Evaluating the effect of two-dimensional molecular layout on DNA origami-based transporters

Kodai Fukumoto, Yuya Miyazono, Takuya Ueda, Yoshie Harada, Hisashi Tadakuma

Nanoscale Advances　Vol. 5　No. 9　p. 2590-2601　2023/04/19　Research paper (scientific journal)

Highly Dispersed 3C Silicon Carbide Nanoparticles with a Polydopamine/Polyglycerol Shell for Versatile Functionalization

Shingo Sotoma, Hiroshi Abe, Yohei Miyanoiri, Takeshi Ohshima, Yoshie Harada

ACS Applied Materials & Interfaces　Vol. 15　p. 21413-21424　2023/04/18　Research paper (scientific journal)

Nanaomycin E inhibits NLRP3 inflammasome activation by preventing mitochondrial dysfunction

Yudai Matsui, Naoki Takemura, Yoshitaka Shirasaki, Michihiro Takahama, Yoshihiko Noguchi, Kenta Ikoma, Yixi Pan, Shuhei Nishida, Manabu Taura, Akiyoshi Nakayama, Takashi Funatsu, Takuma Misawa, Yoshie Harada, Toshiaki Sunazuka, Tatsuya Saitoh

International Immunology　Vol. 34　No. 10　p. 505-518　2022/09/09　Research paper (scientific journal)

Heat-hypersensitive mutants of ryanodine receptor type 1 revealed by microscopic heating

Kotaro Oyama, Vadim Zeeb, Toshiko Yamazawa, Nagomi Kurebayashi, Fuyu Kobirumaki-Shimozawa, Takashi Murayama, Hideto Oyamada, Satoru Noguchi, Takayoshi Inoue, Yukiko U. Inoue, Ichizo Nishino, Yoshie Harada, Norio Fukuda, Shin’ichi Ishiwata, Madoka Suzuki

Proceedings of the National Academy of Sciences　Vol. 119　No. 32　2022/08/09　Research paper (scientific journal)

Modulation of Local Cellular Activities using a Photothermal Dye-Based Subcellular-Sized Heat Spot

Ferdinandus, Madoka Suzuki, Cong Quang Vu, Yoshie Harada, Satya Ranjan Sarker, Shin ichi Ishiwata, Tetsuya Kitaguchi, Satoshi Arai

ACS Nano　Vol. 16　No. 6　p. 9004-9018　2022/06/08　Research paper (scientific journal)

Cell-autonomous control of intracellular temperature by unsaturation of phospholipid acyl chains

Akira Murakami, Kohjiro Nagao, Reiko Sakaguchi, Keisuke Kida, Yuji Hara, Yasuo Mori, Kohki Okabe, Yoshie Harada, Masato Umeda

Cell Reports　Vol. 38　No. 11　p. 110487-110487　2022/03　Research paper (scientific journal)

Quantum nanodiamonds for sensing of biological quantities: Angle, temperature, and thermal conductivity

Shingo Sotoma, Hirotaka Okita, Shunsuke Chuma, Yoshie Harada

Biophysics and Physicobiology　Vol. 19　p. n/a-n/a　2022

Photothermal Dye-based Subcellular-sized Heat Spot Enabling the Modulation of Local Cellular Activities

Ferdinandus Ferdinandus, Madoka Suzuki, Yoshie Harada, Satya Ranjan Sarker, Shin ichi Ishiwata, Tetsuya Kitaguchi, Satoshi Arai

bioRxiv　2021/12/21

Pressure-induced changes on the morphology and gene expression in mammalian cells

Kazuko Okamoto, Tomonobu M. Watanabe, Masanobu Horie, Masayoshi Nishiyama, Yoshie Harada, Hideaki Fujita

Biology Open　Vol. 10　No. 7　2021/07/15　Research paper (scientific journal)

細胞の熱伝導率を定量する―量子センサーによるナノバイオセンシング

外間進悟, 鈴木団, 原田慶恵

化学　Vol. 76　No. 5　p. 32-36　2021/05

Composite Quantum Sensors Based on Fluorescent Nanodiamonds for Intracellular Controlled Heating in Living Cells

Shingo Sotoma, Yoshie Harada

ACS Applied Nano Materials　Vol. 4　No. 4　p. 3969-3976　2021/04/23　Research paper (scientific journal)

In situ measurements of intracellular thermal conductivity using heater-thermometer hybrid diamond nanosensors

Shingo Sotoma, Chongxia Zhong, James Chen Yong Kah, Hayato Yamashita, Taras Plakhotnik, Yoshie Harada, Madoka Suzuki

Science Advances　Vol. 7　No. 3　p. eabd7888-eabd7888　2021/01　Research paper (scientific journal)

Viral RNA recognition by LGP2 and MDA5, and activation of signaling through step-by-step conformational changes

Ivana Duic, Hisashi Tadakuma, Yoshie Harada, Ryo Yamaue, Deguchi Katashi, Yuki Suzuki, Shige Yoshimura, Hiroki Kato, Kunio Takeyasu, Takashi Fujita

Nucleic acids research　Vol. 48　No. 20　p. 11664-11674　2020/11　Research paper (scientific journal)

Heat hypersensitivity of ryanodine receptor type 1 mutants implicated in malignant hyperthermia

Kotaro Oyama, Vadim Zeeb, Toshiko Yamazawa, Takashi Murayama, Hideto Oyamada, Yoshie Harada, Norio Fukuda, Shin’ichi Ishiwata, Madoka Suzuki

bioRxiv　2020/10/29　Research paper (scientific journal)

量子センサーを用いた生細胞観察

外間進悟, 原田慶恵

光学　Vol. 49　No. 8　p. 311-316　2020/08　Research paper (scientific journal)

In situ measurement of intracellular thermal conductivity using heater-thermometer hybrid diamond nanosensor

Shingo Sotoma, Chongxia Zhong, James Chen Yong Kah, Hayato Yamashita, Taras Plakhotnik, Yoshie Harada, Madoka Suzuki

bioRxiv　2020/06/04　Research paper (scientific journal)

Tracking the 3D Rotational Dynamics in Nanoscopic Biological Systems

Ryuji Igarashi, Takuma Sugi, Shingo Sotoma, Takuya Genjo, Yuta Kumiya, Erik Walinda, Hiroshi Ueno, Kazuhiro Ikeda, Hitoshi Sumiya, Hidehito Tochio, Yohsuke Yoshinari, Yoshie Harada, Masahiro Shirakawa

Journal of the American Chemical Society　Vol. 142　No. 16　p. 7542-7554　2020/04/22　Research paper (scientific journal)

蛍光ダイヤモンド粒子を用いた生体イメージング

原田慶恵

ATI News　No. 27　p. 2-7　2019/10

Formation of perfectly aligned high-density NV centers in (111) CVD-grown diamonds for magnetic field imaging of magnetic particles

Hayato Ozawa, Yuji Hatano, Takayuki Iwasaki, Yoshie Harada, Mutsuko Hatano

Japanese Journal of Applied Physics　Vol. 58　No. SI　p. SIIB26-SIIB26　2019/07/17　Research paper (scientific journal)

Introduction to the Biophysical Society of Japan (BSJ).

Hideki Kandori, Yoshie Harada, Hiroyuki Noji

Biophysical reviews　Vol. 11　No. 3　p. 265-266　2019/06

Polydopamine Coating as a Scaffold for Ring-Opening Chemistry To Functionalize Gold Nanoparticles

Shingo Sotoma, Yoshie Harada

Langmuir　Vol. 35　No. 25　p. 8357-8362　2019/06　Research paper (scientific journal)

HCV IRES Captures an Actively Translating 80S Ribosome

Takeshi Yokoyama, Kodai Machida, Wakana Iwasaki, Tomoaki Shigeta, Madoka Nishimoto, Mari Takahashi, Ayako Sakamoto, Mayumi Yonemochi, Yoshie Harada, Hideki Shigematsu, Mikako Shirouzu, Hisashi Tadakuma, Hiroaki Imataka, Takuhiro Ito

Molecular Cell　Vol. 74　No. 6　p. 1205-1214.e8　2019/06　Research paper (scientific journal)

Multifunctional temozolomide-loaded lipid superparamagnetic nanovectors: dual targeting and disintegration of glioblastoma spheroids by synergic chemotherapy and hyperthermia treatment

Attilio Marino, Alice Camponovo, Andrea Degl'Innocenti, Martina Bartolucci, Christos Tapeinos, Chiara Martinelli, Daniele De Pasquale, Francesca Santoro, Valentina Mollo, Satoshi Arai, Madoka Suzuki, Yoshie Harada, Andrea Petretto, Gianni Ciofani

