Engineered coagulation factor VIII with enhanced secretion and coagulation potential for hemophilia A gene therapy

Yuji Kashiwakura, Yuto Nakajima, Kio Horinaka, Tiago Jose da Silva Lopes, Yuma Furuta, Yuki Yamaguchi, Nemekhbayar Baatartsogt, Morisada Hayakawa, Yuko Katakai, Susumu Uchiyama, Osamu Nureki, Keiji Nogami, Tsukasa Ohmori

2024/12/09

Physicochemical and biological impacts of light stress on adeno-associated virus serotype 6

Rie Takino, Yuki Yamaguchi, Takahiro Maruno, Ekaputra Ramadhani, Misaki Furukawa, Tetsuo Torisu, Susumu Uchiyama

Molecular Therapy - Methods & Clinical Development　2024/12　Research paper (scientific journal)

Multimass Analysis of Adeno-Associated Virus Vectors by Orbitrap-Based Charge Detection Mass Spectrometry

Ryoji Nakatsuka, Yuki Yamaguchi, Kiichi Hirohata, Saki Shimojo, Makoto Murakami, Mark Allen Vergara Rocafort, Yasuo Tsunaka, Mitsuko Fukuhara, Tetsuo Torisu, Susumu Uchiyama

Analytical Chemistry　2024/10/22　Research paper (scientific journal)

Quantification of full and empty particles of adeno-associated virus vectors via a novel dual fluorescence-linked immunosorbent assay.

Sereirath Soth, Mikako Takakura, Masahiro Suekawa, Takayuki Onishi, Kiichi Hirohata, Tamami Hashimoto, Takahiro Maruno, Mitsuko Fukuhara, Yasuo Tsunaka, Tetsuo Torisu, Susumu Uchiyama

Molecular therapy. Methods & clinical development　Vol. 32　No. 3　p. 101291-101291　2024/09/12　Research paper (scientific journal)

Prediction models for the flux decay profile and initial flux of microfiltration for therapeutic proteins

Kota Inoue, Yumiko Masuda, Tetsuo Torisu, Koichi Nonaka, Susumu Uchiyama

Biotechnology and Bioengineering　Vol. 121　No. 6　p. 1889-1901　2024/06　Research paper (scientific journal)

Key role of Pro230 in the hinge region on the IgG architecture and function

Yuuki Koseki, Yuki Yamaguchi, Michihiko Aoyama, Minoru Tada, Akinobu Senoo, Akiko Ishii-Watabe, Takayuki Uchihashi, Susumu Uchiyama, Koichi Kato, Saeko Yanaka, Jose M.M. Caaveiro

2024/05/10

血管・脂肪組織由来分泌因子Favineの血管保護作用機序解明と治療応用

小林 祥子, 福原 充子, 山口 祐希, 加藤 恒, 喜多 俊文, 奥崎 大介, 藤島 裕也, 西澤 恭子, 大月 道夫, 宮下 かずや, 吉田 卓也, 福原 淳範, 森井 英一, 内山 進, 下村 伊一郎

日本内分泌学会雑誌　Vol. 100　No. 1　p. 428-428　2024/05

血管・脂肪組織由来分泌因子Favineの血管保護作用機序解明と治療応用

小林 祥子, 福原 充子, 山口 祐希, 加藤 恒, 喜多 俊文, 奥崎 大介, 藤島 裕也, 西澤 恭子, 大月 道夫, 宮下 かずや, 吉田 卓也, 福原 淳範, 森井 英一, 内山 進, 下村 伊一郎

日本内分泌学会雑誌　Vol. 100　No. 1　p. 428-428　2024/05

Structure of Human Serum Albumin at a Foam Surface

Kanta Enomoto, Tetsuo Torisu, Junya Mizuguchi, Ryosuke Tanoue, Susumu Uchiyama

Journal of Agricultural and Food Chemistry　Vol. 72　No. 15　p. 8774-8783　2024/04/17　Research paper (scientific journal)

Higher-Order Structure of Adeno-Associated Virus Serotype 8 by Hydrogen/Deuterium Exchange Mass Spectrometry

Tomohiko Ikeda, Yuki Yamaguchi, Hiroaki Oyama, Aoba Matsushita, Yasuo Tsunaka, Mitsuko Fukuhara, Tetsuo Torisu, Susumu Uchiyama

Viruses　Vol. 16　No. 4　2024/04/10　Research paper (scientific journal)

Glycosylation of recombinant adeno-associated virus serotype 6

Yuki Yamaguchi, Kentaro Ishii, Sachiko Koizumi, Hiroaki Sakaue, Takahiro Maruno, Mitsuko Fukuhara, Risa Shibuya, Yasuo Tsunaka, Aoba Matsushita, Karin Bandoh, Tetsuo Torisu, Chie Murata-Kishimoto, Azusa Tomioka, Saho Mizukado, Hiroyuki Kaji, Yuji Kashiwakura, Tsukasa Ohmori, Atsushi Kuno, Susumu Uchiyama

Molecular Therapy - Methods & Clinical Development　2024/04　Research paper (scientific journal)

Combined 100 keV cryo-electron microscopy and image analysis methods to characterize the wider adeno-associated viral products.

Haruka Nishiumi, Kiichi Hirohata, Mitsuko Fukuhara, Aoba Matsushita, Yasuo Tsunaka, Mark Allen Vergara Rocafort, Takahiro Maruno, Tetsuo Torisu, Susumu Uchiyama

Journal of pharmaceutical sciences　2024/04/01　Research paper (scientific journal)

Soluble Frizzled-related proteins promote exosome-mediated Wnt re-secretion

Thi Hong Nguyen Tran, Ritsuko Takada, Elena Krayukhina, Takahiro Maruno, Yusuke Mii, Susumu Uchiyama, Shinji Takada

Communications Biology　Vol. 7　No. 1　2024/03/01　Research paper (scientific journal)

Applications and Limitations of Equilibrium Density Gradient Analytical Ultracentrifugation for the Quantitative Characterization of Adeno-Associated Virus Vectors.

Kiichi Hirohata, Yuki Yamaguchi, Takahiro Maruno, Risa Shibuya, Tetsuo Torisu, Takayuki Onishi, Hideto Chono, Junichi Mineno, Yuan Yuzhe, Kiyoko Higashiyama, Kyoko Masumi-Koizumi, Kazuhisa Uchida, Takenori Yamamoto, Eriko Uchida, Takashi Okada, Susumu Uchiyama

Analytical chemistry　2024/01/02　Research paper (scientific journal)

Enhancement of recombinant adeno-associated virus activity by improved stoichiometry and homogeneity of capsid protein assembly.

Takayuki Onishi, Michika Nonaka, Takahiro Maruno, Yuki Yamaguchi, Mitsuko Fukuhara, Tetsuo Torisu, Masaharu Maeda, Susan Abbatiello, Anisha Haris, Keith Richardson, Kevin Giles, Steve Preece, Noriko Yamano-Adachi, Takeshi Omasa, Susumu Uchiyama

Molecular therapy. Methods & clinical development　Vol. 31　p. 101142-101142　2023/12/14　Research paper (scientific journal)

Optimization of Flow Imaging Microscopy Setting Using Spherical Beads with Optical Properties Similar to Those of Biopharmaceuticals.

Takaaki Kurinomaru, Kimitoshi Takeda, Megumi Onaka, Yuki Kuruma, Keiji Takahata, Kayori Takahashi, Hiromu Sakurai, Akira Sasaki, Naohiro Noda, Shinya Honda, Risa Shibuya, Tomohiko Ikeda, Rio Okada, Tetsuo Torisu, Susumu Uchiyama

Journal of pharmaceutical sciences　Vol. 112　No. 12　p. 3248-3255　2023/12　Research paper (scientific journal)

Three-Dimensional Homodyne Light Detection (3D-HLD) for High-Throughput Submicron Particle Analysis in (Highly Concentrated) Protein Biopharmaceuticals, Viral Vectors, and LNPs.

Dominik Brandstetter, Constanze Helbig, Kentaro Osawa, Hiroyuki Minemura, Yumiko Anzai, Tetsuo Torisu, Susumu Uchiyama, Tim Menzen, Wolfgang Friess, Andrea Hawe

Journal of pharmaceutical sciences　2023/11/03　Research paper (scientific journal)

Nicotinamide mononucleotide (NMN) intake increases plasma NMN and insulin levels in healthy subjects.

Takuya Yamane, Momoko Imai, Takeshi Bamba, Susumu Uchiyama

Clinical nutrition ESPEN　Vol. 56　p. 83-86　2023/08　Research paper (scientific journal)

Molecular mechanism underlying the increased risk of colorectal cancer metastasis caused by single nucleotide polymorphisms in LI-cadherin gene.

Anna Yui, Daisuke Kuroda, Takahiro Maruno, Makoto Nakakido, Satoru Nagatoishi, Susumu Uchiyama, Kouhei Tsumoto

Scientific reports　Vol. 13　No. 1　p. 6493-6493　2023/04/20　Research paper (scientific journal)

Critical Calibration of Mass Photometry for Higher-Mass Samples Such as Adeno-Associated Virus Vectors.

Kimitoshi Takeda, Masanori Noda, Takahiro Maruno, Susumu Uchiyama

Journal of pharmaceutical sciences　Vol. 112　No. 4　p. 1145-1150　2023/04　Research paper (scientific journal)

Size Distribution Analysis of the Adeno-Associated Virus Vector by the c(s) Analysis of Band Sedimentation Analytical Ultracentrifugation with Multiwavelength Detection.

Takahiro Maruno, Kentaro Ishii, Tetsuo Torisu, Susumu Uchiyama

Journal of pharmaceutical sciences　Vol. 112　No. 4　p. 937-946　2023/04　Research paper (scientific journal)

Negative interference with antibody-dependent cellular cytotoxicity mediated by rituximab from its interactions with human serum proteins

Saeko Yanaka, Rina Yogo, Hirokazu Yagi, Masayoshi Onitsuka, Natsumi Wakaizumi, Yuki Yamaguchi, Susumu Uchiyama, Koichi Kato

Frontiers in Immunology　Vol. 14　p. 1090898-1090898　2023/01/25　Research paper (scientific journal)

Utility of Three Flow Imaging Microscopy Instruments for Image Analysis in Evaluating four Types of Subvisible Particle in Biopharmaceuticals.

Haruka Nishiumi, Natalie Deiringer, Nils Krause, Saki Yoneda, Tetsuo Torisu, Tim Menzen, Wolfgang Friess, Susumu Uchiyama

Journal of pharmaceutical sciences　Vol. 111　No. 11　p. 3017-3028　2022/11　Research paper (scientific journal)

Structural basis for the toxin-coregulated pilus–dependent secretion of Vibrio cholerae colonization factor

Hiroya Oki, Kazuki Kawahara, Minato Iimori, Yuka Imoto, Haruka Nishiumi, Takahiro Maruno, Susumu Uchiyama, Yuki Muroga, Akihiro Yoshida, Takuya Yoshida, Tadayasu Ohkubo, Shigeaki Matsuda, Tetsuya Iida, Shota Nakamura

Science Advances　Vol. 8　No. 41　2022/10/14　Research paper (scientific journal)

Relationship between aggregation of therapeutic proteins and agitation parameters: Acceleration and frequency.

Shinji Kizuki, Zekun Wang, Tetsuo Torisu, Satoru Yamauchi, Susumu Uchiyama

Journal of pharmaceutical sciences　Vol. 112　No. 2　p. 492-505　2022/09/24　Research paper (scientific journal)

バイオ医薬品のCMC研究開発におけるデジタルトランスフォーメーション(DX)に向けた現状と課題(第3回)[最終回] バイオ医薬品CMC研究開発におけるDigitizationおよびDigitalizationに関する事例紹介

末友 裕行, 柴田 寛子, 高垣 香菜, 山口 祐希, 叶井 正樹, 本山 賢人, 江連 徹, 西風 隆司, 早川 禎宏, 大澤 賢太郎, 桂 真治, 中子 真由美, 馬場 一彦, 丸吉 京介, 三原 和敏, 湯村 恭平, 日向 昌司, 原園 景, 鳥巣 哲生, 内山 進, 石井 明子

PHARM TECH JAPAN　Vol. 38　No. 12　p. 2035-2047　2022/09

バイオ医薬品のCMC研究開発におけるデジタルトランスフォーメーション(DX)に向けた現状と課題(第2回) バイオ医薬品の製法開発や品質評価における分析結果および分析機器のDigitizationおよびDigitalizationの現状

末友 裕行, 大澤 賢太郎, 西風 隆司, 早川 禎宏, 柴田 寛子, 高垣 香菜, 桂 真治, 中子 真由美, 馬場 一彦, 丸吉 京介, 三原 和敏, 湯村 恭平, 日向 昌司, 原園 景, 鳥巣 哲生, 内山 進, 石井 明子

PHARM TECH JAPAN　Vol. 38　No. 11　p. 1825-1830　2022/08

A collaborative study on the classification of silicone oil droplets and protein particles using flow imaging method.

Hiroko Shibata, Masahiro Terabe, Yuriko Shibano, Satoshi Saitoh, Tomohiro Takasugi, Yu Hayashi, Shinji Okabe, Yuka Yamaguchi, Hidehito Yasukawa, Hiroyuki Suetomo, Kazuhiro Miyanabe, Naomi Ohbayashi, Michiko Akimaru, Shuntaro Saito, Daisuke Ito, Atsushi Nakano, Shota Kojima, Yuya Miyahara, Kenji Sasaki, Takahiro Maruno, Masanori Noda, Masato Kiyoshi, Akira Harazono, Tetsuo Torisu, Susumu Uchiyama, Akiko Ishii-Watabe

Journal of pharmaceutical sciences　Vol. 111　No. 10　p. 2745-2757　2022/07/12　Research paper (scientific journal)

バイオ医薬品のCMC研究開発におけるデジタルトランスフォーメーション(DX)に向けた現状と課題(第1回) バイオ医薬品CMC研究開発におけるDigitizationおよびDigitalizationにお関する現状

末友 裕行, 桂 真治, 高垣 香菜, 中子 真由美, 馬場 一彦, 丸吉 京介, 三原 和敏, 湯村 恭平, 日向 昌司, 原園 景, 柴田 寛子, 鳥巣 哲生, 内山 進, 石井 明子

PHARM TECH JAPAN　Vol. 38　No. 10　p. 1599-1605　2022/07

The Fab portion of immunoglobulin G has sites in the CL domain that interact with Fc gamma receptor IIIa

Yuki Yamaguchi, Natsumi Wakaizumi, Mine Irisa, Takahiro Maruno, Mari Shimada, Koya Shintani, Haruka Nishiumi, Rina Yogo, Saeko Yanaka, Daisuke Higo, Tetsuo Torisu, Koichi Kato, Susumu Uchiyama

mAbs　Vol. 14　No. 1　p. 2038531-2038531　2022/03/22　Research paper (scientific journal)

Impacts of Cancer-associated Mutations on the Structure-Activity Relationship of BAP1.

Sarita Puri, Szu-Ni Chen, Yi-Hsiang Chiu, Piotr Draczkowski, Kuang-Ting Ko, Tzu-Jing Yang, Yong-Sheng Wang, Susumu Uchiyama, Shang-Te Danny Hsu

Journal of molecular biology　Vol. 434　No. 9　p. 167553-167553　2022/03/19　Research paper (scientific journal)

Amyloid conformation-dependent disaggregation in a reconstituted yeast prion system.

Yoshiko Nakagawa, Howard C-H Shen, Yusuke Komi, Shinju Sugiyama, Takaaki Kurinomaru, Yuri Tomabechi, Elena Krayukhina, Kenji Okamoto, Takeshi Yokoyama, Mikako Shirouzu, Susumu Uchiyama, Megumi Inaba, Tatsuya Niwa, Yasushi Sako, Hideki Taguchi, Motomasa Tanaka

Nature chemical biology　Vol. 18　No. 3　p. 321-331　2022/02/17　Research paper (scientific journal)

Selective targeting of multiple myeloma cells with a monoclonal antibody recognizing the ubiquitous protein CD98 heavy chain.

Kana Hasegawa, Shunya Ikeda, Moto Yaga, Kouki Watanabe, Rika Urakawa, Akie Iehara, Mai Iwai, Seishin Hashiguchi, Soyoko Morimoto, Fumihiro Fujiki, Hiroko Nakajima, Jun Nakata, Sumiyuki Nishida, Akihiro Tsuboi, Yoshihiro Oka, Satoshi Yoshihara, Masahiro Manabe, Hiroyoshi Ichihara, Atsuko Mugitani, Yasutaka Aoyama, Takafumi Nakao, Asao Hirose, Masayuki Hino, Shiho Ueda, Katsuto Takenaka, Takashi Masuko, Koichi Akashi, Takahiro Maruno, Susumu Uchiyama, Shinji Takamatsu, Naoki Wada, Eiichi Morii, Shushi Nagamori, Daisuke Motooka, Yoshikatsu Kanai, Yusuke Oji, Tomoyoshi Nakagawa, Noriyuki Kijima, Haruhiko Kishima, Atsuyo Ikeda, Takayuki Ogino, Yasushi Shintani, Tateki Kubo, Emiko Mihara, Kosuke Yusa, Haruo Sugiyama, Junichi Takagi, Eiji Miyoshi, Atsushi Kumanogoh, Naoki Hosen

Science translational medicine　Vol. 14　No. 632　2022/02/16　Research paper (scientific journal)

Conformational change in the monomeric alpha-synuclein imparts fibril polymorphs

Cesar Aguirre, Kensuke Ikenaka, Masatomo So, Takahiro Maruno, Keiichi Yamaguchi, Kichitaro Nakajima, Chi-Jing Choong, Kei Nabekura, Goichi Beck, Kentaro Tomii, Yu Yamamori, Junko Doi, Tomoyasu Matsubara, Maho Morishima, Keita Kakuda, Makoto Hideshima, Yasuyoshi Kimura, Seiichi Nagano, Kousuke Baba, Shigeo Murayama, Hirotsugu Ogi, Yoshitaka Nagai, Yasushi Kawata, Susumu Uchiyama, Yohei Miyanoiri, Yuji Goto, Hideki Mochizuki

bioRxiv　2022/02/10　Research paper (scientific journal)

Pro108Ser mutation of SARS-CoV-2 3CLpro reduces the enzyme activity and ameliorates the clinical severity of COVID-19.

