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Kiyozumi Daiji

淨住 大慈

Assistant Professor

keyword multicellular system,lumicrine,genome editing,reproductive biology,Experimental animals,Genetics,Developmental Biology,Organogenesis,Epithelial-mesencymal interactions,Fraser syndrome,cell adhesion,extracellular matrix

Research History

  • 2021/10 - Present, Japan Science and Technology Agency

Research Areas

  • Life sciences, Functional biochemistry
  • Life sciences, Genomics
  • Life sciences, Molecular biology
  • Life sciences, Animals: biochemistry, physiology, behavioral science
  • Life sciences, Zoological sciences
  • Life sciences, Urology
  • Life sciences, Cell biology
  • Life sciences, Laboratory animal science
  • Life sciences, Morphology, anatomy
  • Life sciences, Developmental biology

Professional Memberships

  • 日本分子生物学会
  • JAPANESE ASSOCIATION FOR LABORATORY ANIMAL SCIENCE
  • The Japanese Society for Connective TIssue Research
  • JAPANESE SOCIETY OF DEVELOPMENTAL BIOLOGISTS

Papers

  • NELL2-mediated lumicrine signaling through OVCH2 is required for male fertility, Daiji Kiyozumi,Taichi Noda,Ryo Yamaguchi,Tomohiro Tobita,Takafumi Matsumura,Kentaro Shimada,Mayo Kodani,Takashi Kohda,Yoshitaka Fujihara,Manabu Ozawa,Zhifeng Yu,Gabriella Miklossy,Kurt M. Bohren,Masato Horie,Masaru Okabe,Martin M. Matzuk,Masahito Ikawa, Science, Vol. 368, No. 6495, p. 1132-1135, 2020/06/05
  • Laminin is the ECM niche for trophoblast stem cells, Daiji Kiyozumi,Itsuko Nakano,Ryoko Sato-Nishiuchi,Satoshi Tanaka,Kiyotoshi Sekiguchi, Life Science Alliance, Vol. 3, No. 2, 2020
  • Laminin γ1 c-terminal Glu to Gln mutation induces early postimplantation lethality, Daiji Kiyozumi,Yukimasa Taniguchi,Itsuko Nakano,Junko Toga,Emiko Yagi,Hidetoshi Hasuwa,Masahito Ikawa,Kiyotoshi Sekiguchi, Life Science Alliance, Vol. 1, No. 5, 2018
  • Basement membrane assembly of the integrin α8β1 ligand nephronectin requires Fraser syndrome-associated proteins, Daiji Kiyozumi,Makiko Takeichi,Itsuko Nakano,Yuya Sato,Tomohiko Fukuda,Kiyotoshi Sekiguchi, Journal of Cell Biology, ROCKEFELLER UNIV PRESS, Vol. 197, No. 5, p. 677-689, 2012/05
  • Breakdown of the reciprocal stabilization of QBRICK/Frem1, Fras1, and Frem2 at the basement membrane provokes Fraser syndrome-like defects, Daiji Kiyozumi,Nagisa Sugimoto,Kiyotoshi Sekiguchi, Proceedings of the National Academy of Sciences of the United States of America, NATL ACAD SCIENCES, Vol. 103, No. 32, p. 11981-11986, 2006/08/08
  • Human disease-associated extracellular matrix orthologs ECM3 and QBRICK regulate primary mesenchymal cell migration in sea urchin embryos, Daiji Kiyozumi,Shunsuke Yaguchi,Junko Yaguchi,Atsuko Yamazaki,Kiyotoshi Sekiguchi, Experimental Animals, Japanese Association for Laboratory Animal Science, Vol. 70, No. 3, p. 378-386, 2021
  • CRISPR/Cas9-Mediated Genome Editing Reveals Oosp Family Genes are Dispensable for Female Fertility in Mice, Ferheen Abbasi,Mayo Kodani,Chihiro Emori,Daiji Kiyozumi,Masashi Mori,Yoshitaka Fujihara,Masahito Ikawa, Cells, Vol. 9, No. 4, 2020/03/28
  • Genetic mutation of Frem3 does not cause fraser syndrome in mice, Daiji Kiyozumi,Masashi Mori,Mayo Kodani,Masahito Ikawa, Experimental Animals, Vol. 69, No. 1, p. 104-109, 2020