Nanoscale　Vol. 11　No. 44　p. 21227-21248　2019　Research paper (scientific journal)

ダイヤモンドセンサーデバイスとシステム構築

波多野雄治, 小澤勇斗, 岩崎孝之, 波多野睦子, 安田晋, 大島武, 原田慶恵

NEW DIAMOND　Vol. 35　No. 1　p. 7-13　2019/01

ダイヤモンド

原田慶恵

実験医学 増刷　Vol. 36　No. 20　p. 171-173　2018/12

Enrichment of ODMR-active nitrogen-vacancy centres in five-nanometre-sized detonation-synthesized nanodiamonds: Nanoprobes for temperature, angle and position

Shingo Sotoma, Daiki Terada, Takuya F. Segawa, Ryuji Igarashi, Yoshie Harada, Masahiro Shirakawa

Scientific Reports　Vol. 8　No. 1　2018/12/01　Research paper (scientific journal)

Magnetic Field Imaging of Superparamagnetic Particles Using High-Density, Perfectly Oriented NV Centers in Diamond CVD Film

Yuji Hatano, Takeharu Sekiguchi, Takayuki Iwasaki, Mutsuko Hatano, Yoshie Harada

physica status solidi (a)　Vol. 215　No. 22　p. 1800254-1800254　2018/11　Research paper (scientific journal)

Fluorescent Nanodiamonds as a Robust Temperature Sensor inside a Single Cell

Takeharu Sekiguchi, Shingo Sotoma, Yoshie Harada

Biophysics and Physicobiology　Vol. 15　p. 229-234　2018/10　Research paper (scientific journal)

Construction of integrated gene logic-chip

Takeya Masubuchi, Masayuki Endo, Ryo Iizuka, Ayaka Iguchi, Dong Hyun Yoon, Tetsushi Sekiguchi, Hao Qi, Ryosuke Iinuma, Yuya Miyazono, Shuichi Shoji, Takashi Funatsu, Hiroshi Sugiyama, Yoshie Harada, Takuya Ueda, Hisashi Tadakuma

Nature Nanotechnology　Vol. 13　No. 10　p. 933-940　2018/10/01　Research paper (scientific journal)

One-Pot Synthesis of Highly Dispersible Fluorescent Nanodiamonds for Bioconjugation.

Daiki Terada, Shingo Sotoma, Yoshie Harada, Ryuji Igarashi, Masahiro Shirakawa

Bioconjug. Chem.　Vol. 29　No. 8　p. 2786-2792　2018/08　Research paper (scientific journal)

Commonly stabilized cytochromes c from deep-sea Shewanella and Pseudomonas.

Sotaro Fujii, Misa Masanari-Fujii, Shinya Kobayashi, Chiaki Kato, Masayoshi Nishiyama, Yoshie Harada, Satoshi Wakai, Yoshihiro Sambongi

Bioscience, biotechnology, and biochemistry　p. 1-8　2018/03/14　Research paper (scientific journal)

Why Do We Try to Image the Temperature Changes in Individual Brown Adipocytes?

Madoka Suzuki, Yoshie Harada

Seibutsu Butsuri　Vol. 58　No. 2　p. 97-99　2018/03

Pressure-Ineduced Activation of the Swimming Motility of Magnetotactic Bacterium

Masayoshi Nishiyama, Ruan Juanfang, Takayuki Kato, Toru Minamino, Keiichi Namba, Akitoshi Seiyama, Long-Fei Wu, Yoshie Harada

Biophysical Journal　Vol. 114　No. 3　p. 324a-324a　2018/02　Research paper (scientific journal)

Morphological Control of Microtubule-Encapsulating Giant Vesicles by Changing Hydrostatic Pressure.

Kingo Takiguchi, Masahito Hayashi, Yuki Kazayama, Taro Toyota, Yoshie Harada, Masayoshi Nishiyama

Biological & pharmaceutical bulletin　Vol. 41　No. 3　p. 288-293　2018　Research paper (scientific journal)

DNAオリガミを用いた直行性を有する転写デバイスの合理的設計

増渕 岳也, 遠藤 政幸, 飯塚 怜, 井口 彩香, Yoon Dong, 関口 哲志, Qi Hao, 飯沼 良介, 宮薗 侑也, 庄子 習一, 船津 高志, 杉山 弘, 原田 慶恵, 上田 卓也, 多田隈 尚史

生命科学系学会合同年次大会　Vol. 2017年度　p. [2P-1323]　2017/12

Live Cell Imaging Using Photoswitchable Diarylethene-Doped Fluorescent Polymer Dots

Yasuko Osakada, Tuyoshi Fukaminato, Yuma Ichinose, Mamoru Fujitsuka, Yoshie Harada, Tetsuro Majima

CHEMISTRY-AN ASIAN JOURNAL　Vol. 12　No. 20　p. 2660-2665　2017/10　Research paper (scientific journal)

科学を愛するあなたのための研究費があります。基礎研究を追求する日本発の国際グラントHFSP フェローシップ獲得編

原田慶恵

実験医学　Vol. 35　No. 11　p. 1892-1897　2017/07

ダイヤモンド磁気センサの高感度化技術

波多野睦子, 岩崎孝之, 田原康佐, 牧野俊, 水落憲和, 波多野雄治, 原田慶恵, 安田晋

NEW DIAMOND　Vol. 33　No. 2　p. 7-13　2017/04

Preferential 5-Methylcytosine Oxidation in the Linker Region of Reconstituted Positioned Nucleosomes by Tet1 Protein

Seiichiro Kizaki, Tingting Zou, Yue Li, Yong-Woon Han, Yuki Suzuki, Yoshie Harada, Hiroshi Sugiyama

CHEMISTRY-A EUROPEAN JOURNAL　Vol. 22　No. 46　p. 16598-16601　2016/11　Research paper (scientific journal)

温度生物学の創成(展開を目指して) 細胞内温度の計測と操作による温度生物学

岡部 弘基, シ・ベイニ, 船津 高志, 原田 慶恵

日本細胞生物学会大会講演要旨集　Vol. 68回　p. 12-12　2016/05

Reversible Morphological Control of Tubulin-Encapsulating Giant Liposomes by Hydrostatic Pressure

Masahito Hayashi, Masayoshi Nishiyama, Yuki Kazayama, Taro Toyota, Yoshie Harada, Kingo Takiguchi

LANGMUIR　Vol. 32　No. 15　p. 3794-3802　2016/04　Research paper (scientific journal)

The application of fluorescence-conjugated pyrrole/imidazole polyamides in the characterization of protein-DNA complex formation

Yong-Woon Han, Hiroshi Sugiyama, Yoshie Harada

BIOMATERIALS SCIENCE　Vol. 4　No. 3　p. 391-399　2016　Research paper (scientific journal)

DNA折り紙上の異なった方式による遺伝子転写ナノデバイスの理論的な設計(Rational design of orthogonal gene transcription nano device on DNA origami)

増渕 岳也, 多田隈 向史, 飯塚 怜, 遠藤 政幸, 船津 高志, 杉山 弘, 原田 慶恵, 上田 卓也

日本生化学会大会・日本分子生物学会年会合同大会講演要旨集　Vol. 88回・38回　p. [3LBA089]-[3LBA089]　2015/12

Synergistic effect of ATP for RuvA-RuvB-Holliday junction DNA complex formation

Takuma Iwasa, Yong-Woon Han, Ryo Hiramatsu, Hiroaki Yokota, Kimiko Nakao, Ryuji Yokokawa, Teruo Ono, Yoshie Harada

SCIENTIFIC REPORTS　Vol. 5　2015/12　Research paper (scientific journal)

ECHO-liveFISH: in vivo RNA labeling reveals dynamic regulation of nuclear RNA foci in living tissues

Ikumi Oomoto, Asuka Suzuki-Hirano, Hiroki Umeshima, Yong-Woon Han, Hiroyuki Yanagisawa, Peter Carlton, Yoshie Harada, Mineko Kengaku, Akimitsu Okamoto, Tomomi Shimogori, Dan Ohtan Wang

NUCLEIC ACIDS RESEARCH　Vol. 43　No. 19　2015/10　Research paper (scientific journal)

科学を愛するあなたのための研究費があります。基礎研究を追求する日本発の国際グラントHFSP フェローシップ申請編

原田慶恵

実験医学　Vol. 33　No. 11　p. 1817-1823　2015/07

Suppression of Nonspecific Protein-Nanodiamond Adsorption Enabling Specific Targeting of Nanodiamonds to Biomolecules of Interest