Kodai Abe, Yasuaki Kabe, Susumu Uchiyama, Yuka W Iwasaki, Hirotsugu Ishizu, Yoshifumi Uwamino, Toshiki Takenouchi, Shunsuke Uno, Makoto Ishii, Takahiro Maruno, Masanori Noda, Mitsuru Murata, Naoki Hasegawa, Hideyuki Saya, Yuko Kitagawa, Koichi Fukunaga, Masayuki Amagai, Haruhiko Siomi, Makoto Suematsu, Kenjiro Kosaki

Scientific reports　Vol. 12　No. 1　p. 1299-1299　2022/01/25　Research paper (scientific journal)

Estimation of the Viscosity of an Antibody Solution from the Diffusion Interaction Parameter.

Takuya Shimomura, Mitsuhiro Sekiguchi, Reisa Honda, Miki Yamazaki, Masami Yokoyama, Susumu Uchiyama

Biological & pharmaceutical bulletin　Vol. 45　No. 9　p. 1300-1305　2022　Research paper (scientific journal)

Influence of Protein Adsorption on Aggregation in Prefilled Syringes.

Saki Yoneda, Takahiro Maruno, Asuka Mori, Ayana Hioki, Haruka Nishiumi, Rio Okada, Makoto Murakami, Wang Zekun, Ayano Fukuhara, Nozomi Itagaki, Yosuke Harauchi, Satoru Adachi, Kumi Okuyama, Taichi Sawaguchi, Tetsuo Torisu, Susumu Uchiyama

Journal of pharmaceutical sciences　Vol. 110　No. 11　p. 3568-3579　2021/11　Research paper (scientific journal)

Chemical-gas sterilization of external surface of polymer-based prefilled syringes and its effect on stability of model therapeutic protein.

Sho Fujiwara, Tsutomu Ueda, Naoki Ishii, Yoshihiko Abe, Kayoko Hayashihara-Kakuhou, Kimitoshi Takeda, Takaaki Kurinomaru, Ayano Fukuhara, Susumu Uchiyama, John F Carpenter

Journal of pharmaceutical sciences　Vol. 111　No. 1　p. 41-50　2021/09/06　Research paper (scientific journal)

Mechanism of dimerization and structural features of human LI-cadherin

Anna Yui, Jose M.M. Caaveiro, Daisuke Kuroda, Makoto Nakakido, Satoru Nagatoishi, Shuichiro Goda, Takahiro Maruno, Susumu Uchiyama, Kouhei Tsumoto

Journal of Biological Chemistry　Vol. 297　No. 3　p. 101054-101054　2021/09　Research paper (scientific journal)

Plate Reader-Based Analytical Method for the Size Distribution of Submicron-Sized Protein Aggregates Using Three-Dimensional Homodyne Light Detection.

Ayano Fukuhara, Yumiko Anzai, Kentaro Osawa, Mariko Umeda, Hiroyuki Minemura, Nobuhiro Shiramizu, Masami Yokoyama, Susumu Uchiyama

Journal of pharmaceutical sciences　Vol. 110　No. 12　p. 3803-3810　2021/08/20　Research paper (scientific journal)

Characterization of Adeno-Associated Virus Capsid Proteins with Two Types of VP3-Related Components by Capillary Gel Electrophoresis and Mass Spectrometry.

Hiroaki Oyama, Kentaro Ishii, Takahiro Maruno, Tetsuo Torisu, Susumu Uchiyama

Human gene therapy　Vol. 32　No. 21-22　p. 1403-1416　2021/07/16　Research paper (scientific journal)

Glycyrrhizin derivatives suppress cancer chemoresistance by inhibiting progesterone receptor membrane component 1

Yasuaki Kabe, Ikko Koike, Tatsuya Yamamoto, Miwa Hirai, Ayaka Kanai, Ryogo Furuhata, Hitoshi Tsugawa, Erisa Harada, Kenji Sugase, Kazue Hanadate, Nobuji Yoshikawa, Hiroaki Hayashi, Masanori Noda, Susumu Uchiyama, Hiroki Yamazaki, Hirotoshi Tanaka, Takuya Kobayashi, Hiroshi Handa, Makoto Suematsu

Cancers　Vol. 13　No. 13　2021/07/01　Research paper (scientific journal)

Comprehensive size distribution and composition analysis of adeno-associated virus vector by multiwavelength sedimentation velocity analytical ultracentrifugation.

Takahiro Maruno, Kaede Usami, Kentaro Ishii, Tetsuo Torisu, Susumu Uchiyama

Journal of pharmaceutical sciences　Vol. 110　No. 10　p. 3375-3384　2021/06/26　Research paper (scientific journal)

Physicochemical Characterization of Sabin Inactivated Poliovirus Vaccine for Process Development

Tetsuo Torisu, Saori Shikama, Kuniaki Nakamura, Kanta Enomoto, Takahiro Maruno, Asuka Mori, Susumu Uchiyama, Tatsuki Satou

Journal of Pharmaceutical Sciences　Vol. 110　No. 5　p. 2121-2129　2021/05　Research paper (scientific journal)

A Multi-Method Approach to Assess the Self-Interaction Behavior of Infliximab

Martin Domnowski, Takahiro Maruno, Kanta Enomoto, Felix Kummer, Alina Kulakova, Pernille Harris, Susumu Uchiyama, Jan Jaehrling, Wolfgang Friess

Journal of Pharmaceutical Sciences　Vol. 110　No. 5　p. 1979-1988　2021/05　Research paper (scientific journal)

Reduction of Recombinant Adeno-Associated Virus Vector Adsorption on Solid Surfaces by Polyionic Hydrophilic Complex Coating

Salama Ramy, Yuki Ueda, Hiroyuki Nakajima, Miya Hiroi, Yoshiomi Hiroi, Tetsuo Torisu, Susumu Uchiyama

Journal of Pharmaceutical Sciences　Vol. 111　No. 3　p. 663-671　2021/03　Research paper (scientific journal)

Critical analysis of techniques and materials used in devices, syringes, and needles used for intravitreal injections

Gustavo Barreto Melo, Natasha Ferreira Santos da Cruz, Geoffrey Guy Emerson, Flávio Atanázio Rezende, Carsten H. Meyer, Susumu Uchiyama, John Carpenter, Hélio Francisco Shiroma, Michel Eid Farah, Maurício Maia, Eduardo Büchele Rodrigues

Progress in Retinal and Eye Research　Vol. 80　p. 100862-100862　2021/01　Research paper (scientific journal)

An influenza HA stalk reactive polymeric IgA antibody exhibits anti-viral function regulated by binary interaction between HA and the antibody

Kaori Sano, Shinji Saito, Tadaki Suzuki, Osamu Kotani, Akira Ainai, Elly van Riet, Koshiro Tabata, Kumpei Saito, Yoshimasa Takahashi, Masaru Yokoyama, Hironori Sato, Takahiro Maruno, Kaede Usami, Susumu Uchiyama, Kiyoko Ogawa-Goto, Hideki Hasegawa

PLoS ONE　Vol. 16　No. 1 January　2021/01　Research paper (scientific journal)

Development of syringes and vials for delivery of biologics: current challenges and innovative solutions

Saki Yoneda, Tetsuo Torisu, Susumu Uchiyama

Expert Opinion on Drug Delivery　Vol. 18　No. 4　p. 459-470　2021/01　Research paper (scientific journal)

Intrastrand backbone-nucleobase interactions stabilize unwound right-handed helical structures of heteroduplexes of L-aTNA/RNA and SNA/RNA

Yukiko Kamiya, Tadashi Satoh, Atsuji Kodama, Tatsuya Suzuki, Keiji Murayama, Hiromu Kashida, Susumu Uchiyama, Koichi Kato, Hiroyuki Asanuma

Communications Chemistry　Vol. 3　No. 1　2020/12/01　Research paper (scientific journal)

Effect of UVC Irradiation on the Oxidation of Histidine in Monoclonal Antibodies

Yuya Miyahara, Koya Shintani, Kayoko Hayashihara-Kakuhou, Takehiro Zukawa, Yukihiro Morita, Takashi Nakazawa, Takuya Yoshida, Tadayasu Ohkubo, Susumu Uchiyama

Scientific Reports　Vol. 10　No. 1　p. 6333-6333　2020/12/01　Research paper (scientific journal)

Allosteric regulation accompanied by oligomeric state changes of Trypanosoma brucei GMP reductase through cystathionine-β-synthase domain

Akira Imamura, Tetsuya Okada, Hikaru Mase, Takuya Otani, Tomoka Kobayashi, Manatsu Tamura, Bruno Kilunga Kubata, Katsuaki Inoue, Robert P. Rambo, Susumu Uchiyama, Kentaro Ishii, Shigenori Nishimura, Takashi Inui

Nature Communications　Vol. 11　No. 1　p. 1837-1837　2020/12/01　Research paper (scientific journal)

Supramolecular tholos-like architecture constituted by archaeal proteins without functional annotation

Maho Yagi-Utsumi, Arunima Sikdar, Chihong Song, Jimin Park, Rintaro Inoue, Hiroki Watanabe, Raymond N. Burton-Smith, Toshiya Kozai, Tatsuya Suzuki, Atsuji Kodama, Kentaro Ishii, Hirokazu Yagi, Tadashi Satoh, Susumu Uchiyama, Takayuki Uchihashi, Keehyoung Joo, Jooyoung Lee, Masaaki Sugiyama, Kazuyoshi Murata, Koichi Kato

Scientific Reports　Vol. 10　No. 1　p. 1540-1540　2020/12/01　Research paper (scientific journal)

Pro108Ser mutant of SARS-CoV-2 3CLpro reduces the enzymatic activity and ameliorates COVID-19 severity in Japan

Kodai Abe, Yasuaki Kabe, Susumu Uchiyama, Yuka Iwasaki W., Hirotsugu Ishizu, Yoshifumi Uwamino, Toshiki Takenouchi, Shunsuke Uno, Makoto Ishii, Maruno Takahiro, Noda Masanori, Mitsuru Murata, Naoki Hasegawa, Hideyuki Saya, Yuko Kitagawa, Koichi Fukunaga, Masayuki Amagai, Haruhiko Siomi, Makoto Suematsu, Kenjiro Kosaki

medRxiv　2020/11/24

Recent Achievements and Current Interests in Research on the Characterization and Quality Control of Biopharmaceuticals in Japan

Akiko Ishii-Watabe, Hiroko Shibata, Hiroyuki Suetomo, Yosuke Ikeda, Srivalli Telikepalli, Masato Kiyoshi, Yu Hayashi, Takashi Muto, Yukako Tanaka, Satomi Ueda, Takafumi Iwura, Satoshi Saitoh, Michihiko Aoyama, Akira Harazono, Masashi Hyuga, Yukihiro Goda, Tetsuo Torisu, Susumu Uchiyama

Journal of Pharmaceutical Sciences　Vol. 109　No. 5　p. 1652-1661　2020/05

Dataset of microbial community structure in alcohol sprayed banana associated with ripening process

Fenny Martha Dwivany, Fidya Syam, Husna Nugrahapraja, Ocky Karna Radjasa, Maelita Ramdani Moeis, Susumu Uchiyama

Data in Brief　Vol. 29　p. 105216-105216　2020/04　Research paper (scientific journal)

Current status and issues of protein solution biophysics—Session 1SDP

Saeko Yanaka, Susumu Uchiyama

Biophysical Reviews　Vol. 12　No. 2　p. 263-264　2020/04

Incorporation of Pseudo-complementary Bases 2,6-Diaminopurine and 2-Thiouracil into Serinol Nucleic Acid (SNA) to Promote SNA/RNA Hybridization

Yukiko Kamiya, Fuminori Sato, Keiji Murayama, Atsuji Kodama, Susumu Uchiyama, Hiroyuki Asanuma

Chemistry - An Asian Journal　Vol. 15　No. 8　p. 1266-1271　2020/04　Research paper (scientific journal)

Stirring rate affects thermodynamics and unfolding kinetics in isothermal titration calorimetry

Takahiro Maruno, Tadayasu Ohkubo, Susumu Uchiyama

Journal of Biochemistry　Vol. 168　No. 1　p. 53-62　2020　Research paper (scientific journal)

Assessment of the Injection Performance of a Tapered Needle for Use in Prefilled Biopharmaceutical Products

Elena Krayukhina, Ayano Fukuhara, Susumu Uchiyama

Journal of Pharmaceutical Sciences　Vol. 109　No. 1　p. 515-523　2020/01　Research paper (scientific journal)

Relation of Colloidal and Conformational Stabilities to Aggregate Formation in a Monoclonal Antibody

Hiroaki Oyama, Hiroki Koga, Takashi Tadokoro, Katsumi Maenaka, Akira Shiota, Masami Yokoyama, Masanori Noda, Tetsuo Torisu, Susumu Uchiyama

Journal of Pharmaceutical Sciences　Vol. 109　No. 1　p. 308-315　2020/01　Research paper (scientific journal)

Automatic Identification of the Stress Sources of Protein Aggregates Using Flow Imaging Microscopy Images

Arni Gambe-Gilbuena, Yuriko Shibano, Elena Krayukhina, Tetsuo Torisu, Susumu Uchiyama

Journal of Pharmaceutical Sciences　Vol. 109　No. 1　p. 614-623　2020/01　Research paper (scientific journal)

Polarizability and isotope effects on dispersion interactions in water

Yi Yang Zhan, Qi Chun Jiang, Kentaro Ishii, Takuya Koide, Osamu Kobayashi, Tatsuo Kojima, Satoshi Takahashi, Masanori Tachikawa, Susumu Uchiyama, Shuichi Hiraoka

Communications Chemistry　Vol. 2　No. 1　2019/12/01　Research paper (scientific journal)

Structural characterization of HypX responsible for CO biosynthesis in the maturation of NiFe-hydrogenase

Norifumi Muraki, Kentaro Ishii, Susumu Uchiyama, Satoru G. Itoh, Hisashi Okumura, Shigetoshi Aono

Communications Biology　Vol. 2　No. 1　p. 385-385　2019/12/01　Research paper (scientific journal)

A head-to-toe dimerization has physiological relevance for ligand-induced inactivation of protein tyrosine receptor type Z.

Akihiro Fujikawa, Hajime Sugawara, Naomi Tanga, Kentaro Ishii, Kazuya Kuboyama, Susumu Uchiyama, Ryoko Suzuki, Masaharu Noda

The Journal of biological chemistry　Vol. 294　No. 41　p. 14953-14965　2019/10/11　Research paper (scientific journal)

Cooperative Binding of KaiB to the KaiC Hexamer Ensures Accurate Circadian Clock Oscillation in Cyanobacteria.

Reiko Murakami, Yasuhiro Yunoki, Kentaro Ishii, Kazuki Terauchi, Susumu Uchiyama, Hirokazu Yagi, Koichi Kato

International journal of molecular sciences　Vol. 20　No. 18　2019/09/13　Research paper (scientific journal)

Inhalation effect of massoilactone from massoia essential oil on lipid profile, liver tissues, and body weight of Sprague Dawley rat

Irmanida Batubara, Yanico Hadi Prayogo, Irma Herawati Suparto, Berry Juliandi, Susumu Uchiyama

Journal of Applied Pharmaceutical Science　Vol. 9　No. 8　p. 111-116　2019/08/01　Research paper (scientific journal)

The Fab portion of immunoglobulin G contributes to its binding to Fc gamma receptor III

Rina Yogo, Yuki Yamaguchi, Hiroki Watanabe, Hirokazu Yagi, Tadashi Satoh, Mahito Nakanishi, Masayoshi Onitsuka, Takeshi Omasa, Mari Shimada, Takahiro Maruno, Tetsuo Torisu, Shio Watanabe, Daisuke Higo, Takayuki Uchihashi, Saeko Yanaka, Susumu Uchiyama, Koichi Kato

SCIENTIFIC REPORTS　Vol. 9　No. 1　p. 11957-11957　2019/08　Research paper (scientific journal)

Publisher Correction: Dynamic structural states of ClpB involved in its disaggregation function.

Takayuki Uchihashi, Yo-Hei Watanabe, Yosuke Nakazaki, Takashi Yamasaki, Hiroki Watanabe, Takahiro Maruno, Kentaro Ishii, Susumu Uchiyama, Chihong Song, Kazuyoshi Murata, Ryota Iino, Toshio Ando

Nature communications　Vol. 10　No. 1　p. 3079-3079　2019/07/12　Research paper (scientific journal)

An Assessment of the Ability of Submicron- and Micron-Size Silicone Oil Droplets in Dropped Prefillable Syringes to Invoke Early- and Late-Stage Immune Responses.

Elena Krayukhina, Masami Yokoyama, Kayoko Kakuhou Hayashihara, Takahiro Maruno, Masanori Noda, Hiroki Watanabe, Takayuki Uchihashi, Susumu Uchiyama

Journal of pharmaceutical sciences　Vol. 108　No. 7　p. 2278-2287　2019/07　Research paper (scientific journal)

Identification of IgG1 Aggregation Initiation Region by Hydrogen Deuterium Mass Spectrometry.

