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Versatile confocal microscopy for imaging objects oscillating at frequencies exceeding the frame rate
Kanta Adachi, Seita Mukai, Kenichi Higami, Takumi Saito, Nobutomo Nakamura
Applied Physics Letters 2025/06/30 Research paper (scientific journal)
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Mechanisms of force transmission on cytokinesis Formin Cdc12 in fission yeast revealed by coiled-coil force sensors
Takumi Saito, Yuan Ren, Julien Berro
2025/05/14 Research paper (scientific journal)
Publisher: Cold Spring Harbor Laboratory
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Mapping molecular diffusion across the whole cell with spatial statistics-based FRAP
Yohei Okabe, Takumi Saito, Outa Nakashima, Daiki Matsunaga, Shinji Deguchi
2025/02/08 Research paper (scientific journal)
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Decoupling diffusion, turnover, and advection in long-term FRAP
Outa Nakashima, Takumi Saito, Shinji Deguchi
2025/02/04 Research paper (scientific journal)
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Genetically encoded mechano-sensors with versatile readouts and compact size
Yuan Ren, Jie Yang, Takumi Saito, Oliver Glomb, Avinash Kumar, Sayed Iman Mousavi, Brigitte Naughton, Christina de Fontnouvelle, Barbara Fujita, Zhiqun Xi, Christian Schlieker, Shaul Yogev, Yongli Zhang, Julien Berro
2025/01/18 Research paper (scientific journal)
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Fast Fission Yeast Genome Editing by CRISPR/Cas9 Using Gap Repair and Fluoride Selection
Yuan Ren, Ronan Fernandez, Takumi Saito, Barbara Fujita, Iman Mousavi, Julien Berro
Methods in Molecular Biology p. 141-154 2024/11/12 Part of collection (book)
Publisher: Springer US
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Intracellular Macromolecular Crowding within Individual Stress Fibers Analyzed by Fluorescence Correlation Spectroscopy
Aria Buenaventura, Takumi Saito, Taiga Kanao, Daiki Matsunaga, Tsubasa S. Matsui, Shinji Deguchi
Cellular and Molecular Bioengineering 2024/06/07 Research paper (scientific journal)
Publisher: Springer Science and Business Media LLC
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Glucose stockpile in the intestinal apical brush border in C. elegans
Saito, T., Kikuchi, K., Ishikawa, T.
Biochemical and Biophysical Research Communications Vol. 706 2024/04 Research paper (scientific journal)
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Advancing FRAP for cell studies: Where there is a new method, there is a new field
Takumi SAITO, Shinji DEGUCHI
Journal of Biomechanical Science and Engineering Vol. 18 No. 4 p. 23-00028 2023 Research paper (scientific journal)
Publisher: Japan Society of Mechanical Engineers
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CM‐FRAP–Continuum Mechanics‐Based Fluorescence Recovery After Photobleaching
Takumi Saito, Shinji Deguchi
Current Protocols Vol. 3 No. 1 2023/01 Research paper (scientific journal)
Publisher: Wiley
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Long-Term Fluorescence Recovery After Photobleaching (FRAP)
Takumi Saito, Daiki Matsunaga, Shinji Deguchi
Methods in Molecular Biology p. 311-322 2023 Research paper (scientific journal)
Publisher: Springer {US}
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Long-term molecular turnover of actin stress fibers revealed by advection-reaction analysis in fluorescence recovery after photobleaching
Takumi Saito, Kun Chen, Daiki Matsunaga, Shinji Deguchi
PLOS ONE Vol. 17 No. 11 p. e0276909-e0276909 2022/11/07 Research paper (scientific journal)
Publisher: Cold Spring Harbor Laboratory
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Analysis of chemomechanical behavior of stress fibers by continuum mechanics-based FRAP.
Takumi Saito, Daiki Matsunaga, Shinji Deguchi
Biophysical journal Vol. 121 No. 15 p. 2921-2930 2022/08/02 Research paper (scientific journal)
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Analysis of chemical and mechanical behaviors in living cells by continuum mechanics-based FRAP
Takumi Saito, Daiki Matsunaga, Shinji Deguchi
2022/04/16 Research paper (scientific journal)
Publisher: Cold Spring Harbor Laboratory
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Determining the domain-level reaction-diffusion properties of an actin-binding protein transgelin-2 within cells.
Takumi Saito, Daiki Matsunaga, Tsubasa S Matsui, Kentaro Noi, Shinji Deguchi
Experimental cell research Vol. 404 No. 1 p. 112619-112619 2021/07/01 Research paper (scientific journal)
Publisher: Elsevier
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Mechanosensitive myosin II but not cofilin primarily contributes to cyclic cell stretch-induced selective disassembly of actin stress fibers.
Wenjing Huang, Tsubasa S Matsui, Takumi Saito, Masahiro Kuragano, Masayuki Takahashi, Tomohiro Kawahara, Masaaki Sato, Shinji Deguchi
American journal of physiology. Cell physiology Vol. 320 No. 6 p. C1153-C1163-C1163 2021/06/01 Research paper (scientific journal)
Publisher: American Physiological Society Rockville, MD
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What factors determine the number of nonmuscle myosin II in the sarcomeric unit of stress fibers?
Takumi Saito, Wenjing Huang, Tsubasa S Matsui, Masahiro Kuragano, Masayuki Takahashi, Shinji Deguchi
Biomechanics and modeling in mechanobiology Vol. 20 No. 1 p. 155-166 2021/02 Research paper (scientific journal)
Publisher: Springer
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Using FCS in the analysis of macromolecular crowding effects of stress fibers on GFP diffusion
Buenaventura, Aria Buenaventura Aria, Saito, Takumi, Kanao, Taiga, Matsunaga, Daiki, Deguchi, Shinji
Transactions of Japanese Society for Medical and Biological Engineering No. Abstract p. 479-479 2021 Research paper (scientific journal)
Publisher: Japanese Society for Medical and Biological Engineering