Dominant-negative variants in CBX1 cause a neurodevelopmental disorder.

Yukiko Kuroda, Aiko Iwata-Otsubo, Kerith-Rae Dias, Suzanna E L Temple, Koji Nagao, Lachlan De Hayr, Ying Zhu, Shin-Ya Isobe, Gohei Nishibuchi, Sarah K Fiordaliso, Yuki Fujita, Alyssa L Rippert, Samuel W Baker, Marco L Leung, Daniel C Koboldt, Adele Harman, Beth A Keena, Izumi Kazama, Gopinath Musuwadi Subramanian, Kandamurugu Manickam, Betsy Schmalz, Maeson Latsko, Elaine H Zackai, Matt Edwards, Carey-Anne Evans, Matthew C Dulik, Michael F Buckley, Toshihide Yamashita, W Timothy O'Brien, Robert J Harvey, Chikashi Obuse, Tony Roscioli, Kosuke Izumi

Genetics in medicine : official journal of the American College of Medical Genetics　Vol. 25　No. 7　p. 100861-100861　2023/07　Research paper (scientific journal)

Inner nuclear membrane proteins Lem2 and Bqt4 interact with different lipid synthesis enzymes in fission yeast.

Yasuhiro Hirano, Yasuha Kinugasa, Yoshino Kubota, Chikashi Obuse, Tokuko Haraguchi, Yasushi Hiraoka

Journal of biochemistry　2023/02/17　Research paper (scientific journal)

Polycomb repressive complexes 1 and 2 are each essential for maintenance of X inactivation in extra-embryonic lineages.

Osamu Masui, Catherine Corbel, Koji Nagao, Takaho A Endo, Fuyuko Kezuka, Patricia Diabangouaya, Manabu Nakayama, Mami Kumon, Yoko Koseki, Chikashi Obuse, Haruhiko Koseki, Edith Heard

Nature cell biology　Vol. 25　No. 1　p. 134-144　2023/01　Research paper (scientific journal)

RNaseH2A downregulation drives inflammatory gene expression via genomic DNA fragmentation in senescent and cancer cells

Sho Sugawara, Ryo Okada, Tze Mun Loo, Hisamichi Tanaka, Kenichi Miyata, Masatomo Chiba, Hiroko Kawasaki, Kaoru Katoh, Shizuo Kaji, Yoshiro Maezawa, Koutaro Yokote, Mizuho Nakayama, Masanobu Oshima, Koji Nagao, Chikashi Obuse, Satoshi Nagayama, Keiyo Takubo, Akira Nakanishi, Masato T. Kanemaki, Eiji Hara, Akiko Takahashi

Communications Biology　Vol. 5　No. 1　2022/12/28　Research paper (scientific journal)

SmcHD1 underlies the formation of H3K9me3 blocks on the inactive X chromosome in mice

Saya Ichihara, Koji Nagao, Takehisa Sakaguchi, Chikashi Obuse, Takashi Sado

Development　2022/08/01　Research paper (scientific journal)

Chromatin loading of MCM hexamers is associated with di-/tri-methylation of histone H4K20 toward S phase entry.

Yoko Hayashi-Takanaka, Yuichiro Hayashi, Yasuhiro Hirano, Atsuko Miyawaki-Kuwakado, Yasuyuki Ohkawa, Chikashi Obuse, Hiroshi Kimura, Tokuko Haraguchi, Yasushi Hiraoka

Nucleic acids research　Vol. 49　No. 21　p. 12152-12166　2021/12/02　Research paper (scientific journal)

Protein phosphatase 1 acts as a RIF1 effector to suppress DSB resection prior to Shieldin action.

Shin-Ya Isobe, Shin-Ichiro Hiraga, Koji Nagao, Hiroyuki Sasanuma, Anne D Donaldson, Chikashi Obuse

Cell reports　Vol. 36　No. 2　p. 109383-109383　2021/07/13　Research paper (scientific journal)

Mapping replication timing domains genome wide in single mammalian cells with single-cell DNA replication sequencing.

Hisashi Miura, Saori Takahashi, Takahiro Shibata, Koji Nagao, Chikashi Obuse, Katsuzumi Okumura, Masato Ogata, Ichiro Hiratani, Shin-Ichiro Takebayashi

Nature protocols　Vol. 15　No. 12　p. 4058-4100　2020/12　Research paper (scientific journal)

Regulation of an adaptor protein STING by Hsp90β to enhance innate immune responses against microbial infections.

Seiichi Sato, Kai Li, Nozomi Sakurai, Mei Hashizume, Sunanda Baidya, Hirotaka Nonaka, Koki Noguchi, Kozo Ishikawa, Chikashi Obuse, Akinori Takaoka

Cellular immunology　Vol. 356　p. 104188-104188　2020/10　Research paper (scientific journal)

Rare variant of the epigenetic regulator SMCHD1 in a patient with pituitary hormone deficiency.

Kenichi Kinjo, Keisuke Nagasaki, Koji Muroya, Erina Suzuki, Keisuke Ishiwata, Kazuhiko Nakabayashi, Atsushi Hattori, Koji Nagao, Ryu-Suke Nozawa, Chikashi Obuse, Kenji Miyado, Tsutomu Ogata, Maki Fukami, Mami Miyado

Scientific reports　Vol. 10　No. 1　p. 10985-10985　2020/07/03　Research paper (scientific journal)

Homozygous nonsense variant in LRIF1 associated with facioscapulohumeral muscular dystrophy.

Kohei Hamanaka, Darina Šikrová, Satomi Mitsuhashi, Hiroki Masuda, Yukari Sekiguchi, Atsuhiko Sugiyama, Kazumoto Shibuya, Richard J L F Lemmers, Remko Goossens, Megumu Ogawa, Koji Nagao, Chikashi Obuse, Satoru Noguchi, Yukiko K Hayashi, Satoshi Kuwabara, Judit Balog, Ichizo Nishino, Silvère M van der Maarel

Neurology　Vol. 94　No. 23　2020/06/09　Research paper (scientific journal)

Subacute cutaneous lupus erythematosus with melanocyte elimination induced by pembrolizumab.