Shingo Sotoma, Ryuji Igarashi, Jun Iimura, Yuta Kumiya, Hidehito Tochio, Yoshie Harada, Masahiro Shirakawa

CHEMISTRY LETTERS　Vol. 44　No. 3　p. 354-356　2015/03　Research paper (scientific journal)

Optically Detected Magnetic Resonance of Nanodiamonds In Vivo; Implementation of Selective Imaging and Fast Sampling

Yosuke Yoshinari, Shigeyuki Mori, Ryuji Igarashi, Takuma Sugi, Hiroaki Yokota, Kazuhiro Ikeda, Hitoshi Sumiya, Ikue Mori, Hidehito Tochio, Yoshie Harada, Masahiro Shirakawa

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY　Vol. 15　No. 2　p. 1014-1021　2015/02　Research paper (scientific journal)

Application of fluorescent diamond nanoparticles to bio-imaging

Ryuji Igarashi, Shingo Sotoma, Masahiro Shirakawa, Yoshie Harada

Optics InfoBase Conference Papers　Vol. 2019　2015　Research paper (international conference proceedings)

Comprehensive and quantitative analysis for controlling the physical/chemical states and particle properties of nanodiamonds for biological applications

S. Sotoma, K. Akagi, S. Hosokawa, R. Igarashi, H. Tochio, Y. Harada, M. Shirakawa

RSC ADVANCES　Vol. 5　No. 18　p. 13818-13827　2015　Research paper (scientific journal)

蛍光ダイヤモンド粒子を用いた生体イメージング技術の開発

原田慶恵

応用物理　Vol. 84　No. 1　p. 56-60　2015/01

Effective production of fluorescent nanodiamonds containing negatively-charged nitrogen-vacancy centers by ion irradiation

Shingo Sotoma, Yohsuke Yoshinari, Ryuji Igarashi, Akiyoshi Yamazaki, Shige H. Yoshimura, Hidehito Tochio, Masahiro Shirakawa, Yoshie Harada

DIAMOND AND RELATED MATERIALS　Vol. 49　p. 33-38　2014/10　Research paper (scientific journal)

Quantitative In Vivo Fluorescence Cross-Correlation Analyses Highlight the Importance of Competitive Effects in the Regulation of Protein-Protein Interactions

Wakako Sadaie, Yoshie Harada, Michiyuki Matsuda, Kazuhiro Aoki

MOLECULAR AND CELLULAR BIOLOGY　Vol. 34　No. 17　p. 3272-3290　2014/09　Research paper (scientific journal)

Characterization of the hemi-methylated CpG methylation process using fluorescent labeled SRA

Cui Yubing, Han Yong-Woon, Ariyoshi Mariko, Arita Kyohei, Suetake Isao, Tajima Shoji, Harada Yoshie

生物物理　Vol. 54　No. Suppl.1-2　p. S268-S268　2014/08

ナノ開口基板を用いたDNAメチル化パターン維持機構に関与する生体分子の機能解析

韓 龍雲, 原田 慶恵

生体の科学　Vol. 65　No. 6　p. 553-558　2014/06

ナノダイヤモンドNVCを使った新しい生体・細胞計測

杤尾豪人, 外間進悟, 原田慶恵

生化学　Vol. 86　No. 2　p. 145-153　2014/04

Real-time single-cell imaging of protein secretion

Yoshitaka Shirasaki, Mai Yamagishi, Nobutake Suzuki, Kazushi Izawa, Asahi Nakahara, Jun Mizuno, Shuichi Shoji, Toshio Heike, Yoshie Harada, Ryuta Nishikomori, Osamu Ohara

SCIENTIFIC REPORTS　Vol. 4　No. 4　2014/04　Research paper (scientific journal)

1P316 Rational design of orthogonal gene transcription nano device on DNA origami(28. Bioengineering,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

Masubuchi Takeya, Tadakuma Hisashi, Endo Masayuki, Sugiyama Hiroshi, Harada Yoshie, Ueda Takuya

Seibutsu Butsuri　Vol. 54　No. 1　2014

1P306 Analysis of non-classical secretion of IL-1β using real-time single-cell secretion imaging(27. Bioimaging,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

Shirasaki Yoshitaka, Liu Ting, Yamaguchi Yoshifumi, Yamaguchi Mai, Suzuki Nobutake, Izawa Kazushi, Mizuno Jun, Shoji Shuichi, Harada Yoshie, Nishikomori Ryuta, Heike Toshio, Miura Masayuki, Ohara Osamu

Seibutsu Butsuri　Vol. 54　No. 1　2014

3P272 Observing the rotational diffusion of nanodiamonds with arbitrary nitrogen vacancy center configurations(24. Mathematical biology,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

Kalay Ziya, Yoshinari Yohsuke, Harada Yoshie

Seibutsu Butsuri　Vol. 54　No. 1　2014

2P118 Single-molecule direct visualization of DNA binding modes of the mammalian nucleotide excision repair protein XPC(04. Nucleic acid binding proteins,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

Yokota Hiroaki, Tone Daisuke, Han Yong-Woon, Harada Yoshie, Sugasawa Kaoru

Seibutsu Butsuri　Vol. 54　No. 1　2014

Optically detected magnetic resonance of nanodiamonds in vivo.

Yoshie Harada

Lecture Notes on Cell Biology and Materials Science　2014/01

Construction and characterization of Cy3-or Cy5-conjugated hairpin pyrrole-imidazole polyamides binding to DNA in the nucleosome

Yong-Woon Han, Yasuo Tsunaka, Hiroaki Yokota, Tomoko Matsumoto, Gengo Kashiwazaki, Hironobu Morinaga, Kaori Hashiya, Toshikazu Bando, Hiroshi Sugiyama, Yoshie Harada

BIOMATERIALS SCIENCE　Vol. 2　No. 3　p. 297-307　2014　Research paper (scientific journal)

Hard X-ray-induced optical luminescence via biomolecule-directed metal clusters

Yasuko Osakada, Guillem Pratx, Conroy Sun, Masanori Sakamoto, Moiz Ahmad, Olga Volotskova, Qunxiang Ong, Toshiharu Teranishi, Yoshie Harada, Lei Xing, Bianxiao Cui

CHEMICAL COMMUNICATIONS　Vol. 50　No. 27　p. 3549-3551　2014　Research paper (scientific journal)

Observing the rotational diffusion of nanodiamonds with arbitrary nitrogen vacancy center configurations

Yohsuke Yoshinari, Ziya Kalay, Yoshie Harada

PHYSICAL REVIEW B　Vol. 88　No. 23　2013/12　Research paper (scientific journal)

SCJ Public Symposium on Integrative Bio-imaging_Initiative for the Revolution of Medical and Life Sciences

SOKABE Masahiro, NAGAYAMA Kuniaki, NAMBA Keiichi, HARADA Yoshie

Seibutsu Butsuri　Vol. 53　No. 6　p. 329-333　2013/11/25

Effect of single pyrrole replacement with β-alanine on DNA binding affinity and sequence specificity of hairpin pyrrole/imidazole polyamides targeting 5′-GCGC-3′

Yong-Woon Han, Gengo Kashiwazaki, Hironobu Morinaga, Tomoko Matsumoto, Kaori Hashiya, Toshikazu Bando, Yoshie Harada, Hiroshi Sugiyama

Bioorganic and Medicinal Chemistry　Vol. 21　No. 17　p. 5436-5441　2013/09/01　Research paper (scientific journal)

ダイヤモンドナノ粒子を使った新しい生体分子蛍光イメージング法の開発

原田 慶恵

現代化学 東京化学同人　Vol. 511　p. 42-46　2013/09

In Vivo Fluorescent Adenosine 5'-Triphosphate (ATP) Imaging of Drosophila melanogaster and Caenorhabditis elegans by Using a Genetically Encoded Fluorescent ATP Biosensor Optimized for Low Temperatures

Taiichi Tsuyama, Jun-ichi Kishikawa, Yong-Woon Han, Yoshie Harada, Asako Tsubouchi, Hiroyuki Noji, Akira Kakizuka, Ken Yokoyama, Tadashi Uemura, Hiromi Imamura

ANALYTICAL CHEMISTRY　Vol. 85　No. 16　p. 7889-7896　2013/08　Research paper (scientific journal)