Masanori Noda, Kentaro Ishii, Mika Yamauchi, Hiroaki Oyama, Takashi Tadokoro, Katsumi Maenaka, Tetsuo Torisu, Susumu Uchiyama

Journal of pharmaceutical sciences　Vol. 108　No. 7　p. 2323-2333　2019/07　Research paper (scientific journal)

ATP hydrolysis by KaiC promotes its KaiA binding in the cyanobacterial circadian clock system

Yasuhiro Yunoki, Kentaro Ishii, Maho Yagi-Utsumi, Reiko Murakami, Susumu Uchiyama, Hirokazu Yagi, Koichi Kato

LIFE SCIENCE ALLIANCE　Vol. 2　No. 3　2019/06　Research paper (scientific journal)

Mutational and Combinatorial Control of Self-Assembling and Disassembling of Human Proteasome α Subunits.

Taichiro Sekiguchi, Tadashi Satoh, Eiji Kurimoto, Chihong Song, Toshiya Kozai, Hiroki Watanabe, Kentaro Ishii, Hirokazu Yagi, Saeko Yanaka, Susumu Uchiyama, Takayuki Uchihashi, Kazuyoshi Murata, Koichi Kato

International journal of molecular sciences　Vol. 20　No. 9　2019/05/09　Research paper (scientific journal)

Biophysical characterization and smart formulation of autibeody drug

Susumu Uchiyama

Japanese Journal of Clinical Chemistry　Vol. 48　No. 2　p. 108-113　2019/04　Research paper (scientific journal)

バイオ医薬品における凝集とスマートフォーミュレーション

内山 進

臨床化学　Vol. 2　No. 2　2019/04

Ionic liquids and protein folding-old tricks for new solvents.

Ryota Wakayama, Susumu Uchiyama, Damien Hall

Biophysical reviews　Vol. 11　No. 2　p. 209-225　2019/04

Temperature-controlled repeatable scrambling and induced-sorting of building blocks between cubic assemblies

Yi-Yang Zhan, Tatsuo Kojima, Kentaro Ishii, Satoshi Takahashi, Yohei Haketa, Hiromitsu Maeda, Susumu Uchiyama, Shuichi Hiraoka

NATURE COMMUNICATIONS　Vol. 10　No. 1　p. 1440-1440　2019/03　Research paper (scientific journal)

SDS-induced oligomerization of Lys49-phospholipase A2 from snake venom.

Takashi Matsui, Shizuka Kamata, Kentaro Ishii, Takahiro Maruno, Nouran Ghanem, Susumu Uchiyama, Koichi Kato, Atsuo Suzuki, Naoko Oda-Ueda, Tomohisa Ogawa, Yoshikazu Tanaka

Scientific reports　Vol. 9　No. 1　p. 2330-2330　2019/02/20　Research paper (scientific journal)

Crystal structure of the dog allergen Can f 6 and structure-based implications of its cross-reactivity with the cat allergen Fel d 4

Kenji Yamamoto, Osamu Ishibashi, Keisuke Sugiura, Miki Ubatani, Masaya Sakaguchi, Masatoshi Nakatsuji, Shigeru Shimamoto, Masanori Noda, Susumu Uchiyama, Yuma Fukutomi, Shigenori Nishimura, Takashi Inui

SCIENTIFIC REPORTS　Vol. 9　No. 1　p. 1503-1503　2019/02　Research paper (scientific journal)

Aggregates quantification of biopharmaceuticals in a wide range of sizes using orthogonal methods

Susumu Uchiyama

American Pharmaceutical Review　Vol. 22　No. 4　2019　Research paper (scientific journal)

高品質で安全な抗体医薬のための高度分析

内山 進

Vol. 71　No. 1　2019/01　Research paper (bulletin of university, research institution)

Quantitative Laser Diffraction for Quantification of Protein Aggregates: Comparison With Resonant Mass Measurement, Nanoparticle Tracking Analysis, Flow Imaging, and Light Obscuration

Saki Yoneda, Bertram Niederleitner, Michael Wiggenhorn, Hiroki Koga, Shinichiro Totoki, Elena Krayukhina, Wolfgang Friess, Susumu Uchiyama

JOURNAL OF PHARMACEUTICAL SCIENCES　Vol. 108　No. 1　p. 755-762　2019/01　Research paper (scientific journal)

[Current Trends in Analytical Technologies Which Promote the Development of Biopharmaceuticals].

Ishii-Watabe A, Uchiyama S

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan　Vol. 138　No. 12　p. 1473-1474　2018/12

[Toward the Proper Biophysical Characterization of Aggregates in Biopharmaceuticals].

Uchiyama S

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan　Vol. 138　No. 12　p. 1503-1507　2018/12

Native MS Ionization Process and Non-Covalent Interactions of Protein Complex

Ishii Kentaro, Uchiyama Susumu

Journal of the Mass Spectrometry Society of Japan　Vol. 66　No. 6　p. 222-225　2018/12

Analysis of Higher Order Structures of Proteins by Hydrogen Deuterium Exchange Mass Spectrometry

Torisu Tetsuo, Oyama Hiroaki, Uchiyama Susumu

Journal of the Mass Spectrometry Society of Japan　Vol. 66　No. 6　p. 219-221　2018/12

Preface: Issue “Protein Mass Spectrometry”

Uchiyama Susumu

Journal of the Mass Spectrometry Society of Japan　Vol. 66　No. 6　p. 209-209　2018/12

Interlaboratory comparison about feasibility of insoluble particulate matter test for injections with reduced test volume in light obscuration method.

Harazono Akira, Shibata Hiroko, Kiyoshi Masato, Muto Takashi, Fukud Jun, Torisu Tetsuo, Saitoh Satoshi, Nishimura Hirotaka, Uchiyama Susumu, Ishii-Watabe Akiko

Biologicals : journal of the International Association of Biological Standardization　Vol. 57　p. 46-49　2018/12　Research paper (scientific journal)

抗体医薬品開発における質量分析技術

尾山博章, 内山 進

SAR News　Vol. 35　p. 8-15　2018/10

Structural Dynamics of the PET-Degrading Cutinase-like Enzyme from Saccharomonospora viridis AHK190 in Substrate-Bound States Elucidates the Ca2+-Driven Catalytic Cycle

Nobutaka Numoto, Narutoshi Kamiya, Gert-Jan Bekker, Yuri Yamagami, Satomi Inaba, Kentaro Ishii, Susumu Uchiyama, Fusako Kawai, Nobutoshi Ito, Masayuki Oda

Biochemistry　Vol. 57　No. 36　p. 5289-5300　2018/09/11　Research paper (scientific journal)

Collaborative Study for Analysis of Subvisible Particles Using Flow Imaging and Light Obscuration: Experiences in Japanese Biopharmaceutical Consortium.

Kiyoshi Masato, Shibata Hiroko, Harazono Akira, Torisu Tetsuo, Maruno Takahiro, Akimaru Michiko, Asano Yuuka, Hirokawa Mai, Ikemoto Keisuke, Itakura Yukari, Iwura Takafumi, Kikitsu Aya, Kumagai Takashi, Mori Naoki, Murase Hiroaki, Nishimura Hirotaka, Oda Atsushi, Ogawa Taiichiro, Ojima Takuma, Okabe Shinji, Saito Shuntaro, Saitoh Satoshi, Suetomo Hiroyuki, Takegami Kazuhiro, Takeuchi Momoko, Yasukawa Hidehito, Uchiyama Susumu, Ishii-Watabe Akiko

Journal of pharmaceutical sciences　Vol. 108　No. 2　p. 832-841　2018/08　Research paper (scientific journal)

Dynamic structural states of CIpB involved in its disaggregation function

Takayuki Uchihashi, Yo-hei Watanabe, Yosuke Nakazaki, Takashi Yamasaki, Hiroki Watanabe, Takahiro Maruno, Kentaro Ishii, Susumu Uchiyama, Chihong Song, Kazuyoshi Murata, Ryota Iino, Toshio Ando

NATURE COMMUNICATIONS　Vol. 9　No. 1　2018/06　Research paper (scientific journal)

Sweeping of Adsorbed Therapeutic Protein on Prefillable Syringes Promotes Micron Aggregate Generation

Takahiro Maruno, Hiroki Watanabe, Saki Yoneda, Takayuki Uchihashi, Satoru Adachi, Kunihito Arai, Taichi Sawaguchi, Susumu Uchiyama

Journal of Pharmaceutical Sciences　Vol. 107　No. 6　p. 1521-1529　2018/06/01　Research paper (scientific journal)

Physicochemical improvement of rabbit derived single-domain antibodies by substitutions with amino acids conserved in camelid antibodies

Naoya Shinozaki, Ryuji Hashimoto, Masanori Noda, Susumu Uchiyama

Journal of Bioscience and Bioengineering　Vol. 125　No. 6　p. 654-661　2018/06/01　Research paper (scientific journal)

Bifacial Nucleobases for Hexaplex Formation in Aqueous Solution.

Kashida Hiromu, Hattori Yuhei, Tazoe Kaho, Inoue Tadashi, Nishikawa Keiji, Ishii Kentaro, Uchiyama Susumu, Yamashita Hayato, Abe Masayuki, Kamiya Yukiko, Asanuma Hiroyuki

Journal of the American Chemical Society　Vol. 140　No. 27　p. 8456-8462　2018/06　Research paper (scientific journal)

バイオ医薬の分析のコツ品質評価のための基礎と応用：第10回タンパク質の凝集と凝集体評価

クラユヒナ エレナ, 米田早紀, 古賀博己, 丸野孝浩, 内山 進

ファームテックジャパン　Vol. 34　No. 7　2018/05

Sedimentation velocity analytical ultracentrifugation for characterization of therapeutic antibodies

Susumu Uchiyama, Masanori Noda, Elena Krayukhina

Biophysical Reviews　Vol. 10　No. 2　p. 259-269　2018/04/01

RecA requires two molecules of Mg2+ ions for its optimal strand exchange activity in vitro

Kim Raeyeong, Kanamaru Shuji, Mikawa Tsutomu, Prevost Chantal, Ishii Kentaro, Ito Kentaro, Uchiyama Susumu, Oda Masayuki, Iwasaki Hiroshi, Kim Seog K, Takahashi Masayuki

Nucleic Acids Research　Vol. 46　No. 5　p. 2548-2559　2018/03/16　Research paper (scientific journal)

Hyperthermostable cube-shaped assembly in water

Yi-Yang Zhan, Kazuho Ogata, Tatsuo Kojima, Takuya Koide, Kentaro Ishii, Takako Mashiko, Masanori Tachikawa, Susumu Uchiyama, Shuichi Hiraoka

COMMUNICATIONS CHEMISTRY　Vol. 1　No. 1　2018/03　Research paper (scientific journal)

Native mass spectrometry for understanding dynamic protein complex

Kentaro Ishii, Min Zhou, Susumu Uchiyama

Biochimica et Biophysica Acta - General Subjects　Vol. 1862　No. 2　p. 275-286　2018/02/01

Insight into adaptive remodeling of the rotor ring complex of the bacterial flagellar motor

Miki Kinoshita, Yukio Furukawa, Susumu Uchiyama, Katsumi Imada, Keiichi Namba, Tohru Minamino

Biochemical and Biophysical Research Communications　Vol. 496　No. 1　p. 12-17　2018/01/29　Research paper (scientific journal)

バイオ医薬の分析のコツ品質評価のための基礎と応用：第6回タンパク質溶液の性質

内山 進

ファームテックジャパン　Vol. 34　No. 1　2018/01

Recent Developments of Cyclo Olefin Polymers

Harauchi Yosuke, Houkawa Takashi, Adachi Satoru, Arai Kunihito, Sawaguchi Taichi, Hayano Shigetaka, Uchiyama Susumu

KOBUNSHI RONBUNSHU　Vol. 75　No. 6　p. 477-485　2018

Assembly of protein complexes restricts diffusion of Wnt3a proteins.

Ritsuko Takada, Yusuke Mii, Elena Krayukhina, Yuusuke Maruyama, Kazuhiro Mio, Yoshikazu Sasaki, Takao Shinkawa, Chan-Gi Pack, Yasushi Sako, Chikara Sato, Susumu Uchiyama, Shinji Takada

Communications biology　Vol. 1　No. 1　p. 165-165　2018　Research paper (scientific journal)

Recent Topics of Research in the Characterization and Quality Control of Biopharmaceuticals in Japan

Akiko Ishii-Watabe, Hiroko Shibata, Akira Harazono, Masashi Hyuga, Masato Kiyoshi, Satoshi Saitoh, Takafumi Iwura, Tetsuo Torisu, Yukihiro Goda, Susumu Uchiyama

JOURNAL OF PHARMACEUTICAL SCIENCES　Vol. 106　No. 12　p. 3431-3437　2017/12

A Comprehensive Study of the Interaction between Peptidoglycan Fragments and the Extracellular Domain of Mycobacterium tuberculosis Ser/Thr Kinase PknB

Qianqian Wang, Roberta Marchetti, Sladjana Prisic, Kentaro Ishii, Yohei Arai, Ippei Ohta, Shinsuke Inuki, Susumu Uchiyama, Alba Silipo, Antonio Molinaro, Robert N. Husson, Koichi Fukase, Yukari Fujimoto

CHEMBIOCHEM　Vol. 18　No. 21　p. 2094-2098　2017/11　Research paper (scientific journal)

バイオ医薬の分析のコツ品質評価のための基礎と応用：第3回タンパク質の構造を知る

林原加代子, 宮原佑弥, 丸野孝浩, 野田勝紀, 内山 進

ファームテックジャパン　Vol. 34　No. 33　2017/10

NMR Detection of Semi-Specific Antibody Interactions in Serum Environments

Saeko Yanaka, Toshio Yamazaki, Rina Yogo, Masanori Noda, Susumu Uchiyama, Hirokazu Yagi, Koichi Kato

MOLECULES　Vol. 22　No. 10　2017/10　Research paper (scientific journal)

Friability Testing as a New Stress-Stability Assay for Biopharmaceuticals

Tetsuo Torisu, Takahiro Maruno, Saki Yoneda, Yoshinori Hamaji, Shinya Honda, Tadayasu Ohkubo, Susumu Uchiyama

JOURNAL OF PHARMACEUTICAL SCIENCES　Vol. 106　No. 10　p. 2966-2978　2017/10　Research paper (scientific journal)

Calcium depletion destabilises kinetochore fibres by the removal of CENP-F from the kinetochore

Rinyaporn Phengchat, Hideaki Takata, Susumu Uchiyama, Kiichi Fukui

SCIENTIFIC REPORTS　Vol. 7　No. 1　2017/08　Research paper (scientific journal)

蛋白質溶液製剤の安定性評価と最適化

内山 進

ケミカルエンジニヤリング　Vol. 8　p. 1-6　2017/08

Interdependency and phosphorylation of KIF4 and condensin I are essential for organization of chromosome scaffold

Rawin Poonperm, Hideaki Takata, Susumu Uchiyama, Kiichi Fukui

PLOS ONE　Vol. 12　No. 8　2017/08　Research paper (scientific journal)

Efficient generation of single domain antibodies with high affinities and enhanced thermal stabilities

Naoya Shinozaki, Ryuji Hashimoto, Kiichi Fukui, Susumu Uchiyama

SCIENTIFIC REPORTS　Vol. 7　No. 1　2017/07　Research paper (scientific journal)

タンパク質のネイティブ質量分析—動的なタンパク質複合体形成の解明に向けて—

石井健太郎, 内山 進

ぶんせき　Vol. 10　p. 472-475　2017/07

Novel helical assembly in arginine methyltransferase 8

S. Toma-Fukai, J-D. Kim, K-E. Park, N. Kuwabara, N. Shimizu, E. Krayukuhina, S. Uchiyama, A. Fukamizu, T. Shimizu

EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS　Vol. 46　p. S178-S178　2017/07

バイオ医薬品の物理化学分析の重要性と必要性より安全な価値の高いタンパク質製剤の開発にむけて

内山 進

ファームテックジャパン　Vol. 33　No. 5　p. 89-94　2017/04

Synergistic Effect of Cavitation and Agitation on Protein Aggregation

Tetsuo Torisu, Takahiro Maruno, Yoshinori Hamaji, Tadayasu Ohkubo, Susumu Uchiyama

JOURNAL OF PHARMACEUTICAL SCIENCES　Vol. 106　No. 2　p. 521-529　2017/02　Research paper (scientific journal)

Continuing Bioengineering basic course - Bio storytelling - Bio s

Uchiyama Susumu

Seibutsu-kogaku Kaishi　Vol. 95　No. 5　p. 262-267　2017　Research paper (scientific journal)

Establishment of cell lines from adult T-cell leukemia cells dependent on negatively charged polymers

Kagami Yoshitoyo, Uchiyama Susumu, Kato Harumi, Okada Yasutaka, Seto Masao, Kinoshita Tomohiro

Journal of Clinical and Experimental Hematopathology　Vol. 57　No. 1　p. 9-14　2017

Analytical ultracentrifugation with fluorescence detection system reveals differences in complex formation between recombinant human TNF and different biological TNF antagonists in various environments

Elena Krayukhina, Masanori Noda, Kentaro Ishii, Takahiro Maruno, Hirotsugu Wakabayashi, Minoru Tada, Takuo Suzuki, Akiko Ishii-Watabe, Masahiko Kato, Susumu Uchiyama

MABS　Vol. 9　No. 4　p. 664-679　2017　Research paper (scientific journal)

The effect of magnesium ions on chromosome structure as observed by scanning electron microscopy (SEM) and scanning transmission electron microscope (STEM) tomography

Dwiranti, Astari, Takata, Hideaki, Uchiyama, Susumu, Fukui, Kiichi

Chromosome Sci.　Vol. 19　No. 1-4　p. 19-23　2016/12

Calcium ions function as a booster of chromosome condensation

Rinyaporn Phengchat, Hideaki Takata, Kenichi Morii, Noriko Inada, Hideji Murakoshi, Susumu Uchiyama, Kiichi Fukui

SCIENTIFIC REPORTS　Vol. 6　2016/12　Research paper (scientific journal)

Taste substance binding to the ligand-binding domains of T1r taste receptor heterodimer

Atsuko Yamashita, Eriko Nango, Shuji Akiyama, Saori Maki-Yonekura, Yuji Ashikawa, Yuko Kusakabe, Elena Krayukhina, Takahiro Maruno, Susumu Uchiyama, Nipawan Nuemket, Koji Yonekura, Madoka Shimizu, Nanako Atsumi, Norihisa Yasui, Takaaki Hikima, Masaki Yamamoto, Yuji Kobayashi

CHEMICAL SENSES　Vol. 41　No. 9　p. E176-E177　2016/11

Interaction mode between catalytic and regulatory subunits in glucosidase II involved in ER glycoprotein quality control

Tadashi Satoh, Takayasu Toshimori, Masanori Noda, Susumu Uchiyama, Koichi Kato

PROTEIN SCIENCE　Vol. 25　No. 11　p. 2095-2101　2016/11　Research paper (scientific journal)

Structural characterization of the circadian clock protein complex composed of KaiB and KaiC by inverse contrast-matching small-angle neutron scattering.

