Characterization of Bombyx mori N-acetylglucosaminyltransferase II splicing variants

Hiroyuki Kajiura, Yugo Nakamura, Mari Nishimura, Takao Ohashi, Ryo Misaki, Kazuhito Fujiyama

Biochemical and Biophysical Research Communications　Vol. 529　No. 2　p. 404-410　2020/08　Research paper (scientific journal)

Elucidation of rubber biosynthesis and accumulation in the rubber producing shrub, guayule (Parthenium argentatum Gray).

Kajiura H, Suzuki N, Mouri H, Watanabe N, Nakazawa Y

Planta　Vol. 247　No. 2　p. 513-526-526　2018/04　Research paper (scientific journal)

Two Eucommia farnesyl diphosphate synthases exhibit distinct enzymatic properties leading to end product preferences.

Kajiura H, Suzuki N, Tokumoto Y, Yoshizawa T, Takeno S, Fujiyama K, Kaneko Y, Matsumura H, Nakazawa Y

Biochimie　Vol. 139　p. 95-106-106　2017/04　Research paper (scientific journal)

Im"plant"ing of mammalian glycosyltransferase gene into plant suspension-cultured cells using Agrobacterium-mediated transformation.

Kajiura H, Fujiyama K

Methods Mol Biol.　Vol. 1321　p. 225-232-232　2015/04　Research paper (scientific journal)

Sialylation potentials of the silkworm, Bombyx mori; B.mori possesses an active α2,6-sialyltransferase.

Kajiura H, Hamaguchi Y, Mizushima H, Misaki R, Fujiyama K

Glycobiology　Vol. 25　No. 12　p. 1441-1453-1453　2015/04　Research paper (scientific journal)

N-Glycosylation and N-Glycan moieties of CTB expressed in rice seeds.

Kajiura H, Wasai M, Kasahara S, Takaiwa F, Fujiyama K

Mol Biotechnol.　Vol. 54　No. 3　p. 784-794-794　2013/04　Research paper (scientific journal)

Arabidopsis beta 1,2-xylosyltransferase: Substrate specificity and participation in the plant-specific N-glycosylation pathway

Hiroyuki Kajiura, Toru Okamoto, Ryo Misaki, Yoshiharu Matsuura, Kazuhito Fujiyama

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　Vol. 113　No. 1　p. 48-54　2012/01　Research paper (scientific journal)

Stable coexpression of two human sialylation enzymes in plant suspension-cultured tobacco cells.

Kajiura H, Misaki R, Fujiyama K, Seki T

J Biosci Bioeng.　Vol. 111　No. 4　p. 471-477-477　2011/04　Research paper (scientific journal)

Arabidopsis thaliana ALG3 mutant synthesizes immature oligosaccharides in the ER and accumulates unique N-glycans.

Kajiura H, Seki T, Fujiyama K

Glycobiology　Vol. 20　No. 6　p. 736-751-751　2010/04　Research paper (scientific journal)

Two Arabidopsis thaliana Golgi alpha-mannosidase I enzymes are responsible for plant N-glycan maturation

Hiroyuki Kajiura, Hisashi Koiwa, Yoshihisa Nakazawa, Atsushi Okazawa, Akio Kobayashi, Tatsuji Seki, Kazuhito Fujiyama

GLYCOBIOLOGY　Vol. 20　No. 2　p. 235-247　2010/02　Research paper (scientific journal)

Expression of an endo-rhamnogalacturonase from Aspergillus aculeatus enhances release of Arabidopsis transparent mucilage.

Takao Ohashi, Yurika Mabira, Yutaro Mitsuyoshi, Hiroyuki Kajiura, Ryo Misaki, Takeshi Ishimizu, Kazuhito Fujiyama

Journal of bioscience and bioengineering　Vol. 138　No. 1　p. 73-82　2024/04/19　Research paper (scientific journal)

Reconstitution of (1→3)-β-D-glucans measurement system using recombinant Limulus polyphemus Factor G

Yotaro Yamamoto, Hiroyuki Kajiura, Hiroki Fukuchi, Takahiro Nishibu, Kazuhito Fujiyama, Takeshi Kitagawa