ParA-mediated plasmid partition driven by protein pattern self-organization

Ling Chin Hwang, Anthony G. Vecchiarelli, Yong-Woon Han, Michiyo Mizuuchi, Yoshie Harada, Barbara E. Funnell, Kiyoshi Mizuuchi

EMBO Journal　Vol. 32　No. 9　p. 1238-1249　2013/05/02　Research paper (scientific journal)

Single-Molecule Imaging of the Oligomer Formation of the Nonhexameric Escherichia coli UvrD Helicase

Hiroaki Yokota, Yuko Ayabe Chujo, Yoshie Harada

BIOPHYSICAL JOURNAL　Vol. 104　No. 4　p. 924-933　2013/02　Research paper (scientific journal)

Development of a Novel Single-molecule Imaging Technique Using Fluorescent Diamond Nanoparticles

IGARASHI Ryuji, YOSHINARI Yohsuke, YOKOTA Hiroaki, SHIRAKAWA Masahiro, HARADA Yoshie

電気学会研究会資料. OQD, 光・量子デバイス研究会　Vol. 2013　No. 1　p. 1-3　2013/01/22

1P301 The localization of actin-related mRNAs in growth cone at a single molecule level(27. Bioimaging,Poster)

Koizumi Hidenori, Osakada Yasuko, Harada Yoshie

Seibutsu Butsuri　Vol. 53　No. 1　2013

1P300 X-ray excited optical luminescence via bio-molecule directed metal clusters(27. Bioimaging,Poster)

Osakada Yasuko, Harada Yoshie

Seibutsu Butsuri　Vol. 53　No. 1　2013

2P117 Single-molecule direct visualization of the mammalian nucleotide excision repair protein XPC(04. Nucleic acid binding proteins,Poster)

Yokota Hiroaki, Tone Daisuke, Han Yong-Woon, Harada Yoshie, Sugasawa Kaoru

Seibutsu Butsuri　Vol. 53　No. 1　2013

2P119 Single-molecule visualization of RuvB oligomer for characterizing a AAA^+ class hexameric ATPase with zero-mode waveguides(04. Nucleic acid binding proteins,Poster)

Iwasa Takuma, Han Yong-Woon, Yokota Hiroaki, Yokokawa Ryuji, Hiramatsu Ryo, Ono Teruo, Harada Yoshie

Seibutsu Butsuri　Vol. 53　No. 1　2013

1SCP-05 Optically detected magnetic resonance spectroscopy of nitrogen-vacancy centers for subnanoscopic measurement in vivo(1SCP Challenges to in vivo biophysics,Symposium,The 51th Annual Meeting of the Biophysical Society of Japan)

Igarashi Ryuji, Kumiya Yuta, Sugi Takuma, Sugihara Fuminori, Tochio Hidehito, Yoshinari Yousuke, Harada Yoshie, Shirakawa Masahiro

Seibutsu Butsuri　Vol. 53　No. 1　2013

3P306 Construction and functional analysis of DNA origami base DNA-RNAP hybrid nanomachine(28.Bioengineering,Poster,The 51st Annual Meeting of the Biophysical Society of Japan)

Masubuchi Takeya, Tadakuma Hisashi, Endo Masayuki, Sugiyama Hiroshi, Harada Yoshie, Ueda Takuya

Seibutsu Butsuri　Vol. 53　No. 1　2013

3P283 Optically detected magnetic resonance for fluorescent single nanodiamond in cell and c.elegance(26. Measurements,Poster)

Yoshinari Yohsuke, Kumiya Yuta, Sugi Takuma, Igarashi Ryuji, Sotoma Shingo, Shirakawa Masahiro, Harada Yoshie

Seibutsu Butsuri　Vol. 53　No. 1　2013

Characterization of SRA-Methylated DNA Complexes Dynamics Related to Chromatin Structure Regulation

Yong-Woon Han, Hiroaki Yokota, Mariko Ariyoshi, Yasuo Tsunaka, Takuma Iwasa, Ryuji Yokokawa, Ryo Hiramatsu, Daichi Chiba, Teruo Ono, Yoshie Harada

BIOPHYSICAL JOURNAL　Vol. 104　No. 2　p. 255A-255A　2013/01　Research paper (scientific journal)

疾患克服を目指したケミカルバイオロジー 生きた細胞内の温度イメージング

岡部 弘基, 内山 聖一, 稲田 のりこ, 原田 慶恵, 船津 高志

日本生化学会大会プログラム・講演要旨集　Vol. 85回　p. 1S16-2　2012/12

Binding of hairpin pyrrole and imidazole polyamides to DNA: relationship between torsion angle and association rate constants

Yong-Woon Han, Tomoko Matsumoto, Hiroaki Yokota, Gengo Kashiwazaki, Hironobu Morinaga, Kaori Hashiya, Toshikazu Bando, Yoshie Harada, Hiroshi Sugiyama

NUCLEIC ACIDS RESEARCH　Vol. 40　No. 22　p. 11510-11517　2012/12　Research paper (scientific journal)

「小さくてもキラリと光る仕事を」再考

原田 慶恵

生物物理　Vol. 52　No. 6　p. 263-263　2012/11/25

Real-Time Background-Free Selective Imaging of Fluorescent Nanodiamonds in Vivo

Ryuji Igarashi, Yohsuke Yoshinari, Hiroaki Yokota, Takuma Sugi, Fuminori Sugihara, Kazuhiro Ikeda, Hitoshi Sumiya, Shigenori Tsuji, Ikue Mori, Hidehito Tochio, Yoshie Harada, Masahiro Shirakawa

NANO LETTERS　Vol. 12　No. 11　p. 5726-5732　2012/11　Research paper (scientific journal)

蛍光性温度センサーを利用した細胞内の温度分布計測

岡部 弘基, 稲田 のりこ, 郷田 千恵, 原田 慶恵, 船津 高志, 内山 聖一

日本分析化学会講演要旨集　Vol. 61年会　p. 136-136　2012/09

Direct Observation of Strand Passage by DNA-Topoisomerase and Its Limited Processivity

Katsunori Yogo, Taisaku Ogawa, Masahito Hayashi, Yoshie Harada, Takayuki Nishizaka, Kazuhiko Kinosita

PLOS ONE　Vol. 7　No. 4　2012/04　Research paper (scientific journal)

Round-Table Discussion on "Future of Biophysics"

Ishimori Koichiro, Ito Etsuro, Takada Shoji, Terakita Akihisa, Harada Yoshie, Mochizuki Atsushi, Yasuda Kenji, Wakatsuki Soichi, Kandori Hideki, Kidera Akinori

Seibutsu Butsuri　Vol. 52　No. 2　p. 83-95　2012/03/25

Intracellular temperature mapping with a fluorescent polymeric thermometer and fluorescence lifetime imaging microscopy

Kohki Okabe, Noriko Inada, Chie Gota, Yoshie Harada, Takashi Funatsu, Seiichi Uchiyama

NATURE COMMUNICATIONS　Vol. 3　2012/02　Research paper (scientific journal)

1PS042 Multiple kinesin molecules coordinate to ensure the long-distance walking : a DNA-kinesin hybrid nanomachine study(The 50th Annual Meeting of the Biophysical Society of Japan)

Miyazono Yuya, Endo Masayuki, Ueda Takuya, Sugiyama Hiroshi, Harada Yoshie, Tadakuma Hisashi

Seibutsu Butsuri　Vol. 52　2012

1SD-01 Characterization of Protein-DNA complexes dynamics related to Chromatin structure regulation using Single-Molecule Techniques(1SD Molecular Dynamics in the Nucleus and Gene Regulation,Symposium,The 50th Annual Meeting of the Biophysical Society of Japan)

Han Yong-Woon, Harada Yoshie

Seibutsu Butsuri　Vol. 52　2012

3PS031 Live-cell secretion imaging assay of the inflammatory cytokine from human monocytes(The 50th Annual Meeting of the Biophysical Society of Japan)

Shirasaki Yoshitaka, Shimura Nanako, Suzuki Nobutake, Izawa Kazushi, Nakahara Asahi, Yamagishi Mai, Harada Yoshie, Shouji Shuichi, Nishikomori Ryuta, Ohara Osamu

Seibutsu Butsuri　Vol. 52　2012

1YS0915 Imaging of temperature distribution in a living cell(The 50th Annual Meeting of the Biophysical Society of Japan)

Okabe Kohki, Uchiyama Seiichi, Inada Noriko, Harada Yoshie, Funatsu Takashi

Seibutsu Butsuri　Vol. 52　2012

3PT111 Construction and functional analysis of DNA origami base DNA-RNAP hybrid nanomachine(The 50th Annual Meeting of the Biophysical Society of Japan)