Masaaki Sugiyama, Hirokazu Yagi, Kentaro Ishii, Lionel Porcar, Anne Martel, Katsuaki Oyama, Masanori Noda, Yasuhiro Yunoki, Reiko Murakami, Rintaro Inoue, Nobuhiro Sato, Yojiro Oba, Kazuki Terauchi, Susumu Uchiyama, Koichi Kato

Scientific reports　Vol. 6　p. 35567-35567　2016/10/18　Research paper (scientific journal)

Structural Analysis Reveals that Toll-like Receptor 7 Is a Dual Receptor for Guanosine and Single-Stranded RNA

Zhikuan Zhang, Umeharu Ohto, Takuma Shibata, Elena Krayukhina, Masato Taoka, Yoshio Yamauchi, Hiromi Tanji, Toshiaki Isobe, Susumu Uchiyama, Kensuke Miyake, Toshiyuki Shimizu

IMMUNITY　Vol. 45　No. 4　p. 737-748　2016/10　Research paper (scientific journal)

Structural and binding properties of laminarin revealed by analytical ultracentrifugation and calorimetric analyses

Masayuki Oda, Yoichi Tanabe, Masanori Noda, Satomi Inaba, Elena Krayukhina, Harumi Fukada, Susumu Uchiyama

CARBOHYDRATE RESEARCH　Vol. 431　p. 33-38　2016/08　Research paper (scientific journal)

3D-Printing for Analytical Ultracentrifugation

Abhiksha Desai, Jonathan Krynitsky, Thomas J. Pohida, Huaying Zhao, Peter Schuck

PLOS ONE　Vol. 11　No. 8　p. 165-183　2016/08　Research paper (scientific journal)

New insight into the dynamical system of alpha B-crystallin oligomers

Rintaro Inoue, Takumi Takata, Norihiko Fujii, Kentaro Ishii, Susumu Uchiyama, Nobuhiro Sato, Yojiro Oba, Kathleen Wood, Koichi Kato, Noriko Fujii, Masaaki Sugiyama

SCIENTIFIC REPORTS　Vol. 6　2016/07　Research paper (scientific journal)

Structure of IZUMO1-JUNO reveals sperm-oocyte recognition during mammalian fertilization

Umeharu Ohto, Hanako Ishida, Elena Krayukhina, Susumu Uchiyama, Naokazu Inoue, Toshiyuki Shimizu

NATURE　Vol. 534　No. 7608　p. 566-+　2016/06　Research paper (scientific journal)

Taste substance binding elicits conformational change of taste receptor T1r heterodimer extracellular domains

Eriko Nango, Shuji Akiyama, Saori Maki-Yonekura, Yuji Ashikawa, Yuko Kusakabe, Elena Krayukhina, Takahiro Maruno, Susumu Uchiyama, Nipawan Nuemket, Koji Yonekura, Madoka Shimizu, Nanako Atsumi, Norihisa Yasui, Takaaki Hikima, Masaki Yamamoto, Yuji Kobayashi, Atsuko Yamashita

SCIENTIFIC REPORTS　Vol. 6　2016/05　Research paper (scientific journal)

Haem-dependent dimerization of PGRMC1/Sigma-2 receptor facilitates cancer proliferation and chemoresistance

Yasuaki Kabe, Takanori Nakane, Ikko Koike, Tatsuya Yamamoto, Yuki Sugiura, Erisa Harada, Kenji Sugase, Tatsuro Shimamura, Mitsuyo Ohmura, Kazumi Muraoka, Ayumi Yamamoto, Takeshi Uchida, So Iwata, Yuki Yamaguchi, Elena Krayukhina, Masanori Noda, Hiroshi Handa, Koichiro Ishimori, Susumu Uchiyama, Takuya Kobayashi, Makoto Suematsu

NATURE COMMUNICATIONS　Vol. 7　2016/03　Research paper (scientific journal)

Novel helical assembly in arginine methyltransferase 8

Sachiko Toma-Fukai, Jun -Dal Kim, Kyung-Eui Parka, Naoyuki Kuwabara, Nobutaka Shimizu, Elena Krayukhina, Susumu Uchiyama, Akiyoshi Fukamizu, Toshiyuki Shimizu

JOURNAL OF MOLECULAR BIOLOGY　Vol. 428　No. 6　p. 1197-1208　2016/03　Research paper (scientific journal)

Mass Spectrometric Characterization of HIV-1 Reverse Transcriptase Interactions with Non-nucleoside Reverse Transcriptase Inhibitors

Ratsupa Thammaporn, Kentaro Ishii, Maho Yagi-Utsumi, Susumu Uchiyama, Supa Hannongbua, Koichi Kato

BIOLOGICAL & PHARMACEUTICAL BULLETIN　Vol. 39　No. 3　p. 450-454　2016/03　Research paper (scientific journal)

Biophysical Characterization of Biopharmaceuticals, Including Antibody Drugs

Susumu Uchiyama

YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN　Vol. 136　No. 3　p. 443-448　2016/03

Small-molecule inhibition of PTPRZ reduces tumor growth in a rat model of glioblastoma

Akihiro Fujikawa, Asako Nagahira, Hajime Sugawara, Kentaro Ishii, Seiichi Imajo, Masahito Matsumoto, Kazuya Kuboyama, Ryoko Suzuki, Naomi Tanga, Masanori Noda, Susumu Uchiyama, Toshiyuki Tomoo, Atsuto Ogata, Makoto Masumura, Masaharu Noda

SCIENTIFIC REPORTS　Vol. 6　2016/02　Research paper (scientific journal)

Suppression of Methionine Oxidation of a Pharmaceutical Antibody Stored in a Polymer-Based Syringe

Amano Masato, Fukui Kiichi, Susumu Uchiyama

JOURNAL OF PHARMACEUTICAL SCIENCES　Vol. 105　No. 2　p. 623-629　2016/02　Research paper (scientific journal)

Suppression of Methionine Oxidation of a Pharmaceutical Antibody Stored in a Polymer-Based Syringe

Amano Masato, Fukui Kiichi, Susumu Uchiyama

JOURNAL OF PHARMACEUTICAL SCIENCES　Vol. 105　No. 2　p. 623-629　2016/02　Research paper (scientific journal)

Experimental design and practical aspects

Fumio Arisaka, Susumu Uchiyama

Analytical Ultracentrifugation: Instrumentation, Software, and Applications　p. 15-21　2016/01/01　Part of collection (book)

Important and essential theoretical aspects of AUC

Susumu Uchiyama, Fumio Arisaka

Analytical Ultracentrifugation: Instrumentation, Software, and Applications　p. 3-14　2016/01/01　Part of collection (book)

Biopharmaceutical evaluation of intermolecular interactions by AUC-SE

Shuntaro Saito, Susumu Uchiyama

Analytical Ultracentrifugation: Instrumentation, Software, and Applications　p. 419-440　2016/01/01　Part of collection (book)

Mass spectrometric analysis of protein-ligand interactions

Ishii Kentaro, Noda Masanori, Uchiyama Susumu

BIOPHYSICS AND PHYSICOBIOLOGY　Vol. 13　p. 87-95　2016

Structural analysis of human chromosome by FIB/SEM

Sasakura Soma, Yoshida Akiyo, Wako Toshiyuki, Kaneyoshi Kohei, Poonperm Rawin, Ogawa Shinichi, Kato Jun, Otsuka Yuji, Takata Hideaki, Uchiyama Susumu, Fukui Kiichi

Chromosome Science　Vol. 19　No. 1-4　p. 25-31　2016

Mass Spectrometry

Noda, Masanori, Fukui, Kiichi, Uchiyama, Susumu

Advanced Methods in Structural Biology　p. 185-198　2016

Disassembly of the self-assembled, double-ring structure of proteasome α7 homo-tetradecamer by α6.

Kentaro Ishii, Masanori Noda, Hirokazu Yagi, Ratsupa Thammaporn, Supaporn Seetaha, Tadashi Satoh, Koichi Kato, Susumu Uchiyama

Scientific reports　Vol. 5　p. 18167-18167　2015/12/14　Research paper (scientific journal)

Chemical mechanism of petal color development of Nemophila menziesii by a metalloanthocyanin, nemophilin

Kumi Yoshida, Kensuke Tojo, Mihoko Mori, Keiko Yamashita, Sayoko Kitahara, Masanori Noda, Susumu Uchiyama

TETRAHEDRON　Vol. 71　No. 48　p. 9123-9130　2015/12　Research paper (scientific journal)

Structural Basis for Dimer Formation of Human Condensin Structural Maintenance of Chromosome Proteins and Its Implications for Single-stranded DNA Recognition

Susumu Uchiyama, Kazuki Kawahara, Yuki Hosokawa, Shunsuke Fukakusa, Hiroya Oki, Shota Nakamura, Yukiko Kojima, Masanori Noda, Rie Takino, Yuya Miyahara, Takahiro Maruno, Yuji Kobayashi, Tadayasu Ohkubo, Kiichi Fukui

JOURNAL OF BIOLOGICAL CHEMISTRY　Vol. 290　No. 49　p. 29461-U428　2015/12　Research paper (scientific journal)

The role of phosphorylation of histone H3 at serine 10 in chromatin condensation in vitro

Equilibrina, Ilma, Krayukhina, Elena, Inoue, Kota, Ishikawa, Yuya, Kawamoto, Akihiro, Kato, Takayuki, Suetake, Isao, Tajima, Shoji, Takata, Hideaki, Uchiyama, Susumu, Fukui, Kiichi

Chromosome Sci.　Vol. 18　No. 1-2　p. 9-14　2015/11

Effects of dehydration and drying steps on human chromosome interior revealed by focused ion beam/scanning electron microscopy (FIB/SEM)

Kaneyoshi, Kohei, Fukuda, Shota, Dwiranti, Astari, Kato, Jun, Otsuka, Yuji, Takata, Hideaki, Uchiyama, Susumu, Ogawa, Shinichi, Fukui, Kiichi

Chromosome Sci.　Vol. 18　No. 1-2　p. 23-28　2015/11　Research paper (scientific journal)

Measurement of Particle Diameter for Nano-particles by Sedimentation Velocity Analytical Ultracentrifugation

Vol. 7　No. 7　p. 626-631　2015/07

Chromosome Scaffold is a Double-Stranded Assembly of Scaffold Proteins

Rawin Poonperm, Hideaki Takata, Tohru Hamano, Atsushi Matsuda, Susumu Uchiyama, Yasushi Hiraoka, Kiichi Fukui

SCIENTIFIC REPORTS　Vol. 5　2015/07　Research paper (scientific journal)

Crystal structure of extracellular domain of human lectin-like transcript 1 (LLT1), the ligand for natural killer receptor-P1A

Shunsuke Kita, Haruki Matsubara, Yoshiyuki Kasai, Takaharu Tamaoki, Yuki Okabe, Hideo Fukuhara, Jun Kamishikiryo, Elena Krayukhina, Susumu Uchiyama, Toyoyuki Ose, Kimiko Kuroki, Katsumi Maenaka

EUROPEAN JOURNAL OF IMMUNOLOGY　Vol. 45　No. 6　p. 1605-1613　2015/06　Research paper (scientific journal)

Development of a novel humanized anti-CD20 chimeric antigen receptor; Effect on the CD20 down-regulated and mAb therapy resistant tumor cells

Keisuke Watanabe, Seitaro Terakura, Susumu Uchiyama, Kazuyuki Shimada, Akihiro Tomita, Tetsuya Nishida, Makoto Murata, Tomoki Naoe, Hitoshi Kiyoi

CLINICAL LYMPHOMA MYELOMA & LEUKEMIA　Vol. 15　p. S217-S217　2015/06

Pepsin immobilization on an aldehyde-modified polymethacrylate monolith and its application for protein analysis

Wenjuan Han, Mika Yamauchi, Urara Hasegawa, Masanori Noda, Kiichi Fukui, Andre J. van der Vlies, Susumu Uchiyama, Hiroshi Uyama

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　Vol. 119　No. 5　p. 505-510　2015/05　Research paper (scientific journal)

A Multilaboratory Comparison of Calibration Accuracy and the Performance of External References in Analytical Ultracentrifugation

Huaying Zhao, Rodolfo Ghirlando, Carlos Alfonso, Fumio Arisaka, Ilan Attali, David L. Bain, Marina M. Bakhtina, Donald F. Becker, Gregory J. Bedwell, Ahmet Bekdemir, Tabot M. D. Besong, Catherine Birck, Chad A. Brautigam, William Brennerman, Olwyn Byron, Agnieszka Bzowska, Jonathan B. Chaires, Catherine T. Chaton, Helmut Coelfen, Keith D. Connaghan, Kimberly A. Crowley, Ute Curth, Tina Daviter, William L. Dean, Ana I. Diez, Christine Ebel, Debra M. Eckert, Leslie E. Eisele, Edward Eisenstein, Patrick England, Carlos Escalante, Jeffrey A. Fagan, Robert Fairman, Ron M. Finn, Wolfgang Fischle, Jose Garcia de la Torre, Jayesh Gor, Henning Gustafsson, Damien Hall, Stephen E. Harding, Jose G. Hernandez Cifre, Andrew B. Herr, Elizabeth E. Howell, Richard S. Isaac, Shu-Chuan Jao, Davis Jose, Soon-Jong Kim, Bashkim Kokona, Jack A. Kornblatt, Dalibor Kosek, Elena Krayukhina, Daniel Krzizike, Eric A. Kusznir, Hyewon Kwon, Adam Larson, Thomas M. Laue, Aline Le Roy, Andrew P. Leech, Hauke Lilie, Karolin Luger, Juan R. Luque-Ortega, Jia Ma, Carrie A. May, Ernest L. Maynard, Anna Modrak-Wojcik, Yee-Foong Mok, Norbert Muecke, Luitgard Nagel-Steger, Geeta J. Narlikar, Masanori Noda, Amanda Nourse, Tomas Obsil, Chad K. Park, Jin-Ku Park, Peter D. Pawelek, Erby E. Perdue, Stephen J. Perkins, Matthew A. Perugini, Craig L. Peterson, Martin G. Peverelli, Grzegorz Piszczek, Gali Prag, Peter E. Prevelige, Bertrand D. E. Raynal, Lenka Rezabkova, Klaus Richter, Alison E. Ringel, Rose Rosenberg, Arthur J. Rowe, Arne C. Rufer, David J. Scott, Javier G. Seravalli, Alexandra S. Solovyova, Renjie Song, David Staunton, Caitlin Stoddard, Katherine Stott, Holger M. Strauss, Werner W. Streicher, John P. Sumida, Sarah G. Swygert, Roman H. Szczepanowski, Ingrid Tessmer, Ronald T. Toth, Ashutosh Tripathy, Susumu Uchiyama, Stephan F. W. Uebel, Satoru Unzai, Anna Vitlin Gruber, Peter H. von Hippel, Christine Wandrey, Szu-Huan Wang, Steven E. Weitzel, Beata Wielgus-Kutrowska, Cynthia Wolberger, Martin Wolff, Edward Wright, Yu-Sung Wu, Jacinta M. Wubben, Peter Schuck

PLOS ONE　Vol. 10　No. 5　2015/05　Research paper (scientific journal)

Structural basis of CpG and inhibitory DNA recognition by Toll-like receptor 9

Umeharu Ohto, Takuma Shibata, Hiromi Tanji, Hanako Ishida, Elena Krayukhina, Susumu Uchiyama, Kensuke Miyake, Toshiyuki Shimizu

NATURE　Vol. 520　No. 7549　p. 702-U303　2015/04　Research paper (scientific journal)

Target Antigen Density Governs the Efficacy of Anti-CD20-CD28-CD3 zeta Chimeric Antigen Receptor-Modified Effector CD8(+) T Cells

