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Nakai Masato

中井 正人

Institute for Protein Research, Associate Professor

Education

  • - 1990, Osaka University
  • - 1990, Osaka University, Graduate School, Division of Natural Science
  • - 1985, Osaka University, School of Science
  • - 1985, Osaka University, Faculty of Science

Research History

  • 1990 - 1996, Nagoya University
  • 1990 - 1996, Nagoya University, Assistant Professor

Research Areas

  • Life sciences, Cell biology
  • Life sciences, Molecular biology
  • Life sciences, Functional biochemistry
  • Life sciences, Structural biochemistry
  • Life sciences, Plants: molecular biology and physiology

Professional Memberships

  • 日本細胞生物学会
  • 蛋白質科学会
  • American Society of Plant Biologists
  • The American Association for the Advancement of Science
  • 植物生理学会
  • 分子生物学会
  • 生化学会

Papers

  • The Revised Model for Chloroplast Protein Import, Masato Nakai, The Plant Cell, Vol. 32, No. 3, p. 543-546, 2020/03
  • tRNA Wobble Modification Affects Leaf Cell Development in Arabidopsis thaliana., Yumi Nakai,Gorou Horiguchi,Kosei Iwabuchi,Akiko Harada,Masato Nakai,Ikuko Hara-Nishimura,Takato Yano, Plant & cell physiology, Vol. 60, No. 9, p. 2026-2039, 2019/09/01
  • A Ycf2-FtsHi heteromeric AAA-ATPase complex is required for chloroplast protein import, Shingo Kikuchi,Yukari Asakura,Midori Imai,Yoichi Nakahira,Yoshiko Kotani,Yasuyuki Hashiguchi,Yumi Nakai,Kazuaki Takafuji,Jocelyn B_dard,Yoshino Hirabayashi-Ishioka,Hitoshi Mori,Takashi Shiina,Masato Nakai, The Plant Cell, Vol. Breakthrough Report, No. in press, 2018/10/12
  • New perspectives on chloroplast protein import, Masato Nakai, Plant and Cell Physiology, Oxford University Press, Vol. 59, No. 6, p. 1111-1119, 2018/06/01
  • Sulfur modifications of the Wobble U34 in tRNAs and their intracellular localization in eukaryotic cells, Yumi Nakai,Masato Nakai,Takato Yano, Biomolecules, MDPI AG, Vol. 7, No. 1, 2017/02/18
  • The TIC complex uncovered: The alternative view on the molecular mechanism of protein translocation across the inner envelope membrane of chloroplasts, Masato Nakai, BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, ELSEVIER SCIENCE BV, Vol. 1847, No. 9, p. 957-967, 2015/09
  • Chloroplast envelope localization of EDS5, an essential factor for salicylic acid biosynthesis in arabidopsis thaliana, Kanako Yamasaki,Yukiya Motomura,Yusuke Yagi,Hironari Nomura,Shingo Kikuchi,Masato Nakai,Takashi Shiina, Plant Signaling and Behavior, Vol. 8, No. 4, p. e236031-e236035, 2013/04
  • Uncovering the Protein Translocon at the Chloroplast Inner Envelope Membrane, Shingo Kikuchi,Jocelyn Bedard,Minako Hirano,Yoshino Hirabayashi,Maya Oishi,Midori Imai,Mai Takase,Toru Ide,Masato Nakai, SCIENCE, AMER ASSOC ADVANCEMENT SCIENCE, Vol. 339, No. 6119, p. 571-574, 2013/02
  • Both phototropin 1 and 2 localize on the chloroplast outer membrane with distinct localization activity, Sam-Geun Kong,Noriyuki Suetsugu,Shingo Kikuchi,Masato Nakai,Akira Nagatani,Masamitsu Wada, Plant and Cell Physiology, Vol. 54, No. 1, p. 80-92, 2013/01
  • Arabidopsis Molybdopterin Biosynthesis Protein Cnx5 Collaborates with the Ubiquitin-like Protein Urm11 in the Thio-modification of tRNA, Yumi Nakai,Akiko Harada,Yasuyuki Hashiguchi,Masato Nakai,Hideyuki Hayashi, JOURNAL OF BIOLOGICAL CHEMISTRY, AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, Vol. 287, No. 36, p. 30874-30884, 2012/08
  • In Vivo Studies on the Roles of Two Closely Related Arabidopsis Tic20 Proteins, AtTic20-I and AtTic20-IV, Yoshino Hirabayashi,Shingo Kikuchi,Maya Oishi,Masato Nakai, PLANT AND CELL PHYSIOLOGY, OXFORD UNIV PRESS, Vol. 52, No. 3, p. 469-478, 2011/03
  • One- and two-dimensional blue native-PAGE and immunodetection of low-abundance chloroplast membrane protein complexes, Shingo Kikuchi,Jocelyn Bédard,Masato Nakai, Methods in Molecular Biology, Vol. 775, p. 3-17, 2011
  • A 1-Megadalton Translocation Complex Containing Tic20 and Tic21 Mediates Chloroplast Protein Import at the Inner Envelope Membrane, Shingo Kikuchi,Maya Oishi,Yoshino Hirabayashi,Dong Wook Lee,Inhwan Hwang,Masato Nakai, PLANT CELL, AMER SOC PLANT BIOLOGISTS, Vol. 21, No. 6, p. 1781-1797, 2009/06
  • Arabidopsis cytosolic Nbp35 homodimer can assemble both [2Fe-2S] and [4Fe-4S] clusters in two distinct domains, Hirokazu Kohbushi,Yumi Nakai,Shingo Kikuchi,Toshiki Yabe,Hiroshi Hori,Masato Nakai, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC ELSEVIER SCIENCE, Vol. 378, No. 4, p. 810-815, 2009/01