Applied Microbiology and Biotechnology　Vol. 107　No. 24　p. 7463-7473　2023/10/26　Research paper (scientific journal)

The apiosyltransferase celery UGT94AX1 catalyzes the biosynthesis of the flavone glycoside apiin

Maho Yamashita, Tae Fujimori, Song An, Sho Iguchi, Yuto Takenaka, Hiroyuki Kajiura, Takuya Yoshizawa, Hiroyoshi Matsumura, Masaru Kobayashi, Eiichiro Ono, Takeshi Ishimizu

Plant Physiology　Vol. 193　No. 3　p. 1758-1771　2023/07/11　Research paper (scientific journal)

Production and N-glycan engineering of Varlilumab in Nicotiana benthamiana.

Kim Dua Nguyen, Hiroyuki Kajiura, Ryo Kamiya, Takahiro Yoshida, Ryo Misaki, Kazuhito Fujiyama

Frontiers in plant science　Vol. 14　p. 1215580-1215580　2023　Research paper (scientific journal)

Effects of various disaccharide adaptations on recombinant IgA1 production in CHO-K1 suspension cells

John Benson D. Choa, Tadahiro Sasaki, Hiroyuki Kajiura, Kazuyoshi Ikuta, Kazuhito Fujiyama, Ryo Misaki

Cytotechnology　Vol. 75　No. 3　p. 219-229　2023　Research paper (scientific journal)

Squaryl group-modified UDP analogs as inhibitors of the endoplasmic reticulum-resident folding sensor enzyme UGGT

Junpei Abe, Yoichi Takeda, Takashi Kikuma, Yasuhiko Kizuka, Hiroyuki Kajiura, Yasuhiro Kajihara, Yukishige Ito

Chemical Communications　Vol. 59　No. 19　p. 2803-2806　2023　Research paper (scientific journal)

Impact of glycoengineering and antidrug antibodies on the anticancer activity of a plant-made lectin-Fc fusion protein.

Matthew Dent, Katarina L Mayer, Noel Verjan Garcia, Haixun Guo, Hiroyuki Kajiura, Kazuhito Fujiyama, Nobuyuki Matoba

Plant biotechnology journal　Vol. 20　No. 11　p. 2217-2230　2022/11　Research paper (scientific journal)

Mamestra brassicae NIAS-Mb-32 cell strain 2g2 enables high-yield recombinant protein production in baculovirus-free and baculovirus-based insect cell expression.

Yotaro Yamamoto, Hiroyuki Kajiura, Takahiro Nishibu, Kazuhito Fujiyama

Journal of bioscience and bioengineering　Vol. 134　No. 5　p. 432-440　2022/11　Research paper (scientific journal)

High-level transient production of a protease-resistant mutant form of human basic fibroblast growth factor in Nicotiana benthamiana leaves.

Edjohn Aaron Macauyag, Hiroyuki Kajiura, Takao Ohashi, Ryo Misaki, Kazuhito Fujiyama

Plant biotechnology (Tokyo, Japan)　Vol. 39　No. 3　p. 291-301　2022/09/25　Research paper (scientific journal)

Production of recombinant β-glucocerebrosidase in wild-type and glycoengineered transgenic Nicotiana benthamiana root cultures with different N-glycan profiles

Naphatsamon Uthailak, Hiroyuki Kajiura, Ryo Misaki, Kazuhito Fujiyama

Journal of Bioscience and Bioengineering　Vol. 133　No. 5　p. 481-488　2022/05　Research paper (scientific journal)

Establishment of serum-free adapted Chinese hamster ovary cells with double knockout of GDP-mannose-4,6-dehydratase and GDP-fucose transporter.

Ryo Misaki, Masashi Iwasaki, Hiroki Takechi, Noriko Yamano-Adachi, Takao Ohashi, Hiroyuki Kajiura, Kazuhito Fujiyama

Cytotechnology　Vol. 74　No. 1　p. 163-179　2022/02　Research paper (scientific journal)

Analysis of N-glycan profile of Arabidopsis alg3 cell culture.