Keisuke Watanabe, Seitaro Terakura, Anton C. Martens, Tom van Meerten, Susumu Uchiyama, Misa Imai, Reona Sakemura, Tatsunori Goto, Ryo Hanajiri, Nobuhiko Imahashi, Kazuyuki Shimada, Akihiro Tomita, Hitoshi Kiyoi, Tetsuya Nishida, Tomoki Naoe, Makoto Murata

JOURNAL OF IMMUNOLOGY　Vol. 194　No. 3　p. 911-920　2015/02　Research paper (scientific journal)

Effects of Syringe Material and Silicone Oil Lubrication on the Stability of Pharmaceutical Proteins

Elena Krayukhina, Kouhei Tsumoto, Susumu Uchiyama, Kiichi Fukui

JOURNAL OF PHARMACEUTICAL SCIENCES　Vol. 104　No. 2　p. 527-535　2015/02　Research paper (scientific journal)

Quantitative Laser Diffraction Method for the Assessment of Protein Subvisible Particles

Shinichiro Totoki, Gaku Yamamoto, Kouhei Tsumoto, Susumu Uchiyama, Kiichi Fukui

JOURNAL OF PHARMACEUTICAL SCIENCES　Vol. 104　No. 2　p. 618-626　2015/02　Research paper (scientific journal)

Two Decades of Publishing Excellence in Pharmaceutical Biotechnology

David B. Volkin, Susan Hershenson, Rodney J. Y. Ho, Susumu Uchiyama, Gerhard Winter, John F. Carpenter

JOURNAL OF PHARMACEUTICAL SCIENCES　Vol. 104　No. 2　p. 290-300　2015/02

Structural basis for PPAR gamma transactivation by endocrine-disrupting organotin compounds

Shusaku Harada, Youhei Hiromori, Shota Nakamura, Kazuki Kawahara, Shunsuke Fukakusa, Takahiro Maruno, Masanori Noda, Susumu Uchiyama, Kiichi Fukui, Jun-ichi Nishikawa, Hisamitsu Nagase, Yuji Kobayashi, Takuya Yoshida, Tadayasu Ohkubo, Tsuyoshi Nakanishi

SCIENTIFIC REPORTS　Vol. 5　2015/02　Research paper (scientific journal)

Analysis of Higher Order Structures of Proteins by Mass Spectrometry

UCHIYAMA Susumu, NODA Masanori, ISHII Kentaro

Seibutsu Butsuri　Vol. 55　No. 5　p. 270-273　2015

Condensin in Chromatid Cohesion and Segregation

Susumu Uchiyama, Kiichi Fukui

CYTOGENETIC AND GENOME RESEARCH　Vol. 147　No. 4　p. 212-216　2015

pH-Dependent Assembly and Segregation of the Coiled-Coil Segments of Yeast Putative Cargo Receptors Emp46p and Emp47p.

Kentaro Ishii, Hiroki Enda, Masanori Noda, Megumi Kajino, Akemi Kim, Eiji Kurimoto, Ken Sato, Akihiko Nakano, Yuji Kobayashi, Hirokazu Yagi, Susumu Uchiyama, Koichi Kato

PloS one　Vol. 10　No. 10　2015　Research paper (scientific journal)

Excessively High-Affinity Single-Chain Fragment Variable Region in a Chimeric Antigen Receptor Can Counteract T-Cell Proliferation

Keisuke Watanabe, Seitaro Terakura, Susumu Uchiyama, Anton C. Martens, Tom van Meerten, Hitoshi Kiyoi, Tetsuya Nishida, Tomoki Naoe, Makoto Murata

BLOOD　Vol. 124　No. 21　2014/12

Liquid formulation for antibody drugs

Susumu Uchiyama

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS　Vol. 1844　No. 11　p. 2041-2052　2014/11

Chromosome Interior Observation by Focused Ion Beam/Scanning Electron Microscopy (FIB/SEM) Using Ionic Liquid Technique

Tohru Hamano, Astari Dwiranti, Kohei Kaneyoshi, Shota Fukuda, Reo Kometani, Masayuki Nakao, Hideaki Takata, Susumu Uchiyama, Nobuko Ohmido, Kiichi Fukui

MICROSCOPY AND MICROANALYSIS　Vol. 20　No. 5　p. 1340-1347　2014/10　Research paper (scientific journal)

Detection of Histidine Oxidation in a Monoclonal Immunoglobulin Gamma (IgG) 1 Antibody

Masato Amano, Naoki Kobayashi, Masayuki Yabuta, Susumu Uchiyama, Kiichi Fukui

ANALYTICAL CHEMISTRY　Vol. 86　No. 15　p. 7536-7543　2014/08　Research paper (scientific journal)

The Effect of Magnesium Ions on Chromosome Structure as Observed by Helium Ion Microscopy

Astari Dwiranti, Tohru Hamano, Hideaki Takata, Shoko Nagano, Hongxuan Guo, Keiko Onishi, Toshiyuki Wako, Susumu Uchiyama, Kiichi Fukui

MICROSCOPY AND MICROANALYSIS　Vol. 20　No. 1　p. 184-188　2014/02　Research paper (scientific journal)

2SBP-06 In solution characterizations of protein-protein interactions by analytical ultracentrifugation and mass spectrometry(New Development of Structural Cell Biology in Signal Transduction,Symposium,The 52th Annual Meeting of the Biophysical Society of Japan(BSJ2014))

Uchiyama Susumu

Seibutsu Butsuri　Vol. 54　No. 1　2014

Solution Properties of Antibody Pharmaceuticals

Uchiyama Susumu, Krayukhina Elena, Noda Masanori

Journal of Pharmaceutical Science and Technology, Japan　Vol. 74　No. 1　p. 12-18　2014

SuperNova, a monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation

Kiwamu Takemoto, Tomoki Matsuda, Naoki Sakai, Donald Fu, Masanori Noda, Susumu Uchiyama, Ippei Kotera, Yoshiyuki Arai, Masataka Horiuchi, Kiichi Fukui, Tokiyoshi Ayabe, Fuyuhiko Inagaki, Hiroshi Suzuki, Takeharu Nagai

SCIENTIFIC REPORTS　Vol. 3　2013/09　Research paper (scientific journal)

Thermodynamic Assessment of Domain-Domain Interactions and In Vitro Activities of Mesophilic and Thermophilic Ribosome Recycling Factors

Uchiyama Susumu, Ohshima Atsushi, Yoshida Takuya, Ohkubo Tadayasu, Kobayashi Yuji

BIOPOLYMERS　Vol. 100　No. 4　p. 366-379　2013/07　Research paper (scientific journal)

ASURA (Phb2) interacts with Scc1 through chromatin.

Equilibrina, I, Matsunaga, S, Morimoto, A, Hashimoto, T, Uchiyama, S, Fukui, K

CYTOGENETIC AND GENOME RESEARCH　Vol. 139　No. 4　p. 225-233　2013/06　Research paper (scientific journal)

Visualization of oil body distribution in Jatropha curcas L. by four-wave mixing microscopy

Makiko Ishii, Susumu Uchiyama, Yasuyuki Ozeki, Sin'ichiro Kajiyama, Kazuyoshi Itoh, Kiichi Fukui

Japanese Journal of Applied Physics　Vol. 52　No. 6　p. 62403-62407　2013/06　Research paper (scientific journal)

Characterization of the novel Trypanosoma brucei inosine 5 '-monophosphate dehydrogenase

Tomoaki Bessho, Shoko Morii, Toshihide Kusumoto, Takahiro Shinohara, Masanori Noda, Susumu Uchiyama, Satoshi Shuto, Shigenori Nishimura, Appolinaire Djikeng, Michael Duszenko, Samuel K. Martin, Takashi Inui, Kilunga B. Kubata

PARASITOLOGY　Vol. 140　No. 6　p. 735-745　2013/05　Research paper (scientific journal)

Effects of Ionic Strength and Sugars on the Aggregation Propensity of Monoclonal Antibodies: Influence of Colloidal and Conformational Stabilities

Shuntaro Saito, Jun Hasegawa, Naoki Kobayashi, Toshiaki Tomitsuka, Susumu Uchiyama, Kiichi Fukui

PHARMACEUTICAL RESEARCH　Vol. 30　No. 5　p. 1263-1280　2013/05　Research paper (scientific journal)

Thermodynamic Domain-domain Interactions and in vitro Activities of Mosophilic and Thermophilic Ribosome Recycling Factors

Uchiyama Susumu, Ohshima Atsushi, Yoshida Takuya, Ohkubo Tadayasu, Kobayashi Yuji

BIOPOLYMERS　Vol. 100　No. 3　p. 280-281　2013/05

Aggregation analysis of pharmaceutical human immunoglobulin preparations using size-exclusion chromatography and analytical ultracentrifugation sedimentation velocity

Elena Krayukhina, Susumu Uchiyama, Kiyoko Nojima, Yoshiaki Okada, Isao Hamaguchi, Kiichi Fukui

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　Vol. 115　No. 1　p. 104-110　2013/01　Research paper (scientific journal)

An archaeal homolog of proteasome assembly factor functions as a proteasome activator.

Kentaro Kumoi, Tadashi Satoh, Kazuyoshi Murata, Takeshi Hiromoto, Tsunehiro Mizushima, Yukiko Kamiya, Masanori Noda, Susumu Uchiyama, Hirokazu Yagi, Koichi Kato

PloS one　Vol. 8　No. 3　2013　Research paper (scientific journal)

Protein encapsulation within synthetic molecular hosts

Daishi Fujita, Kosuke Suzuki, Sota Sato, Maho Yagi-Utsumi, Yoshiki Yamaguchi, Nobuhiro Mizuno, Takashi Kumasaka, Masaki Takata, Masanori Noda, Susumu Uchiyama, Koichi Kato, Makoto Fujita

NATURE COMMUNICATIONS　Vol. 3　2012/10　Research paper (scientific journal)

Creation of a Binuclear Purple Copper Site within a de Novo Coiled-Coil Protein

Daigo Shiga, Yasuhiro Funahashi, Hideki Masuda, Akihiro Kikuchi, Masanori Noda, Susumu Uchiyama, Kiichi Fukui, Kenji Kanaori, Kunihiko Tajima, Yu Takano, Haruki Nakamura, Misato Kamei, Toshiki Tanaka

BIOCHEMISTRY　Vol. 51　No. 40　p. 7901-7907　2012/10　Research paper (scientific journal)

Domain 5 of high molecular weight kininogen inhibits collagen-mediated cancer cell adhesion and invasion in association with alpha-actinin-4

Tsunetoshi Hatoh, Toshinaga Maeda, Keisuke Takeuchi, Osamu Ogikubo, Susumu Uchiyama, Takanobu Otsuka, Iwao Ohkubo, Hisakazu Ogita

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　Vol. 427　No. 3　p. 497-502　2012/10　Research paper (scientific journal)

Histones in metaphase chromosomes

Susumu Uchiyama, Kiichi Fukui

Histones: Class, Structure and Function　p. 77-87　2012/09　Part of collection (book)

Effects of rotational speed on the hydrodynamic properties of pharmaceutical antibodies measured by analytical ultracentrifugation sedimentation velocity

Elena Krayukhina, Susumu Uchiyama, Kiichi Fukui

EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES　Vol. 47　No. 2　p. 367-374　2012/09　Research paper (scientific journal)

Chromosome observation by scanning electron microscopy using ionic liquid

Astari Dwiranti, Linyen Lin, Eiko Mochizuki, Susumu Kuwabata, Akio Takaoka, Susumu Uchiyama, Kiichi Fukui

MICROSCOPY RESEARCH AND TECHNIQUE　Vol. 75　No. 8　p. 1113-1118　2012/08　Research paper (scientific journal)

Polymorphism of Collagen Triple Helix Revealed by F-19 NMR of Model Peptide [Pro-4(R)-Hydroxyprolyl-Gly](3)-[Pro-4(R)-Fluoroprolyl-Gly]-[Pro-4(R)-Hydroxyprolyl-Gly](3)

Kazuki Kawahara, Nobuaki Nemoto, Daisuke Motooka, Yoshinori Nishi, Masamitsu Doi, Susumu Uchiyama, Takashi Nakazawa, Yuji Nishiuchi, Takuya Yoshida, Tadayasu Ohkubo, Yuji Kobayashi

JOURNAL OF PHYSICAL CHEMISTRY B　Vol. 116　No. 23　p. 6908-6915　2012/06　Research paper (scientific journal)

A non-canonical UBA-UBL interaction forms the linear-ubiquitin-chain assembly complex

Hirokazu Yagi, Kazuhiro Ishimoto, Takeshi Hiromoto, Hiroaki Fujita, Tsunehiro Mizushima, Yoshinori Uekusa, Maho Yagi-Utsumi, Eiji Kurimoto, Masanori Noda, Susumu Uchiyama, Fuminori Tokunaga, Kazuhiro Iwai, Koichi Kato

EMBO REPORTS　Vol. 13　No. 5　p. 462-468　2012/05　Research paper (scientific journal)

Drug delivery system for poorly water-soluble compounds using lipocalin-type prostaglandin D synthase

Ayano Fukuhara, Hidemitsu Nakajima, Yuya Miyamoto, Katsuaki Inoue, Satoshi Kume, Young-Ho Lee, Masanori Noda, Susumu Uchiyama, Shigeru Shimamoto, Shigenori Nishimura, Tadayasu Ohkubo, Yuji Goto, Tadayoshi Takeuchi, Takashi Inui

JOURNAL OF CONTROLLED RELEASE　Vol. 159　No. 1　p. 143-150　2012/04　Research paper (scientific journal)

RBMX: A Regulator for Maintenance and Centromeric Protection of Sister Chromatid Cohesion

Sachihiro Matsunaga, Hideaki Takata, Akihiro Morimoto, Kayoko Hayashihara, Tsunehito Higashi, Kouhei Akatsuchi, Eri Mizusawa, Mariko Yamakawa, Mamoru Ashida, Tomoko M. Matsunaga, Takachika Azuma, Susumu Uchiyama, Kiichi Fukui

CELL REPORTS　Vol. 1　No. 4　p. 299-308　2012/04　Research paper (scientific journal)

Archaeal ribosomal stalk protein interacts with translation factors in a nucleotide-independent manner via its conserved C terminus

Naoko Nomura, Takayoshi Honda, Kentaro Baba, Takao Naganuma, Takehito Tanzawa, Fumio Arisaka, Masanori Noda, Susumu Uchiyama, Isao Tanaka, Min Yao, Toshio Uchiumi

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　Vol. 109　No. 10　p. 3748-3753　2012/03　Research paper (scientific journal)

Behavior of Monoclonal Antibodies: Relation Between the Second Virial Coefficient (B-2) at Low Concentrations and Aggregation Propensity and Viscosity at High Concentrations

Shuntaro Saito, Jun Hasegawa, Naoki Kobayashi, Naoyuki Kishi, Susumu Uchiyama, Kiichi Fukui

PHARMACEUTICAL RESEARCH　Vol. 29　No. 2　p. 397-410　2012/02　Research paper (scientific journal)

The triple helical structure and stability of collagen model peptide with 4(s)-hydroxyprolyl-pro-gly units

Daisuke Motooka, Kazuki Kawahara, Shota Nakamura, Masamitsu Doi, Yoshinori Nishi, Yuji Nishiuchi, Young Kee Kang, Takashi Nakazawa, Susumu Uchiyama, Takuya Yoshida, Tadayasu Ohkubo, Yuji Kobayashi

BIOPOLYMERS　Vol. 98　No. 2　p. 111-121　2012　Research paper (scientific journal)

Fc domain mediated self-association of an IgG1 monoclonal antibody under a low ionic strength condition

Hirotaka Nishi, Makoto Miyajima, Naoki Wakiyama, Kei Kubota, Jun Hasegawa, Susumu Uchiyama, Kiichi Fukui

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　Vol. 112　No. 4　p. 326-332　2011/10　Research paper (scientific journal)

Assembly states of the nucleosome assembly protein 1 (NAP-1) revealed by sedimentation velocity and non-denaturing MS

Masanori Noda, Susumu Uchiyama, Adam R. McKay, Akihiro Morimoto, Shigeki Misawa, Akihiro Yoshida, Hideto Shimahara, Hiroto Takinowaki, Shota Nakamura, Yuji Kobayashi, Sachihiro Matsunaga, Tadayasu Ohkubo, Carol V. Robinson, Kiichi Fukui

BIOCHEMICAL JOURNAL　Vol. 436　No. 1　p. 101-112　2011/05　Research paper (scientific journal)

Specific Racemization of Heavy-Chain Cysteine-220 in the Hinge Region of Immunoglobulin Gamma 1 as a Possible Cause of Degradation during Storage

Masato Amano, Jun Hasegawa, Naoki Kobayashi, Naoyuki Kishi, Takashi Nakazawa, Susumu Uchiyama, Kiichi Fukui

ANALYTICAL CHEMISTRY　Vol. 83　No. 10　p. 3857-3864　2011/05　Research paper (scientific journal)

The nuclear scaffold protein SAF-A is required for kinetochore-microtubule attachment and contributes to the targeting of Aurora-A to mitotic spindles

Nan Ma, Sachihiro Matsunaga, Akihiro Morimoto, Gyosuke Sakashita, Takeshi Urano, Susumu Uchiyama, Kiichi Fukui

JOURNAL OF CELL SCIENCE　Vol. 124　No. 3　p. 394-404　2011/02　Research paper (scientific journal)

2D1536 Correlation between dimerization ability of 3α-hydroxysteroid dehydrogenase and its enzymatic function(Protein: Structure & Function 1,The 48th Annual Meeting of the Biophysical Society of Japan)

Hara Kotaro, Noda Masanori, Uchiyama Susumu, Ueda Shigeru, Fukui Kiichi, Oda Masayuki