  • Non-identical contributions of two membrane-bound cpSRP components, cpFtsY and Alb3, to thylakoid biogenesis, Yukari Asakura,Shingo Kikuchi,Masato Nakai, PLANT JOURNAL, WILEY-BLACKWELL, Vol. 56, No. 6, p. 1007-1017, 2008/12
  • Thio-modification of yeast cytosolic tRNA requires a ubiquitin-related system that resembles bacterial sulfur transfer systems., Nakai Y,Nakai M,Hayashi H, The Journal of biological chemistry, 41, Vol. 283, No. 41, p. 27469-27476, 2008/10/10
  • Chloroplast outer envelope protein CHUP1 is essential for chloroplast anchorage to the plasma membrane and chloroplast movement, Kazusato Oikawa,Akihiro Yamasato,Sam-Geun Kong,Masahiro Kasahara,Masato Nakai,Fumio Takahashi,Yasunobu Ogura,Takatoshi Kagawa,Masamitsu Wada, PLANT PHYSIOLOGY, AMER SOC PLANT BIOLOGISTS, Vol. 148, No. 2, p. 829-842, 2008/10
  • Structural analysis of Arabidopsis CnfU protein: An iron-sulfur cluster biosynthetic scaffold in chloroplasts, Toshiki Yabe,Eiki Yamashita,Akihiro Kikuchi,Kozo Morimoto,Atsushi Nakagawa,Tomitake Tsukihara,Masato Nakai, JOURNAL OF MOLECULAR BIOLOGY, ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD, Vol. 381, No. 1, p. 160-173, 2008/08
  • キーワード蛋白質の一生:葉緑体・Toc・Tic, 共立出版, 2008
  • Thio modification of yeast cytosolic tRNA is an iron-sulfur protein-dependent pathway, Yumi Nakai,Masato Nakai,Roland Lill,Tsutomu Suzuki,Hideyuki Hayashi, MOLECULAR AND CELLULAR BIOLOGY, AMER SOC MICROBIOLOGY, Vol. 27, No. 8, p. 2841-2847, 2007/04
  • Effects of Rubisco kinetics and Rubisco activation state on the temperature dependence of the photosynthetic rate in spinach leaves from contrasting growth temperatures, Wataru Yamori,Kensaku Suzuki,Ko Noguchi,Masato Nakai,Ichiro Terashima, Plant, Cell and Environment, Vol. 29, No. 8, p. 1659-1670, 2006/08
  • The asymmetric IscA homodimer with an exposed [2Fe-2S] cluster suggests the structural basis of the fe-S cluster biosynthetic scaffold, Kozo Morimoto,Eiki Yamashita,Youhei Kondou,Soo Jae Lee,Fumio Arisaka,Tomitake Tsukihara,Masato Nakai, JOURNAL OF MOLECULAR BIOLOGY, ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD, Vol. 360, No. 1, p. 117-132, 2006/06
  • Arabidopsis AtIscA-I is affected by deficiency of Fe-S cluster biosynthetic scaffold AtCnfU-V, T Yabe,M Nakai, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC ELSEVIER SCIENCE, Vol. 340, No. 4, p. 1047-1052, 2006/02
  • Characterization of the preprotein translocon at the outer envelope membrane of chloroplasts by blue native PAGE, Shingo Kikuchi,Toshiya Hirohashi,Masato Nakai, Plant and Cell Physiology, Vol. 47, No. 3, p. 363-371, 2006
  • The Arabidopsis chloroplastic NifU-Like protein CnfU can act as an iron-sulfur cluster scaffold protein and is required for biogenesis of ferredoxin and photosystem I, Toshiki Yabe,Kozo Morimoto,Shingo Kikuchi,Kazuaki Nishio,Ichiro Terashima,Masato Nakai, Photosynthesis: Fundamental Aspects to Global Perspectives (A. van der Est, and D. Bruce. eds.), Kluwer Academic Publishers, Dordrecht, Vol. pp.793-795, 2005
  • 細胞内タンパク質の社会学:解明されはじめた葉緑体タンパク質配送システムのユニークさ, 羊土社, 2005
  • Reinvestigation of the requirement of cytosolic ATP for mitochondrial protein import, T Asai,T Takahashi,M Esaki,S Nishikawa,K Ohtsuka,M Nakai,T Endo, JOURNAL OF BIOLOGICAL CHEMISTRY, AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, Vol. 279, No. 19, p. 19464-19470, 2004/05
  • Yeast Nfs1p is involved in thio-modification of both mitochondrial and cytoplasmic tRNAs, Y Nakai,N Umeda,T Suzuki,M Nakai,H Hayashi,K Watanabe,H Kagamiyama, JOURNAL OF BIOLOGICAL CHEMISTRY, AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, Vol. 279, No. 13, p. 12363-12368, 2004/03
  • Maize mutants lacking chloroplast FtsY exhibit pleiotropic defects in the biogenesis of thylakoid membranes, Y Asakura,T Hirohashi,S Kikuchi,S Belcher,E Osborne,S Yano,Terashima, I,A Barkan,M Nakai, PLANT CELL, AMER SOC PLANT BIOLOGISTS, Vol. 16, No. 1, p. 201-214, 2004/01
  • 蛋白質核酸酵素増刊「細胞における蛋白質の一生」葉緑体の蛋白質輸送:チラコイド, 共立出版, 2004
  • Protein targeting to thylakoids, Yukari Asakura,Shingo Kikuchi,Masato Nakai, KYORITSU SHUPPAN CO., LTD., Vol. 49, p. 922-925, 2004/01/01
  • A HEAT-repeats containing protein, IaiH, stabilizes the iron-sulfur cluster bound to the cyanobacterial IscA homologue, IscA2, K Morimoto,S Sato,S Tabata,M Nakai, JOURNAL OF BIOCHEMISTRY, JAPANESE BIOCHEMICAL SOC, Vol. 134, No. 2, p. 211-217, 2003/08
  • Deletion of a Chaperonin 60β gene leads to cell death in the Arabidopsis lesion initiation 1 mutant, Atsushi Ishikawa,Hideaki Tanaka,Masato Nakai,Tadashi Asahi, Plant and Cell Physiology, Vol. 44, No. 3, p. 255-261, 2003/03/01