  • CRISPR/Cas9-mediated genome editing reveals 30 testis-enriched genes dispensable for male fertility in mice, Yonggang Lu,Seiya Oura,Takafumi Matsumura,Asami Oji,Nobuyuki Sakurai,Yoshitaka Fujihara,Keisuke Shimada,Haruhiko Miyata,Tomohiro Tobita,Taichi Noda,Julio M. Castaneda,Daiji Kiyozumi,Qian Zhang,Tamara Larasati,Samantha A.M. Young,Mayo Kodani,Caitlin A. Huddleston,Matthew J. Robertson,Cristian Coarfa,Ayako Isotani,R. John Aitken,Masaru Okabe,Martin M. Matzuk,Thomas X. Garcia,Masahito Ikawa, Biology of Reproduction, Vol. 101, No. 2, p. 501-511, 2019/08/01
  • Ventricular–subventricular zone fractones are speckled basement membranes that function as a neural stem cell niche, Yuya Sato,Daiji Kiyozumi,Sugiko Futaki,Itsuko Nakano,Chisei Shimono,Naoko Kaneko,Masahito Ikawa,Masaru Okabe,Kazunobu Sawamoto,Kiyotoshi Sekiguchi, Molecular Biology of the Cell, Vol. 30, No. 1, p. 56-68, 2019/01/01
  • Lvrn expression is not critical for mouse placentation, Tomohiro Tobita,Daiji Kiyozumi,Masanaga Muto,Taichi Noda,Masahito Ikawa, Journal of Reproduction and Development, Vol. 65, No. 3, p. 239-244, 2019
  • Placenta-specific gene manipulation using lentiviral vector and its application, Tomohiro Tobita,Daiji Kiyozumi,Masahito Ikawa, Placenta, W B SAUNDERS CO LTD, Vol. 59, p. S37-S43, 2017/11
  • Genome engineering uncovers 54 evolutionarily conserved and testis-enriched genes that are not required for male fertility in mice, Haruhiko Miyata,Julio M. Castaneda,Yoshitaka Fujihara,Zhifeng Yu,Denise R. Archambeault,Ayako Isotani,Daiji Kiyozumi,Maya L. Kriseman,Daisuke Mashiko,Takafumi Matsumura,Ryan M. Matzuk,Masashi Mori,Taichi Noda,Asami Oji,Masaru Okabe,Renata Prunskaite-Hyyrylainen,Ramiro Ramirez-Solis,Yuhkoh Satouh,Qian Zhang,Masahito Ikawa,Martin M. Matzuk, Proceedings of the National Academy of Sciences of the United States of America, NATL ACAD SCIENCES, Vol. 113, No. 28, p. 7704-7710, 2016/07/12
  • Functional analysis of Plac1 in placental formation using knockout mice and lentiviral vector-mediated complementation, Masanaga Muto,Yoshitaka Fujihara,Tomohiro Tobita,Daiji Kiyozumi,Masahito Ikawa, 2016/02
  • Lentiviral vector-mediated complementation restored fetal viability but not placental hyperplasia in Plac1-deficient mice, Masanaga Muto,Yoshitaka Fujihara,Tomohiro Tobita,Daiji Kiyozumi,Masahito Ikawa, Biology of Reproduction, SOC STUDY REPRODUCTION, Vol. 94, No. 1, 2016/01/01
  • In situ detection of integrin ligands, Daiji Kiyozumi,Ryoko Sato-Nishiuchi,Kiyotoshi Sekiguchi, Current Protocols in Cell Biology, John Wiley and Sons Inc., Vol. 2014, p. 9.7.1-9.7.17, 2014
  • In situ detection of integrin ligands, Daiji Kiyozumi,Ryoko Sato-Nishiuchi,Kiyotoshi Sekiguchi, Current protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.], Vol. 65, p. 10.19.1-10.19.17, 2014
  • GPR56 Functions Together with α3β1 Integrin in Regulating Cerebral Cortical Development, Sung Jin Jeong,Rong Luo,Kathleen Singer,Stefanie Giera,Jordan Kreidberg,Daiji Kiyozumi,Chisei Shimono,Kiyotoshi Sekiguchi,Xianhua Piao, PLoS ONE, 7, Vol. 8, No. 7, 2013/07/09
  • Polydom/SVEP1 is a ligand for integrin α9β1, Ryoko Sato-Nishiuchi,Itsuko Nakano,Akio Ozawa,Yuya Sato,Makiko Takeichi,Daiji Kiyozumi,Kiyoshi Yamazaki,Teruo Yasunaga,Sugiko Futaki,Kiyotoshi Sekiguchi, Journal of Biological Chemistry, AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC, Vol. 287, No. 30, p. 25615-25630, 2012/07/20