Ratna Sariyatun, Hiroyuki Kajiura, Juthamard Limkul, Ryo Misaki, Kazuhito Fujiyama

Plant biotechnology (Tokyo, Japan)　Vol. 38　No. 4　p. 463-467　2021/12/25　Research paper (scientific journal)

Improved assay system for acidic peptide: N-glycanase (aPNGase) activity in plant extracts

Chiharu Yamamoto, Mikako Ogura, Ryota Uemura, Maeda Megumi, Hiroyuki Kajiura, Ryo Misaki, Kazuhito Fujiyama, Yoshinobu Kimura

Analytical Biochemistry　Vol. 634　p. 114367-114367　2021/09　Research paper (scientific journal)

Production of Human Acid-Alpha Glucosidase With a Paucimannose Structure by Glycoengineered Arabidopsis Cell Culture

Ratna Sariyatun, Florence, Hiroyuki Kajiura, Takao Ohashi, Ryo Misaki, Kazuhito Fujiyama

Frontiers in Plant Science　Vol. 12　p. 703020-703020　2021/07/14　Research paper (scientific journal)

Transient Production of Human β-Glucocerebrosidase With Mannosidic-Type N-Glycan Structure in Glycoengineered Nicotiana benthamiana Plants

Naphatsamon Uthailak, Hiroyuki Kajiura, Ryo Misaki, Kazuhito Fujiyama

Frontiers in Plant Science　Vol. 12　p. 683762-683762　2021/06/07　Research paper (scientific journal)

Functional characterization and overexpression of Δ12-desaturase in the oleaginous yeast Rhodotorula toruloides for production of linoleic acid-rich lipids

Chih-Chan Wu, Takao Ohashi, Hiroyuki Kajiura, Yu Sato, Ryo Misaki, Kohsuke Honda, Savitree Limtong, Kazuhito Fujiyama

Journal of Bioscience and Bioengineering　Vol. 131　No. 6　p. 631-639　2021/06　Research paper (scientific journal)

Direct evidence of cytosolic PNGase activity in Arabidopsis thaliana: in vitro assay system for plant cPNGase activity

Sahoko Shirai, Ryota Uemura, Megumi Maeda, Hiroyuki Kajiura, Ryo Misaki, Kazuhito Fujiyama, Yoshinobu Kimura

Bioscience, Biotechnology, and Biochemistry　Vol. 85　No. 6　p. 1460-1463　2021/03/16　Research paper (scientific journal)

Structure and Biological Functions of Plant Glycans and Polysaccharides

Ryo Misaki, Kazuhito Fujiyama, Hiroyuki Kajiura

Comprehensive Glycoscience　p. 93-109　2021　Part of collection (book)

Alkaline hydrolysis of coconut pith: process optimization, enzymatic saccharification, and nitrobenzene oxidation of Kraft lignin

Gundupalli, M.P., Kajiura, H., Ishimizu, T., Bhattacharyya, D.

Biomass Conversion and Biorefinery　2020/07/24　Research paper (scientific journal)

Diversity of Pectin Rhamnogalacturonan I Rhamnosyltransferases in Glycosyltransferase Family 106

Bussarin Wachananawat, Takeshi Kuroha, Yuto Takenaka, Hiroyuki Kajiura, Satoshi Naramoto, Ryusuke Yokoyama, Kimitsune Ishizaki, Kazuhiko Nishitani, Takeshi Ishimizu

Frontiers in Plant Science　Vol. 11　p. 997-997　2020/07/02　Research paper (scientific journal)

Rhamnogalacturonan I galactosyltransferase: Detection of enzyme activity and its hyperactivation.

Naoki Matsumoto, Yuto Takenaka, Bussarin Wachananawat, Hiroyuki Kajiura, Tomoya Imai, Takeshi Ishimizu

Plant physiology and biochemistry : PPB　Vol. 142　p. 173-178　2019/09　Research paper (scientific journal)

Practical preparation of UDP-apiose and its applications for studying apiosyltransferase.

Tae Fujimori, Ryoko Matsuda, Mami Suzuki, Yuto Takenaka, Hiroyuki Kajiura, Yoichi Takeda, Takeshi Ishimizu

Carbohydrate research　Vol. 477　p. 20-25　2019/05　Research paper (scientific journal)

Comparison of the N-glycosylation on recombinant miraculin expressed in tomato plants with native miraculin

Hiroyuki Kajiura, Kyoko Hiwasa-Tanase, Hiroshi Ezura, Kazuhito Fujiyama

Plant Biotechnology　Vol. 35　No. 4　p. 375-379　2018/12/25　Research paper (scientific journal)

Pectin RG-I rhamnosyltransferases represent a novel plant-specific glycosyltransferase family.

