Force detection of high-frequency electron paramagnetic resonance spectroscopy of microliter solution sample

Okamoto Tsubasa, Takahashi Hideyuki, Ohmichi Eiji, Ishikawa Haruto, Mizutani Yasuhisa, Ohta Hitoshi

APPLIED PHYSICS LETTERS　Vol. 113　No. 22　p. 223702-223702　2018/11/26　Research paper (scientific journal)

Potent Antimalarial Activity of Two Arenes Linked with Triamine Designed To Have Multiple Interactions with Heme.

Yosuke Sakata, Kosuke Yabunaka, Yuko Kobayashi, Hirohisa Omiya, Naoki Umezawa, Hye-Sook Kim, Yusuke Wataya, Yoshimi Tomita, Yosuke Hisamatsu, Nobuki Kato, Hirokazu Yagi, Tadashi Satoh, Koichi Kato, Haruto Ishikawa, Tsunehiko Higuchi

ACS medicinal chemistry letters　Vol. 9　No. 10　p. 980-985　2018/10/11　Research paper (scientific journal)

Redox-dependent axial ligand replacement and its functional significance in heme-bound iron regulatory proteins

Mariko Ogura, Ryosuke Endo, Haruto Ishikawa, Yukiko Takeda, Takeshi Uchida, Kazuhiro Iwai, Kazuo Kobayashi, Koichiro Ishimori

Journal of Inorganic Biochemistry　Vol. 182　p. 238-248　2018/05/01　Research paper (scientific journal)

Tertiary dynamics of human adult hemoglobin fixed in R and T quaternary structures

Shanyan Chang, Misao Mizuno, Haruto Ishikawa, Yasuhisa Mizutani

Physical Chemistry Chemical Physics　Vol. 20　No. 5　p. 3363-3372　2018/02/07　Research paper (scientific journal)

A Study of the Dynamics of the Heme Pocket and C-helix in CooA upon CO Dissociation Using Time-Resolved Visible and UV Resonance Raman Spectroscopy

Akihiro Otomo, Haruto Ishikawa, Misao Mizuno, Tetsunari Kimura, Minoru Kubo, Yoshitsugu Shiro, Shigetoshi Aono, Yasuhisa Mizutani

JOURNAL OF PHYSICAL CHEMISTRY B　Vol. 120　No. 32　p. 7836-7843　2016/08　Research paper (scientific journal)

Regulatory Implications of Structural Changes in Tyr201 of the Oxygen Sensor Protein FixL

Takeo Yamawaki, Haruto Ishikawa, Misao Mizuno, Hiro Nakamura, Yoshitsugu Shiro, Yasuhisa Mizutani

BIOCHEMISTRY　Vol. 55　No. 29　p. 4027-4035　2016/07　Research paper (scientific journal)

Redox-Dependent Dynamics in Heme-Bound Bacterial Iron Response Regulator (Irr) Protein

Kazuo Kobayashi, Megumi Nakagaki, Haruto Ishikawa, Kazuhiro Iwai, Mark R. O'Brian, Koichiro Ishimori

BIOCHEMISTRY　Vol. 55　No. 29　p. 4047-4054　2016/07　Research paper (scientific journal)

Effect of the N-terminal residues on the quaternary dynamics of human adult hemoglobin

Shanyan Chang, Misao Mizuno, Haruto Ishikawa, Yasuhisa Mizutani

CHEMICAL PHYSICS　Vol. 469　p. 31-37　2016/05　Research paper (scientific journal)

Carbon monoxide binding properties of domain-swapped dimeric myoglobin

Satoshi Nagao, Haruto Ishikawa, Takuya Yamada, Yasuhisa Mizutani, Shun Hirota

JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY　Vol. 20　No. 3　p. 523-530　2015/04　Research paper (scientific journal)

Vibrational Energy Flow in Hemeproteins

Yasuhisa Mizutani, Naoki Fujii, Mitsuhiro Miyamoto, Misao Mizuno, Haruto Ishikawa

ULTRAFAST PHENOMENA XIX　Vol. 162　p. 532-534　2015　Research paper (international conference proceedings)

Observing Vibrational Energy Flow in a Protein with the Spatial Resolution of a Single Amino Acid Residue

Naoki Fujii, Misao Mizuno, Haruto Ishikawa, Yasuhisa Mizutani

JOURNAL OF PHYSICAL CHEMISTRY LETTERS　Vol. 5　No. 18　p. 3269-3273　2014/09　Research paper (scientific journal)