Mariko Ogawa-Momohara, Yoshinao Muro, Katsunobu Goto, Chikashi Obuse, Minoru Satoh, Michihiro Kono, Masashi Akiyama

The Journal of dermatology　Vol. 47　No. 6　2020/06

KDM2A-dependent reduction of rRNA transcription on glucose starvation requires HP1 in cells, including triple-negative breast cancer cells.

Okamoto K, Tanaka Y, Ogasawara S, Obuse C, Nakayama JI, Yano H, Tsuneoka M

Oncotarget　Vol. 10　No. 46　p. 4743-4760　2019/07　Research paper (scientific journal)

Asymmetrical localization of Nup107-160 subcomplex components within the nuclear pore complex in fission yeast

Haruhiko Asakawa, Gregory P. Copenhaver, Tomoko Kojidani, Hui-Ju Yang, Chizuru Ohtsuki, Hiroko Osakada, Atsushi Matsuda, Masaaki Iwamoto, Yuji Chikashige, Koji Nagao, Chikashi Obuse, Yasushi Hiraoka, Tokuko Haraguchi

PLOS Genetics　Vol. 15　No. 6　2019/06/06　Research paper (scientific journal)

Genome-wide stability of the DNA replication program in single mammalian cells.

Saori Takahashi, Hisashi Miura, Takahiro Shibata, Koji Nagao, Katsuzumi Okumura, Masato Ogata, Chikashi Obuse, Shin-Ichiro Takebayashi, Ichiro Hiratani

Nature genetics　Vol. 51　No. 3　p. 529-540　2019/03　Research paper (scientific journal)

Publisher Correction: Exosomes maintain cellular homeostasis by excreting harmful DNA from cells.

Takahashi A, Okada R, Nagao K, Kawamata Y, Hanyu A, Yoshimoto S, Takasugi M, Watanabe S, Kanemaki MT, Obuse C, Hara E

Nature Communications　Vol. 9　No. 1　2018/10　Research paper (scientific journal)

Role of SmcHD1 in establishment of epigenetic states required for the maintenance of the X-inactivated state in mice.

Sakakibara Y, Nagao K, Blewitt M, Sasaki H, Obuse C, Sado T

Development (Cambridge, England)　Vol. 145　No. 18　2018/09

Functional characterization of lysosomal interaction of Akt with VRK2

Noriyuki Hirata, Futoshi Suizu, Mami Matsuda-Lennikov, Tsutomu Tanaka, Tatsuma Edamura, Satoko Ishigaki, Thoria Donia, Pathrapol Lithanatudom, Chikashi Obuse, Toshihiko Iwanaga, Masayuki Noguchi

Oncogene　p. 1-20　2018/06/05　Research paper (scientific journal)

Nucleosomes around a mismatched base pair are excluded via an Msh2-dependent reaction with the aid of SNF2 family ATPase Smarcad1

Riki Terui, Koji Nagao, Yoshitaka Kawasoe, Kanae Taki, Torahiko L. Higashi, Seiji Tanaka, Takuro Nakagawa, Chikashi Obuse, Hisao Masukata, Tatsuro S. Takahashi

Genes and Development　Vol. 32　No. 11-12　p. 806-821　2018/06/01　Research paper (scientific journal)

Lem2 is retained at the nuclear envelope through its interaction with Bqt4 in fission yeast

Yasuhiro Hirano, Yasuha Kinugasa, Haruhiko Asakawa, Yuji Chikashige, Chikashi Obuse, Tokuko Haraguchi, Yasushi Hiraoka

Genes to Cells　Vol. 23　No. 3　p. 122-135　2018/03/02　Research paper (scientific journal)

分裂酵母に特異的な核膜孔複合体の構造

淺川 東彦, 糀谷 知子, Yang Hui-Ju, 小坂田 裕子, 大槻 千鶴, 長尾 恒治, 小布施 力史, 平岡 泰, 原口 徳子

生命科学系学会合同年次大会　Vol. 2017年度　p. [4LT18-0283)]　2017/12

Ser7 of RNAPII-CTD facilitates heterochromatin formation by linking ncRNA to RNAi

Takuya Kajitani, Hiroaki Kato, Yuji Chikashige, Chihiro Tsutsumi, Yasushi Hiraoka, Hiroshi Kimura, Yasuyuki Ohkawa, Chikashi Obuse, Damien Hermand, Yota Murakami

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　Vol. 114　No. 52　p. E11208-E11217　2017/12　Research paper (scientific journal)

Defects in dosage compensation impact global gene regulation in the mouse trophoblast

Yuka Sakata, Koji Nagao, Yuko Hoki, Hiroyuki Sasaki, Chikashi Obuse, Takashi Sado

DEVELOPMENT　Vol. 144　No. 15　p. 2784-2797　2017/08　Research paper (scientific journal)

Reconstitution of the oocyte nucleolus in mice through a single nucleolar protein, NPM2

Sugako Ogushi, Kazuo Yamagata, Chikashi Obuse, Keiko Furuta, Teruhiko Wakayama, Martin M. Matzuk, Mitinori Saitou

JOURNAL OF CELL SCIENCE　Vol. 130　No. 14　p. 2416-2429　2017/07　Research paper (scientific journal)

Inhibition of RIF1 by SCAI Allows BRCA1-Mediated Repair

Shin-Ya Isobe, Koji Nagao, Naohito Nozaki, Hiroshi Kimura, Chikashi Obuse

CELL REPORTS　Vol. 20　No. 2　p. 297-307　2017/07　Research paper (scientific journal)

