TDIを用いたラマンフローサイトメトリー
久保俊貴, Matthew Lindley, Stephanie Devineau, Menglu Li, 藤田克昌
第84回応用物理学会秋季学術講演会 2023/09/20
High-speed Raman spectroscopic diagnosis guaranteeing accuracy by reinforcement learning
T. Kubo, K. Tabata, H. Kawagoe, J. N. Taylor, K. Mochizuki, J. E. Clemen, Y. Kumamoto, Y. Harada, A. Nakamura, K. Fujita, T. Komatsuzaki
Optics & Photonics International Congress (OPIC) 2023 2023/04/19
On-the-flyラマン顕微鏡による迅速診断
田畑公次, 川越寛之, James Nicholas Talyer, 望月健太郎, 久保俊貴, Jean-Emmanuel Clemen, 熊本康昭, 原田義規, 中村篤祥, 藤田克昌, 小松崎民樹
レーザ顕微鏡研究会第47回講演会・シンポジウム (SLM-47) 2022/11/25
バンディットラマン顕微鏡：強化学習を用いた迅速診断
田畑公次, 川越寛之, James Nicholas Talyer, 望月健太郎, 久保俊貴, Jean-Emmanuel Clement, 熊本康昭, 原田義規, 中村篤祥, 藤田克昌, 小松崎民樹
2022年度公益社団法人日本分光学会年次講演会 2022/10/13
可視光２光子励起を用いた共焦点顕微鏡の高速化・高空間分解能化
久保 俊貴, 天満 健太, 桶谷 亮介, 杉浦 一徳, 魯 慨, Nicholas I.Smith, 松田 知己, 永井 健治, 藤田 克昌
第31回バイオイメージング学会 2022/09
Multi-photon activation of fluorescent proteins using visible wavelength for high-resolution imaging
T. Kubo, K. Temma, K. Sugiura, H. Shinoda, K. Lu, N. I. Smith, T. Matsuda, T. Nagai, K. Fujita
Conference on Lasers and Electro-Optics Pacific Rim (CLEO-PR) 2022 2022/08
Super-resolution confocal microscopy using nonlinear photo-switching of fluorescent proteins
Toshiki Kubo
Global Nanophotonics 2022 2022/03
Nonlinear confocal microscopy using visible-wavelength two-photon activation of fluorescent proteins
T. Kubo, K. Temma, T. Matsuda, K. Sugiura, K. Lu, N. I. Smith, T. Nagai, K. Fujita
SPIE Photonics West 2022 2022/01
On-the-fly Raman image microscopy by reinforcement machine learning: Acceleration of medical diagnosis
K. Tabata, H. Kawagoe, J. N. Taylor, K. Mochizuki, T. Kubo, B. Yan, J. E. Clement, Y. Kumamoto, Y. Harada, A. Nakamura, K. Fujita, T. Komatsuzaki
SPIE Photonics West 2022 2022/01
可視光による２光子励起を用いた高次非線形蛍光顕微鏡
久保俊貴, 天満健太, 杉浦一徳, 魯慨, 松田知己, スミスニコラス, 永井健治, 藤田克昌
レーザー顕微鏡研究会 2021 2021/07
Sensor-less adaptive optics in aperture correlation confocal microscopy
T. Kubo, S. A. Hussain, N. Hall, K. Hampson, M. A. Phillips, D. Gala, I. Dobbie, J. Antonello, M. Wincott, K. Fujita, I. Davis, M. J. Booth
2020/09
可視光２光子吸収を用いた高次非線形蛍光顕微鏡
久保俊貴, 天満健太, 松田知己, 杉浦一徳, 魯慨, スミスニコラス, 永井健治, 藤田克昌
第81回応用物理学会秋季学術講演会 2020/09
Hyperspectral fluorescence imaging by using visible-wavelength two-photon excitation
T. Kubo, K. Temma, N. I. Smith, K. Lu, T. Matsuda, T. Nagai, K. Fujita
Optics & Photonics International Congress (OPIC) 2020 2020/04
可視光２光子励起を用いたハイパースペクトラルイメージング
久保俊貴, 天満健太, スミスニコラス, 魯 慨, 松田知己, 永井健治, 藤田克昌
日本光学会年次学術講演会 Optics & Photonics Japan 2019 2019/12
Visible-Wavelength Two-Photon Excitation for Multicolor Fluorescent Protein Imaging with Improved Image Contrast
T. Kubo, R. Oketani, N. I. Smith, Y. Arai, T.Matsuda, T. Nagai, K. Fujita
Focus on Microscopy 2019 2019/04
Spectral imaging with visible-wavelength two-photon excitation
T. Kubo, K. Mochizuki, N. I. Smith, K. Fujita
The International Symposium for Materials Scientists “Inspiration for Innovation by Interaction” (ISMS III) 2018/12
Visible-wavelength two-photon excitation microscopy with spectral detection
T. Kubo, K. Mochizuki, R. Oketani, K. Watanabe, S. Fujii, Y. Arai, K. Bando, N. I. Smith, S. Kawata, T. Nagai, K. Fujita
The 24th Congress of the International Commission for Optics (ICO24) 2017/08
可視波長域2光子励起を用いた蛍光分光顕微鏡の開発
久保俊貴, 望月健太郎, 渡辺梢, 藤井俊輔, 新井由之, 畔堂一樹, スミスニコラス, 河田聡, 永井健治, 藤田克昌
第64回応用物理学会春季学術講演会 2017/03/17