Masubuchi Takeya, Tadakuma Hisashi, Endo Masayuki, Sugiyama Hiroshi, Harada Yoshie, Ueda Takuya

Seibutsu Butsuri　Vol. 52　2012

3C1046 Single-molecule visualization of a non-hexameric helicase reveals active roles of its oligomeric forms in DNA unwinding(Nucleic Acids,Oral Presentation,The 50th Annual Meeting of the Biophysical Society of Japan)

Yokota Hiroaki, Harada Yoshie

Seibutsu Butsuri　Vol. 52　2012

Direct Visualization of the Movement of a Single T7 RNA Polymerase and Transcription on a DNA Nanostructure

Masayuki Endo, Koichi Tatsumi, Kosuke Terushima, Yousuke Katsuda, Kumi Hidaka, Yoshie Harada, Hiroshi Sugiyama

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION　Vol. 51　No. 35　p. 8778-8782　2012　Research paper (scientific journal)

線形アンチセンスプローブを用いた生細胞内のmRNAイメージング

岡部 弘基, 張 ジュンウェイ, 原田 慶恵, 船津 高志

バイオイメージング　Vol. 20　No. 2　p. 143-144　2011/08

Facilitated Intracellular Transport of TrkA by an Interaction with Nerve Growth Factor

Mami Nomura, Takeharu Nagai, Yoshie Harada, Tomomi Tani

DEVELOPMENTAL NEUROBIOLOGY　Vol. 71　No. 7　p. 634-649　2011/07　Research paper (scientific journal)

Real time monitoring of endogenous cytoplasmic mRNA using linear antisense 2′-O-methyl RNA probes in living cells

Kohki Okabe, Yoshie Harada, Junwei Zhang, Hisashi Tadakuma, Tokio Tani, Takashi Funatsu

Nucleic Acids Research　Vol. 39　No. 4　p. e20-e20　2011/03　Research paper (scientific journal)

1M1548 P46 Single-molecule visualization of a AAA^+ DNA recombination ATPase with zero-mode waveguides toward elucidation of its hexamer formation(Molecular motor 2,The 49th Annual Meeting of the Biophysical Society of Japan)

Iwasa Takuma, Han Yongwoon, Yokota Hiroaki, Yokokawa Ryuji, harada Yoshie

Seibutsu Butsuri　Vol. 51　2011

1M1524 Constructing DNA-kinesin hybrid-nanomachine using the DNA-tile scaffold(Molecular motor 2,The 49th Annual Meeting of the Biophysical Society of Japan)

Miyazono Yuya, Endo Masayuki, Ueda Takuya, Sugiyama Hiroshi, Harada Yoshie, Tadakuma Hisashi

Seibutsu Butsuri　Vol. 51　p. S64-S65　2011

1C1548 Direct observation of P1 plasmid movement on an optical microscope(Nucleic acid,The 49th Annual Meeting of the Biophysical Society of Japan)

Han Yong-Woon, Hwang Ling-Chin, Vecchiarelli Anthony G., Mizuuchi Michiyo, Funnell Barbara E., Harada Yoshie, Mizuuchi Kiyoshi

Seibutsu Butsuri　Vol. 51　p. S35-S36　2011

生体分子・細胞を「見る」「測る」「解る」ための新規分析法 生きた単一細胞内の内在性mRNAのイメージングと定量

岡部 弘基, 原田 慶恵, 船津 高志

日本薬学会年会要旨集　Vol. 130年会　No. 1　p. 314-314　2010/03

Early Research in Biophysics Award : Report on the Fifth Award Selection Process

HARADA Yoshie

Seibutsu Butsuri　Vol. 50　No. 1　p. 42-43　2010/01/25

Strain through the neck linker ensures processive runs: a DNA-kinesin hybrid nanomachine study

Yuya Miyazono, Masahito Hayashi, Peter Karagiannis, Yoshie Harada, Hisashi Tadakuma

The EMBO Journal　Vol. 29　No. 1　p. 93-106　2010/01/06　Research paper (scientific journal)

2P332 Quantitative measurement of protein secretion from a single cell in real time(The 48th Annual Meeting of the Biophysical Society of Japan)

Shirasaki Yoshitaka, Yamagishi Mai, Nakamura Asahi, Sasuga Yasuhiro, Suzuki Nobutake, Shimura Nanako, Kawai Kentaro, Mizuno Jun, Shoji Shuichi, Harada Yoshie, Ohara Osamu

Seibutsu Butsuri　Vol. 50　No. 2　2010

2P177 Strain through the neck linker ensures processive runs : a DNA-kinesin hybrid nanomachine study(The 48th Annual Meeting of the Biophysical Society of Japan)

Miyazono Yuya, Hayashi Masahito, Karagiannis Peter, Harada Yoshie, Tadakuma Hisashi

Seibutsu Butsuri　Vol. 50　No. 2　2010

1P125 Fixed interaction between the Escherichia coli RuvA and RuvB proteins during branch migration of Holliday junction(Nucleic acid binding proteins,The 48th Annual Meeting of the Biophysical Society of Japan)

Han YongWoon, Yokota Hiroaki, Hishida Takashi, Iwasaki Hiroshi, Hayashi Masahito, Shinagawa Hideo, Harada Yoshie

Seibutsu Butsuri　Vol. 50　No. 2　2010

Single-molecule Visualization of Binding Modes of Helicase to DNA on PEGylated Surfaces

Hiroaki Yokota, Yong-Woon Han, Jean-Francois Allemand, Xu Guang Xi, David Bensimon, Vincent Croquette, Yoshihiro Ito, Yoshie Harada

CHEMISTRY LETTERS　Vol. 38　No. 4　p. 308-309　2009/04　Research paper (scientific journal)

Hydrophilic Fluorescent Nanogel Thermometer for Intracellular Thermometry

Chie Gota, Kohki Okabe, Takashi Funatsu, Yoshie Harada, Seiichi Uchiyama

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY　Vol. 131　No. 8　p. 2766-+　2009/03　Research paper (scientific journal)

1P-269 Real time monitoring of endogenous mRNA in living cells using linear antisense probe(Bioimaging, The 47th Annual Meeting of the Biophysical Society of Japan)

Okabe Kohki, Harada Yoshie, Funatsu Takashi

Seibutsu Butsuri　Vol. 49　p. S104-S105　2009

3P-253 Single-molecule observation or DNA-hehcase interaction(Bioimaging,Oral Presentations,The 47th Annual Meeting of the Biophysical Society of Japan)

Yokota Hiroaki, Chujo Yuko, Nishimoto Emi, Harada Yoshie

Seibutsu Butsuri　Vol. 49　2009

Single-Cell Chemical Lysis Method for Analyses of Intracellular Molecules Using an Array of Picoliter-Scale Microwells

Yasuhiro Sasuga, Tomoyuki Iwasawa, Kayoko Terada, Yoshihiro Oe, Hiroyuki Sorimachi, Osamu Ohara, Yoshie Harada

ANALYTICAL CHEMISTRY　Vol. 80　No. 23　p. 9141-9149　2008/12　Research paper (scientific journal)

細胞機能の3次元ナノイメージング 生きた細胞内における内在性mRNAの検出と定量(Three dimensional nano-imaging of cellular functions Real time monitoring and quantitation of endogenous mRNA in single living cells)

岡部 弘基, 原田 慶恵, 船津 高志

日本細胞生物学会大会講演要旨集　Vol. 60回　p. 78-78　2008/06

生きた細胞内における内在性mRNAの検出と定量(Real time monitoring and quantitation of endogenous mRNA in single living cells)

岡部 弘基, 原田 慶恵, 船津 高志

日本細胞生物学会大会講演要旨集　Vol. 60回　p. 117-117　2008/06

1分子が見えた！

原田慶恵

細胞工学　Vol. 27　No. 5　p. 425-425　2008/05

2P-332 Determination of the number of DNA helicase UvrD in its functional unit by single-molecule analysis(The 46th Annual Meeting of the Biophysical Society of Japan)

Chujo Yuko, Harada Yoshie, Yokota Hiroaki

Seibutsu Butsuri　Vol. 48　2008

2P-331 Single-molecule observation of DNA-hehcase interaction(The 46th Annual Meeting of the Biophysical Society of Japan)

Yokota Hiroaki, Han Yong-Woon, Allemand Jean-Francois, Xi Xuguang, Croquette Vincent, Bensimon David, Harada Yoshie