  • Effect of photooxidative destruction of chloroplasts on the expression of nuclear genes for C4 photosynthesis and for chloroplast biogenesis in maize, Yosuke Tamada,Eiji Imanari,Ken-Ichi Kurotani,Masato Nakai,Carlos S. Andreo,Katsura Izui, Journal of Plant Physiology, Elsevier GmbH, Vol. 160, No. 1, p. 3-8, 2003/01/01
  • csr1, the maize mutants of chloroplast FtsY exhibit a pleicrtrophic defect in biogenesis of thylakoids., Asakura Yukari,Hirohashi Toshiya,Kikuchi Shingo,Nakai Masato,Belcher Susan,Osborne Erin,Barkan Alice,Yano Satoshi,Terashima Ichir, Plant Biology (Rockville), Vol. 2003, 2003
  • 鉄硫黄クラスターの生合成はどこまでわかったか?, 化学と生物(学会出版センター), Vol. 41, No. 10, p. 680-686, 2003
  • Identification of a novel prokaryotic HEAT-repeats-containing protein which interacts with a cyanobacterial IscA homolog, Kozo Morimoto,Kazuaki Nishio,Masato Nakai, FEBS Letters, Vol. 519, No. 1-3, p. 123-127, 2002/05/22
  • Molecular scaffolds involved in iron-sulfur cluster biosynthesis (Review), Masato Nakai,Kazuaki Nishio,Kozo Morimoto,Toshiki Yabe,Shingo Kikuchi, Recent Res. Devel. Proteins, Vol. 1, p. 1-11, 2002
  • Maize non-photosynthetic ferredoxin precursor is mis-sorted to the intermembrane space of chloroplasts in the presence of light, T Hirohashi,T Hase,M Nakai, PLANT PHYSIOLOGY, AMER SOC PLANT PHYSIOLOGISTS, Vol. 125, No. 4, p. 2154-2163, 2001/04
  • Nuclear localization of yeast Nfs1p is required for cell survival., Nakai Y,Nakai M,Hayashi H,Kagamiyama H, J Biol Chem., Vol. 276, No. 11, p. 8314-8320, 2001/03
  • Mitochondrial localization of eukaryotic NifS-like proteins, Y Nakai,Y Yoshihara,M Nakai,H Hayashi,H Kagamiyama, BIOCHEMISTRY AND MOLECULAR BIOLOGY OF VITAMIN B6 AND PQQ-DEPENDENT PROTEINS, BIRKHAUSER VERLAG AG, p. 129-133, 2000
  • Chloroplast chaperonins: Evidence for heterogeneous assembly of α and β Cpn60 polypeptides into a chaperonin oligomer, Kazuaki Nishio,Toshiya Hirohashi,Masato Nakai, Biochemical and Biophysical Research Communications, Academic Press Inc., Vol. 266, No. 2, p. 584-587, 1999/12/20
  • Involvement of a chloroplast homologue of the signal recognition particle receptor protein, FtsY, in protein targeting to thylakoids, Naoko Kogata,Kazuaki Nishio,Toshiya Hirohashi,Shingo Kikuchi,Masato Nakai, FEBS Letters, Vol. 447, No. 2-3, p. 329-333, 1999/03/26
  • Uncoupling of transfer of the presequence and unfolding of the mature domain in precursor translocation across the mitochondrial outer membrane, T Kanamori,S Nishikawa,M Nakai,Shin, I,PG Schultz,T Endo, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, NATL ACAD SCIENCES, Vol. 96, No. 7, p. 3634-3639, 1999/03
  • Cloning and characterization of chloroplast Sec-Y proteins., T Nohara,T Yoshihisa,M Nakai,T Endo, PHOTOSYNTHESIS: MECHANISMS AND EFFECTS, VOLS I-V, SPRINGER, p. 3115-3118, 1998
  • Cytochrome f encoded by the chloroplast genome is imported into thylakoids via the SecA-dependent pathway, T Nohara,T Asai,M Nakai,M Sugiura,T Endo, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS, Vol. 224, No. 2, p. 474-478, 1996/07
  • Erratum: Cytochrome f encoded by the chloroplast genome is imported into thylakoids via the SecA-dependent pathway (Biochemical and Biophysical Research Communications (1996) 224 No 2 (474-478)), T. Nohara,T. Asai,M. Nakai,M. Sugiura,T. Endo, Biochemical and Biophysical Research Communications, Vol. 227, 1996/01/01
  • Mitochondrial receptor complex protein - The intermembrane space domain of yeast MAS17 is not essential for its targeting or function, M Nakai,K Kinoshita,T Endo, JOURNAL OF BIOLOGICAL CHEMISTRY, AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, Vol. 270, No. 51, p. 30571-30575, 1995/12
  • ISOLATION AND CHARACTERIZATION OF THE CDNA FOR PEA CHLOROPLAST SECA EVOLUTIONARY CONSERVATION OF THE BACTERIAL-TYPE SECA-DEPENDENT PROTEIN-TRANSPORT WITHIN CHLOROPLASTS, T NOHARA,M NAKAI,A GOTO,T ENDO, FEBS LETTERS, ELSEVIER SCIENCE BV, Vol. 364, No. 3, p. 305-308, 1995/05
  • Molecular chaperone hsp70 and organelle biogenesis, M. Nakai,S. Nishikawa,T. Endo, Seikagaku. The Journal of Japanese Biochemical Society, Vol. 67, p. 108-122, 1995/02/01
  • Cloning and characterization of Sec and FFH genes from the cyanobacterium Synechococcus PCC7942, M Nakai,T Nohara,D Sugita,T Endo, PHOTOSYNTHESIS: FROM LIGHT TO BIOSPHERE, VOL III, KLUWER ACADEMIC PUBL, p. 505-508, 1995
  • Roles of Sec proteins in protein transport within chloroplasts., T Endo,T Nohara,A Goto,M Nakai, PHOTOSYNTHESIS: FROM LIGHT TO BIOSPHERE, VOL III, KLUWER ACADEMIC PUBL, p. 811-814, 1995
  • 分子シャペロンhsp70とオルガネラ形成, 生化学, Vol. 67, p. 108-122, 1995
  • Avidin fusion protein as a tool to generate a stable translocation intermediate spanning the mitochondrial membranes, Toshiya Endo,Yumiko Nakayama,Masato Nakai, Journal of Biochemistry, Oxford University Press, Vol. 118, No. 4, p. 753-759, 1995