Identification of Essential Histidine Residues Involved in Heme Binding and Hemozoin Formation in Heme Detoxification Protein from Plasmodium falciparum

Keisuke Nakatani, Haruto Ishikawa, Shigetoshi Aono, Yasuhisa Mizutani

SCIENTIFIC REPORTS　Vol. 4　2014/08　Research paper (scientific journal)

2P094 Protein-based FRET sensor for oxygen concentration(02. Heme proteins,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

Ishikawa Haruto, Aono Shigetoshi, Mizutani Yasuhisa

Seibutsu Butsuri　Vol. 54　No. 1　2014

2P095 Development of the FRET-based sensor protein for visualization of free heme in vivo(02. Heme proteins,Poster,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

Matsumoto Kazuyuki, Ishikawa Haruto, Aono Shigetoshi, Mizutani Yasuhisa

Seibutsu Butsuri　Vol. 54　No. 1　2014

Vibrational energy flow in hemeproteins

Mizutani Y, Fujii N, Miyamoto M, Mizuno M, Ishikawa H

Optics InfoBase Conference Papers　2014

Ultraviolet Resonance Raman Observations of the Structural Dynamics of Rhizobial Oxygen Sensor FixL on Ligand Recognition

Shinji Yano, Haruto Ishikawa, Misao Mizuno, Hiro Nakamura, Yoshitsugu Shiro, Yasuhisa Mizutani

JOURNAL OF PHYSICAL CHEMISTRY B　Vol. 117　No. 49　p. 15786-15791　2013/12　Research paper (scientific journal)

Intersubunit Communication via Changes in Hemoglobin Quaternary Structures Revealed by Time-Resolved Resonance Raman Spectroscopy: Direct Observation of the Perutz Mechanism

Kenta Yamada, Haruto Ishikawa, Misao Mizuno, Naoya Shibayama, Yasuhisa Mizutani

JOURNAL OF PHYSICAL CHEMISTRY B　Vol. 117　No. 41　p. 12461-12468　2013/10　Research paper (scientific journal)

Heme-binding properties of heme detoxification protein from Plasmodium falciparum

Keisuke Nakatani, Haruto Ishikawa, Shigetoshi Aono, Yasuhisa Mizutani

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　Vol. 439　No. 4　p. 477-480　2013/10　Research paper (scientific journal)

2P099 Protein dynamics of CooA upon CO dissociation studied by time-resolved resonance Raman spectroscopy(02. Heme proteins,Poster)

Otomo Akihiro, Ishikawa Haruto, Mizuno Misao, Aono Shigetoshi, Mizutani Yasuhisa

Seibutsu Butsuri　Vol. 53　No. 1　2013

2P098 Heme binding site in Rhodnius prolixus α-glucosidase promoting the heme crystallization(02. Heme proteins,Poster)

Kaku Shotaro, Nakatani Keisuke, Ishikawa Haruto, Mizutani Yasuhisa

Seibutsu Butsuri　Vol. 53　No. 1　2013

Structural dynamics of proximal heme pocket in HemAT-Bs associated with oxygen dissociation

Yuu Yoshida, Haruto Ishikawa, Shigetoshi Aono, Yasuhisa Mizutani

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS　Vol. 1824　No. 7　p. 866-872　2012/07　Research paper (scientific journal)

Protein Dynamics of Isolated Chains of Recombinant Human Hemoglobin Elucidated by Time-Resolved Resonance Raman Spectroscopy

Kenta Yamada, Haruto Ishikawa, Yasuhisa Mizutani

JOURNAL OF PHYSICAL CHEMISTRY B　Vol. 116　No. 6　p. 1992-1998　2012/02　Research paper (scientific journal)

Structural basis for oxygen sensing and signal transduction of the heme-based sensor protein Aer2 from Pseudomonas aeruginosa

Hitomi Sawai, Hiroshi Sugimoto, Yoshitsugu Shiro, Haruto Ishikawa, Yasuhisa Mizutani, Shigetoshi Aono

CHEMICAL COMMUNICATIONS　Vol. 48　No. 52　p. 6523-6525　2012　Research paper (scientific journal)

Unusual Heme Binding in the Bacterial Iron Response Regulator Protein: Spectral Characterization of Heme Binding to the Heme Regulatory Motif

Haruto Ishikawa, Megumi Nakagaki, Ai Bamba, Takeshi Uchida, Hiroshi Hori, Mark R. O'Brian, Kazuhiro Iwai, Koichiro Ishimori

BIOCHEMISTRY　Vol. 50　No. 6　p. 1016-1022　2011/02　Research paper (scientific journal)

PHYS 430-Enzyme dynamical changes upon substrate binding and structural interconversions measured with ultrafast 2-D IR vibrational echo spectroscopy

Michael D. Fayer, Haruto Ishikawa, Ilya J. Finkelstein, Jean K. Chung, Kyungwon Kwak, Seongheun Kim

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY　Vol. 237　2009/03

Ultrafast 2D-IR vibration echo spectroscopy of proteins

Ishikawa, H., Kim, S., Finkelstein, I.J., Fayer, M.D.