Seibutsu Butsuri　Vol. 48　2008

3P-054 A novel protein array using microbeads aligned in a microfluidic chip(The 46th Annual Meeting of the Biophysical Society of Japan)

Ooe Yoshihiro, Sasuga Yasuhiro, Ohara Osamu, Harada Yoshie

Seibutsu Butsuri　Vol. 48　p. S135-S136　2008

1P-158 DNA-kinesin Hybrid-nanomachine reveals that optimized layout is crucical for kinesin's processive run(The 46th Annual Meeting of the Biophysical Society of Japan)

Miyazono Yuya, Hayashi Masahito, Harada Yoshie, Tadakuma Hisashi

Seibutsu Butsuri　Vol. 48　2008

3P-326 Quantification of mRNA and its corresponding protein at the same time in single living mammalian cells(The 46th Annual Meeting of the Biophysical Society of Japan)

Kobayashi Toru, Okabe Kohki, Harada Yoshie, Funatsu Takashi

Seibutsu Butsuri　Vol. 48　2008

生きた単一細胞における内在性mRNAのリアルタイム定量(Real time quantitation of the endogenous mRNA in single living cells)

岡部 弘基, 船津 高志, 原田 慶恵

生物物理　Vol. 47　No. Suppl.1　p. S282-S282　2007/11

細胞質の分子篩効果によるmRNAの細胞質内局在化(Subcellular localization of mRNA is attributed to molecular sieving effect)

山岸 舞, 石浜 陽, 白崎 善隆, 貴家 康尋, 寺田 佳代子, 原田 慶恵, 船津 高志

生物物理　Vol. 47　No. Suppl.1　p. S282-S282　2007/11

Direct observation of the reversible unwinding of a single DNA molecule caused by the intercalation of ethidium bromide

Masahito Hayashi, Yoshie Harada

NUCLEIC ACIDS RESEARCH　Vol. 35　No. 19　2007/10　Research paper (scientific journal)

世界物理年に学んだこと

原田慶恵

教師の広場　No. 152　p. 4-5　2007/06

一分子蛍光法

原田 慶恵

化学と生物　Vol. 45　No. 5　p. 340-346　2007/05/01

Inhibition of reversed electron transfer and proton transport in the beef heart cytochrome bc(1) complex by chemical modification

Toshiaki Miki, Masato Umeda, Yoshie Harada

JOURNAL OF BIOCHEMISTRY　Vol. 141　No. 3　p. 377-387　2007/03　Research paper (scientific journal)

Visualization of RecA filaments and DNA by fluorescence microscopy

Taro Nishinaka, Yuko Doi, Makiko Hashimoto, Reiko Hara, Takehiko Shibata, Yoshie Harada, Kazuhiko Kinosita, Hiroyuki Noji, Eiji Yashima

JOURNAL OF BIOCHEMISTRY　Vol. 141　No. 2　p. 147-156　2007/02　Research paper (scientific journal)

3P154 Observation of transcription bubble formation during transcription initiation(Molecular motors,Poster Presentations)

Kato Yuko, Hayashi Masahito, Harada Yoshie

Seibutsu Butsuri　Vol. 47　2007

3P193 The effect of nerve growth factor on the regenerating process of severed axons(Cell biological problems-adhesion, motility, cytoskeleton, signaling, and membrane,Poster Presentations)

Nomura Mami, Harada Yoshie, Tani Tomomi

Seibutsu Butsuri　Vol. 47　2007

3P320 Single-molecule observation of DNA/helicase interaction by novel microscopy(Bioimaging. Behavior. Development and differentiation. Molecular genetics. Others,Oral Presentations)

Yokota Hiroaki, Han Yong-Woon, Allemand Jean-Francois, Xuguang Xi, Vincent Croquette, Bensimon David, Harada Yoshie

Seibutsu Butsuri　Vol. 47　2007

3P317 Real time quantitation of the endogenous mRNA in single living cells(Bioimaging. The genesis of life, and biological evolution,Oral Presentations)

Okabe Kohki, Funatsu Takashi, Harada Yoshie

Seibutsu Butsuri　Vol. 47　2007

3P319 Single-molecule observation of DNA binding/unwinding by DNA helicase UvrD(Bioimaging. Behavior. Development and differentiation. Molecular genetics. Others,Oral Presentations)

Chujo Yuko, Yokota Hiroaki, Harada Yoshie

Seibutsu Butsuri　Vol. 47　2007

3P318 Subcellular localization of mRNA is attributed to molecular sieving effect(Bioimaging. Behavior. Development and differentiation. Molecular genetics. Others,Oral Presentations)

Yamagishi Mai, Ishihama Yo, Shirasaki Yoshitaka, Sasuga Yasuhiro, Terada Kayoko, Harada Yoshie, Funatsu Takashi

Seibutsu Butsuri　Vol. 47　2007

バイオマシン・生物分子モーター

原田慶恵, 横田浩章

細胞工学　Vol. 25　No. 8　p. 878-883　2006/08

Direct observation of DNA rotation during branch migration of Holliday junction DNA by Escherichia coli RuvA-RuvB protein complex

Yong-Woon Han, Tomomi Tani, Masahito Hayashi, Takashi Hishida, Hiroshi Iwasaki, Hideo Shinagawa, Yoshie Harada

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　Vol. 103　No. 31　p. 11544-11548　2006/08　Research paper (scientific journal)

2P525 Development of a microscopic platform for single-cell analyses using picoliter microwell array(52. Bio-imaging,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)

Sasuga Yasuhiro, Iwasawa Tomoyuki, Terada Kayoko, Sorimachi Hiroyuki, Ohara Osamu, Harada Yoshie

Seibutsu Butsuri　Vol. 46　No. 2　2006

2P513 Real Time Imaging of Specific Messenger RNA in a Living Cell Using Artificial Nucleic Acids(52. Bio-imaging,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)

Okabe Kohki, Harada Yoshie, Funatsu Takashi

Seibutsu Butsuri　Vol. 46　No. 2　2006

1P556 Novel microscopy for simultaneous single molecule measurement of DNA/protein interaction(26. Single molecule biophysics,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)

Yokota Hiroaki, Han Yong-Woon, Allemand Jean-Francois, Xi Xugang, Croquette Vincent, Bensimon David, Harada Yoshie

Seibutsu Butsuri　Vol. 46　No. 2　2006

1P174 Labeling of iron-sulfur protein with a fluorescent dye to detect its conformation change in the cytochrome bc_1 complex(5. Heme protein,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)

Miki Toshiaki, Sakamoto Shun, Yokota Hiroaki, Harada Yoshie

Seibutsu Butsuri　Vol. 46　No. 2　2006

1P297 Direct observation of T7 RNA polymerase rotation during transcription.(9. Molecular motor (I),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)

Kato Yuko, Yokota Hiroaki, Hayashi Masahito, Terada Kayoko, Sasuga Yasuhiro, Harada Yoshie

Seibutsu Butsuri　Vol. 46　No. 2　2006

Development of a microscopic platform for real-time monitoring of biomolecular interactions

Y Sasuga, T Tani, M Hayashi, H Yamakawa, O Ohara, Y Harada

GENOME RESEARCH　Vol. 16　No. 1　p. 132-139　2006/01　Research paper (scientific journal)

Single Molecule Analyses on the Behavior of Nerve Growth Factor during the Activation of Axonal Outgrowth

TANI Tomomi, HARADA Yoshie

Biophysics　Vol. 45　No. 6　p. 320-323　2005/11/25

１個の生体分子の運動を観る

原田慶恵

総研大ジャーナル　2005/10

ビーズを用いた生体分子間相互作用解析技術の開発

原田 慶恵

BIO INDUSTRYシーエムシー出版　Vol. 22　No. 10　p. 66-74　2005/10

Structure-function analysis of the three domains of RuvB DNA motor protein

T Ohnishi, T Hishida, Y Harada, H Iwasaki, H Shinagawa

JOURNAL OF BIOLOGICAL CHEMISTRY　Vol. 280　No. 34　p. 30504-30510　2005/08　Research paper (scientific journal)

分子イメージングによる分子間相互作用解析法の開発

貴家康尋, 原田慶恵

バイオテクノロジージャーナル　Vol. 5　No. 3　p. 303-305　2005/05

Trafficking of a ligand-receptor complex on the growth cones as an essential step for the uptake of nerve growth factor at the distal end of the axon: A single-molecule analysis

T Tani, Y Miyamoto, KE Fujimori, T Taguchi, T Yanagida, Y Sako, Y Harada

JOURNAL OF NEUROSCIENCE　Vol. 25　No. 9　p. 2181-2191　2005/03　Research paper (scientific journal)

3SF55 Single molecule approach to the function of biomolecules

Harada Y.