  • Heterozygous mutations of FREM1 are associated with an increased risk of isolated metopic craniosynostosis in humans and mice, Lisenka E.L.M. Vissers,Timothy C. Cox,A. Murat Maga,Kieran M. Short,Fenny Wiradjaja,Irene M. Janssen,Fernanda Jehee,Debora Bertola,Jia Liu,Garima Yagnik,Kiyotoshi Sekiguchi,Daiji Kiyozumi,Hans van Bokhoven,Carlo Marcelis,Michael L. Cunningham,Peter J. Anderson,Simeon A. Boyadjiev,Maria Rita Passos-Bueno,Joris A. Veltman,Ian Smyth,Michael F. Buckley,Tony Roscioli, PLoS Genetics, PUBLIC LIBRARY SCIENCE, Vol. 7, No. 9, 2011/09
  • Fused pulmonary lobes is a rat model of human Fraser syndrome, Daiji Kiyozumi,Itsuko Nakano,Ken L. Takahashi,Hitoshi Hojo,Hiroaki Aoyama,Kiyotoshi Sekiguchi, Biochemical and Biophysical Research Communications, ACADEMIC PRESS INC ELSEVIER SCIENCE, Vol. 411, No. 2, p. 440-444, 2011/07/29
  • Identification of genes expressed during hair follicle induction, Daiji Kiyozumi,Aki Osada,Nagisa Sugimoto,Charles N. Weber,Yuichi Ono,Toshio Imai,Akiko Okada,Kiyotoshi Sekiguchi, Journal of Dermatology, WILEY-BLACKWELL, Vol. 38, No. 7, p. 674-679, 2011/07
  • Genetic analysis of fin development in zebrafish identifies furin and Hemicentin1 as potential novel fraser syndrome disease genes, Thomas J. Carney,Natália Martins Feitosa,Carmen Sonntag,Krasimir Slanchev,Johannes Kluger,Daiji Kiyozumi,Jan M. Gebauer,Jared Coffin Talbot,Charles B. Kimmel,Kiyotoshi Sekiguchi,Raimund Wagener,Heinz Schwarz,Phillip W. Ingham,Matthias Hammerschmidt, PLoS Genetics, PUBLIC LIBRARY SCIENCE, Vol. 6, No. 4, 2010/04
  • Transcriptome-based systematic identification of extracellular matrix proteins, Ri Ichiroh Manabe,Ko Tsutsui,Tomiko Yamada,Mina Kimura,Itsuko Nakano,Chisei Shimono,Noriko Sanzen,Yutaka Furutani,Tomohiko Fukuda,Yasuko Oguri,Keiko Shimamoto,Daiji Kiyozumi,Yuya Sato,Yoshikazu Sado,Haruki Senoo,Shohei Yamashina,Shiro Fukuda,Jun Kawai,Nobuo Sugiura,Koji Kimata,Yoshihide Hayashizaki,Kiyotoshi Sekiguchi, Proceedings of the National Academy of Sciences of the United States of America, NATL ACAD SCIENCES, Vol. 105, No. 35, p. 12849-12854, 2008/09/02
  • Frem3, a member of the 12 CSPG repeats-containing extracellular matrix protein family, is a basement membrane protein with tissue distribution patterns distinct from those of Fras1, Frem2, and QBRICK/Frem1, Daiji Kiyozumi,Nagisa Sugimoto,Itsuko Nakano,Kiyotoshi Sekiguchi, Matrix Biology, ELSEVIER SCIENCE BV, Vol. 26, No. 6, p. 456-462, 2007/07
  • Identification of a novel cell-adhesive protein spatiotemporally expressed in the basement membrane of mouse developing hair follicle, Daiji Kiyozumi,Aki Osada,Nagisa Sugimoto,Charles N. Weber,Yuichi Ono,Toshio Imai,Akiko Okada,Kiyotoshi Sekiguchi, Experimental Cell Research, ELSEVIER INC, Vol. 306, No. 1, p. 9-23, 2005/05/15
  • Expression of MAEG, a novel basement membrane protein, in mouse hair follicle morphogenesis, Aki Osada,Daiji Kiyozumi,Ko Tsutsui,Yuichi Ono,Charles N. Weber,Nagisa Sugimoto,Toshio Imai,Akiko Okada,Kiyotoshi Sekiguchi, Experimental Cell Research, ELSEVIER INC, Vol. 303, No. 1, p. 148-159, 2005/02/01
  • Pollen UDP-glucose pyrophosphorylase showing polymorphism well-correlated to the S genotype of Pyrus pyrifolia, Daiji Kiyozumi,Takeshi Ishimizu,Tetsu Nakanishi,Shigemi Norioka, Sexual Plant Reproduction, SPRINGER, Vol. 14, No. 6, p. 315-323, 2002