Advances in Biomedical Spectroscopy　Vol. 2　p. 79-103　2009　Research paper (scientific journal)

Direct observation of fast protein conformational switching

Haruto Ishikawa, Kyungwon Kwak, Jean K. Chung, Seongheun Kim, Michael D. Fayer

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　Vol. 105　No. 25　p. 8619-8624　2008/06　Research paper (scientific journal)

Disulfide bond influence on protein structural dynamics probed with 2D-IR vibrational echo spectroscopy

Haruto Ishikawa, Seongheun Kim, Kyungwon Kwak, Keisuke Wakasugi, Michael D. Fayer

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　Vol. 104　No. 49　p. 19309-19314　2007/12　Research paper (scientific journal)

Neuroglobin dynamics observed with ultrafast 2D-IR vibrational echo spectroscopy

Haruto Ishikawa, Ilya J. Finkelstein, Seongheun Kim, Kyungwon Kwak, Jean K. Chung, Keisuke Wakasugi, Aaron M. Massari, Michael D. Fayer

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　Vol. 104　No. 41　p. 16116-16121　2007/10　Research paper (scientific journal)

Influence of environment and substrate binding on the dynamics of heme proteins

Ilya J. Finkelstein, Haruto Ishikawa, Seongheun Kim, Michael D. Fayer

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY　Vol. 233　2007/03

Substrate binding and protein conformational dynamics measured by 2D-IR vibrational echo spectroscopy

Ilya J. Finkelstein, Haruto Ishikawa, Seongheun Kim, Aaron M. Massari, M. D. Fayer

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA　Vol. 104　No. 8　p. 2637-2642　2007/02　Research paper (scientific journal)

Probing dynamics of complex molecular systems with ultrafast 2D IR vibrational echo spectroscopy

Ilya J. Finkelstein, Junrong Zheng, Haruto Ishikawa, Seongheun Kim, Kyungwon Kwak, Michael D. Fayer

PHYSICAL CHEMISTRY CHEMICAL PHYSICS　Vol. 9　No. 13　p. 1533-1549　2007

Mechanism underlying ubiquitination of iron regulatory protein 2 (IRP2)

Iwai, K., Ishikawa, H.

Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme.　Vol. 51　No. 10 Suppl　p. 1287-1291　2006　Research paper (scientific journal)

Involvement of heme regulatory motif in heme-mediated ubiquitination and degradation of IRP2

H Ishikawa, M Kato, H Hori, K Ishimori, T Kirisako, F Tokunaga, K Iwai

MOLECULAR CELL　Vol. 19　No. 2　p. 171-181　2005/07　Research paper (scientific journal)

3P104 Spectroscopic Characterization of Heme Coordination Structure in Iron Regulatory Protein 2 (IRP2)

Endo R., Ishikawa H., Uchida T., Kobayashi K., Kitagawa T., Iwai K., Ishimori K.

Seibutsu Butsuri　Vol. 45　2005

3P081 Heme Binding in Iron Response Regulator (Irr) and its Regulation Mechanism

Nakagaki M., Uchida T., Kobayashi K., Ishikawa H., Kitagawa H., Iwai K., O'Brian Mark, Ishimori K.

Seibutsu Butsuri　Vol. 45　2005

Identification, expression, and assay of an oxidation-specific ubiquitin ligase, HOIL-1

K Iwai, H Ishikawa, T Kirisako

UBIQUITIN AND PROTEIN DEGRADATION, PART A　Vol. 398　p. 256-271　2005　Part of collection (book)

Steric effects of isoleucine 107 on heme reorientation reaction in human myoglobin

H Ishikawa, S Takahashi, K Ishimori, Morishima, I

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS　Vol. 324　No. 3　p. 1095-1100　2004/11　Research paper (scientific journal)

Identification of crucial histidines for heme binding in the N-terminal domain of the heme-regulated eIF2 alpha kinase

T Inuzuka, BG Yun, H Ishikawa, S Takahashi, H Hori, RL Matts, K Ishimori, Morishima, I

JOURNAL OF BIOLOGICAL CHEMISTRY　Vol. 279　No. 8　p. 6778-6782　2004/02　Research paper (scientific journal)

2P062 Heme Binding Affinity of Iron Response Regulator (Irr) and its Regulation Mechanism

Nakagaki M., Bamba A., Ishikawa H., Iwai K., O'Brian Mark, Morishima I., Is K.