Seibutsu Butsuri　Vol. 45　2005

2P136 Single molecular measurement of the binding constant of ethidium bromide to DNA

Hayashi M., Harada Y.

Seibutsu Butsuri　Vol. 45　2005

3P094 Attempt of real-time visualization of the conformational change in mitochondrial cytochrome bc_1 complex

Miki T., Tani T., Harada Y.

Seibutsu Butsuri　Vol. 45　2005

1P120 Nucleotide-dependent Morphological Analysis of the RecA-DNA Filamentous Complex

Nishinaka T., Doi Y., Hashimoto M., Shibata T., Harada Y., Kinosita K., Noji H., Yashima E.

Seibutsu Butsuri　Vol. 45　2005

1P128 Kinetic analysis of Holliday junction branch migration mediated by RuvA-RuvB

Han Y.-W., Tani T., Hayashi M., Yokota H., Hishida T., Iwasaki H., Shinagawa H., Harada Y.

Seibutsu Butsuri　Vol. 45　2005

1P349 Real time imaging of specific messenger RNA in a living cell using artifical antisense nucleic acids

Okabe K., Ikeda H., Harada Y., Funatsu T.

Seibutsu Butsuri　Vol. 45　2005

1P333 Protein expression profiling for single-cell using microbead array technology

Sasuga Y., Terada K., Tani T., Ohara O., Harada Y.

Seibutsu Butsuri　Vol. 45　2005

1P327 Development of a novel mechano-fluorescence microscope for measurement of DNA/protein interaction at single molecule level II

Yokota H., Han YW., Allemand Jean-Francois, Xi Xueuang, Croquette Vincent, Bensimon David, Harada Y.

Seibutsu Butsuri　Vol. 45　2005

1P348 Labeling and imaging of mRNAs in living cells

Yamagishi M., Sasuga Y., Terada K., Harada Y., Funatsu T.

Seibutsu Butsuri　Vol. 45　2005

蛍光１分子イメージング.

谷知己, 原田慶恵

蛋白質 核酸 酵素　Vol. 49　No. 11　p. 1647-1651　2004/11

Studies on Biomolecules Using Single Molecule Imaging and Manipulation Techniques.

Yoshie Harada

Sci. Tech. Adv. Mat.　Vol. 5　p. 709-713　2004/08　Research paper (scientific journal)

2P097 Direct observation of the RuvA-RuvB mediated branch migration of Holliday junction DNA with single-molecule analysis

Han Y.-W., Tani T., Hayashi M., Hishida T., Iwasaki H., Shinagawa H., Harada Y.

Seibutsu Butsuri　Vol. 44　2004

2P088 Reconstitution of mitochondrial cytochrome bc_1 complex with recombinant iron-sulfur protein

Miki T., Sasuga Y., Harada Y.

Seibutsu Butsuri　Vol. 44　2004

2P277 Direct measurement of the change of the double helical structure in a single DNA molecule

Hayashi M., Harada Y.

Seibutsu Butsuri　Vol. 44　2004

2P278 Development of the molecular interaction analysis system using microbeads array

Sasuga Y., Ohara O., Tani T., Harada Y.

Seibutsu Butsuri　Vol. 44　2004

2P158 Motile responses of dorsal root ganglion growth cones upon application of nerve growth factor

Tani T., Harada Y.

Seibutsu Butsuri　Vol. 44　2004

2P178 Association-dissociation kinetics of beta-actin mRNA binding to ZBP1

Egawa T., Kurama H., Sasuga Y., Harada Y., Funatsu T.

Seibutsu Butsuri　Vol. 44　2004

生体分子機能を観察できる光学顕微鏡システムの開発

貴家康尋, 原田 慶恵

月刊薬事　Vol. 46　No. 5　p. 149-154　2004

熱ゆらぎを利用して動く？-モータータンパク質の新しい運動モデル

原田慶恵

Vol. 58　No. 11　p. 48-49　2003/11

RNAポリメラーゼによるDNAの回転をみる

原田慶恵

実験医学　Vol. 21　No. 13　p. 1800-1801　2003/09

生理学実験法講座「1分子生理学」 DNAモーターの1分子機能解析

原田慶恵

日生誌　Vol. 65　No. 7,8　p. 225-231　2003/07

One-directional transport and accumulation of NGF on the growth cones : A single molecule study.

Tani T., Harada Y.

Seibutsu Butsuri　Vol. 43　2003

「生物物理」分野の研究

原田慶恵

共同参画２１　2003/01

1分子の動きを見て生体分子の機能を探る

原田慶恵

光技術コンタクト　Vol. 40　No. 3　p. 19-26　2002/12

3N1545 Imaging of transport and localization of beta-actin mRNA using fluorescence microscopy

Kurama H., Sasuga Y., Tadakuma H., Egawa T., Funatsu T., Harada Y.

Seibutsu Butsuri　Vol. 42　No. 2　2002

RNAポリメラーゼはDNAを回転させて転写する

原田慶恵

化学と生物　Vol. 39　No. 7　p. 420-421　2001/07

RNAポリメラーゼによるDNAの回転の可視化

原田慶恵

細胞工学　Vol. 20　No. 5　p. 636-637　2001/05

Direct observation of DNA rotation during transcription by RNA polymerase

Harada Y.

Seibutsu Butsuri　Vol. 41　2001

Direct observation of DNA rotation during transcription by Escherichia coli RNA polymerase

Y Harada, O Ohara, A Takatsuki, H Itoh, N Shimamoto, K Kinosita

NATURE　Vol. 409　No. 6816　p. 113-115　2001/01　Research paper (scientific journal)

Stepping rotation of F-1-ATPase visualized through angle-resolved single-fluorophore imaging

K Adachi, R Yasuda, H Noji, H Itoh, Y Harada, M Yoshida, K Kinosita

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　Vol. 97　No. 13　p. 7243-7247　2000/06　Research paper (scientific journal)

Video imaging capable of measuring short lifetime of fluorescent nucleotide bound to ATPase site

Chaen S., Shirakawa I., Harada Y.

Seibutsu Butsuri　Vol. 40　2000

DNA分子に結び目をつくる

原田慶恵, 木下一彦

遺伝　Vol. 53　No. 10　p. 10-12　1999/10

分子のひもを結ぶ

原田 慶恵

三田評論　Vol. 1015　p. 68-69　1999/07/01

Tying a molecular knot with optical tweezers

Y Arai, R Yasuda, K Akashi, Y Harada, H Miyata, K Kinosita, H Itoh

NATURE　Vol. 399　No. 6735　p. 446-448　1999/06　Research paper (scientific journal)

Single-molecule imaging of RNA polymerase-DNA interactions in real time

Y Harada, T Funatsu, K Murakami, Y Nonoyama, A Ishihama, T Yanagida

BIOPHYSICAL JOURNAL　Vol. 76　No. 2　p. 709-715　1999/02　Research paper (scientific journal)

Single Molecule Imaging of DNA-RNA Polymerase Interaction by Evanscent Field(Special Issue Imaging and Manipulation of DNA)

HARADA Yoshie

Journal of the Japan Society of Precision Engineering　Vol. 65　No. 2　p. 205-208　1999/02

Measurement of the torsional rigidity of single DNA molecules

Hada Y., Harada Y., Kinoshita K.Jr.

Seibutsu Butsuri　Vol. 39　1999

Manipulation with magnet tweezers

Takenaka K., Harada Y., Itoh H., Kinoshita K.Jr.