Seibutsu Butsuri　Vol. 51　p. S80-S81　2011

An X-chromosome RNA-binding motif protein (RBMX) is required for proper kinetochore formation

Lee, Mei Hann, Lin, Linyen, Takata, Hideaki, Morimoto, Akihiro, Uchiyama, Susumu, Matsunaga, Sachihiro, Fukui, Kiichi

Chromosome Science　Vol. 14　No. 1-2　p. 17-21　2011　Research paper (scientific journal)

ASURA (PHB2) Is Required for Kinetochore Assembly and Subsequent Chromosome Congression

Mei Hann Lee, Linyen Lin, Ilma Equilibrina, Susumu Uchiyama, Sachihiro Matsunaga, Kiichi Fukui

ACTA HISTOCHEMICA ET CYTOCHEMICA　Vol. 44　No. 6　p. 247-258　2011　Research paper (scientific journal)

Creation of a Type 1 Blue Copper Site within a de Novo Coiled-Coil Protein Scaffold

Daigo Shiga, Daisuke Nakane, Tomohiko Inomata, Yasuhiro Funahashi, Hideki Masuda, Akihiro Kikuchi, Masayuki Oda, Masanori Noda, Susumu Uchiyama, Kiichi Fukui, Kenji Kanaori, Kunihiko Tajima, Yu Takano, Haruki Nakamura, Toshiki Tanake

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY　Vol. 132　No. 51　p. 18191-18198　2010/12　Research paper (scientific journal)

Assembly Modulation by Adjusting Countercharges of Heterobimetallic Supramolecular Polymers Composed of Tris(spiroborate) Twin Bowls

Hiroshi Danjo, Kadzuya Hirata, Masanori Noda, Susumu Uchiyama, Kiichi Fukui, Masatoshi Kawahata, Isao Azumaya, Kentaro Yamaguchi, Toshifumi Miyazawa

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY　Vol. 132　No. 44　p. 15556-15558　2010/11　Research paper (scientific journal)

Structural basis for semaphorin signalling through the plexin receptor

Terukazu Nogi, Norihisa Yasui, Emiko Mihara, Yukiko Matsunaga, Masanori Noda, Naoya Yamashita, Toshihiko Toyofuku, Susumu Uchiyama, Yoshio Goshima, Atsushi Kumanogoh, Junichi Takagi

NATURE　Vol. 467　No. 7319　p. 1123-U138　2010/10　Research paper (scientific journal)

Cloning, expression, crystallization and preliminary X-ray crystallographic analysis of a human condensin SMC2 hinge domain with short coiled coils

Kazuki Kawahara, Shota Nakamura, Yasuhiro Katsu, Daisuke Motooka, Yuki Hosokawa, Yukiko Kojima, Keiko Matsukawa, Hiroto Takinowaki, Susumu Uchiyama, Yuji Kobayashi, Kiichi Fukui, Tadayasu Ohkubo

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS　Vol. 66　No. 9　p. 1067-1070　2010/09　Research paper (scientific journal)

Characterization of HIV-1 resistance to a fusion inhibitor, N36, derived from the gp41 amino-terminal heptad repeat

Kazuki Izumi, Shota Nakamura, Hiroaki Nakano, Kazuya Shimura, Yasuko Sakagami, Shinya Oishi, Susumu Uchiyama, Tadayasu Ohkubo, Yuji Kobayashi, Nobutaka Fujii, Masao Matsuoka, Eiichi N. Kodama

ANTIVIRAL RESEARCH　Vol. 87　No. 2　p. 179-186　2010/08　Research paper (scientific journal)

Phase Separation of an IgG1 Antibody Solution under a Low Ionic Strength Condition

Hirotaka Nishi, Makoto Miyajima, Hiroaki Nakagami, Masanori Noda, Susumu Uchiyama, Kiichi Fukui

PHARMACEUTICAL RESEARCH　Vol. 27　No. 7　p. 1348-1360　2010/07　Research paper (scientific journal)

Thermal effects of added propanol on the helix-coil transition of (Pro-Pro-Gly)(10) in D2O solution: An NMR study

Tsutomu Kai, Susumu Uchiyama, Yoshinori Nishi, Yuji Kobayashi, Tetsuo Tomiyama

CHEMICAL PHYSICS LETTERS　Vol. 491　No. 4-6　p. 208-213　2010/05　Research paper (scientific journal)

Comparative Analysis of Highly Homologous Shewanella Cytochromes c(5) for Stability and Function

Satoshi Takenaka, Satoshi Wakai, Hideyuki Tamegai, Susumu Uchiyama, Yoshihiro Sambongi

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY　Vol. 74　No. 5　p. 1079-1083　2010/05　Research paper (scientific journal)

Structural basis for the cooperative interplay between the two causative gene products of combined factor V and factor VIII deficiency

Miho Nishio, Yukiko Kamiya, Tsunehiro Mizushima, Soichi Wakatsuki, Hiroaki Sasakawa, Kazuo Yamamoto, Susumu Uchiyama, Masanori Noda, Adam R. McKay, Kiichi Fukui, Hans-Peter Hauri, Koichi Kato

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　Vol. 107　No. 9　p. 4034-4039　2010/03　Research paper (scientific journal)

The Middle Region of an HP1-binding Protein, HP1-BP74, Associates with Linker DNA at the Entry/Exit Site of Nucleosomal DNA

Kayoko Hayashihara, Susumu Uchiyama, Shigeru Shimamoto, Shouhei Kobayashi, Miroslav Tomschik, Hidekazu Wakamatsu, Daisuke No, Hiroki Sugahara, Naoto Hori, Masanori Noda, Tadayasu Ohkubo, Jordanka Zlatanova, Sachihiro Matsunaga, Kiichi Fukui

JOURNAL OF BIOLOGICAL CHEMISTRY　Vol. 285　No. 9　p. 6498-6507　2010/02　Research paper (scientific journal)

Structure and Folding of Cytochromes c with Different Thermal Stability

SAMBONGI Yoshihiro, YAMANAKA Masaru, HASEGAWA Jun, UCHIYAMA Susumu

Calorimetry and thermal analysis.　Vol. 37　No. 1　p. 9-16　2010/01/31

1P035 Roles of Arg251 and Phe255 at the dimer interface of 3α-hydroxysteroid dehydrogenase(Protein:Structure & Function,The 48th Annual Meeting of the Biophysical Society of Japan)

Hara Kotaro, Takagi Yuichiro, Noda Masanori, Uchiyama Susumu, Ueda Shigeru, Fukui Kiichi, Oda Masayuki

Seibutsu Butsuri　Vol. 50　No. 2　2010

Development of novel humanized anti-CD20 antibodies based on affinity constant and epitope

Susumu Uchiyama, Yasuhiko Suzuki, Kentaro Otake, Masami Yokoyama, Mitsuo Ohta, Shuichi Aikawa, Midori Komatsu, Tetsuji Sawada, Yoshitoyo Kagami, Yasuo Morishima, Kiichi Fukui

CANCER SCIENCE　Vol. 101　No. 1　p. 201-209　2010/01　Research paper (scientific journal)

Effects of antibody affinity and antigen valence on molecular forms of immune complexes

Masayuki Oda, Susumu Uchiyama, Masanori Noda, Yoshinori Nishi, Maiko Koga, Kouta Mayanagi, Carol V. Robinson, Kiichi Fukui, Yuji Kobayashi, Kosuke Morikawa, Takachika Azuma

MOLECULAR IMMUNOLOGY　Vol. 47　No. 2-3　p. 357-364　2009/12　Research paper (scientific journal)

Regulation of microtubules by a nuclear scaffold attachment factor

Sachihiro Matsunaga, Nan Ma, Akihiro Morimoto, Susumu Uchiyama, Kiichi Fukui

GENES & GENETIC SYSTEMS　Vol. 84　No. 6　p. 487-487　2009/12

The Effect of the Side Chain Length of Asp and Glu on Coordination Structure of Cu2+ in a de novo Designed Protein

Daigo Shiga, Daisuke Nakane, Tomohiko Inomata, Hideki Masuda, Masayuki Oda, Masanori Noda, Susumu Uchiyama, Kiichi Fukui, Yu Takano, Haruki Nakamura, Toshihisa Mizuno, Toshiki Tanaka

BIOPOLYMERS　Vol. 91　No. 11　p. 907-916　2009/11　Research paper (scientific journal)

H2A.Z and H3.3 Histone Variants Affect Nucleosome Structure: Biochemical and Biophysical Studies

Amit Thakar, Pooja Gupta, Toyotaka Ishibashi, Ron Finn, Begonia Silva-Moreno, Susumu Uchiyama, Kiichi Fukui, Miroslav Tomschik, Juan Ausio, Jordanka Zlatanova

BIOCHEMISTRY　Vol. 48　No. 46　p. 10852-10857　2009/11　Research paper (scientific journal)

Hyperstability and crystal structure of cytochrome c(555) from hyperthermophilic Aquifex aeolicus

Marii Obuchi, Kazuki Kawahara, Daisuke Motooka, Shota Nakamura, Masaru Yamanaka, Taku Takeda, Susumu Uchiyama, Yuji Kobayashi, Tadayasu Ohkubo, Yoshihiro Sambongi

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY　Vol. 65　No. 8　p. 804-813　2009/08　Research paper (scientific journal)

A nucleolar protein RRS1 contributes to chromosome congression

Gambe, Arni E., Matsunaga, Sachihiro, Takata, Hideaki, Ono-Maniwa, Rika, Baba, Akiko, Uchiyama, Susumu, Fukui, Kiichi

Febs Letters　Vol. 583　No. 12　p. 1951-1956　2009/06/18　Research paper (scientific journal)

A nucleolar protein RRS1 contributes to chromosome congression

Arni E. Gambe, Sachihiro Matsunaga, Hideaki Takata, Rika Ono-Maniwa, Akiko Baba, Susumu Uchiyama, Kiichi Fukui

FEBS LETTERS　Vol. 583　No. 12　p. 1951-1956　2009/06　Research paper (scientific journal)

Bioactive beads-mediated transformation of rice with large DNA fragments containing Aegilops tauschii genes

Naoki Wada, Shin'ichiro Kajiyama, Yukio Akiyama, Shigeki Kawakami, Daisuke No, Susumu Uchiyama, Motoyasu Otani, Takiko Shimada, Naoko Nose, Go Suzuki, Yasuhiko Mukai, Kiichi Fukui

PLANT CELL REPORTS　Vol. 28　No. 5　p. 759-768　2009/05　Research paper (scientific journal)

Electrical Detection of Extended λDNA Molecules with Gap Electrodes by Ultraviolet Radiation

Takamichi Hanada, Yoshifumi Okamoto, Katsuhiro Hashimoto, Yasuhumi Ochi, Takaaki Hirokane, Shinichiro Kajiyama, Susumu Uchiyama, Kiichi Fukui, Kenta Arima, Junichi Uchikoshi, Mizuho Morita

Extended Abstracts of First International Symposium on Atomically Controlled Fabrication Technology - Surface and Thin Film Processing -　2009/02

The proteome of human metaphase chromosomes

Uchiyama, Susumu

Chromosome Science　Vol. 12　No. 1-2　2009　Research paper (scientific journal)

2DE for proteome analysis of human metaphase chromosomes.

Kiichi Fukui, Susumu Uchiyama

Methods in molecular biology (Clifton, N.J.)　Vol. 519　p. 259-271　2009　Research paper (scientific journal)

Stability enhancement of cytochrome c through heme deprotonation and mutations

Takafumi Sonoyama, Jun Hasegawa, Susumu Uchiyama, Shota Nakamura, Yuji Kobayashi, Yoshihiro Sambongi

BIOPHYSICAL CHEMISTRY　Vol. 139　No. 1　p. 37-41　2009/01　Research paper (scientific journal)

Epigenetic regulation of plant development by a histone methyltransferase SDG4

Sachihiro Matsunaga, Joyce Cartagena, Motoaki Seki, Daisuke Kurihara, Kazuo Shinozaki, Susumu Uchiyama, Kiichi Fukui

GENES & GENETIC SYSTEMS　Vol. 83　No. 6　p. 493-493　2008/12

Nucleophosmin is required for chromosome congression, proper mitotic spindle formation, and kinetochore-microtubule attachment in HeLa cells

Amin, Mohammed Abdullahel a, Matsunaga, Sachihiro, Uchiyama, Susumu, Fukui, Kiichi

Febs Letters　Vol. 582　No. 27　p. 3839-3844　2008/11/12　Research paper (scientific journal)

Nucleophosmin is required for chromosome congression, proper mitotic spindle formation, and kinetochore-microtubule attachment in HeLa cells

Mohammed Abdullahel Amin, Sachihiro Matsunaga, Susumu Uchiyama, Kiichi Fukui

FEBS LETTERS　Vol. 582　No. 27　p. 3839-3844　2008/11　Research paper (scientific journal)

Depletion of nucleophosmin leads to distortion of nucleolar and nuclear structures in HeLa cells

Mohammed Abdullahel Amin, Sachihiro Matsunaga, Susumu Uchiyama, Kiichi Fukui

BIOCHEMICAL JOURNAL　Vol. 415　No. 3　p. 345-351　2008/11　Research paper (scientific journal)

Isolation method for human metaphase chromosomes.

Hayashihara, K, Uchiyama, S, Kobayashi, S, Yanagisawa, M, Matsunaga, S, Fukui, K

Protocol Exchange　2008/08　Research paper (scientific journal)

Live cell imaging reveals plant Aurora kinase has dual roles during mitosis

Daisuke Kurihara, Sachihiro Matsunaga, Susumu Uchiyama, Kiichi Fukui

PLANT AND CELL PHYSIOLOGY　Vol. 49　No. 8　p. 1256-1261　2008/08　Research paper (scientific journal)

Synthesis and characterization of the collagen model peptides containing 4(S)-hydroxyproline

Daisuke Motooka, Kazuki Kawahara, Shota Nakamura, Susumu Uchiyama, Masamitsu Doi, Yuji Nishiuchi, Takashi Nakazawa, Tadayasu Ohkubo, Yoshinori Nishi, Yuji Kobayashi

JOURNAL OF PEPTIDE SCIENCE　Vol. 14　No. 8　p. 172-172　2008/08

F-19-NMR study of collagen model peptides containing 4(R)-fluoroproline

Kazuki Kawahara, Nobuaki Nemoto, Daisuke Motooka, Susumu Uchiyama, Masamitsu Doi, Yuji Nishiuchi, Takashi Nakazawa, Takuya Yoshida, Tadayasu Ohkubo, Yoshinori Nishi, Yuji Kobayashi

JOURNAL OF PEPTIDE SCIENCE　Vol. 14　No. 8　p. 175-175　2008/08

Stabilization mechanism of cytochrome c(552) from a moderately thermophilic bacterium, Hydrogenophilus thermoluteolus

Sayaka Hakamada, Takafumi Sonoyama, Shin-ichi Ichiki, Shota Nakamura, Susumu Uchiyama, Yuji Kobayashi, Yoshihiro Sambongi

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY　Vol. 72　No. 8　p. 2103-2109　2008/08　Research paper (scientific journal)

The Arabidopsis SDG4 contributes to the regulation of pollen tube growth by methylation of histone H3 lysines 4 and 36 in mature pollen

Joyce A. Cartagena, Sachihiro Matsunaga, Motoaki Seki, Daisuke Kurihara, Masami Yokoyama, Kazuo Shinozaki, Satoru Fujimoto, Yoshitaka Azumi, Susumu Uchiyama, Kiich Fukui

DEVELOPMENTAL BIOLOGY　Vol. 315　No. 2　p. 355-368　2008/03　Research paper (scientific journal)

Anti-peptide antibodies for examining the conformation, molecular assembly and localization of an intracellular protein, ribosomal protein S6, in vivo

Masatoshi Nakagawa, Nobuko Ohmid, Katsumi Ishikawa, Susumu Uchiyama, Kiichi Fukui, Takachika Azuma

JOURNAL OF BIOCHEMISTRY　Vol. 143　No. 3　p. 325-332　2008/03　Research paper (scientific journal)

Preparation methods of human metaphase chromosomes for their proteome analysis

Kiichi Fukui, Hideaki Takata, Susumu Uchiyama

Methods in Molecular Biology　Vol. 432　p. 149-160　2008　Research paper (scientific journal)

Plant Aurora kinase has dual roles on chromosome alignment and segregation during mitosis.

Kurihara D, Matsunaga S, Uchiyama S, Fukui K

CYTOLOGIA　Vol. 73　p. 1-2　2008

Thermal unfolding mechanism of lipocalin-type prostaglandin D synthase

Tsukimi Iida, Shigenori Nishimura, Maki Mochizuki, Susumu Uchiyama, Tadayasu Ohkubo, Yoshihiro Urade, Akiyoshi Tanaka, Takashi Inui

FEBS JOURNAL　Vol. 275　No. 2　p. 233-241　2008/01　Research paper (scientific journal)

Calcite-specific coupling protein in barnacle underwater cement

Youichi Mori, Youhei Urushida, Masahiro Nakano, Susumu Uchiyama, Kei Kamino

FEBS JOURNAL　Vol. 274　No. 24　p. 6436-6446　2007/12　Research paper (scientific journal)

Aurora kinase is required for chromosome segregation in plants

Daisuke Kurihara, Masanori Noda, Akira Kawabe, Satoru Fujimoto, Susumu Uchiyama, Sachihiro Matsunaga, Kiichi Fukui

GENES & GENETIC SYSTEMS　Vol. 82　No. 6　p. 560-560　2007/12

A novel cascade to regulate chromosome cohesion during mitosis

Sachihiro Matsunaga, Hideaki Takata, Akihiro Morimoto, Nan Ma, Daisuke Kurihara, Rika Ono-Maniwa, Susumu Uchiyama, Kiichi Fukui

GENES & GENETIC SYSTEMS　Vol. 82　No. 6　p. 560-560　2007/12

Roles of a short connecting disulfide bond in the stability and function of psychrophilic Shewanella violacea cytochrome c(5)*

Keiko Ogawa, Takafumi Sonoyama, Taku Takeda, Shin-ichi Ichiki, Shota Nakamura, Yuji Kobayashi, Susumu Uchiyama, Kaoru Nakasone, Shin-ichi J. Takayama, Hajime Mita, Yasuhiko Yamamoto, Yoshihiro Sambongi

EXTREMOPHILES　Vol. 11　No. 6　p. 797-807　2007/11　Research paper (scientific journal)

Whole-mount immunoelectron tomography of chromosomes and cells.