Seibutsu Butsuri　Vol. 44　2004

2P063 Spectroscopic Characterization of Heme Binding and Coordination Structure in Iron Regulatory Protein 2(IRP2)

Endo R., Ishikawa H., Iwai K., Morishima I., Ishimori K.

Seibutsu Butsuri　Vol. 44　2004

Identification of the ubiquitin-protein ligase that recognizes oxidized IRP2

K Yamanaka, H Ishikawa, Y Megumi, F Tokunaga, M Kanie, TA Rouault, Morishima, I, N Minato, K Ishimori, K Iwai

NATURE CELL BIOLOGY　Vol. 5　No. 4　p. 336-340　2003/04　Research paper (scientific journal)

Iron hemiporphycene as a functional prosthetic group for myoglobin

S Neya, K Imai, H Hori, H Ishikawa, K Ishimori, D Okuno, S Nagatomo, T Hoshino, M Hata, N Funasaki

INORGANIC CHEMISTRY　Vol. 42　No. 5　p. 1456-1461　2003/03　Research paper (scientific journal)

NO-induced activation mechanism of the heme-regulated eIF2 alpha kinase

H Ishikawa, BG Yun, S Takahashi, H Hori, RL Matts, K Ishimori, Morishima, I

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY　Vol. 124　No. 46　p. 13696-13697　2002/11　Research paper (scientific journal)

Binding of CO at the Pro(2) side is crucial for the activation of CO-sensing transcriptional activator CooA - H-1 NMR spectroscopic studies

K Yamamoto, H Ishikawa, S Takahashi, K Ishimori, Morishima, I, H Nakajima, S Aono

JOURNAL OF BIOLOGICAL CHEMISTRY　Vol. 276　No. 15　p. 11473-11476　2001/04　Research paper (scientific journal)

Ligand migration in human myoglobin: Steric effects of isoleucine 107(G8) on O-2 and CO binding

H Ishikawa, T Uchida, S Takahashi, K Ishimori, Morishima, I

BIOPHYSICAL JOURNAL　Vol. 80　No. 3　p. 1507-1517　2001/03　Research paper (scientific journal)

Ligand migration in human myoglobin: Steric effects of isoleucine 107(G8) on O-2 and CO binding

H Ishikawa, T Uchida, S Takahashi, K Ishimori, Morishima, I

BIOPHYSICAL JOURNAL　Vol. 80　No. 3　p. 1507-1517　2001/03　Research paper (scientific journal)

Time-resolved hole-burning study on myoglobin: Fluctuation of restricted water within distal pocket

Y Shibata, H Ishikawa, S Takahashi, Morishima, I

BIOPHYSICAL JOURNAL　Vol. 80　No. 2　p. 1013-1023　2001/02　Research paper (scientific journal)

Functional difference between IRE-recongnation and IRE-binding sites in IRPs

Watanabe K., Ishikawa H., Iwai K., Ishimori K., Morishima I.

Seibutsu Butsuri　Vol. 41　2001

Study on DNA-binding Activity of Heme-containing Transcriptional Regulator CooA by Using Fluorescence Anisotropy

Yamamoto K., Ishikawa H., Ishimori K., Morishima I., Nakajima H., Aono S.

Seibutsu Butsuri　Vol. 40　2000

2PA087 ヘモグロビンβ鎖の疎水性残基Leu28による配位子結合反応の制御に関する研究

小西 一誠, 石川 春人, 近藤 詩乃, 高橋 聡, 石森 浩一郎, 森島 績

生物物理　Vol. 39　1999

2PA094 転写調節因子CooAの機能発現に関与するヘム軸配位子の研究

山本 克彦, 石川 春人, 高橋 聡, 石森 浩一郎, 森島 績, 中島 洋, 青野 重利

生物物理　Vol. 39　1999

Heme environmental structure of CooA is modulated by the target DNA binding

T Uchida, H Ishikawa, S Takahashi, K Ishimori, Morishima, I, K Ohkubo, H Nakajima, S Aono

JOURNAL OF BIOLOGICAL CHEMISTRY　Vol. 273　No. 32　p. 19988-19992　1998/08　Research paper (scientific journal)