Compositionally distinct nuclear pore complexes of functionally distinct dimorphic nuclei in the ciliate Tetrahymena

Masaaki Iwamoto, Hiroko Osakada, Chie Mori, Yasuhiro Fukuda, Koji Nagao, Chikashi Obuse, Yasushi Hiraoka, Tokuko Haraguchi

JOURNAL OF CELL SCIENCE　Vol. 130　No. 10　p. 1822-1834　2017/05　Research paper (scientific journal)

Exosomes maintain cellular homeostasis by excreting harmful DNA from cells

Akiko Takahashi, Ryo Okada, Koji Nagao, Yuka Kawamata, Aki Hanyu, Shin Yoshimoto, Masaki Takasugi, Sugiko Watanabe, Masato T. Kanemaki, Chikashi Obuse, Eiji Hara

NATURE COMMUNICATIONS　Vol. 8　2017/05　Research paper (scientific journal)

Usp7-dependent histone H3 deubiquitylation regulates maintenance of DNA methylation.

Luna Yamaguchi, Atsuya Nishiyama, Toshinori Misaki, Yoshikazu Johmura, Jun Ueda, Kyohei Arita, Koji Nagao, Chikashi Obuse, Makoto Nakanishi

Scientific reports　Vol. 7　No. 1　p. 55-55　2017/03/03　Research paper (scientific journal)

Human RIF1 and protein phosphatase 1 stimulate DNA replication origin licensing but suppress origin activation

Shin-ichiro Hiraga, Tony Ly, Javier Garzon, Zuzana Horejsi, Yoshi-nobu Ohkubo, Akinori Endo, Chikashi Obuse, Simon J. Boulton, Angus I. Lamond, Anne D. Donaldson

EMBO REPORTS　Vol. 18　No. 3　p. 403-419　2017/03　Research paper (scientific journal)

BRCA1 Directs the Repair Pathway to Homologous Recombination by Promoting 53BP1 Dephosphorylation

Mayu Isono, Atsuko Niimi, Takahiro Oike, Yoshihiko Hagiwara, Hiro Sato, Ryota Sekine, Yukari Yoshida, Shin-Ya Isobe, Chikashi Obuse, Ryotaro Nishi, Elena Petricci, Shinichiro Nakada, Takashi Nakano, Atsushi Shibata

CELL REPORTS　Vol. 18　No. 2　p. 520-532　2017/01　Research paper (scientific journal)

The dataset of proteins specifically interacted with activated TICAM-1

Kenji Funami, Misako Matsumoto, Hiroyuki Oshiumi, Chikashi Obuse, Tsukasa Seya

Data in Brief　Vol. 8　p. 697-699　2016/09/01　Research paper (scientific journal)

Corrigendum to "Clinical, muscle pathological, and genetic features of Japanese facioscapulohumeral muscular dystrophy 2 (FSHD2) patients with SMCHD1 mutations": [Neuromuscular Disorders 26/4-5 (2016) 300-308].

Kohei Hamanaka, Kanako Goto, Mami Arai, Koji Nagao, Chikashi Obuse, Satoru Noguchi, Yukiko K Hayashi, Satomi Mitsuhashi, Ichizo Nishino

Neuromuscular disorders : NMD　Vol. 26　No. 7　p. 472-472　2016/07

Histone H3K36 trimethylation is essential for multiple silencing mechanisms in fission yeast

Shota Suzuki, Hiroaki Kato, Yutaka Suzuki, Yuji Chikashige, Yasushi Hiraoka, Hiroshi Kimura, Koji Nagao, Chikashi Obuse, Shinya Takahata, Yota Murakami

NUCLEIC ACIDS RESEARCH　Vol. 44　No. 9　p. 4147-4162　2016/05　Research paper (scientific journal)

14-3-3-zeta participates in TLR3-mediated TICAM-1 signal-platform formation

Kenji Funami, Misako Matsumoto, Chikashi Obuse, Tsukasa Seya

MOLECULAR IMMUNOLOGY　Vol. 73　p. 60-68　2016/05　Research paper (scientific journal)

Clinical, muscle pathological, and genetic features of Japanese facioscapulohumeral muscular dystrophy 2 (FSHD2) patients with SMCHD1 mutations

Kohei Hamanaka, Kanako Goto, Mami Arai, Koji Nagao, Chikashi Obuse, Satoru Noguchi, Yukiko K. Hayashi, Satomi Mitsuhashi, Ichizo Nishino

NEUROMUSCULAR DISORDERS　Vol. 26　No. 4-5　p. 300-308　2016/04　Research paper (scientific journal)

Histone H4 lysine 20 acetylation is associated with gene repression in human cells

Jun-Ya Kaimori, Kazumitsu Maehara, Yoko Hayashi-Takanaka, Akihito Harada, Masafumi Fukuda, Satoko Yamamoto, Naotsugu Ichimaru, Takashi Umehara, Shigeyuki Yokoyama, Ryo Matsuda, Tsuyoshi Ikura, Koji Nagao, Chikashi Obuse, Naohito Nozaki, Shiro Takahara, Toshifumi Takao, Yasuyuki Ohkawa, Hiroshi Kimura, Yoshitaka Isaka

SCIENTIFIC REPORTS　Vol. 6　2016/04　Research paper (scientific journal)

新規核局在化シグナル受容体importin α8の機能解析

宮本 洋一, 盛山 哲嗣, 木本 千裕, 辻井 聡, 五十嵐 芳暢, 小布施 力史, 岡 正啓, 米田 悦啓

日本生化学会大会・日本分子生物学会年会合同大会講演要旨集　Vol. 88回・38回　p. [1T23-14(1P0045)]　2015/12

核内輸送因子Importin αとヒストン結合タンパクRBBP4の非典型的結合とその生理的意義

辻井 聡, 宮本 洋一, 盛山 哲嗣, 土屋 裕子, 小布施 力史, 水口 賢司, 岡 正啓, 米田 悦啓

日本生化学会大会・日本分子生物学会年会合同大会講演要旨集　Vol. 88回・38回　p. [1P0151]-[1P0151]　2015/12

Distribution of histone H4 modifications as revealed by a panel of specific monoclonal antibodies