  • IDENTIFICATION OF THE SECA PROTEIN HOMOLOG IN PEA-CHLOROPLASTS AND ITS POSSIBLE INVOLVEMENT IN THYLAKOIDAL PROTEIN-TRANSPORT, M NAKAI,A GOTO,T NOHARA,D SUGITA,T ENDO, JOURNAL OF BIOLOGICAL CHEMISTRY, AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, Vol. 269, No. 50, p. 31338-31341, 1994/12
  • IDENTIFICATION AND CHARACTERIZATION OF THE SECA PROTEIN HOMOLOG IN THE CYANOBACTERIUM SYNECHOCOCCUS PCC7942, M NAKAI,T NOHARA,D SUGITA,T ENDO, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS, Vol. 200, No. 2, p. 844-851, 1994/04
  • ACIDIC REGIONS OF CYTOCHROME C1 ARE ESSENTIAL FOR UBIQUINOL-CYTOCHROME-C-1 REDUCTASE-ACTIVITY IN YEAST-CELLS LACKING THE ACIDIC QCR6 PROTEIN, M NAKAI,T ENDO,T HASE,Y TANAKA,BL TRUMPOWER,H ISHIWATARI,A ASADA,M BOGAKI,H MATSUBARA, JOURNAL OF BIOCHEMISTRY, JAPANESE BIOCHEMICAL SOC, Vol. 114, No. 6, p. 919-925, 1993/12
  • INTRAMITOCHONDRIAL PROTEIN SORTING - ISOLATION AND CHARACTERIZATION OF THE YEAST MSP1 GENE WHICH BELONGS TO A NOVEL FAMILY OF PUTATIVE ATPASES, M NAKAI,T ENDO,T HASE,H MATSUBARA, JOURNAL OF BIOLOGICAL CHEMISTRY, AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, Vol. 268, No. 32, p. 24262-24269, 1993/11
  • THE BCL-2 PROTEIN IS INSERTED INTO THE OUTER-MEMBRANE BUT NOT INTO THE INNER MEMBRANE OF RAT-LIVER MITOCHONDRIA IN-VITRO, M NAKAI,A TAKEDA,ML CLEARY,T ENDO, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS, Vol. 196, No. 1, p. 233-239, 1993/10
  • CLONING OF THE YAP19 GENE ENCODING A PUTATIVE YEAST HOMOLOG OF AP19, THE MAMMALIAN SMALL CHAIN OF THE CLATHRIN-ASSEMBLY PROTEINS, M NAKAI,T TAKADA,T ENDO, BIOCHIMICA ET BIOPHYSICA ACTA, ELSEVIER SCIENCE BV, Vol. 1174, No. 3, p. 282-284, 1993/09
  • SEC-Y PROTEIN IS LOCALIZED IN BOTH THE CYTOPLASMIC AND THYLAKOID MEMBRANES IN THE CYANOBACTERIUM SYNECHOCOCCUS PCC7942, M NAKAI,D SUGITA,T OMATA,T ENDO, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS, Vol. 193, No. 1, p. 228-234, 1993/05
  • Acidic regions of cytochrome c1 are essential for ubiquinol-cytochrome c reductase activity in yeast cells lacking the acidic QCR6 protein, Masato Nakai,Toshiya Endo,Toshiharu Hase,Yoshikazu Tanaka,Bernard L. Trumpower,Haruko Ishiwatari,Akiko Asada,Mayumi Bogaki,Hiroshi Matsubara, Journal of Biochemistry, Oxford University Press, Vol. 114, No. 6, p. 919-925, 1993
  • CLONING AND CHARACTERIZATION OF THE SECY GENE FROM THE CYANOBACTERIUM SYNECHOCOCCUS PCC7942, M NAKAI,A TANAKA,T OMATA,T ENDO, BIOCHIMICA ET BIOPHYSICA ACTA, ELSEVIER SCIENCE BV, Vol. 1171, No. 1, p. 113-116, 1992/11
  • THE CHLOROPLAST-TARGETING DOMAIN OF PLASTOCYANIN TRANSIT PEPTIDE CAN FORM A HELICAL STRUCTURE BUT DOES NOT HAVE A HIGH-AFFINITY FOR LIPID BILAYERS, T ENDO,K KAWAMURA,M NAKAI, EUROPEAN JOURNAL OF BIOCHEMISTRY, SPRINGER VERLAG, Vol. 207, No. 2, p. 671-675, 1992/07
  • Methods for studying protein import into isolated mitochondria, T. Endo,M. Nakai, Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme, Vol. 36, No. 12, p. 1951-1956, 1991/10/01
  • ミトコンドリア膜透過実験法, 蛋白質・核酸・酵素, Vol. 36, No. 12, 1991
  • REPLACEMENT OF PUTATIVE AXIAL LIGANDS OF HEME IRON IN YEAST CYTOCHROME-C1 BY SITE-DIRECTED MUTAGENESIS, M NAKAI,H ISHIWATARI,A ASADA,M BOGAKI,K KAWAI,Y TANAKA,H MATSUBARA, JOURNAL OF BIOCHEMISTRY, JAPAN BIOCHEMICAL SOC, Vol. 108, No. 5, p. 798-803, 1990/11
  • Replacement of putative axial ligands of heme iron in yeast cytochrome c1 by site-directed mutagenesis, Masato Nakai,Haruko Ishiwatari,Akiko Asada,Mayumi Bogaki,Kiyozo Kawai,Yoshikazu Tanaka,Hiroshi Matsubara, Journal of Biochemistry, Oxford University Press, Vol. 108, No. 5, p. 798-803, 1990
  • PROTEIN SORTING BETWEEN THE OUTER AND INNER MITOCHONDRIAL-MEMBRANES - SUBMITOCHONDRIAL LOCALIZATION OF CYTOCHROME-C1 WHOSE PRESEQUENCE IS REPLACED BY THE AMINO-TERMINAL REGION OF A 70-KDA OUTER-MEMBRANE PROTEIN, M NAKAI,M HARABAYASHI,T HASE,H MATSUBARA, JOURNAL OF BIOCHEMISTRY, JAPANESE BIOCHEMICAL SOC, Vol. 106, No. 1, p. 181-187, 1989/07
  • Precise determination of the mitochondrial import signal contained in a 70 kDa protein of yeast mitochondrial outer membrane, Masato Nakai,Toshiharu Hase,Hiroshi Matsubara, Journal of Biochemistry, Oxford University Press, Vol. 105, No. 4, p. 513-519, 1989
  • ミトコンドリア外膜・内膜の局在化シグナル, 生体の科学, Vol. 39, No. 6, 1988
  • THE N-TERMINAL-21 AMINO-ACIDS OF A 70-KDA PROTEIN OF THE YEAST MITOCHONDRIAL OUTER-MEMBRANE DIRECT ESCHERICHIA-COLI BETA-GALACTOSIDASE INTO THE MITOCHONDRIAL MATRIX SPACE IN YEAST-CELLS, T HASE,M NAKAI,H MATSUBARA, FEBS LETTERS, ELSEVIER SCIENCE BV, Vol. 197, No. 1-2, p. 199-203, 1986/03