Yuto Takenaka, Kohei Kato, Mari Ogawa-Ohnishi, Kana Tsuruhama, Hiroyuki Kajiura, Kenta Yagyu, Atsushi Takeda, Yoichi Takeda, Tadashi Kunieda, Ikuko Hara-Nishimura, Takeshi Kuroha, Kazuhiko Nishitani, Yoshikatsu Matsubayashi, Takeshi Ishimizu

Nature plants　Vol. 4　No. 9　p. 669-676　2018/09　Research paper (scientific journal)

Degradation pathway of plant complex-type N -glycans: Identification and characterization of a key alpha1,3-fucosidase from Glycoside Hydrolase Family 29.

Kato S, Hayashi M, Kitagawa M, Kajiura H, Maeda M, Kimura Y, Igarashi K, Kasahara M, Ishimizu T

Biochem J.　Vol. 475　No. 1　p. 305-317-317　2018/04　Research paper (scientific journal)

Hydroponic treatment of Nicotiana benthamiana with kifunensine modifies the N-glycans of recombinant glycoprotein antigens to predominantly Man9 high-mannose type upon transient overexpression.

Roychowdhury S, Oh YJ, Kajiura H, Hamorsky KT, Fujiyama K, Matoba N

Front Plant Sci.　Vol. 9　p. 62-62　2018/04　Research paper (scientific journal)

A truncated form of human alpha 1-acid glycoprotein is useful as a molecular tool for insect glycobiology

Morokuma D, Hino M, Masuda A, Mon H, Fujiyama K, Kajiura H, Kusakabe T, Lee JM

Int J Indust. Entomol.　Vol. 36　No. 1　p. 15-24　2018/03

Gene coexpression network for trans-1,4-polyisoprene biosynthesis involving mevalonate and methylerythritol phosphate pathways in Eucommia ulmoides Oliver.

Tokumoto Y, Uefuji H, Yamamoto N, Kajiura H, Takeno S, Suzuki N, Nakazawa Y

Plant Biotechnol.　Vol. 34　No. 3　p. 165-172-172　2017/04　Research paper (scientific journal)

Fucosyltransferases produce N-glycans containing core l-galactose.

Ohashi H, Ohashi T, Kajiura H, Misaki R, Kitamura S, Fujiyama K

Biochem Biophys Res Commun.　Vol. 483　No. 1　p. 658-663-663　2017/04　Research paper (scientific journal)

N-glycan structures and downstream mannose-phosphorylation of plant recombinant human alpha-L-iduronidase: toward development of enzyme replacement therapy for mucopolysaccharidosis I.

Pierce OM, McNair GR, He X, Kajiura H, Fujiyama K, Kermode AR

Plant Mol Biol.　Vol. 95　No. 6　p. 593-606-606　2017/04　Research paper (scientific journal)

Induction of tetraploid hardy rubber tree, Eucommia ulmoides, and phenotypic differences from diploid.

Tokumoto Y, Kajiura H, Takeno S, Harada Y, Suzuki N, Hosaka T, Gyokusen K, Nakazawa Y

Plant Biotechnol.　Vol. 33　No. 1　p. 51-57-57　2016/04　Research paper (scientific journal)

Altered glycosylation of exported proteins,including surface immune receptors, compromises calcium and downstream signaling responses to microbe-associated molecular patterns in Arabidopsis thaliana.

Trempel F, Kajiura H, Ranf S, Grimmer J, Westphal L, Zipfel C, Scheel D, Fujiyama K, Lee J

BMC Plant Biol.　Vol. 16　p. 31-31　2016/04　Research paper (scientific journal)

Recombinant production and characterization of human anti-influenza virus monoclonal antibodies identified from hybridomas fused with human lymphocytes.