Engelhardt, P, Merillinen, J, Zhao, F, Uchiyama, S, Fukui, K, Lehto, VP

Methods Mol Biol.　Vol. 369　p. 387-405　2007/09　Research paper (scientific journal)

Fibrillarin, a nucleolar protein, is required for normal nuclear morphology and cellular growth in HeLa cells

Mohammed Abdullahel Amin, Sachihiro Matsunaga, Nan Ma, Hideaki Takata, Masami Yokoyama, Susumu Uchiyama, Kiichi Fukui

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　Vol. 360　No. 2　p. 320-326　2007/08　Research paper (scientific journal)

H1.X with different properties from other linker histories is required for mitotic progression

Hideaki Takata, Sachihiro Matsunaga, Akihiro Morimoto, Rika Ono-Maniwa, Susumu Uchiyama, Kiichi Fukui

FEBS LETTERS　Vol. 581　No. 20　p. 3783-3788　2007/08　Research paper (scientific journal)

PHB2 protects sister-chromatid cohesion in mitosis

Hideaki Takata, Sachihiro Matsunaga, Akihiro Morimoto, Nan Ma, Daisuke Kurihara, Rika Ono-Maniwa, Masatoshi Nakagawa, Takachika Azuma, Susumu Uchiyama, Kiichi Fukui

CURRENT BIOLOGY　Vol. 17　No. 15　p. 1356-1361　2007/08　Research paper (scientific journal)

Histone H2A mobility is regulated by its tails and acetylation of core histone tails

Tsunehito Higashi, Sachihiro Matsunaga, Keisuke Isobe, Akihiro Morimoto, Tomoko Shimada, Shogo Kataoka, Wataru Watanabe, Susumu Uchiyama, Kazuyoshi Itoh, Kiichi Fukui

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　Vol. 357　No. 3　p. 627-632　2007/06　Research paper (scientific journal)

Nucleolin functions in nucleolus formation and chromosome congression

Nan Ma, Sachihiro Matsunaga, Hideaki Takata, Rika Ono-Maniwa, Susumu Uchiyama, Kiichi Fukui

JOURNAL OF CELL SCIENCE　Vol. 120　No. 12　p. 2091-2105　2007/06　Research paper (scientific journal)

Crystal structure of Pyrococcus horikoshii PPC protein at 1.60 a resolution

Linyen Lin, Hiroaki Nakano, Shota Nakamura, Susumu Uchiyama, Satoru Fujimoto, Sachihiro Matsunaga, Yuji Kobayashi, Tadayasu Ohkubo, Kiichi Fukui

PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS　Vol. 67　No. 2　p. 505-507　2007/05　Research paper (scientific journal)

Development of a multistage classifier for a monitoring system of cell activity based on imaging of chromosomal dynamics

Arni E. Gambe, Rika Maniwa Ono, Sachihiro Matsunaga, Natsumaro Kutsuna, Takumi Higaki, Tsunehito Higashi, Seiichiro Hasezawa, Susumu Uchiyama, Kiichi Fukui

CYTOMETRY PART A　Vol. 71A　No. 5　p. 286-296　2007/05　Research paper (scientific journal)

A comparative proteome analysis of human metaphase chromosomes isolated from two different cell lines reveals a set of conserved chromosome-associated proteins

Hideaki Takata, Susumu Uchiyama, Naoko Nakamura, Shoko Nakashima, Shouhei Kobayashi, Takefumi Sone, Sumiko Kimura, Sunshine Lahmers, Henk Granzier, Siegfried Labeit, Sachihiro Matsunaga, Kiichi Fukui

GENES TO CELLS　Vol. 12　No. 3　p. 269-284　2007/03　Research paper (scientific journal)

Characterization of a splicing variant of plant Aurora kinase

Daisuke Kurihara, Akira Kawabe, Sachihiro Matsunaga, Katsuyuki Nakagawa, Satoru Fujimoto, Susumu Uchiyama, Kiichi Fukui

PLANT AND CELL PHYSIOLOGY　Vol. 48　No. 2　p. 369-374　2007/02　Research paper (scientific journal)

The chromosome peripheral protein nucleolin locates in the chromosome periphery and functions in chromosome congress.

Ma, N, Matsunaga, S, Takata, H, Uchiyama, S, Fukui, K

CYTOLOGIA　No. 72　p. 1-2　2007

Chromosome protein framework from proteome analysis of isoated human metaphase chromosomes

Kiichi Fukui, Susumu Uchiyama

CHEMICAL RECORD　Vol. 7　No. 4　p. 230-237　2007　Research paper (scientific journal)

Dynamic analyses of plant nuclei and chromosome-specific proteins

Sachihiro Matsunaga, Daisuke Kurihara, Satoru Fujimoto, Susumu Uchiyama, Kiichi Fukui

PLANT AND CELL PHYSIOLOGY　Vol. 48　p. S7-S7　2007

Proteome analyses of human metaphase chromosomes

Susumu Uchiyama, Shouhei Kobayashi, Hideaki Takata, Sachihiro Matsunaga, Fukui Kiichi

CHROMOSOME RESEARCH　Vol. 15　p. 15-15　2007

Morphological changes of human chromosomes depending on the concentration of divalentcations

Linyen Lin, Susumu Uchiyama, Satoshi Fujimoto, Masanobu Yanagisawa, Akio Takaoka, Sumire Inaga, Sachihiro Matsunaga, Kiichi Fukui

CHROMOSOME RESEARCH　Vol. 15　p. 18-18　2007

Development of a chromosome dynamics imaging-based classifier for monitoring cell activity

Arni Gambe, Rika Ono, Sachihiro Matsunaga, Natsumaro Kutsuna, Takumi Higaki, Tsunehito Higashi, Seiichiro Hasezawa, Susumu Uchiyama, Kiichi Fukui

CHROMOSOME RESEARCH　Vol. 15　p. 56-57　2007

Aurora kinases is required for chromosome segregation in tobacco BY-2 cells

Daisuke Kurihara, Masanori Noda, Akira Kawabe, Satoru Fujimoto, Susumu Uchiyama, Sachihiro Matsunaga, Kiichi Fukui

CHROMOSOME RESEARCH　Vol. 15　p. 97-98　2007

A nucleolar protein, nucleolin, functions in chromosome congression

Sachihiro Matsunaga, Nan Ma, Hideaki Takata, Susumu Uchiyama, Kiichi Fukui

GENES & GENETIC SYSTEMS　Vol. 81　No. 6　p. 431-431　2006/12

Functional analysis of chromosome related proteins with special reference to RRS1

Kiichi Fukui, Sachihiro Matsunaga, Susumu Uchiyama, Rika Maniwa, Akiko Baba

GENES & GENETIC SYSTEMS　Vol. 81　No. 6　p. 432-432　2006/12

Aurora kinase is required for chromosome segregation in tobacco BY-2 cells

Daisuke Kurihara, Sachihiro Matsunaga, Akira Kawabe, Satoru Fujimoto, Masanori Noda, Susumu Uchiyama, Kiichi Fukui

PLANT JOURNAL　Vol. 48　No. 4　p. 572-580　2006/11　Research paper (scientific journal)

Apo- and holo-structures of 3 alpha-hydroxysteroid dehydrogenase from Pseudomonas sp B-0831 - Loop-helix transition induced by coenzyme binding

Shota Nakamura, Masayuki Oda, Sachiyo Kataoka, Shigeru Ueda, Susumu Uchiyama, Takuya Yoshida, Yuji Kobayashi, Tadayasu Ohkubo

JOURNAL OF BIOLOGICAL CHEMISTRY　Vol. 281　No. 42　p. 31876-31884　2006/10　Research paper (scientific journal)

Crystallization and preliminary X-ray analysis of the complex of NADH and 3 alpha-hydroxysteroid dehydrogenase from Pseudomonas sp B-0831

S Kataoka, S Nakamura, T Ohkubo, S Ueda, S Uchiyama, Y Kobayashi, M Oda

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS　Vol. 62　No. 6　p. 569-571　2006/06　Research paper (scientific journal)

Structure of cytochrome c552 from a moderate thermophilic bacterium, Hydrogenophilus thermoluteolus: Comparative study on the thermostability of cytochrome c

S Nakamura, S Ichiki, H Takashima, S Uchiyama, J Hasegawa, Y Kobayashi, Y Sambongi, T Ohkubo

BIOCHEMISTRY　Vol. 45　No. 19　p. 6115-6123　2006/05　Research paper (scientific journal)

Regional and segmental flexibility of antibodies in interaction with antigens of different size

M Oda, S Uchiyama, CV Robinson, K Fukui, Y Kobayashi, T Azuma

FEBS JOURNAL　Vol. 273　No. 7　p. 1476-1487　2006/04　Research paper (scientific journal)

Effect of hydration on the thermal stability of the collagen model peptide containing 4(S)-hydroxyproline

Yoshinri Nishi, other people

Peptide Science 2006　Vol. 2006　p. 169-170　2006　Research paper (scientific journal)

Calreticulin as a new histone binding protein in mitotic chromosomes

S. Kobayashi, S. Uchiyama, T. Sone, M. Noda, L. Lin, H. Mizuno, S. Matsunaga, K. Fukui

CYTOGENETIC AND GENOME RESEARCH　Vol. 115　No. 1　p. 10-15　2006　Research paper (scientific journal)

Effect of hydration on the stability of the collagen-like triple-helical structure of [4(R)-hydroxyprolyl-4(R)-hydroxyprolylglycine](10)

K Kawahara, Y Nishi, S Nakamura, S Uchiyama, Y Nishiuchi, T Nakazawa, T Ohkubo, Y Kobayashi

BIOCHEMISTRY　Vol. 44　No. 48　p. 15812-15822　2005/12　Research paper (scientific journal)

Generation of monoclonal antibodies against chromosomal antigens that have a high sequence similarity between human and mouse

T Higashi, S Miyakawa, S Uchiyama, S Matsunaga, H Takata, S Fujimoto, M Noda, A Terauchi, T Shimizu, M Oda, T Azuma, K Fukui

JOURNAL OF BIOTECHNOLOGY　Vol. 120　No. 3　p. 262-272　2005/11　Research paper (scientific journal)

Collagen-like triple helix formation of synthetic (Pro-Pro-Gly)10 analogues: (4(S)-hydroxyprolyl-4(R)-hydroxyprolyl-Gly)10, (4(R)-hydroxyprolyl-4(R)-hydroxyprolyl-Gly)10 and (4(S)-fluoroprolyl-4(R)-fluoroprolyl-Gly)10.

Masamitsu Doi, Yoshinori Nishi, Susumu Uchiyama, Yuji Nishiuchi, Hideki Nishio, Takashi Nakazawa, Tadayasu Ohkubo, Yuji Kobayashi

Journal of peptide science : an official publication of the European Peptide Society　Vol. 11　No. 10　p. 609-16　2005/10　Research paper (scientific journal)

Collagen-like triple helix formation of synthetic (Pro-Pro-Gly)(10) analogues: (4(S)-hydroxyprolyl-4(R)-hydroxyprolyi-Gly)(10), (4(R)-hydroxyprolyl-4(R)-hydroxyprolyl-Gly)(10) and (4(S)-fluoroprolyl-4(R)-fluoroprolyl-Gly)(10)

M Doi, Y Nishi, S Uchiyama, Y Nishiuchi, H Nishio, T Nakazawa, T Ohkubo, Y Kobayashi

JOURNAL OF PEPTIDE SCIENCE　Vol. 11　No. 10　p. 609-616　2005/10　Research paper (scientific journal)

Characterization and dynamic analysis of Arabidopsis condensin subunits, AtCAP-H and AtCAP-H2

S Fujimoto, M Yonemura, S Matsunaga, T Nakagawa, S Uchiyama, K Fukui

PLANTA　Vol. 222　No. 2　p. 293-300　2005/10　Research paper (scientific journal)

Fluorescent labeling of plant chromosomes in suspension by FISH

Youzhi Ma, Jai Heon Lee, Lian Cheng Li, Susumu Uchiyama, Nobuko Ohmido, Kiichi Fukui

Genes and Genetic Systems　Vol. 80　No. 1　p. 35-39　2005/06　Research paper (scientific journal)

Dynamic analysis of EGFP-histone h1 by two-photon FRAP

Tsunehito Higashi, Keisuke Isobe, Wataru Watanabe, Susumu Uchiyama, Sachihir Matsunaga, Kazuyoshi Itoh, Kiichi Fukui

CELL STRUCTURE AND FUNCTION　Vol. 30　p. 65-65　2005/06

Characterization and localization analysis of Arabidopsis condensin subunits, AtCAP-H and AtCAP-H2

Satoru Fujimoto, Masataka Yonemura, Sachihiro Matsunaga, Susumu Uchiyama, Kiichi Fukui

CELL STRUCTURE AND FUNCTION　Vol. 30　p. 93-93　2005/06

Localization analyses of human metaphase chromosome proteins

Kayoko Hayashihara, Shouhei Kobayashi, Susumu Uchiyama, Sachihiro Matsunaga, Nobuko Ohmindo, Kiichi Fukui

CELL STRUCTURE AND FUNCTION　Vol. 30　p. 93-93　2005/06

Identification and characterization of plant Aurora kinases

Sachihiro Matsunaga, Daisule Kurihara, Akira Kawabe, Susumu Uchiyama, Kiichi Fukui

CELL STRUCTURE AND FUNCTION　Vol. 30　p. 92-92　2005/06

「ナノメディクスを利用した新規医薬品の開発」

福井希一, 内山進

大阪大学産業科学研究所 産業科学ナノテクノロジーセンター オープンラボラトリー 研究成果報告書　2005/05　Research paper (bulletin of university, research institution)

Characterization of plant Aurora kinases during mitosis

A Kawabe, S Matsunaga, K Nakagawa, D Kurihara, A Yoneda, S Hasezawa, S Uchiyama, K Fukui

PLANT MOLECULAR BIOLOGY　Vol. 58　No. 1　p. 1-13　2005/05　Research paper (scientific journal)

Proteome analysis of human metaphase chromosomes.

Uchiyama, S, Kobayashi, S, Takata, H, Ishihara, T, Hori, N, Higashi, T, Hayashihara, K, Sone, T, Higo, D, Nirasawa, T, Takao, T, Matsunaga, S, Fukui, K

J. Biol. Chem.　Vol. 12　No. 4　p. 269-284　2005/04　Research paper (scientific journal)

Different effects of 4-hydroxyproline and 4-fluoroproline on the stability of collagen triple helix

Y Nishi, S Uchiyama, M Doi, Y Nishiuchi, T Nakazawa, T Ohkubo, Y Kobayashi

BIOCHEMISTRY　Vol. 44　No. 16　p. 6034-6042　2005/04　Research paper (scientific journal)

Cloning, expression, crystallization and preliminary X-ray characterization of cytochrome c(552) from a moderate thermophilic bacterium, Hydrogenophilus thermoluteolus

S Ichiki, S Nakamura, T Ohkubo, Y Kobayashi, J Hasegawa, S Uchiyama, H Nishihara, K Mizuta, Y Sambongi

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS　Vol. 61　No. 4　p. 395-398　2005/04　Research paper (scientific journal)

Crystallization and preliminary X-ray crystallographic analysis of a conserved domain in plants and prokaryotes from Pyrococcus horikoshii OT3

LY Lin, H Nakano, S Uchiyama, S Fujimoto, S Matsunaga, S Nakamura, Y Kobayashi, T Ohkubo, K Fukui

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS　Vol. 61　No. 4　p. 414-416　2005/04　Research paper (scientific journal)

Proteome analysis of human metaphase chromosomes

S Uchiyama, S Kobayashi, H Takata, T Ishihara, N Hori, T Higashi, K Hayashihara, T Sone, D Higo, T Nirasawa, T Takao, S Matsunaga, K Fukui

JOURNAL OF BIOLOGICAL CHEMISTRY　Vol. 280　No. 17　p. 16994-17004　2005/04　Research paper (scientific journal)

Five amino acid residues responsible for the high stability of Hydrogenobacter thermophilus cytochrome c(552) - Reciprocal mutation analysis

K Oikawa, S Nakamura, T Sonoyama, A Ohshima, Y Kobayashi, SJ Takayama, Y Yamamoto, S Uchiyama, J Hasegawa, Y Sambongi

JOURNAL OF BIOLOGICAL CHEMISTRY　Vol. 280　No. 7　p. 5527-5532　2005/02　Research paper (scientific journal)

Development of a novel monitoring system for cell activity based on visualization of chromosome dynamics

Gambe, Arni, Maniwa, Rika, Kutsuna, Natsumaro, Higaki, Takumi, Hasezawa, Seiichiro, Uchiyama, Susumo, Matsunaga, Sachihiro, Fukui, Kiichi

Cell Structure and Function　Vol. 30　p. 117-117　2005　Research paper (scientific journal)

2P084 Crystallographic Analysis of Collagen-Like Triple Helical Structure of (4(R)-Hyp-4(R)-Hyp-Gly)_<10>

Kawahara K., Nishi Y., Nakamura S., Uchiyama S., Nishiuchi Y., Nakazawa T., Ohkubo T., Kobayashi Y.