Yoko Hayashi-Takanaka, Kazumitsu Maehara, Akihito Harada, Takashi Umehara, Shigeyuki Yokoyama, Chikashi Obuse, Yasuyuki Ohkawa, Naohito Nozaki, Hiroshi Kimura

CHROMOSOME RESEARCH　Vol. 23　No. 4　p. 753-766　2015/12　Research paper (scientific journal)

Retinoblastoma-binding Protein 4-regulated Classical Nuclear Transport Is Involved in Cellular Senescence

Akira Tsujii, Yoichi Miyamoto, Tetsuji Moriyama, Yuko Tsuchiya, Chikashi Obuse, Kenji Mizuguchi, Masahiro Oka, Yoshihiro Yoneda

JOURNAL OF BIOLOGICAL CHEMISTRY　Vol. 290　No. 49　p. 29375-29388　2015/12　Research paper (scientific journal)

LRRC59 Regulates Trafficking of Nucleic Acid-Sensing TLRs from the Endoplasmic Reticulum via Association with UNC93B1

Megumi Tatematsu, Kenji Funami, Noriko Ishii, Tsukasa Seya, Chikashi Obuse, Misako Matsumoto

JOURNAL OF IMMUNOLOGY　Vol. 195　No. 10　p. 4933-4942　2015/11　Research paper (scientific journal)

Functional characterization of importin alpha 8 as a classical nuclear localization signal receptor

Chihiro Kimoto, Tetsuji Moriyama, Akira Tsujii, Yoshinobu Igarashi, Chikashi Obuse, Yoichi Miyamoto, Masahiro Oka, Yoshihiro Yoneda

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH　Vol. 1853　No. 10　p. 2676-2683　2015/10　Research paper (scientific journal)

Functional characterization of importin α8 as a classical nuclear localization signal receptor.

Kimoto C, Moriyama T, Tsujii A, Igarashi Y, Obuse C, Miyamoto Y, Oka M, Yoneda Y

Biochimica et biophysica acta　Vol. 1853　No. 10 Pt A　p. 2676-2683　2015/10

HJURP is involved in the expansion of centromeric chromatin

Marinela Perpelescu, Tetsuya Hori, Atsushi Toyoda, Sadahiko Misu, Norikazu Monma, Kazuho Ikeo, Chikashi Obuse, Asao Fujiyama, Tatsuo Fukagawa

MOLECULAR BIOLOGY OF THE CELL　Vol. 26　No. 15　p. 2742-2754　2015/08　Research paper (scientific journal)

Identification and characterization of a nuclear localization signal of TRIM28 that overlaps with the HP1 box

Tetsuji Moriyama, Percival Sangel, Hiroki Yamaguchi, Chikashi Obuse, Yoichi Miyamoto, Masahiro Oka, Yoshihiro Yoneda

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　Vol. 462　No. 3　p. 201-207　2015/07　Research paper (scientific journal)

An annexin A1-FPR1 interaction contributes to necroptosis of keratinocytes in severe cutaneous adverse drug reactions

Nao Saito, Hongjiang Qiao, Teruki Yanagi, Satoru Shinkuma, Keiko Nishimura, Asuka Suto, Yasuyuki Fujita, Shotaro Suzuki, Toshifumi Nomura, Hideki Nakamura, Koji Nagao, Chikashi Obuse, Hiroshi Shimizu, Riichiro Abe

SCIENCE TRANSLATIONAL MEDICINE　Vol. 6　No. 245　2014/07　Research paper (scientific journal)

A novel method for purification of the endogenously expressed fission yeast Set2 complex

Shota Suzuki, Koji Nagao, Chikashi Obuse, Yota Murakami, Shinya Takahata

PROTEIN EXPRESSION AND PURIFICATION　Vol. 97　p. 44-49　2014/05　Research paper (scientific journal)

2SDA-05 Elucidation of construction and function of heterochromatin through HP1 binding proteins(2SDA Studies of dynamic chromatin structure and function to understand fundamentals of life,Symposium,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

Obuse Chikashi

Seibutsu Butsuri　Vol. 54　No. 1　2014

CxxC-ZF Domain Is Needed for KDM2A to Demethylate Histone in rDNA Promoter in Response to Starvation

Yuji Tanaka, Toshiyuki Umata, Kengo Okamoto, Chikashi Obuse, Makoto Tsuneoka

CELL STRUCTURE AND FUNCTION　Vol. 39　No. 1　p. 79-92　2014　Research paper (scientific journal)

Human origin recognition complex binds preferentially to G-quadruplex-preferable RNA and single-stranded DNA

Shoko Hoshina, Kei Yura, Honami Teranishi, Noriko Kiyasu, Ayumi Tominaga, Haruka Kadoma, Ayaka Nakatsuka, Tomoko Kunichika, Chikashi Obuse, Shou Waga

Journal of Biological Chemistry　Vol. 288　No. 42　p. 30161-30171　2013/10/18　Research paper (scientific journal)

Improved and robust detection of cell nuclei from four dimensional fluorescence images

Sato Y, Mukai M, Ueda J, Muraki M, Stasevich T J, Horikoshi N, Kujirai T, Kita H, Kimura T, Hira S, Okada Y, Hayashi-Takanaka Y, Obuse C, Kurumizaka H, Kawahara A, Yamagata K, Nozaki N, Kimura H

Sci Rep　No. 3　2013/08

Vertebrate Spt2 is a novel nucleolar histone chaperone that assists in ribosomal DNA transcription

Akihisa Osakabe, Hiroaki Tachiwana, Motoki Takaku, Tetsuya Hori, Chikashi Obuse, Hiroshi Kimura, Tatsuo Fukagawa, Hitoshi Kurumizaka

JOURNAL OF CELL SCIENCE　Vol. 126　No. 6　p. 1323-1332　2013/03　Research paper (scientific journal)

Genetically encoded system to track histone modification in vivo.