Misc.

  • Wobble U34 modification of plant cytosolic tRNAs and their possible effects on leaf morphogenesis, Yumi Nakai,Gorou Horiguchi,Kosei Iwabuchi,Kentaro Tamura,Akiko Harada,Masato Nakai,Ikuko Hara-Nishimura,Takato Yano, TAIWAN-Japan 2017 Plant Biology Conference, 2017/11
  • tRNAwobbleU34修飾欠損のシロイヌナズナ葉の形態形成への影響, 中井由実,堀口吾朗,岩渕功誠,田村謙太郎,原田明子,中井正人,西村いくこ,矢野貴人, 日本生化学会大会(Web), Vol. 90th, 2017
  • 植物tRNAのwobble位修飾とロゼッタ葉の形態形成について, 中井由実,堀口吾朗,原田明子,中井正人,矢野貴人, 日本分子生物学会年会プログラム・要旨集(Web), Vol. 39th, 2016
  • Ycf1: A green TIC: Response to the de Vries et al. Commentary, Masato Nakai, Plant Cell, American Society of Plant Biologists, Vol. 27, No. 7, p. 1834-1838, 2015/07/01
  • シロイヌナズナにおけるtRNA硫黄修飾の欠失は植物の乾燥ストレス条件下での成長に影響する, 中井由実,原田明子,中井正人,矢野貴人, 日本植物生理学会年会要旨集, Vol. 56th, 2015/03/09
  • 植物における含硫小分子への硫黄供給経路とその細胞内局在性, 中井由実,原田明子,橋口康之,中井正人,矢野貴人, 日本生化学会大会(Web), Vol. 88th, 2015
  • シロイヌナズナの環境ストレス下における生育とtRNA硫黄修飾の関連について, 中井由実,原田明子,中井正人,矢野貴人, 日本生化学会大会(Web), Vol. 87th, 2014
  • モリブドプテリン生合成中に含まれるArabidopsis硫黄トランスフェラーゼ蛋白質であるAtCnx5はtRNAのチオ修正にも必要である, 中井由実,原田明子,橋口康之,中井正人, 日本植物生理学会年会要旨集, 2013
  • Urm1とUba4は細胞内含硫小分子への硫黄運搬とUrmylationに関わるUBL-UBAシステムである, 中井 由実,原田 明子,橋口 康之,中井 正人,林 秀行, 日本生化学会大会プログラム・講演要旨集, (公社)日本生化学会, Vol. 85回, p. 3P-236, 2012/12
  • NAKAI Yumi、NAKAI Masato、HAYASHI Hideyuki, Yumi Nakai, 2011
  • シロイヌナズナおよび酵母においてサイトゾルのtRNA硫黄修飾に関与する,ユビキチン様(Ubl)タンパク質とユビキチン活性化酵素様(UBA)タンパク質の比較解析, 中井由実,原田明子,橋口康之,中井正人,林秀行, 生化学, 2011
  • 酵母Urm1‐Uba4は酵母サイトゾルtRNAの硫黄修飾に必要なタンパク質修飾系(UBL‐UBA)である。, 中井由実,中井正人,林秀行, 生化学, 2010
  • 酵母の鉄硫黄クラスター生合成とtRNA硫黄修飾の関連について, 中井由実,中井正人,林秀行, 生化学, 2009/09/25
  • ユビキチン様タンパク質Urm1と硫黄運搬タンパク質Uba4は酵母サイトゾルtRNAの硫黄修飾に必要である。, 中井由実,中井正人,林秀行, 生化学, 2008
  • Key words: Life of Proteins: Chloroplast; Toc; Tic, Kyoritsu Shuppan, 2008
  • Biochemical properties of HCF101 involved in iron-sulfur cluster biosynthesis in plant chloroplasts, Toshiki Yabe,Hiroshi Hori,Masato Nakai, PLANT AND CELL PHYSIOLOGY, OXFORD UNIV PRESS, Vol. 48, p. S127-S127, 2007
  • 酵母tRNAの2チオ修飾と鉄硫黄クラスター形成経路の関係, 中井由実,中井正人,ROLAND Lill,林秀行, 生化学, 2007
  • 酵母tRNALysUUUのwobble位2チオ修飾と鉄硫黄クラスター生合成との関連について, 中井由実,中井正人,LILL Roland,鈴木勉,林秀行, 日本RNA学会年会要旨集, 2006
  • Protein Sociology: Chloroplast protein import and intraorganellar protein trafficking, Yodosha, 2005
  • Photosynthesis: Fundamental Aspects to Global Perspectives (A. van der Est, D. Bruce, eds): The Arabidopsis chloroplastic NifU-like protein AtCnfU can act as an iron-sulfur cluster scaffold protein and is required for biogenesis of ferredoxin and photo・・・, Allen Press., 2005
  • Photosynthesis: Fundamental Aspects to Global Perspectives (A. van der Est, D. Bruce, eds), Characterization of the preprotein translocon at the outer envelople membrane of chloroplasts by Blue-Native Page., Allen Press., 2005
  • Photosynthesis: Fundamental Aspects to Global Perspectives (A. van der Est, D. Bruce, eds), Mechanism of temperature acclimation for photosynthesis in spinach leaves., Allen Press., 2005