Misc.

  • 精子が「一人前」になる仕組み – 「ルミクライン因子」が司る精子成熟の分子メカニズムに迫る, 淨住大慈,伊川正人, academist Journal, 2020/10
  • 精子が受精能をもつための鍵分子を発見!, 淨住大慈, 化学, Vol. 75, p. 75-75, 2020/08
  • 新規なシグナル伝達システム「ルミクライン」による雄性生殖路の機能と妊孕性の制御, 淨住大慈,伊川正人, 実験医学, Vol. 38, No. 18, p. 3132-3135, 2020
  • 基底膜のカスタマイゼーションとその器官形成における役割, 浄住大慈,関口清俊, THE LUNG perspective, Vol. 15, No. 3, p. 336-340, 2007/06
  • Reciprocal stabilization of Fraser syndrome-associated proteins, D. Kiyozumi,N. Sugimoto,K. Sekiguchi, MATRIX BIOLOGY, ELSEVIER SCIENCE BV, Vol. 25, p. S81-S81, 2006/11
  • Extracellular matrix as a customied extracellular environment, Kiyozumi, D,Sekiguchi, K, Experimental Medicine, Vol. 23, No. 1, p. 52-57, 2005/01

Awards

  • Early Career Investigator Award, Daiji Kiyozumi, The 15th International Symposium of the Institute Network for Biomedical Sciences, 2020/11
  • 最優秀学術賞, 淨住 大慈, 大阪大学微生物病研究所 令和元年度研究業績発表会, 2020/01
  • Poster Award, Daiji Kiyozumi, Asia Pacific Developmental Biology Conference, 2012/10
  • The 10th IPR Retreat Best Oral Presentation Prize, Daiji Kiyozumi, The 10th IPR Retreat, 2011/11

Presentations

  • 雄性生殖路の細胞外環境と精子受精能, 淨住大慈, 新学術領域研究「配偶子インテグリティ」第3回 若手・公募班研究集会, 2021/07/06
  • 男性不妊の生物学:最新のゲノム編集技術で見えてきたこと, 淨住大慈, Science Cafe in Japan, 2021/06/29
  • 精巣上体における精子成熟とその分子基盤:管腔内環境の重要性, 淨住 大慈, 『配偶子インテグリティの構築』『全能性プログラム』合同公開シンポジウム2020, 2020/12
  • NELL2 is necessary for epididymal differentiation and male fertility, 淨住 大慈, 第15回生命医科学研究所ネットワーク国際シンポジウム, 2020/11
  • NELL2による生殖路を通じたルミクラインシグナル伝達は雄の妊孕性に必須である, 淨住大慈、野田大地、山口亮、飛田智央、松村貴史、島田健太郎、小谷まよ、幸田尚、藤原祥高、小沢学、Zhifeng Yu、Gabriella Miklossy、 Kurt M. Bohren、堀江正人、岡部勝、Martin M. Matzuk、伊川正人, 第52回日本結合組織学会学術大会, 2020/09
  • The Physiology of Laminins: Based on its Biochemistry, Kiyozumi D, IPR retreat 2011, 2011
  • Reciprocal stabilization of Fraser syndrome-associated proteins, Kiyozumi D,Sugimoto N,Sekiguchi K, The 3rd National Meeting of the American Society for Matrix Biology, 2006
  • 新規基底膜蛋白質QBRICKの同定とその基底膜局在機構, 淨住大慈,関口清俊, 大阪大学蛋白質研究所セミナー「基底膜研究の新展開」, 2006
  • Novel basement membrane proteins spatiotemporally expressed during hair follicle development, Kiyozumi D,Osada A,Sugimoto N,Weber C.N,Ono Y,Shimono C,Manabe R,Okada A,Sekiguchi K, Gordon Research Conference on Basement Membranes, 2004

Media Coverage

  • 精子の成熟 たんぱく質特定 阪大など 不妊治療へ応用期待, 読売新聞 朝刊 2020年7月3日, 2020/07
  • 精子成熟 カギはたんぱく質 阪大など発見 男性不妊治療に応用も, 朝日新聞, 2020/06/10
  • 精子の成熟の調節スイッチをオンにする分子「NELL2」を発見-阪大ほか, QLifePro, 2020/06/08
  • 精子成熟 仕組み解明 大阪大 男性不妊症究明に光, 毎日新聞, 2020/06/05
  • 精子に受精能力を獲得させるスイッチ役たんぱく質を発見 阪大研究グループ発表, 毎日新聞, 2020/06/05
  • 精子成熟の鍵を発見 男性不妊の解明に一歩 大阪大など, 朝日新聞DIGITAL, 2020/06
  • 精子成熟 仕組み解明, 毎日新聞 夕刊 (2020.06.05), 2020/06
  • 精子受精能力、獲得促すたんぱく質 阪大グループ発見 男性不妊症の究明糸口に, 毎日新聞 大阪夕刊 2020年6月8日, 2020/06
  • 大阪大とBaylor医科大、精子の成熟を制御する仕組みをScience誌にて発表, 日経バイオテク 2020年6月10日, 2020/06
  • 精巣で多く発現する遺伝子なのに約7割が生殖能力に関係なし, 科学新聞, 2016/07