Yuko Sato, Masanori Mukai, Jun Ueda, Michiko Muraki, Timothy J Stasevich, Naoki Horikoshi, Tomoya Kujirai, Hiroaki Kita, Taisuke Kimura, Seiji Hira, Yasushi Okada, Yoko Hayashi-Takanaka, Chikashi Obuse, Hitoshi Kurumizaka, Atsuo Kawahara, Kazuo Yamagata, Naohito Nozaki, Hiroshi Kimura

Scientific reports　Vol. 3　p. 2436-2436　2013　Research paper (scientific journal)

Human inactive X chromosome is compacted through a PRC2-independent SMCHD1-HBiX1 pathway

Ryu-Suke Nozawa, Koji Nagao, Ken-Taro Igami, Sachiko Shibata, Natsuko Shirai, Naohito Nozaki, Takashi Sado, Hiroshi Kimura, Chikashi Obuse

Nature Structural and Molecular Biology　Vol. 20　No. 5　p. 566-573　2013　Research paper (scientific journal)

A co-localization model of paired ChIP-seq data using a large ENCODE data set enables comparison of multiple samples

Kazumitsu Maehara, Jun Odawara, Akihito Harada, Tomohiko Yoshimi, Koji Nagao, Chikashi Obuse, Koichi Akashi, Taro Tachibana, Toshio Sakata, Yasuyuki Ohkawa

NUCLEIC ACIDS RESEARCH　Vol. 41　No. 1　p. 54-62　2013/01　Research paper (scientific journal)

新規ヒストン相互作用因子hsSpt2の核小体クロマチンダイナミクスにおける機能

越阪部 晃永, 立和名 博昭, 高久 誉大, 堀 哲也, 小布施 力史, 木村 宏, 深川 竜郎, 胡桃坂 仁志

日本生化学会大会プログラム・講演要旨集　Vol. 85回　p. 3T22-01　2012/12

Simultaneous inhibition of Src and Aurora kinases by SU6656 induces therapeutic synergy in human synovial sarcoma growth, invasion and angiogenesis in vivo

Ryuta Arai, Masumi Tsuda, Takuya Watanabe, Toyoyuki Ose, Chikashi Obuse, Katsumi Maenaka, Akio Minami, Yusuke Ohba

EUROPEAN JOURNAL OF CANCER　Vol. 48　No. 15　p. 2417-2430　2012/10　Research paper (scientific journal)

Histone chaperone activity of Fanconi anemia proteins, FANCD2 and FANCI, is required for DNA crosslink repair

Koichi Sato, Masamichi Ishiai, Kazue Toda, Satoshi Furukoshi, Akihisa Osakabe, Hiroaki Tachiwana, Yoshimasa Takizawa, Wataru Kagawa, Hiroyuki Kitao, Naoshi Dohmae, Chikashi Obuse, Hiroshi Kimura, Minoru Takata, Hitoshi Kurumizaka

EMBO JOURNAL　Vol. 31　No. 17　p. 3524-3536　2012/08　Research paper (scientific journal)

Epstein-Barr Nuclear Antigen 1 (EBNA1)-dependent Recruitment of Origin Recognition Complex (Orc) on oriP of Epstein-Barr Virus with Purified Proteins STIMULATION BY Cdc6 THROUGH ITS DIRECT INTERACTION WITH EBNA1

Kenji Moriyama, Naoko Yoshizawa-Sugata, Chikashi Obuse, Toshiki Tsurimoto, Hisao Masai

JOURNAL OF BIOLOGICAL CHEMISTRY　Vol. 287　No. 28　p. 23977-23994　2012/07　Research paper (scientific journal)

An inducible RNA interference system in Physcomitrella patens reveals a dominant role of augmin in phragmoplast microtubule generation.

Yuki Nakaoka, Tomohiro Miki, Ryuta Fujioka, Ryota Uehara, Akiko Tomioka, Chikashi Obuse, Minoru Kubo, Yuji Hiwatashi, Gohta Goshima

The Plant cell　Vol. 24　No. 4　p. 1478-93　2012/04

A mutation of the fission yeast EB1 overcomes negative regulation by phosphorylation and stabilizes microtubules

Makoto Iimori, Kanako Ozaki, Yuji Chikashige, Toshiyuki Habu, Yasushi Hiraoka, Takahisa Maki, Ikuko Hayashi, Chikashi Obuse, Tomohiro Matsumoto

EXPERIMENTAL CELL RESEARCH　Vol. 318　No. 3　p. 262-275　2012/02　Research paper (scientific journal)

Prerequisite of sample preparatin for identification of components of a protein complex

NAGAO Koji, NOZAWA Ryu-Suke, OBUSE Chikashi

Abstracts for Annual Meeting of Japanese Proteomics Society　Vol. 2012　p. 78-78　2012

CDK promotes interactions of Sld3 and Drc1 with Cut5 for initiation of DNA replication in fission yeast

Masayoshi Fukuura, Koji Nagao, Chikashi Obuse, Tatsuro S. Takahashi, Takuro Nakagawa, Hisao Masukata

MOLECULAR BIOLOGY OF THE CELL　Vol. 22　No. 14　p. 2620-2633　2011/07　Research paper (scientific journal)

SHP2 Tyrosine Phosphatase Converts Parafibromin/Cdc73 from a Tumor Suppressor to an Oncogenic Driver