Misaki R, Fukura N, Kajiura H, Yasugi M, Kubota-Koketsu R, Sasaki T, Momota M, Ono K, Ohashi T, Ikuta K, Fujiyama K

Biologicals　Vol. 44　No. 5　p. 394-402-402　2016/04　Research paper (scientific journal)

N-glycosylation of cholera toxin B subunit in Nicotiana benthamiana: impacts on host stress response, production yield and vaccine potential.

Hamorsky KT, Kouokam JC, Jurkiewicz JM, Nelson B, Moore LJ, Husk AS, Kajiura H, Fujiyama K, Matoba N

Sci Rep.　Vol. 23　p. 5:8003-8003　2015/04　Research paper (scientific journal)

A watermarking system for labeling genomic DNA.

Yamamoto N, Kajiura H, Takeno S, Suzuki N, Nakazawa Y

Plant Biotechnol.　Vol. 31　No. 3　p. 241-248-U207　2014/04　Research paper (scientific journal)

Insight of Plant N-glycosylation Pathway through Heterologously—Expressed Enzymes

KAJIURA Hiroyuki, FUJIYAMA Kazuhito

KAGAKU TO SEIBUTSU　Vol. 52　No. 11　p. 719-724　2014

Rapid and scalable plant-based production of a cholera toxin B subunit variant to aid in mass vaccination against cholera outbreaks.

Hamorsky KT, Kouokam JC, Bennett LJ, Baldauf KJ, Kajiura H, Fujiyama K, Matoba N

PLoS Negl Trop Dis.　Vol. 7　No. 3　2013/04　Research paper (scientific journal)

Transgenic Rice Seeds Accumulating Recombinant Hypoallergenic Birch Pollen Allergen Bet v 1 Generate Giant Protein Bodies.

Wang S, Takahashi H, Kajiura H, Kawakatsu T, Fujiyama K, Takaiwa F

Plant Cell Physiol.　Vol. 54　No. 6　p. 917-933-933　2013/04　Research paper (scientific journal)

植物がつくるバイオポリマー；ポリイソプレン

梶浦 裕之

生物工学会誌　Vol. 91　No. 12　p. 720-720　2013

N-Glycan structures of glycoproteins in suspension-cultured Arabidopsis thaliana T87 cells.

Kajiura H, Tanaka R, Kato K, Hamaguchi Y, Seki T, Fujiyama K

Plant Biotechnol.　Vol. 29　No. 5　p. 489-493-493　2012/04　Research paper (scientific journal)

Enhancement of sialylation on humanized IgG-like bispecific antibody by overexpression of alpha2,6-sialyltransferase derived from Chinese hamster ovary cells.

Onitsuka M, Kim WD, Ozaki H, Kawaguchi A, Honda K, Kajiura H, Fujiyama K, Asano R, Kumagai I, Ohtake H, Omasa T

Appl Microbiol Biotechnol.　Vol. 94　No. 1　p. 69-80-80　2012/04　Research paper (scientific journal)

Production of different glycosylation variants of the tumour-targeting mAb H10 in Nicotiana benthamiana: influence on expression yield and antibody degradation.

Lombardi R, Donini M, Villani ME, Brunetti P, Fujiyama K, Kajiura H, Paul M, Ma JK, Benvenuto E

Transgenic Res.　Vol. 21　No. 5　p. 1005-1021-1021　2012/04　Research paper (scientific journal)

Evolutionarily conserved glycan signal to degrade aberrant brassinosteroid receptors in Arabidopsis.

Hong Z, Kajiura H, Su W, Jin H, Kimura A, Fujiyama K,Li J

Proc Natl Acad Sci U S A　Vol. 109　No. 28　p. 11437-11442-11442　2012/04　Research paper (scientific journal)

植物における糖鎖の制御

梶浦裕之, 三崎 亮, 藤山和仁

バイオ医薬品製造の効率化と生産基材の開発　2012

Reduced Immunogenicity of Arabidopsis hgl1 Mutant N-Glycans Caused by Altered Accessibility of Xylose and core Fucose Epitopes

Heidi Kaulfuerst-Soboll, Stephan Rips, Hisashi Koiwa, Hiroyuki Kajiura, Kazuhito Fujiyama, Antje von Schaewen

JOURNAL OF BIOLOGICAL CHEMISTRY　Vol. 286　No. 26　p. 22955-22964　2011/07　Research paper (scientific journal)

Reduced immunogenicity of Arabidopsis hybrid glycosylation1 (hgl1) N-glycans due to altered accessibility of xylose and core fucose epitopes.