  • 葉緑体の蛋白質輸送:チラコイド, 蛋白質核酸酵素5月号増刊「細胞における蛋白質の一生:生成・成熟・輸送・管理・分解・病態」(共立出版), Vol. 49, 922-925./,, 2004
  • 酵母IscSホモログ,yNfs1pの核移行シグナルは生育に必須である, 中井由実,中井正人,林秀行,鏡山博行, 生化学, Vol. 73, No. 8, 2001/08/25
  • 酵母yNfs1pの核移行シグナル様配列, 中井由実,中井正人,林秀行,鏡山博行, 生化学, Vol. 72, No. 8, 2000/08/25
  • 酵母ミトコンドリア内hsp70とそのパートナータンパク質のシャペロン機能の解析, 常広岳史,久保祐子,西川周一,中井正人,池田恵理,東江昭夫,森島信裕,柴田武彦,遠藤斗志也, 日本分子生物学会年会プログラム・講演要旨集, Vol. 21st, 1998/11
  • Characterization of cytosolic factors in rabbit reticulocyte lysate and yeast cell lysate mediating mitochondrial protein import., 浅井健好,常広岳史,西川周一,中井正人,遠藤斗志也, 日本分子生物学会年会プログラム・講演要旨集, Vol. 20th, 1997/12
  • Cytochrome f encoded by the chloroplast genome is imported into thylakoids via the SecA-dependent pathway (vol 224, pg 474, 1996), T Nohara,T Asai,M Nakai,M Sugiura,T Endo, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS, Vol. 227, No. 2, p. 644-644, 1996/10
  • The comparison of yeast cytosol and chaperon function of hsp70 of the mitochondrion matrix., 久保祐子,西川周一,中井正人,三井里子,森島信裕,柴田武彦,遠藤斗志也, 日本分子生物学会年会プログラム・講演要旨集, Vol. 19th, 1996/07
  • Identification of yeast MAS17 encoding the functional counterpart of the mitochondrial receptor complex protein MOM22 of Neurospora crassa, Masato Nakai,Toshiya Endo, FEBS Letters, Vol. 357, No. 2, p. 202-206, 1995/01/03
  • Large-scale preparation and functional analyses of yeast mitochondrial import receptors, MAS70 and MAS20., 鳥居久義,西川周一,中井正人,遠藤斗志也, 日本分子生物学会年会プログラム・講演要旨集, Vol. 17th, 1994/11
  • In vitro assembly of mitochondrion-encoded proteins into the mitochondrial inner membrane., 山本佳奈,加藤祐治,西川周一,中井正人,遠藤斗志也, 日本分子生物学会年会プログラム・講演要旨集, Vol. 17th, 1994/11
  • ACIDIC REGIONS OF CYTOCHROME C1 ARE ESSENTIAL FOR UBIQUINOL-CYTOCHROME-C-1 REDUCTASE-ACTIVITY IN YEAST-CELLS LACKING THE ACIDIC QCR6 PROTEIN, M NAKAI,T ENDO,T HASE,Y TANAKA,BL TRUMPOWER,H ISHIWATARI,A ASADA,M BOGAKI,H MATSUBARA, JOURNAL OF BIOCHEMISTRY, JAPANESE BIOCHEMICAL SOC, Vol. 114, No. 6, p. 919-925, 1993/12
  • INTRAMITOCHONDRIAL PROTEIN SORTING - ISOLATION AND CHARACTERIZATION OF THE YEAST MSP1 GENE WHICH BELONGS TO A NOVEL FAMILY OF PUTATIVE ATPASES, M NAKAI,T ENDO,T HASE,H MATSUBARA, JOURNAL OF BIOLOGICAL CHEMISTRY, AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, Vol. 268, No. 32, p. 24262-24269, 1993/11
  • REPLACEMENT OF PUTATIVE AXIAL LIGANDS OF HEME IRON IN YEAST CYTOCHROME-C1 BY SITE-DIRECTED MUTAGENESIS, M NAKAI,H ISHIWATARI,A ASADA,M BOGAKI,K KAWAI,Y TANAKA,H MATSUBARA, JOURNAL OF BIOCHEMISTRY, JAPAN BIOCHEMICAL SOC, Vol. 108, No. 5, p. 798-803, 1990/11
  • PROTEIN SORTING BETWEEN THE OUTER AND INNER MITOCHONDRIAL-MEMBRANES - SUBMITOCHONDRIAL LOCALIZATION OF CYTOCHROME-C1 WHOSE PRESEQUENCE IS REPLACED BY THE AMINO-TERMINAL REGION OF A 70-KDA OUTER-MEMBRANE PROTEIN, M NAKAI,M HARABAYASHI,T HASE,H MATSUBARA, JOURNAL OF BIOCHEMISTRY, JAPANESE BIOCHEMICAL SOC, Vol. 106, No. 1, p. 181-187, 1989/07
  • Signals guiding Proteins to mitochondrial membranes, Vol. 39, No. 6, 1988