Atsushi Takahashi, Ryouhei Tsutsumi, Ippei Kikuchi, Chikashi Obuse, Yasuhiro Saito, Azadeh Seidl, Robert Karisch, Minerva Fernandez, Taewoo Cho, Naomi Ohnishi, Orit Rozenblatt-Rosen, Matthew Meyerson, Benjamin G. Neel, Masanori Hatakeyama

MOLECULAR CELL　Vol. 43　No. 1　p. 45-56　2011/07　Research paper (scientific journal)

Sld7, an Sld3-associated protein required for efficient chromosomal DNA replication in budding yeast

Tamon Tanaka, Toshiko Umemori, Shizuko Endo, Sachiko Muramatsu, Masato Kanemaki, Yoichiro Kamimura, Chikashi Obuse, Hiroyuki Araki

EMBO JOURNAL　Vol. 30　No. 10　p. 2019-2030　2011/05　Research paper (scientific journal)

The initial phase of chromosome condensation requires Cdk1-mediated phosphorylation of the CAP-D3 subunit of condensin II

Satoshi Abe, Kota Nagasaka, Youko Hirayama, Hiroko Kozuka-Hata, Masaaki Oyama, Yutaka Aoyagi, Chikashi Obuse, Toru Hirota

GENES & DEVELOPMENT　Vol. 25　No. 8　p. 863-874　2011/04　Research paper (scientific journal)

Raftlin Is Involved in the Nucleocapture Complex to Induce Poly(I:C)-mediated TLR3 Activation

Ayako Watanabe, Megumi Tatematsu, Kazuko Saeki, Sachiko Shibata, Hiroaki Shime, Akihiko Yoshimura, Chikashi Obuse, Tsukasa Seya, Misako Matsumoto

JOURNAL OF BIOLOGICAL CHEMISTRY　Vol. 286　No. 12　p. 10702-10711　2011/03　Research paper (scientific journal)

Elucidation of construction of epigenetic networks by proteomic approach

OBUSE Chikashi

Abstracts for Annual Meeting of Japanese Proteomics Society　Vol. 2011　p. 59-59　2011

細胞核内のクロマチン空間配置におけるアクチン関連タンパク質Arp6の機能解析

北村 大志, 大渕 恵理, 田辺 秀之, 小布施 力史, 堀 哲也, 深川 竜郎, 原田 昌彦

日本生化学会大会・日本分子生物学会年会合同大会講演要旨集　Vol. 83回・33回　p. 3P-0719　2010/12

Casein kinase 2-dependent phosphorylation of human Rad9 mediates the interaction between human Rad9-Hus1-Rad1 complex and TopBP1

Yukimasa Takeishi, Eiji Ohashi, Kaori Ogawa, Hisao Masai, Chikashi Obuse, Toshiki Tsurimoto

GENES TO CELLS　Vol. 15　No. 7　p. 761-771　2010/07　Research paper (scientific journal)

Human POGZ modulates dissociation of HP1 alpha from mitotic chromosome arms through Aurora B activation

Ryu-Suke Nozawa, Koji Nagao, Hiro-Taka Masuda, Osamu Iwasaki, Toru Hirota, Naohito Nozaki, Hiroshi Kimura, Chikashi Obuse

NATURE CELL BIOLOGY　Vol. 12　No. 7　p. 719-U212　2010/07　Research paper (scientific journal)

Characterization of the interaction of influenza virus NS1 with Akt.

Matsuda M, Suizu F, Hirata N, Miyazaki T, Obuse C, Noguchi M

Biochemical and biophysical research communications　Vol. 395　No. 3　p. 312-317　2010/05

Nucleosome Formation Activity of Human Somatic Nuclear Autoantigenic Sperm Protein (sNASP)

Akihisa Osakabe, Hiroaki Tachiwana, Takaaki Matsunaga, Tatsuya Shiga, Ryu-Suke Nozawa, Chikashi Obuse, Hitoshi Kurumizaka

JOURNAL OF BIOLOGICAL CHEMISTRY　Vol. 285　No. 16　p. 11913-11921　2010/04　Research paper (scientific journal)

Cul8/Rtt101 Forms a Variety of Protein Complexes That Regulate DNA Damage Response and Transcriptional Silencing

Satoru Mimura, Tsuyoshi Yamaguchi, Satoru Ishii, Emiko Noro, Tomoya Katsura, Chikashi Obuse, Takumi Kamura

JOURNAL OF BIOLOGICAL CHEMISTRY　Vol. 285　No. 13　p. 9858-9867　2010/03　Research paper (scientific journal)

Inner centromere formation requires hMis14, a trident kinetochore protein that specifically recruits HP1 to human chromosomes

Tomomi Kiyomitsu, Osamu Iwasaki, Chikashi Obuse, Mitsuhiro Yanagida

JOURNAL OF CELL BIOLOGY　Vol. 188　No. 6　p. 791-807　2010/03　Research paper (scientific journal)

The E3 Ligase TTC3 Facilitates Ubiquitination and Degradation of Phosphorylated Akt

Futoshi Suizu, Yosuke Hiramuki, Fumihiko Okumura, Mami Matsuda, Akiko J. Okumura, Noriyuki Hirata, Masumi Narita, Takashi Kohno, Jun Yokota, Miyuki Bohgaki, Chikashi Obuse, Shigetsugu Hatakeyama, Toshiyuki Obata, Masayuki Noguchi

DEVELOPMENTAL CELL　Vol. 17　No. 6　p. 800-810　2009/12　Research paper (scientific journal)

Schizosaccharomyces pombe cell division cycle under limited glucose requires Ssp1 kinase, the putative CaMKK, and Sds23, a PP2A-related phosphatase inhibitor

Yuichiro Hanyu, Kumiko K. Imai, Yosuke Kawasaki, Takahiro Nakamura, Yukinobu Nakaseko, Koji Nagao, Aya Kokubu, Masahiro Ebe, Asuka Fujisawa, Takeshi Hayashi, Chikashi Obuse, Mitsuhiro Yanagida

GENES TO CELLS　Vol. 14　No. 5　p. 539-554　2009/05　Research paper (scientific journal)

Active establishment of centromeric CENP-A chromatin by RSF complex

Marinela Perpelescu, Naohito Nozaki, Chikashi Obuse, Hua Yang, Kinya Yoda

JOURNAL OF CELL BIOLOGY　Vol. 185　No. 3　p. 397-407　2009/05　Research paper (scientific journal)

The augmin complex plays a critical role in spindle microtubule generation for mitotic progression and cytokinesis in human cells.