Kaulfuerst-Soboll H, Rips S, Koiwa H, Kajiura H, Fujiyama K, von Schaewen A

J Biol Chem.　Vol. 286　2011/04　Research paper (scientific journal)

Rice Golgi proteome : identification and characterization of N-acetylglucosaminyltransferase-I-like protein.

Mitsui T, Asakura T, Nakayama Y, Takayama K, Hirose S, Katamine H, Aoyama M, Karahashi A, Kaneko K, Ishiyama R, Itoh K, Hori H, Kajiura H, Fujiyama K

Bull. Facul. Agric. Niigata Univ.　Vol. 63　2010/04　Research paper (scientific journal)

Glycosylation pattern of humanized IgG-like bispecific antibody produced by recombinant CHO cells.

Kim WD, Tokunaga M, Ozaki H, Ishibashi T, Honda K, Kajiura H, Fujiyama K, Asano R, Kumagai I, Omasa T, Ohtake H

Appl Microbiol Biotechnol.　Vol. 85　No. 3　p. 535-542-542　2010/04　Research paper (scientific journal)

The impact of the overexpression of human UDP-galactose transporter gene hUGT1 in tobacco plants.

Khalil MF, Kajiura H, Fujiyama K, Koike K, Ishida N, Tanaka N

J Biosci Bioeng.　Vol. 109　No. 2　p. 159-169-169　2010/04　Research paper (scientific journal)

Biochemical and immunological characterization of the plant-derived candidate human immunodeficiency virus type 1 mucosal vaccine CTB-MPR.

Matoba N, Kajiura H, Cherni I, Doran JD, Bomsel M, Fujiyama K, Mor TS

Plant Biotechnol J.　Vol. 7　No. 2　p. 129-145-145　2009/04　Research paper (scientific journal)

Cloning and characterization of cytidine monophosphate-3-deoxy-D-manno-octulosonate synthetase from Arabidopsis thaliana.

Misaki R, Kajiura H, Fujii K, Fujiyama K, Seki T

J Biosci Bioeng.　Vol. 108　No. 6　p. 527-529-529　2009/04　Research paper (scientific journal)

Generation of a transgenic rice seed-based edible vaccine against house dust mite allergy.

Yang L, Kajiura H, Suzuki K, Hirose S, Fujiyama K, Takaiwa F

Biochem Biophys Res Commun.　Vol. 365　No. 2　p. 334-339-339　2008/04　Research paper (scientific journal)

Salt tolerance of Arabidopsis thaliana requires maturation of N-glycosylated proteins in the Golgi apparatus.

Kang JS, Frank J, Kang CH, Kajiura H, Vikram M, Ueda A, Kim S, Bahk JD, Triplett B, Fujiyama K, Lee SY, von, Schaewen A, Koiwa H

Proc Natl Acad Sci U S A　Vol. 105　No. 15　p. 5933-5938-5938　2008/04　Research paper (scientific journal)

Salt tolerance of Arabidopsis thaliana requires maturation of N-glycosylated proteins in the Golgi apparatus (Proceedings of the National Academy of Sciences of the United States of America (2008) 105, 15 (5933-5938) DOI: 10.1073/pnas.0800237105)

Kang, J.S., Frank, J., Kang, C.H., Kajiura, H., Vikram, M., Ueda, A., Kim, S., Bahk, J.D., Triplett, B., Fujiyama, K., Lee, S.Y., Von Schaewen, A., Koiwa, H.

Proceedings of the National Academy of Sciences of the United States of America　Vol. 105　No. 22　p. 7893-7893　2008　Research paper (scientific journal)

Evidence for new beta1-3 galactosyltransferase activity involved in biosynthesis of unusual N-glycan harboring T-antigen in Apis mellifera.

Kimura Y, Sakamura S, Ushijima T, Hama Y, Kajiura H, Fujiyama K, Okihara K, Hashimoto K, Sugimoto H, Yamada H

Biosci Biotechnol Biochem.　Vol. 71　No. 4　p. 1111-1114-1114　2007/04　Research paper (scientific journal)