Seibutsu Butsuri　Vol. 39　1999

蛍光顕微鏡システムによる生体分子の1分子イメージング

原田慶恵, 船津高志

細胞工学　Vol. 17　No. 4　p. 626-633　1998/04

Single molecule imaging and nanomanipulation of biomolecules

Y Harada, T Funatsu, M Tokunaga, K Saito, H Higuchi, Y Ishii, T Yanagida

METHODS IN CELL BIOLOGY, VOL 55　Vol. 55　p. 117-128　1998

Imaging and nano-manipulation of single biomolecules

Takashi Funatsu, Yoshie Harada, Hideo Higuchi, Makio Tokunaga, Kiwamu Saito, Yoshiharu Ishii, Ronald D. Vale, Toshio Yanagida

Biophysical Chemistry　Vol. 68　No. 1-3　p. 63-72　1997/10　Research paper (scientific journal)

生体分子における１分子イメージング・操作

原田慶恵

オプトロニクス　Vol. 188　No. 8　p. 140-144　1997/08

タンパク質分子のイメージング

原田慶恵

応用物理　Vol. 66　No. 5　p. 490-491　1997/05

Single molecular assay of individual ATP turnover by a myosin-GFP fusion protein expressed is vitro

Atsuko Hikikoshi Iwane, Takashi Funatsu, Yoshie Harada, Makio Tokunaga, Osamu Ohara, Sayuri Morimoto, Toshio Yanagida

FEBS Letters　Vol. 407　No. 2　p. 235-238　1997/04/28　Research paper (scientific journal)

Coupling of protein surface hydrophobicity change to ATP hydrolysis by myosin motor domain

M Suzuki, J Shigematsu, Y Fukunishi, Y Harada, T Yanagida, T Kodama

BIOPHYSICAL JOURNAL　Vol. 72　No. 1　p. 18-23　1997/01　Research paper (scientific journal)

Direct inhibition of the actomyosin motility by local anesthetics in vitro

Y Tsuda, T Mashimo, Yoshiya, I, K Kaseda, Y Harada, T Yanagida

BIOPHYSICAL JOURNAL　Vol. 71　No. 5　p. 2733-2741　1996/11　Research paper (scientific journal)

分子モーター１分子の動きを観る

船津高志, 原田慶恵

実験医学　Vol. 14　No. 16　p. 2255-2257　1996/10

Direct observation of single kinesin molecules moving along microtubules

RD Vale, T Funatsu, DW Pierce, L Romberg, Y Harada, T Yanagida

NATURE　Vol. 380　No. 6573　p. 451-453　1996/04　Research paper (scientific journal)

ゾウリムシにおける食胞形成

三島祥二, 原田慶恵, 菊池嘉子

茨城大学教養部紀要　1996/01　Research paper (scientific journal)

Multiple- and single-molecule analysis of the actomyosin motor by nanometer piconewton manipulation with a microneedle: Unitary steps and forces

A Ishijima, H Kojima, H Higuchi, Y Harada, T Funatsu, T Yanagida

BIOPHYSICAL JOURNAL　Vol. 70　No. 1　p. 383-400　1996/01　Research paper (scientific journal)

アクトミオシン滑り運動実験法のポイント

原田慶恵

生物物理　Vol. 35　No. 6　p. 51-52　1995/12

１分子の酵素反応を見る

原田慶恵

パリティ　Vol. 10　No. 11　p. 28-30　1995/11

局所麻酔薬とタンパク質の分子機能

津田祐里, 真下節, 吉矢生人, 原田慶恵, 柳田敏雄

麻酔と蘇生　Vol. 31　No. 2　p. 97-101　1995/06

Biological Molecular Motor

Vol. 34　No. 4　p. 335-335　1995/04

Effects of local anesthetics on the ATPase activity and motility of actomyosin directly studied by in vitro motility assay

Y TSUDA, T MASHIMO, YOSHIYA, I, Y HARADA, T YANAGIDA

PROGRESS IN ANESTHETIC MECHANISM, VOL 3, SPECIAL ISSUE, 1995　p. 290-295　1995　Research paper (international conference proceedings)

Imaging of single fluorescent molecules and individual ATP turnovers by single myosin molecules in aqueous solution

Takashi Funatsu, Yoshie Harada, Makio Tokunaga, Kiwamu Saito, Toshio Yanagida

Nature　Vol. 374　No. 6522　p. 555-559　1995　Research paper (scientific journal)

生物分子モーターのナノ操作と動作原理

原田慶恵, 齋籐究, 石島秋彦, 柳田敏雄

光学　Vol. 23　No. 5　p. 290-296　1994/05

SINGLE-MOLECULE ANALYSIS OF THE ACTOMYOSIN MOTOR USING NANO-MANIPULATION

A ISHIJIMA, Y HARADA, H KOJIMA, T FUNATSU, H HIGUCHI, T YANAGIDA

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　Vol. 199　No. 2　p. 1057-1063　1994/03　Research paper (scientific journal)

Force-generating domain of myosin motor

Shinji Itakura, Hisashi Yamakawa, Yoko Yano Toyoshima, Akihiko Ishijima, Takaaki Kojima, Yoshie Harada, Toshio Yanagida, Takeyuki Wakabayashi, Kazuo Sutoh

Biochemical and Biophysical Research Communications　Vol. 196　No. 3　p. 1504-1510　1993/11/15　Research paper (scientific journal)

NANO-MANIPULATION OF ACTOMYOSIN MOLECULAR MOTORS IN-VITRO - A NEW WORKING PRINCIPLE

T YANAGIDA, Y HARADA, A ISHIJIMA

TRENDS IN BIOCHEMICAL SCIENCES　Vol. 18　No. 9　p. 319-324　1993/09　Research paper (scientific journal)

Do the two heads of the myosin molecule function independently or cooperatively？

Yoshie Harada, Toshio Yanagida

Molecular and Cellular Biology　p. 464-465　1993/01

COUPLING BETWEEN ATPASE AND FORCE-GENERATING ATTACHMENT-DETACHMENT CYCLES OF ACTOMYOSIN IN-VITRO

T YANAGIDA, A ISHIJIMA, K SAITO, Y HARADA

MECHANISM OF MYOFILAMENT SLIDING IN MUSCLE CONTRACTION　Vol. 332　p. 339-347　1993　Research paper (international conference proceedings)

メカノカップリングの本質に迫る

柳田敏雄, 原田慶恵

日本の科学と技術　Vol. 32　No. 262　p. 41-47　1991/07

Chemomechanical coupling in actomyosin system: An approach by in vitro movement assay and kinetic analysis of ATP hydrolysis by shortening myofibrils

Toshio Yanagida, Yoshie Harada, Takao Kodama

Advances in Biophysics　Vol. 27　No. C　p. 237-257　1991　Research paper (scientific journal)

0.1nm 分解能で生物分子エンジン1つの動きを計測する

石島秋彦, 原田慶恵, 柳田敏雄

計測と制御　Vol. 29　No. 12　p. 1123-1130　1990/12

MECHANOCHEMICAL COUPLING IN ACTOMYOSIN ENERGY TRANSDUCTION STUDIED BY INVITRO MOVEMENT ASSAY

Y HARADA, K SAKURADA, T AOKI, DD THOMAS, T YANAGIDA

JOURNAL OF MOLECULAR BIOLOGY　Vol. 216　No. 1　p. 49-68　1990/11　Research paper (scientific journal)

蛍光ラベル

原田慶恵

実験医学　Vol. 8　No. 5　p. 129-133　1990/05

運動蛋白質の分子直視・分子超微操作

柳田敏雄, 原田慶恵

蛋白質 核酸 酵素　Vol. 34　No. 13　p. 1784-1792　1989/12

The elementary process in the actomyosin energy transduction system.

Yoshie Harada, Toshio Yanagida

Muscle Energetics　p. 27-36　1989/06

Caged ATP

原田慶恵

生体の科学　Vol. 40　No. 4　p. 394-395　1989/04

アクトミオシン系のエネルギー変換機構

原田慶恵

生物物理　Vol. 28　No. 5　p. 219-223　1988/10

Direct Observation of Molecular Motility by Light Microscopy

HARADA Y.

Cell Motility and the Cytoskeleton　Vol. 10　p. 71-76　1988/05　Research paper (scientific journal)

Minimum structural unit required for energy transduction in muscle.

Toshio Yanagida, Yoshie Harada

Molecular Mechanism of Muscle Contraction　p. 277-287　1988/03　Research paper (scientific journal)

筋肉におけるエネルギー変換の素過程

原田慶恵, 柳田敏雄

生体の科学　Vol. 39　No. 2　p. 110-114　1988/02

Approach to the Riddle of the Mechanism of Muscle Contraction

Harada Yosie, Prochniewicz-Nakayama Ewa, Yanagida Toshio

Vol. 42　No. 5　p. 424-432　1987/05

Sliding movement of single actin filaments on one-headed myosin filaments

Yoshie Harada, Akira Noguchi, Akiyoshi Kishino, Toshio Yanagida

Nature　Vol. 326　No. 6115　p. 805-808　1987　Research paper (scientific journal)

アクチンとATP

原田慶恵, 柳田敏雄

遺伝　Vol. 40　No. 7　p. 37-42　1986/07

Paramecium multimicronucleatum の交配活性を発現しないクローン

三島祥二, 原田慶恵

茨城大学教養部紀要　No. 17　p. p81-85　1985/01　Research paper (scientific journal)