Publications

  • 葉緑体のタンパク質輸送機構についてーシアノバクテリアの内共生から始まったユニークな進化, 生物の科学:遺伝 3月号, 2016
  • 明らかになった葉緑体のトランスロコン, 細胞工学8月号(学研メディカル秀潤社), 2013
  • One- and Two-Dimensional Blue Native-PAGE and Immunodetection of Low-Abundance Chloroplast Membrane Protein Complexes. In Chloroplast Research in Arabidopsis : Methods and Protocols. Methods in Molecular Biology, 775:3-17, 2011., Shingo Kikuchi,Jocelyn Bedard,Masato Nakai, Humana Press, 2011/09
  • One- and Two-Dimensional Blue Native-PAGE and Immunodetection of Low-Abundance Chloroplast Membrane Protein Complexes. In Chloroplast Research in Arabidopsis : Methods and Protocols. Methods in Molecular Biology, 775:3-17, 2011., Shingo Kikuchi,Jocelyn Bedard,Masato Nakai, Humana Press, ISBN:9781617792366, 2011/09
  • キーワード蛋白質の一生:葉緑体・Toc・Tic, 共立出版, 2008
  • 細胞内タンパク質の社会学:解明されはじめた葉緑体タンパク質配送システムのユニークさ, 羊土社, 2005
  • 蛋白質核酸酵素増刊「細胞における蛋白質の一生」葉緑体の蛋白質輸送:チラコイド, 共立出版, 2004
  • 葉緑体の蛋白質輸送:チラコイド, 蛋白質核酸酵素5月号増刊「細胞における蛋白質の一生:生成・成熟・輸送・管理・分解・病態」(共立出版), 2004
  • 鉄硫黄クラスターの生合成はどこまでわかったか?, 化学と生物(学会出版センター), 2003
  • 葉緑体へのタンパク質輸送はどこまでわかったか-共通性と独自性, 実験医学別冊「細胞内の輸送研究の最前線」(羊土社), 2003
  • 葉緑体への輸送, わかる実験医学シリーズ「細胞内輸送がわかる」(羊土社), 2002
  • Molecular scaffolds involved in iron-sulfun cluster Biosynthesis, Recent Research Development in Proteins, 2002
  • 葉緑体分子シャペロン, 分子シャペロンによる細胞機能制御, 2001
  • ┣DBMitochondrial Localization of Eukalyctic Nifs-likeProteins(/)-┫DB, ┣DBBiochemistry and Molecular Biology of Vitamin B6 and PQQ-Dependent Proteins.(/)-┫DB, 2000
  • Fe-S cluster formation of ferredoxin in chloraplast stroma, Research in Photosynthesis, 1999
  • Cloning and characterization of chloroplast SecY proteins, Research in Photosynthesis, 1999
  • 葉緑体ストロマからの分子シャペロンの精製, 植物のタンパク質実験プロトコール-植物細胞工学別刷, 1998
  • タンパク質の抽出法 1-4.細胞の破砕と分画-酵母, タンパク質実験ノート(羊土社), 1996
  • 分子シャペロンhsp70とオルガネラ形成, 生化学, 1995
  • Roles of Sec proteins in protein transport within chloroplasts, Research in Photosynthesis : from Light to biosphere, 1995
  • Cloning and characterization of sec and ffh genes from the cyanobacterium Synechococcus PCC7942, Research in Photosynthesis : from Light to biosphere, 1995
  • Conformation of the chloroplast-targeting domain of plastocyanin transit peptide and its interaction with lipid bilayers, Research in Photosynthesis, 1992
  • ミトコンドリア膜透過実験法, 蛋白質・核酸・酵素, 1991
  • ミトコンドリア外膜・内膜の局在化シグナル, 生体の科学, 1988

Awards

  • Rebeiz Foundation for Basic Research Annual Paper Award, NAKAI Masato, The Rebeiz Foundation, USA, 2013/11
  • 井上学術奨励賞, 1990