Ryota Uehara, Ryu-suke Nozawa, Akiko Tomioka, Sabine Petry, Ronald D Vale, Chikashi Obuse, Gohta Goshima

Proceedings of the National Academy of Sciences of the United States of America　Vol. 106　No. 17　p. 6998-7003　2009/04/28

Involvement of human ORC and TRF2 in pre-replication complex assembly at telomeres

Yasutoshi Tatsumi, Kai Ezura, Kazumasa Yoshida, Takashi Yugawa, Mako Narisawa-Saito, Tohru Kiyono, Satoshi Ohta, Chikashi Obuse, Masatoshi Fujita

GENES TO CELLS　Vol. 13　No. 10　p. 1045-1059　2008/10　Research paper (scientific journal)

Diminishing HDACs by drugs or mutations promotes normal or abnormal sister chromatid separation by affecting APC/C and adherin

Yuu Kimata, Akihisa Matsuyama, Koji Nagao, Kanji Furuya, Chikashi Obuse, Minoru Yoshida, Mitsuhiro Yanagida

JOURNAL OF CELL SCIENCE　Vol. 121　No. 7　p. 1107-1118　2008/04　Research paper (scientific journal)

Human Blinkin/AF15q14 is required for chromosome alignment and the mitotic checkpoint through direct interaction with Bub1 and BubR1

Tomomi Kiyomitsu, Chikashi Obuse, Mitsuhiro Yanagida

DEVELOPMENTAL CELL　Vol. 13　No. 5　p. 663-676　2007/11　Research paper (scientific journal)

alpha-klotho as a regulator of calcium homeostasis

Akihiro Imura, Yoshihito Tsuji, Miyahiko Murata, Ryota Maeda, Koji Kubota, Akiko Iwano, Chikashi Obuse, Kazuya Togashi, Makoto Tominaga, Naoko Kita, Ken-ichi Tomiyama, Junko Iijima, Yoko Nabeshima, Makio Fujioka, Ryo Asato, Shinzo Tanaka, Ken Kojima, Juichi Ito, Kazuhiko Nozaki, Nobuo Hashimoto, Tetsufumi Ito, Takeshi Nishio, Takashi Uchiyama, Toshihiko Fujimori, Yo-ichi Nabeshima

SCIENCE　Vol. 316　No. 5831　p. 1615-1618　2007/06　Research paper (scientific journal)

Helicobacter pylori CagA targets PAR1/MARK kinase to disrupt epithelial cell polarity

Iraj Saadat, Hideaki Higashi, Chikashi Obuse, Mayumi Umeda, Naoko Murata-Kamiya, Yasuhiro Saito, Huaisheng Lu, Naomi Ohnishi, Takeshi Azuma, Atsushi Suzuki, Shigeo Ohno, Masanori Hatakeyama

NATURE　Vol. 447　No. 7142　p. 330-U8　2007/05　Research paper (scientific journal)

Priming of centromere for CENP-A recruitment by human hMis18 alpha, hMis18 beta, and M18BP1

Yohta Fujita, Takeshi Hayashi, Tomomi Kiyomitsu, Yusuke Toyoda, Aya Kokubu, Chikashi Obuse, Mitsuhiro Yanagida

DEVELOPMENTAL CELL　Vol. 12　No. 1　p. 17-30　2007/01　Research paper (scientific journal)

Comprehensive analysis of the ICEN (Interphase Centromere Complex) components enriched in the CENP-A chromatin of human cells

H Izuta, M Ikeno, N Suzuki, T Tomonaga, N Nozaki, C Obuse, Y Kisu, N Goshima, F Nomura, N Nomura, K Yoda

GENES TO CELLS　Vol. 11　No. 6　p. 673-684　2006/06　Research paper (scientific journal)

Two E3 ubiquitin ligases, SCF-Skp2 and DDB1-Cul4, target human Cdt1 for proteolysis

H Nishitani, N Sugimoto, Roukos, V, Y Nakanishi, M Saijo, C Obuse, T Tsurimoto, KI Nakayama, K Nakayama, M Fujita, Z Lygerou, T Nishimoto

EMBO JOURNAL　Vol. 25　No. 5　p. 1126-1136　2006/03　Research paper (scientific journal)

A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1

C Obuse, O Iwasaki, T Kiyomitsu, G Goshima, Y Toyoda, M Yanagida

NATURE CELL BIOLOGY　Vol. 6　No. 11　p. 1135-U37　2004/11　Research paper (scientific journal)

The reconstituted human Chl12-RFC complex functions as a second PCNA loader

Y Shiomi, A Shinozaki, K Sugimoto, J Usukura, C Obuse, T Tsurimoto

GENES TO CELLS　Vol. 9　No. 4　p. 279-290　2004/04　Research paper (scientific journal)

Proteomics analysis of the centromere complex from HeLa interphase cells: UV-damaged DNA binding protein 1 (DDB-1) is a component of the CEN-complex, while BMI-1 is transiently co-localized with the centromeric region in interphase

C Obuse, H Yang, N Nozaki, SH Goto, T Okazaki, K Yoda

GENES TO CELLS　Vol. 9　No. 2　p. 105-120　2004/02　Research paper (scientific journal)