Seibutsu Butsuri　Vol. 45　2005

1P066 Hydration effects on the thermal stability of collagen model peptide, (4(R)-hydroxyprolyl-4(R)-hydroxyprolyl-Gly)_<10>

Nishi Y., Kawahara K., Nakamura S., Uchiyama S., Nishiuchi Y., Nakazawa T., Ohkubo T., Kobayashi Y.

Seibutsu Butsuri　Vol. 45　2005

Changes in chromosomal surface structure are directly related to protein composition.

Fukui, K, Uchiyama, S, Sone, T, Iwano, M, Matsunaga, S

Chromosome Today　2005/01　Research paper (other academic)

Dynamic analyses of Aurora kinases in plants

S Matsunaga, A Kawabe, K Nakagawa, D Kurihara, A Yoneda, S Hasezawa, S Uchiyama, K Fukui

PLANT AND CELL PHYSIOLOGY　Vol. 46　p. S66-S66　2005　Research paper (other academic)

Stabilization mechanism of collagen-like triple helical structure of [4(R)-hydroxyprolyl-4(R)-hydroxyprolyl-glycine]₁₀

NAKAZAWA Takashi, Yoshinori Nishi, thers

Peptide Science 2005　Vol. 2005　p. 393-396　2005　Research paper (scientific journal)

Dynamics of the plant Aurora kinase AtAUR3 during mitosis and spindle abnormality induced by AtAUR3 overexpression.

Matsunaga, S, Kurihara, D, Kawabe, A, Nakagawa, K, Yoneda, A, Hasezawa, S, Uchiyama, S, Fukui, K

CYTOLOGIA　Vol. 70　No. 4　p. 1-2　2005　Research paper (scientific journal)

Complete thermal-unfolding profiles of oxidized and reduced cytochromes c

Susumu Uchiyama, Atsushi Ohshima, Shota Nakamura, Jun Hasegawa, Norifumi Terui, Shin-Ichi Joseph Takayama, Yasuhiko Yamamoto, Yoshihiro Sambongi, Yuji Kobayashi

Journal of the American Chemical Society　Vol. 126　No. 45　p. 14684-14685　2004/11/17　Research paper (scientific journal)

Synthesis of long Poly(dG)·Poly(dC) DNA using enzymatic reaction

Shin-Ichi Tanaka, Masateru Taniguchi, Susumu Uchiyama, Kiichi Fukui, Tomoji Kawai

Chemical Communications　Vol. 10　No. 21　p. 2388-2389　2004/11/07　Research paper (scientific journal)

Development of new dosimetry using extended DNA fibers

S Matsunaga, K Ohshio, E Harada, S Fujiwara, S Uchiyama, K Fukui

JOURNAL OF BIOSCIENCE AND BIOENGINEERING　Vol. 98　No. 5　p. 384-386　2004/11　Research paper (scientific journal)

3P011 Expression of thermophilc hydrogen-oxidizing bacterium Hydrogenobactor thermophilus cytochrome c552 in Escherichia coli and its stability

Oikawa,K, Nakamura,S, Takayama,S, Hasegawa,J, Kobayashi,Y, Yamamoto,Y, Uchiyama,S, Sambongi,Y

Biophysics　Vol. 44　No. 1　2004/11　Research paper (scientific journal)

Hydrophobic core around tyrosine for human endothelin-1 investigated by photochemically induced dynamic nuclear polarization nuclear magnetic resonance and matrix-assisted laser desorption ionization time-of-flight mass spectrometry

H Takashima, H Tamaoki, N Teno, Y Nishi, S Uchiyama, K Fukui, Y Kobayashi

BIOCHEMISTRY　Vol. 43　No. 44　p. 13932-13936　2004/11　Research paper (scientific journal)

Identification of a novel plant MAR DNA binding protein localized on chromosomal surfaces

Satoru Fujimoto, Sachihiro Matsunaga, Masataka Yonemura, Susumu Uchiyama, Takachika Azuma, Kiichi Fukui

Plant Molecular Biology　Vol. 56　No. 2　p. 225-239　2004/09　Research paper (scientific journal)

Identification of a novel plant MAR DNA binding protein localized on chromosomal surfaces.

Fujimoto S, Matsunaga S, Yonemura M, Uchiyama S, Azuma T, Fukui K

Plant Mol Biol. 56, 225-39.　2004/08　Research paper (scientific journal)

Two states of the triple helix in the thermal transition of the collagen model peptide (Pro-Pro-Gly)(10)

T Kai, S Uchiyama, Y Nishi, Y Kobayashi, T Tomiyama

JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS　Vol. 22　No. 1　p. 51-58　2004/08　Research paper (scientific journal)

Analyses of human chromosomal proteins using monoclonal antibodies

OTsunehito Higashi, Shuichi Miyakawa, Hideaki Takata, Akiko Terauchi, Takeyuki Shimizu, Masayuki Oda, Susumu Uchiyama, Sachihiro Matsunaa, Takachika Azuma, Kiichi Fukui

CELL STRUCTURE AND FUNCTION　Vol. 29　p. 72-72　2004/05

Dynamic analyses of plant chromosomal proteins

Sachihiro Matsunaga, Masataka Yonemura, Akira Kawabe, Satoru Fujimoto, Susumu Uchiyama, Kiichi Fukui

CELL STRUCTURE AND FUNCTION　Vol. 29　p. 72-72　2004/05

Identification of a novel plant MAR DNA binding protein localized on chromosomal surfaces

Satoru Fujimoto, Sachihiro Matsunaga, Masataka Yonemura, Susumu Uchiyama, Takachika Azuma, Kiichi Fukui

CELL STRUCTURE AND FUNCTION　Vol. 29　p. 73-73　2004/05

3P014 Mechanism for protein stabilization in Pseudomonas aeruginosa cytochrome c551 and its variants

Sonoyama T., Uchiyama S., Nakamura S., Oshima A., Kobayashi Y., Sambongi Y.

Seibutsu Butsuri　Vol. 44　2004

3P012 Stability of cytochrome c-552 from a moderate thermophilic bacterium, Hydrogenophilus thermoluteolus

Ichiki S., Nakamura S., Ohshima A., Kobayashi Y., Hasegawa J., Uchiyama S., Nishihara H., Mizuta K., Sanbongi Y.

Seibutsu Butsuri　Vol. 44　2004

3P007 Hydration states of collagen triple helical structures

Nishi Y., Uchiyama S., Doi M., Nishiuchi Y., Nakazawa T., Ohkubo T., Kobayashi Y.

Seibutsu Butsuri　Vol. 44　2004

Cell culture in a closed nano-space

Tomoyuki Doi, Sachihiro Matsunaga, Emi Maeno, Kensuke Tsuchiya, Tsunehito Higashi, Shigeki Misawa, Susumu Uchiyama, Takeshi Ooi, Masayuki Nakao, Kiichi Fukui

Journal of Bioscience and Bioengineering　Vol. 98　No. 4　p. 304-305　2004　Research paper (scientific journal)

Stabilization of triple helical structure of collagen molecule

NAKAZAWA Takashi, Yoshinori Nishi, other people

Peptide Science 2003　p. 25-28　2004　Research paper (international conference proceedings)

Application of the bio-active beads method in rice transformation

Haibo Liu, Akira Kawabe, Sachihiro Matsunaga, Akio Kobayashi, Satoshi Harashima, Susumu Uchiyama, Nobuko Ohmido, Kiichi Fukui

Plant Biotechnology　Vol. 21　No. 4　p. 303-306　2004　Research paper (scientific journal)

Flow karyotypes and chromosomal DNA contents of genus Triticum species and rye (Secale cereale)

JH Lee, YZ Ma, T Wako, LC Li, KY Kim, SW Park, S Uchiyama, K Fukui

CHROMOSOME RESEARCH　Vol. 12　No. 1　p. 93-102　2004　Research paper (scientific journal)

Protein composition of human metaphase chromosomes analyzed by two-dimensional electrophoreses

S Uchiyama, S Kobayashi, H Takata, T Ishihara, T Sone, S Matsunaga, K Fukui

CYTOGENETIC AND GENOME RESEARCH　Vol. 107　No. 1-2　p. 49-54　2004　Research paper (scientific journal)

Synthesis of long Poly(dG)Poly(dC) without structural defects using enzymatic reaction.

Fujiwara, S, Uchiyama, S, Fukui K, Kawai, T, Tanaka, S, Tanaka, H, Taniguchi, M, Tabata, H

ABSTRUCT OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY　2003/12

Synthesis of long strand DNA with specific sequences using enzymatic reaction; Poly(dA)Poly(dT).

Tanaka, SI, Tanaka, H, Taniguchi, M, Tabata, H, Fujiwara, S, Uchiyama, S, Fukui, K, Kawai, T

TABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY　2003/12

CD measurement of aqueous protein solution at high temperature up to 180 °C —Thermodynamic analysis of thermophilic protein by pressure-proof cell compartment

Atsushi Ohshima, Susumu Uchiyama, Hiroaki Nakano, Takuya Yoshida, Tadayasu Ohkubo, Yuji Kobayashi

International Journal of Peptide Research and Therapeutics　Vol. 10　No. 5-6　p. 539-543　2003/11　Research paper (scientific journal)

Structure of super molecules revealed with electron microscopy.

Uchiyama, S, Fukui, K

Chemistry　2003/08

Thermostability of cytochrome c from thermophilic and mesophilic bacteria in oxidized and reduced states

Ohshima,A, Uchiyama,S, Yoshida,T, Ohkubo,T, Sanbongi,Y, Terui,N, Yamamoto,Y, Kobayashi,Y

Biophysics　Vol. 43　No. 1　2003/08　Research paper (scientific journal)

Development of Fusion-inhibiting Anti-HIV-1 Peptides Based on the Structure of Molecular Complex Involved in HIV-1-cell Fusion

Akira Otaka, Miki Nakamura, Eiichi Kodama, Syota Nakamura, Hiroaki Nakano, Susumu Uchiyama, Hirokazu Tamamura, Masao Matsuoka, Yuji Kobayashi, Nobutaka Fujii

Peptide Science 2002　Vol. Vol.2002　No. No.0　p. 65-68　2003/02/01　Research paper (bulletin of university, research institution)

Comparison of the thermal stability on the collagen model peptides containing 4-hydroxyproline and 4-fluoroproline

Nishi Y., Doi M., Uchiyama S., Nishiuchi Y., Nakazawa T., Ohkubo T., Kobayashi Y.

Seibutsu Butsuri　Vol. 43　2003

Stabilization mechanism of triple helical structure of collagen molecules

Y Nishi, M Doi, S Uchiyama, Y Nishiuchi, T Nakazawa, T Ohkubo, Y Kobayashi

LETTERS IN PEPTIDE SCIENCE　Vol. 10　No. 5-6　p. 533-537　2003　Research paper (scientific journal)

Stability and functionality of electron transfer protein cytochrome c from diverse bacteria

Sambongi, Yoshihiro, Ichiki, Shin-ichi, Oikawa, Kenta, Uchiyama, Susumu, Hasegawa, Jun, Terui, Norifumi, Yamamoto, Yasuhiko

ITE Letters on Batteries, New Technologies & Medicine　Vol. 4　No. 5　p. 657-660　2003

Relationship between Redox Function and Protein Stability of Cytochromes c（共著）

山本,泰彦, N.,Terui, N.,Tachiiri, H.,Matsuo, J.,Hasegawa, S.,Uchiyama, Y.,Kobayashi, Y.,Igarashi, Y.,Sambongi

Journal of the American Chemical Society　Vol. 125　No. 45　p. 13650-13651　2003/01　Research paper (scientific journal)

Redox function and thermostability of various P. aeruginosa cytochrome c(551) mutants

Yamamoto, Y., Terui, N., Mita, H., Sambongi, Y., Hemmi, H., Uchiyama, S., Kobayashi, Y., Igarashi, Y., Tachiiri, N., Takayama, SJ

Journal of Inorganic Biochemistry　Vol. 96　No. 1　p. 254-254　2003　Research paper (scientific journal)

CD measurement of aqueous protein solution at high temperature up to 180 degrees C - Thermodynamic analysis of thermophilic protein by pressure-proof cell compartment

A Ohshima, S Uchiyama, H Nakano, Y Takuya, T Ohkubo, Y Kobayashi

LETTERS IN PEPTIDE SCIENCE　Vol. 10　No. 5-6　p. 539-543　2003　Research paper (scientific journal)

Changes in chromosomal surface structure by different isolation conditions

T Sone, M Iwano, S Kobayashi, T Ishihara, N Hori, H Takata, T Ushiki, S Uchiyama, K Fukui

ARCHIVES OF HISTOLOGY AND CYTOLOGY　Vol. 65　No. 5　p. 445-455　2002/12　Research paper (scientific journal)

Simple and efficient syntheses of Boc- and Fmoc-protected 4(R)- and 4(S)-fluoroproline solely from 4(R)-hydroxyproline

M Doi, Y Nishi, N Kiritoshi, T Iwata, M Nago, H Nakano, S Uchiyama, T Nakazawa, T Wakamiya, Y Kobayashi

TETRAHEDRON　Vol. 58　No. 42　p. 8453-8459　2002/10　Research paper (scientific journal)

Letter to editor: Backbone NMR assignments of ribosome recycling factors (RRFs) from Escherichia coli and Thermotoga maritima

T Yoshida, H Kijima, S Oka, S Uchiyama, H Nakano, T Ohkubo, Y Kobayashi

JOURNAL OF BIOMOLECULAR NMR　Vol. 22　No. 2　p. 195-196　2002/02

1I1030 Is the triple helix of (Pro-Pro-Gly)_<10> in a single state in solution?

Kai T., Toyama T., Uchiyama S., Nishi Y., Kobayashi Y.

Seibutsu Butsuri　Vol. 42　No. 2　2002

1I0900 Thermal stability of helix bundle of RRFs from mesophilic and thermophilic bacterium.

Ohshima A., Uchiyama S., Kawachi M., Nakano H., Yoshida T., Ohkubo T., Kobayashi Y.

Seibutsu Butsuri　Vol. 42　No. 2　2002

Remodeling of gp41-C34 peptide leads to highly effective inhibitors of the fusion of HIV-1 with target cells

A. Otaka, M. Nakamura, D. Nameki, E. Kodama, S. Uchiyama, S. Nakamura, H. Nakano, H. Tamamura, Y. Kobayashi, M. Matsuoka, N. Fujii

Angewandte Chemie - International Edition　Vol. 41　No. 16　p. 2938-2940　2002　Research paper (scientific journal)

Solution structure of the ribosome recycling factor from Aquifex aeolicus

T Yoshida, S Uchiyama, H Nakano, H Kashimori, H Kijima, T Ohshima, Y Saihara, T Ishino, H Shimahara, T Yoshida, K Yokose, T Ohkubo, A Kaji, Y Kobayashi

BIOCHEMISTRY　Vol. 40　No. 8　p. 2387-2396　2001/02　Research paper (scientific journal)

Molecular dynamics of RRFs

Oka S., Yoshida T., Nakano H., Uchiyama S., Ohkubo T., Kobayashi Y.

Seibutsu Butsuri　Vol. 41　2001

Synthesis and Characterization of the Collagen Model Peptide Including 4(R)-fluoroproline

Nishi Y, Doi M, Uchiyama S, Nakazawa T, Ohkubo T, Kobayashi Y

Seibutsu Butsuri　Vol. 41　2001

Structure study on a nonfunctional mutant of from RRF E.coli

Moriguchi H, Yoshida T, Uchiyama S, Ohshima A, Nishina K, Nakano H, Ohkubo T, Kobayashi Y

Seibutsu Butsuri　Vol. 41　2001

Relationship between thermal stabilities and activities of ribosome recycling factors from mesophilic and thermophilic bacteria.

Ohshima A, Uchiyama S, Nishina K, Kawachi M, Nakano H, Yoshida T, Ohkubo T, Kobayashi Y

Seibutsu Butsuri　Vol. 41　2001

Correlation of binding affinity and stoichiometry with the size of antigens involved and the segmental flexibility of antibodies

Oda M., Uchiyama S., Kobayashi Y., Azuma T.

Seibutsu Butsuri　Vol. 41　2001

Structural basis of the interaction between IgG and Fcγ receptors

Koichi Kato, Catherine Sautès-Fridman, Wakako Yamada, Kaoru Kobayashi, Susumu Uchiyama, Hahyung Kim, Junichi Enokizono, Annie Galinha, Yuji Kobayashi, Wolf Herman Fridman, Yoji Arata, Ichio Shimada

Journal of Molecular Biology　Vol. 295　No. 2　p. 213-224　2000/01/14　Research paper (scientific journal)

Stability of bacterial cytochrome c studied by differential scanning calorimetry

Uchiyama S., Hasegawa J., Tanimoto Y., Moriguchi H., Mizutani M., Igarashi Y., Sanbongi H., Kobayashi Y.

Seibutsu Butsuri　Vol. 40　2000

Effects of alcohols on the thermal transition of collagen model peptides

Kai T., Nishi Y., Tomiyama T., Uchiyama S., Kobayashi Y.

Seibutsu Butsuri　Vol. 40　2000