Presentations

  • Molecular machineries for chloroplast protein import and their evolution., Masato Nakai, INTERNATIONAL SYMPOSIUM ON PHOTOSYNTHESIS AND CHLOROPLAST BIOGENESIS, 2018/11
  • Molecular machineries for chloroplast protein import and their evolution. (Plenary Lecture), Masato Nakai, 1st Asia-Oceania International Congress on Photosynthesis?, 2018/08
  • Molecular machineries for chloroplast protein import and their evolution, Masato Nakai, PSC Frontier Seminar Series, 2018/08
  • molecular mechanisms of chloroplast protein import and their curious evolutionary history, Masato Nakai, Taiwan-Japan Plant Biology 2017, 2017/11
  • molecular mechanisms of chloroplast protein import and their curious evolutionary history, KAAB International Symposium 2017: Frontiers in Plant Science and Biotechnology, 2017/09
  • molecular mechanisms of chloroplast protein import and their curious evolutionary history, Chloroplast Metabolism and Photosynthesis, 2017/06
  • Chloroplast protein import motor has evolved from a membrane-bound AAA protease., 第39回 日本分子生物学会シンポジウム “ほどく、ひきぬく、歩く:AAA+ ATPaseの作動原理”, 2016/12
  • Chloroplast protein import: Mechanisms and evolution., International Meeting 2016: Nascent Chain Biology, 2016/09
  • The chloroplast protein import system: Evolution and mechanisms., Gordon Research Conference “Mitochondria & Chloroplast: Evolution, Biogenesis and Quality Control of Organelles of Endosymbiotic Origin”, 2016/06
  • New insights into the molecular mechanisms and the molecular evolution of chloroplast protein import, BMB2015 日本生化学会・日本分子生物学会合同大会シンポジウム “オルガネラーバイオロジー:細胞の構造と機能の新しい姿”, 2015/12
  • The evolution of the chloroplast protein import system, International Meeting of “Matryoshka-type Evolution”, 2015/09
  • The evolution of the chloroplast protein import system, CIFAR (Canadian Institute for Advanced Research): Integrated Microbial Biodiversity Program Meeting, 2015/05
  • The Evolution of the Chloroplast Protein Import System, Tokyo Tech-HHU Dusseldorf Joint Symposium on Photosynthesis as a New Chemical Resource, 2015/03
  • Unraveling the Mechanism of Protein Transport Across the Chloroplast Inner Envelope, EMBO Conference on Mechanisms and regulation of protein translocation, 2015/03
  • Unraveling the Mechanism of Protein Transport Across the Chloroplast Inner Envelope Membrane., 日本学術振興会 日本・フィンランド二国間交流セミナー, 2014/10
  • Unraveling the Mechanism of Protein Transport Across the Chloroplast Inner Envelope Membrane., The International Symposium on the Regulation of Photosynthetic Function, 2014/08
  • Unraveling the mechanism of protein transport across the chloroplast inner envelope membrane, East Asian Cell Biology Conference, 2014/04
  • Reevaluation of the involvement of the "old" Tic proteins in chloroplast protein import, The 16th Annual Meeting of The French Society of Photosynthesis, 2014/04
  • The evolution of the chloroplast protein import system, A DYNAMO LABEX SYMPOSIUM "Evolution, biogenesis and dynamics of energy transducing membranes", 2014/04
  • 葉緑体タンパク質輸送装置の確立・進化, 第36回 日本分子生物学会年会 ワークショップ "寄生・共生におけるゾンビ化機構の分子生物学的解析", 2013/12
  • 葉緑体タンパク質輸送と光合成超分子複合体の形成, 第77回 日本植物学会年会 シンポジウム "光合成の超分子複合体の構造と動態", 2013/09
  • New evolutionary insights into the chloroplast protein import system, The 12th International Forum on Infection and Immunity, 2013/09
  • A novel TIC translocon-associated import motor?, EMBO Conference "From Structure to Function of Translocation Machines", 2013/04
  • 葉緑体内包膜の新奇な蛋白質輸送装置:葉緑体進化の新たなる謎, 第15回 植物オルガネラワークショップ "第54回 日本植物生理学会 関連集会:オルガネラの進化とダイナミズム", 2013/03
  • 一次共生進化の初期過程でのタンパク質輸送装置の確立・進化, 第76回 日本植物学会年会 シンポジウム "マトリョーシカ型進化と植物:その多様性から分子基盤まで", 2012/09
  • Uncovering the protein translocon at the chloroplast inner envelope, Gordon Research Conference: "Mitochondria & Chloroplasts", 2012/08
  • Uncovering the protein translocon at the chloroplast inner envelope, 第12回 日本蛋白質科学会年会 シンポジウム, 2012/06
  • A novel TIC complex: Translocon at the inner envelope of chloroplasts, Gordon Research Conference:PROTEIN TRANSPORT ACROSS CELL MEMBRANES, 2012/03
  • A novel TIC complex: Translocon at the inner envelope of chloroplasts, EMBO Conference series "PROTEIN TRANSPORT SYSTEMS"- Structures, mechanisms, and medical aspects -, 2011/04
  • Chloroplast protein import and biogenesis, International GCOE Symposium, Picobiology: Life Science at the Atomic Level "Morphogenesis and Signaling - From Proteins, Organelles to Organisms -, 2010/11
  • A Novel TIC complex, translocon at the inner envelope of chloroplasts, The 3rd International Symposium on Protein Community, (ISPC-Nara 2010), 2010/09
  • New perspectives on the Tic complex, a translocon at the inner envelope of chloroplasts, 第82回 日本生化学会大会シンポジウム "蛋白質膜透過装置の構造とダイナミックな機能", 2009/10
  • Molecular Mechanisms of Protein import into chloroplasts., 2009 Plant Winter Conference, 2009/01
  • Protein import into chloroplasts is mediated by a 1-MD translocation complex at the inner envelope m, The EMBO Conference series "Control, co-ordination and regulation of protein targeting and translocation", 2008/10
  • CnfU, Nfu, & NifU : Similar but Distinct Fe-S Cluster Biosynthetic Scaffolds Required under Differen, Biogenesis of Iron Sulfur proteins: Cluster Assembly and regulation, 2007/07
  • Identification and characterization of a novel preprotein translocation intermediate at the inner en, Gordon Research Conference: Protein Transport Across Cell Membranes, 2007/06
  • Protein Transport Across the Chloroplast Envelope., EMBO Conference Series: Protein Transport System, 2006/10
  • CnfU & Nfu: Homologous Fe-S Cluster Biosynthetic Scaffolds, Gordon Research Conference: Mitochondria and Chloroplast, 2006/08
  • Protein Transport Across the Chloroplast Envelope Analyzed by Blue-Native PAGE., International Conference on Life of Proteins, 2005/11
  • 葉緑体タンパク質の生合成:タンパク質輸送とコファクターのアセンブリー, 第15回 関西光合成研究会, 2005/11
  • Protein Transport Across the Chloroplast Envelope., 2005 Swiss-Japan Chloroplast Workshop, 2005/10
  • 葉緑体タンパク質の生合成の視点から見る光合成研究の今後の展開., 北海道大学低温研究所研究セミナ−, 2005/06
  • The First Fe-S Cluster-Bound Crystal Structure of a Scaffold Protein., 31th Steenbock Symposium "Fe-S Proteins: Biogenesis, Strcuture and Function", 2005/05
  • 活性中心の生合成:鉄硫黄クラスターはどこからやってくる?, 第5回 日本光合成研究会シンポジウム「光合成研究入門:地球の未来を語ろう!」, 2005/05
  • 葉緑体への蛋白質輸送., 第27回 日本分子生物学会年会ワークショップ「タンパク質機能化の細胞内インフラストラクチャー」, 2004/12
  • 蛋白質膜透過装置のアーキテクチャーと膜透過中間体., 2004年度 日本植物学会シンポジウム 「パーツ(部品)の構築から見た葉緑体形成」, 2004/09
  • Molecular Scaffolds Involved in Iron-Sulfur Cluster Biosynthesis., 1st International Meeting on Iron-Sulfur Proteins, 2002/09
  • プラスチドの形成と制御., 2002年度日本細胞生物学会シンポジウム「オルガネラの形成と制御」, 2002/05
  • 葉緑体は光環境の変化に応じて蛋白質を選択的に取り込んでいるか?, 第4回植物オルガネラワークショップ「オルガネラ機能の制御系と進化」, 2002/03
  • プラスチドにおけるプロティンフラックス, 蛋白研セミナー「プラスチドのバイオジェネシス」, 2001/10
  • Light- and Redox-Regulated Protein Import into Chloroplasts., European Science Foundation Conference "Protein Targeting", 2001/09
  • 葉緑体チラコイド膜へのシグナル認識粒子依存性蛋白質輸送経路の解析, 蛋白研セミナー「膜蛋白質の生合成と蛋白質膜透過のメカニズム」, 1999/07
  • 葉緑体の分子シャペロン, 公開シンポジウム「分子シャペロン」, 1997/11

Committee Memberships

  • 植物生理学会, 広報幹事, 2003 - 2004