The ORC1 cycle in human cells - II. Dynamic changes in the human ORC complex during the cell cycle

S Ohta, Y Tatsumi, M Fujita, T Tsurimoto, C Obuse

JOURNAL OF BIOLOGICAL CHEMISTRY　Vol. 278　No. 42　p. 41535-41540　2003/10　Research paper (scientific journal)

The ORC1 cycle in human cells - I. Cell cycle-regulated oscillation of human ORC1

Y Tatsumi, S Ohta, H Kimura, T Tsurimoto, C Obuse

JOURNAL OF BIOLOGICAL CHEMISTRY　Vol. 278　No. 42　p. 41528-41534　2003/10　Research paper (scientific journal)

The role of Ppe1/PP6 phosphatase for equal chromosome segregation in fission yeast kinetochore

G Goshima, O Iwasaki, C Obuse, M Yanagida

EMBO JOURNAL　Vol. 22　No. 11　p. 2752-2763　2003/06　Research paper (scientific journal)

A proteomics approach to identify proliferating cell nuclear antigen (PCNA)-binding proteins in human cell lysates - Identification of the human CHL12/RFCs2-5 complex as a novel PCNA-binding protein

S Ohta, Y Shiomi, K Sugimoto, C Obuse, T Tsurimoto

JOURNAL OF BIOLOGICAL CHEMISTRY　Vol. 277　No. 43　p. 40362-40367　2002/10　Research paper (scientific journal)

PCNA clamp facilitates action of DNA cytosine methyltransferase 1 on hemimethylated DNA

T Iida, Suetake, I, S Tajima, H Morioka, S Ohta, C Obuse, T Tsurimoto

GENES TO CELLS　Vol. 7　No. 10　p. 997-1007　2002/10　Research paper (scientific journal)

Clamp and clamp loader structures of the human checkpoint protein complexes, Rad9-1-1 and Rad17-RFC

Y Shiomi, A Shinozaki, D Nakada, K Sugimoto, J Usukura, C Obuse, T Tsurimoto

GENES TO CELLS　Vol. 7　No. 8　p. 861-868　2002/08　Research paper (scientific journal)

Scheduled conversion of replication complex architecture at replication origins of Saccharomyces cerevisiae during the cell cycle

R Tadokoro, M Fujita, H Miura, K Shirahige, H Yoshikawa, T Tsurimoto, C Obuse

JOURNAL OF BIOLOGICAL CHEMISTRY　Vol. 277　No. 18　p. 15881-15889　2002/05　Research paper (scientific journal)

The human homologue of fission yeast cdc27, p66, is a component of active human DNA polymerase delta

K Shikata, S Ohta, K Yamada, C Obuse, H Yoshikawa, T Tsurimoto

JOURNAL OF BIOCHEMISTRY　Vol. 129　No. 5　p. 699-708　2001/05　Research paper (scientific journal)

ATP-dependent structural change of the eukaryotic clamp-loader protein, replication factor C

Y Shiomi, J Usukura, Y Masamura, K Takeyasu, Y Nakayama, C Obuse, H Yoshikawa, T Tsurimoto

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　Vol. 97　No. 26　p. 14127-14132　2000/12　Research paper (scientific journal)

Association of human origin recognition complex 1 with chromatin DNA and nuclease-resistant nuclear structures

Y Tatsumi, T Tsurimoto, K Shirahige, H Yoshikawa, C Obuse

JOURNAL OF BIOLOGICAL CHEMISTRY　Vol. 275　No. 8　p. 5904-5910　2000/02　Research paper (scientific journal)

Cell cycle dependent topological changes of chromosomal replication origins in Saccharomyces cerevisiae

M Fujita, Y Hori, K Shirahige, T Tsurimoto, H Yoshikawa, C Obuse

GENES TO CELLS　Vol. 3　No. 11　p. 737-749　1998/11　Research paper (scientific journal)

Regulation of DNA-replication origins during cell-cycle progression

K Shirahige, Y Hori, K Shiraishi, M Yamashita, K Takahashi, C Obuse, T Tsurimoto, H Yoshikawa

NATURE　Vol. 395　No. 6702　p. 618-621　1998/10　Research paper (scientific journal)

Interaction of transcription factor YY1 with a replication-enhancing element, REE1, in an autonomously replicating human chromosome fragment

C Obuse, T Okazaki, H Masukata

NUCLEIC ACIDS RESEARCH　Vol. 26　No. 10　p. 2392-2397　1998/05　Research paper (scientific journal)

The efficiency and timing of initiation of replication of multiple replicons of Saccharomyces cerevisiae chromosome VI

M Yamashita, Y Hori, T Shinomiya, C Obuse, T Tsurimoto, H Yoshikawa, K Shirahige

GENES TO CELLS　Vol. 2　No. 11　p. 655-665　1997/11　Research paper (scientific journal)

Characterization of a novel CDC gene (ORC1) partly homologous to CDC6 of Saccharomyces cerevisiae

Y Hori, K Shirahige, C Obuse, T Tsurimoto, H Yoshikawa

MOLECULAR BIOLOGY OF THE CELL　Vol. 7　No. 3　p. 409-418　1996/03　Research paper (scientific journal)

A replication-enhancing element with transcriptional silencer activity in autonomously replicating human chromosomal fragments

C Obuse, Y Okuno, T Okazaki, H Masukata

MOLECULAR BIOLOGY OF THE CELL　Vol. 7　No. 1　p. 43-55　1996/01　Research paper (scientific journal)

AUTONOMOUS REPLICATION OF HUMAN CHROMOSOMAL DNA FRAGMENTS IN HUMAN-CELLS

H MASUKATA, H SATOH, C OBUSE, T OKAZAKI

MOLECULAR BIOLOGY OF THE CELL　Vol. 4　No. 11　p. 1121-1132　1993/11　Research paper (scientific journal)